CN112314625A - Pre-seeding pretreatment agent for bulbil and application thereof - Google Patents
Pre-seeding pretreatment agent for bulbil and application thereof Download PDFInfo
- Publication number
- CN112314625A CN112314625A CN202011261266.3A CN202011261266A CN112314625A CN 112314625 A CN112314625 A CN 112314625A CN 202011261266 A CN202011261266 A CN 202011261266A CN 112314625 A CN112314625 A CN 112314625A
- Authority
- CN
- China
- Prior art keywords
- solution
- treatment
- bulbil
- buds
- kinetin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Toxicology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of traditional Chinese medicine planting, and particularly relates to a pretreatment agent before pearl bud seeding and application thereof. The pre-seed pretreatment agent comprises a first treatment liquid and a second treatment liquid, wherein the first treatment liquid is a copper sulfate aqueous solution, and the second treatment liquid is a plant growth regulator solution. In addition, the invention discloses the application of the pre-seed pretreatment agent in the pre-seeding pretreatment of the polygonum bulbil and a method for the pre-seeding pretreatment of the polygonum bulbil. The pretreatment agent before the pearl buds are planted can relieve the dormancy of the pearl buds in special forms in alpine regions, so that the polygonum bulbiferum is planted in the whole season suitable for growth, the rotten rate of the pearl buds can be obviously reduced, and the germination vigor and the germination rate of the pearl buds can be obviously improved.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine planting, and particularly relates to a pretreatment agent before pearl bud seeding and application thereof.
Background
Polygonum bulbiferum L, also known as Scorpion seven, sorghum halepense and dyed cloth, is a perennial herb of Polygonum of Polygonaceae. The ecological floating fertilizer is widely distributed in Asia, North America and Europe, and mainly grows on grasslands, beaches and bushes with the elevation of 1200-5100 m, such as Sichuan, Tibet, Qinghai, Gansu, Yunnan, Jilin, inner Mongolia and Hubei, in China. Polygonum bulboruifolium is a widely used Chinese herbal medicine, and has the functions of reducing fever, stopping diarrhea, regulating menstruation, astringing and stopping bleeding by using rhizome as a medicine; the rhizome and fruit are rich in starch, and can be used for brewing wine, and the young stem and leaf can be used as feed. The whole grass is mashed and made into powder or solution which can prevent and control crop pests. Except being used as the traditional Chinese medicine, the polygonum bulbiferum has the rhizome which is also the main feed for the host larvae of the cordyceps sinensis which is a rare medicinal material in China, so the polygonum bulbiferum has higher economic value.
In recent years, the utilization of the rhizome of the polygonum bulbiferum mainly depends on wild digging, the research on artificial cultivation and breeding technology is less, and the shortage and even extinction of wild resources can be caused after the research, so that the market demand can not be met. At present, the polygonum bulbiferum begins to be artificially planted.
The spike axis of the polygonum bulbiferum can grow the bulbils for asexual propagation, and can differentiate the buds to blossom and fertilize, and the sexual process is completed to produce seeds. The bulbil and the seed are two kinds of reproductive organs with completely different properties and structures, and can be formed on the spica axis by differentiation sequentially or alternatively. However, the capacity of vegetative propagation of the polygonum bulbiferum generated by differentiation on the inflorescence axis of the polygonum bulbiferum which grows in the Qinghai-Tibet plateau under the ecological conditions of common high cold, short growing season and low temperature is obviously higher than the sexual propagation capacity of the flowers and fruits, and the vegetative propagation of a large amount of the polygonum bulbiferum generated in the early growth stage is a main adaptive propagation mode of the polygonum bulbiferum in the high cold area; and the polygonum bulbosum growing in the Qinghai-Tibet plateau area has special bulbil shape, dark brown appearance, cone-shaped structure, hard texture and deep and long dormancy period, and the germination rate is extremely low under proper germination conditions after harvesting. Under the prior art, the harvested bulbils in the plateau area can not normally germinate under the suitable environmental conditions after being directly sown, so that the optimal growth period is missed, and the artificial planting of the bulbil polygonum is limited.
Disclosure of Invention
The invention aims to provide a pre-treatment agent before pearl bud seeding and a method for pre-treating pearl buds of polygonum bulbiferum before seeding by using the pre-treatment agent before pearl bud seeding. The pre-treatment agent can relieve dormancy of special pearl buds in alpine regions, thereby fully utilizing the whole suitable growing season to plant the pearl buds.
In one aspect, the invention provides a pre-treatment agent for pre-seeding of a pearl bud, which comprises a first treatment fluid and a second treatment fluid; the first treatment liquid is a copper sulfate aqueous solution, and the second treatment liquid is a plant growth regulator solution. The pre-treatment agent before the pearl bud seeding is used for pre-treating the pearl buds of the polygonum bulbiferum before seeding, so that the germination rate of the pearl buds of the polygonum bulbiferum can be obviously improved.
In some embodiments, the plant growth regulator solution is selected from the group consisting of an kinetin solution, a ethephon solution, a gibberellin solution, a brassin solution, a naphthylacetic acid solution, or any combination thereof.
In other embodiments, the plant growth regulator solution is a mixed aqueous solution of kinetin and ethephon.
In some embodiments, the concentration of kinetin in the mixed aqueous solution of kinetin and ethephon is 20-100 mg/L, and the concentration of ethephon is 50-250 mg/L.
In some embodiments, the mixed aqueous solution of kinetin and ethephon is prepared by dissolving kinetin with 1mol/L hydrochloric acid and mixing with ethephon.
In some embodiments, the first treatment fluid is a 0.4% to 1% aqueous copper sulfate solution. The inventor finds that soaking the bulbels in the aqueous solution at constant temperature is an effective disinfection method, and the germination rate of the bulbels can be obviously improved after the bulbels are treated by the second treatment solution.
In another aspect, the invention provides the application of the pre-treatment agent before the seed of the bulbil in the pre-treatment before the seeding of the bulbil of the polygonum hydropiper.
In another aspect, the invention provides a method for pre-treating polygonum bulbil before sowing by using the pre-treating agent for the polygonum bulbil before sowing, which comprises the following steps:
(1) soaking the pearl buds of the polygonum bulbiferum in the first treatment solution;
(2) soaking the bulblets treated in the step (1) in a second treatment liquid.
Through soaking treatment in the first treatment liquid and soaking treatment in the second treatment liquid, the dormancy characteristic of the polygonum bulborum in alpine mountain areas in harvesting can be relieved, and the sprouting force of the bulborum after dormancy relieving is greatly improved, so that seeding and growth in the whole suitable growing season can be fully utilized.
In some embodiments, the sonication of the bead buds in step (2) is performed simultaneously.
In some embodiments, the beads are washed clean with clear water prior to being soaked in the second pretreatment solution.
In some embodiments, the ultrasound frequency is 30-50kHz, preferably the ultrasound frequency is 40 kHz;
in some embodiments, the bead buds in the step (2) are soaked in the second pretreatment solution for ultrasonic treatment for 2-6 h.
In some embodiments, the soaking time of the polygonum bulbiferum in the step (1) is 1-4 h.
In some embodiments, the soaking temperature is from 30 ℃ to 35 ℃.
In some embodiments, the present invention provides a method for pre-seeding treatment of polygonum bulbiferum bulbil with the pre-seeding treatment agent of the present invention, comprising the steps of:
(1) soaking the polygonum bulbiferum buds in 0.4-1% copper sulfate aqueous solution for 1-4 h at the temperature of 30-35 ℃;
(2) soaking the bulbils treated in the step (1) in a mixed aqueous solution of kinetin and ethephon, and carrying out ultrasonic treatment for 2-6 h, wherein the concentration of kinetin is 20-100 mg/L, and the concentration of ethephon is 50-250 mg/L.
In some embodiments, the mixed aqueous solution of kinetin and ethephon is prepared by dissolving kinetin with 1mol/L hydrochloric acid and mixing with ethephon.
In some embodiments, the beads are washed clean with clear water prior to soaking in the aqueous mixture of kinetin and ethephon.
In some embodiments, the ultrasonic frequency is 30-50kHz, preferably 40 kHz.
In some more preferred embodiments, the present invention provides a method for pre-seeding treatment of polygonum bulbiferum bulbil with the pre-seed-treatment agent of the present invention, comprising the steps of:
(1) soaking Polygonum bulbiferum in 0.6% copper sulfate water solution at 33 deg.C for 2.5 hr;
(2) cleaning the bulbils treated in the step (1) with clear water, soaking in a mixed aqueous solution of kinetin and ethephon, and performing ultrasonic treatment for 2.5h, wherein the concentration of kinetin is 60mg/L, and the concentration of ethephon is 150 mg/L; the mixed aqueous solution of the kinetin and the ethephon is prepared by dissolving the kinetin with 1mol/L hydrochloric acid and then mixing the dissolved kinetin with the ethephon, and the ultrasonic frequency is 40 kHz.
By adopting the technical scheme, the dormancy of the special form of the bulbils in the alpine regions can be released, so that the sprouting and growth of the bulbils are promoted.
The plant growth regulator in the invention is a substance which is artificially synthesized or artificially extracted and can cause the growth and development of plants to change. Common plant growth regulators are diethyl aminoethyl hexanoate (DA-6), forchlorfenuron, sodium nitrophenolate, auxin, brassin, gibberellin, ethylene, ethephon, cytokinin (including but not limited to kinetin), abscisic acid, naphthylacetic acid, brassinolide, salicylic acid, jasmonic acid, paclobutrazol, polyamines, and the like. Preferred plant growth regulators of the invention are kinetin solutions, ethephon solutions, gibberellin solutions, brassin solutions, naphthylacetic acid solutions, or any combination thereof; a more preferred plant growth regulator of the present invention is a mixed aqueous solution of kinetin and ethephon.
The invention has the beneficial effects that:
(1) can relieve the dormancy of the special form of the bulbil in the alpine region, thereby fully utilizing the whole suitable growing season to plant the bulbil polygonum.
(2) Can obviously reduce the rotten rate of the pearl buds and can obviously improve the germination vigor and the germination rate of the pearl buds.
(3) The method for pre-treating the polygonum bulbil before sowing by using the pre-treating agent for the polygonum bulbil before sowing is simple to operate and low in cost.
Drawings
FIG. 1 is a bar graph of mildew rates of the inventive and comparative example bulblets 10d and 21 d.
FIG. 2 is a histogram of the germination rates of the example and comparative example beads 10d and 21d of the present invention.
Detailed Description
The following are preferred embodiments of the present invention, and the present invention is not limited to the following preferred embodiments. It should be noted that various changes and modifications based on the inventive concept herein will occur to those skilled in the art and are intended to be included within the scope of the present invention. The reagents and apparatus used are not indicated by the manufacturer, and are all conventional products commercially available.
The plateau bulbil polygonum bulbil used in the invention is collected in Xining City of Qinghai province, and the plateau bulbil features in the following embodiments: slightly takes the flavor of Chinese herbal medicines, is chocolate brown, is similar to conical bulbil, and has full and unbroken grains.
The term "normal temperature" in the present embodiment means a temperature of 25 to 30 ℃.
Copper sulfate is a reagent commonly used in laboratories, ethephon and kinetin, and is purchased from Shanghai Aladdin Bioagent.
Percent: mass percent, namely mass percent content; mg/L: mg/l; mol/L: mol/l; kHz: kilohertz; DEG C: c, centigrade degree; h: hours; d: days, or days, e.g. 21d means 21 days; mg: mg; mL: and (4) milliliters.
Examples
Example 1 examination of the rotten rate and germination rate of the polygonum bulbil bulbils after treatment with the pre-treatment agent before the bulbil seeding was as follows:
(1) sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) soaking the bulblets in the step (1) in 1% copper sulfate aqueous solution at a constant temperature of 32 ℃, soaking seeds for 4 hours, and fishing out and cleaning with clear water;
(3) seed soaking agent preparation: dissolving 35mg of kinetin by using 1mol/L hydrochloric acid, mixing with 90mg of ethephon, and fixing the volume to 500mL by using pure water;
(4) ultrasonic treatment: immersing the bulblets treated in the step (2) into a compound seed soaking agent, and treating for 3.5 hours at normal temperature by using 40kHz ultrasonic waves;
(5) preparing a paper bed: taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish;
(6) uniformly arranging each part of the bulbels treated in the step (4) on a germinating paper bed (keeping a proper distance between the bulbels and not contacting with each other), adding a proper amount of water, putting the culture dish into a constant-temperature culture box, controlling the temperature to be 25 ℃, and checking every day to keep the paper towels wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Example 2 examination of the rotten rate and germination rate of the polygonum bulbil bulbils treated with the pre-treatment agent before the bulbil seeding was as follows:
(1) sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) soaking the bulblets in the step (1) in a 0.6% copper sulfate aqueous solution at a constant temperature of 33 ℃, soaking seeds for 2.5 hours, taking out and washing with clear water;
(3) seed soaking agent preparation: dissolving 30mg of kinetin by using 1mol/L hydrochloric acid, mixing with 75mg of ethephon, and fixing the volume to 500mL by using pure water;
(4) ultrasonic treatment: immersing the bulbils treated in the step (2) into a compound seed soaking agent, and treating for 4 hours at normal temperature by using 40kHz ultrasonic waves;
(5) preparing a paper bed: taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish;
(6) uniformly arranging each part of the bulbels treated in the step (4) on a germinating paper bed (keeping a proper distance between the bulbels and not contacting with each other), adding a proper amount of water, putting the culture dish into a constant-temperature culture box, controlling the temperature to be 25 ℃, and checking every day to keep the paper towels wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Example 3 examination of the rotten rate and germination rate of the polygonum bulbil bulbils treated with the pre-treatment agent before the bulbil seeding was as follows:
(1) sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) soaking the bulblets in the step (1) in a 0.8% copper sulfate aqueous solution at a constant temperature of 35 ℃ for 4 hours, and fishing out and cleaning with clear water;
(3) seed soaking agent preparation: dissolving 50mg of kinetin by using 1mol/L hydrochloric acid, mixing with 50mg of ethephon, and fixing the volume to 500mL by using pure water;
(4) ultrasonic treatment: immersing the bulbils treated in the step (2) into a compound seed soaking agent, and treating for 5 hours at normal temperature by using 40kHz ultrasonic waves;
(5) preparing a paper bed: taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish;
(6) uniformly arranging each part of the bulbels treated in the step (4) on a germinating paper bed (keeping a proper distance between the bulbels and not contacting with each other), adding a proper amount of water, putting the culture dish into a constant-temperature culture box, controlling the temperature to be 25 ℃, and checking every day to keep the paper towels wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Example 4 examination of the rotten rate and germination rate of the polygonum bulbil bulbils treated with the pre-treatment agent before the bulbil seeding was as follows:
(1) sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) soaking the bulblets in the step (1) in a 0.5% copper sulfate aqueous solution at a constant temperature of 34 ℃, soaking seeds for 3 hours, taking out and washing with clear water;
(3) seed soaking agent preparation: dissolving 45mg of kinetin by using 1mol/L hydrochloric acid, mixing with 75mg of ethephon, and fixing the volume to 500mL by using pure water;
(4) ultrasonic treatment: immersing the bulbils treated in the step (2) into a compound seed soaking agent, and treating for 3 hours at normal temperature by using 40kHz ultrasonic waves;
(5) preparing a paper bed: taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish;
(6) uniformly arranging each part of the bulbels treated in the step (4) on a germinating paper bed (keeping a proper distance between the bulbels and not contacting with each other), adding a proper amount of water, putting the culture dish into a constant-temperature culture box, controlling the temperature to be 25 ℃, and checking every day to keep the paper towels wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Example 5 examination of the rotten rate and germination rate of the polygonum bulbil bulbils treated with the pre-treatment agent before the bulbil seeding was as follows:
(1) sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) soaking the bulblets in the step (1) in a 0.5% copper sulfate aqueous solution at a constant temperature of 33 ℃, soaking seeds for 2 hours, taking out and washing with clear water;
(3) seed soaking agent preparation: dissolving 50mg of kinetin by using 1mol/L hydrochloric acid, mixing with 125mg of ethephon, and fixing the volume to 500mL by using pure water;
(4) ultrasonic treatment: immersing the bulblets treated in the step (2) into a compound seed soaking agent, and treating for 2.5 hours at normal temperature by using 40kHz ultrasonic waves;
(5) preparing a paper bed: taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish;
(6) uniformly arranging the treated bulbils on a germinating paper bed (keeping a proper distance between the bulbils and not contacting with each other), adding a proper amount of water, placing the culture dish into a constant-temperature incubator, controlling the temperature to be 25 ℃, and checking every day to keep the paper towel wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Comparative example 1 investigation of the rotten rate and germination rate of Polygonum criopolitanum Pearl buds without any treatment
(1) Sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) taking a clean and disinfected culture dish without any treatment, and putting a moisture absorption paper towel into the culture dish; uniformly arranging the above bulbils on a germinating paper bed (keeping a proper distance between the bulbils without mutual contact), adding a proper amount of water, placing the culture dish into a constant-temperature incubator, controlling the temperature at 25 ℃, and checking every day to keep the paper towel wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Comparative example 2 investigation of the rotten rate and germination rate of Polygonum criopolitanum Georgi treated with a Mixed aqueous solution of kinetin and ethephon
(1) Sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) seed soaking agent preparation: dissolving 50mg of kinetin by using 1mol/L hydrochloric acid, mixing with 125mg of ethephon, and fixing the volume to 500mL by using pure water;
(3) treating the bulbil in the step (1) for 6 hours at normal temperature by using the seed soaking agent 40kHz ultrasonic wave;
taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish; uniformly arranging the above bulbils on a germinating paper bed (keeping a proper distance between the bulbils without mutual contact), adding a proper amount of water, placing the culture dish into a constant-temperature incubator, controlling the temperature at 25 ℃, and checking every day to keep the paper towel wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Comparative example 3 investigation of rotten rate and germination rate of Polygonum bulbiferum after ultrasonic immersion treatment with pure Water
(1) Sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) immersing the bulbil in the step (1) in pure water, and treating for 5.5h at normal temperature by using 40kHz ultrasonic waves;
taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish; uniformly arranging the above bulbils on a germinating paper bed (keeping a proper distance between the bulbils without mutual contact), adding a proper amount of water, placing the culture dish into a constant-temperature incubator, controlling the temperature at 25 ℃, and checking every day to keep the paper towel wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Comparative example 4 examination of the rotten percentage and the germination percentage of Polygonum bulbiferum after soaking in copper sulfate aqueous solution
(1) Sampling bulbil: randomly selecting 3 parts of full and undamaged plateau bulbels of cleaned particles, wherein 200 particles are selected;
(2) immersing the bulbels in the step (1) in 1% copper sulfate solution at the immersion temperature of 30 ℃ for 3 h;
taking a clean and disinfected culture dish, and putting a moisture absorption paper towel into the culture dish; uniformly arranging the above bulbils on a germinating paper bed (keeping a proper distance between the bulbils without mutual contact), adding a proper amount of water, placing the culture dish into a constant-temperature incubator, controlling the temperature at 25 ℃, and checking every day to keep the paper towel wet.
The rotten rate of the bulbil is shown in Table 1 and attached figure 1, and the germination vigor and germination rate of the bulbil are shown in Table 2 and attached figure 2.
Examples of the experiments
The degree of dormancy breaking was evaluated for the beads of 5 treated groups in examples 1 to 5 and 4 control groups in comparative examples 1 to 4 as follows.
1. The evaluation method comprises the following steps:
(1) rate of rotten pearl buds
As the germination time of the bulbels is prolonged in the germination process, the bulbels can mildew and rot, and the lower the mildew number is, the higher the quality of the bulbels is. And (4) recording the mildewing condition of the sprouting period of the bead buds at 10d and 21d, if only the surface of the bead buds mildews, treating the surface of the bead buds, returning the treated surface of the bead buds to a germinating bed, and if the interior of the bead buds are rotted, removing the surface of the bead buds and recording the surface of the bead buds. The mildew rate was calculated according to the following formula and reported as the average of three replicates.
(2) Germination potential and germination rate of pearl buds
Identification standard of bud germination: 1/3, the length of the buds of which at least reaches the length of the bulbels, is the normal germination, the percentage of the germinated bulbels within 10 days under the culture condition of 25 ℃ is taken as the germination potential of the bulbels, and the germination percentage of the bulbels under the same condition of 21 days is taken as the germination rate of the bulbels. The results of observation and recording were used to calculate the germination potential and germination percentage of each group of beads according to the following formulas, and the recorded values were the average of three replicates.
2. Results and analysis
The pearl bud rot rates of examples 1 to 5 and comparative examples 1 to 4 are shown in Table 1 and FIG. 1; the germination rates of the beads of examples 1 to 5 and comparative examples 1 to 4 are shown in Table 2 and FIG. 2.
TABLE 1 mushrooming during sprouting of the bulblets
As can be seen from Table 1 or FIG. 1, the highest mildewing rate of the pearl buds of the comparative example 1 can reach 78.19 percent, and the mildewing rates of the pearl buds of the comparative examples 2 to 4 are more than 45 percent; the mildewing rate of the pearl buds in the examples 1 to 5 is less than 26 percent, wherein the mildewing rate of the pearl buds in the example 2 is 20.44 percent at the lowest. The treatment methods of the example 1-5 have obvious effect on preventing the pearl buds from mildewing and softening, and the effect is better than that of the comparative example 1-4, which shows that the pearl buds treated by the processes of sterilization, seed soaking agent compounding, ultrasonic treatment and the like can effectively prevent the pearl buds from mildewing and softening, and the effect of the example 1 and the example 2 is optimal.
TABLE 2 germination vigor and germination rate of the pearl buds
As can be seen from Table 2 or FIG. 2, the germination potentials of comparative examples 1-4 were all less than 25%, those of examples 1-2, 4-5 were more than 30%, and those of example 3 were 29.85%. Accordingly, the germination rates of comparative examples 1-4 were less than 40%, wherein the germination rate of comparative example 1 was 17.81% at the lowest; the germination rates of the examples 1-5 are all higher than 60%, wherein the germination rate of the example 2 is up to 71.78%, which is about 4 times that of the comparative example 1.
In conclusion, the method for treating the bulbil can relieve the dormancy of the bulbil with the special form in the plateau, is obviously superior to untreated bulbil, ensures that the germination rate of the bulbil reaches more than 60 percent, can provide various seed sources for the artificial planting of the bulbil polygonum and can fully utilize the whole suitable growing season.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (10)
1. The pre-treatment agent before the pearl bud seeding is characterized by comprising a first treatment fluid and a second treatment fluid; the first treatment liquid is a copper sulfate aqueous solution, and the second treatment liquid is a plant growth regulator solution.
2. The pre-treatment agent before pearl bud seeding according to claim 1, wherein the plant growth regulator solution is selected from kinetin solution, ethephon solution, gibberellin solution, brassin solution, naphthylacetic acid solution, or any combination thereof;
preferably, the plant growth regulator solution is a mixed aqueous solution of kinetin and ethephon;
more preferably, the concentration of the kinetin in the mixed water solution of kinetin and ethephon is 20-100 mg/L, and the concentration of ethephon is 50-250 mg/L;
more preferably, the mixed aqueous solution of the kinetin and the ethephon is prepared by dissolving kinetin with 1mol/L hydrochloric acid and mixing with ethephon.
3. The pre-treatment agent before the pearl bud seeding as claimed in claim 1, wherein the first treatment fluid is 0.4% -1% copper sulfate aqueous solution.
4. Use of the pre-treatment agent for pearl buds as claimed in any one of claims 1 to 3 in pre-treatment of pearl buds of Polygonum hydropiper before sowing.
5. A method for pre-treating the polygonum bulbil before sowing by using the pre-seed pre-treating agent of any one of claims 1 to 3, which is characterized by comprising the following steps:
(1) soaking the pearl buds of the polygonum bulbiferum in the first treatment solution;
(2) and (3) soaking the bulbels treated in the step (1) in a second pretreatment solution.
6. The method according to claim 5, wherein the ultrasonic treatment is simultaneously performed on the bead buds in the step (2);
optionally, the bead buds are washed clean with clear water before being soaked in the second pretreatment solution.
7. The method according to claim 6, characterized in that the ultrasonic frequency is 30-50kHz, preferably 40 kHz;
optionally, the bead buds in the step (2) are soaked in the second treatment liquid for ultrasonic treatment for 2-6 h.
8. The method according to claim 5, wherein in the step (1), the soaking time of the polygonum bulbiferum is 1-4 h; optionally, the soaking temperature is 30-35 ℃.
9. The method of claim 5, comprising the steps of:
(1) soaking the polygonum bulbiferum buds in 0.4-1% copper sulfate aqueous solution for 1-4 h at the temperature of 30-35 ℃;
(2) soaking the bulbils treated in the step (1) in a mixed aqueous solution of kinetin and ethephon, and carrying out ultrasonic treatment for 2-6 h, wherein the concentration of kinetin is 20-100 mg/L, and the concentration of ethephon is 50-250 mg/L.
10. Method according to claim 9, characterized in that the ultrasonic frequency is 30-50kHz, preferably 40 kHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011261266.3A CN112314625A (en) | 2020-11-12 | 2020-11-12 | Pre-seeding pretreatment agent for bulbil and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011261266.3A CN112314625A (en) | 2020-11-12 | 2020-11-12 | Pre-seeding pretreatment agent for bulbil and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112314625A true CN112314625A (en) | 2021-02-05 |
Family
ID=74317297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011261266.3A Pending CN112314625A (en) | 2020-11-12 | 2020-11-12 | Pre-seeding pretreatment agent for bulbil and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112314625A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434500A (en) * | 2008-11-28 | 2009-05-20 | 浙江林学院 | Fertilizer containing plant charcoals and preparation thereof |
| CN105103711A (en) * | 2015-08-19 | 2015-12-02 | 单县绿丰种业有限公司 | Germination method for tomato seeds |
| CN105766136A (en) * | 2016-03-10 | 2016-07-20 | 中国农业科学院特产研究所 | Method for increasing germination rate of seed of schisandra chinensis |
| CN109511677A (en) * | 2017-09-20 | 2019-03-26 | 龙里县柯臻科技种植有限公司 | A kind of sponge gourd seed-soaking liquid and preparation method thereof |
| CN109734498A (en) * | 2019-03-04 | 2019-05-10 | 西南大学 | A kind of serpentgrass water planting nutrient solution and serpentgrass ciltivating process |
| CN110012827A (en) * | 2019-03-20 | 2019-07-16 | 广东东阳光药业有限公司 | A kind of serpentgrass method for culturing seedlings |
| CN110199787A (en) * | 2019-05-21 | 2019-09-06 | 广东东阳光药业有限公司 | It is a kind of while regulating and controlling serpentgrass and bloom and bead up the method for bud |
| CN110999914A (en) * | 2019-11-29 | 2020-04-14 | 东莞市东阳光冬虫夏草中药有限公司 | Chinese medicinal material seed pretreatment agent and application thereof |
| CN111134123A (en) * | 2019-12-19 | 2020-05-12 | 东莞市东阳光冬虫夏草中药有限公司 | Seed germination treatment agent and application |
| CN111296413A (en) * | 2019-11-28 | 2020-06-19 | 东莞市东阳光冬虫夏草中药有限公司 | Preservative, application and method for preserving polygonum bulbiferum bulbils by using preservative |
-
2020
- 2020-11-12 CN CN202011261266.3A patent/CN112314625A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434500A (en) * | 2008-11-28 | 2009-05-20 | 浙江林学院 | Fertilizer containing plant charcoals and preparation thereof |
| CN105103711A (en) * | 2015-08-19 | 2015-12-02 | 单县绿丰种业有限公司 | Germination method for tomato seeds |
| CN105766136A (en) * | 2016-03-10 | 2016-07-20 | 中国农业科学院特产研究所 | Method for increasing germination rate of seed of schisandra chinensis |
| CN109511677A (en) * | 2017-09-20 | 2019-03-26 | 龙里县柯臻科技种植有限公司 | A kind of sponge gourd seed-soaking liquid and preparation method thereof |
| CN109734498A (en) * | 2019-03-04 | 2019-05-10 | 西南大学 | A kind of serpentgrass water planting nutrient solution and serpentgrass ciltivating process |
| CN110012827A (en) * | 2019-03-20 | 2019-07-16 | 广东东阳光药业有限公司 | A kind of serpentgrass method for culturing seedlings |
| CN110199787A (en) * | 2019-05-21 | 2019-09-06 | 广东东阳光药业有限公司 | It is a kind of while regulating and controlling serpentgrass and bloom and bead up the method for bud |
| CN111296413A (en) * | 2019-11-28 | 2020-06-19 | 东莞市东阳光冬虫夏草中药有限公司 | Preservative, application and method for preserving polygonum bulbiferum bulbils by using preservative |
| CN110999914A (en) * | 2019-11-29 | 2020-04-14 | 东莞市东阳光冬虫夏草中药有限公司 | Chinese medicinal material seed pretreatment agent and application thereof |
| CN111134123A (en) * | 2019-12-19 | 2020-05-12 | 东莞市东阳光冬虫夏草中药有限公司 | Seed germination treatment agent and application |
Non-Patent Citations (2)
| Title |
|---|
| 《种子工作手册》编写组编: "《种子工作手册》", 30 June 1979, 上海科学技术出版社 * |
| 邹园: "珠芽蓼的开花结实和胎生繁殖", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105009877B (en) | A kind of high yield of spinach and selfing are reserved seed for planting method | |
| CN106688849A (en) | Soilless culture method of Chinese flowering cabbage | |
| CN107235803A (en) | A kind of high-yield planting base manure of guava and preparation method thereof | |
| CN109315247B (en) | Method for producing selenium-rich rice by using astragalus sinicus and rape activated soil | |
| CN114097829B (en) | Fungal metabolite dry powder for promoting plant growth, fungal metabolite active substance, preparation method and application thereof | |
| CN106817955A (en) | A kind of processing method of corn seed | |
| CN102899266A (en) | Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and application | |
| CN106613832A (en) | Culture method of dendrobium officinale | |
| CN104145559B (en) | A kind of method making watermelon germination neat and consistent | |
| CN107852886A (en) | A kind of method for promoting swordleaf dragon tree seed to sprout | |
| CN104620718A (en) | Method for breaking dormancy of digitaria sanguinalis seeds | |
| CN113854090B (en) | Seedling raising method for improving perennial root performance of sugarcane | |
| CN113349227B (en) | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method | |
| WO2012116475A1 (en) | Seedling-raising and root-shaping agent for inserting into plate of rice seeding machine and application method thereof | |
| CN109729780A (en) | A method of improving germination of stevia rebaudiana seeds rate | |
| CN109526298A (en) | A kind of passion fruit method for treating seeds and its seed propagation method | |
| CN112314625A (en) | Pre-seeding pretreatment agent for bulbil and application thereof | |
| CN113892425A (en) | Rapid cultivation method of hazelnut semi-aseptic seedlings | |
| Menesy et al. | Response of Pimpinella anisum L plant for graphite and silica nanoparticles | |
| CN107466529A (en) | A kind of method for culturing seedlings for improving water spinach resistance | |
| CN107371449A (en) | A kind of storage method of calophyllum inophyllum seed | |
| CN112806260A (en) | Tissue culture seedling raising method for baby cabbage with high disease resistance | |
| CN111480560A (en) | Soilless rice seedling raising method | |
| CN108323273B (en) | Formula solution and method for rapidly relieving dormancy of wild bitter gourd seeds and application | |
| CN113796311B (en) | Method for inducing tetraploid of phellodendron amurense |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210205 |