CN109168421B - Application of Arabidopsis thaliana in breeding of brassica napus limited inflorescence strain - Google Patents
Application of Arabidopsis thaliana in breeding of brassica napus limited inflorescence strain Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The invention belongs to the technical field of plant breeding methods, and particularly relates to application of brassica carinata in breeding of a brassica napus limited inflorescence maintainer line, a sterile line, a restorer line and a hybrid. The cabbage type rape limited inflorescence maintainer line is obtained by hybridizing the brassica napus serving as a female parent and the brassica napus unlimited inflorescence serving as a male parent to obtain hybrid filial generation, using the hybrid filial generation as a non-recurrent female parent and the brassica napus unlimited inflorescence serving as a recurrent male parent, continuously backcrossing and selfing, and is applied to breeding of a cabbage type rape limited inflorescence sterile line, a cabbage type rape limited inflorescence restorer line and a limited inflorescence hybrid. The limited inflorescence strain of the brassica napus provided by the invention solves the problem of ineffective consumption of increasing the biomass of the brassica napus by using an unlimited inflorescence, reduces the mechanical loss rate, improves the thousand kernel weight, the single plant yield and the yield of the main shaft and the branched silique grains, obviously reduces the lodging rate and provides a new variety for the whole-process mechanized operation of the brassica napus.
Description
Technical Field
The invention belongs to the technical field of plant breeding methods, and particularly relates to application of brassica carinata in breeding of a brassica napus limited inflorescence maintainer line, a sterile line, a restorer line and a hybrid.
Background
Rape is an important oil crop in China, at present, the conventional rape variety is unlimited inflorescence and has a long flowering phase, so that the ripening time of the pod at different levels of the rape plant is inconsistent, and the phenomena that the lower pod is ripe and turns yellow and the upper pod is still immature often occur; early harvest, immature upper siliques, affecting yield formation; the harvest is too late, the lower siliques are easy to crack, the mechanical harvest loss is large, and the mechanical harvest of the rapes is seriously influenced; on the other hand, in the later growth stage of rape, the unlimited inflorescence often causes the pod at the top of the inflorescence to be incapable of normally developing to form an invalid inflorescence, and the phenomenon of abortive inflorescence or no effect axis occurs, so that the invalid consumption of rape biomass is increased, and the yield potential of single-plant seeds is reduced. Therefore, the lack of varieties suitable for mechanized operation is a bottleneck for restricting mechanized production of rape, and the selection of new varieties of rape suitable for mechanized operation has attracted great attention of breeding workers and will gradually become one of the important directions of rape breeding.
In the production of seeds of rape, the sterile line is often used for preparing the rape hybrid, so that the seed production process can be simplified, the production cost of the hybrid seeds is reduced, the quality and the purity of the hybrid are improved, the hybrid has strong and better adaptability, and the utilization range of the hybrid vigor is expanded; the restoring line has normal male and female pistils, can be self-bred to fruit, can pollinate pollen to plants grown from seeds of the sterile line, can restore the fertility of the plants to normal, and has obvious heterosis on characters such as yield and the like; the maintainer line is an indispensable male parent line for which the sterile line depends on passage. The rape maintainer line, sterile line and restorer line three-line hybridization or sterile line and restorer line hybridization can realize advantage complementation, has the advantages of strong excellent combination, strong heterosis, high yield, strong stress resistance, easy seed production and the like, and is widely applied to large-scale production.
The limited inflorescence is a phenomenon that when the plant grows and the inflorescence is generated to a certain degree, the inflorescence meristem is transformed into a flower meristem plant and the top of the inflorescence forms a cluster or a top flower, thereby automatically stopping the growth. The limited inflorescence of the rape can be transferred into rape varieties as a very prominent and stable phenotypic marker character, and the creation of limited inflorescence materials becomes one of effective ways for overcoming the problems brought by the limited inflorescence, but few researches for transferring the limited inflorescence characters into rape three-line hybrids are available.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the problems that the existing rape varieties are not suitable for mechanized operation, the unlimited inflorescence is formed in the later growth stage of the rape to increase the ineffective consumption of the biomass of the rape and the like, so that the application of the brassica carinata in the breeding of the limited inflorescence maintainer line, the sterile line, the restorer line and the hybrid of the brassica napus is provided.
In order to solve the technical problems, the invention adopts the following technical scheme:
the invention provides application of eruca carinata (eruca sativa) in breeding of a brassica napus limited inflorescence maintainer line.
Carrying out hybridization by taking the cabbage type rape unlimited inflorescence as a male parent and the eruca sativa as a female parent to obtain a hybrid progeny F1;
continuously backcrossing and selfing the filial generation F1 serving as a non-recurrent female parent and the cabbage type rape infinite inflorescence strain serving as a male parent to obtain a cabbage type rape finite inflorescence maintainer line;
wherein the non-recurrent female parent is a plant exhibiting the trait of limited inflorescence;
the backcross times are 4-5;
the selfing frequency is 1.
In the breeding process, the illumination time of each day is controlled to be 14-16 h.
In the breeding process, using a potassium permanganate aqueous solution to mix seeds of the brassica carinata; the mass fraction of the potassium permanganate is 0.1-0.5%.
The invention provides application of brassica carinata in breeding of a brassica napus limited inflorescence sterile line.
Hybridizing the cabbage type rape limited inflorescence maintainer line obtained by breeding in the application with a male parent and the cabbage type rape backbone sterile line with a female parent to obtain F2;
carrying out first backcross by taking F2 as a female parent and the cabbage type rape finite inflorescence maintainer line as a male parent to obtain a backcross progeny BC 1;
continuously backcrossing by taking BC1 as a non-recurrent female parent and the cabbage type rape limited inflorescence maintainer line as a recurrent male parent to obtain a cabbage type rape limited inflorescence sterile line;
wherein, the F2 is a plant showing the characters of limited inflorescence and sterility;
the BC1 is a plant showing the characters of limited inflorescence and sterility;
the number of the continuous backcrossing is 4-5.
The invention provides application of brassica carinata in breeding of a cabbage type rape limited inflorescence restorer line.
Hybridizing by using the cabbage type rape limited inflorescence maintainer line obtained by breeding in the application as a female parent and using the cabbage type rape unlimited inflorescence backbone restoring line as a male parent to obtain a hybrid progeny F3; f4 is obtained after F3 is selfed for 1 time;
taking the F4 as a non-recurrent female parent; the cabbage type rape unlimited inflorescence backbone recovery line is a recurrent male parent; carrying out 1 st backcross to obtain backcross offspring;
taking the backcrossed generation as a non-recurrent female parent; recovering the cabbage type rape infinite inflorescence backbone as a recurrent male parent, and carrying out continuous backcross and selfing to obtain a cabbage type rape finite inflorescence recovery line;
wherein, F3 is a plant with a yellow-soil flower color;
the non-recurrent parent is a plant with limited inflorescence character and yellow flower color;
the backcross times are 4 or 5 times, and the selfing times are 2 times.
The invention provides application of brassica carinata in breeding of a brassica napus limited inflorescence hybrid.
Hybridizing the limited inflorescence maintainer line of the cabbage type rape bred in the application with a male parent and the limited inflorescence sterile line of the cabbage type rape bred in the application with a female parent to obtain a limited inflorescence sterile line parent of the cabbage type rape;
and (3) hybridizing by taking the cabbage type rape limited inflorescence sterile line parent as a female parent and taking the cabbage type rape limited inflorescence recovery line obtained by breeding in the application as a male parent to obtain a limited inflorescence hybrid.
The technical scheme of the invention has the following advantages:
1. the invention provides an application of Brassica carica Linn in breeding of a cabbage type rape finite inflorescence maintainer line, which is characterized in that firstly, Brassica carica Linn is used in the breeding process of the cabbage type rape finite inflorescence maintainer line, and the breeding method comprises the steps of taking Brassica carica Linn as a female parent and taking a cabbage type rape infinite inflorescence line as a male parent, and hybridizing to obtain hybrid filial generation; and (3) continuously backcrossing and selfing the hybrid progeny serving as a non-recurrent female parent and the cabbage type rape infinite inflorescence strain serving as a recurrent male parent to obtain the cabbage type rape finite inflorescence maintainer line. The application transfers the limited inflorescence character in the eruca sativa to a cabbage type rape unlimited inflorescence strain, successfully breeds a cabbage type rape limited inflorescence maintainer line, and leads the top ends of the main stem and the branched inflorescence to develop into a flower to terminate the unlimited inflorescence, so that the limited inflorescence is converted into the limited inflorescence, the growth is automatically stopped, and the problem of ineffective consumption of increasing the biomass of the rape by the unlimited inflorescence is solved; compared with the infinite inflorescence strain, the flowering phase of the cabbage type rape finite inflorescence maintainer line can be shortened by 14 days at most, the pod maturity is more consistent, the mechanical loss rate can be reduced by 3.9% at most, the thousand kernel weight of the main shaft and the branch pod grains and the single plant yield are obviously improved, and a new variety is provided for the whole-process mechanized operation of rape.
2. The invention provides an application of Brassica napus in breeding of a cabbage type rape limited inflorescence sterile line, which is characterized in that the Brassica napus is used for the breeding process of the cabbage type rape limited inflorescence sterile line for the first time, the breeding method comprises the steps of hybridizing a cabbage type rape limited inflorescence maintainer line serving as a male parent and a cabbage type backbone sterile line serving as a female parent to obtain a hybrid progeny F2, hybridizing the hybrid progeny F2 serving as a non-recurrent female parent and the cabbage type rape limited inflorescence maintainer line serving as a non-recurrent male parent continuously, and backcrossing to obtain the cabbage type rape limited inflorescence sterile line. The sterile rate of the cabbage type rape limited inflorescence sterile line is 100%, compared with an unlimited inflorescence line, the flowering phase of the cabbage type rape limited inflorescence sterile line can be shortened by 14 days at most, the pod maturity is more consistent, the mechanical loss rate can be reduced by 4.4% at most, the thousand kernel weight of main shaft and branch pod seeds and the yield of a single plant are improved.
3. The invention provides an application of Brassica carica Linn in the breeding of a cabbage type rape limited inflorescence restorer line, the breeding method is to use the cabbage type rape limited inflorescence maintainer line as a female parent and the cabbage type rape unlimited inflorescence backbone restorer line as a male parent to perform hybridization to obtain a hybrid progeny F3; f4 is obtained after F3 is selfed for 1 time; continuously backcrossing and selfing to obtain the restoring line of finite inflorescence of cabbage type rape by using F4 as non-recurrent female parent and cabbage type rape infinite inflorescence backbone restoring line as recurrent male parent. The recovery rate of the cabbage type rape limited inflorescence recovery line is 100 percent; compared with the line of unlimited inflorescence, the flowering phase of the cabbage type rape limited inflorescence restoring line can be shortened by 15 days at most, the maturity of the siliques is more consistent, the mechanical loss rate can be reduced by 3.3 percent at most, and the thousand kernel weight and the single plant yield of the main shaft and the branched siliques are improved.
4. The invention provides an application of Brassica napus in breeding of a cabbage type rape limited inflorescence hybrid.A breeding method is to cross by using a cabbage type rape limited inflorescence maintainer line as a male parent and using a cabbage type rape limited inflorescence sterile line as a female parent to obtain a cabbage type rape limited inflorescence sterile line parent; and hybridizing with the cabbage type rape limited inflorescence sterile line parent as a female parent and the cabbage type rape limited inflorescence restoring line as a male parent to obtain a limited inflorescence hybrid. The purity of the limited inflorescence hybrid is more than 95 percent; compared with the unlimited inflorescence strain, the limited inflorescence hybrid has the advantages that the flowering phase can be shortened by 14 days at most, the silique maturity is more consistent, the mechanical loss rate can be reduced by 5.9 percent at most, the thousand kernel weight of the main shaft and the branched silique grains and the yield of a single plant are improved, the lodging rate is obviously reduced, and the novel cabbage type rape limited inflorescence seed is provided for the production, popularization and application of rape.
5. The application of the brassica carinata in the breeding of the limited inflorescence maintainer line, the sterile line, the restorer line and the hybrid of the brassica napus can adjust the flowering phase of the brassica napus by controlling the illumination time in the breeding process; the germination rate of the brassica napus limited inflorescence strain can be improved by dressing the brassica carinata with a potassium permanganate solution before planting.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is the chromosomal behavior of meiosis of cells of progeny F1 of the cross of the Brassica napus finite inflorescence maintainer line of example 1, wherein A is the number of F1 chromosomes; b is the chromosomal behavior at the final phase of meiosis in the cell; c is the stage I chromosomal behavior in meiosis of cells; d is the stage I chromosomal behavior after meiosis of the cell; e is the stage I chromosomal behavior after meiosis of the cell; f is stage II chromosome behavior in meiosis of the cell; g is the stage II chromosomal behavior after meiosis of the cell;
FIG. 2 is the chromosome behavior of cell meiosis of the backcross progeny BC1 of the brassica napus limited inflorescence sterile line in example 1, wherein A is the number of BC1 chromosomes; b is the chromosomal behavior at the final phase of meiosis in the cell; c is the stage I chromosomal behavior in meiosis of cells; d is the stage I chromosomal behavior after meiosis of the cell; e is the stage I chromosomal behavior after meiosis of the cell; f is the stage II chromosomal behavior after meiosis of the cell.
Detailed Description
The following examples are provided to further understand the present invention, not to limit the scope of the present invention, but to provide the best mode, not to limit the content and the protection scope of the present invention, and any product similar or similar to the present invention, which is obtained by combining the present invention with other prior art features, falls within the protection scope of the present invention.
The examples do not show the specific experimental steps or conditions, and can be performed according to the conventional experimental steps described in the literature in the field. The reagents or instruments used are not indicated by manufacturers, and are all conventional reagent products which can be obtained commercially.
Example 1
All breeding processes of the embodiment are carried out under natural conditions;
the embodiment provides an application of brassica carinata in breeding of a brassica napus limited inflorescence maintainer line, and the breeding method specifically comprises the following steps:
in 3 months of 2010, 1 part of eruca sativa is taken as a female parent and 1 part of cabbage type rape unlimited inflorescence strain (Zhongshuang 11) is taken as a male parent in Nanchang in the west of the Jiangxi, hybridization is carried out, plants with limited inflorescence characters are screened from the female parent, and 1 part of hybrid progeny F1 is obtained;
in 7 months in 2010, carrying out first backcross on Qinghai Xining by taking 1 part of filial generation F1 as a female parent and 1 part of Zhongshuang 11 as a male parent to obtain 5 parts of backcross progeny;
in 3 months of 2011, in Jiangxi Nanchang, 5 parts of backcross progeny are used as female parents, 1 part of Zhongshuang 11 is used as male parents, and the second backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2011, 10 backcross progeny are used as a female parent and 1 medium double 11 is used as a male parent in Qinghai Xining to carry out third backcross to obtain 30 backcross progeny;
in 3 months of 2012, 30 parts of backcross progeny are taken as female parents in southern Chang in Jiangxi, and 1 part of Zhongshuang 11 is taken as male parents to carry out fourth backcross to obtain 50 parts of backcross progeny;
and 7, in 2012, in Qinghai Xining, 50 parts of backcross progeny are used as female parents, 1 part of Zhongshuang 11 is used as male parents, the fifth backcross is carried out, and then the 1 generation is selfed to obtain 80 parts of cabbage type rape limited inflorescence maintainer lines.
The embodiment also provides an application of the brassica carinata in the breeding of the brassica napus limited inflorescence sterile line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape backbone sterile line (RF02-004) is taken as a female parent, and hybridization is carried out to obtain 1 part of hybrid progeny F2;
in 7 months in 2013, Qinghai Xining, 1 part of F2 is taken as a non-recurrent female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a recurrent male parent, and first backcross is carried out to obtain 20 parts of backcross progeny BC 1;
in 3 months in 2014, Jiangxi Nanchang, 20 parts of BC1 are used as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is used as a male parent, and second backcross is carried out to obtain 50 parts of backcross progeny BC 2;
in 7 months in 2014, Qinghai Xining, 50 parts of BC2 are taken as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, and the third backcross is carried out to obtain 100 parts of backcross progeny BC 3;
performing backcross for the fourth time by taking 100 parts of BC3 as a female parent and 1 part of cabbage type rape finite inflorescence maintainer line as a male parent in Nanchang Jiangxi in 3 months of 2015 to obtain 100 parts of backcross progeny BC 4;
and in 2015, in 7 months, Qinghai Xining, 100 parts of BC4 are used as a female parent, 1 part of cabbage type rape limited inflorescence maintainer line is used as a male parent, and the fifth backcross is carried out to obtain 100 parts of cabbage type rape limited inflorescence sterile line.
The embodiment also provides an application of the brassica carinata in breeding of a brassica napus limited inflorescence restorer line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is used as a female parent, 1 part of cabbage type rape infinite inflorescence backbone restorer line (C01) is used as a male parent, and hybridization is carried out to obtain 1 part of hybrid progeny F3;
selfing 1 part of F3 to obtain 1 part of F4 in 7 months in 2013 and Nanchang Jiangxi;
in 3 months of 2014, Qinghai Xining, 1 part of F4 is taken as a non-recurrent female parent, 1 part of C01 is taken as a recurrent male parent, and the 1 st backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2014, Jiangxi Nanchang, 10 parts of backcross progeny are used as female parents, 1 part of C01 is used as male parents, and second backcross is carried out to obtain 20 parts of backcross progeny;
in 2015, in 3 months, Qinghai Xining, 20 parts of backcross progeny are used as a female parent, 1 part of C01 is used as a male parent, and third backcross is carried out to obtain 50 parts of backcross progeny;
carrying out fourth backcross on the filial generation of 50 parts as a female parent and 1 part of C01 as a male parent in Nanchang Jiangxi in 7 months in 2015 to obtain 100 parts of backcross filial generation;
and (3) in 2016, carrying out continuous selfing on backcross progeny for 2 generations to obtain 100 parts of the cabbage type rape finite inflorescence restoring line.
The embodiment also provides application of the brassica carica linn in breeding of the brassica napus limited inflorescence hybrid, and the breeding method specifically comprises the following steps:
in 7 months in 2016, in Xining of Qinghai, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape finite inflorescence sterile line is taken as a female parent, and 10 parts of cabbage type rape finite inflorescence sterile line parents are obtained by hybridization; in 3 months in 2017, in southern Chang, Jiangxi, 10 parts of cabbage type rape limited inflorescence sterile line parent are used as a female parent, 1 part of cabbage type rape limited inflorescence restoration line is used as a male parent, and hybridization is carried out to obtain 50 parts of limited inflorescence hybrid.
Fig. 1 shows the chromosome behavior of meiosis of cells of progeny F1 of the brassica napus limited inflorescence maintainer line hybrid, which can be obtained from a graph, wherein the number of chromosomes, 2n, is 36; in panel B, the occurrence of monovalent, divalent, trivalent, or tetravalent species in the terminal phase pairing is observed; chromosome lagging behind behavior at stage I is observed in panel C; later stage I separation, 17:19 separation, is observed in panel D; chromosome lagging behind behavior at later stage I is observed in panel E; chromosome lagging behind behavior at the middle stage II is observed in the F picture; the G plot shows that chromosome lagging behind behavior occurs in later stage II.
FIG. 2 shows the chromosome behavior of the cell meiosis of the backcross progeny BC1 of the Brassica napus limited inflorescence sterile line, which can be obtained from A picture, wherein the number of chromosomes is 2 n-38; in panel B, 19 pairs of bivalent bodies are observed in terminal transition; c, observed in the picture that the chromosome of the middle stage I does not fall behind; in panel D, 19:19 was observed in later stage I, and no chromosome lag was observed; in panel E, 18:20 segregation is observed, and chromosome lag is not observed; in the F plot, normal terminal phase II segregation was observed, with no chromosome lagging behind behavior.
Example 2
In the breeding process of the embodiment, the illumination time of the plant is 15h every day, when the illumination is insufficient, the plant is illuminated by lamplight, and the intensity of the lamplight is controlled to be the same as that of natural light;
the embodiment provides an application of brassica carinata in breeding of a brassica napus limited inflorescence maintainer line, and the breeding method specifically comprises the following steps:
in 3 months of 2010, 1 part of eruca sativa is taken as a female parent and 1 part of cabbage type rape unlimited inflorescence strain (Zhongshuang 11) is taken as a male parent in Nanchang in the west of the Jiangxi, hybridization is carried out, plants with limited inflorescence characters are screened from the female parent, and 1 part of hybrid progeny F1 is obtained;
in 7 months in 2010, carrying out first backcross on Qinghai Xining by taking 1 part of filial generation F1 as a female parent and 1 part of Zhongshuang 11 as a male parent to obtain 5 parts of backcross progeny;
in 3 months of 2011, in Jiangxi Nanchang, 5 parts of backcross progeny are used as female parents, 1 part of Zhongshuang 11 is used as male parents, and the second backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2011, 10 backcross progeny are used as a female parent and 1 medium double 11 is used as a male parent in Qinghai Xining to carry out third backcross to obtain 30 backcross progeny;
in 3 months of 2012, 30 parts of backcross progeny are taken as female parents in southern Chang in Jiangxi, and 1 part of Zhongshuang 11 is taken as male parents to carry out fourth backcross to obtain 50 parts of backcross progeny;
and 7, in 2012, in Qinghai Xining, 50 parts of backcross progeny are taken as female parents, 1 part of Zhongshuang 11 is taken as male parents, the fifth backcross is carried out, and then the 1 generation is selfed to obtain 100 parts of cabbage type rape limited inflorescence maintainer lines.
The embodiment also provides an application of the brassica carinata in the breeding of the brassica napus limited inflorescence sterile line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape backbone sterile line (RF02-004) is taken as a female parent, and hybridization is carried out to obtain 1 part of hybrid progeny F2;
in 7 months in 2013, Qinghai Xining, 1 part of F2 is taken as a non-recurrent female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a recurrent male parent, and first backcross is carried out to obtain 20 parts of backcross progeny BC 1;
in 3 months in 2014, Jiangxi Nanchang, 20 parts of BC1 are used as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is used as a male parent, and second backcross is carried out to obtain 50 parts of backcross progeny BC 2;
in 7 months in 2014, Qinghai Xining, 50 parts of BC2 are taken as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, and the third backcross is carried out to obtain 100 parts of backcross progeny BC 3;
performing backcross for the fourth time by taking 100 parts of BC3 as a female parent and 1 part of cabbage type rape finite inflorescence maintainer line as a male parent in Nanchang Jiangxi in 3 months of 2015 to obtain 100 parts of backcross progeny BC 4;
and in 2015, in 7 months, Qinghai Xining, 100 parts of BC4 are used as a female parent, 1 part of cabbage type rape limited inflorescence maintainer line is used as a male parent, and the fifth backcross is carried out to obtain 100 parts of cabbage type rape limited inflorescence sterile line.
The embodiment also provides an application of the brassica carinata in breeding of a brassica napus limited inflorescence restorer line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is used as a female parent, 1 part of cabbage type rape infinite inflorescence backbone restorer line (C01) is used as a male parent, and hybridization is carried out to obtain 1 part of hybrid progeny F3;
selfing 1 part of F3 to obtain 1 part of F4 in 7 months in 2013 and Nanchang Jiangxi;
in 3 months of 2014, Qinghai Xining, 1 part of F4 is taken as a non-recurrent female parent, 1 part of C01 is taken as a recurrent male parent, and the 1 st backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2014, Jiangxi Nanchang, 10 parts of backcross progeny are used as female parents, 1 part of C01 is used as male parents, and second backcross is carried out to obtain 20 parts of backcross progeny;
in 2015, in 3 months, Qinghai Xining, 20 parts of backcross progeny are used as a female parent, 1 part of C01 is used as a male parent, and third backcross is carried out to obtain 50 parts of backcross progeny;
carrying out fourth backcross on the filial generation of 50 parts as a female parent and 1 part of C01 as a male parent in Nanchang Jiangxi in 7 months in 2015 to obtain 100 parts of backcross filial generation;
and (3) in 2016, carrying out continuous selfing on backcross progeny for 2 generations to obtain 100 parts of the cabbage type rape finite inflorescence restoring line.
The embodiment also provides application of the brassica carica linn in breeding of the brassica napus limited inflorescence hybrid, and the breeding method specifically comprises the following steps:
in 7 months in 2016, in Xining of Qinghai, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape finite inflorescence sterile line is taken as a female parent, and 10 parts of cabbage type rape finite inflorescence sterile line parents are obtained by hybridization; in 3 months in 2017, in southern Chang, Jiangxi, 10 parts of cabbage type rape limited inflorescence sterile line parent are used as a female parent, 1 part of cabbage type rape limited inflorescence restoration line is used as a male parent, and hybridization is carried out to obtain 50 parts of limited inflorescence hybrid.
Example 3
All breeding processes of the embodiment are carried out under natural conditions;
the embodiment provides an application of brassica carinata in breeding of a cabbage type rape finite inflorescence maintainer line, before planting, potassium permanganate aqueous solution with the mass fraction of 0.5% is used for soaking brassica carinata seeds, and the seeds are dried after 6 hours, and the breeding method specifically comprises the following steps:
in 3 months of 2010, 1 part of eruca sativa is taken as a female parent and 1 part of cabbage type rape unlimited inflorescence strain (Zhongshuang 11) is taken as a male parent in Nanchang in the west of the Jiangxi, hybridization is carried out, plants with limited inflorescence characters are screened from the female parent, and 1 part of hybrid progeny F1 is obtained;
in 7 months in 2010, carrying out first backcross on Qinghai Xining by taking 1 part of filial generation F1 as a female parent and 1 part of Zhongshuang 11 as a male parent to obtain 5 parts of backcross progeny;
in 3 months of 2011, in Jiangxi Nanchang, 5 parts of backcross progeny are used as female parents, 1 part of Zhongshuang 11 is used as male parents, and the second backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2011, 10 backcross progeny are used as a female parent and 1 medium double 11 is used as a male parent in Qinghai Xining to carry out third backcross to obtain 30 backcross progeny;
in 3 months of 2012, 30 parts of backcross progeny are taken as female parents in southern Chang in Jiangxi, and 1 part of Zhongshuang 11 is taken as male parents to carry out fourth backcross to obtain 50 parts of backcross progeny;
and 7, in 2012, in Qinghai Xining, 50 parts of backcross progeny are taken as female parents, 1 part of Zhongshuang 11 is taken as male parents, the fifth backcross is carried out, and then the 1 generation is selfed to obtain 100 parts of cabbage type rape limited inflorescence maintainer lines.
The embodiment also provides an application of the brassica carinata in the breeding of the brassica napus limited inflorescence sterile line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape backbone sterile line (RF02-004) is taken as a female parent, and hybridization is carried out to obtain 1 part of hybrid progeny F2;
in 7 months in 2013, Qinghai Xining, 1 part of F2 is taken as a non-recurrent female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a recurrent male parent, and first backcross is carried out to obtain 20 parts of backcross progeny BC 1;
in 3 months in 2014, Jiangxi Nanchang, 20 parts of BC1 are used as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is used as a male parent, and second backcross is carried out to obtain 50 parts of backcross progeny BC 2;
in 7 months in 2014, Qinghai Xining, 50 parts of BC2 are taken as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, and the third backcross is carried out to obtain 100 parts of backcross progeny BC 3;
performing backcross for the fourth time by taking 100 parts of BC3 as a female parent and 1 part of cabbage type rape finite inflorescence maintainer line as a male parent in Nanchang Jiangxi in 3 months of 2015 to obtain 100 parts of backcross progeny BC 4;
and in 2015, in 7 months, Qinghai Xining, 100 parts of BC4 are used as a female parent, 1 part of cabbage type rape limited inflorescence maintainer line is used as a male parent, and the fifth backcross is carried out to obtain 100 parts of cabbage type rape limited inflorescence sterile line.
The embodiment also provides an application of the brassica carinata in breeding of a brassica napus limited inflorescence restorer line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is used as a female parent, 1 part of cabbage type rape infinite inflorescence backbone restorer line (C01) is used as a male parent, and hybridization is carried out to obtain 1 part of hybrid progeny F3;
selfing 1 part of F3 to obtain 1 part of F4 in 7 months in 2013 and Nanchang Jiangxi;
in 3 months of 2014, Qinghai Xining, 1 part of F4 is taken as a non-recurrent female parent, 1 part of C01 is taken as a recurrent male parent, and the 1 st backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2014, Jiangxi Nanchang, 10 parts of backcross progeny are used as female parents, 1 part of C01 is used as male parents, and second backcross is carried out to obtain 20 parts of backcross progeny;
in 2015, in 3 months, Qinghai Xining, 20 parts of backcross progeny are used as a female parent, 1 part of C01 is used as a male parent, and third backcross is carried out to obtain 50 parts of backcross progeny;
carrying out fourth backcross on the filial generation of 50 parts as a female parent and 1 part of C01 as a male parent in Nanchang Jiangxi in 7 months in 2015 to obtain 100 parts of backcross filial generation;
and (3) in 2016, carrying out continuous selfing on backcross progeny for 2 generations to obtain 100 parts of the cabbage type rape finite inflorescence restoring line.
The embodiment also provides application of the brassica carica linn in breeding of the brassica napus limited inflorescence hybrid, and the breeding method specifically comprises the following steps:
in 7 months in 2016, in Xining of Qinghai, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape finite inflorescence sterile line is taken as a female parent, and 10 parts of cabbage type rape finite inflorescence sterile line parents are obtained by hybridization; in 3 months in 2017, in southern Chang, Jiangxi, 10 parts of cabbage type rape limited inflorescence sterile line parent are used as a female parent, 1 part of cabbage type rape limited inflorescence restoration line is used as a male parent, and hybridization is carried out to obtain 50 parts of limited inflorescence hybrid.
Example 4
In the breeding process of the embodiment, the illumination time is 16h every day, when the illumination is insufficient, the illumination is carried out by using lamplight, and the intensity of the lamplight is controlled to be the same as that of natural light;
the embodiment provides an application of brassica carinata in breeding of a cabbage type rape finite inflorescence maintainer line, before planting, potassium permanganate aqueous solution with the mass fraction of 0.1% is used for soaking brassica carinata seeds, and the seeds are dried after 4 hours, and the method specifically comprises the following steps:
in 3 months of 2010, 1 part of eruca sativa is taken as a female parent and 1 part of cabbage type rape unlimited inflorescence strain (Zhongshuang 11) is taken as a male parent in Nanchang in the west of the Jiangxi, hybridization is carried out, plants with limited inflorescence characters are screened from the female parent, and 1 part of hybrid progeny F1 is obtained;
in 7 months in 2010, carrying out first backcross on Qinghai Xining by taking 1 part of filial generation F1 as a female parent and 1 part of Zhongshuang 11 as a male parent to obtain 5 parts of backcross progeny;
in 3 months of 2011, in Jiangxi Nanchang, 5 parts of backcross progeny are used as female parents, 1 part of Zhongshuang 11 is used as male parents, and the second backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2011, 10 backcross progeny are used as a female parent and 1 medium double 11 is used as a male parent in Qinghai Xining to carry out third backcross to obtain 30 backcross progeny;
in 3 months of 2012, 30 parts of backcross progeny are taken as female parents in southern Chang in Jiangxi, and 1 part of Zhongshuang 11 is taken as male parents to carry out fourth backcross to obtain 50 parts of backcross progeny;
and 7, in 2012, in Qinghai Xining, 50 parts of backcross progeny are taken as female parents, 1 part of Zhongshuang 11 is taken as male parents, the fifth backcross is carried out, and then the 1 generation is selfed to obtain 100 parts of cabbage type rape limited inflorescence maintainer lines.
The embodiment also provides an application of the brassica carinata in the breeding of the brassica napus limited inflorescence sterile line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape backbone sterile line (RF02-004) is taken as a female parent, and hybridization is carried out to obtain 1 part of hybrid progeny F2;
in 7 months in 2013, Qinghai Xining, 1 part of F2 is taken as a non-recurrent female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a recurrent male parent, and first backcross is carried out to obtain 20 parts of backcross progeny BC 1;
in 3 months in 2014, Jiangxi Nanchang, 20 parts of BC1 are used as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is used as a male parent, and second backcross is carried out to obtain 50 parts of backcross progeny BC 2;
in 7 months in 2014, Qinghai Xining, 50 parts of BC2 are taken as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, and the third backcross is carried out to obtain 100 parts of backcross progeny BC 3;
performing backcross for the fourth time by taking 100 parts of BC3 as a female parent and 1 part of cabbage type rape finite inflorescence maintainer line as a male parent in Nanchang Jiangxi in 3 months of 2015 to obtain 100 parts of backcross progeny BC 4;
and in 2015, in 7 months, Qinghai Xining, 100 parts of BC4 are used as a female parent, 1 part of cabbage type rape limited inflorescence maintainer line is used as a male parent, and the fifth backcross is carried out to obtain 100 parts of cabbage type rape limited inflorescence sterile line.
The embodiment also provides an application of the brassica carinata in breeding of a brassica napus limited inflorescence restorer line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is used as a female parent, 1 part of cabbage type rape infinite inflorescence backbone restorer line (C01) is used as a male parent, and hybridization is carried out to obtain 1 part of hybrid progeny F3;
selfing 1 part of F3 to obtain 1 part of F4 in 7 months in 2013 and Nanchang Jiangxi;
in 3 months of 2014, Qinghai Xining, 1 part of F4 is taken as a non-recurrent female parent, 1 part of C01 is taken as a recurrent male parent, and the 1 st backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2014, Jiangxi Nanchang, 10 parts of backcross progeny are used as female parents, 1 part of C01 is used as male parents, and second backcross is carried out to obtain 20 parts of backcross progeny;
in 2015, in 3 months, Qinghai Xining, 20 parts of backcross progeny are used as a female parent, 1 part of C01 is used as a male parent, and third backcross is carried out to obtain 50 parts of backcross progeny;
carrying out fourth backcross on the filial generation of 50 parts as a female parent and 1 part of C01 as a male parent in Nanchang Jiangxi in 7 months in 2015 to obtain 100 parts of backcross filial generation;
and (3) in 2016, carrying out continuous selfing on backcross progeny for 2 generations to obtain 100 parts of the cabbage type rape finite inflorescence restoring line.
The embodiment also provides application of the brassica carica linn in breeding of the brassica napus limited inflorescence hybrid, and the breeding method specifically comprises the following steps:
in 7 months in 2016, in Xining of Qinghai, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape finite inflorescence sterile line is taken as a female parent, and 10 parts of cabbage type rape finite inflorescence sterile line parents are obtained by hybridization; in 3 months in 2017, in southern Chang, Jiangxi, 10 parts of cabbage type rape limited inflorescence sterile line parent are used as a female parent, 1 part of cabbage type rape limited inflorescence restoration line is used as a male parent, and hybridization is carried out to obtain 50 parts of limited inflorescence hybrid.
Example 5
All breeding processes of the embodiment are carried out under natural conditions;
the embodiment provides an application of brassica carinata in breeding of a brassica napus limited inflorescence maintainer line, and the breeding method specifically comprises the following steps:
in 3 months of 2010, 1 part of eruca sativa is taken as a female parent and 1 part of cabbage type rape (Deyou 5) is taken as a male parent in Nanchang in the west of the Jiangxi, hybridization is carried out, plants with limited inflorescence characters are screened from the female parent, and 1 part of hybrid progeny F1 is obtained;
in 7 months in 2010, carrying out first backcross on Qinghai Xining by taking 1 part of hybrid progeny F1 as a female parent and 1 part of De oil No. 5 as a male parent to obtain 5 parts of backcross progeny;
in 3 months of 2011, in Jiangxi Nanchang, 5 parts of backcross progeny are used as female parents, 1 part of De-YOU No. 5 is used as male parents, and second backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months of 2011, in Qinghai Xining, 10 parts of backcross progeny are used as a female parent, 1 part of De-YOU No. 5 is used as a male parent, and the third backcross is carried out to obtain 30 parts of backcross progeny;
in 3 months of 2012, 30 parts of backcross progeny are taken as female parents in southern Chang of Jiangxi and 1 part of De-YOU No. 5 is taken as male parents to carry out the fourth backcross to obtain 50 parts of backcross progeny;
and 7, in 2012, in Qinghai Xining, 50 parts of backcross progeny are taken as a female parent, 1 part of De-YOU No. 5 is taken as a male parent, the fifth backcross is carried out, and then the 1 generation is selfed to obtain 100 parts of cabbage type rape finite inflorescence maintainer line.
The embodiment also provides an application of the brassica carinata in the breeding of the brassica napus limited inflorescence sterile line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape backbone sterile line (17A) is taken as a female parent, and hybridization is carried out to obtain 1 part of hybrid progeny F2;
in 7 months in 2013, Qinghai Xining, 1 part of F2 is taken as a non-recurrent female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a recurrent male parent, and first backcross is carried out to obtain 20 parts of backcross progeny BC 1;
in 3 months in 2014, Jiangxi Nanchang, 20 parts of BC1 are used as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is used as a male parent, and second backcross is carried out to obtain 50 parts of backcross progeny BC 2;
in 7 months in 2014, Qinghai Xining, 50 parts of BC2 are taken as a female parent, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, and the third backcross is carried out to obtain 100 parts of backcross progeny BC 3;
performing backcross for the fourth time by taking 100 parts of BC3 as a female parent and 1 part of cabbage type rape finite inflorescence maintainer line as a male parent in Nanchang Jiangxi in 3 months of 2015 to obtain 100 parts of backcross progeny BC 4;
and in 2015, in 7 months, Qinghai Xining, 100 parts of BC4 are used as a female parent, 1 part of cabbage type rape limited inflorescence maintainer line is used as a male parent, and the fifth backcross is carried out to obtain 100 parts of cabbage type rape limited inflorescence sterile line.
The embodiment also provides an application of the brassica carinata in breeding of a brassica napus limited inflorescence restorer line, and the breeding method specifically comprises the following steps:
in 3 months in 2013, Jiangxi Nanchang, 1 part of cabbage type rape finite inflorescence maintainer line is used as a female parent, 1 part of cabbage type rape infinite inflorescence backbone restorer line (HF16-1400R) is used as a male parent, and hybridization is carried out to obtain 1 part of hybrid progeny F3;
selfing 1 part of F3 to obtain 1 part of F4 in 7 months in 2013 and Nanchang Jiangxi;
in 3 months of 2014, Qinghai Xining, 1 part of F4 is taken as a non-recurrent female parent, 1 part of HF16-1400R is taken as a recurrent male parent, and the 1 st backcross is carried out to obtain 10 parts of backcross progeny;
in 7 months in 2014, Jiangxi Nanchang, 10 parts of backcross progeny are used as female parents, 1 part of HF16-1400R is used as male parents, and second backcross is carried out to obtain 20 parts of backcross progeny;
in 2015, in 3 months, Qinghai Xining, 20 parts of backcross progeny are used as a female parent, 1 part of HF16-1400R is used as a male parent, and third backcross is carried out to obtain 50 parts of backcross progeny;
carrying out backcross for the fourth time by taking 50 parts of backcross progeny as a female parent and 1 part of HF16-1400R as a male parent in Nanchang Jiangxi in 7 months in 2015 to obtain 100 parts of backcross progeny;
and (3) in 2016, carrying out continuous selfing on backcross progeny for 2 generations to obtain 100 parts of the cabbage type rape finite inflorescence restoring line.
The invention also provides an application of the brassica carinata in the breeding of the brassica napus limited inflorescence hybrid, and the breeding method specifically comprises the following steps:
in 7 months in 2016, in Xining of Qinghai, 1 part of cabbage type rape finite inflorescence maintainer line is taken as a male parent, 1 part of cabbage type rape finite inflorescence sterile line is taken as a female parent, and 10 parts of cabbage type rape finite inflorescence sterile line parents are obtained by hybridization; in 3 months in 2017, in southern Chang, Jiangxi, 10 parts of cabbage type rape limited inflorescence sterile line parent are used as a female parent, 1 part of cabbage type rape limited inflorescence restoration line is used as a male parent, and hybridization is carried out to obtain 50 parts of limited inflorescence hybrid.
In 7 months in 2017, in southern chang, 20 lines of the brassica napus limited inflorescence maintainer line, the brassica napus limited inflorescence sterile line, the brassica napus limited inflorescence restorer line and the limited inflorescence hybrid obtained in example 1, example 2, example 3, example 4 and example 5 are respectively planted in a field with the area of 1 mu; meanwhile, a cabbage type unlimited inflorescence line (RS013-008) is selected as a comparative example, and the planting conditions are the same as those of a limited inflorescence line. After harvesting, the results of statistics and measurements are shown in Table 1.
TABLE 1 statistical measurement results of examples 1-5 and comparative examples
The statistical measurement results show that compared with the cabbage unlimited inflorescence strain, the flowering phase of the cabbage limited inflorescence strain can be shortened by 15 days at most, the thousand kernel weight of the main shaft and the branch silique grains, the single plant yield and the yield are obviously improved, the mechanical loss rate can be reduced by 5.9 percent at most, and the mechanized operation is facilitated; the limited inflorescence strain has no invalid inflorescence and invalid silique, and the problem of invalid consumption of increasing the biomass of the rapes by the unlimited inflorescence is better solved.
In the breeding process, the sterile rate of the cabbage type rape limited inflorescence sterile line is 100 percent, the recovery rate of the cabbage type rape limited inflorescence restoring line is 100 percent, and the purity of the limited inflorescence hybrid is more than 95 percent, thereby providing novel cabbage type rape limited inflorescence seeds for the production, popularization and application of rape.
In addition, compared with example 2, example 1 shows that the florescence is shortened by controlling the illumination in the breeding process; compared with the example 3, the example 1 shows that the seed dressing treatment of the seeds by using potassium permanganate before planting in the breeding process is beneficial to improving the germination rate; compared with example 4, the example 1 shows that the flowering period is shortened and the germination rate of the seeds is improved by controlling illumination and carrying out seed dressing treatment in the breeding process.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (6)
1. The application of the Arabidopsis thaliana in the breeding of the limited inflorescence maintainer line of the Brassica napus is characterized by comprising the following steps of,
carrying out hybridization by taking the cabbage type rape unlimited inflorescence as a male parent and taking the Ethiobium aegypti as a female parent to obtain a hybrid progeny F1;
continuously backcrossing and selfing the filial generation F1 serving as a non-recurrent female parent and the cabbage type rape infinite inflorescence strain serving as a male parent to obtain a cabbage type rape finite inflorescence maintainer line;
wherein the non-recurrent female parent is a plant exhibiting the trait of limited inflorescence;
the backcross times are 4-5; the selfing frequency is 1.
2. The use of Arabidopsis thaliana of claim 1 for the selective breeding of Brassica napus limited inflorescence maintainer lines wherein the illumination time per day is controlled to be in the range of 14h to 16 h.
3. Use of the brassica carinata according to claim 1 for the selective breeding of brassica oleracea limited inflorescence maintainer lines, wherein seeds of the brassica carinata are dressed with an aqueous solution of potassium permanganate; the mass fraction of the potassium permanganate is 0.1-0.5%.
4. An application of the Arabidopsis thaliana in the breeding of the limited inflorescence sterile line of the Brassica napus is characterized by comprising the following steps,
hybridizing a cabbage type rape finite inflorescence maintainer line obtained by breeding in the application of any one of claims 1-3 as a male parent and a cabbage type rape backbone sterile line as a female parent to obtain F2;
carrying out first backcross by taking the F2 as a female parent and the cabbage type rape finite inflorescence maintainer line as a male parent to obtain a backcross progeny BC 1;
continuously backcrossing by taking BC1 as a non-recurrent female parent and the cabbage type rape limited inflorescence maintainer line as a recurrent male parent to obtain a cabbage type rape limited inflorescence sterile line;
wherein, the F2 is a plant showing the characters of limited inflorescence and sterility;
the BC1 is a plant showing the characters of limited inflorescence and sterility;
the number of the continuous backcrossing is 4-5.
5. The application of the brassica carinata in the breeding of the brassica napus limited inflorescence restorer line is characterized in that the breeding method specifically comprises the following steps:
crossing with the cabbage type rape finite inflorescence maintainer line obtained by breeding in the application of any one of claims 1-3 as a female parent and the cabbage type rape infinite inflorescence backbone restorer line as a male parent to obtain a hybrid progeny F3; f4 is obtained after F3 is selfed for 1 time;
taking the F4 as a non-recurrent female parent; the cabbage type rape unlimited inflorescence backbone recovery line is a recurrent male parent; carrying out 1 st backcross to obtain backcross offspring;
taking the backcrossed generation as a non-recurrent female parent; recovering the cabbage type rape infinite inflorescence backbone as a recurrent male parent, and carrying out continuous backcross and selfing to obtain a cabbage type rape finite inflorescence recovery line;
wherein, F3 is a plant with a yellow-soil flower color;
the non-recurrent female parent is a plant with limited inflorescence character and yellow flower color;
the backcross times are 4 or 5 times, and the selfing times are 2 times.
6. An application of the Arabidopsis thaliana in the breeding of the limited inflorescence hybrid of the Brassica napus is characterized by comprising the following steps,
hybridizing a cabbage type rape limited inflorescence maintainer line obtained by breeding in any one of the applications of claim 1-3 as a male parent and a cabbage type rape limited inflorescence sterile line obtained by breeding in the application of claim 4 as a female parent to obtain a cabbage type rape limited inflorescence sterile line parent;
crossing with the cabbage type rape limited inflorescence sterile line parent as female parent and the cabbage type rape limited inflorescence restoring line obtained through breeding in the application of claim 5 as male parent to obtain limited inflorescence hybrid.
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