CN111855586B - Method for rapidly screening pineapple variety cold resistance - Google Patents
Method for rapidly screening pineapple variety cold resistance Download PDFInfo
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- CN111855586B CN111855586B CN202010498793.XA CN202010498793A CN111855586B CN 111855586 B CN111855586 B CN 111855586B CN 202010498793 A CN202010498793 A CN 202010498793A CN 111855586 B CN111855586 B CN 111855586B
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Abstract
The invention discloses a method for rapidly judging the cold resistance of pineapple varieties. According to the method, the pineapple varieties with strong cold resistance can be quickly and effectively screened out through cold treatment, the screening material is simple, planting is not needed, the detection cost is reduced, and the detection steps are simplified; in addition, in the whole screening process, destructive effect on samples is avoided, physiological indexes are not required to be measured, the whole process is simple and easy to master, the result is reliable, the screening process of pineapple cold-resistant variety resources is shortened, the cold-resistant breeding process of pineapples is accelerated, and the cold-resistant breeding period of pineapples is shortened.
Description
Technical Field
The invention relates to the field of plants, in particular to a method for rapidly screening the cold resistance of pineapple varieties.
Background
Pineapple is one of famous tropical fruits, and has a large area for planting in provinces such as Guangdong, Guangxi, Hainan, Fujian, Yunnan, Taiwan and the like.
The temperature is decisive for the growth and development of the pineapples, and the low temperature in winter is a main factor limiting the planting range of the pineapples. In winter every year, pineapples in places such as Guangdong, Guangxi and Fujian in China are often damaged by low-temperature cold tides in the north, so that the growth of pineapple plants and fruits is influenced. The breeding and wide popularization of the new variety of the cold-resistant pineapples is an important measure for improving the cold resistance of the pineapples and reducing the loss of the pineapple cold damage. The pineapple variety has rich resources and various cold resistance, the cold resistance of different pineapple varieties is screened, and the pineapple variety resource with better cold resistance is screened, so that the method is the basis for breeding new varieties of cold-resistant pineapples.
In the prior art, the cold resistance of pineapple variety resources is mainly determined by culturing pineapple potted seedlings, performing cold treatment in an artificial climate chamber, and determining the changes of indexes such as conductivity, MDA, antioxidant enzyme activity, soluble sugar and soluble protein. However, the existing identification technology has the disadvantages of long detection period, complicated technical measures and high test cost. There is a need for a method for rapidly determining and discriminating the cold tolerance of different pineapple varieties.
Disclosure of Invention
One of the purposes of the invention is to provide a method for rapidly judging the cold resistance of pineapple varieties;
the invention also aims to provide a cold-resistant pineapple variety screening system;
the invention also aims to provide application of the cold-resistant pineapple variety screening system in screening cold-resistant pineapple varieties.
The technical scheme adopted by the invention is as follows:
in a first aspect of the present invention, there is provided:
a method for rapidly screening the cold resistance of pineapple varieties comprises the following steps:
picking up pineapple suckling sprouts, and measuring colorimetric values L, a and b of the upper parts of leaves of the suckling sprouts;
cold treating the bud sucking seedling;
observing the color change of the suckling seedling leaves after the cold treatment, and measuring colorimetric values L, a and b of the suckling seedling leaves after the cold treatment at the upper parts;
judging the cold resistance of the pineapple variety according to the change degree of the colorimetric values L, a and b;
wherein the colorimetric value L is a color brightness value;
the colorimetric value a is a red color value;
the abovementioned color values b are color yellow values.
Wherein the cold treatment time is 70-72 hours.
Specifically, the pineapple variety has the following cold resistance: the leaves of the suckling seedlings do not turn red or yellow after cold treatment, and the pineapple varieties have stronger cold resistance when the colorimetric values L, a and b change less after the cold treatment. Specifically, during the cold treatment, the pineapple bud-sucking seedlings do not need soil planting.
Specifically, the cold treatment conditions are as follows: the temperature is 4-6 deg.C and the relative humidity is 65-75%.
Specifically, the length of the pineapple suckling sprout is 30-40 cm.
Specifically, in the screening method, the minimum sample quantity of the suckling sprouts of each pineapple variety is greater than or equal to 5.
In a second aspect of the present invention, there is provided:
a cold-resistant pineapple variety screening system comprises:
a chromaticity detection device;
a cold treatment device;
an analysis device.
Wherein, above-mentioned colourity detection device includes the color recognition device, can discern or survey the color of pineapple bud seedling leaf.
The cold treatment device comprises an incubator and other equipment with a temperature constant function.
Specifically, the analysis device analyzes and analyzes the color of the leaves of the bud seedling after cold treatment and the change degree of colorimetric values L, a and b, and judges the cold resistance of the pineapple variety.
More specifically, the pineapple variety is judged to have cold resistance as follows: the leaves of the suckling seedlings do not turn red or yellow after cold treatment, and the pineapple varieties have stronger cold resistance when the colorimetric values L, a and b change less after the cold treatment.
In a third aspect of the present invention, there is provided:
the screening system is applied to screening of cold-resistant pineapple varieties.
The invention has the beneficial effects that:
1. the screening material only needs fresh bud-sucking seedlings of the pineapples, and does not need to be planted, so that the detection cost is reduced, and the detection steps are simplified;
2. the screening method does not need destructive sampling, does not need to measure physiological indexes, and only needs to measure chromatic values; the whole process is simple to operate, easy to master and reliable in result, the resource screening process of the pineapple cold-resistant variety is shortened, the cold-resistant breeding process of the pineapple is accelerated, and the cold-resistant breeding period of the pineapple is shortened.
Drawings
FIG. 1 shows fresh suckling seedlings of pineapple after cold treatment, wherein A is an cold-resistant variety and B is a cold-resistant variety.
Detailed Description
In order to make the objects, technical solutions and technical effects of the present invention more clear, the present invention will be described in further detail with reference to specific embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
The experimental materials and reagents used are, unless otherwise specified, all consumables and reagents which are conventionally available from commercial sources.
Example 1
The test is designed with 2 treatments, namely, pineapple bud-sucking seedlings (the length is about 35 cm and the color is consistent) of 2 different varieties (a variety A which is not cold-resistant and a variety B which is cold-resistant) are put into a thermostat with the temperature of 5 ℃ and the relative humidity of 70 percent for low-temperature treatment. This experiment was repeated 3 times, with 2 seedlings being repeated a single time, i.e. 6 seedlings per treatment.
After 72 hours of low-temperature treatment, the bud-sucking seedlings are moved out of the incubator. The colorimetric values (initial colorimetric value a and cold-treated colorimetric value b) of each seedling were measured. The middle upper part (i.e., middle and tip) of the leaf was measured for colorimetric measurements, and 3 leaves were measured per seedling.
TABLE 1 color change of leaves after low-temperature treatment of different varieties
Wherein, L is the brightness value of the color of the leaves, a is the red value of the color, and b is the yellow value of the color.
As can be seen from table 1, the color brightness value (L) of the leaves of the cold-intolerant variety a after low-temperature treatment is significantly lower than that of the cold-tolerant variety B; the color red value (a) is obviously higher than that of the cold-resistant variety B; the color yellow value (B) is slightly lower than that of the cold-resistant variety B.
After the cold-intolerant variety A is treated at low temperature, the leaves become red obviously, and the color red value (a) is high; the cold-resistant variety B is still dark green after being treated at low temperature, and has high green value (a is negative).
Example 2
The method of the invention is used for screening the cold resistance of 5 different pineapple varieties, and 2 cold-resistant varieties and 3 cold-resistant varieties are screened out. The 5 pineapple varieties were subjected to cold tolerance verification. And randomly selecting 100 seedling of each of 5 pineapple varieties, and planting the seedling in northern Guangdong mountainous areas with lower air temperature in winter. After the low temperature in winter, after the temperature rises again, the overwintering condition and the growth condition of the pineapples are investigated, the investigation is carried out from the last 12 months to the last 3 months of the next year, and the investigation mode is to count the overwintering survival rate and the number of newly-extracted leaves of the pineapples. And (5) verifying the cold resistance in actual planting.
Table 2 actual cold-resistant effect of 5 pineapple varieties screened by the method
From the test results in table 2, it can be found that the cold-resistant varieties screened by the method have high accuracy, can effectively distinguish the cold resistance and the cold intolerance of different pineapple varieties, and have extremely high agricultural application value.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (4)
1. A method for rapidly screening the cold resistance of pineapple varieties is characterized by comprising the following steps:
picking up pineapple suckling sprouts with the length of 30-40cm, and measuring colorimetric values L, a and b of the upper middle parts of leaves of the suckling sprouts;
cold treating the bud seedling at 4-6 deg.C and relative humidity of 65-75%;
observing the color change of the suckling seedling leaves after the cold treatment, and measuring colorimetric values L, a and b of the suckling seedling leaves after the cold treatment at the upper parts;
judging the cold resistance of the pineapple variety according to the change degree of the colorimetric values L, a and b, wherein the leaves of the bud sucking seedling after cold treatment do not turn red or yellow, and the smaller the change of the colorimetric values L, a and b after cold treatment is, the stronger the cold resistance of the pineapple variety is;
wherein, the colorimetric value L is a color brightness value;
the colorimetric value a is a red color value;
the colorimetric value b is a color yellow value;
during the cold treatment, the pineapple bud-sucking seedlings do not need soil planting.
2. The screening method according to claim 1, wherein the minimum number of sprouted seedlings in each pineapple variety is 5 or more.
3. The cold-tolerant pineapple variety screening system based on the rapid screening method of claim 1 or 2, wherein the system comprises:
a chromaticity detection device;
a cold treatment device;
an analysis device;
the analysis device judges the cold resistance of the pineapple variety by analyzing the color of the leaves of the suckling seedlings after cold treatment and the change degree of the colorimetric values L, a and b, the leaves of the suckling seedlings after cold treatment do not turn red or yellow, and the smaller the change of the colorimetric values L, a and b after cold treatment is, the stronger the cold resistance of the pineapple variety is.
4. Use of the screening system of claim 3 in screening cold resistant pineapple varieties.
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