CN103636313A - Germination method for schisandra sphenanthera seeds - Google Patents
Germination method for schisandra sphenanthera seeds Download PDFInfo
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- CN103636313A CN103636313A CN201310664502.XA CN201310664502A CN103636313A CN 103636313 A CN103636313 A CN 103636313A CN 201310664502 A CN201310664502 A CN 201310664502A CN 103636313 A CN103636313 A CN 103636313A
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- schisandra chinensis
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- aqueous solution
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- 230000035784 germination Effects 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000736078 Schisandra sphenanthera Species 0.000 title abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 35
- 239000002689 soil Substances 0.000 claims abstract description 18
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 15
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000003448 gibberellin Substances 0.000 claims abstract description 15
- 239000004576 sand Substances 0.000 claims abstract description 11
- 240000006079 Schisandra chinensis Species 0.000 claims description 64
- 235000008422 Schisandra chinensis Nutrition 0.000 claims description 64
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000002791 soaking Methods 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 21
- 238000002203 pretreatment Methods 0.000 claims description 17
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000005336 cracking Methods 0.000 abstract 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract 2
- 229910000366 copper(II) sulfate Inorganic materials 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 230000000052 comparative effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 230000007226 seed germination Effects 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000014284 seed dormancy process Effects 0.000 description 3
- 241000758724 Schisandraceae Species 0.000 description 2
- 230000005059 dormancy Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- SQWFNVMZGIOFHT-UHFFFAOYSA-N 2,2-bis(4-methoxyphenyl)-5,6-dimethylbenzo[h]chromene Chemical compound C1=CC(OC)=CC=C1C1(C=2C=CC(OC)=CC=2)C=CC(C(C)=C(C)C=2C3=CC=CC=2)=C3O1 SQWFNVMZGIOFHT-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001506371 Kadsura Species 0.000 description 1
- 241000233805 Phoenix Species 0.000 description 1
- 241000736075 Schisandra Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- YEFOAORQXAOVJQ-UHFFFAOYSA-N wuweizischun A Natural products C1C(C)C(C)(O)CC2=CC(OC)=C(OC)C(OC)=C2C2=C1C=C(OC)C(OC)=C2OC YEFOAORQXAOVJQ-UHFFFAOYSA-N 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a germination method for schisandra sphenanthera seeds. The schisandra sphenanthera seeds are immersed in CuSO4 solution with mass-volume concentration of 1-10mg/L or the schisandra sphenanthera seeds are immersed in gibberellin aqueous solution with mass-volume of 200-300mg/L after being immersed in the CuSO4 solution and then are buried in sand soil for germination. According to the method, the cracking rate of the schisandra sphenanthera seeds can be obviously improved, and the cracking time can be greatly shortened; therefore, the germination rate and the seedling rate of the schisandra sphenanthera seeds are effectively increased, and the seedling emergence time is shortened. A result shows that the schisandra sphenanthera seeds treated by the method disclosed by the invention can be completely cracked after being buried in the sand soil for 50 days as quickly as possible; after the seeds are buried in the sand soil for 130 days, the cracking rate of the seeds can reach 67.67-94.33 percent; after the seeds are buried in the sand soil for 140 days, the germination rate of the seeds can be 68.67-93.33%; after the seeds are buried in the sand soil for 200 days, the average seedling rate of every 100 seeds is 70-92 percent; the germination method can be widely applied to breeding and cultivating the schisandra sphenanthera seeds.
Description
Technical field
The invention belongs to field of plant variety breeding technology, be specifically related to a kind of schisandra chinensis seed dormancy of breaking, shorten the method for sprout time and raising germination rate.
Background technology
Schisandra chinensis (Schisandra sphenanthera Rehd.et Wils.) is the perennial fallen leaves bejuco of Schisandraceae (Schisandraceae) Schizandra (Schisandra Michx.), its dry mature fruit is called kadsura longepedunculata, there is convergence astringent or styptic treatment for spontaneous sweating, nourishing generate fluid, kidney tonifying is the effect such as with fixed attention, is included in < < Pharmacopoeia of People's Republic of China > > (version in 2010).Along with the further exploitation of people to schisandra chinensis medical value, market demand increases year by year, and wild schisandra chinensis, by exhaustive exploitation, causes wild resource sharply to decline, and has been put into national three-level protective natural crude drugs species.
In view of schisandra chinensis is a kind of important medicinal herbs most in use species, and medicinal exploitation be worth very large, therefore by wild foster expand resource with artificial propagation and output significant.Seminal propagation is the major way that schisandra chinensis population upgrades, but its seed dormancy phase is long, prior seed seedling-cultivating method germinates needs 7～8 months, and germination rate is low, seriously restricted the renewal of population, therefore, break seed dormancy, shorten sprout time and improve the key technology that germination rate becomes the breeding of schisandra chinensis seed seedling-raising.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of germination method of schisandra chinensis seed, and the method has been broken the dormancy of seed, and the seed germination time is short and germination rate is high.
Solving the problems of the technologies described above adopted technical scheme is comprised of following step:
1, the pretreatment of seed
The CuSO that is 1～10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 12～48 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blots the surface of the seed moisture, obtains pretreated schisandra chinensis seed; Or the CuSO that is first 1～10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 12～48 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 200～300mg/L by quality-volumetric concentration 12～48 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed.
2, the sprouting of seed
Pretreated schisandra chinensis seed is imbedded in sandy soil, and in sandy soil, sand and native volume ratio are 3:1, cover the sandy soil that 5～10cm is thick on seed, water water saturates sandy soil, controlling Germination Temperature is 8～12 ℃, every moisturizing in 3～5 days 1 time, carries out the sprouting of schisandra chinensis seed.
The optimum condition of the pre-treatment step of above-mentioned seed is: the CuSO that is 10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blots the surface of the seed moisture, obtains pretreated schisandra chinensis seed.
The pre-treatment step 1 of above-mentioned seed is more preferably: the CuSO that is first 10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 24 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 200～300mg/L by quality-volumetric concentration 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed.
The optimum condition of the pre-treatment step 1 of above-mentioned seed is: the CuSO that is first 10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 24 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 250mg/L by quality-volumetric concentration 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed.
The present invention directly uses CuSO
4the aqueous solution is sprouted processing to schisandra chinensis seed, or uses CuSO
4the aqueous solution is sprouted processing with the gibberellin aqueous solution after processing again, significantly improved schisandra chinensis seed breach rate, greatly shortened the breach time of seed, obvious for the effect of schisandra chinensis seed breaking dormancy, and schisandra chinensis percentage of seedgermination and planting percent have effectively been improved, when improving seed planting percent, than the seedling-growing time of traditional seedling-cultivating method, 30 days have been shortened.Result of the test shows, adopt the schisandra chinensis seed after the inventive method is processed can hide and within 50 days, reach breach peak at sand the soonest, husky Tibetan after 130 days, the breach rate of seed can reach 67.67%～94.33%, husky Tibetan 140 days, the germination rate of seed can reach 68.67%～93.33%, husky Tibetan 200 days, average number of seedling 70～92 strains of 100 seeds, are suitable for extensive use in schisandra chinensis breeding cultivation.
Embodiment
Below in conjunction with embodiment, the present invention is described in more detail, but the invention is not restricted to these embodiment.
Embodiment of the present invention schisandra chinensis seed used is from picking up from the wild schisandra chinensis ripening fruits of fostering base of phoenix town, Zuoshui County, Shaanxi Province schisandra chinensis in September, 2011: get in same population wild schisandra chinensis berry red fruit thoroughly, clear water soaks, rub removal pulp, through rinsing, floating immature seed or the empty grain of removing of water, choose and be sunken to water-bed portion seed, room temperature is air-dry.
Embodiment 1
1, the pretreatment of seed
Get 100 schisandra chinensis seeds, the CuSO that is 10mg/L by quality-volumetric concentration
4aqueous solution soaking 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blots the surface of the seed moisture, obtains pretreated schisandra chinensis seed.
2, the sprouting of seed
Pretreated schisandra chinensis seed is imbedded in sandy soil, in sandy soil, sand and native volume ratio are 3:1, on seed, cover the sandy soil that 8cm is thick, water water saturates sandy soil, controlling Germination Temperature is 10 ℃, every moisturizing in 3 days 1 time, make sandy soil keep moistening shape, without ponding, guarantee the water demand in during Seed Germination, carry out the sprouting of schisandra chinensis seed.
Embodiment 2
In the pre-treatment step 1 of the seed of embodiment 1, the CuSO that quality-volumetric concentration used is 10mg/L
4the CuSO that the aqueous solution is 1mg/L by quality-volumetric concentration
4the aqueous solution is replaced, and other steps are identical with embodiment 1.
Embodiment 3
In the pre-treatment step 1 of the seed of embodiment 1, the CuSO that quality-volumetric concentration used is 10mg/L
4the CuSO that the aqueous solution is 5mg/L by quality-volumetric concentration
4the aqueous solution is replaced, and other steps are identical with embodiment 1.
Embodiment 4
In the pre-treatment step 1 of the present embodiment seed, get 100 schisandra chinensis seeds, the CuSO that is 10mg/L by quality-volumetric concentration
4aqueous solution soaking 24 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 250mg/L by quality-volumetric concentration 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed.Other steps are identical with embodiment 1.
Embodiment 5
In the pre-treatment step 1 of the seed of embodiment 4, the gibberellin aqueous solution that the gibberellin aqueous solution that quality-volumetric concentration used is 250mg/L is 200mg/L by quality-volumetric concentration is replaced, and other steps are identical with embodiment 4.
Embodiment 6
In the pre-treatment step 1 of the seed of embodiment 4, the gibberellin aqueous solution that the gibberellin aqueous solution that quality-volumetric concentration used is 250mg/L is 300mg/L by quality-volumetric concentration is replaced, and other steps are identical with embodiment 4.
Comparative example 1
In the pre-treatment step 1 of the seed of embodiment 1, get 100 schisandra chinensis seeds, be soaked in water 24 hours, other steps are identical with embodiment 1.
Comparative example 2
In the pre-treatment step 1 of the seed of embodiment 1, get 100 schisandra chinensis seeds, the CuSO that is 20mg/L by quality-volumetric concentration
4aqueous solution soaking 24 hours, other steps are identical with embodiment 1.
Comparative example 3
In the pre-treatment step 1 of the seed of embodiment 1, get 100 schisandra chinensis seeds, the gibberellin aqueous solution soaking that is 250mg/L by quality-volumetric concentration 24 hours, other steps are identical with embodiment 1.
Embodiment 7
In the pre-treatment step 1 of the seed of embodiment 4, get 100 schisandra chinensis seeds, the CuSO that is 1mg/L by quality-volumetric concentration
4aqueous solution soaking 24 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 250mg/L by quality-volumetric concentration 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed.Other steps are identical with embodiment 1.
Embodiment 8
In the pre-treatment step 1 of the seed of embodiment 1～7, the soak time of schisandra chinensis seed all extends to 48 hours, and other steps are identical with corresponding embodiment.
Embodiment 9
In the pre-treatment step 1 of the seed of embodiment 1～7, the soak time of schisandra chinensis seed all foreshortens to 12 hours, and other steps are identical with corresponding embodiment.
In order to prove beneficial effect of the present invention, inventor has carried out observing statistics to the sprouting situation of schisandra chinensis seed in embodiment 1～6 and comparative example 1～3, and husky Tibetan starts to add up breach number for 30 days, calculates breach rate, every 20 days statistics once, to 130 days only; After seed breach, the routine observation seed situation of sprouting, calculates germination rate, occurs after seedling, every 10 days statistics once, calculates seeding rate.Result of the test is the mean value of 3 parallel tests in Table result in 1(table).
The impact of table 1 different disposal method on schisandra chinensis seed germination
From the result of the test in table 1, schisandra chinensis seed after processing in embodiment 1～3 is all hidden and within 110 days, is reached breach peak at sand, peak breach number reaches 43～46.33, husky Tibetan after 130 days, the breach rate of seed can reach 67.67%～81.33%, husky Tibetan 140 days, the germination rate of seed can reach 68.67%～77%, husky Tibetan 200 days, the average number of seedling of 100 seeds reaches 69.67～76 strains, schisandra chinensis seed after processing in embodiment 4～6 is all hidden and within 50 days, is reached breach peak at sand, peak breach number reaches 55～61, husky Tibetan after 130 days, the breach rate of seed can reach 83.33%～94.33%, husky Tibetan 140 days, the germination rate of seed can reach 88%～93.33%, husky Tibetan 200 days, the average number of seedling of 100 seeds reaches 88.67～92 strains, breach rate, germination rate, number of seedling is all apparently higher than comparative example 1～3, illustrate that the inventive method has stronger short sprouting effect and sprout regularity high.
Claims (4)
1. a germination method for schisandra chinensis seed, is characterized in that it is comprised of following step:
(1) pretreatment of seed
The CuSO that is 1～10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 12～48 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blots the surface of the seed moisture, obtains pretreated schisandra chinensis seed;
Or the CuSO that is first 1～10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 12～48 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 200～300mg/L by quality-volumetric concentration 12～48 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed;
(2) sprouting of seed
Pretreated schisandra chinensis seed is imbedded in sandy soil, and in sandy soil, sand and native volume ratio are 3:1, cover the sandy soil that 5～10cm is thick on seed, water water saturates sandy soil, controlling Germination Temperature is 8～12 ℃, every moisturizing in 3～5 days 1 time, carries out the sprouting of schisandra chinensis seed.
2. the germination method of schisandra chinensis seed according to claim 1, is characterized in that: in the pre-treatment step of described seed (1), and the CuSO that is 10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blots the surface of the seed moisture, obtains pretreated schisandra chinensis seed.
3. the germination method of schisandra chinensis seed according to claim 1, is characterized in that: in the pre-treatment step of described seed (1), and the CuSO that is first 10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 24 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 200～300mg/L by quality-volumetric concentration 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed.
4. the germination method of schisandra chinensis seed according to claim 3, is characterized in that: in the pre-treatment step of described seed (1), and the CuSO that is first 10mg/L by quality-volumetric concentration by schisandra chinensis seed
4aqueous solution soaking 24 hours, take out, with clear water, rinse the Liquid Residue of the surface of the seed, blot the surface of the seed moisture, then the gibberellin aqueous solution soaking that is 250mg/L by quality-volumetric concentration 24 hours, takes out, and rinses the Liquid Residue of the surface of the seed with clear water, blot the surface of the seed moisture, obtain pretreated schisandra chinensis seed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310664502.XA CN103636313B (en) | 2013-12-09 | 2013-12-09 | The germination method of schisandra chinensis seed |
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| CN201310664502.XA CN103636313B (en) | 2013-12-09 | 2013-12-09 | The germination method of schisandra chinensis seed |
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| CN103636313A true CN103636313A (en) | 2014-03-19 |
| CN103636313B CN103636313B (en) | 2015-10-28 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766136A (en) * | 2016-03-10 | 2016-07-20 | 中国农业科学院特产研究所 | Method for increasing germination rate of seed of schisandra chinensis |
| CN106068764A (en) * | 2016-06-24 | 2016-11-09 | 叶祥蕤 | The quick germination method of Fructus Schisandrae Sphenantherae seed |
| CN113943190A (en) * | 2021-12-09 | 2022-01-18 | 陕西师范大学 | Radix scrophulariae bud water-retention root-promotion probiotic powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0856423A (en) * | 1994-08-25 | 1996-03-05 | Sumitomo Chem Co Ltd | Method for improving germination of solanaceous seed |
| CN102986331A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Treatment method of seeds of fructus schisandrae |
-
2013
- 2013-12-09 CN CN201310664502.XA patent/CN103636313B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0856423A (en) * | 1994-08-25 | 1996-03-05 | Sumitomo Chem Co Ltd | Method for improving germination of solanaceous seed |
| CN102986331A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Treatment method of seeds of fructus schisandrae |
Non-Patent Citations (3)
| Title |
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| 李大勇 等: "五味子育苗技术要点", 《内蒙古林业调查设计》 * |
| 王祥明: "南五味子GAP规范化栽培技术", 《陕西林业科技》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766136A (en) * | 2016-03-10 | 2016-07-20 | 中国农业科学院特产研究所 | Method for increasing germination rate of seed of schisandra chinensis |
| CN105766136B (en) * | 2016-03-10 | 2018-12-11 | 中国农业科学院特产研究所 | A method of improving Schisandra chinensis percentage of seedgermination |
| CN106068764A (en) * | 2016-06-24 | 2016-11-09 | 叶祥蕤 | The quick germination method of Fructus Schisandrae Sphenantherae seed |
| CN113943190A (en) * | 2021-12-09 | 2022-01-18 | 陕西师范大学 | Radix scrophulariae bud water-retention root-promotion probiotic powder |
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