CN108901853B - Method for disinfecting canker germs in kiwi fruit branches and nursery stock tissues - Google Patents
Method for disinfecting canker germs in kiwi fruit branches and nursery stock tissues Download PDFInfo
- Publication number
- CN108901853B CN108901853B CN201810988369.6A CN201810988369A CN108901853B CN 108901853 B CN108901853 B CN 108901853B CN 201810988369 A CN201810988369 A CN 201810988369A CN 108901853 B CN108901853 B CN 108901853B
- Authority
- CN
- China
- Prior art keywords
- kiwi fruit
- branches
- seedlings
- nursery stock
- tissues
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000298697 Actinidia deliciosa Species 0.000 title claims abstract description 70
- 235000009436 Actinidia deliciosa Nutrition 0.000 title claims abstract description 70
- 244000052616 bacterial pathogen Species 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000000249 desinfective effect Effects 0.000 title claims description 9
- 239000004033 plastic Substances 0.000 claims abstract description 18
- 229920003023 plastic Polymers 0.000 claims abstract description 18
- 230000001954 sterilising effect Effects 0.000 claims abstract description 16
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 244000052769 pathogen Species 0.000 claims description 6
- 235000009434 Actinidia chinensis Nutrition 0.000 claims description 5
- 208000025865 Ulcer Diseases 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 4
- 231100000397 ulcer Toxicity 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims 3
- -1 polyethylene Polymers 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 33
- 241000196324 Embryophyta Species 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 230000002265 prevention Effects 0.000 description 5
- 239000013043 chemical agent Substances 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 238000010008 shearing Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000009084 Cold Injury Diseases 0.000 description 1
- JJLJMEJHUUYSSY-UHFFFAOYSA-L Copper hydroxide Chemical compound [OH-].[OH-].[Cu+2] JJLJMEJHUUYSSY-UHFFFAOYSA-L 0.000 description 1
- 239000005750 Copper hydroxide Substances 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000002595 cold damage Effects 0.000 description 1
- 229910001956 copper hydroxide Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- PVTHJAPFENJVNC-MHRBZPPQSA-N kasugamycin Chemical compound N[C@H]1C[C@H](NC(=N)C(O)=O)[C@@H](C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)[C@@H]1O PVTHJAPFENJVNC-MHRBZPPQSA-N 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a sterilization method for canker germs in kiwi fruit branches and nursery stock tissues, which is mainly characterized in that the kiwi fruit branches or seedlings are put into a watertight PE plastic bag, clear water is injected into the PE plastic bag, the PE plastic bag is placed in an ultrasonic field environment at the temperature of 45-50 ℃ for ultrasonic treatment for 30-60min, the ultrasonic frequency is 60-100Hz, then the branches or the nursery stock are taken out and dried, and the sterilization of the canker germs in the kiwi fruit branches and the nursery stock tissues is completed.
Description
Technical Field
The invention relates to a method for sterilizing canker germs in kiwi fruit branches and nursery stock tissues through heat and ultrasonic treatment, belongs to a comprehensive control technology of kiwi fruit canker, and is particularly suitable for comprehensive control of canker in a new development area of kiwi fruit.
Background
The bacterial canker of the kiwi fruit is a disease which can cause the devastating of the kiwi fruit industry, the canker of the kiwi fruit growing areas of the world, New Zealand, Spanish, Italy, Turkey and the kiwi fruit growing areas of 16 provinces of Shaanxi, Sichuan and Anhui of the kiwi fruit growing areas of China all occur, and the development of the world and the kiwi fruit industry of China is seriously threatened. The kiwifruit canker has been treated as a plant quarantine object and a in china, new zealand, the united states and Europe Plant Protection Organization (EPPO), respectively2Quarantine pests to control the rapid spread of the disease. The Chinese gooseberry canker pathogenic bacteria are clove Pseudomonas syringae pathogenic variants (Psa) which can invade into a plant body through stomata, skin pores, wounds (insect injury, cold injury, knife injury) and the like of the plant and damage the trunk, branches, tender tips, leaves, flowers and other parts; in the field susceptible specimens, Psa was observed to colonize mainly in cortex, phloem and xylem vessels in shoot tissues and in the upper and lower epidermis and mesophyll cell gaps in leaf tissues.
At present, the prevention and treatment of kiwifruit canker in production mainly adopts a means of combining agricultural prevention and treatment, chemical prevention and treatment and biological prevention and treatment. The biological control is also in the testing stage of screening of beneficial microorganisms and field control; the main chemical agents of the chemical prevention and treatment commonly used medicaments are pesticides such as copper hydroxide and kasugamycin, on one hand, as the Psa is fixedly planted in phloem and xylem ducts of branches, the chemical agents only can kill the Psa shown by the branches when treating the branches and seedlings, and have no killing effect on the Psa in the tissues of the branches and the seedlings, the currently used chemical pesticides can only reduce the incidence of kiwifruit canker to a certain extent, but can not radically cure the canker, and trees and gardens with serious incidence can not be used as special-effect medicaments and can only dig up and damage the trees; on the other hand, the long-term use of a large amount of chemical pesticides can cause the plants to generate drug resistance.
Aiming at the outstanding problems that Psa fixedly planted in tissues such as cortex, phloem and xylem vessels of branch tissues is difficult to kill by chemical agents and is difficult to prevent and treat after the disease of the ulcer disease is developed, the invention adopts ultrasonic and heat treatment to treat the kiwi fruit branches and seedlings, kills the Psa of the branches and seedling tissues and effectively prevents the spread of the ulcer disease pathogen of the kiwi fruit seedlings and scions.
Disclosure of Invention
In order to overcome the defects of the existing kiwi fruit branch and seedling disinfection technology, the invention provides a method for disinfecting canker germs in kiwi fruit branch and seedling tissues, which can kill Psa of the branch and seedling tissues and effectively prevent canker germs from being spread by kiwi fruit seedlings and scions.
The technical scheme adopted by the invention is as follows:
a disinfection method for canker germs in kiwi fruit branches and nursery stock tissues comprises the following specific steps:
cutting kiwi fruit branches or seedlings to a proper length, filling the kiwi fruit branches or seedlings into a PE plastic bag, injecting clear water into the PE plastic bag until the branches or seedlings are submerged, placing the PE plastic bag in an ultrasonic field environment at the temperature of 45-50 ℃ for ultrasonic treatment for 30-60min at the ultrasonic frequency of 60-100Hz, taking out the branches or seedlings, and drying the branches or seedlings in the air to sterilize ulcer germs in the kiwi fruit branches and seedling tissues.
Further limiting, selecting 2-year or current-year branches as the kiwi branches, shearing the branches to be about 5cm in length, and disinfecting the branches.
Further limiting, the kiwi fruit seedlings are seedlings grown for 1 year or two years, young shoots of the overground parts of the kiwi fruit seedlings are cut off about 10cm above the junction of the young shoots and the young shoots are cut off, and the seedlings after the young shoots are disinfected.
Further limiting, the kiwi fruit branches and the nursery stock tissues are cortex tissues, phloem tissues and xylem vessel tissues of the kiwi fruit branches and the nursery stock, not only can have a sterilization effect on germs outside the epidermis of the branches or the nursery stock, but also more importantly can have a sterilization effect on germs of internal tissues such as cortex tissues, phloem tissues and xylem vessel tissues, and the like, and the tissues are not damaged, and the normal development of the tissues is not influenced.
Further limiting, the ultrasonic treatment condition is that the ultrasonic treatment is carried out for 40-50 min in an ultrasonic field environment at the temperature of 45 ℃, the ultrasonic frequency is 70-80 Hz, and the temperature and the ultrasonic supplement each other to achieve synergistic effect.
The method for disinfecting the canker germs in the kiwi fruit branches and the nursery stock tissues mainly aims at solving the outstanding problems that the Psa fixedly planted in the tissues such as the cortex, phloem and xylem vessels of the branch tissues is difficult to kill by chemical agents and the canker germs are difficult to prevent and treat after the onset of the canker germs, adopts the ultrasonic and heat treatment to treat the kiwi fruit branches and the nursery stock tissues to kill the Psa of the branches and the nursery stock tissues, effectively prevents the canker germs from being transmitted by the kiwi fruit nursery stock and scion, particularly has the bactericidal rate of 97.75 percent on the canker germs in the two-year or one-year branch tissues, but has no influence on the normal growth of the nursery stock.
Drawings
Fig. 1 is a graph showing the effect of sterilization rate on the treatment group 1 at different ultrasonic frequencies.
Detailed Description
The technical solution of the present invention will be further explained with reference to the drawings and examples, but the present invention is not limited to the following implementation cases.
Example 1
The method for sterilizing ulcer germs by selecting kiwi branches as objects comprises the following steps:
(1) cutting 2-year or current-year branches of kiwi fruits into about 5 cm;
(2) putting into a watertight PE plastic bag, and injecting clear water into the PE plastic bag until the branches are submerged;
(3) placing the PE plastic bag filled with branches and clear water into an ultrasonic processor, heating to 45 ℃, performing ultrasonic treatment for 45min under the condition that the ultrasonic frequency is 80Hz, and treating the kiwi branch tissue;
(4) and taking out the branches immediately after the treatment is finished, airing the branches to sterilize canker germs in kiwi fruit branch tissues, and storing the aired branches conventionally or directly using the aired branches for grafting.
Example 2
The method selects kiwi fruit seedlings as treatment objects to disinfect canker germs, and comprises the following steps:
(1) completely digging out kiwi fruit seedlings growing for 1 year or two years from a kiwi fruit nursery, and shearing off young shoots of overground parts from positions about 10cm above a root-stem junction;
(2) putting the nursery stock with the cut young shoots and roots into a watertight PE plastic bag, and then injecting clear water into the PE plastic bag until the whole nursery stock is submerged;
(3) putting the PE plastic bag filled with the nursery stock and clear water into an ultrasonic processor, heating to 50 ℃, and carrying out ultrasonic treatment for 30min under the condition that the ultrasonic frequency is 100Hz to treat the kiwi fruit branch tissue;
(4) and immediately taking out the nursery stock from the PE belt after the treatment, and airing the nursery stock for temporary planting or direct planting and nursery stock sale.
Example 3
The method selects kiwi fruit seedlings as treatment objects to disinfect canker germs, and comprises the following steps:
(1) completely digging out kiwi fruit seedlings growing for 1 year or two years from a kiwi fruit nursery, and shearing off new shoots of overground parts from positions 10cm above a root-stem junction;
(2) putting the nursery stock with the cut young shoots and roots into a watertight PE plastic bag, and then injecting clear water into the PE plastic bag until the whole nursery stock is submerged;
(3) putting the PE plastic bag filled with the nursery stock and clear water into an ultrasonic processor, heating to 45 ℃, and carrying out ultrasonic treatment for 50min under the condition that the ultrasonic frequency is 70Hz to treat the kiwi fruit branch tissue;
(4) and immediately taking out the nursery stock from the PE belt after the treatment, and airing the nursery stock for temporary planting or direct planting and nursery stock sale.
In the embodiment, the kiwi fruit branches or seedlings are heated to 45-50 ℃ firstly, and ultrasonic treatment at 60-100Hz is combined for 30-60min, so that Psa of the branches and seedling tissues can be effectively killed, the kiwi fruit seedlings and scions are prevented from spreading canker pathogen, and the branches or seedlings can be ensured to grow healthily.
In order to verify the sterilization effect of the sterilization agent on the canker pathogenic bacteria of the kiwi fruit branches or seedlings, the following experiments are combined for explanation.
The disease-sensitive branches of kiwifruit canker: performing field investigation in Bairui kiwi fruit Innovative gardens from weeks to counties in Shaanxi province, cutting branches presenting red pyogenic bacteria with typical symptoms of canker, packaging in a sterilized self-sealing bag, and storing at 4 ℃ for later use.
1-year-old kiwi fruit seedlings: purchased from Bairui Kiwi fruit, Xinyuan, Zhou of Shaanxi province, county.
And (3) sterilizing the surfaces of the branches by selecting 0.6% NaClO for 10min, and then cleaning the surfaces of the branches by using sterilized distilled water for 2-3 times to ensure that the NaClO disinfectant on the surfaces of the branches is fully washed away.
Treatment group 1: selecting the frequency of 0, 20, 40, 60, 80 and 100Hz, treating the branches at 45 ℃, performing ultrasonic treatment for 30min without any treatment on the control group 1, counting the growth conditions of the control group 1 and the treated group Psa by a colony counting method, and calculating the sterilization rate by the following formula: (the number of colonies picked was between 30 and 300).
The growth of Psa was counted by colony counting between control 1 and treatment groups, and the results are shown in fig. 1.
As can be seen from figure 1, the bacteriostatic rate of the branches can only reach 48% when the branches are independently heated to 45 ℃ for treatment; after combined ultrasonic treatment, along with the continuous increase of ultrasonic frequency, the sterilization rate is continuously increased, and when the frequency reaches 80Hz, the sterilization rate reaches 97.11 percent; when the ultrasonic frequency reaches 100Hz, the sterilization rate is basically consistent with that of 80 Hz.
Treatment group 2: treating 30 seedlings at 45 deg.C with ultrasonic frequency of 80Hz for 45min, and repeating for three times; control 2 was a normal healthy seedling without any treatment. The nursery stocks of the treatment group and the control group 2 are planted in a Bairui kiwi fruit creative garden from Zhou to county in Shaanxi province, the survival rate of the nursery stocks is investigated in 2018, 4 and 1, the growth condition of the nursery stocks is investigated in 2018.7.25, the total number of leaves of each nursery stock is counted, the diameter of a nursery stock foundation, the height of the nursery stock plant and the length and width of the leaves are measured by a vernier caliper, and the measurement results of relevant indexes are shown in the following table 1:
table 1 shows the results of the measurement of the seedling quality indexes of the treated group 2 and the control group 2
| Test grouping | Survival rate | Diameter of blade | Height of seedling | Number of blades | Diameter of the earth |
| Treatment group 2 | 100％ | 8.13±0.98 | 52.13±2.53 | 23.33±1.53 | 1.32±0.05 |
| Control group 2 | 100％ | 7.85±1.76 | 49.74±1.69 | 21.67±1.99 | 1.27±0.11 |
As can be seen from Table 1, the method for sterilizing the nursery stocks has the advantages that the nursery stocks in the treated group do not show morbidity, the treated group is completely survived, and the leaf diameter, the seedling height, the leaf number and the ground diameter of the nursery stocks are all compared with those in the control group 2 from the aspect of the growth of the nursery stocks, so that the method has no significant difference.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present invention as described in the specification, or directly or indirectly applied to other related fields, are included in the scope of the present invention.
Claims (5)
1. A sterilization method for canker germs in kiwi fruit branches and nursery stock tissues is characterized by comprising the following steps:
cutting kiwi fruit branches or seedlings to a proper length, filling the kiwi fruit branches or seedlings into a watertight PE plastic bag, injecting clear water into the PE plastic bag until the branches or seedlings are submerged, placing the polyethylene plastic bag in an ultrasonic field environment at the temperature of 45-50 ℃ for ultrasonic treatment for 30-60min at the ultrasonic frequency of 60-100Hz, taking out the branches or seedlings, and airing to sterilize ulcer germs in the kiwi fruit branches and seedling tissues.
2. The method for disinfecting canker pathogens in kiwi fruit branches and nursery stock tissues according to claim 1, which is characterized in that: the kiwi branches are 2-year-old or current-year-old branches, and the length of each kiwi branch is cut to be 5 cm.
3. The method for disinfecting canker pathogens in kiwi fruit branches and nursery stock tissues according to claim 1, which is characterized in that: the kiwi fruit seedlings are 1-year or two-year old seedlings, young shoots of the overground parts of the kiwi fruit seedlings are cut off 10cm above the junction of the kiwi fruit seedlings and the roots, and the seedlings with the young shoots cut off are disinfected.
4. The method for disinfecting canker pathogens in kiwi fruit branches and nursery stock tissues according to claim 1, which is characterized in that: the kiwi fruit branches and the nursery stock tissues are the cortex tissues, phloem and xylem vessel tissues of the kiwi fruit branches and the nursery stock.
5. The method for disinfecting canker pathogens in kiwi fruit branches and nursery stock tissues according to claim 1, which is characterized in that: the ultrasonic treatment condition is that the ultrasonic treatment is carried out for 40-50 min in an ultrasonic field environment at the temperature of 45 ℃, and the ultrasonic frequency is 70-80 Hz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810988369.6A CN108901853B (en) | 2018-08-28 | 2018-08-28 | Method for disinfecting canker germs in kiwi fruit branches and nursery stock tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810988369.6A CN108901853B (en) | 2018-08-28 | 2018-08-28 | Method for disinfecting canker germs in kiwi fruit branches and nursery stock tissues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108901853A CN108901853A (en) | 2018-11-30 |
| CN108901853B true CN108901853B (en) | 2020-06-16 |
Family
ID=64406567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810988369.6A Active CN108901853B (en) | 2018-08-28 | 2018-08-28 | Method for disinfecting canker germs in kiwi fruit branches and nursery stock tissues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108901853B (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018050483A (en) * | 2016-09-26 | 2018-04-05 | 株式会社東芝 | Processing method for dry process |
| CN107371463A (en) * | 2017-09-19 | 2017-11-24 | 孙立成 | A kind of pepper seed poison-removing method |
-
2018
- 2018-08-28 CN CN201810988369.6A patent/CN108901853B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN108901853A (en) | 2018-11-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104604609B (en) | A kind of method for preventing and kill off Prospect on Kiwifruit Bacterial Canker | |
| CN103992189A (en) | Compound Chinese herbal botanical insecticide-fungicide and preparation method thereof | |
| CN105379589A (en) | Method for preventing and treating kiwifruit canker | |
| CN107810950B (en) | Method for preventing and treating aniseed anthracnose | |
| CN106471931A (en) | A kind of implantation methods of organic hot pepper | |
| CN108375658B (en) | Method for evaluating control effect of bactericide on black spot of potato seedlings | |
| CN104770157A (en) | Cuttage breeding method for increasing survival rate of speed grows willows | |
| CN103798036B (en) | The cultivation method of a kind of strawberry | |
| CN105210600A (en) | Virus-free basic potato seed hot-house culture method | |
| CN103798068B (en) | A kind of processing method of strawberry seedling | |
| CN103797930B (en) | A kind of strawberry seedling with excellent insect-pest performance | |
| CN108901853B (en) | Method for disinfecting canker germs in kiwi fruit branches and nursery stock tissues | |
| Padhi et al. | Surface sterilization for reducing microbial contamination in in vitro propagation of lasora (Cordia myxa Roxb.) using nodal segments | |
| CN109169105A (en) | A kind of normalized planting method of bletilla striata good quality and high output | |
| CN112930984A (en) | Method for preventing and treating pear apple branch blight | |
| Opara et al. | Control of Bacterial Spot Diseases of Solanum | |
| CN106031349B (en) | Method for evaluating eradication effect of bactericide on rot germs on surface layers of branches | |
| CN104335845A (en) | Method for prevention and treatment of tomato late blight | |
| CN110771410B (en) | Cultivation method capable of improving disease resistance of vegetables | |
| CN111990143A (en) | Agricultural method for preventing fruit tree infectious diseases | |
| CN114041383B (en) | Ecological planting technology | |
| CN103053427B (en) | Stem tip sterilizing primary culture method for germination accelerating for horseradish seeds | |
| CN109566243A (en) | A method of prevention and treatment oil tea pest angle chest is chrysomelid | |
| CN106962115A (en) | A kind of method for improving willow resistance against diseases | |
| CN109042685B (en) | Composite antibacterial agent and use method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20241029 Address after: No. 045, Xujiayuan Village, Dihua Town, Danfeng County, Shangluo City, Shaanxi Province, China 726000 Patentee after: Shangluo Guoyun Agricultural and Forestry Technology Co.,Ltd. Country or region after: China Address before: No. 199, Chang'an South Road, changyanbao office, Yanta District, Xi'an City, Shaanxi Province Patentee before: Shaanxi Normal University Country or region before: China |