CN105766136A - Method for increasing germination rate of seed of schisandra chinensis - Google Patents

Method for increasing germination rate of seed of schisandra chinensis Download PDF

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Publication number
CN105766136A
CN105766136A CN201610136311.XA CN201610136311A CN105766136A CN 105766136 A CN105766136 A CN 105766136A CN 201610136311 A CN201610136311 A CN 201610136311A CN 105766136 A CN105766136 A CN 105766136A
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China
Prior art keywords
seed
river sand
fructus schisandrae
schisandrae chinensis
germination percentage
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CN201610136311.XA
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CN105766136B (en
Inventor
许培磊
艾军
杨义明
秦红艳
张宝香
王振兴
范书田
李晓艳
刘迎雪
孙丹
王春伟
赵滢
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Institute Special Animal and Plant Sciences CAAS
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Institute Special Animal and Plant Sciences CAAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like

Abstract

The invention relates to the field of crop cultivation, in particular to a method for increasing the germination rate of seeds of schisandra chinensis. The method comprises the following steps: pretreating the seeds, namely, sequentially soaking the seeds of schisandra chinensis in a salt solution containing Cu<2+> and a gibberellins solution; enabling the seeds to germinate, namely, combining the pretreated seeds with wet sand, bagging, covering with wet sand, placing in an environment of 15-20 DEG C for 15-20 days and an environment of 0-2 DEG C for 2-3 months in sequence, and further moving to an environment of 19-21 DEG C to allow standing still for 13-17 days. According to the method, graded seeds are adopted for coprocessing of Cu<2+> and hormone pretreatment and variable temperature sand-storage, the purpose of increasing the germination rate of the seeds is achieved, and the seed germination uniformity is improved, so that later seedling cultivation can be easily managed, and the labor can be saved.

Description

A kind of method improving seed of Fructus Schisandrae Chinensis germination percentage
Technical field
The present invention relates to field of crop cultivation, in particular to a kind of method improving seed of Fructus Schisandrae Chinensis germination percentage.
Background technology
Fructus Schisandrae Chinensis is Magnoliacea plant, perennial fallen leaves woody climber, is that a kind of main product is in the large genuine Chinese crude drug in China northeast.What Fructus Schisandrae Chinensis wild resource destroyed in recent years is more serious, and its market demand is growing so that the artificial culture of Fructus Schisandrae Chinensis quickly grows.
Fructus Schisandrae Chinensis Seedling propagation is to provide the Main Means of graft stock, is also the current Fructus Schisandrae Chinensis major measure of building garden.But its seed dormancy phase length, germination percentage are relatively low, and germinating time is inconsistent, thus cause that seed of Fructus Schisandrae Chinensis nursery emergence rate is low, the problem such as uneven of emerging.It is reported, seed of Fructus Schisandrae Chinensis rest period was up to 5~6 months, and germination percentage is only 1.4%.Therefore, how research improves seed of Fructus Schisandrae Chinensis germination percentage, shortens its dormancy time, obtains the neat breaking dormancy seed of breach is the element task improving schisandra cultivating efficiency, and the utilization of Fructus Schisandrae Chinensis seedling and the development of cultivation industry is significant.
At present, several methods breaking Fructus Schisandrae Chinensis dormancy reported relate generally to temperature and hormone induction processes, and two kinds of methods are respectively arranged with quality.Wherein high/low temperature intermittent warming germination percentage can reach 84.5%, but the time of process needs 6 months;The hormone treatment method cycle is relatively short, but the germination percentage of seed can only achieve 64%, not satisfactory.
In view of this, the special proposition present invention.
Summary of the invention
It is an object of the invention to provide a kind of method improving seed of Fructus Schisandrae Chinensis germination percentage, described method can effectively solve the problem that prior art seed of Fructus Schisandrae Chinensis germination percentage is low, dormancy time length, germination regularity are poor.
In order to realize the above-mentioned purpose of the present invention, spy by the following technical solutions:
A kind of method improving seed of Fructus Schisandrae Chinensis germination percentage, comprises the following steps:
1), Seeds preprocess: seed of Fructus Schisandrae Chinensis is successively soaked in containing Cu2+Saline solution and Gibberellins solution in;
2), the sprouting of seed: pretreated seed is mixed homogeneously with wet river sand and packs, cover with wet river sand again, and successively place 15~20 days, 0~2 DEG C environment placement 2~3 months in 15~20 DEG C of environment, then go to placement 13~17 days in the environment of 19~21 DEG C.
Described wet river sand humidity controls constant all the time.
The application is technically characterized in that, by Cu2+Process, HORMONE TREATMENT combines with intermittent warming, thus effectively promoting kind of an embryo after-ripening, shortening the seed dormancy time, improving percentage of seedgermination and regularity, and being easily managed, saves labour force.
Seed germination is the initial period of most of flowering plant growth promoter, and the hormonal readiness by seed itself regulates and controls, and simultaneously also by environmental effects such as exterior light photograph, temperature, is a complicated process of external environment condition and seed own metabolism reciprocal effect.
CuSO4Process seed and can soften seed coat, and play disinfective action.
Gibberellins (Gibberellin, GA), also referred to as Ji Beisu, is a Plant Hormone, growth regulation and affect various growth courses, including promote stem elongation, germinate, bloom, the aging suppression of Sex, leaf and fruit.
Intermittent warming is conducive to changing the retractility of seed coat and causing seed coat to break, and stimulates seed metabolism, thus also has the seed dormant effect of releasing.
Three of the above physicochemical irritation is carried out rational matched combined by the application, has the effect brought out one's strengths to make up for one's weaknesses, and namely maintains very high seed germination rate, is effectively shortened again the seed dormancy time.
Preferably, the method improving seed of Fructus Schisandrae Chinensis germination percentage as above, in step 1) in:
Described containing Cu2+The CuSO that saline solution is 8~12g/L4Solution;
Described Gibberellins solution is the GA of 1.6~2.1g/L3Aqueous solution.
GA3No. CAS be 77-06-5, molecular formula is C19H22O6。GA3The effect broken seed dormancy, promote seed germination can be played.
It is further preferred that in the method improving seed of Fructus Schisandrae Chinensis germination percentage as above:
Seed of Fructus Schisandrae Chinensis is at described CuSO4The time soaked in solution is 8~12 minutes, at GA3The time soaked in aqueous solution is 22~26 hours.
Preferably, the method improving seed of Fructus Schisandrae Chinensis germination percentage as above, the particle diameter of described seed of Fructus Schisandrae Chinensis is 3.5~4.5mm.
There is larger difference in the seed of Fructus Schisandrae Chinensis quality of different regions, bigger seed is with relatively low compared with seedlet germination percentage, and proportion is different, and seed, after sieve classification, can first reject more abnormal seed.
Preferably, the method improving seed of Fructus Schisandrae Chinensis germination percentage as above, in step 2) in:
When pretreated seed mixes with wet river sand, it is 1:3~1:4 according to the ratio of volume parts ratio, described seed and described wet river sand.
It is further preferred that the method improving seed of Fructus Schisandrae Chinensis germination percentage as above, described wet river sand is rinsed well and before use through 120~125 DEG C of sterilizings 15~25 minutes.
Preferably, the method improving seed of Fructus Schisandrae Chinensis germination percentage as above:
In step 1) in: also include before changing immersion bubble being rinsed well by seed with clear water and drying;
In step 2) in: also include before being mixed with wet river sand by pretreated seed being rinsed well by seed and drying.
Seed rinsed well and dries the solution effects overlong time that can prevent used by previous step, being also beneficial to prevent the solution used by previous step from subsequent solution/material being polluted.
Preferably, the as above method improving seed of Fructus Schisandrae Chinensis germination percentage, in step 2) in, described pretreated seed mixed homogeneously with wet river sand and pack, then specifically including by the step of river sand covering of wetting:
Load in the seed packet of water-permeable and air permeable after pretreated seed is mixed homogeneously with wet river sand, described seed packet is placed in the container filling wet river sand, and use container described in the plastic bag enclosure after the wet river sand of filling around described seed packet;
And, container places 15~20 days, 0~2 DEG C environment placement 2~3 months successively in 15~20 DEG C of environment, then goes in the process placing 13~17 days in the environment of 19~21 DEG C:
By described seed packet every upset in 5~9 days once.
It is further preferred that the thickness of the wet river sand filled around described seed packet is 3~5cm.
Upset can ensure that the seed moisture in seed packet is uniform.
Preferably, the method improving seed of Fructus Schisandrae Chinensis germination percentage as above, the water content of described wet river sand is the 45~55% of husky maximum water-holding capacity.
During concrete operations, the water-holding capacity of wet river sand with after taking out with hold state that is agglomerating and that do not drip and be as the criterion.
Compared with prior art, the invention have the benefit that
1), the present invention adopt classification after seed carry out Cu2+And Hormones pretreatment and alternating temperature Sand culture, reach to improve the purpose of percentage of seedgermination;
2), the method improving seed of Fructus Schisandrae Chinensis germination percentage that provides of the application easily operated, and Application way also can improve the regularity of germination, so that the cultivation of follow-up seedling is easily managed, saves labour force.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below.
Fig. 1 is the effectiveness comparison result of alternating temperature Sand culture and low temperature Sand culture in experimental example;
Fig. 2 be in experimental example by screen for seeds classification that seed diameter is 3.5mm and 4.5mm time picture;
Fig. 3 is through hormon and/or Cu in experimental example2+The impact on seed of Fructus Schisandrae Chinensis germination percentage after intermittent warming is carried out after process.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, it will be appreciated by those skilled in the art that the following example is merely to illustrate the present invention, and are not construed as restriction the scope of the present invention.Unreceipted actual conditions person in embodiment, conventionally the condition of condition or manufacturer's suggestion carries out.Agents useful for same or the unreceipted production firm person of instrument, be and can pass through the commercially available conventional products bought and obtain.
Embodiment 1
A kind of method improving seed of Fructus Schisandrae Chinensis germination percentage, comprises the following steps:
S11, Seeds preprocess: seed of Fructus Schisandrae Chinensis is successively soaked in containing Cu2+Saline solution and Gibberellins solution in;
S12, seed sprouting: pretreated seed is mixed homogeneously with wet river sand and packs, cover with wet river sand again, and successively place 15~20 days, 0~2 DEG C environment placement 2~3 months in 15~20 DEG C of environment, then go to placement 13~17 days in the environment of 19~21 DEG C.
Embodiment 2
A kind of method improving seed of Fructus Schisandrae Chinensis germination percentage, comprises the following steps:
S21, seed grading
By the screen for seeds classification that clean seed diameter is 3.5mm and 4.5mm, it is chosen at the seed within the scope of this, is placed in seed packet;
S22, Seeds preprocess
By seed packet in the CuSO of 12g/L4Solution soaks 8 minutes, rinse and blot the solution of the surface of the seed with water after, forwards the GA of 1.6g/L to3Solution continues soak 26 hours;
S23, river sand pretreatment
River sand is clean with running water, it is placed in high-pressure sterilizing pot 120 DEG C of sterilizings 25 minutes, dries in the air cool after taking-up and regulate humidity;
S24, seed sand are hidden
Seed use water after immersion rinses, and adds wet river sand with the ratio of wet river sand 1:4 according to seed, mix homogeneously with wet seed in seed packet, put in the flowerpot being covered with wet river sand, filling the wet sand of 3cm around seed packet, plastic bag on flowerpot outer mask, to prevent moisture loss;
S25, intermittent warming
Flowerpot is positioned in the environment of 20 DEG C and places 15 days, then switch in the environment of 2 DEG C and place 3 months, places 17 days in sowing forward to 19 DEG C of temperature environments;Described wet river sand humidity controls constant all the time;
S26, daily management
Every 9 days, seed packet positive and negative is overturn once, observe river sand humidity, timely moisturizing;
In above procedure, river sand humidity remains at 45%.
Embodiment 3
A kind of method improving seed of Fructus Schisandrae Chinensis germination percentage, comprises the following steps:
S31, seed grading
By the screen for seeds classification that clean seed diameter is 3.5mm and 4.5mm, it is chosen at the seed within the scope of this, is placed in seed packet;
S32, Seeds preprocess
By seed packet in the CuSO of 8g/L4Solution soaks 12 minutes, rinse and blot the solution of the surface of the seed with water after, forwards the GA of 2.1g/L to3Solution continues soak 22 hours;
S33, river sand pretreatment
River sand is clean with running water, it is placed in high-pressure sterilizing pot 125 DEG C of sterilizings 15 minutes, dries in the air cool after taking-up and regulate humidity;
S34, seed sand are hidden
Seed use water after immersion rinses, and adds wet river sand with the ratio of wet river sand 1:3 according to seed, mix homogeneously with wet seed in seed packet, put in the flowerpot being covered with wet river sand, filling the wet sand of 5cm around seed packet, plastic bag on flowerpot outer mask, to prevent moisture loss;
S35, intermittent warming
Flowerpot is positioned in the environment of 15 DEG C and places 20 days, then switch in the environment of 0 DEG C and place 2 months, places 16 days in sowing forward to 21 DEG C of temperature environments;Described wet river sand humidity controls constant all the time;
S36, daily management
Every 5 days, seed packet positive and negative is overturn once, observe river sand humidity, timely moisturizing;
In above procedure, river sand humidity remains at 55%.
Embodiment 4
A kind of method improving seed of Fructus Schisandrae Chinensis germination percentage, comprises the following steps:
S41, seed grading
By the screen for seeds classification that clean seed diameter is 3.5mm and 4.5mm, it is chosen at the seed within the scope of this, is placed in seed packet;
S42, Seeds preprocess
By seed packet in the CuSO of 10g/L4Solution soaks 10 minutes, rinse and blot the solution of the surface of the seed with water after, forwards the GA of 1.88g/L to3Solution continues soak 24 hours;
S43, river sand pretreatment
River sand is clean with running water, it is placed in high-pressure sterilizing pot 121 DEG C of sterilizings 20 minutes, dries in the air cool after taking-up and regulate humidity;
S44, seed sand are hidden
Seed use water after immersion rinses, and adds wet river sand with the ratio of wet river sand 1:3 according to seed, mix homogeneously with wet seed in seed packet, put in the flowerpot being covered with wet river sand, filling the wet sand of 4cm around seed packet, plastic bag on flowerpot outer mask, to prevent moisture loss;
S45, intermittent warming
Flowerpot is positioned in the environment of 18 DEG C and places 17 days, then switches into 2 first quarter moons of placement in the environment of 1 DEG C, places 15 days in sowing forward to 20 DEG C of temperature environments;Described wet river sand humidity controls constant all the time;
S46, daily management
Every 7 days, seed packet positive and negative is overturn once, observe river sand humidity, timely moisturizing;
In above procedure, river sand humidity remains at 50%.
Experimental example
The effectiveness comparison of alternating temperature Sand culture and low temperature Sand culture:
1, seed resource is selected
Take the six parts of resources gathering numbering respectively " 2013-5 ", " 2013-7 ", " 2013-8 ", " 2013-10 ", " 2013-38 " and " 2013-41 " for 2013, dry standby after washing kind.
2, seed is soaked 2~3 days, change water every day once, seed and sterilizing wet husky mix homogeneously according to 1:3 ratio be divided into two parts, it is individually positioned in flowerpot, a for intermittent warming: flowerpot is positioned in 15~20 DEG C of environment 15~20 days, go to 2~March in 0~2 DEG C of environment, within first 15 days, go in 20 DEG C of temperature environments in sowing and place 15 days;Another part is used for K cryogenic treatment: March in 0~2 DEG C of environment, within first 15 days, goes in 20 DEG C of temperature environments in sowing and places 15 days.
Experimental result is as shown in Figure 1.From experimental result it can be seen that alternating temperature Sand culture different resource seed, average germination percentage is 76.74%, and the average germination percentage of low temperature Sand culture is 33.92%;Relative to K cryogenic treatment, intermittent warming can make germination percentage improve 1.26 times.
In addition, can be seen that from the above, the percentage of seedgermination of different germ plasm resources differs greatly, the minimum germination percentage that Conventional cryogenic processes is only 3.04%, reach as high as 65.44%, the present invention, in order to the difference of germ plasm resource be minimized, makes processing method be common to each germplasm resources of Schisandra chinensis, and first different resource seed has carried out mixing and staged care;Secondly, in order to improve the germination percentage of intermittent warming once again, the seed hormon before intermittent warming has been processed by the present invention, and germination percentage is compared.
Experiment after being adjusted is as follows:
Hormon and/or the metal ion impact on germination percentage before alternating temperature Sand culture
1, seed grading
By the screen for seeds classification that clean seed diameter is 3.5mm and 4.5mm, selected seed width seed (Fig. 2 is screen for seeds picture used by screening process) within the scope of this, it is placed in seed packet;
There is larger difference in the seed of Fructus Schisandrae Chinensis quality of different regions, bigger seed is with relatively low compared with seedlet germination percentage, and proportion is different, and seed, after sieve classification, can first reject more abnormal seed.
2, the pretreatment of seed and Sha Zang
The processing mode of seed is consistent with embodiment 4, is different in that:
Seed clean after classification is divided into 3 parts, processes seed with three kinds of hormone prescriptions respectively, A:TDZ0.10g/L24 hour;B:6-BA0.05g/L+GA30.15g/L24 hour;C:10g/LCuSO410 minutes+1.88g/LGA324 hours (embodiment 4);.
Intermittent warming and daily management are with embodiment 4.
Experimental result is as it is shown on figure 3, when alternating temperature sand Tibetan, the germination percentage of seed is affected difference by hormon, with the formula 10g/LCuSO of C formula and embodiment 4410 minutes+1.88g/LGA3Within 24 hours, effect is best, and germination percentage can be made to reach 92.86%.
From experimental result it can be seen that the present invention carries out Cu by the seed after adopting classification2+And Hormones pretreatment and alternating temperature sand hides collaborative process, reach to improve the purpose of percentage of seedgermination, and improve the regularity of germination, so that the cultivation of follow-up seedling is easily managed.
Although illustrate and describing the present invention with specific embodiment, however it will be appreciated that may be made that when without departing substantially from the spirit and scope of the present invention many other change and amendment.It is, therefore, intended that include all such changes and modifications belonging in the scope of the invention in the following claims.

Claims (10)

1. the method improving seed of Fructus Schisandrae Chinensis germination percentage, it is characterised in that comprise the following steps:
1), Seeds preprocess: seed of Fructus Schisandrae Chinensis is successively soaked in containing Cu2+Saline solution and Gibberellins solution in;
2), the sprouting of seed: pretreated seed is mixed homogeneously with wet river sand and packs, cover with wet river sand again, and successively place 15~20 days, 0~2 DEG C environment placement 2~3 months in 15~20 DEG C of environment, then go to placement 13~17 days in the environment of 19~21 DEG C.
2. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 1, it is characterised in that in step 1) in:
Described containing Cu2+The CuSO that saline solution is 8~12g/L4Solution;
Described Gibberellins solution is the GA of 1.6~2.1g/L3Aqueous solution.
3. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 2, it is characterised in that:
Seed of Fructus Schisandrae Chinensis is at described CuSO4The time soaked in solution is 8~12 minutes, at GA3The time soaked in aqueous solution is 22~26 hours.
4. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 1, it is characterised in that the particle diameter of described seed of Fructus Schisandrae Chinensis is 3.5~4.5mm.
5. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 1, it is characterised in that in step 2) in:
When pretreated seed mixes with wet river sand, it is 1:3~1:4 according to the ratio of volume parts ratio, described seed and described wet river sand.
6. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 5, it is characterised in that described wet river sand is rinsed well and before use through 120~125 DEG C of sterilizings 15~25 minutes.
7. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 1, it is characterised in that:
In step 1) in: also include before changing immersion bubble being rinsed well by seed with clear water and drying;
In step 2) in: also include before being mixed with wet river sand by pretreated seed being rinsed well by seed and drying.
8. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 1, it is characterised in that in step 2) in, described pretreated seed mixed homogeneously with wet river sand and packs, then the step covered with wet river sand specifically includes:
Load in the seed packet of water-permeable and air permeable after pretreated seed is mixed homogeneously with wet river sand, described seed packet is placed in the container filling wet river sand, and use container described in the plastic bag enclosure after the wet river sand of filling around described seed packet;
And, container places 15~20 days, 0~2 DEG C environment placement 2~3 months successively in 15~20 DEG C of environment, then goes in the process placing 13~17 days in the environment of 19~21 DEG C:
By described seed packet every upset in 5~9 days once.
9. the method for raising seed of Fructus Schisandrae Chinensis germination percentage according to claim 8, it is characterised in that the thickness of the wet river sand filled around described seed packet is 3~5cm.
10. the method improving seed of Fructus Schisandrae Chinensis germination percentage according to any one of claim 1~9, it is characterised in that the water content of described wet river sand is the 45~55% of husky maximum water-holding capacity.
CN201610136311.XA 2016-03-10 2016-03-10 A method of improving Schisandra chinensis percentage of seedgermination Active CN105766136B (en)

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CN108718598A (en) * 2018-05-09 2018-11-02 吉林农业科技学院 A method of improving codonopsis lanceolata percentage of seedgermination
CN112314625A (en) * 2020-11-12 2021-02-05 东莞市东阳光冬虫夏草研发有限公司 Pre-seeding pretreatment agent for bulbil and application thereof
CN114128707A (en) * 2021-11-26 2022-03-04 沈阳药科大学 Schisandra chinensis germplasm resource preservation method

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108718598A (en) * 2018-05-09 2018-11-02 吉林农业科技学院 A method of improving codonopsis lanceolata percentage of seedgermination
CN108718598B (en) * 2018-05-09 2021-01-08 梧州学院 Method for improving germination rate of Codonopsis lanceolata seeds
CN112314625A (en) * 2020-11-12 2021-02-05 东莞市东阳光冬虫夏草研发有限公司 Pre-seeding pretreatment agent for bulbil and application thereof
CN114128707A (en) * 2021-11-26 2022-03-04 沈阳药科大学 Schisandra chinensis germplasm resource preservation method

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