CN109121552B - Method for physically breaking dormancy of hovenia dulcis thunb seeds - Google Patents
Method for physically breaking dormancy of hovenia dulcis thunb seeds Download PDFInfo
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- CN109121552B CN109121552B CN201811077141.8A CN201811077141A CN109121552B CN 109121552 B CN109121552 B CN 109121552B CN 201811077141 A CN201811077141 A CN 201811077141A CN 109121552 B CN109121552 B CN 109121552B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a method for physically breaking the dormancy of hovenia dulcis thunb seeds, which comprises the following steps: a. kneading the hovenia dulcis thunb fruits with dry peels; b. b, disinfecting the seeds obtained in the step a by using a potassium permanganate solution at normal temperature, cleaning by using clear water, soaking the seeds for 30min by using warm water at the temperature of 40-50 ℃, and then soaking the seeds for 1-2d by using the clear water at normal temperature; c. selecting seeds with obvious imbibition, stabilizing the seeds with a pair of tweezers to ensure that the seeds are 0.15-0.25cm higher than the tweezers, and cutting downwards by using a blade in a direction vertical to a ridge line or cutting off the endosperm along a direction parallel to the ridge line to expose. d. Paving absorbent cotton on the bottom layer of a culture vessel, wetting the absorbent cotton with clear water, and placing seeds with broken seed shells in the culture vessel; and putting the culture vessel into a light incubator for culture. Short germination time and stable effect. The germination time of the hovenia dulcis thunb seeds is about 3-4 days, the germination rate reaches 82.3%, and the germination rate is stable.
Description
Technical Field
The invention relates to the technical field of forestry seedling cultivation. More specifically, the invention relates to a method for promoting the germination of hovenia dulcis thunb seeds.
Background
Hovenia dulcis (Hovenia acerba Lindl.) belongs to Rhamnaceae (Rhamnaceae) Hovenia genus, is distributed in China, Japan, India and Korea, and is a valuable precious tree species integrating fruit, medicine and material: the fleshy fruit stalks contain various sugars, vitamins and amino acids essential to human bodies, can also be used as raw fruits and have high nutritional value; the fruits, seeds, leaves and roots of the Chinese medicinal composition can be used as medicaments, and have high medical value; the wood material is hard, the texture is beautiful and easy to process, and the wood material is a good building decoration material.
Because the hovenia dulcis thunb seeds have dormancy, the germination rate under the natural condition is only 12% -20%, the seedlings are irregular, the duration is long, and the efficient development and utilization of the tree seeds are hindered. Therefore, technical researches for breaking the dormancy mechanism of hovenia dulcis thunb seeds and improving the germination rate of the seeds are urgently needed.
Nanjing university of forestry college newspaper (Nature science edition). 2014(2) discloses a reason and a releasing method for the dormancy of hovenia dulcis seeds. In the document, germination is started 4 days after the part of seed coats at the non-radicle part is cut off and put in a bed, the germination rate is 80%, and germination is started 6 days after the surface of the abraded seeds is put in the bed, and the germination rate is 73%.
In the implementation process of the existing hovenia acerba sowing and seedling raising technology, the following defects are found:
1. the dried hovenia dulcis thunb seed shell is hard, the seed embryo structure is easily damaged in the seed coat cutting process, the cutting failure is caused, the success rate is low, and time and labor are wasted;
2. in the process of rubbing seeds with abrasive paper, much time is consumed, the effect of uniformly scratching the seeds cannot be achieved, and the scratching degree of a part of the seeds is relatively low, so that the germination rate of the seeds is very low and cannot be expected generally.
3. The existing method does not guarantee the short germination time of the hovenia dulcis thunb seeds, the time-saving and labor-saving seed treatment mode and the higher seed germination rate at the same time.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the damage of the hovenia acerba seed coat is time-consuming and labor-consuming, the success rate is low, the seed germination rate cannot be ensured, and the like.
The technical scheme of the invention is as follows: a method for physically breaking the dormancy of hovenia dulcis thunb seeds comprises the following steps:
a. kneading the raisin tree fruit with dried peel, immersing the peeled seeds in water, removing the upper floating debris and seeds, and keeping the lower submerged seeds;
b. b, disinfecting the seeds obtained in the step a by using a potassium permanganate solution at normal temperature, cleaning by using clear water, soaking the seeds for 25-35min by using warm water at 40-50 ℃, and then soaking the seeds for 1-2d by using the clear water at normal temperature;
c. selecting seeds with obvious imbibition, stabilizing the seeds with a pair of tweezers to enable the seeds to be higher than the tweezers by 0.15-0.25cm, and cutting downwards in a direction vertical to a seed ridge line or in a direction parallel to the seed ridge line by using a blade to enable endosperm to be exposed.
d. Paving absorbent cotton on the bottom layer of a culture vessel, wetting the absorbent cotton with clear water, and placing seeds with broken seed shells in the culture vessel; and putting the culture vessel into a light incubator for culture.
Further, in the step b, the concentration of the potassium permanganate solution is 0.3-0.5g/L, and the disinfection time is 10 min.
Further, the incubator conditions were: the illumination time and the non-illumination time are both 12h, the temperature is set to be 20 ℃, the air humidity is set to be 70%, the illumination intensity is 1155lx light intensity, ventilation is always kept, and the moisture content of the culture medium is checked at regular time.
By adopting the method, the speed of damaging the seed coat of the hovenia dulcis thunb seed is 30-60 granules/min per person, the seed coat damage success rate is 98%, the seed embryo without the seed coat is intact, the white part begins to appear on the 3 rd-4 th day after the seed germination is accelerated, the full period of the seed germination is 9 th-14 th day, and the germination rate is 76.3%.
Compared with the prior art, the invention has the following beneficial effects:
1. the operation is simple. The invention only needs to cut the seed ridge line to achieve the purpose that the seed coat has cracks, has no influence on the seed embryo, and saves time and labor when cutting the seed coat.
2. Short germination time and stable effect. The germination time of the hovenia dulcis thunb seeds is about 3-4 days, the germination rate reaches 81.1-82.3%, and the germination rate is stable.
Detailed Description
Example 1
a. Kneading the raisin tree fruit with dried peel, immersing the peeled seeds in water, separating the submerged seeds from the floating seeds, removing the floating debris and seeds from the upper layer with a tool, and retaining the seeds at the lower layer.
b. And (b) disinfecting the seeds obtained in the step (a) by using a potassium permanganate solution at normal temperature, wherein the concentration of the potassium permanganate solution is 0.3g/L, and the disinfection time is 8 min. Then cleaning with clear water, soaking the seeds in warm water of 40 ℃ for 35min, and then soaking the seeds in clear water at normal temperature for 2 d.
c. Selecting seeds with obvious imbibition, stabilizing the seeds with tweezers to enable the seeds to be higher than the tweezers by 0.25cm, and cutting downwards by using a blade in a direction vertical to a seed ridge line to enable endosperm to be exposed.
d. Using absorbent cotton with the thickness of about 0.7cm as a culture medium to be paved on the bottom layer of a culture vessel, wetting the absorbent cotton by using clear water, and placing the seeds with the broken seed shells in the culture vessel; putting the culture vessel into an illumination incubator for culture, wherein the conditions of the incubator are as follows: the illumination time and the non-illumination time are both 12h, the temperature is set to be 20 ℃, the air humidity is set to be 70%, the illumination intensity is 1155lx light intensity, ventilation is always kept, and the moisture content of the culture medium is checked at regular time.
Example 2
a. Kneading the raisin tree fruit with dried peel, immersing the peeled seeds in water, removing the upper floating debris and seeds, and keeping the lower submerged seeds;
b. and (b) disinfecting the seeds obtained in the step (a) by using a potassium permanganate solution at normal temperature, wherein the concentration of the potassium permanganate solution is 0.5g/L, and the disinfection time is 12 min. Then cleaning the seeds with clear water, soaking the seeds in warm water at 50 ℃ for 25min, and then soaking the seeds in clear water for 1d at normal temperature;
c. selecting seeds with obvious imbibition, stabilizing the seeds with a pair of tweezers, keeping the seeds higher than the tweezers by 0.15cm, and using a blade to cut the seeds in a direction parallel to the ridge line to expose endosperm.
d. Using absorbent cotton with the thickness of about 1cm as a culture medium to be paved on the bottom layer of a culture vessel, wetting the absorbent cotton by using clear water, and placing seeds with broken seed shells in the culture vessel; putting the culture vessel into an illumination incubator for culture, wherein the conditions of the incubator are as follows: the illumination time and the non-illumination time are both 12h, the temperature is set to be 20 ℃, the air humidity is set to be 70%, the illumination intensity is 1155lx light intensity, ventilation is always kept, and the moisture content of the culture medium is checked at regular time.
Example 3
a. Kneading the raisin tree fruit with dried peel, immersing the peeled seeds in water, removing the upper floating debris and seeds, and keeping the lower submerged seeds;
b. and (b) disinfecting the seeds obtained in the step (a) by using a potassium permanganate solution at normal temperature, wherein the concentration of the potassium permanganate solution is 0.4g/L, and the disinfection time is 10 min. Then cleaning the seeds with clear water, soaking the seeds in warm water at 45 ℃ for 30min, and then soaking the seeds in clear water for 1.5d at normal temperature;
c. selecting seeds with obvious imbibition, stabilizing the seeds with a pair of tweezers, keeping the seeds higher than the tweezers by 0.2cm, and using a blade to cut the seeds in a direction parallel to the ridge line to expose endosperm.
d. Using absorbent cotton with the thickness of about 0.5cm as a culture medium to be paved on the bottom layer of a culture vessel, wetting the absorbent cotton by using clear water, and placing the seeds with the broken seed shells in the culture vessel; putting the culture vessel into an illumination incubator for culture, wherein the conditions of the incubator are as follows: the illumination time and the non-illumination time are both 12h, the temperature is set to be 20 ℃, the air humidity is set to be 70%, the illumination intensity is 1155lx light intensity, ventilation is always kept, and the moisture content of the culture medium is checked at regular time.
Comparative example dry seed coat excision test
Kneading the Japanese raisin Tree fruit with dried pericarp, immersing the peeled seed in water, removing the upper floating debris and seed with tool, and retaining the lower submerged seed.
Preparing a potassium permanganate solution with the concentration of 0.4g/L, soaking the seeds for about 10min at normal temperature, cleaning the seeds with clear water, then soaking the seeds for about 30min with warm water at 45 ℃, and then airing the seeds to be dry at normal temperature.
Selecting light brown aired and dried seeds, stabilizing the seeds by using a pair of tweezers, keeping the seeds higher than the tweezers by 0.2cm, and using a blade to cut the seeds in a direction parallel to a ridge line to expose endosperm.
Using absorbent cotton with the thickness of about 0.7cm as a culture medium to be paved on the bottom layer of a culture vessel, wetting the absorbent cotton by using clear water, and placing the seeds with the broken seed shells in the culture vessel; putting the culture vessel into an illumination incubator for culture, wherein the conditions of the incubator are as follows: the illumination time and the non-illumination time are both 12h, the temperature is set to be 20 ℃, the air humidity is set to be 70%, the illumination intensity is 1155lx light intensity, ventilation is always kept, and the moisture content of the culture medium is checked at regular time.
Table 1 example 1, example 2, example 3 and comparative example hovenia dulcis thunb seed germination effects
| Means for | Seed embryo destruction rate | Time of single seed treatment | Degree of exertion | Time of germination | Germination rate |
| Example 1 | 0.5-1％ | 1-2s | Labor-saving | 3-4d | 81.1％ |
| Example 2 | 0.4-0.8％ | 1-2s | Labor-saving | 3-4d | 81.7％ |
| Example 3 | 0.3-0.9％ | 1-2s | Labor-saving | 3-4d | 82.3％ |
| Comparative example | 15-22％ | 5-10s | The application of labor | 5-7d | 75.8％ |
The method can be used for breaking the seed coats of the hovenia dulcis thunb in a time-saving and labor-saving manner, the seed embryo damage rate is extremely low, the integrity is very high, and the higher germination rate of the hovenia dulcis thunb seeds is ensured.
Claims (3)
1. A method for physically breaking the dormancy of hovenia dulcis thunb seeds is characterized by comprising the following steps:
a. kneading the raisin tree fruit with dried peel, immersing the peeled seeds in water, removing the upper floating debris and seeds, and keeping the lower submerged seeds;
b. b, disinfecting the seeds obtained in the step a by using a potassium permanganate solution at normal temperature, cleaning by using clear water, soaking the seeds for 25-35min by using warm water at 40-50 ℃, and then soaking the seeds for 1-2d by using the clear water at normal temperature;
c. selecting seeds with obvious imbibition, stabilizing the seeds with a pair of tweezers to enable the seeds to be higher than the tweezers by 0.15-0.25cm, and cutting downwards in a direction vertical to a seed ridge line or in a direction parallel to the seed ridge line by using a blade to enable endosperm to be exposed;
d. paving absorbent cotton on the bottom layer of a culture vessel, wetting the absorbent cotton with clear water, and placing seeds with broken seed shells in the culture vessel; and putting the culture vessel into a light incubator for culture.
2. The method for physically breaking the dormancy of the hovenia dulcis thunb seeds as claimed in claim 1, wherein in the step b, the concentration of the potassium permanganate solution is 0.3-0.5g/L, and the disinfection time is 10 min.
3. The method for physically breaking the dormancy of hovenia dulcis thunb seeds as claimed in claim 1, wherein the incubator conditions are as follows: the illumination time and the non-illumination time are both 12h, the temperature is set to be 20 ℃, the air humidity is set to be 70%, the illumination intensity is 1155lx light intensity, ventilation is always kept, and the moisture content of the culture medium is checked at regular time.
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| CN113892425A (en) * | 2021-11-11 | 2022-01-07 | 贵州省生物研究所 | Rapid cultivation method of hazelnut semi-aseptic seedlings |
| CN114600757B (en) * | 2022-03-25 | 2023-07-18 | 广东环境保护工程职业学院 | Seedling raising method for hovenia dulcis thunb |
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| JPS63181963A (en) * | 1987-01-22 | 1988-07-27 | Masazumi Yoshihara | Apparatus for crushing seed having rigid shell |
| CN103202127B (en) * | 2013-03-19 | 2014-12-03 | 浙江中医药大学 | Method for stopping Taxus chinensis var. mairei seed dormancy |
| CN105706561A (en) * | 2014-11-30 | 2016-06-29 | 哈尔滨弘睿翔科技开发有限公司 | Accelerating germination method for tobacco plantation |
| CN106717267B (en) * | 2016-12-12 | 2020-08-25 | 甘肃省治沙研究所 | Method for increasing germination rate of Calligonum mongolicum seeds by more than 40% |
| CN106856715A (en) * | 2017-03-01 | 2017-06-20 | 民勤县全盛永泰农业有限公司 | A kind of method for promoting cucurbita pepo seed to sprout |
| CN107690865A (en) * | 2017-07-19 | 2018-02-16 | 隆林惠侬农业科技有限公司 | A kind of germination method of sunflower seeds |
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