CN109042685B

CN109042685B - Composite antibacterial agent and use method thereof

Info

Publication number: CN109042685B
Application number: CN201810962206.0A
Authority: CN
Inventors: 李欢; 陈凤毛; 李岱龙; 赵祥慧; 王文文; 程娟
Original assignee: Nanjing Forestry University
Current assignee: Nanjing Forestry University
Priority date: 2018-08-22
Filing date: 2018-08-22
Publication date: 2020-06-30
Anticipated expiration: 2038-08-22
Also published as: CN109042685A

Abstract

The invention discloses a composite antibacterial agent and a using method thereof, wherein the composite antibacterial agent comprises a solid medicament and a liquid medicament; wherein the solid medicament comprises 0.1-1g/kg of polyacrylamide, and the balance of nutrient soil; wherein the liquid medicament comprises 0.5-1.5ml/kg 1% shenqinmycin; 0.25-1.5ml/kg of organosilicon, and the balance of sterile water. Aiming at the acer truncatum wilt caused by a soil-borne pathogen Verticillium dahliae, the composite antibacterial agent disclosed by the invention utilizes a biological source medicament of shenqinmycin, combines organic silicon and polyacrylamide to carry out composite proportioning, has an internal absorption effect far superior to that of other medicaments, can kill pathogens colonized at roots and vascular tissues, and effectively controls the disease state; the water retention and synergistic components can reduce water and medicament loss, reduce application times and labor input, and are particularly suitable for water-deficient and drought areas.

Description

Composite antibacterial agent and use method thereof

Technical Field

The invention relates to the technical field of agricultural bactericide preparations, in particular to a composite antibacterial agent and a using method thereof.

Background

The pathogen of the acer truncatum wilt Disease is screened and identified as Verticillium dahliae (Verticillium dahliae) by a chenfeng hair problem group of Nanjing forestry university, related results are published in Plant Disease of International Plant pathology journal, a control method of the acer truncatum dahliae is not overcome, and the technical research is developed on the premise. The characteristics and the occurrence rule of the disease, the pathogen infection object, the disease circulation process and the known prevention and control method are briefly described as follows.

Disease characteristics and occurrence rules: the acer truncatum wilt disease occurs in low-lying farmland which is easy to accumulate water, and is interplanted with crops; initially, diseased trees are distributed randomly, and the number of diseased trees in individual plots is large (southwest and northeast plots), and then the diseased trees spread rapidly around the diseased trees as the center. The back warming occurs at the end of four months, and the outbreak stops along with the rise of the temperature in five and six months; in September, the air is cooled again until the leaves fall in November. When the disease occurs, the leaves are withered and dehydrated, then the whole plant is withered in a short period, the leaves do not fall off and lose green, and the leaves of the dead plant are turned into withered yellow after a period of time and do not fall off; the phloem of the dissected phloem of all diseased trees can be seen to be gray black, the roots can also be dehydrated, dried and blackened, and the vascular tissues on the cross sections of the trunks of dead plants are woven to form obvious brown or dark green annular color-changing areas; one side of a part of diseased trees is withered, and the other parts are normal; occasionally, the plant withers in the current year, and withers again after the root of the trunk sprouts again in the next year, so that the diseased plant is difficult to treat and recover.

Pathogens may invade from the root through a skin hole or a wound caused by agricultural operation, and further invade xylem vascular tissues to colonize and grow in large quantities, so that a catheter is blocked and water transportation is blocked; the plants can not transport water upwards for the transpiration and photosynthesis of leaves, the normal physiological metabolism of the leaves is influenced, and meanwhile, pathogens can release certain wilting toxins in the colonization process to destroy the immune response of the plants, so that the plants can wilt and die in a short period.

Disease cycles caused by v.dahliae are as follows: (1) the microsclerotia exists on the upper layer or the surface layer of the soil, and can be carried by wind to spread by rainwater; (2) the roots secrete nutrition to stimulate the growth of the roots, the hyphae enter the injured radicles along with water transportation, penetrate through the root crowns and epidermal cells, then penetrate through the cortex and the endothelial layer, and reach xylem vessels after 3 days; (3) the hypha grows to produce conidium which is transported and germinates along with transpiration, and xylem ducts are blocked by invaded and filled bodies and plant colloid to cause diseases such as fallen leaves, wilting, vascular tube discoloration and the like; (4) plant immune response, plant recovery or death; (5) dead tissue produces microsclerotia that induce infection in the next cycle of infection.

In this cycle, there is a close link between disease development and colonization by the pathogen. The plant transports conidium by transpiration pull force, so that the pathogen can be diffused to the whole plant in one week, and the mechanism is proved in elm; the transmission speed is related to plant resistance (physical compression or blockage, blocking the upper lumen of an infected part, generating an invader to immobilize the pathogen; chemical plant protection elements (growth hormone, phytoalexin) or substances such as phenols and tannin, generating blockage, inhibiting hypha growth and spore germination, causing catheter discoloration and defense reaction).

Dahliae hosts are statistically more than 40 families of 660 plants, among which crops (cotton), vegetables (pepper, eggplant, potato, lettuce, etc.), oil plants (olive) and spice plants (pepper), nuts (pistachio), herbs are mainly harmed abroad. In china, the susceptible plants are: cotton, alfalfa, beans, sunflower, cruciferous and solanaceous vegetables, cercis chinensis, maple, clove, camellia, and the like. The forest hosts at home and abroad comprise: maple, catalpa, ailanthus, fruits (apricot, peach, plum, etc.), nut plants, etc.

In order to avoid the economic loss and environmental pollution caused by the infection of forest trees by V.dahliae, the common methods are as follows: selecting plots that are not infested with v.dahliae; selecting nursery stocks, resistant varieties or rootstocks without pathogenic bacteria infection; the fertilizer is not mixed with susceptible forest trees or crops; the polluted land is recovered by methods of soil fumigation, deep turning and insolation, green improvement and the like; enhancing plant quarantine, and well performing sterilization and disinfection before and after planting and pruning; collecting infected plant residues and burning the residues intensively to avoid pathogen transmission.

In the last 70 th century, V.dahliae was controlled by soil fumigation with methyl bromide and trichloronitromethane, and the method was gradually eliminated due to continuous attention on the environment; then, in the mediterranean area, soil deep-turning sun drying is commonly used for preventing and treating the wilt disease of the olive tree caused by V.dahliae; the current green method is to utilize biological fermentation and soil sterilization to prevent and treat. In addition, field management is strengthened, water and fertilizer are reasonably controlled, and the concentrated burning of the trimmed branches and dead plants is also an effective measure for preventing the disease from spreading, but the method has the characteristics of certain limitation, difficult implementation and the like. Therefore, the low-toxicity, green and degradable antibacterial agent has the advantages of low cost, quick response, long control time and the like, and is widely used for blight caused by V.dahliae, including hymexazol, chlorothalonil, carbendazim and the like.

Various pesticides with good effect and low cost for verticillium wilt are also researched and developed by various agriculture and forestry research units and colleges at present, and the application amount and the application method are researched; in view of environmental problems, agents made of microorganisms and metabolites thereof have recently been the focus of research on the control of such diseases as biological control means, and bacillus (bacillus spp.), aspergillus spp, Trichoderma (Trichoderma spp) and partially nonpathogenic fusarium have been used as antagonistic bacteria in crops such as tomatoes, potatoes and eggplants. However, the systemic wilt of acer truncatum has high spreading speed, the disease is not easy to find and difficult to recover, the existing medicament has unobvious effect in the prevention and treatment, the medicament loss is fast, and the medicament effect time is short.

Therefore, the invention provides a fungus composite bactericide which is suitable for treating acer truncatum wilt caused by V.dahliae, aims at the mountainous areas lack of water and drought, and has slow medicament loss and long-lasting medicament effect time, and solves the problems of urgent need of technical personnel in the field.

Disclosure of Invention

In view of the above, the composite antibacterial agent disclosed by the invention aims at the characteristics of high spreading and diffusion speed, difficulty in disease detection, difficulty in recovery, difficulty in treatment and the like of acer truncatum wilt caused by a soil-borne pathogen Verticillium dahliae, utilizes a biological source medicament of shenqinmycin, combines organic silicon and polyacrylamide to carry out composite proportioning, has an internal absorption effect far superior to other medicaments, can kill pathogens colonized at roots and vascular tissues, and effectively controls the disease state; the water retention and synergistic components can reduce water and medicament loss, reduce application times and labor input, and are particularly suitable for water-deficient and drought areas.

In order to achieve the purpose, the invention adopts the following technical scheme:

a composite antimicrobial agent comprising a solid medicament and a liquid medicament; wherein the solid medicament comprises 0.1-1g/kg of polyacrylamide, and the balance of nutrient soil; wherein the liquid medicament comprises 0.5-1.5ml/kg of 1% shenqinmycin; 0.25-1.5ml/kg of organosilicon, and the balance of sterile water.

Further, the solid medicament comprises 0.5-1g/kg of polyacrylamide, and the balance is nutrient soil; wherein the liquid medicament comprises 1-1.5ml/kg 1% shenqinmycin; 0.5-1.25ml/kg of organic silicon, and the balance of sterile water

The shenqinmycin is a secondary metabolite of pseudomonas fluorescens M18 strain, is used as a biological pesticide, and has the advantages of safety, greenness and the like; the agricultural organic silicon has super spreadability, excellent permeability and high-efficiency absorption and conductivity; easy mixing, simple operation, safety, reliability and strong stability. Can promote the penetration of systemic medicament, reduce the pesticide loss, improve the pesticide utilization rate and the rain-resistant scouring force; agricultural polyacrylamide: the polyacrylamide is used as a soil structure modifier, can increase the cohesive force among soil surface layer particles, maintains a good soil structure, prevents soil crusting, increases soil moisture infiltration, inhibits soil moisture evaporation, prevents water and soil loss, and has the effects of water retention, soil conservation, fertilizer conservation, yield increase and the like; the application of polyacrylamide in agricultural production in arid and semiarid regions has important significance in improving the utilization efficiency of irrigation water and natural rainfall, saving water for irrigation and the like.

The biological source preparation shenqinmycin is utilized, organic silicon and polyacrylamide are combined, the absorption of the medicament is enhanced, the medicament loss is reduced, the water retention and synergism are realized, the water retention and fertilizer retention are realized, the medicament effect is enhanced, the quick recovery and normal growth of trees are promoted, the advantages of quick response, long validity period, simple and convenient operation and the like are realized, the used components are common agricultural products, the price is low, the composite proportioning achieves three purposes, and the effect which cannot be compared with the traditional single preparation is realized.

The compound antibacterial agent is applied to acer truncatum wilt caused by V.dahliae.

A method for using a composite antimicrobial agent, comprising the steps of:

(1) the branches and leaves of acer truncatum wilt disease-causing plants caused by V.dahliae are re-cut;

(2) mixing the polyacrylamide and the nutrient soil, and spreading the mixture into crown widths of roots of the diseased plants with branches and leaves pruned in the step (1), and uniformly turning and mixing;

(3) and (3) mixing the 1% shenqinmycin, the organic silicon and the sterile water, and irrigating the mixture in the crown width of the root of the diseased plant in the step (2).

The use method of the composite antibacterial agent disclosed by the invention can effectively prevent and treat acer truncatum wilt caused by verticillium dahliae (V.dahliae); the effect of improving the soil structure is better in the actual field control; meanwhile, the mode of multiple times of pesticide application in the traditional pesticide application process is changed, and the pesticide application is carried out once. The application method disclosed by the invention not only increases the drug effect and reduces the dosage of the drug, but also can ensure that the plants are separated from the disease course and grow well, and reduces the investment of capital and labor force to a great extent.

Further, the acer truncatum diseased tree in the acer truncatum wilt disease caused by V.dahliae is characterized in that the using amount of the solid medicament in the outdoor field is 1-2 kg; the dosage of the liquid medicament is 10-15 kg.

Further, the using amount of the solid medicament for the indoor potting of the acer truncatum diseased tree in the acer truncatum wilt disease caused by V.dahliae is 0.2-0.5 kg; the dosage of the liquid medicament is 2-3 kg.

The invention provides a method for using a composite antibacterial agent; the method can be carried out by one-time pesticide application, and the influence on the normal growth of plants and the product quality caused by the change of pesticide residues, soil structures and soil microbial communities caused by the excessive application times of the traditional chemical agents is completely avoided, so that the method has important significance for ensuring the production of nervonic acid by acer truncatum kernels.

Further, the nutrient soil in the step (2) is sterilized nutrient soil.

Sterilizing the nutrient soil to kill a large amount of germ spores, hyphae, pest eggs, pests and nematodes. Destroying, inactivating, reducing or removing all microorganisms, pollutants and toxins in the soil that may cause acer truncatum to infect blight.

According to the technical scheme, compared with the prior art, the composite antibacterial agent and the use method thereof provided by the invention have the following technical advantages:

(1) aiming at the characteristics of high spreading speed of systemic blight, difficulty in disease detection and recovery and treatment, the provided composite antibacterial agent has a far better internal absorption effect than other agents, can kill pathogens colonized at roots and vascular tissues, and effectively controls the disease state; the water retention component can better retain water and the medicament, reduce water and medicament loss, reduce application times and labor input, and is particularly suitable for water-deficient and drought areas;

(2) the invention provides a method for using a composite antibacterial agent, which changes the mode of applying a small amount of medicine for many times in the traditional medicine application process and can be applied once. The application method disclosed by the invention not only increases the drug effect and reduces the dosage of the drug, but also can ensure that the plants are separated from the disease course and grow well, and reduces the investment of capital and labor force to a great extent.

(3) The invention provides a compound antibacterial agent which can effectively prevent and treat acer truncatum wilt caused by verticillium dahliae; the fertilizer can retain water and fertilizer, enhance the drug effect, promote the rapid recovery and normal growth of trees, has the advantages of quick response, long validity period, simple and convenient operation and the like, all the used components are common agricultural products, the price is low, and the compound proportion achieves three purposes.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a drawing showing the dominant bacterial strain isolated from the vascular tissue of discolored tissue according to the present invention;

FIG. 2 is a drawing showing the disease symptoms of 5-year-old plants inoculated with pathogens (48 d)

Wherein A is the disease condition after inoculating the sterile water and the pathogenic bacteria spore liquid for 48 d; b and C are the leaf characteristics of healthy and diseased plants respectively;

FIG. 3 is a diagram showing the control effect of two bactericides benomyl and shenqinmycin provided by the invention;

wherein A and B are benomyl control effect; c and D are shenqinmycin control effect;

FIG. 4 is a drawing of a 3-year old acer truncatum wilt plant provided by the present invention;

wherein 1 is a 3-year-old acer truncatum wilt disease plant, 2 is a 3-year-old acer truncatum wilt disease plant, and 3 is a healthy acer truncatum plant;

FIG. 5 is a drawing of a 3-year old Acer truncatum plant 60 days after application of the present invention;

wherein 1 is a healthy plant not infected with pathogen, 2 is a group using the complex antimicrobial agent of example 3, 3 is a group using the complex antimicrobial agent of comparative example 2, 4 is a control disease group not treated after the disease is infected, 5 is a group using the complex antimicrobial agent of example 5, and 6 is a group using the complex antimicrobial agent of comparative example 1;

FIG. 6 is a drawing of the whole outdoor infected plant provided by the present invention;

FIG. 7 is a drawing of a plant leaf part affected outdoors according to the present invention;

FIG. 8 is a drawing of an outdoor susceptible plant trunk provided by the present invention;

FIG. 9 is a drawing showing the growth state of outdoor susceptible plants provided by the invention after being applied for 30 days.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Acer truncatum wilt pathogen screening and identification

Collecting the plants with diseases, and taking the plants back to a laboratory for pathogen separation. Tissue separation and dilution coating are carried out on different parts (roots, phloem, xylem and discolored vascular tissue with typical disease symptoms) of acer truncatum diseased plants, bacteria are used for picking single colony by an inoculating loop for streak purification, and fungi are used for picking hypha by a picking needle for purification.

After tissue isolation and dilution coating, 91 strains of fungi 30 strains and bacteria 61 strains are obtained. The acer truncatum is numbered in an order of Acer truncatum name abbreviation (At), according to colony morphology and microscopic observation, 15 strains with the highest occurrence frequency are used for subsequent inoculation experiments, At-13 is obtained by separating a discoloration region of a trunk xylem of all diseased plants through a tissue separation method, and the separation rate is high. Among them, the dominant strains isolated from the color-changed vascular tissue are shown in FIG. 1.

Inoculating the 15 strains with high separation rate in a greenhouse for 5 years, inoculating 1 strain of Acer truncatum Maxim At 42d, and allowing At-13 to have leaf atrophy, no leaf loss, and wilting and drooping symptoms of tender branches; then the whole leaf withered but does not fall off, and the 48 th death after inoculation is shown in figure 2; similar symptoms appear in the other two strains at 45d, the 54d death occurs, and the morbidity reaches 100 percent; the cross section of the base part of the main part of the dead plant has a remarkable brown or dark green discoloring ring, the cross section of the lateral branch is gray black, the root part and the xylem of the main part are gray black to gray black, and the acer truncatum plant inoculated with other strains and a control group do not show any symptoms. The vascular tissue is subjected to tissue isolation to obtain colonies with the same morphology as At-13. Inoculating 3-year-old acer truncatum plants by root again irrigation under outdoor conditions, wherein 3 plants have diseases in 35d, and two plants die in 42d after inoculation; four additional strains developed disease after 60 days of inoculation and died after one week; the rest 3 plants are attacked At 70d after inoculation, the 78 th plants are killed, the morbidity and the mortality are both 100%, and the stems of the attacked plants can be separated to obtain the same strain of At-13, so that the At-13 is determined to be the pathogenic bacteria of the acer truncatum wilt.

The morphological identification is that an At-13 strain is subjected to multiple transfer culture, a bacterial colony is white in the initial growth stage on a PDA culture medium, hyphae are compact, the bacterial colony is changed into grey black after about 5 days, the bacterial colony is mostly black in the later stage, is nearly circular, has smooth edges, is light-colored and nearly transparent, has wrinkles in a center micro-process, and mostly has obvious concentric ring veins with black or grey black alternation, the hyphae are sparse and flocculent in the later stage and have no radial stripes, when the bacterial colony is observed on the back side of the light, conidiophore single cells with black and white alternate annular stripes are formed, are oblong, are not separated, are colorless and transparent, under a microscope, the conidiophore single cells can be seen, two ends of the conidiophore are obviously dark oil balls, the conidiophore single cells are gathered into sticky drops At the top ends of the conidiophore, the size is 2.26-5.001 × 1.458.458-2.96 mu m, most of the conidiophore single cells are thin, the base parts of the conidiophore single cells are slightly expanded, mostly consist of 2-3 layers of wheel branches and top branches or 1 layer of top branches and top branches, each layer of 2-4 branches of grape-shaped branches, the conidiophore of the brown grape-shaped micro-shaped branches are completely combined with the corresponding fungal culture medium, the third half-type fungal culture medium is changed into a white fungus classification, the third sub-type fungal classification of the third.

Molecular biological identification: the CTAB method extracts the total DNA of pathogenic bacteria, amplifies the ITSrDNA sequence by using the fungus universal primer ITS1/4, and carries out sequence comparison by using Blast tool in GenBank database, and the result shows that: identification 521/521 (100%), Gaps 0/521 (0%), combined with morphological identification, finally identified the pathogen as Verticillium dahliae. The GenBank accession number of the gene database is MF149108, the pathogen is preserved in China Center for Type Culture Collection (CCTCC), and the strain number is AF2017015At-13Verticillium dahliae.

Example 2

Antagonism of common v.dahliae control agents

Wherein, the common V.dahliae preventive agents are shown in Table 1

Table 1 common v.dahliae control agents

Preparing the above medicines into a toxic plate with final concentration of 0.005-1000 μ g/mL, inoculating pathogenic bacteria hypha blocks, coating about 120 spores/50 μ L of spore solution, counting the inhibition effect of different medicine concentrations on the hypha growth and spore germination of pathogenic bacteria, and calculating the EC of each medicine₅₀。

The data analysis results and the toxicity regression equation of the growth inhibition of pathogenic bacteria by different concentrations of the 7 medicaments are shown in tables 2 and 3, the medicaments with better antibacterial effect are the shenqinmycin and benomyl, the low concentration shows stronger inhibition capability and EC₅₀0.3281 and 0.4966. mu.g/mL, respectively; the Ningnanmycin, chlorothalonil and the like have poor inhibition effect on At-13.

TABLE 2 inhibition rate of the fungicides on At-13 hypha growth

TABLE 3 regression equation of virulence and EC of fungicides on At-13 hypha growth₅₀

The germination inhibition effect of the pathogenic bacteria At-13 spores after being cultured for 60 hours At 25 ℃ in pesticides with different concentrations is shown in tables 4 and 5, the germination inhibition effect of different medicaments on the pathogenic bacteria spores is different, and the germination time of all medicament treatments is obviously delayed compared with the control 48 hours; the concentrations of the agents for inhibiting spore germination are all lower than the concentration of the agents for inhibiting hypha; consistent with the result of hypha growth inhibition, the shenqinmycin and benomyl have better inhibition effect on spore germination of pathogenic bacteria, and the EC of the shenqinmycin and benomyl₅₀Are respectively 0.0551 and 0.0035. mu.g/mL; the other effects are as follows in sequence: hymexazol, thiophanate methyl, mancozeb and ningnanmycin, wherein the ningnanmycin has the same inhibition result with hypha, and has an inhibition effect when the concentration is high; chlorothalonil has no good inhibition effect on spore germination of pathogenic bacteria.

TABLE 4 inhibition of At-13 spore germination by fungicides

TABLE 5 regression equation of virulence and EC of fungicides on At-13 spore germination₅₀

Taking 1000 times of shenqinmycin solution and 600 times of benomyl solution as the concentrations of the control agents, pre-applying 2 effective agents, inoculating spore solutions at 7d and 15d, and simultaneously inoculating the spore solution (0d) as a defense group; the agent was applied as treatment groups at 7d, 15d and 30d after inoculation of spore fluid, 10 in each group, and the control effect was observed at 60d as shown in fig. 3 and table 6. The data in the table show that the plants inoculated with the pathogenic bacteria spore liquid all die, the clear water control group is healthy, after 15 days of shenqinmycin is applied, the plants have better defense effect on the pathogens, and the plants applied with benomyl all die; when the disease is infected initially before the disease, the 2 medicaments have good treatment effect on the disease, and when the disease is colonized and diffused in the trunk of a plant (the disease has no external expression), the shenqinmycin can also be used for treatment, while the benomyl cannot inhibit the diffusion of the disease, and in the later period of the disease course, the shenqinmycin can be used for treating part (about 40%) of infected plants.

TABLE 6 prevention and treatment of acer truncatum wilt with two agents

The experimental results show that only shenqinmycin shows good defense effect among 7 bactericides for preventing and treating verticillium dahliae, but in the aspect of treatment, when disease characteristics are shown, shenqinmycin cannot achieve good treatment effect, and cannot inhibit pathogen transmission and reduce loss in the process of large-area planting. Aiming at the characteristics that the acer truncatum wilt is not easy to observe and dies quickly, the following effects can be achieved through the hope of compounding the medicament: the systemic effect of the pesticide is enhanced, the pesticide loss is reduced, the effective period of the pesticide in soil is prolonged, and the labor cost is reduced for water shortage and large-area application in mountainous areas.

Example 3

A composite antibacterial agent comprises a solid agent and a liquid agent; wherein the solid medicament comprises 0.5g/kg of polyacrylamide, and the balance is nutrient soil; wherein the liquid medicament comprises 1ml/kg of 1% shenqinmycin; 1ml/kg of silicone, the balance being sterile water.

1% shenqinmycin medicine of Shanghai Nongle is adopted to carry out 3-year indoor pot culture control on acer truncatum wilt, and 10 plants are planted in each group. Wherein, 3 years old acer truncatum wilt plants are shown in figure 4.

The branches and leaves of the acer truncatum disease plant in the acer truncatum wilt disease caused by V.dahliae are re-sheared; the dosage of the solid medicament is 0.2 kg; the using amount of the liquid medicament is 3kg, wherein 0.1g of polyacrylamide is mixed with 199.9g of sterilized nutrient soil and is scattered on the roots of the diseased potted plants; and (3) mixing 3ml of 1% shenqinmycin with 3ml of organic silicon, adding sterile water to reach 3kg, and irrigating the roots of the diseased potted plants in the step (2) at one time. After 60 days, the effect is shown in table 7 and fig. 5.

Example 4

A composite antibacterial agent comprises a solid agent and a liquid agent; wherein the solid medicament comprises 0.25g/kg of polyacrylamide, and the balance of nutrient soil; wherein the liquid medicament comprises 1.5ml/kg of 1% shenqinmycin; 0.5ml/kg of silicone, the balance being sterile water.

The 1% shenqinmycin medicine of Shanghai Nongle is adopted to carry out indoor control on 3-year acer truncatum wilt, and 10 plants are in each group.

The branches and leaves of the acer truncatum disease plant in the acer truncatum wilt disease caused by V.dahliae are re-sheared; the dosage of the solid medicament is 0.4 kg; the using amount of the liquid medicament is 2kg, wherein 0.1g of polyacrylamide is mixed with 399.9g of sterilized nutrient soil and is scattered on the roots of the diseased potted plants; and (3) mixing 3ml of 1% shenqinmycin with 1ml of organic silicon, adding sterile water to reach 2kg, and irrigating the roots of the diseased potted plants in the step (2) at one time. After 60 days, the effect is shown in table 7 and fig. 5.

Example 5

A composite antibacterial agent comprises a solid agent and a liquid agent; wherein the solid medicament comprises 1g/kg of polyacrylamide, and the balance of nutrient soil; wherein the liquid medicament comprises 1% shenqinmycin at a concentration of 0.5 ml/kg; 1ml/kg of silicone, the balance being sterile water.

The branches and leaves of the acer truncatum disease plant in the acer truncatum wilt disease caused by V.dahliae are re-sheared; the dosage of the solid medicament is 0.5 kg; the using amount of the liquid medicament is 2.4kg, wherein 0.5g of polyacrylamide is mixed with 499.5g of sterilized nutrient soil and is scattered on the roots of the diseased potted plants; and (3) mixing 1.2ml of 1% shenqinmycin with 2.4ml of organic silicon, adding sterile water to reach 2.4kg, and irrigating the roots of the diseased potted plants in the step (2) at one time. After 60 days, the effect is shown in table 7 and fig. 5.

Example 6

A composite antibacterial agent comprises a solid agent and a liquid agent; wherein the solid medicament comprises 0.1g/kg of polyacrylamide, and the balance is nutrient soil; wherein the liquid medicament comprises 1.2ml/kg of 1% shenqinmycin; 0.25ml/kg of silicone, the remainder being sterile water.

The branches and leaves of the acer truncatum disease plant in the acer truncatum wilt disease caused by V.dahliae are re-sheared; the dosage of the solid medicament is 0.2 kg; the using amount of the liquid medicament is 3kg, wherein 0.02g of polyacrylamide is mixed with 199.98g of sterilized nutrient soil and is scattered on the roots of the diseased potted plants; and (3.6 ml of 1% shenqinmycin is mixed with 0.75ml of organic silicon, and then sterile water is added to reach 3kg, and the roots of the diseased potted plants in the step (2) are irrigated at one time. After 60 days, the effect is shown in Table 7.

Example 7

A composite antibacterial agent comprises a solid agent and a liquid agent; wherein the solid medicament comprises 0.6g/kg of polyacrylamide, and the balance is nutrient soil; wherein the liquid medicament comprises 1% shenqinmycin at a concentration of 0.6 ml/kg; 1.5ml/kg of silicone, the remainder being sterile water.

The branches and leaves of the acer truncatum disease plant in the acer truncatum wilt disease caused by V.dahliae are re-sheared; the dosage of the solid medicament is 0.2 kg; the using amount of the liquid medicament is 3kg, wherein 0.12g of polyacrylamide is mixed with 199.88g of sterilized nutrient soil and is scattered on the roots of the diseased potted plants; and (3) mixing 1.8ml of 1% shenqinmycin with 4.5ml of organic silicon, adding sterile water to reach 3kg, and irrigating the roots of the diseased potted plants in the step (2) at one time. After 60 days, the effect is shown in Table 7.

Comparative example 1

A composite antibacterial agent comprises a solid agent and a liquid agent; wherein the solid medicament comprises 1.5g/kg of polyacrylamide, and the balance of nutrient soil; wherein the liquid medicament comprises 1% shenqinmycin at a concentration of 0.1 ml/kg; 2ml/kg of silicone, and the balance of sterile water.

The branches and leaves of the acer truncatum disease plant in the acer truncatum wilt disease caused by V.dahliae are re-sheared; the dosage of the solid medicament is 0.2 kg; the using amount of the liquid medicament is 3kg, wherein 0.3g of polyacrylamide is mixed with 199.7g of sterilized nutrient soil and is scattered on the roots of the diseased potted plants; and (3) mixing 0.3ml of 1% shenqinmycin with 6ml of organic silicon, adding sterile water to reach 3kg, and irrigating the roots of the diseased potted plants in the step (2) at one time. After 60 days, the effects are shown in table 7 and fig. 5.

Comparative example 2

A composite antibacterial agent comprises a solid agent and a liquid agent; wherein the solid medicament comprises 0.4g/kg of polyacrylamide, and the balance is nutrient soil; wherein the liquid medicament comprises 1ml/kg of 1% shenqinmycin; 1ml/kg of silicone, the balance being sterile water.

The branches and leaves of the acer truncatum disease plant in the acer truncatum wilt disease caused by V.dahliae are re-sheared; the dosage of the solid medicament is 0.2 kg; the using amount of the liquid medicament is 3kg, wherein 0.08g of polyacrylamide is mixed with 199.92g of sterilized nutrient soil and is scattered on the roots of the diseased potted plants; and (3) mixing 3ml of 1% shenqinmycin with 3ml of organic silicon, adding sterile water to reach 3kg, irrigating the roots of the diseased potted plants in the step (2) for 3 times, and observing after 60 days, wherein the roots are irrigated 1 time every 5 days, and the effect is shown in the table 7.

Comparative example 3

1 percent of shenqinmycin medicine of Shanghai Nongle is adopted to prevent and treat the acer truncatum wilt of acer truncatum in the four village base of acer truncatum of Shandong Yongchuntang Limited company. And (3) carrying out heavy shearing on branches and leaves of the acer truncatum disease tree in the acer truncatum wilt disease caused by V.dahliae, irrigating roots within the range of the crown width of the root of the disease tree, wherein the dosage of each plant is 10kg, and 30 plants are irrigated at one time to the root of the potted plant diseased in the step (2), and the effect is shown in the table 7 and the figure 5.

Table 7 growth of the diseased potted plants 60 days after dosing.

From Table 7, it can be seen that the compound preparation not only can restore normal growth of plants, but also can significantly reduce the mortality rate after diseases, and the effect is obviously better than that of a single preparation. The drug effect is enhanced, the effective period is prolonged, and the manual input is reduced.

Example 8

Carrying out acer truncatum wilt prevention and control on northern mountain bases of Shandong Sishui county Shandong Yongchuntang finite company, Kun Maolanzi gardens of Shandong Qufu City and Yuanzhu Yuanbao maple bases of Shandong Qufu City, wherein the growth states of acer truncatum wilt plants are shown in figures 6-8, carrying out re-shearing on branches and leaves of acer truncatum diseased trees in the acer truncatum wilt disease caused by V.dahliae according to the compound antibacterial agent in the example 3, wherein the dosage of each acer truncatum tree is 15kg and 30 trees are all used, the diseased trees are irrigated in root crowns of the diseased plants at one time for prevention and control, and new buds are observed after 30 days and grow well as shown in figure 9; in spring of the next year, the diseased trees grew vigorously, and 3 plants were randomly selected to determine the effect, and the results are shown in Table 8.

TABLE 8 control Effect on different plots

The invention provides a compound antibacterial agent, aiming at the characteristics of high spreading speed of systemic wilt, difficult disease detection and difficult recovery and treatment, etc., the compound proportioning is carried out by utilizing the shenqinmycin in combination with organic silicon and polyacrylamide, the internal absorption effect of the agent is far better than that of other agents, the agent can kill pathogens colonized at roots and vascular tissues, and the disease condition can be effectively controlled; the water retention component can better retain water and the medicament and reduce the loss of the water and the medicament; the application method of the composite antibacterial agent disclosed in the publication effectively reduces the application times and labor input, and is particularly suitable for water-deficient and drought areas.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composite antimicrobial agent, comprising a solid agent and a liquid agent; wherein the solid medicament comprises 0.1-1g/kg of polyacrylamide, and the balance of nutrient soil; wherein the liquid medicament comprises 0.5-1.5ml/kg 1% shenqinmycin; 0.25-1.5ml/kg of organosilicon, and the balance of sterile water.

2. The composite antibacterial agent according to claim 1, wherein said solid dosage form comprises 0.5-1g/kg of polyacrylamide, and the balance being nutrient soil; wherein the liquid medicament comprises 1-1.5ml/kg 1% shenqinmycin; 0.5-1.25ml/kg of organosilicon, and the balance of sterile water.

3. The use of a complex antibacterial agent according to claim 1 in acer truncatum wilt disease caused by v.

4. The method for using the composite antibacterial agent according to claim 1, characterized by comprising the following steps:

(1) re-shearing branches and leaves of acer truncatum wilt disease-causing plants caused by V.dahliae;

(3) mixing the 1% shenqinmycin, the organic silicon and the sterile water, and irrigating the mixture in the crown width of the root of the diseased plant in the step (2);

the acer truncatum diseased tree in the acer truncatum wilt disease caused by V.dahliae is characterized in that the using amount of an outdoor field solid medicament is 1-2 kg; the dosage of the liquid medicament is 10-15 kg;

the acer truncatum diseased tree in the acer truncatum wilt disease caused by V.dahliae is characterized in that the use amount of an indoor potted solid medicament is 0.2-0.5 kg; the dosage of the liquid medicament is 2-3 kg.

5. The use method of compound antibacterial agent as claimed in claim 4, wherein said nutrient soil in step (2) is sterilized nutrient soil.