CN108812110B - Method for identifying temperature sensing characteristics of brassica napus by using relative plant height of plants - Google Patents
Method for identifying temperature sensing characteristics of brassica napus by using relative plant height of plants Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention relates to a method for identifying temperature sensing characteristics of cabbage type rape by using relative plant height of plants, which comprises the following steps of 1) sowing a cabbage type rape variety to be identified in autumn in a field; 2) film coating treatment(ii) a Measuring the plant heights h of all cabbage type rape varieties to be identified after autumn sowing in a field when the rape is in the full-bloom stage; 3) calculating the average plant height of the autumn sowing plants in the field according to h
4) Carrying out field spring sowing, and measuring the plant heights h' of all plants in the cabbage type rape variety group after the cabbage type rape seedlings emerge after the field spring sowing; 5) according to the plant height h' of the spring sowing plant and the average plant height of the field autumn sowing plant
Description
Technical Field
The invention belongs to the technical field of plant cold resistance type identification, and relates to a method for identifying temperature sensing characteristics of brassica napus by using relative plant heights of plants.
Background
The northern cold and arid regions are arid and rainless all year round, cultivated lands in winter and spring are idle, the vegetation coverage rate is low, sand storms and dusty weather occur frequently in early spring of the next year, the life of people is affected, and particularly, the damage to the agricultural ecological environment is more remarkable. The cabbage type rape is planted in a northern cold arid region, so that the ecological environment is protected, the vegetation coverage rate in winter and spring in the north is obviously increased, the breeding of the cold-resistant rape variety is the key for developing the production of the northern winter rape under the northern severe climate condition, and the breeding of the cold-resistant variety firstly solves the problem of a simple, effective and low-cost cold-resistant identification method.
The conventional cold resistance identification adopts identification methods such as field overwintering rate, physiological and biochemical methods and the like. The statistics of the field overwintering rate cannot accurately judge the cold resistance of a single plant, and the cold resistance is greatly influenced by human factors and external factors; the indexes of the physiological and biochemical methods have high measuring cost and strict requirements on instruments, and most physiological indexes have large and unstable changes in the process.
The patent application number 201610227970.4 discloses a method for identifying the winter character of Chinese cabbage type winter rape by plant height, which comprises planting the Chinese cabbage type winter rape to be identified in the field in late spring and early summer, wherein the total number of seedlings is more than 30; growing for 60-65 days after seedling emergence, and measuring the plant heights of all seedlings in the cabbage type winter rape population to be identified; carrying out plant height standardization; the winterness of each plant is judged according to the plant height standardization. The utilization is only the spring sowing plant height and the maximum plant height (X) in the varietymax) However, the vernalization degree of each variety is different, and the maximum plant height ratio is different, so the method has no correlation characteristics among the varieties in principle, can not be used for comparing the winter property strength of different varieties of materials, and can not be used for comparing and distinguishing the winter and spring property identification of the vernalized varieties.
Disclosure of Invention
In order to solve the technical problems in the background art, the invention provides a method for identifying the temperature sensing characteristic of the brassica napus by using the relative plant height of a plant, which has simple experimental operation and higher accuracy.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for identifying the temperature sensing characteristic of brassica napus by using the relative plant height of a plant is characterized by comprising the following steps: the method for identifying the temperature sensing characteristic of the brassica napus by using the relative plant height of the plant comprises the following steps:
1) sowing the cabbage type rape variety to be identified in the field in autumn; the local elevation of the field is between 1800 and 2200 meters;
2) after the autumn sowing in the field, performing film covering treatment on the brassica napus variety to be identified from 11 months to 3 months of the next year in the current year, and preventing cold damage caused by extreme low temperature; measuring the plant heights h of all cabbage type rape varieties to be identified after autumn sowing in a field until 7 months earlier in the next year when the rape is in full bloom;
3) calculating the average plant height of the autumn sowing plants in the field according to the measured plant height h
The average plant height of the autumn sowing plants in the field
The calculation formula of (2) is:
wherein:
h is the measured plant height in cm;
n is the number of the plant plants when the rape is in the full bloom stage after autumn sowing in the field;
4) carrying out field spring sowing on a cabbage type rape variety which is subjected to field autumn sowing and can be opened in the full-bloom period of rape 7 months next year, wherein the field spring sowing time is 4 months, the average temperature of the field spring sowing is 15 ℃, after the field spring sowing, the seedling emergence growth of the cabbage type rape is not less than 90 days, measuring the plant heights of all plants in a cabbage type rape variety group and marking as spring sowing plant height h', and marking the plant height without bolting as 0;
5) the plant height h' of the spring sowing plant obtained in the step 4) and the average plant height of the field autumn sowing plant obtained in the step 3)
And respectively judging the temperature sensing characteristics of the single plants of the same cabbage type rape variety or judging the temperature sensing characteristics of all cabbage type rape varieties during autumn sowing in a field.
The specific implementation manner of judging the temperature sensing characteristic of the same cabbage type rape variety single plant in the step 5) is as follows:
the plant height h' of the spring sowing plant obtained by measuring according to the step 4) and the average plant height of the field autumn sowing plant obtained by calculating according to the step 3)
Calculating the relative plant height Hc of the single plants of all the cabbage type rape varieties during spring sowing in the field; judging the temperature sensing characteristic of a single plant of the cabbage type rape variety according to the relative plant height Hc of all the cabbage type rape varieties; the specific calculation mode of the relative plant height Hc of the single plants of all the cabbage type rape varieties is as follows:
wherein:
HCis the relative plant height of single plants of all cabbage type rape varieties during spring sowing in the field;
h' is the plant height of the spring sowing plant in cm;
The condition for judging the temperature sensing characteristic of the individual cabbage type rape varieties according to the relative plant height Hc of the individual cabbage type rape varieties during autumn sowing in the field is as follows: hc is 0, and the temperature sensitivity is strongest; hc is more than 0 and less than or equal to 0.2, and the temperature sensitivity is strong; hc is more than 0.2 and less than or equal to 0.4, and the temperature sensitivity is weak; hc is greater than 0.4, and the temperature sensitivity is poor.
The specific implementation manner of judging the temperature sensing characteristics of all cabbage type rape varieties during autumn sowing in the field in the step 5) is as follows:
respectively calculating the average plant height of each cabbage type rape variety sowed in the field in spring
According to the average plant height of the same cabbage type rape variety in spring sowing in the field
And the average plant height of the variety in autumn sowing in the field
Calculating the relative plant height Hs of the variety; judging the temperature sensing characteristic of the current cabbage type rape variety according to the relative plant height Hs of the current variety;
the average plant height of the field sowed in spring
The calculation method of (1) is as follows:
wherein:
h' is the measured plant height of the spring sowing plant in cm;
n is the number of plant plants in the full-bloom period of the rape after the spring sowing of the field;
the calculation mode of the relative plant height Hs of the current variety single plant is as follows:
wherein:
hs is the relative plant height of the current variety;
The judgment condition for judging the temperature sensing characteristic of the current cabbage type rape variety according to the relative plant height Hs of the current variety is as follows: hs is less than 0.2, and has the strongest temperature sensitivity and strong winterness; hs is more than or equal to 0.2 and less than 0.3, and has strong temperature sensitivity and winter property; hs is more than or equal to 0.3 and less than 0.4, and has weak temperature sensitivity and half-winterness; hs is more than 0.4, poor temperature sensitivity and springiness.
The Brassica napus variety to be identified is a combination of a plurality of new oil No. 23, 16TS 306-4 (spring) guining, 16TS306-3 choosen 1 guining, 16TS 312-2 guining, 16TS 309-10 guining, 16NPZ 269-1 mixed selection, 16NPZ 158 mixed selection, 15NS 45-4 mixed selection, 20168 (G) selection, 2016TS (G)10, Tianyou 2266, and Tianyou 2288.
The number of plants of each variety of the cabbage type rape variety to be identified is not less than 30 when the cabbage type rape variety is sowed in the field in autumn.
The number of the cabbage type rape varieties to be identified is N; the total number of the plants sowed in autumn in the field is M; m is more than or equal to 30N + 10.
The condition for measuring the plant heights h of all cabbage type rape varieties to be identified after autumn sowing in the field in the step 2) is that the number of the plants opened in the full-bloom period of the rape is not less than 2/3M; and M is the total number of plants sowed in autumn in the field.
The invention has the advantages that:
the invention provides a method for identifying temperature sensing characteristics of cabbage type rape by using relative plant heights of plants, planting a cabbage type rape variety to be identified in a field in autumn, measuring the plant height of a cabbage type rape variety group which smoothly overwinter in 7 months next year, and calculating the average plant height; then, spring sowing is carried out on the rape varieties which successfully live through the winter, the seedlings grow for 90 days, the heights of all the plants in the rape variety groups are measured, and the height of the single plant which does not bolt is marked as '0'; and calculating the relative plant height of the plant and the relative plant height of the variety, and judging the temperature sensitivity of the single plant and the variety according to the relative plant heights so as to determine the winter performance of the plant. The identification method overcomes the defects of the traditional rape overwintering rate statistics, physiological biochemistry and molecular marking methods, is simple to operate, identifies the winterness of single plants and varieties, and has the advantages of low requirements on laboratory conditions and technologies, low cost and reliable results. The invention judges the temperature sensing characteristics of single plants or varieties of the cabbage type rape by measuring the average plant height of the cabbage type rape group which smoothly passes through the vernalization group after autumn sowing and the plant height of each plant after spring sowing and calculating the relative plant height of the plants so as to further identify the cold resistance of the plants.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
The invention provides a method for identifying the temperature sensing characteristic of cabbage type rape by using the relative plant height of a plant, which comprises the following steps:
1) sowing the cabbage type rape variety to be identified in the field in autumn; the field is a Shang Chuan test base in a Lanzhou new area; the local sea level of the field is between 1800 and 2200 meters; the average rainfall in the year is 231mm, the drought index reaches 5.4, the average annual temperature is 10.3 ℃, the average minimum temperature in the coldest month is about-10 ℃, the extreme low temperature is about-28 ℃, the negative accumulated temperature in winter is-570 ℃, the average annual sunshine hours are 2446h, the frost-free period is 140d, and the annual precipitation is 327 mm; the low-temperature effective accumulation amount can ensure that all cabbage type rapes are vernalized;
2) after the autumn sowing in the field, performing film covering treatment on the brassica napus variety to be identified from 11 months to 3 months of the next year in the current year, and preventing cold damage caused by extreme low temperature; measuring the plant heights h of all cabbage type rape varieties to be identified after autumn sowing in a field until 7 months earlier in the next year when the rape is in full bloom;
3) calculating the average plant height of the autumn sowing plants in the field according to the measured plant height h
Average plant height of field autumn sowing plants
The calculation formula of (2) is:
wherein:
h is the measured plant height in cm;
n is the number of the plant plants when the rape is in the full bloom stage after autumn sowing in the field;
4) carrying out field spring sowing on the cabbage type rape variety which is subjected to field autumn sowing and can be opened in the full-bloom period of rape in the next 7 months, wherein the field spring sowing time is 4 months at the beginning, and the field is a Shang-Chuan test base in a Lanzhou new area; the average temperature of spring sowing in the field is 15 ℃, the average low temperature is 2 ℃, the extreme high temperature is 21 ℃, the extreme low temperature is-4 ℃, a certain amount of low temperature accumulation exists, after the cabbage type rape seedlings grow for not less than 90 days after the spring sowing in the field, the plant heights of all plants in the cabbage type rape variety group are measured and are marked as spring sowing plant heights h', and the plant heights without bolting are marked as 0;
5) the plant height h' of the spring sowing plant obtained in the step 4) and the average plant height of the field autumn sowing plant obtained in the step 3)
Respectively judging the temperature sensing characteristics of the same cabbage type rape variety or all cabbage type rape varieties in autumn sowing in the fieldAnd judging the temperature sensing characteristics.
The specific implementation mode of judging the temperature sensing characteristic of the same cabbage type rape variety single plant in the step 5) is as follows:
the plant height h' of the spring sowing plant obtained by measuring according to the step 4) and the average plant height of the field autumn sowing plant obtained by calculating according to the step 3)
Calculating the relative plant height Hc of the single plants of all the cabbage type rape varieties during spring sowing in the field; judging the temperature sensing characteristic of a single plant of the cabbage type rape variety according to the relative plant height Hc of all the cabbage type rape varieties; the specific calculation mode of the relative plant height Hc of the single plants of all the cabbage type rape varieties is as follows:
wherein:
HCis the relative plant height of single plants of all cabbage type rape varieties during spring sowing in the field;
h' is the plant height of the spring sowing plant in cm;
The judgment condition for judging the temperature sensing characteristic of the single plant of the cabbage type rape variety according to the relative plant height Hc of the single plant of all the cabbage type rape varieties during autumn sowing in the field is as follows: hc is 0, and the temperature sensitivity is strongest; hc is more than 0 and less than or equal to 0.2, and the temperature sensitivity is strong; hc is more than 0.2 and less than or equal to 0.4, and the temperature sensitivity is weak; hc is greater than 0.4, and the temperature sensitivity is poor.
The specific implementation mode of judging the temperature sensing characteristics of all cabbage type rape varieties in the autumn sowing in the field in the step 5) is as follows:
respectively calculating the average plant height of each cabbage type rape variety sowed in the field in spring
According to the same cabbage type rape varietyAverage plant height in spring sowing in field
And the average plant height of the variety in autumn sowing in the field
Calculating the relative plant height Hs of the variety; judging the temperature sensing characteristic of the current cabbage type rape variety according to the relative plant height Hs of the current variety;
the average plant height of the field sowed in spring
The calculation method of (1) is as follows:
wherein:
h' is the measured plant height of the spring sowing plant in cm;
n is the number of plant plants in the full-bloom period of the rape after the spring sowing of the field;
the calculation mode of the relative plant height Hs of the current variety single plant is as follows:
wherein:
hs is the relative plant height of the current variety;
The judgment condition for judging the temperature sensing characteristic of the current cabbage type rape variety according to the relative plant height Hs of the current variety is as follows: hs is less than 0.2, and has the strongest temperature sensitivity and strong winterness; hs is more than or equal to 0.2 and less than 0.3, and has strong temperature sensitivity and winter property; hs is more than or equal to 0.3 and less than 0.4, and has weak temperature sensitivity and half-winterness; hs is more than 0.4, poor temperature sensitivity and springiness.
The brassica napus variety to be identified is a combination of a plurality of new oil number 23, 16TS 306-4 (spring) guining, 16TS306-3 choosen 1 guining, 16TS 312-2 guining, 16TS 309-10 guining, 16NPZ 269-1 co-selection, 16NPZ 158 co-selection, 15NS 45-4 co-selection, 20168 (G) selection, 2016TS (G)10, day oil 2266, and day oil 2288.
The number of plants of each variety is not less than 30 when the cabbage type rape variety to be identified is sowed in the field in autumn; the number of cabbage type rape cultivars to be identified is N; the total number of plants sowed in autumn in the field is M; m is more than or equal to 30N + 10.
And 2) measuring the plant heights h of all the cabbage type rape varieties to be identified after autumn sowing in the field, wherein the condition is that the number of the plants opened in the full-bloom stage of the rape is not less than 2/3M (the full-bloom stage is more than half of the plants in the whole field).
The theoretical basis of the method for identifying the temperature sensing characteristic of the brassica napus by using the relative plant height of the plants provided by the invention is as follows: the sensitivity of rapes with different cold resistance and different hereditary types to temperature is different, the stronger the cold resistance, the stronger the winterness, and the lower the temperature required for vernalization, the stronger the temperature sensitivity. After the cabbage type rape is sowed in autumn, the overwintering conditions of rape variety groups with different cold resistance are different at a lower temperature after a certain time; and selecting a variety group which successfully overwinter, sowing in spring, and further identifying the temperature sensitivity of the variety group.
The working principle of the invention is as follows: after spring sowing, in the same natural environment, due to different temperature sensitivity of variety groups, the plant height difference of plants is obvious, and due to insufficient low-temperature accumulation through vernalization, the variety groups with strong temperature sensitivity cannot show buds and bolt, and the plant height is small; and the variety group with weak temperature sensitivity can show buds, blossoms and seeds after vernalization after spring sowing, and the plant height is higher. According to the influence of the temperature sensitivity of the plants on the height of the plants, the strength of the group temperature sensitivity can be identified, and the cold resistance of the group temperature sensitivity can be judged.
Example (b): 2016 experiment in Shang Chuan town of Lanzhou
1) Sowing in autumn in the field: the cabbage type rape variety to be identified is planted in a field of a Shanchuan test base in a new Lanzhou area, the local altitude is between 1800 plus 2200 meters, the annual average rainfall in the interior is 231mm, the drought index reaches 5.4, the annual average temperature is 10.3 ℃, the average coldest month average lowest temperature is about-10 ℃, the extreme low temperature is about-28 ℃, the negative accumulated temperature in winter is-570 ℃, the annual average sunshine duration is 2446h, the frost-free period is 140d, and the annual rainfall is 327 mm; the low-temperature effective accumulation amount can ensure that the total number of seedlings planted by the rapes is over 100 through vernalization of all the cabbage-type rapes, so that the accuracy of an experimental result is improved;
2) the rape to be identified is subjected to film covering treatment from 11 months to 3 months in the next year, so that cold damage caused by extreme low temperature is prevented; measuring all plant heights of the fall-sown rape variety group at the full-bloom stage of the rape (the full-bloom stage is more than half of the plants in the whole field, and the branched flowers of 2/3 are opened) by the beginning of 7 months;
3) calculating according to the measured plant height:
the average plant height of autumn sowing plants is unit (cm); hi is the plant height of any autumn sowing plant in unit (cm); n is the number of plant strains.
4) Sowing in spring in a field: spring sowing the cabbage type rape variety group which is sowed in autumn and safely overwintering in the field at the beginning of 4 months, wherein the average high temperature of the cabbage type rape variety group in the Shanchuan test base is 15 ℃, the average low temperature is 2 ℃, the extreme high temperature is 21 ℃ and the extreme low temperature is-4 ℃; there is a certain amount of low temperature build-up. Measuring the heights of all the plants in the rape variety group after the seedlings of the rapes grow for 90 days, and recording the height of the plant which does not bolt as 0;
5) according to the measured plant height and the average plant height of autumn sowing
In the formula: HC is the relative plant height of a single rape plant, h is the plant height of a spring sowing plant in cm;
6) judging the temperature sensing characteristic of the single cabbage type rape plant: hc is 0, the temperature sensitivity is strongest, and the winterness is strong; hc is 0, the temperature sensitivity is strongest, and the winterness is strong; hc is more than 0 and less than or equal to 0.2, and the cold-feeling property and the winter property are strong; hc is more than 0.2 and less than or equal to 0.4, and the cold-sensing property and the half-winter property are weak; hc is greater than 0.4, poor temperature sensitivity and springiness.
7) Calculating the temperature sensing characteristic of the cabbage type rape variety: calculating the average plant height of the spring sowing plants in any variety of the cabbage type rape
The average plant height (cm) of any variety in spring sowing; hi' is the plant height of any variety of spring sowing plants in unit (cm); n is the number of plant strains.
8) According to the average plant height of a certain variety of spring sowing plants
The average plant height of rape variety group in autumn sowing
The relative plant height of the cultivar is calculated and expressed as HS, i.e.
HS is the relative plant height of rape variety.
9) Judging the temperature sensing characteristics of the cabbage type rape varieties: hs is less than 0.2, and the winterness is strong; hs is more than or equal to 0.2 and less than 0.3, and the winterness is good; hs is more than or equal to 0.3 and less than 0.4, and the winter character is half winter; hs is more than 0.4, and springiness.
TABLE 1 relative plant height of variety
See table 1, there are a total of 12 brassica napus varieties and are numbered; the average plant height of autumn sowing refers to measuring all plant heights of rape seed groups of autumn sowing in winter after the rape to be identified is sowed in autumn and 7 months next year in full-bloom period of the rape, namely when branch flowers of over 240 rape plants in the whole field are opened, and obtaining the average value, namely the average plant height of the autumn sowing; similarly, the average plant height in autumn sowing means that rape to be identified is sown at the beginning of 4 months, the plant heights of all plants in a rape variety group are measured after rape seedlings grow for 90 days, and the plant height without bolting is marked as '0'; the relative plant height of the variety is determined by the specific value of the spring sowing plant height to the autumn sowing plant height, and the temperature sensitivity of the variety is determined according to the specific value of the variety plant height. Referring to table 2, for the individual plant heights of each of the 12 brassica napus varieties, 27 individual plants were measured for each variety, and the height of the plant that did not bolt was designated as "0"; and recording the height of the other bolting and bud-appearing plants as h and unit cm.
TABLE 2 mean plant height for spring sowing of different varieties
Claims (9)
1. A method for identifying the temperature sensing characteristic of brassica napus by using the relative plant height of a plant is characterized by comprising the following steps: the method for identifying the temperature sensing characteristic of the brassica napus by using the relative plant height of the plant comprises the following steps:
1) sowing the cabbage type rape variety to be identified in the field in autumn; the local elevation of the field is between 1800 and 2200 meters;
2) after the sowing in autumn in the field, the cabbage type rape variety to be identified is subjected to film covering treatment from 11 months to 3 months of the next year, so that cold damage caused by extreme low temperature is prevented; measuring the plant heights h of all cabbage type rape varieties to be identified after autumn sowing in a field until 7 months earlier in the next year when the rape is in full bloom;
3) calculating the average plant height of the autumn sowing plants in the field according to the measured plant height h
The average plant height of the autumn sowing plants in the field
The calculation formula of (2) is:
wherein:
h is the measured plant height in cm;
n is the number of the plant plants when the rape is in the full bloom stage after autumn sowing in the field;
4) carrying out field spring sowing on a cabbage type rape variety which is subjected to field autumn sowing and can be opened in the full-bloom period of rape 7 months next year, wherein the field spring sowing time is 4 months, the average temperature of the field spring sowing is 15 ℃, after the field spring sowing, the seedling emergence growth of the cabbage type rape is not less than 90 days, measuring the plant heights of all plants in a cabbage type rape variety group and marking as spring sowing plant height h', and marking the plant height without bolting as 0;
5) the plant height h' of the spring sowing plant obtained in the step 4) and the average plant height of the field autumn sowing plant obtained in the step 3)
And respectively judging the temperature sensing characteristics of the same cabbage type rape variety or all cabbage type rape varieties during autumn sowing in the field.
2. The method for identifying the temperature-sensitive characteristics of Brassica napus by using the relative plant height of plants as claimed in claim 1, wherein: the specific implementation mode for judging the temperature sensing characteristic of the same cabbage type rape variety single plant in the step 5) is as follows:
the plant height h' of the spring sowing plant obtained by measuring according to the step 4) and the average plant height of the field autumn sowing plant obtained by calculating according to the step 3)
Calculating the relative plant height Hc of the single plants of all the cabbage type rape varieties during spring sowing in the field; judging the temperature sensing characteristic of the single plant of the cabbage type rape variety according to the relative plant height Hc of all the cabbage type rape varieties; the specific calculation mode of the relative plant height Hc of the single plants of all the cabbage type rape varieties is as follows:
wherein:
HCis the relative plant height of single plants of all cabbage type rape varieties during spring sowing in the field;
h' is the plant height of the spring sowing plant in cm;
3. The method for identifying the temperature-sensitive characteristics of Brassica napus by using the relative plant height of the plant according to claim 2, wherein: the judgment condition for judging the temperature sensing characteristic of the single plant of the cabbage type rape variety according to the relative plant height Hc of the single plant of all the cabbage type rape varieties during autumn sowing in the field is as follows: hc is 0, and the temperature sensitivity is strongest; hc is more than 0 and less than or equal to 0.2, and the temperature sensitivity is strong; hc is more than 0.2 and less than or equal to 0.4, and the temperature sensitivity is weak; hc is greater than 0.4, and the temperature sensitivity is poor.
4. The method for identifying the temperature-sensitive characteristics of Brassica napus by using the relative plant height of plants as claimed in claim 1, wherein: the specific implementation mode of judging the temperature sensing characteristics of all cabbage type rape varieties during autumn sowing in the field in the step 5) is as follows:
respectively calculating the average plant height of each cabbage type rape variety sowed in the field in spring
According to the average plant height of the same cabbage type rape variety in spring sowing in the field
And the average plant height of the variety in autumn sowing in the field
Calculating the relative plant height Hs of the variety; judging the temperature sensing characteristic of the current cabbage type rape variety according to the relative plant height Hs of the current variety;
the average plant height of the field sowed in spring
The calculation method of (1) is as follows:
wherein:
h' is the measured plant height of the spring sowing plant in cm;
n is the number of plant plants in the full-bloom period of the rape after the spring sowing of the field;
the calculation mode of the relative plant height Hs of the current variety single plant is as follows:
wherein:
hs is the relative plant height of the current variety;
5. The method for identifying the temperature-sensitive characteristics of Brassica napus according to claim 4, wherein the plant height is determined by the following steps: the judgment condition for judging the temperature sensing characteristic of the current cabbage type rape variety according to the relative plant height Hs of the current variety is as follows: hs is less than 0.2, and has the strongest temperature sensitivity and strong winterness; hs is more than or equal to 0.2 and less than 0.3, and has strong temperature sensitivity and winter property; hs is more than or equal to 0.3 and less than 0.4, and has weak temperature sensitivity and half-winterness; hs is more than 0.4, poor temperature sensitivity and springiness.
6. The method for identifying the temperature-sensitive characteristics of Brassica napus according to claim 1 or 2 or 3 or 4 or 5, wherein the relative plant height of the plants is selected from the group consisting of: the brassica napus variety to be identified is a combination of a plurality of new oil No. 23, 16TS 306-4 (spring) guining, 16TS306-3 choosen 1 guining, 16TS 312-2 guining, 16TS 309-10 guining, 16NPZ 269-1 mixed selection, 16NPZ 158 mixed selection, 15NS 45-4 mixed selection, 20168 (G) selection, 2016TS (G)10, solar oil 2266 and solar oil 2288.
7. The method for identifying the temperature-sensitive characteristics of Brassica napus according to claim 6, wherein the plant height is determined by the following steps: the number of plants of each variety of the cabbage type rape variety to be identified is not less than 30 when the cabbage type rape variety is sowed in the field in autumn.
8. The method for identifying the temperature-sensitive property of Brassica napus by using the relative plant height of the plant according to claim 7, wherein: the number of the cabbage type rape varieties to be identified is N; the total number of the plants sowed in autumn in the field is M; m is more than or equal to 30N + 10.
9. The method for identifying the temperature-sensitive property of Brassica napus by using the relative plant height of the plant according to claim 8, wherein: the condition for measuring the plant heights h of all cabbage type rape varieties to be identified after autumn sowing in the field in the step 2) is that the number of the plants opened in the full-bloom stage of the rape is not less than 2/3M; and M is the total number of plants sowed in autumn in the field.
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