CN101513168A - Method for artificially developing novel brassica napus - Google Patents
Method for artificially developing novel brassica napus Download PDFInfo
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- CN101513168A CN101513168A CNA200910103551XA CN200910103551A CN101513168A CN 101513168 A CN101513168 A CN 101513168A CN A200910103551X A CNA200910103551X A CN A200910103551XA CN 200910103551 A CN200910103551 A CN 200910103551A CN 101513168 A CN101513168 A CN 101513168A
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- cabbage
- type rape
- brassica napus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/13—Abiotic stress
- Y02A40/132—Plants tolerant to drought
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Abstract
The present invention provides a method for artificially developing novel brassica napus. The excellent characters of cabbage and mustard type rape are integrated for obtaining novel brassica napus. The hybridization compatibility when the mustard type rape and cabbage are used for executing interspecific hybridization is higher than the hybridization compatibility when the Chinese cabbage and cabbage are used for executing interspecific hybridization. The interspecific hybrid is easily obtained, and simultaneously, the advantages of drought resistance, cold resistance, excellent strain type, etc. of the mustard type rape can be introduced into the brassica napus. The method of the invention also has the advantages of stable heredity, high speed, large area of character variation and facilitation for selecting the brassica napus with excellent charcter. Furthermore, the chromosomes of AC chromosome set of brassica napus obtained from the method of the invention respectively come from the mustard type rape and cabbage, have larger difference from the prior brassica napus. The use of hybrid vigor of genome of brassica napus is facilitated and the production level of cabbage is increased.
Description
Technical field
The present invention relates to the plant breeding technique field, particularly relate to the synthetic new way of a kind of cabbage type rape (2n=4x=AACC=38), specifically is to utilize wild cabbage (2n=2x=C ° C °=18) and mustard type rape (2n=4x=A
JA
JB
JB
J=34) method of the novel cabbage type rape of interspecific cross synthetic (2n=4x=A ' A ' C ' C '=38).
Background technology
The rape conduct is one of main oil crop in the world, are not only important edible oil sources and protein feeds, and are the important raw material of industry and nectariferous plants.China is one of country that carries out the earliest rape cultivation, but what mainly carry out in the past is the turnip type rape cultivation.In the thirties 20th century of eighties of last century, cabbage type rape begins to introduce and cultivate from Korea, Japan and Britain, now be distributed widely in all parts of the country, it is the most concentrated to distribute with rape main producing region, each province, the Yangtze river basin especially, becomes one of most important oil crop of China.Along with the raising of people's living standard and deepening continuously of rapeseed breeding research work, people have higher requirement to the rapeseed breeding target, and the rape improved seeds that the high yield and high quality of seed selection floorboard with high oil content, low erucic acid, low sulfatide resists more become the main goal of attack of current rapeseed breeding.But the cabbage type rape of China's rape commerial growing at present is at first from external introducing, and the time of introducing is short, and used " two low " (low erucic acid, the low sulfatide) conventional variety and the genetic origin of Hybrid also come from abroad on producing.Therefore, China cabbage type rape inbred genetic basis is narrow, and resource is also along with the large-scale popularization of part kind is exhausted day by day in planting.At this situation, utilize rape to belong to interspecies relation research, import other kind (genus) beneficial gene by distant hybridization, thereby widen cabbage type rape genetic breeding gene pool, it is important to seem all the more.
Rape belongs to by Chinese cabbage (B.campestris L., 2n=AA=20), wild cabbage (B.oleracea L.2n=CC=18), black mustard (B.nigra L, 2n=BB=16) 3 elementary species and cabbage type rape (B.napus L., 2n=AACC=38), brassicacarinata (B.carinata Braun L.2n=BBCC=34), leaf mustard (B.juncea Czern﹠amp; Coss L., 2n=AABB=36) 3 aggregate species are formed.Cabbage type rape mainly is by natural hybrization between wild cabbage and Chinese cabbage kind, forms after the chromosome doubling, and this also becomes present artificially synthesized Brassica type rape, creates the main path of new germ plasm resource.There are defectives such as the affine difficulty of hybridization, hybrid generation's (synthetic cabbage type rape) inheritance stability progress are slow, the genetic variation amplitude is less by this kind approach artificially synthesized Brassica type rape, because its parent consists of wild cabbage and Chinese cabbage, two parents are that dish is used the type crop, therefore are difficult to choose good tree characteristics.In addition, also have two kinds of novel cabbage type rape approach of seed selection at present in breeding, promptly by cabbage type rape and turnip type rape, cabbage type rape and mustard type rape interspecific cross, seed selection obtains cabbage type rape in the offspring respectively; These two kinds of approach cross compatibilities are better, work is simple, but one of parent of these two kinds of approach is itself is exactly natural cabbage type rape, therefore the cabbage type rape hereditary basis that obtains among its a large amount of separation offsprings is narrow relatively, though also obtained new material and the new varieties on producing, used in a large number both at home and abroad in this way, but these materials and natural cabbage type rape are compared, and its genetic distance is nearer relatively, are unfavorable for the utilization of sub-gene group species hybrid advantage.
Up to now, wild cabbage and mustard type rape merit are concentrated the synthetic new way that obtains novel cabbage type rape, do not appear in the newspapers as yet.
Summary of the invention
Defective in view of above prior art existence, in order to obtain new cabbage type rape route of synthesis, enrich the cabbage type rape genetic resource, promote the research of cabbage type rape genetic breeding, the object of the present invention is to provide a kind of method of artificially developing novel brassica napus, the technology path of this method is to organically combine by biotechnology and normal experiment means, obtains novel cabbage type rape.
The method of the artificial developing novel brassica napus of the present invention, realize successively through the following steps:
(1) wild cabbage and mustard type rape being carried out interspecific cross, is that 10% NaCl salt solution is smeared column cap+bud pollination with mass percent concentration, obtains hybrid S by rataria redemption technology
0, and set up hybridal clone colony, S
0Genotype is 2n=3x=ABC=27;
(2) with hybrid S
0Carry out selfing, flower bud phase supplementary pollination overcomes its self-incompatibillity by rataria redemption technology and obtains self progeny S
1, and set up its clone colony;
(3) step (2) rataria is saved the clone colony that obtains and transplanted the land for growing field crops, carry out selfing, flower bud phase supplementary pollination obtains self progeny S
2
(4) with the S that obtains
2Carry out selfing, obtain S
3To S
3Carry out selfing and obtain S
4From the S that obtains
4Begin to carry out morphology and cytological Identification, at advanced lines material S
4Or S
5The middle novel cabbage type rape of seed selection.
In the said method step, wild cabbage is kale, head cabbage or cabbage mustard etc.
In the said method step, rataria saves technology, set up correlation technique such as clone colony is prior art, will describe in further detail the inventive method in the embodiment of back.
Description of drawings
Fig. 1 is the method flow diagram of artificially developing novel brassica napus.
Advantage of the present invention is:
The cross compatibility that carries out interspecific hybridization with mustard type rape and wild cabbage is higher than the cross compatibility of Chinese cabbage and wild cabbage, easily obtains Get species hybrid; Simultaneously, this approach synthesizing new cabbage type rape and artificial directly synthetic sweet with Chinese cabbage and brassica specie intermolecular hybrid Blue type rape is compared, and has replaced turnip type rape with mustard type rape, can be with mustard type rape drought resisting, cold-resistant, and plant type is excellent The advantage such as good imports in the cabbage type rape, and its inheritance stability speed is fast, and the character variation scope is big, chooses easily merit Cabbage type rape. In addition, the cabbage type rape AC genome chromosome of this approach acquisition comes from respectively juncea oil Dish and wild cabbage differ greatly with existing cabbage type rape hereditary basis, more is conducive to the Wild cabbage type inter-subgenomic heterosis Utilize, improve the rape level of production.
Embodiment
Below in conjunction with specific embodiment method provided by the present invention is described; described embodiment is in order to explain the present invention better; rather than to the restriction of claim protection domain of the present invention, all belong to the present invention and ask for protection scope based on the modification and the change of basic thought of the present invention.
Embodiment 1:
When the 2-4 month, rape was in full-bloom stage, the mustard type rape of choosing robust growth is for maternal, extract and bloomed and too small bud, nearly 1-2d is castrated the bud of opening, with mass percent concentration is that 10% NaCl smears column cap, Deng awarding the pollen of being got with from the healthy and strong plant of kale behind the salt solution natural air drying, the paper bag bagging is used rapidly in the pollination back, prevents that foreign pollen from importing into.Repeated pollination in second day.
About the 20d of pollination back, get the tender ovule of immature children, under aseptic condition with after 75% the alcohol surface sterilization, be clorox sterilization 15-20 minute of 10%-15% again with mass percent concentration, be inoculated into then on the MS medium, after treating its sprouting, be transferred on the fast breeding culture medium and breed fast, obtain clone.10-11 was transferred to clone in the MS cultivation about month, treated its transplanting land for growing field crops, back of taking root, and carried out precision management.
About in March, second, to be identifiedly to choose plant and force selfing (artificial supplementary pollination) with a small amount of pollen for after the clone of true hybrid blooms, pollination back about 15-20 days is got its ovule and is carried out rataria and save (operating procedure is seen epimere), obtains S
1The clone of colony.
To S
1The colony plant still adopts the mode of artificial supplementary pollination to carry out selfing, obtains S
2Continue later on the offspring who obtains is carried out selfing, its colony is along with the increase of selfing algebraically, and its self-fruitful rate increases substantially.To S
4The time, its self-fruitful rate is tending towards normally, no longer needs to carry out supplementary pollination.
From S
4Rise, the material that obtains is carried out cytology, morphologic evaluation and selection, select chromosome number and level off to 38, form levels off to the plant of cabbage type rape and carries out genetic breeding research.
Embodiment 2: wild cabbage is selected head cabbage for use, carries out above-mentioned example operation simultaneously.
Embodiment 3: wild cabbage is selected cabbage mustard for use, carries out above-mentioned example operation simultaneously.
Claims (2)
1, a kind of method of artificially developing novel brassica napus is characterized in that realizing through the following steps successively:
(1) wild cabbage and mustard type rape being carried out interspecific cross, is that 10% NaCl salt solution is smeared column cap+bud pollination with mass percent concentration, obtains hybrid S by rataria redemption technology
0, and set up hybridal clone colony, S
0Genotype is 2n=3x=ABC=27;
(2) with hybrid S
0Carry out selfing, flower bud phase supplementary pollination overcomes its self-incompatibillity by rataria redemption technology and obtains self progeny S
1, and set up its clone colony;
(3) step (2) rataria is saved the clone colony that obtains and transplanted the land for growing field crops, carry out selfing, flower bud phase supplementary pollination obtains self progeny S
2
(4) with the S that obtains
2Carry out selfing, obtain S
3To S
3Carry out selfing and obtain S
4Begin to carry out morphology and cytological Identification from the Sx that obtains, at advanced lines material S
4Or S
5The middle novel cabbage type rape of seed selection.
2, method according to claim 1 is characterized in that, wild cabbage is kale, head cabbage or cabbage mustard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910103551XA CN101513168B (en) | 2009-04-07 | 2009-04-07 | Method for artificially developing novel brassica napus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910103551XA CN101513168B (en) | 2009-04-07 | 2009-04-07 | Method for artificially developing novel brassica napus |
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| Publication Number | Publication Date |
|---|---|
| CN101513168A true CN101513168A (en) | 2009-08-26 |
| CN101513168B CN101513168B (en) | 2011-10-05 |
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ID=41037916
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910103551XA Expired - Fee Related CN101513168B (en) | 2009-04-07 | 2009-04-07 | Method for artificially developing novel brassica napus |
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| CN (1) | CN101513168B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871803A (en) * | 2015-06-18 | 2015-09-02 | 云南农业大学 | Method for improving seeding performance of erigeron breviscapus |
| CN105981645A (en) * | 2015-12-22 | 2016-10-05 | 江西农业大学 | Efficient breeding method for early-maturing, high-yield and multi-resistant Brassica campestris L. |
| CN109168421A (en) * | 2018-10-09 | 2019-01-11 | 江西省农业科学院作物研究所 | Application of the brassicacarinata in the breeding of cabbage type rape definite inflorescence strain |
| CN109548644A (en) * | 2019-01-07 | 2019-04-02 | 西南大学 | A method of widening cabbage type rape hereditary variation range |
| CN109906937A (en) * | 2019-01-28 | 2019-06-21 | 湖北大学 | A kind of breeding method of cabbage type rape drought resisting germplasm |
| CN110506623A (en) * | 2019-09-17 | 2019-11-29 | 云南省农业科学院园艺作物研究所 | A kind of method of Cruciferae interspecific hybridization Setting percentage |
| CN113854147A (en) * | 2021-09-26 | 2021-12-31 | 贵州省油菜研究所 | Method for cultivating excellent germplasm resources of rapes |
-
2009
- 2009-04-07 CN CN200910103551XA patent/CN101513168B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871803A (en) * | 2015-06-18 | 2015-09-02 | 云南农业大学 | Method for improving seeding performance of erigeron breviscapus |
| CN105981645A (en) * | 2015-12-22 | 2016-10-05 | 江西农业大学 | Efficient breeding method for early-maturing, high-yield and multi-resistant Brassica campestris L. |
| CN109168421A (en) * | 2018-10-09 | 2019-01-11 | 江西省农业科学院作物研究所 | Application of the brassicacarinata in the breeding of cabbage type rape definite inflorescence strain |
| CN109168421B (en) * | 2018-10-09 | 2021-06-08 | 江西省农业科学院作物研究所 | Application of Arabidopsis thaliana in breeding of brassica napus limited inflorescence strain |
| CN109548644A (en) * | 2019-01-07 | 2019-04-02 | 西南大学 | A method of widening cabbage type rape hereditary variation range |
| CN109906937A (en) * | 2019-01-28 | 2019-06-21 | 湖北大学 | A kind of breeding method of cabbage type rape drought resisting germplasm |
| CN110506623A (en) * | 2019-09-17 | 2019-11-29 | 云南省农业科学院园艺作物研究所 | A kind of method of Cruciferae interspecific hybridization Setting percentage |
| CN113854147A (en) * | 2021-09-26 | 2021-12-31 | 贵州省油菜研究所 | Method for cultivating excellent germplasm resources of rapes |
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| Publication number | Publication date |
|---|---|
| CN101513168B (en) | 2011-10-05 |
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Granted publication date: 20111005 Termination date: 20140407 |