CN109156300A - A kind of breeding of resistance to bolting radish is reserved seed for planting method - Google Patents

A kind of breeding of resistance to bolting radish is reserved seed for planting method Download PDF

Info

Publication number
CN109156300A
CN109156300A CN201811317377.4A CN201811317377A CN109156300A CN 109156300 A CN109156300 A CN 109156300A CN 201811317377 A CN201811317377 A CN 201811317377A CN 109156300 A CN109156300 A CN 109156300A
Authority
CN
China
Prior art keywords
radish
bolting
resistance
seed
planting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201811317377.4A
Other languages
Chinese (zh)
Other versions
CN109156300B (en
Inventor
宋正旭
周英
马荣群
张宏斌
江志训
马丽琨
李磊
王殿纯
赵征宇
孙吉禄
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
QINGDAO INSTITUTE OF AGRICULTURAL SCIENCES
Original Assignee
QINGDAO INSTITUTE OF AGRICULTURAL SCIENCES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by QINGDAO INSTITUTE OF AGRICULTURAL SCIENCES filed Critical QINGDAO INSTITUTE OF AGRICULTURAL SCIENCES
Priority to CN201811317377.4A priority Critical patent/CN109156300B/en
Publication of CN109156300A publication Critical patent/CN109156300A/en
Application granted granted Critical
Publication of CN109156300B publication Critical patent/CN109156300B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

It reserves seed for planting method the present invention relates to resistance to bolting radish varieties election effects field more particularly to a kind of breeding of resistance to bolting radish.Include the steps that sowing, refrigerate management, seed collecting after pre-treatment, refrigeration, transplanting pre-treatment, transplanting, cultivation, the present invention is by selecting the good single plant of commodity and bolting resistant property to refrigerate after sowing the seed in spring, seed after autumn, plantation was collected seed is resistance to bolting radish, the commodity and bolting resistant property of radish can be selected simultaneously, the plantation of resistance to bolting radish can be completed in 1 year again, selects and reserves seed for planting, efficiency of selection is high.

Description

A kind of breeding of resistance to bolting radish is reserved seed for planting method
Technical field
The present invention relates to resistance to bolting radish varieties election effects field more particularly to a kind of breeding sides of reserving seed for planting of resistance to bolting radish Method.
Background technique
Radish (scientific name: Raphanus sativus L.) Cruciferae Rhaphanus 2 years or annual herb plant, high 20- 100 centimetres, taproot meat, oblong, spherical shape or cone, crust green, white or red, stem has branch, hairless, slightly has a powder Frost.Racemes terminal and axillary, grey color or pink, carpopodium is 1-1.5 centimetres long, the month at florescence 4-5, the fruiting period 5-6 month.
Radish sowing time or cultivation management not at that time easily in earlier bolting, such as production if variety selection and management not When sowing time arranges unreasonable, it is easy to which bolting is bloomed before product organ is formed or in forming process.Radish occurs earlier After bolting, nutrient manufactured by assimilation largely supplies flower a kind of sedge growth, and fleshy root stopping is expanded or subalimentation, to go out Show dehydration and cause the chaff heart, leads to radish quality decline, or even lose edible value.So the radish varieties of the resistance to bolting of breeding for The plantation of radish spring is of great significance, and it is a difficult point of resistance to bolting radish varieties selection that resistance to bolting radish, which is reserved seed for planting,.
Existing resistance to bolting radish method of reserving seed for planting mainly has " seed low-temperature treatment method " and " winter brooder seeding method "." seed is low Warm facture " method is simple, readily selected radish bolting resistant property, but cannot be selected according to radish commodity;" winter brooder seeding method " It can choose bolting resistant property, can also be selected according to part radish commodity, but bolting resistant property and commodity selection and spring open country Kind is implanted with different.
Summary of the invention
The technical problem to be solved by the present invention is existing resistance to bolting radish is reserved seed for planting in method, " seed low-temperature treatment method " side Method is simple, readily selected radish bolting resistant property, but cannot be selected according to radish commodity;" winter brooder seeding method " can choose resistance to pumping A kind of sedge can also be selected according to part radish commodity, but bolting resistant property and commodity selection with spring open country kind be implanted with centainly it is poor It is different.
The method in order to solve the above technical problems, breeding that the present invention provides a kind of resistance to bolting radish is reserved seed for planting, by sowing the seed in spring The good single plant refrigeration of commodity and bolting resistant property is selected afterwards, and the seed after autumn, plantation was collected seed is resistance to bolting radish, Neng Goutong When the commodity and bolting resistant property of radish are selected, and the plantation of resistance to bolting radish can be completed in 1 year, select and stay Kind, efficiency of selection is high.
In order to achieve the above objectives, it reserves seed for planting method the invention discloses a kind of breeding of resistance to bolting radish, by following steps group At:
(1) sow: resistance to bolting radish is planted in mid or late March open country in In The Area of Qingdao, investigation selection commodity at the beginning of 6 months by the end of May With the good single plant of bolting resistant property;Be selected in and sow the seed in spring, with spring open country planting environment select be suitble to spring sowing kind and The good single plant of bolting resistant property carries out storage and reserves seed for planting;
(2) it refrigerates pre-treatment: an evening will be placed in the room to cool after harvest in the radish afternoon chosen;The next morning Radish is individually wrapped with newspaper, is cased;Placing an evening can be appropriate to reduce radish water content with Temperature fall, is conducive to radish storage Hiding.
(3) it refrigerates: radish being taken out out of freezer every 1 month, is dried in the air in the cool 1-2 hours, until radish surface moisture is dried in the air It is dry to be advisable.It generally dries in the air 1 time in the entire refrigeration phase;Low temperature can reduce radish respiration consumption be conducive to storage, while radish to bloom need Radish can be promoted preferably to pass through vernalization by vernalization, cryopreservation, the bolting flowering time after shortening radish transplanting.
(4) pre-treatment: transplanting mid-August is transplanted;1.5 meters of flat furrow are done, the last week filling flood is planted and makes entropy, soil when making to plant radish Earth hand pinches agglomerating be advisable;Soil moisture is suitable for that can promote radish Rapid Rooting.If directly watering after planting, because soil moisture is big Radish is easy to rot;It does not water, the inadequate radish of soil moisture is not easy to take root, and last dehydration air-dries.Shift to an earlier date one in test in 2018 It is respectively 86% and 24% that week watering, which makes entropy and do not make the survival rate of entropy,.
(5) it transplants: opening furrow in advance: every two row of ridge cultivation, spacing in the rows 40cm;Trench digging, radish root 1/3 are buried in soil, and light pressure makes trailing plants Foretell root to combine closely with soil;Shallow ridges, which is opened, in radish two sides later forms micro-ridge;Radish root 1/3, which is buried, can guarantee radish in soil Root system comes into full contact with soil, does not influence to radiate, and moisture loss is few, is conducive to radish and survives.It has some setbacks greater than 1/3 radish respiratory heat loss Freely, radish is easy to cause to rot;Soil cannot be come into full contact with greater than 1/3 radish root system, dehydration is easy to cause to air-dry.In radish two sides It opens shallow ridges and forms micro-ridge convenient for watering.
(6) it is managed after planting: covering shading net, light transmittance 60-70% after cultivation outside arched shed;Early autumn solar irradiation is strong, sunlight Direct projection easily leads to radish dehydration and air-dries, and shades it is possible to prevente effectively from such case.
(7) it collects seed: collecting seed and continue to employ after radish produces seeds.
Further, chest can use carton or wooden case in step (2);One layer of mulch is first spread in the case, for radish packet Firmly, then the radish wrapped with newspaper is put into chest, mulch is sealed after piling, chest is sealed.Mulch main function is to protect Radish moisture content is held, newspaper main function is to draw the moisture that surface generates in radish radish storage, and holding radish surface as far as possible is dry It is dry.
Further, the condition of step (3) refrigeration is 1 DEG C ± 1 DEG C of temperature, humidity 90%-100%.In this temperature and Stored refrigerated hardly possible can be overcome the problems, such as under humidity.Temperature lower than 0 DEG C of radish can frostbite, when transplanting is perishable to be not easy to survive;Such as Shown in the following table 1, temperature is higher than 2 DEG C of radish meeting nature in storage and takes root, and consumes radish nutrient itself, nutrient is transplanted after scattering and disappearing Radish survival rate declines afterwards, and with temperature raising radish, the amount of taking root is obviously improved naturally within the scope of 2 DEG C -10 DEG C, and nutrient consumption increases, Survival rate is lower;As shown in table 2, the too low radish dehydration of humidity air-dries, and vigor decline, survival rate is low, and humidity is excessively high, and radish is perishable Rotten, radish water content is generally in 91%-97%, therefore storage humidity is maintained at 90%-100% and is conducive to radish internal water guarantor It holds.
Influence of the different storage temperature Temperature Treatments of table 1 to radish transplanting survival rate
Treatment temperature Survival rate
1℃±1℃ 96%
5℃±1℃ 85%
8℃±1℃ 50%
Note: being 95% using storage humidity
The different storage humidity of table 2 handle the influence to radish transplanting survival rate
Handle humidity Survival rate
100% 82%
95% 85%
50% 40%
30% 28%
Note: being 5 DEG C ± 1 DEG C using storage temperature
Further, step (6) middle shed will keep divulging information;Every morning supplements amount of water to radish with sprayer, directly Start to send out young leaves to radish;Hereafter shading net is removed, by normal management.Initial stage after radish transplanting not yet sends out new root, resists Power is poor, humidity is excessive easily lead to it is rotten, it is too small to easily lead to dehydration, and early autumn temperature of shed is high.By divulging information, moisturizing can be fitted When control field air humidity and temperature, the condition for being conducive to take root is created, improves transplanting survival rate.
The beneficial effects of the present invention are:
(1) commodity and bolting resistant property of radish can be selected simultaneously, the method by directly sowing the seed in spring is used True environmental selection is suitble to the kind of practical spring open country plantation.
(2) spring after planting selects autumn after the good single plant of commodity and bolting resistant property, refrigeration to transplant, and then reserves seed for planting, The plantation of resistance to bolting radish is completed in year, selects and reserves seed for planting, and work efficiency is high.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with implementation of the invention Example, technical scheme in the embodiment of the invention is clearly and completely described.Obviously, described embodiment is the present invention A part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
Embodiment 1:
A kind of breeding of resistance to bolting radish is reserved seed for planting method, is comprised the steps of:
(1) sow: resistance to bolting radish is planted in mid or late March open country in In The Area of Qingdao, investigation selection commodity at the beginning of 6 months by the end of May With the good single plant of bolting resistant property;Be selected in and sow the seed in spring, with spring open country planting environment select be suitble to spring sowing kind and The good single plant of bolting resistant property carries out storage and reserves seed for planting;
(2) it refrigerates pre-treatment: an evening will be placed in the room to cool after harvest in the radish afternoon chosen;The next morning Radish is individually wrapped with newspaper, is cased;Placing an evening can be appropriate to reduce radish water content with Temperature fall, is conducive to radish storage Hiding.Wherein, chest can use carton or wooden case;Then newspaper packet will be used for radish to be encased by first spreading one layer of mulch in the case Good radish is put into chest, mulch is sealed after piling, chest is sealed.Mulch main function is to maintain radish moisture content, newspaper master Acting on is to draw the moisture that surface generates in radish radish storage, as far as possible holding radish dry tack free.
(3) it refrigerates: radish being taken out out of freezer every 1 month, is dried in the air in the cool 1-2 hours, until radish surface moisture is dried in the air It is dry to be advisable.It generally dries in the air 1 time in the entire refrigeration phase;Low temperature can reduce radish respiration consumption be conducive to storage, while radish to bloom need Radish can be promoted preferably to pass through vernalization by vernalization, cryopreservation, the bolting flowering time after shortening radish transplanting.
(4) pre-treatment: transplanting mid-August is transplanted;1.5 meters of flat furrow are done, the last week filling flood is planted and makes entropy, soil when making to plant radish Earth hand pinches agglomerating be advisable;Soil moisture is suitable for that can promote radish Rapid Rooting.If directly watering after planting, because soil moisture is big Radish is easy to rot;It does not water, the inadequate radish of soil moisture is not easy to take root, and last dehydration air-dries.In test in 20 18 years in advance It is respectively 86% and 24% that watering in one week, which makes entropy and do not make the survival rate of entropy,.
(5) it transplants: opening furrow in advance: every two row of ridge cultivation, spacing in the rows 40cm;Trench digging, radish root 1/3 are buried in soil, and light pressure makes trailing plants Foretell root to combine closely with soil;Shallow ridges, which is opened, in radish two sides later forms micro-ridge;Radish root 1/3, which is buried, can guarantee radish in soil Root system comes into full contact with soil, does not influence to radiate, and moisture loss is few, is conducive to radish and survives.It has some setbacks greater than 1/3 radish respiratory heat loss Freely, radish is easy to cause to rot;Soil cannot be come into full contact with greater than 1/3 radish root system, dehydration is easy to cause to air-dry.In radish two sides It opens shallow ridges and forms micro-ridge convenient for watering.
(6) it is managed after planting: covering shading net, light transmittance 60-70% after cultivation outside arched shed;Early autumn solar irradiation is strong, sunlight Direct projection easily leads to radish dehydration and air-dries, and shades it is possible to prevente effectively from such case.
(7) it collects seed: collecting seed and continue to employ after radish produces seeds.
Embodiment 2:
Wherein, the condition of step (3) refrigeration is 1 DEG C ± 1 DEG C of temperature, humidity 90%-100%.In this temperature and humidity Stored refrigerated hardly possible can be overcome the problems, such as down.Temperature lower than 0 DEG C of radish can frostbite, when transplanting is perishable to be not easy to survive;Such as following table Shown in 1, temperature is higher than 2 DEG C of radish meeting nature in storage and takes root, and consumes radish nutrient itself, trailing plants after nutrient is transplanted after scattering and disappearing Foretell survival rate decline, the amount of taking root is obviously improved naturally with temperature raising radish within the scope of 2 DEG C -10 DEG C, and nutrient consumption increases, and survives Rate is lower;As shown in table 2, the too low radish dehydration of humidity air-dries, and vigor decline, survival rate is low, and humidity is excessively high, and radish is perishable, radish Water content is generally in 91%-97%, therefore storage humidity is maintained at 90%-100% and is conducive to the holding of radish internal water.
Remaining is same as Example 1.
Embodiment 3:
Wherein, step (6) middle shed will keep divulging information;Every morning supplements amount of water to radish with sprayer, until trailing plants Fore-telling, which is opened, originates young leaves;Hereafter shading net is removed, by normal management.Initial stage after radish transplanting not yet sends out new root, resistance compared with Difference, humidity is excessive easily lead to it is rotten, it is too small to easily lead to dehydration, and early autumn temperature of shed is high.By divulging information, moisturizing can be controlled suitably Field air humidity and temperature processed create the condition for being conducive to take root, and improve transplanting survival rate.
Remaining is identical as embodiment 1 or embodiment 2.

Claims (4)

  1. A kind of method 1. breeding of resistance to bolting radish is reserved seed for planting, it is characterised in that comprise the steps of:
    (1) sow: resistance to bolting radish is planted in mid or late March open country in In The Area of Qingdao, investigation selection commodity at the beginning of 6 months by the end of May and resistance to The good single plant of bolting;
    (2) it refrigerates pre-treatment: an evening will be placed in the room to cool after harvest in the radish afternoon chosen;The next morning is by radish It is individually wrapped, is cased with newspaper;
    (3) refrigerate: radish is taken out out of freezer every 1 month, is dried in the air in the cool 1-2 hours, until radish surface moisture dry for Preferably.It generally dries in the air 1 time in the entire refrigeration phase;
    (4) pre-treatment: transplanting mid-August is transplanted;1.2 meters of flat furrow are done, the last week filling flood is planted and makes entropy, soil hand when making to plant radish Pinch agglomerating be advisable;
    (5) it transplants: opening furrow in advance: every two row of ridge cultivation, spacing in the rows 40cm;Trench digging, radish root 1/3 are buried in soil, and light pressure makes radish root It combines closely with soil in portion;Shallow ridges, which is opened, in radish two sides later forms micro-ridge;
    (6) it is managed after planting: covering shading net, light transmittance 60-70% after cultivation outside arched shed;
    (7) it collects seed: collecting seed and continue to employ after radish produces seeds.
  2. The method 2. breeding of resistance to bolting radish as described in claim 1 is reserved seed for planting, it is characterised in that: chest carton in step (2) Or wooden case;One layer of mulch is first spread in the case then the radish wrapped with newspaper is put into chest, piles for encasing radish Mulch is sealed afterwards, chest is sealed.
  3. The method 3. breeding of resistance to bolting radish as described in claim 1 is reserved seed for planting, it is characterised in that: the condition of step (3) refrigeration is 1 DEG C ± 1 DEG C of temperature, humidity 90-100%.
  4. The method 4. breeding of resistance to bolting radish as described in claim 1 is reserved seed for planting, it is characterised in that: step (6) middle shed will be kept Ventilation;Every morning supplements amount of water to radish with sprayer, until radish starts to send out young leaves;Hereafter shading net is removed, by just Often management.
CN201811317377.4A 2018-11-07 2018-11-07 Seed reserving method for breeding bolting-resistant radish Active CN109156300B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811317377.4A CN109156300B (en) 2018-11-07 2018-11-07 Seed reserving method for breeding bolting-resistant radish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811317377.4A CN109156300B (en) 2018-11-07 2018-11-07 Seed reserving method for breeding bolting-resistant radish

Publications (2)

Publication Number Publication Date
CN109156300A true CN109156300A (en) 2019-01-08
CN109156300B CN109156300B (en) 2021-02-12

Family

ID=64876644

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811317377.4A Active CN109156300B (en) 2018-11-07 2018-11-07 Seed reserving method for breeding bolting-resistant radish

Country Status (1)

Country Link
CN (1) CN109156300B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110583407A (en) * 2019-10-18 2019-12-20 青岛市农业科学研究院 Radish seed-replacing and reserving method
CN112586131A (en) * 2020-12-14 2021-04-02 奉节县承青蔬菜种植专业合作社 Radish seed cultivation method

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1248396A (en) * 1999-09-08 2000-03-29 河南省民权县蔬菜研究所 Breeding method for radish model Deri 2
CN1788543A (en) * 2004-12-14 2006-06-21 东北农业大学 Bolting-tolerant export special type cabbage variety breeding method
US20080034450A1 (en) * 2006-08-04 2008-02-07 Frampton Anna J Integration of commercial plant breeding and genomic technologies
CN101755597A (en) * 2010-01-18 2010-06-30 东北农业大学 Method for inducing potato onion to bolt and flower
CN102282997A (en) * 2011-06-27 2011-12-21 马文浩 Planting, seed selection and edible making method for Jinliansi radish
CN102311489A (en) * 2010-07-08 2012-01-11 中国科学院上海生命科学研究院 Plant heat-resistant gene BccDREB2A and application thereof
CN103947539A (en) * 2014-04-26 2014-07-30 民权县民权种业有限公司 Breeding method for minquan-series radish
CN104160870A (en) * 2014-09-03 2014-11-26 镇江瑞繁农艺有限公司 Curd type broccoli cultivation method

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1248396A (en) * 1999-09-08 2000-03-29 河南省民权县蔬菜研究所 Breeding method for radish model Deri 2
CN1788543A (en) * 2004-12-14 2006-06-21 东北农业大学 Bolting-tolerant export special type cabbage variety breeding method
US20080034450A1 (en) * 2006-08-04 2008-02-07 Frampton Anna J Integration of commercial plant breeding and genomic technologies
CN101755597A (en) * 2010-01-18 2010-06-30 东北农业大学 Method for inducing potato onion to bolt and flower
CN102311489A (en) * 2010-07-08 2012-01-11 中国科学院上海生命科学研究院 Plant heat-resistant gene BccDREB2A and application thereof
CN102282997A (en) * 2011-06-27 2011-12-21 马文浩 Planting, seed selection and edible making method for Jinliansi radish
CN103947539A (en) * 2014-04-26 2014-07-30 民权县民权种业有限公司 Breeding method for minquan-series radish
CN104160870A (en) * 2014-09-03 2014-11-26 镇江瑞繁农艺有限公司 Curd type broccoli cultivation method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110583407A (en) * 2019-10-18 2019-12-20 青岛市农业科学研究院 Radish seed-replacing and reserving method
CN112586131A (en) * 2020-12-14 2021-04-02 奉节县承青蔬菜种植专业合作社 Radish seed cultivation method

Also Published As

Publication number Publication date
CN109156300B (en) 2021-02-12

Similar Documents

Publication Publication Date Title
CN103070001B (en) Method for safely planting okra at high yield and high quality
CN101971757B (en) Cultivation method for oriental lily fresh cut-flower
CN104920051B (en) One plants vegetables the method kept a full stand of seedings with listing in advance with type sweet potato winter greenhouse
CN109699407B (en) Facility-dedicated small-fruit watermelon non-specific area early-maturing promoting cultivation technology
CN104303774B (en) A kind of cultivation method of famous sun-cured tobaccos
CN104145688A (en) Cultivation method of broccoli
CN104221630B (en) Summer squash vernalization and efficient cultivation method
CN107432225A (en) One seed pod mulberry form culture method
CN106171333A (en) The implantation methods of Chinese cabbage
CN103348850A (en) Cultivation method of Chinese chives
CN110506620A (en) A kind of greenhouse vegetable rock wool recycling method based on crop rotation
CN109328815A (en) A kind of implantation methods of peach
CN109566404B (en) Method for producing hybrid seeds of two-season loofah in one year
CN109156300A (en) A kind of breeding of resistance to bolting radish is reserved seed for planting method
CN112219651B (en) Double-cropping and double-cropping cultivation method for greenhouse watermelons in Jiangsu coastal areas
CN105453877A (en) Four-crop-annual interplanting cultivation method
CN105265193B (en) Cauliflower grafting is reserved seed for planting method
CN105519316A (en) Asparagus lettuce cultivation method
Undersander et al. Alfalfa germination & growth
CN109121549A (en) A method of rhizoma polygonati is improved without bud rhizome germination rate
CN105284590B (en) The breeding method of precocity shape sponge gourd with a tight waist
CN104641880A (en) Method for planting dendrobium candidum in camphor tree forest
CN105706715B (en) Willow leaf cauliflower is avoided the peak hour the method for breeding
CN107494252A (en) A kind of method for improving eggplant hybrid seeding efficiency
CN109662000B (en) Cultivation method for improving seed production yield of tuber mustard

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant