CN110089368A - A method of Winter Cole-wort winter resistance is identified by measurement hypocotyl length - Google Patents
A method of Winter Cole-wort winter resistance is identified by measurement hypocotyl length Download PDFInfo
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- CN110089368A CN110089368A CN201910381474.8A CN201910381474A CN110089368A CN 110089368 A CN110089368 A CN 110089368A CN 201910381474 A CN201910381474 A CN 201910381474A CN 110089368 A CN110089368 A CN 110089368A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention relates to a kind of new methods that Winter Cole-wort winter resistance is identified by measurement hypocotyl length, mainly comprise the steps that (1) field planting；(2) hypocotyl length is measured；(3) single plant winter resistance power is carried out to determine；(4) ratio (R) that hypocotyl length strain numbers at different levels account for group is calculated；(5) according to the rank where the ratio R >=90% of the plant Shuo Zhan group of hypocotyl lengths at different levels, determine that group's winter resistance is strong and weak.The identification method overcomes the shortcomings that traditional wintering rate identification, Physiology and biochemistry identification and molecular labeling method identification winter resistance, present age detection can be achieved, experiment condition and operating technology are required low, with easy to operate, at low cost, the advantages that visual result is reliable, is suitable for Winter Cole-wort cold hardness evaluation.
Description
Technical field
The invention belongs to plant cold resistance identification technology fields, are related to a kind of side for identifying Winter Cole-wort winter resistance
Method, i.e., it is strong and weak using hypocotyl length evaluation Winter Cole-wort winter resistance.
Background technique
Rape is the important oil crops in China, wherein cabbage type rape (Brassica napus It L.) is China's growing surface
The maximum rape type of product, is divided into winter rape and spring rape according to sowing season.Winter Cole-wort has yield height, anti-fall
Volt, many merits such as quality is good, but winter resistance is poor, and winter low temperature etc. is that limitation the northern area of China Winter Cole-wort is sent out
The main environment factor of exhibition.Therefore, strong winter resistance Winter Cole-wort material selective breeding develops Winter Cole-wort to northern China
Production is of great significance, and accurate, Rapid identification its winter resistance is the key link of the strong cold-resistant Winter Cole-wort of breeding,
It is the key link that northern area selects Winter Cole-wort kind.
Problem of the prior art: the identification of Winter Cole-wort winter resistance at present mainly passes through measurement Physiology and biochemistry and field
The methods of wintering rate identification of indicator, these identification methods are time-consuming, and qualification result also changes greatly.Therefore it needs more reliable straight
It sees, conveniently cold hardness evaluation method is to improve Winter Cole-wort cold-tolerance breeding efficiency.
Summary of the invention
It is an object of the invention to solve Winter Cole-wort cold hardness evaluation, provide a kind of simple, fast
Speed, low cost, the method for efficiently and accurately identifying Winter Cole-wort winter resistance, to improve Winter Cole-wort cold-resistant material
Screening efficiency.
To achieve the above object, the technical scheme adopted by the invention is that: pass through measurement hypocotyl length identify Wild cabbage type
Winter rape winter resistance, specifically includes: the present embodiment provides one kind to identify Winter Cole-wort cold-resistant by measurement hypocotyl length
The method of property, specifically sequentially includes the following steps:
1) field planting: by Winter Cole-wort material to be identified according to normal sowing time (August 20th or so) field seeding, kind
The total strain number of plant is no less than 50 plants.
2) hypocotyl length measures: it is grown 70~80 days after emergence, measures the hypocotyl length of Varieties In The Seedling Stage to be identified, until
It is 50 plants or more few.
3) carry out single plant winter resistance power to determine: hypocotyl length (L) < 0.5cm is I grade, superpower cold-resistant；0.6cm≤under
Plumular axis length (L) < 1.0cm is II grade, Qiang Kanghan；1.1cm≤hypocotyl length (L) < 1.5cm is III grade, cold-resistant；1.6cm
≤ hypocotyl length (L) < 2.0cm is IV grade, is resisted cold；Hypocotyl length (L) >=2.1cm is IV grade, not cold-resistant.
4) ratio (R) that hypocotyl length strain numbers at different levels account for group: the corresponding winter resistance grade defined according to (3) is calculated,
Hierarchical statistics hypocotyl length strain numbers at different levels, and calculate separately the ratio (R) that hypocotyl length strain numbers at different levels account for group.
R=Nl/Nt
In formula: NlFor the strain number of hypocotyl lengths at different levels in material to be identified；NtFor material total strain number to be identified.
5) according to the rank where the ratio R >=90% of the plant Shuo Zhan group of hypocotyl lengths at different levels, group's cold-resistant is determined
Property it is strong and weak: RL<0.5>=90%, superpower cold-resistant is I grade；R0.6≤L<1.0>=90%, Qiang Kanghan are II grade；R1.1≤L<1.5>=90%, resist
It is cold, it is III grade；R1.6≤L<2.0>=90%, it resists cold, is IV grade；RL≥2.1>=90%, cold-resistant, is not V grade.
Detailed description of the invention
Fig. 1 is that single plant winter resistance power determines；
Fig. 2 is the Lanzhou the 2015-2016 town Shang Chuan test result-group's winter resistance power identification；
Fig. 3 is that group's winter resistance power determines；
Fig. 4 is 12 Winter Cole-wort semilethal low temperature (LT50) ranges；
Fig. 5 is different winter resistance Winter Cole-wort material germination period hypocotyl lengths；
Fig. 6 is different winter resistance Winter Cole-wort material seedling stage hypocotyl morphological differences；
Fig. 7 is the relationship of Winter Cole-wort hypocotyl length and semilethal low temperature.
Specific embodiment
Application method and device in the following embodiments of the invention patent are unless otherwise specified conventional method and device；
Equipment used, reagent are the conventional equipment and reagent of Reagent Company's purchase.For make the purpose of the invention patent, technical solution and
Advantage is clearer, and the specific embodiment of the invention patent is described in detail combined with specific embodiments below.These are excellent
The example of embodiment is selected to be illustrated in a particular embodiment.
Here, it should also be noted that, in order to avoid having obscured the technical side of the invention patent because of unnecessary details
Case illustrate only the technical solution and/or processing step closely related with the scheme of patent according to the present invention in embodiment,
And the little other details of relationship are omitted.
Embodiment 1
The present embodiment provides a kind of methods for identifying Winter Cole-wort winter resistance by measurement hypocotyl length, specifically by following
Step carries out:
1) field planting: by Winter Cole-wort material to be identified according to normal sowing time (August 20th or so) field seeding, kind
The total strain number of plant is no less than 50 plants；
2) hypocotyl length measures: it is grown 70~80 days after emergence, measures the hypocotyl length in material seedling stage to be identified, at least 50
Plant or more；
3) carry out single plant winter resistance power to determine: hypocotyl length (L) < 0.5cm is I grade, superpower cold-resistant；0.6cm≤hypocotyl
Length (L) < 1.0cm is II grade, Qiang Kanghan；1.1cm≤hypocotyl length (L) < 1.5cm is III grade, cold-resistant；1.6cm≤under
Plumular axis length (L) < 2.0cm is IV grade, is resisted cold；Hypocotyl length (L) >=2.1cm is IV grade, not cold-resistant.See Fig. 1 (table 1).
4) ratio (R) that hypocotyl length strain numbers at different levels account for group is calculated.The corresponding winter resistance grade defined according to (3),
Hierarchical statistics hypocotyl length strain numbers at different levels, and calculate separately the ratio (R) that hypocotyl length strain numbers at different levels account for group.
R=Nl/Nt
In formula: NlFor the strain number of hypocotyl lengths at different levels in material to be identified；NtFor material total strain number to be identified.
5) according to fig. 2 (table 2), the rank where the ratio R >=90% of the plant Shuo Zhan group of hypocotyl lengths at different levels, really
Grouping body winter resistance is strong and weak: RL<0.5>=90%, superpower cold-resistant is I grade；R0.6≤L<1.0>=90%, Qiang Kanghan are II grade；
R1.1≤L<1.5>=90%, cold-resistant is III grade；R1.6≤L<2.0>=90%, it resists cold, is IV grade；RL≥2.1>=90%, cold-resistant, is not V grade.See
Fig. 3 (table 3).
Embodiment 2
The present embodiment provides the measuring methods of hypocotyl length and Winter Cole-wort cold-resistant relevance, this experiment is in 2015-
It is tested in the Lanzhou town Shang Chuan within 2016, experimental field 38 ° of north latitude, height above sea level 2150m are specifically sequentially included the following steps:
(1) field planting: by 12 Winter Cole-wort material 309-4, TS309-3 to be identified, 312-2,309-10,269,
158,45-4,8 (G), G10), fresh oil No. 23, day oil 2266, day oil 2288 according to normal sowing time (August 20th or so) big
Field sowing, the total strain number of plantation are no less than 50 plants.
(2) semilethal low temperature measures
Cold-resistant rank determines: determine that the winter resistance of Winter Cole-wort is strong and weak according to semilethal low temperature, < -1.5 DEG C of LT50, and cold-resistant
Rank is I grades；1.0 DEG C of -1.5 DEG C≤LT50 <, cold-resistant rank are II grade；< -0.6 DEG C of -1.0 DEG C≤LT50, cold-resistant rank are
III grade；< -0.2 DEG C of -0.6 DEG C≤LT50, cold-resistant rank are IV grade；- 0.2 DEG C≤LT50, cold-resistant rank is V grade.Specific knot
Fruit sees Fig. 4 (table 4).
(3) hypocotyl length measures: after emergence and when growing 70~80 days, the hypocotyl length of material to be identified is measured,
50 plants of measurement records measurement result (Fig. 5, Fig. 6).
(4) semilethal low temperature and hypocotyl length correlation analysis
Such as Fig. 7, hypocotyl length and semilethal low temperature linear model are found, the winter resistance of Winter Cole-wort and lower embryo
Shaft length is related, and winter resistance is stronger, and hypocotyl length is shorter.Semilethal greenhouse and the correlation of hypocotyl length reach 0.96.
The principle of the present invention: after planting, hypocotyl is longer before the winter for Winter Cole-wort, and winter resistance is weaker, otherwise winter resistance
It is stronger.Mainly foundation is for this judgement: (1) soil moisture is higher than air themperature and stabilization, and hypocotyl length before the winter can make to grow
Cone bassets, and exposure causes to endure cold in air.And hypocotyl is longer, the aerial volume of growth cone exposure, area
Bigger, the probability by freeze injury is bigger.When growth cone bassets the height for reaching certain, wintering rate reduces cannot even get over completely
In the winter, hypocotyl does not extend or extends and is less before the winter, and plant crawls in ground, then growth cone cannot basset or basset
Part is few, and the aerial part of exposure is few, and winter resistance is strong, and wintering rate is high.(2) by measuring 12 known winter resistances powers
The hypocotyl length of Winter Cole-wort material, in conjunction with semilethal low temperature, it was demonstrated that hypocotyl length can be used as Winter Cole-wort
The index of cold hardness evaluation.Thus it is strong and weak that material winter resistance to be identified can be divided according to hypocotyl length before the winter, winter resistance power
5 grades: I grades can be divided into, be superpower cold-resistant, II grade is Qiang Kanghan, and III grade is cold-resistant, IV grade be it is cold-resistant, V grade is not cold-resistant.It is sweet
After blue type winter rape planted in fall, because the sensibility to photo-thermal is different, the small error that will cause, but not influence to determine winter resistance
Strong and weak reliability.
The above is only the specific embodiment of the application, it is noted that for the ordinary skill people of the art
For member, under the premise of not departing from the application principle, several improvements and modifications can also be made, these improvements and modifications are also answered
It is considered as the protection scope of the application.
Claims (7)
1. a kind of method for identifying Winter Cole-wort winter resistance by measurement hypocotyl length, which is characterized in that mainly include
Following steps: (1) field planting；(2) hypocotyl length is measured；(3) single plant winter resistance power is carried out to determine；(4) it calculates at different levels
Hypocotyl length strain number accounts for the ratio (R) of group；(5) according to ratio R >=90% of the plant Shuo Zhan group of hypocotyl lengths at different levels
The rank at place determines that group's winter resistance is strong and weak.
2. a kind of method that Winter Cole-wort winter resistance is identified by measurement hypocotyl length according to claim 1,
It is characterized in that, the field planting specifically: by Winter Cole-wort material to be identified according to normal sowing time (August 20th
Left and right) in field seeding, the total strain number of plantation is no less than 50 plants.
3. a kind of method that Winter Cole-wort winter resistance is identified by measurement hypocotyl length according to claim 1,
It is characterized in that, the hypocotyl length measurement specifically: grown 70~80 days after material emergence to be identified and start to measure lower embryo
Shaft length, at least 50 plants or more.
4. a kind of method that Winter Cole-wort winter resistance is identified by measurement hypocotyl length according to claim 1,
It is characterized in that, the single plant winter resistance power criterion are as follows: hypocotyl length (L) < 0.5cm is I grade, superpower cold-resistant；
0.6cm≤hypocotyl length (L) < 1.0cm is II grade, Qiang Kanghan；1.1cm≤hypocotyl length (L) < 1.5cm is III grade, is resisted
It is cold；1.6cm≤hypocotyl length (L) < 2.0cm is IV grade, is resisted cold；Hypocotyl length (L) >=2.1cm is IV grade, not cold-resistant.
5. a kind of method that Winter Cole-wort winter resistance is identified by measurement hypocotyl length according to claim 1,
It is characterized in that, determining that group is anti-according to the rank where the ratio R >=90% of the plant Shuo Zhan group of hypocotyl lengths at different levels
Cold power；Specifically: RL<0.5>=90%, superpower cold-resistant is I grade；R0.6≤L<1.0>=90%, Qiang Kanghan are II grade；R1.1≤L<1.5
>=90%, cold-resistant is III grade；R1.6≤L<2.0>=90%, it resists cold, is IV grade；RL≥2.1>=90%, cold-resistant, is not V grade.
6. a kind of method that Winter Cole-wort winter resistance is identified by measurement hypocotyl length according to claim 1,
It is characterized in that, the ratio (R) for calculating hypocotyl length strain numbers at different levels and accounting for group, method is, according to claim 4 institute
The corresponding winter resistance grade defined, hierarchical statistics hypocotyl length strain numbers at different levels, and calculate separately hypocotyl length strain numbers at different levels and account for
The ratio (R) of group；
R=Nl/Nt
In formula: NlFor the strain number of hypocotyl lengths at different levels in kind to be identified；NtFor kind total strain number to be identified.
7. the association analysis method of hypocotyl length and Winter Cole-wort cold-resistant, which is characterized in that specific steps are as follows: (1)
Field planting；(2) semilethal low temperature measures；(3) hypocotyl length measures；(4) semilethal low temperature and hypocotyl length correlation
Analysis；(5) single plant winter resistance power determines.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116267454A (en) * | 2023-02-02 | 2023-06-23 | 安徽农业大学 | Method for screening cold-resistant varieties of non-heading Chinese cabbages |
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| CN103773798A (en) * | 2014-01-08 | 2014-05-07 | 华中农业大学 | Application of PLD (phospholipase D) epsilon gene in increasing nitrogen nutrition effective utilization and seed yield of crops |
| CN105794479A (en) * | 2016-04-13 | 2016-07-27 | 甘肃农业大学 | Method for utilizing plant heights to identify winterness strength of Brassica campestris L. |
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| CN103773798A (en) * | 2014-01-08 | 2014-05-07 | 华中农业大学 | Application of PLD (phospholipase D) epsilon gene in increasing nitrogen nutrition effective utilization and seed yield of crops |
| CN105794479A (en) * | 2016-04-13 | 2016-07-27 | 甘肃农业大学 | Method for utilizing plant heights to identify winterness strength of Brassica campestris L. |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116267454A (en) * | 2023-02-02 | 2023-06-23 | 安徽农业大学 | Method for screening cold-resistant varieties of non-heading Chinese cabbages |
| CN116267454B (en) * | 2023-02-02 | 2023-11-24 | 安徽农业大学 | Method for screening cold-resistant varieties of non-heading Chinese cabbages |
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Application publication date: 20190806 |