CN107371449A - A kind of storage method of calophyllum inophyllum seed - Google Patents
A kind of storage method of calophyllum inophyllum seed Download PDFInfo
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- CN107371449A CN107371449A CN201710470236.5A CN201710470236A CN107371449A CN 107371449 A CN107371449 A CN 107371449A CN 201710470236 A CN201710470236 A CN 201710470236A CN 107371449 A CN107371449 A CN 107371449A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/18—Mercury
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
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Abstract
The present invention relates to a kind of storage method of calophyllum inophyllum seed, following steps are specifically included：（1）The harvesting of calophyllum inophyllum and storage；（2）The processing of seed；（3）The sowing of seed.By experiment, a kind of storage method of calophyllum inophyllum seed provided by the invention, the calophyllum inophyllum percentage of seedgermination than conventional method storage is higher, and germination percentage is promoted to more than 79.0% by the 68.4% of routine, significant effect, is worth of widely use.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of storage method of calophyllum inophyllum seed.
Background technology
Calophyllum inophyllum, the fruit of cherry-apple tree, Parietales, rose family arbor, bark is thick, taupe or crineous, has vertical
Crack, site of injury often ooze out transparent resin；Pericarp color is scarlet brilliant, pulp yellow-white, and fruity is strong fragrant, eats sour-sweet delicious and crisp raw.
Fruit oil be available for it is industrial, processing detoxification and refining after edible, also be available for pharmaceutical；Cherry-apple tree timber quality is solid, heavier,
It wear-resistant can be soaked with seawater, food of not damaged by worms, be suitable for shipbuilding, bridge, sleeper, farm implements and furniture etc. and use material；Bark is containing single
Peaceful 15%, tannin extract can be obtained through refining.
In the extensive seedling raising process of Malus spectabilis, the method sowed using calophyllum inophyllum seed is tasted, but calophyllum inophyllum seed passes through
Passed the winter using prior art storage, embryo activity is easily reduced, and causes percentage of seedgermination not high, has a strong impact on cultivation progress.
The content of the invention
In order to solve the above problems, the invention provides a kind of storage method of calophyllum inophyllum seed.
The present invention is achieved through the following technical solutions：
A kind of storage method of calophyllum inophyllum seed, specifically includes following steps：
（1）The harvesting of calophyllum inophyllum and storage
Annual late September to early October, selection whole year grow fine, the disease-free maternal plant of health, harvest the calophyllum inophyllum of maturation,
Using 50-55 DEG C of hot air circulation drying to fruit water content below 5%, then using ultraviolet sterilizing lamp treatment with irradiation 15 minutes,
Finally compound preservative, fountain height 100ml/kg are sprayed in fruit surface；
The calophyllum inophyllum handled well is installed using the polybag totally sterilized, then uses -15 DEG C of cryogenic freezing 20-25 minutes,
A calophyllum inophyllum was stirred during freezing every 3 minutes, ensures calophyllum inophyllum lowering temperature uniform, then tightens polybag, by calophyllum inophyllum
It is positioned in 0-2 DEG C of light protected environment and stores；
（2）The processing of seed
In the Second Year beginning of spring, calophyllum inophyllum is taken out, wash away pulp, pericarp obtains calophyllum inophyllum seed, then seed eluriated using clear water dry
Only, method is eluriated clockwise using 25-30 DEG C of warm water during elutriation, avoid injury kind skin and embryo, after the completion of elutriation, seed is made
With 35-40kHz ultrasonications 20 minutes, strengthen absorption of the seed to decoction, it is then that seed is small using decoction immersion 4-5
When, recover seed cell metabolism degree, abolish seed dormancy phase, ensure the germination percentage of seed；The decoction, its preparation side
Method, comprise the following steps：
A takes cinnabar 2-4 parts, mother-of-pearl 2-4 parts, shell 5-8 parts to grind, and adds the water of 20 times of shell mass fractions fully to stir
Mix, until impurity is not redissolved, stands, take supernatant liquor to obtain solution A, can help to improve the respiratory rate of kind of blastocyte, protect
Demonstrate,prove the development of plumule；
B takes polygala 5-6 parts, cortex albiziae 2-3 parts, orange peel 2-3 parts, is fully ground and mashes after cleaning, and filters to get filtrate and filter residue, so
The juice in remaining filter residue is extruded afterwards, after the filtrate and juice are mixed plus 20 times of water dilutes to obtain solution B, can be effective
Promote absorption of the seed to active ingredient in decoction, while further abolish seed dormancy phase, the metabolism for improving seed cell is strong
Degree；
C is by the solution A, solution B in mass ratio 2:The decoction is produced after 1 mixing；
（3）The sowing of seed
By the seed handled well using can be sowed after auxin solution vernalization.
Beneficial effects of the present invention：A kind of storage method of calophyllum inophyllum seed provided by the invention, the directly sea to maturation
Chinese bush cherry fruit fruit is handled, and is first dried calophyllum inophyllum and is used ultraviolet disinfection again, kills fruit surface and internal bacterial micro-organism, finally
Compound preservative is sprayed in fruit surface, prevents calophyllum inophyllum goes mouldy from being grown with germ, and uses compound preservative processing Malus spectabilis
Fruit, the inhibitory action sprouted using single preservative to seed in fruit is avoided, be effectively guaranteed the activity of embryo；Then
It will directly be refrigerated after the processing of calophyllum inophyllum cryogenic freezing, due to directly freezed calophyllum inophyllum, lower temperature can be born, be unlikely to hinder
Evil is to internal seed, and seed is in calophyllum inophyllum and good storage ambient is provided for seed, effective guarantee seed
Activity, improve the germination percentage of seed；Seed is used ultrasonication by the Second Year beginning of spring, is then soaked using decoction, strengthens kind
Absorption of the son to decoction, the active ingredient in the decoction can be recovered seed cell metabolism degree, abolish seed dormancy phase,
Ensure the germination percentage of seed.By experiment, a kind of storage method of calophyllum inophyllum seed provided by the invention, stored than conventional method
Calophyllum inophyllum percentage of seedgermination it is higher, germination percentage 68.4% is promoted to more than 79.0% by routine, and significant effect, being worthy to be popularized makes
With.
Embodiment
Embodiment 1
A kind of storage method of calophyllum inophyllum seed, specifically includes following steps：
（1）The harvesting of calophyllum inophyllum and storage
Annual late September to early October, selection whole year grow fine, the disease-free maternal plant of health, harvest the calophyllum inophyllum of maturation,
Using 50-55 DEG C of hot air circulation drying to fruit water content below 5%, then using ultraviolet sterilizing lamp treatment with irradiation 15 minutes,
Finally compound preservative, fountain height 100ml/kg are sprayed in fruit surface；
The calophyllum inophyllum handled well is installed using the polybag totally sterilized, then uses -15 DEG C of cryogenic freezing 20-25 minutes,
A calophyllum inophyllum was stirred during freezing every 3 minutes, ensures calophyllum inophyllum lowering temperature uniform, then tightens polybag, by calophyllum inophyllum
It is positioned in 0-2 DEG C of light protected environment and stores；
（2）The processing of seed
In the Second Year beginning of spring, calophyllum inophyllum is taken out, wash away pulp, pericarp obtains calophyllum inophyllum seed, then seed eluriated using clear water dry
Only, method is eluriated clockwise using 25-30 DEG C of warm water during elutriation, avoid injury kind skin and embryo, after the completion of elutriation, seed is made
With 35-40kHz ultrasonications 20 minutes, strengthen absorption of the seed to decoction, it is then that seed is small using decoction immersion 4-5
When, recover seed cell metabolism degree, abolish seed dormancy phase, ensure the germination percentage of seed；The decoction, its preparation side
Method, comprise the following steps：
A takes 2 parts of cinnabar, 2 parts of mother-of-pearl, 5 parts of shell to grind, and adds the water of 20 times of shell mass fractions to be sufficiently stirred, until
Impurity is not redissolved, and is stood, is taken supernatant liquor to obtain solution A, can help to improve the respiratory rate of kind of blastocyte, ensure plumule
Development；
B takes 5 parts of polygala, 2 parts of cortex albiziae, 2 parts of orange peel, is fully ground and mashes after cleaning, and filters to get filtrate and filter residue, then will be surplus
Under filter residue in juice extrusion, will the filtrate and juice mix after plus 20 times of water dilute to obtain solution B, kind can be effectively facilitated
Absorption of the son to active ingredient in decoction, while seed dormancy phase is further abolished, improve the metabolism degree of seed cell；
C is by the solution A, solution B in mass ratio 2:The decoction is produced after 1 mixing；
（3）The sowing of seed
By the seed handled well using can be sowed after auxin solution vernalization.
Embodiment 2
A kind of storage method of calophyllum inophyllum seed, specifically includes following steps：
（1）The harvesting of calophyllum inophyllum and storage
Annual late September to early October, selection whole year grow fine, the disease-free maternal plant of health, harvest the calophyllum inophyllum of maturation,
Using 50-55 DEG C of hot air circulation drying to fruit water content below 5%, then using ultraviolet sterilizing lamp treatment with irradiation 15 minutes,
Finally compound preservative, fountain height 100ml/kg are sprayed in fruit surface；
The calophyllum inophyllum handled well is installed using the polybag totally sterilized, then uses -15 DEG C of cryogenic freezing 20-25 minutes,
A calophyllum inophyllum was stirred during freezing every 3 minutes, ensures calophyllum inophyllum lowering temperature uniform, then tightens polybag, by calophyllum inophyllum
It is positioned in 0-2 DEG C of light protected environment and stores；
（2）The processing of seed
In the Second Year beginning of spring, calophyllum inophyllum is taken out, wash away pulp, pericarp obtains calophyllum inophyllum seed, then seed eluriated using clear water dry
Only, method is eluriated clockwise using 25-30 DEG C of warm water during elutriation, avoid injury kind skin and embryo, after the completion of elutriation, seed is made
With 35-40kHz ultrasonications 20 minutes, strengthen absorption of the seed to decoction, it is then that seed is small using decoction immersion 4-5
When, recover seed cell metabolism degree, abolish seed dormancy phase, ensure the germination percentage of seed；The decoction, its preparation side
Method, comprise the following steps：
A takes 4 parts of cinnabar, 4 parts of mother-of-pearl, 8 parts of shell to grind, and adds the water of 20 times of shell mass fractions to be sufficiently stirred, until
Impurity is not redissolved, and is stood, is taken supernatant liquor to obtain solution A, can help to improve the respiratory rate of kind of blastocyte, ensure plumule
Development；
B takes 6 parts of polygala, 3 parts of cortex albiziae, 3 parts of orange peel, is fully ground and mashes after cleaning, and filters to get filtrate and filter residue, then will be surplus
Under filter residue in juice extrusion, will the filtrate and juice mix after plus 20 times of water dilute to obtain solution B, kind can be effectively facilitated
Absorption of the son to active ingredient in decoction, while seed dormancy phase is further abolished, improve the metabolism degree of seed cell；
C is by the solution A, solution B in mass ratio 2:The decoction is produced after 1 mixing；
（3）The sowing of seed
By the seed handled well using can be sowed after auxin solution vernalization.
Comparative example 1
This comparative example 1 compared with Example 1, without step（1）The operation, but seed refrigeration is directly harvested, remove
In addition method and step all same.
Comparative example 2
This comparative example 2 compared with Example 2, without step（2）The operation, seed is handled using only conventional method,
Method and step all same in addition.
Control group
Conventional calophyllum inophyllum seed storage method.
Stored respectively using above-mentioned 5 kinds of methods to same kind, with a batch of calophyllum inophyllum seed, then will storage
The seed sowing crossed, count its germination percentage, many experiments, as a result such as table 1：
Table 1
| Sow number（Grain） | Germinative number（Grain） | Germination percentage（%） | |
| Embodiment 1 | 300 | 258 | 86.0 |
| Embodiment 2 | 300 | 262 | 87.3 |
| Comparative example 1 | 300 | 221 | 73.7 |
| Comparative example 2 | 300 | 217 | 72.3 |
| Control group | 300 | 236 | 78.7 |
As shown in Table 1, the storage method of a kind of calophyllum inophyllum seed provided by the invention, than the calophyllum inophyllum seed of conventional method storage
Germination percentage is higher, and germination percentage is promoted to more than 86.0% by the 78.7% of prior art, significant effect, is worth of widely use.
Claims (2)
1. a kind of storage method of calophyllum inophyllum seed, it is characterised in that specifically include following steps：
（1）The harvesting of calophyllum inophyllum and storage
Annual late September to early October, selection whole year grow fine, the disease-free maternal plant of health, harvest the calophyllum inophyllum of maturation,
Using 50-55 DEG C of hot air circulation drying to fruit water content below 5%, then using ultraviolet sterilizing lamp treatment with irradiation 15 minutes,
Finally compound preservative, fountain height 100ml/kg are sprayed in fruit surface；
The calophyllum inophyllum handled well is installed using the polybag totally sterilized, then uses -15 DEG C of cryogenic freezing 20-25 minutes,
A calophyllum inophyllum was stirred during freezing every 3 minutes, ensures calophyllum inophyllum lowering temperature uniform, then tightens polybag, by calophyllum inophyllum
It is positioned in 0-2 DEG C of light protected environment and stores；
（2）The processing of seed
In the Second Year beginning of spring, calophyllum inophyllum is taken out, wash away pulp, pericarp obtains calophyllum inophyllum seed, then seed eluriated using clear water dry
Only, method is eluriated clockwise using 25-30 DEG C of warm water during elutriation, avoid injury kind skin and embryo, after the completion of elutriation, seed is made
With 35-40kHz ultrasonications 20 minutes, seed is then used into decoction immersion 4-5 hours；The decoction, its preparation method,
Comprise the following steps：
A takes cinnabar 2-4 parts, mother-of-pearl 2-4 parts, shell 5-8 parts to grind, and adds the water of 20 times of shell mass fractions fully to stir
Mix, until impurity is not redissolved, stands, take supernatant liquor to obtain solution A；
B takes polygala 5-6 parts, cortex albiziae 2-3 parts, orange peel 2-3 parts, is fully ground and mashes after cleaning, and filters to get filtrate and filter residue, so
The juice in remaining filter residue is extruded afterwards, after the filtrate and juice are mixed plus 20 times of water dilutes to obtain solution B；
C is by the solution A, solution B in mass ratio 2:The decoction is produced after 1 mixing；
（3）The sowing of seed
By the seed handled well using can be sowed after auxin solution vernalization.
A kind of 2. storage method of calophyllum inophyllum seed according to claim 1, it is characterised in that step（2）The decoction,
Its preparation method, comprise the following steps：
A takes 4 parts of cinnabar, 4 parts of mother-of-pearl, 8 parts of shell to grind, and adds the water of 20 times of shell mass fractions to be sufficiently stirred, until
Impurity is not redissolved, and is stood, is taken supernatant liquor to obtain solution A；
B takes 6 parts of polygala, 3 parts of cortex albiziae, 3 parts of orange peel, is fully ground and mashes after cleaning, and filters to get filtrate and filter residue, then will be surplus
Under filter residue in juice extrusion, will the filtrate and juice mix after plus 20 times of water dilute to obtain solution B；
C is by the solution A, solution B in mass ratio 2:The decoction is produced after 1 mixing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710470236.5A CN107371449A (en) | 2017-06-20 | 2017-06-20 | A kind of storage method of calophyllum inophyllum seed |
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| CN201710470236.5A CN107371449A (en) | 2017-06-20 | 2017-06-20 | A kind of storage method of calophyllum inophyllum seed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111819947A (en) * | 2020-07-17 | 2020-10-27 | 新疆林业科学院 | Germination accelerating method beneficial to dormancy breaking of sorbus tianshanensis seeds |
| CN112514751A (en) * | 2020-11-30 | 2021-03-19 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532347A (en) * | 2015-12-07 | 2016-05-04 | 烟台大学 | Method for reducing virus-carrying rate of Malus Robusta seed and improving germination rate |
| CN106258072A (en) * | 2016-08-12 | 2017-01-04 | 广西壮族自治区药用植物园 | The method improving hypericum ascyron seed germination rate |
| CN106577135A (en) * | 2016-12-19 | 2017-04-26 | 重庆市中药研究院 | Tripterygium hypoglaucum seed propagation method |
-
2017
- 2017-06-20 CN CN201710470236.5A patent/CN107371449A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532347A (en) * | 2015-12-07 | 2016-05-04 | 烟台大学 | Method for reducing virus-carrying rate of Malus Robusta seed and improving germination rate |
| CN106258072A (en) * | 2016-08-12 | 2017-01-04 | 广西壮族自治区药用植物园 | The method improving hypericum ascyron seed germination rate |
| CN106577135A (en) * | 2016-12-19 | 2017-04-26 | 重庆市中药研究院 | Tripterygium hypoglaucum seed propagation method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111819947A (en) * | 2020-07-17 | 2020-10-27 | 新疆林业科学院 | Germination accelerating method beneficial to dormancy breaking of sorbus tianshanensis seeds |
| CN112514751A (en) * | 2020-11-30 | 2021-03-19 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
| CN112514751B (en) * | 2020-11-30 | 2022-06-17 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
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