CN108949944A - 产生用于纳米孔传感器的双层的方法 - Google Patents
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| EP3415901A1 (en) * | 2012-01-20 | 2018-12-19 | Genia Technologies, Inc. | Nanopore based molecular detection and sequencing |
| EP2814983B1 (en) | 2012-02-16 | 2019-04-24 | Genia Technologies, Inc. | Methods for creating bilayers for use with nanopore sensors |
| WO2013154999A2 (en) | 2012-04-09 | 2013-10-17 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
| GB2510719A (en) * | 2012-06-15 | 2014-08-13 | Genia Technologies Inc | Chip set-up and high-accuracy nucleic acid sequencing |
| EP2864502B1 (en) * | 2012-06-20 | 2019-10-23 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| CN105102627B (zh) | 2013-03-15 | 2018-10-19 | 纽约哥伦比亚大学理事会 | 用于检测样品中多种预定化合物的方法 |
| KR102245192B1 (ko) * | 2013-05-06 | 2021-04-29 | 온테라 인크. | 나노포어를 이용한 표적 검출 |
| US20140329225A1 (en) | 2013-05-06 | 2014-11-06 | Two Pore Guys, Inc. | Target detection with nanopore |
| WO2014190299A2 (en) * | 2013-05-23 | 2014-11-27 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Chemistry, systems and methods of translocation of a polymer through a nanopore |
| CA2926138A1 (en) | 2013-10-23 | 2015-04-30 | Genia Technologies, Inc. | High speed molecular sensing with nanopores |
| US9322062B2 (en) | 2013-10-23 | 2016-04-26 | Genia Technologies, Inc. | Process for biosensor well formation |
| US10240195B2 (en) | 2014-03-24 | 2019-03-26 | The Trustees Of Columbia University In The City Of New York | Chemical methods for producing tagged nucleotides |
| EP3683318A1 (en) * | 2014-06-03 | 2020-07-22 | Illumina, Inc. | Compositions, systems, and methods for detecting events using tethers anchored to or adjacent to nanopores |
| CA3163156C (en) * | 2014-07-31 | 2025-07-08 | Illumina Inc | HYBRID NANOPORE SENSORS |
| ES2774802T3 (es) | 2014-10-31 | 2020-07-22 | Genia Tech Inc | Variantes de hemolisina alfa con características alteradas |
| US10036739B2 (en) | 2015-01-27 | 2018-07-31 | Genia Technologies, Inc. | Adjustable bilayer capacitance structure for biomedical devices |
| CN104651500B (zh) * | 2015-01-30 | 2017-06-30 | 华东理工大学 | 气单胞菌溶素纳米孔通道的制备方法及其应用 |
| CN107257854B (zh) | 2015-02-02 | 2022-02-15 | 豪夫迈·罗氏有限公司 | 聚合酶变体 |
| US9791432B2 (en) | 2015-03-20 | 2017-10-17 | Genia Technologies, Inc. | Serpentine flow channels for flowing fluids over chip sensors |
| TWI571626B (zh) * | 2015-07-15 | 2017-02-21 | 力晶科技股份有限公司 | 具有奈米腔的集成生物感測器及其製作方法 |
| US10174371B2 (en) | 2015-08-05 | 2019-01-08 | Genia Technologies, Inc. | Use of titanium nitride as an electrode in non-faradaic electrochemical cell |
| US10809243B2 (en) | 2015-08-31 | 2020-10-20 | Roche Sequencing Solutions, Inc. | Small aperture large electrode cell |
| WO2017050723A1 (en) | 2015-09-22 | 2017-03-30 | Genia Technologies, Inc. | Pol7 polymerase variants |
| US10102338B2 (en) * | 2015-09-24 | 2018-10-16 | Genia Technologies, Inc. | Adaptive compression and modification of nanopore measurement data |
| EP3353317A1 (en) | 2015-09-24 | 2018-08-01 | H. Hoffnabb-La Roche Ag | Alpha-hemolysin variants |
| US10935512B2 (en) | 2015-09-24 | 2021-03-02 | Roche Sequencing Solutions, Inc. | Encoding state change of nanopore to reduce data size |
| US20180299400A1 (en) * | 2015-10-21 | 2018-10-18 | Genia Technologies, Inc. | Use of fluoropolymers as a hydrophobic layer to support lipid bilayer formation for nanopore based dna sequencing |
| CN105368938B (zh) * | 2015-11-06 | 2018-09-21 | 中国科学院重庆绿色智能技术研究院 | 一种基于电击穿在氮化硅薄膜上精确制备纳米孔的方法 |
| US10718737B2 (en) | 2016-01-21 | 2020-07-21 | Roche Sequencing Solutions, Inc. | Molded flow channel |
| JP6959931B2 (ja) | 2016-02-29 | 2021-11-05 | ジェニア・テクノロジーズ・インコーポレイテッド | エキソヌクレアーゼ欠損ポリメラーゼ |
| US10465240B2 (en) * | 2016-03-30 | 2019-11-05 | Roche Sequencing Solutions, Inc. | Electrical enhancement of bilayer formation |
| EP3436602B1 (en) | 2016-03-31 | 2024-08-14 | Genia Technologies, Inc. | Nanopore protein conjugates and uses thereof |
| EP3445775A1 (en) | 2016-04-21 | 2019-02-27 | H. Hoffnabb-La Roche Ag | Alpha-hemolysin variants and uses thereof |
| JP6695450B2 (ja) | 2016-06-27 | 2020-05-20 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 二重層形成のための浸透性不均衡法 |
| CN109415419B (zh) | 2016-06-30 | 2022-07-29 | 豪夫迈·罗氏有限公司 | 长寿α-溶血素纳米孔 |
| GB201612458D0 (en) * | 2016-07-14 | 2016-08-31 | Howorka Stefan And Pugh Genevieve | Membrane spanning DNA nanopores for molecular transport |
| CN106443008A (zh) * | 2016-08-31 | 2017-02-22 | 中国科学院重庆绿色智能技术研究院 | 一种基于固态纳米孔的hiv‑1蛋白酶检测方法 |
| CN110114458A (zh) | 2016-09-22 | 2019-08-09 | 豪夫迈·罗氏有限公司 | Pol6聚合酶变体 |
| CA3050695C (en) | 2017-01-20 | 2024-02-20 | Omniome, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
| CN110366680B (zh) * | 2017-02-14 | 2021-10-08 | 阿克斯比尔公司 | 用于大分子的连续诊断的设备和方法 |
| EP3655423B1 (en) * | 2017-07-17 | 2023-12-20 | President and Fellows of Harvard College | Nanopore-matched protein shuttle for molecular characterization |
| EP3697932A1 (en) * | 2017-10-19 | 2020-08-26 | Omniome, Inc. | Simultaneous background reduction and complex stabilization in binding assay workflows |
| WO2019081442A1 (en) * | 2017-10-23 | 2019-05-02 | F. Hoffmann-La Roche Ag | REMOVAL AND REINTEGRATION OF PROTEIN NANOPORES IN A MEMBRANE USING AN OSMOTIC IMBALANCE |
| CN109709185B (zh) * | 2017-10-25 | 2024-09-24 | 深圳宣泽生物医药有限公司 | 一种修饰生物探针的纳米孔检测装置及制作方法 |
| EP3752522B1 (en) | 2018-02-15 | 2024-03-27 | F. Hoffmann-La Roche AG | Nanopore protein conjugates for detection and analysis of analytes |
| WO2019166458A1 (en) | 2018-02-28 | 2019-09-06 | F. Hoffmann-La Roche Ag | Alpha-hemolysin variants and uses thereof |
| WO2019197590A1 (en) | 2018-04-13 | 2019-10-17 | F. Hoffmann-La Roche Ag | Methods and compositions for detection and analysis of analytes |
| US20210381041A1 (en) | 2018-05-28 | 2021-12-09 | Roche Sequencing Solutions, Inc. | Enzymatic Enrichment of DNA-Pore-Polymerase Complexes |
| EP3815092A2 (en) | 2018-06-29 | 2021-05-05 | F. Hoffmann-La Roche AG | Detection of microsatellite instability |
| CN113260449B (zh) * | 2018-12-11 | 2023-09-29 | 豪夫迈·罗氏有限公司 | 用于膜中自限性蛋白质孔插入的系统和方法 |
| JP7162283B2 (ja) * | 2019-05-27 | 2022-10-28 | 国立大学法人山形大学 | 脂質二分子膜チャネル評価チップ及びその製造方法並びに評価装置 |
| WO2021013805A1 (en) | 2019-07-22 | 2021-01-28 | F. Hoffmann-La Roche Ag | Systems and methods for cell of origin determination from variant calling data |
| WO2021021944A1 (en) * | 2019-07-31 | 2021-02-04 | Axbio Inc. | Systems and methods for assessing a target molecule |
| US20220372566A1 (en) | 2019-09-20 | 2022-11-24 | Roche Sequencing Solutions, Inc. | Immune repertoire profiling by primer extension target enrichment |
| WO2021087118A1 (en) * | 2019-10-30 | 2021-05-06 | Stratos Genomics, Inc. | Methods and compositions for assembly of biological nanopores |
| US20220392572A1 (en) | 2019-11-21 | 2022-12-08 | Roche Sequencing Solutions, Inc. | Systems and methods for contamination detection in next generation sequencing samples |
| JP7767320B2 (ja) | 2020-05-28 | 2025-11-11 | エフ． ホフマン－ラ ロシュ アーゲー | 高エラー単一分子読み取りにおいて短いモチーフを同定するための配列位置合わせシステムおよび方法 |
| CA3191929A1 (en) * | 2020-09-08 | 2022-03-17 | Harold G. Monbouquette | Lateral flow nucleic acid assay with integrated pore-based detection |
| CN114249294B (zh) * | 2020-09-22 | 2025-07-04 | 上海新微技术研发中心有限公司 | 一种多层mems结构及其制作方法与应用 |
| US12378596B2 (en) | 2020-12-03 | 2025-08-05 | Roche Sequencing Solutions, Inc. | Whole transcriptome analysis in single cells |
| JP7646850B2 (ja) | 2021-02-09 | 2025-03-17 | エフ． ホフマン－ラ ロシュ アーゲー | 核酸中のメチル化の塩基レベル検出のための方法 |
| US20240301394A1 (en) | 2021-03-03 | 2024-09-12 | Roche Sequencing Solutions, Inc. | Devices and methods for electrophoretic extraction of nucleic acids from biological samples |
| CN115125098B (zh) * | 2021-03-29 | 2025-05-27 | 上海近观科技有限责任公司 | 基于分子封层及加热密封结构的纳米孔检测装置及方法 |
| US20240240240A1 (en) | 2021-05-24 | 2024-07-18 | Roche Sequencing Solutions, Inc | Enhancer oligonucleotides for nucleic acid hybridization |
| MX2024006859A (es) | 2021-12-07 | 2024-06-19 | Caribou Biosciences Inc | Un metodo de captura de productos de escision de endonucleasas crispr. |
| CN114410459A (zh) * | 2022-01-11 | 2022-04-29 | 深圳清华大学研究院 | 基因测序装置和测序方法 |
| US20250361549A1 (en) | 2022-06-14 | 2025-11-27 | Roche Sequencing Solutions, Inc. | Detection of epigenetic cytosine modification |
| EP4573207A1 (en) | 2022-08-18 | 2025-06-25 | F. Hoffmann-La Roche AG | Detection of epigenetic modifications |
| TWI862997B (zh) * | 2022-09-30 | 2024-11-21 | 國立中興大學 | 微流體感測裝置、微流體感測系統及微流體感測方法 |
| CN115651821B (zh) * | 2022-12-07 | 2023-04-07 | 北京齐碳科技有限公司 | 一种分子检测单元、芯片以及制备方法 |
| CN116381250A (zh) * | 2023-03-03 | 2023-07-04 | 南京大学 | 基于纳米孔道蛋白的高通量检测装置 |
| AU2024246785A1 (en) | 2023-03-30 | 2025-09-11 | F. Hoffmann-La Roche Ag | Modulation of target molecule-lipid bilayer interactions |
| WO2025024676A1 (en) | 2023-07-26 | 2025-01-30 | Caribou Biosciences, Inc. | In vitro validation methods for cd19-targeting cell therapies |
| US20250161882A1 (en) * | 2023-11-17 | 2025-05-22 | Illumina, Inc. | Fluidic devices, nanopore instruments, and methods |
| WO2025193609A1 (en) | 2024-03-11 | 2025-09-18 | Caribou Biosciences, Inc. | Methods and compositions for cost-effective assessment of nucleic acid transcripts and isoforms in cells |
| WO2025221998A1 (en) | 2024-04-17 | 2025-10-23 | Roche Sequencing Solutions, Inc. | Systems and methods for variant calling |
| WO2025221988A1 (en) | 2024-04-17 | 2025-10-23 | Roche Sequencing Solutions, Inc. | Systems and methods for somatic small variant calling |
| WO2025231432A1 (en) | 2024-05-03 | 2025-11-06 | Caribou Biosciences, Inc. | In vivo gene editing with crispr systems |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101932933A (zh) * | 2007-12-19 | 2010-12-29 | 牛津纳米孔技术有限公司 | 两性分子层的形成 |
| US20110193249A1 (en) * | 2010-02-08 | 2011-08-11 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| WO2011097028A1 (en) * | 2010-02-08 | 2011-08-11 | Genia Technologies, Inc. | Systems and methods for manipulating a molecule in a nanopore |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0637384B1 (en) * | 1992-04-22 | 1996-10-02 | Ecole Polytechnique Federale De Lausanne | Lipid membrane sensors |
| US6413792B1 (en) * | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US6952651B2 (en) * | 2002-06-17 | 2005-10-04 | Intel Corporation | Methods and apparatus for nucleic acid sequencing by signal stretching and data integration |
| JP2006526777A (ja) * | 2003-02-28 | 2006-11-24 | ブラウン ユニバーシティー | ナノ細孔、その使用方法、その作成方法、及びそれを使用して生体分子を特徴付ける方法 |
| US20050023156A1 (en) * | 2003-07-30 | 2005-02-03 | Ramsey J. Michael | Nanostructured material transport devices and their fabrication by application of molecular coatings to nanoscale channels |
| US20050136408A1 (en) * | 2003-12-19 | 2005-06-23 | May Tom-Moy | Methods and systems for characterizing a polymer |
| JP4953044B2 (ja) | 2005-05-09 | 2012-06-13 | 財団法人生産技術研究奨励会 | 脂質二重膜の形成方法およびその装置 |
| AU2006249340A1 (en) * | 2005-05-26 | 2006-11-30 | Ensemble Discovery Corportion | Biodetection by nucleic acid-templated chemistry |
| US8038885B2 (en) | 2005-10-14 | 2011-10-18 | The Regents Of The University Of California | Formation and encapsulation of molecular bilayer and monolayer membranes |
| US7777505B2 (en) * | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
| NZ579083A (en) | 2007-02-20 | 2012-07-27 | Oxford Nanopore Tech Ltd | Lipid bilayer sensor system |
| AU2008236694B2 (en) * | 2007-04-04 | 2014-01-23 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| GB0713402D0 (en) | 2007-07-11 | 2007-08-22 | Cardiff & Vale Nhs Trust | A method of diagnosing a condition using a neural network |
| BRPI0813718A2 (pt) * | 2007-07-13 | 2014-12-30 | Univ Leland Stanford Junior | Método e aparelho que utilizam um campo elétrico para ensaios biológicos aperfeiçoados |
| JP5441142B2 (ja) * | 2007-11-26 | 2014-03-12 | 国立大学法人 東京大学 | マイクロ流体による平面脂質二重膜アレイ及びその平面脂質二重膜を用いた分析方法 |
| WO2009073622A1 (en) * | 2007-11-30 | 2009-06-11 | Electronic Bio Sciences, Llc | Method and apparatus for single side bilayer formation |
| WO2009117522A2 (en) * | 2008-03-18 | 2009-09-24 | Reinhart, Kevin | Nanopore and carbon nanotube based dna sequencer and a serial recognition sequencer |
| WO2010082860A1 (en) * | 2009-01-19 | 2010-07-22 | Instituto De Biologia Experimental E Tecnologia (Ibet) | Method and device for nanopore based single-molecule protein/ protein interaction detection |
| JP5372570B2 (ja) * | 2009-03-30 | 2013-12-18 | 株式会社日立ハイテクノロジーズ | ナノポアを用いたバイオポリマー決定方法、システム、及びキット |
| JP2013506418A (ja) * | 2009-09-30 | 2013-02-28 | クアンタポール， インコーポレイテッド | 標識されたナノポアを使用する生物学的なポリマーの超高速配列決定 |
| US8324914B2 (en) | 2010-02-08 | 2012-12-04 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
| JP6016792B2 (ja) * | 2010-07-14 | 2016-10-26 | ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ | ナノポア促進型の核酸の単分子検出 |
| GB2500360B (en) | 2010-12-22 | 2019-10-23 | Genia Tech Inc | Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps |
| US8968539B2 (en) * | 2011-03-08 | 2015-03-03 | Electronic Biosciences, Inc. | Methods for voltage-induced protein incorporation into planar lipid bilayers |
| JP6298404B2 (ja) * | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| EP2814983B1 (en) | 2012-02-16 | 2019-04-24 | Genia Technologies, Inc. | Methods for creating bilayers for use with nanopore sensors |
| JP2020504808A (ja) * | 2016-10-26 | 2020-02-13 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ナノポア配列決定用の集積回路およびフローセルのマルチチップパッケージング |
-
2013
- 2013-02-15 EP EP13748712.0A patent/EP2814983B1/en active Active
- 2013-02-15 US US14/376,836 patent/US9850534B2/en active Active
- 2013-02-15 ES ES13748712T patent/ES2732256T3/es active Active
- 2013-02-15 WO PCT/US2013/026514 patent/WO2013123450A1/en not_active Ceased
- 2013-02-15 CN CN201810865708.1A patent/CN108949944A/zh active Pending
- 2013-02-15 CN CN201380015148.6A patent/CN104254619B/zh active Active
- 2013-02-15 CA CA2864125A patent/CA2864125C/en active Active
- 2013-02-15 JP JP2014557852A patent/JP6178805B2/ja active Active
- 2013-02-15 EP EP19165626.3A patent/EP3540077B1/en active Active
-
2017
- 2017-03-15 JP JP2017049533A patent/JP6431108B2/ja active Active
- 2017-11-10 US US15/809,725 patent/US10316360B2/en active Active
-
2018
- 2018-02-28 JP JP2018034689A patent/JP6617167B2/ja active Active
-
2019
- 2019-04-26 US US16/396,284 patent/US11299781B2/en active Active
- 2019-05-20 JP JP2019094447A patent/JP2019164155A/ja not_active Withdrawn
-
2022
- 2022-03-04 US US17/687,003 patent/US12077817B2/en active Active
-
2024
- 2024-07-25 US US18/784,427 patent/US20250027148A1/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101932933A (zh) * | 2007-12-19 | 2010-12-29 | 牛津纳米孔技术有限公司 | 两性分子层的形成 |
| US20110193249A1 (en) * | 2010-02-08 | 2011-08-11 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| WO2011097028A1 (en) * | 2010-02-08 | 2011-08-11 | Genia Technologies, Inc. | Systems and methods for manipulating a molecule in a nanopore |
Non-Patent Citations (2)
| Title |
|---|
| 冯辉等: "基于电聚合硫堇膜与纳米复合材料界面的直接电化学免疫传感器的构建 ", 《传感技术学报》 * |
| 董杉木等: "纳米多孔硅阻抗生物传感器的研究 ", 《生物加工过程》 * |
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