CN106717267B - Method for increasing germination rate of Calligonum mongolicum seeds by more than 40% - Google Patents
Method for increasing germination rate of Calligonum mongolicum seeds by more than 40% Download PDFInfo
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- CN106717267B CN106717267B CN201611138922.4A CN201611138922A CN106717267B CN 106717267 B CN106717267 B CN 106717267B CN 201611138922 A CN201611138922 A CN 201611138922A CN 106717267 B CN106717267 B CN 106717267B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for improving the germination rate of Calligonum mongolicum plant seeds to more than 40%. The method comprises the steps of immersing Calligonum mongolicum seeds in a concentrated sulfuric acid solution with a certain volume ratio for full immersion, taking out the immersed seeds, repeatedly cleaning, immersing in water, and dibbling in a culture medium fully soaked in water, wherein the culture medium is vermiculite with the particle size of 1-3 mm. The germination rate of the Calligonum mongolicum seeds treated by the method is higher than 40%, and the method has positive significance for large-area planting of Calligonum mongolicum seeds, sand control and sand fixation.
Description
Technical Field
The invention relates to a method for improving the germination rate of seeds of an psammophyte, in particular to a method for improving the germination rate of seeds of an psammophyte, namely, calligonum mongolicum.
Background
"Sha Hui Zao" (fructus Hoveniae)Calligonum mongolicum) Belonging to genus Polygonaceae (Polygonaceae) Calligonum (Polygonaceae) ((Polygonaceae))Calligonum) The plant is a drought or super-drought plant widely distributed in desert and gobi in northern arid regions (semiarid regions) in China, is also one of important vegetation for vegetation in desert in middle Asia and middle Asia, is distributed in Asia, southern Europe and northern Africa, can grow in extremely harsh arid desert regions, has the characteristics of wind erosion resistance, sand burying resistance, cutting resistance, drought resistance, barrenness resistance and the like, and is an excellent sand-fixing pioneer plant. Therefore, the Calligonum mongolicum has important research significance on the treatment and improvement of drought desertification. In addition, the tender branches can be eaten by livestock, and have higher economic value; the fruits are beautiful, are a beautiful landscape in desert, and have higher ornamental value. Seeds can be adopted for seeding and seedling raising in the breeding of the Calligonum mongolicum, but because the seed shells of the Calligonum mongolicum are hard, the germination rate of the seeds is extremely low by adopting the common soaking treatment with clear water, even the seeds hardly germinate at all, at present, methods such as seed coat damage pretreatment, 98% concentrated sulfuric acid soaking and the like are also adopted, but the germination rate does not reach 10%, and obviously how to greatly improve the germination rate of the Calligonum mongolicum seeds is an unsolved technical problem at present. Therefore, it is necessary to provide a method for improving the germination rate of the Calligonum mongolicum seeds.
Chinese patent 2014103746964 discloses a method for atriplex canescens seed germination, which comprises the following steps: soaking the atriplex canescens seeds by using concentrated sulfuric acid, cleaning the seeds after the seed soaking treatment, and placing the seeds on a vermiculite matrix for germination treatment. The technique can effectively improve the germination rate of the atriplex canescens seeds, and the data disclosed in the patent shows that the germination rate of the atriplex canescens seeds basically can reach more than 70%. Another Chinese patent 2014103784839 discloses a method for increasing germination rate of atriplex canescens seeds, which comprises soaking seeds in sodium hypochlorite solution and/or gibberellin solution, and germinating on vermiculite matrix after the seed soaking treatment. However, similar methods for seed soaking have not been satisfactory in these technical suggestions, and it is difficult to achieve satisfactory germination rates, as will be described later in the text.
Obviously, how to greatly improve the germination rate of the Calligonum mongolicum seeds is an unsolved technical problem at present.
Disclosure of Invention
The invention provides a method which can overcome the defects of the prior art and can greatly improve the germination rate of Calligonum mongolicum seeds, in particular to a method which can improve the germination rate of Calligonum mongolicum seeds to more than 40 percent.
The method for improving the germination rate of the Calligonum mongolicum seeds to be more than 40% comprises the steps of immersing the Calligonum mongolicum seeds into a concentrated sulfuric acid solution with the volume ratio of 70% -75% for full immersion, taking out the immersed seeds, kneading, cleaning, uniformly dibbling the seeds into a culture medium fully soaked by water, wherein the used culture medium is vermiculite with the particle size of 1-3 mm.
The preferable method for improving the germination rate of the Calligonum mongolicum seeds is to soak the seeds for 6-10 hours by using concentrated sulfuric acid, and tests show that the optimal time for soaking the seeds by the concentrated sulfuric acid is 6 hours.
If the seeds which are soaked in concentrated sulfuric acid, taken out and cleaned are soaked in water for 12-24 hours, the effect is better, and the seed germination time is obviously advanced by 2-3 days.
The method can greatly improve the germination rate of Calligonum mongolicum seeds, and has positive significance for large-area planting of Calligonum mongolicum and sand control and fixation.
Detailed Description
In order to search for a method for improving the germination rate of the Calligonum mongolicum seeds, the invention carries out test comparison on various technical schemes. 200 full-grain and mildew-free Calligonum rubicundunm seeds are selected as test materials, fine sand, filter paper and vermiculite culture media are fully soaked with water before the test, and all media are kept wet during germination. Dark culture at 28 ℃. In order to simulate the natural growth environment of Calligonum mongolicum, fine sand used in the test is collected from a Ministry desertification control comprehensive test station (38 degrees 34 'N and 102 degrees 58' E) in Gansu province, and the granularity of vermiculite used is 1-3 mm.
The invention is illustrated below with reference to examples.
Example 1
Soaking 200 Calligonum mongolicum seeds in 98% concentrated sulfuric acid solution for 1 min, kneading, washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 2
Soaking 200 Calligonum mongolicum seeds in 98% concentrated sulfuric acid solution for 1 min, kneading, washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with fine sand as culture medium for germination treatment, and counting the germination number and germination rate of the Calligonum mongolicum seeds.
Example 3
Soaking 200 Calligonum mongolicum seeds in 98% concentrated sulfuric acid solution for 1 min, kneading, washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish paved with filter paper for germination treatment, and counting the germination number and germination rate of the Calligonum mongolicum seeds.
Example 4
Soaking 200 Calligonum mongolicum seeds in 98% concentrated sulfuric acid solution for 10 minutes, kneading, washing with tap water for 5-10 times until the seeds are clean, then respectively dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 5
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 1 hour, kneading, washing with tap water for 5-10 times until the Calligonum mongolicum seeds are clean, uniformly dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and germination rate of the Calligonum mongolicum seeds.
Example 6
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 1 hour, kneading and washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with fine sand as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 7
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 1 hour, kneading and washing with tap water for 5-10 times until the Calligonum mongolicum seeds are clean, uniformly dropping the seeds in a culture dish paved with filter paper for germination treatment, and counting the germination number and germination rate of the Calligonum mongolicum seeds.
Example 8
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 6 hours, kneading and washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 9
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 6 hours, kneading and washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with fine sand as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 10
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 6 hours, kneading and washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish paved with filter paper for germination treatment, and counting the germination number and germination rate of the Calligonum mongolicum seeds.
Example 11
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 15 hours, kneading and washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 12
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 15 hours, kneading and washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with fine sand as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 13
Soaking 200 Calligonum mongolicum seeds in 75% concentrated sulfuric acid solution for 15 hours, kneading and washing with tap water for 5-10 times until the Calligonum mongolicum seeds are clean, uniformly dropping the seeds in a culture dish paved with filter paper for germination treatment, and counting the germination number and germination rate of the Calligonum mongolicum seeds.
Example 14
Soaking 200 Calligonum mongolicum seeds in 70% concentrated sulfuric acid solution for 6 hours, kneading and washing with tap water for 5-10 times until the seeds are clean, uniformly dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
Example 15
Soaking 200 Calligonum mongolicum seeds in 2.5% sodium hypochlorite (NaClO) solution for 10 minutes, washing with tap water for 4-5 times until the Calligonum mongolicum seeds are clean, uniformly dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and germination rate of the Calligonum mongolicum seeds.
Example 16
Soaking 200 Calligonum mongolicum seeds in 10% sodium hypochlorite (NaClO) solution for 10 minutes, washing with tap water for 4-5 times until the Calligonum mongolicum seeds are clean, uniformly dropping the seeds in a culture dish with vermiculite as a culture medium for germination treatment, and counting the germination number and the germination rate of the Calligonum mongolicum seeds.
The statistical data from examples 1-16 above are filled into the following table for comparative analysis:
the above experiments show that only under the conditions of examples 8 and 14, namely: the method comprises the steps of soaking Calligonum mongolicum seeds in 75% or 70% concentrated sulfuric acid solution for 6 hours, washing the Calligonum mongolicum seeds with water for 5-10 times until the Calligonum mongolicum seeds are clean, and then respectively dropping the Calligonum mongolicum seeds in a culture dish with vermiculite as a culture medium for germination treatment, so that the germination number and the germination rate of the Calligonum mongolicum seeds can be greatly improved. After a plurality of subsequent tests and comparative analyses, the effect is better when the seeds which are taken out and cleaned after being soaked in 70% -75% concentrated sulfuric acid for 6-10 hours are soaked in water for 12-24 hours, and the germination time of the seeds is obviously advanced by 2-3 days. And seedlings cultured by the seeds treated by 70% -75% concentrated sulfuric acid grow strongly and have no toxic or side effect on the seeds and the seedlings.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (2)
1. A method for improving the germination rate of Calligonum mongolicum seeds by more than 40% comprises the steps of placing the Calligonum mongolicum seeds in sulfuric acid solution for seed soaking, then washing the seeds with water, and then dibbling the seeds on a culture medium, wherein the Calligonum mongolicum seeds are soaked in concentrated sulfuric acid solution with the volume ratio of 70% -75% and are fully soaked, then the seeds are taken out, kneaded, washed and uniformly dibbled in the culture medium fully soaked with water, and the used culture medium is vermiculite with the particle size of 1-3 mm; the soaking time with concentrated sulfuric acid is 6 hours, and the rubbing and cleaning times are 5-10 times.
2. The method for improving the germination rate of the Calligonum rubicundunm seeds by more than 40% according to claim 1, wherein the cleaned seeds are soaked in concentrated sulfuric acid and then soaked in water for 12-24 hours.
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| CN109121552B (en) * | 2018-09-15 | 2020-11-24 | 烟台市林业科学研究所 | Method for physically breaking dormancy of hovenia dulcis thunb seeds |
| CN109845572A (en) * | 2019-04-01 | 2019-06-07 | 施阿珍 | A kind of method of more than tropic of cancer area plantation monkey-bread tree |
| CN114451096B (en) * | 2022-02-28 | 2023-03-21 | 邢台学院 | Efficient wild jujube seedling cultivation method |
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| CN101502227A (en) * | 2009-03-17 | 2009-08-12 | 中国科学院新疆生态与地理研究所 | Seedling cultivation method for Calligonum mongolicum in sand waste land |
| CN105393672B (en) * | 2016-01-21 | 2018-01-19 | 甘肃省治沙研究所 | A kind of method effectively abolished husky honey raisin tree seed dormancy, promote emergence |
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| CN103834725B (en) * | 2014-01-09 | 2016-02-17 | 中国科学院新疆生态与地理研究所 | The analytical procedure of Root of Mongolian Calligonum karyotype |
| CN104145561B (en) * | 2014-08-01 | 2015-09-23 | 兰州大学 | A kind of method for Atyiplex canescen seed sprouting |
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| CN101502227A (en) * | 2009-03-17 | 2009-08-12 | 中国科学院新疆生态与地理研究所 | Seedling cultivation method for Calligonum mongolicum in sand waste land |
| CN105393672B (en) * | 2016-01-21 | 2018-01-19 | 甘肃省治沙研究所 | A kind of method effectively abolished husky honey raisin tree seed dormancy, promote emergence |
Non-Patent Citations (1)
| Title |
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| Effects of different pre-sowing seed treatments on germination of 10 Calligonum species;Jun Ren;《Forest Ecology and Management》;20040705;第195卷(第3期);第291-300页 * |
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