CN105794479B - A method of it is strong and weak using plant height identification Chinese cabbage type winter rape winter habit - Google Patents
A method of it is strong and weak using plant height identification Chinese cabbage type winter rape winter habit Download PDFInfo
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- CN105794479B CN105794479B CN201610227970.4A CN201610227970A CN105794479B CN 105794479 B CN105794479 B CN 105794479B CN 201610227970 A CN201610227970 A CN 201610227970A CN 105794479 B CN105794479 B CN 105794479B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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Abstract
A method of strong and weak using plant height identification Chinese cabbage type winter rape winter habit, by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified in field planting, total seedling number is at 30 plants or more；It is grown 60~65 days after emergence, measures the plant height of all seedling in Chinese cabbage type winter rape variety group to be identified；Carry out plant height standardization；Standardized according to plant height and determine that single plant winter habit is strong and weak: 0.9≤Z < 1 is IV grade, spring habit；0.5≤Z < 0.9 is III grade, weak winter habit；0.2≤Z < 0.5 is II grade, winter habit；Z < 0.2 is I grade, strong winter habit.The identification method overcomes the shortcomings that statistics, Physiology and biochemistry and molecular labeling method of traditional Chinese cabbage type winter rape wintering rate, easy to operate, realizes present age detection, and it is strong and weak to identify single plant winter habit, low to laboratory condition and technical requirements, at low cost, result is reliable.
Description
Technical field
The invention belongs to plant cold resistance identification technology fields, are related to a kind of method for identifying Chinese cabbage type winter habit power, special
It is not related to a kind of method using plant height identification Chinese cabbage type winter rape winter habit power.
Background technique
Global climate warms up in recent years, and winter crop Wintering Period temperature generally improves, and the frequency that extreme low temperature occurs is reduced,
Duration is reduced.Global wanning is particularly evident in the area northern China Han Han, the Chinese cabbage type winter oil with characteristics such as high yield, precocities
The research that dish moves northward feasibility also just becomes one of hot issue of rape research and North Dry Han Qu reply global wanning is chosen
One of important technique measure and strategy of war, wherein cold-resistant breed breeding is the key that the northern winter rape production of development, and cold-resistant
The breeding of kind first has to solve winter habit power identification method simple, effectively, inexpensive.
Conventional cold hardness evaluation is using identification methods such as statistics, Physiology and biochemistry method and the molecular labelings of field wintering rate.
The statistics of field wintering rate is big by extraneous human factor, and accuracy is not high.
Physiology and biochemistry identification method needs certain equipment, drug, and the bad grasp of most of physiological and biochemical indexs, change
Change it is big, be not sufficiently stable, be time-consuming and laborious, is at high cost, accuracy it is not high.
Molecular labeling method is the molecular labeling that first find with kind winter habit power close linkage, then with the molecular labeling
It is detected, is determined according to the presence or absence of key band.This method advantage is that testing result is more accurate, and contemporary inspection may be implemented
It surveys.The disadvantage is that requiring strictly the operation of molecular labeling, exploitation label is at high cost, at present only in a small number of unit applications.
Summary of the invention
The object of the present invention is to provide it is a kind of using plant height identification Chinese cabbage type winter rape winter habit power method, accuracy compared with
Height, and convenient for operation.
To achieve the above object, the technical scheme adopted by the invention is that: it is a kind of using plant height identify Chinese cabbage type winter rape
The method of winter habit power, specifically sequentially includes the following steps:
1) by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified in field planting, total seedling number of plantation is at 30 plants or more；
2) it is grown 60~65 days after emerging, measures the plant height of all seedling in Chinese cabbage type winter rape variety group to be identified；
3) according to the plant height of all seedling measured, plant height standardization is carried out:
Z=X/Xmax
In formula: Z is plant height standardization；X is the plant height of the seedling measured, unit mm；XmaxFor plant height highest in group,
Unit mm；
4) standardized according to plant height and determine that single plant winter habit is strong and weak: 0.9≤Z < 1 is IV grade, spring habit；0.5≤Z < 0.9 is
III grade, weak winter habit；0.2≤Z < 0.5 is II grade, winter habit；Z < 0.2 is I grade, strong winter habit.
Identification method of the present invention calculates plant height standardization by the plant height of measurement Chinese cabbage type winter rape group to differentiate wait reflect
The shortcomings that ding white ware dish type winter rape variety winter habit is strong and weak, overcomes statistics, Physiology and biochemistry and the molecular labeling method of traditional wintering rate,
It is easy to operate, it realizes present age detection, identifies single plant winter habit power, at low cost, result low to laboratory condition and technical requirements can
It leans on.
Specific embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
The present invention is specifically sequentially included the following steps: using the method for plant height identification Chinese cabbage type winter rape winter habit power
1) field planting: by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified in field planting, since strain number is very little, meeting
The reliability of result is influenced, so total seedling number of plantation is at 30 plants or more；
2) survey of growth and development: growing 60~65 days after emergence, measures institute in Chinese cabbage type winter rape variety group to be identified
There is the plant height of seedling；
3) it according to the plant height (X) of all seedling measured, carries out plant height standardization (Z), i.e., with the strain of each seedling in group
High (X) is respectively divided by highest plant height (X in groupmax):
Z=X/Xmax
4) single plant winter habit power determines: it is IV grade that 0.9≤plant height, which standardizes (Z) < 1, spring habit；The standardization of 0.5≤plant height
(Z) < 0.9 is III grade, weak winter habit；It is II grade that 0.2≤plant height, which standardizes (Z) < 0.5, winter habit；Plant height standardizes (Z) < 0.2
I grade, strong winter habit.
For winter rape after spring sowing, plant height is higher, and winter habit is weaker, otherwise winter habit is stronger.Mainly foundation is the spring for this judgement
Change effect: if plant passes through vernalization, winter rape ability bolting completely, normal growth reaches certain plant height；If plant vernalization is complete
Entirely, winter rape energy bolting, but it is unable to normal growth, plant height cannot reach normal height；If plant cannot pass through vernalization, winter oil
Dish is unable to bolting, is constantly in nutrient growth, crawls in ground.Thus winter rape winter habit power can be divided into 4 grades: I grades, be
Strong winter habit, II grade is weak winter habit, and III grade is winter habit, and IV grade is spring habit.
5) group's winter habit power determines: determining group according to the ratio (B) that the strain number that plant height standardizes (Z) < 0.2 accounts for group
Body winter habit is strong and weak, 85.00%≤B, I grade of winter habit power rank, is superpower winter variety；75%≤B < 85%, winter habit power rank
II grade, be strong winter variety；65%≤B < 75%, III grade of winter habit power rank are winter variety；20%≤B < 65%, winter habit is strong
IV grade of weak rank is weak winter variety；B < 20%, V grade of winter habit power rank are spring varieties.
Winter rape is after spring sowing, according to determining that tested variety winter habit is strong and weak in experimental plot by vernalization strain number completely,
Main foundation, under same environmental condition, winter habit kind of different strengths and weaknesses is significant by vernalization degree difference.Natural vernalization
Under the conditions of carry out winter habit divide have certain reasonability.
Winter rape is after spring sowing, and because of the difference of winter habit power, different cultivars difference in plant height is significant, small caused by upper in measurement
Error will not influence the reliability for determining winter rape winter habit power.
Rape winter habit power is related with vernalization characteristic, and the different genotypical sensibility to temperature of winter resistance have differences,
Winter resistance is stronger, and winter habit is stronger, and vernalization required temperature is lower, the time is longer；Under same growing environment, winter resistance power is not
Same kind, can be by vernalization, but vernalization degree is inconsistent, causes the plant height of group uneven, (former according to this phenomenon
Reason), the difference of kind winter habit can be both identified, the winter habit that can also identify single plant is strong and weak.
Embodiment: the town Shang Chuan of Lanzhou in 2014 test
(1) field planting: by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified in field planting, total seedling number 30 plants with
On；
(2) survey of growth and development: growing 60~65 days after emergence, measures institute in Chinese cabbage type winter rape variety group to be identified
There is plant height；
(3) plant height standardization (Z): according to all plant heights in Chinese cabbage type winter rape variety group to be identified are measured, strain is carried out
High standardization (Z), i.e., with all plant heights (X) in group respectively divided by highest plant height (Xmax) in group, it may be assumed that (Z=X/Xmax)
4) single plant winter habit power determines: it is IV grade that 0.9≤plant height, which standardizes (Z) < 1, spring habit；The standardization of 0.5≤plant height
(Z) < 0.9 is III grade, weak winter habit；It is II grade that 0.2≤plant height, which standardizes (Z) < 0.5, winter habit；Plant height standardizes (Z) < 0.2
I grade, strong winter habit.
5) group's winter habit power determines: determining group according to the ratio (B) that the strain number that plant height standardizes (Z) < 0.2 accounts for group
Body winter habit is strong and weak, 85.00%≤B, I grade of winter habit power rank, is superpower winter variety；75%≤B < 85%, winter habit power rank
II grade, be strong winter variety；65%≤B < 75%, III grade of winter habit power rank are winter variety；20%≤B < 65%, winter habit is strong
IV grade of weak rank is weak winter variety；B < 20%, V grade of winter habit power rank are spring varieties.
By winter rape variety to be identified at the beginning of the last month of summer late spring field seeding, grown 60~65 days after emergence, measure it is to be identified
Chinese cabbage type winter rape variety group plant height calculates plant height standard, determines Chinese cabbage type winter rape variety winter habit power rank to be identified.
It the results are shown in Table 1.
1 2014 years Lanzhou of the table town Shang Chuan test result
。
Claims (1)
1. a kind of method using plant height identification Chinese cabbage type winter rape winter habit power, specifically sequentially includes the following steps:
1) by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified in field planting, total seedling number of plantation is at 30 plants or more；If
Chinese cabbage type winter rape variety to be identified be it is two or more, then total seedling number of each Chinese cabbage type winter rape variety to be identified plantation is equal
At 30 plants or more；
2) it is grown 60~65 days after emerging, measures all seedling in the group that each kind Chinese cabbage type winter rape to be identified is formed
Plant height；
3) according to the plant height of all seedling measured, plant height standardization is carried out:
Z=X/Xmax
In formula: Z is plant height standardization；X is the plant height of the seedling measured, unit mm；XmaxFor a particular species Chinese cabbage to be identified
Highest plant height in the group that type winter rape is formed, unit mm；
4) standardized according to plant height and determine that single plant winter habit is strong and weak: 0.9≤Z < 1 is IV grade, spring habit；0.5≤Z < 0.9 is III grade,
Weak winter habit；0.2≤Z < 0.5 is II grade, winter habit；Z < 0.2 is I grade, strong winter habit；
5) group's winter habit power determines: the ratio-dependent group winter habit for accounting for group according to the strain number that plant height standardizes < 0.2 is strong and weak,
The ratio indicates that then 85.00%≤B, I grade of winter habit power rank, are superpower winter variety with B；75%≤B < 85%, winter habit are strong and weak
II grade of rank, be strong winter variety；65%≤B < 75%, III grade of winter habit power rank are winter variety；20%≤B < 65%, winter
Property IV grade of rank of power be weak winter variety；B < 20%, V grade of winter habit power rank are spring varieties.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107896979B (en) * | 2017-11-16 | 2019-11-22 | 甘肃农业大学 | The selection of the excellent plant type of winter habit Chinese cabbage type winter rape |
| CN108812110B (en) * | 2018-04-28 | 2020-08-04 | 甘肃农业大学 | Method for identifying temperature sensing characteristics of brassica napus by using relative plant height of plants |
| CN110089368A (en) * | 2019-05-08 | 2019-08-06 | 甘肃农业大学 | A method of Winter Cole-wort winter resistance is identified by measurement hypocotyl length |
| CN110810173B (en) * | 2019-10-29 | 2021-05-18 | 甘肃农业大学 | Method for identifying cold resistance of cabbage type winter rape by root system characters |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686148A (en) * | 2015-02-25 | 2015-06-10 | 滨州市作物研究所 | Method for identifying the springness and winterness of wheat varieties and regulating flower synchronization |
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| CN104686148A (en) * | 2015-02-25 | 2015-06-10 | 滨州市作物研究所 | Method for identifying the springness and winterness of wheat varieties and regulating flower synchronization |
Non-Patent Citations (4)
| Title |
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| 小麦冬春性鉴定研究;张灿军;《中国优秀硕士论文全文数据库》;20101115(第11期);全文 |
| 甘蓝冬性鉴定方法及标准的研究;李长缨 等;《华北农学报》;19991231;第14卷(第3期);123-127 |
| 白菜型冬油菜抗寒机理与选择指标体系研究;杨宁宁;《中国优秀硕士学位论文全文数据库》;20150515(第5期);全文 |
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Inventor after: Sun Wancang Inventor after: Guo Rendi Inventor after: Wang Jijun Inventor after: Pu Yuanyuan Inventor after: Ma Chi Inventor after: Dong Xiaoyun Inventor after: Bai Jing Inventor after: He Huili Inventor after: Hou Xianfei Inventor after: Li Xuecai Inventor after: Gu Yuanguo Inventor after: Liu Zigang Inventor after: Zeng Xiucun Inventor after: Wu Junyan Inventor after: Fang Yan Inventor after: Yang Gang Inventor before: Sun Wancang Inventor before: Hou Xianfei Inventor before: Li Xuecai Inventor before: Liu Zigang Inventor before: Zeng Xiucun Inventor before: Wu Junyan Inventor before: Fang Yan Inventor before: Yang Gang Inventor before: Guo Rendi |
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