CN110100593B - Method for identifying cold resistance of tea tree variety - Google Patents
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Abstract
The invention discloses a method for identifying cold resistance of a tea tree variety, which comprises the following steps: cutting young shoots on a crown of a tea tree to be identified in cold tolerance and uniformly dividing the young shoots into 2 groups, freezing the young shoots of the tea tree in a test group, and respectively carrying out the following treatments on the young shoots of the tea tree in a control group and the young shoots of the tea tree in the test group after freezing: inserting the basal part of the young tea tree sprout into water according to the insertion depth of 4-6 cm and placing the young tea tree sprout at room temperature, taking the 2 nd leaf to the 4 th leaf under the terminal bud of the tail part of the young tea tree sprout, measuring the color difference value a, calculating the anti-freezing index X, and obtaining the freezing injury index: the lower the freezing injury index, the stronger the cold tolerance of the variety. The cold resistance of the tea tree is identified by freezing young shoots of the tea tree and comparing the change of the chromatic aberration value of the leaves of the tea tree before and after freezing; has the technical advantages of correct, quick and convenient detection result.
Description
Technical Field
The invention relates to the field of cold tolerance identification of plants in winter, in particular to a method for predicting cold tolerance of tea trees in winter by freezing young shoots of tea trees by a refrigerator and then measuring the color difference value change of tea tree leaves before and after freezing.
Background
The tea tree originates from tropical rainforests in southwest areas of China and has the characteristic of being pleased with warm and cold for the growth environment. Therefore, the freeze injury caused by low-temperature stress in winter in tea tree introduction areas is a common natural disaster in tea tree cultivation, can cause serious inhibition of tea tree growth, can also cause damage and even death of tea trees in serious cases, and brings serious loss to tea production. However, during the long-term propagation and evolution process, tea plants are subjected to different winter low-temperature environmental stresses due to different distribution areas, and the varieties are differentiated strongly and weakly, such as the critical low temperature of the tolerance of different oolong varieties is-9 ℃ to-12 ℃, and the semi-lethal temperature (LT50) of green tea varieties is 6.84 ℃ to-11.52 ℃. The breeding of cold-resistant tea tree varieties is an important factor for reducing low-temperature stress and freeze injury in winter and realizing high and stable yield. Cold tolerance identification is an important means for breeding cold tolerance varieties, and the existing method for identifying the cold tolerance of tea trees comprises the following steps: firstly, a field natural identification method is adopted, namely field investigation is carried out during the overwintering period of the tea trees, particularly after low-temperature freeze injury occurs, and the drought tolerance of the tea trees is evaluated according to the proportion of the leaves of the tea trees which are frozen; the 'conductivity measuring method' is that the 2 nd to 3 rd leaves below the upper bud of the mature young sprout of the tea tree are taken during the overwintering period, freezing treatment at different temperatures is carried out, the conductivity of the boiled tea is taken as reference, the relative conductivity w% is calculated to be (freezing conductivity/boiling conductivity) multiplied by 100, and the temperature when the relative conductivity is 50% is determined to be the critical low temperature which can be endured by the variety to be measured; thirdly, identifying the low-temperature freezing rate of the cutting seedlings, namely, putting the cutting seedlings of the tea trees into refrigerators with different temperatures for freezing for 3 hours, then cutting off a power supply to naturally cool the cutting seedlings to room temperature, observing the freezing condition of tea tree leaves every 2-3 days until the freezing symptom is stable (generally, continuously observing for about 10 days), and taking the freezing symptom of 30 percent of leaves as the critical low temperature which can be endured by the variety; and fourthly, a blade anatomical structure evaluation method, namely taking mature blades of different varieties to carry out microscopic section observation and measurement, and calculating the thickness ratio (grid/sea ratio) of the fence tissue of the blade to the sponge tissue, wherein the larger the ratio is, the stronger the cold resistance is. The cold tolerance identification methods of the tea trees have the defects that the field natural identification method is completely limited by the temperature in winter, and the identification cannot be carried out in winter with higher temperature. The 'conductivity measurement method' and 'low-temperature freezing rate identification' of the cutting seedlings require setting refrigerators with different low temperatures for series temperature treatment, and long-time complicated observation is required after the treatment to obtain experimental data. The 'leaf anatomical structure evaluation method' is a brief introduction evaluation method, and the critical low temperature which is endured by the variety cannot be directly evaluated by the height of the grid/sea ratio. The method for developing the method for rapidly, accurately and intuitively identifying the cold tolerance of the tea tree variety is an urgent need of tea tree breeding.
Disclosure of Invention
The invention aims to solve the technical problem of providing a novel method for identifying the cold resistance of tea trees.
In order to solve the technical problems, the invention provides a method for identifying the cold resistance of a tea variety, which comprises the following steps:
1) shearing 10-16 branches of young shoots on a crown of a tea tree to be identified in cold tolerance and uniformly dividing the young shoots into 2 groups (5-8 branches in each group), wherein one group is a test group, and the other group is a control group;
the number of the sheared young shoots is even; the young shoot refers to a branch with more than or equal to 5 leaves (mature leaves) on the tea tree;
note: the tea tree refers to mature tea tree, namely tea tree with age more than or equal to 4 years and capable of collecting tea;
the young shoots are shoots satisfying the following conditions: when the branches are cut in 6-12 months, "young shoots" refer to branches which are annual and have more than 5 mature leaves on the tea trees; when the branches are cut in 1-5 months, the 'young shoots' refer to mature branches which grow in the previous year and have more than 5 mature leaves; the cut young shoots have 5-8 mature leaves, and the length is not more than 30cm or the number of the leaves is not more than 8;
generally, the following subsequent steps are carried out within 1-2 hours after picking, namely, the young shoots of the tea trees in the test group are subjected to the freezing treatment in the step 2), and the young shoots of the tea trees in the control group are subjected to the treatment in the step 3);
2) freezing the young shoots of the tea trees in the test group at (-15 +/-1) DEG C for 1-2 hours;
3) and respectively carrying out the following treatment on the young tea tree shoots of the control group and the young tea tree shoots of the frozen test group obtained in the step 2):
inserting the base part of the young tea tree shoot into water according to the insertion depth of 4-6 cm, placing the young tea tree shoot in the water at room temperature (20-25 ℃) for 2 +/-0.2 hours, taking the 2 nd leaf to the 4 th leaf below the terminal bud of the young tea tree shoot, measuring the color difference value a, and respectively obtaining the average value (a) of the color difference values a of the leaves in the test groupTesting) Average value of color difference a of control group leaf (a)Control)；
Note: the end of a fracture formed when the young tea tree sprout is cut is called the base of the young tea tree sprout, and the other end of the young tea tree sprout is called the tail; the 2 nd to 4 th leaves below the terminal bud at the tail part of the new shoot mean the 2 nd to 4 th leaves counted from the tail part of the new shoot;
4) calculating the freezing resistance index Xa:
freezing resistance index Xa ═ aTesting/aControl；
5) Calculating the freezing injury index:
substituting the freezing resistance index Xa as an independent variable (Xa) into a linear regression equation Y of 50.789-47.875 Xa; y is the freezing injury index of the tea tree variety to be identified;
6) and judging:
the lower the freezing injury index is, the stronger the cold resistance of the variety is;
or selecting at least 2 tea tree varieties as the to-be-detected products, and performing young tea tree shoots of the to-be-detected products according to the steps 1) -5), wherein the to-be-detected variety with the lowest Y value is the tea tree variety with the strongest cold tolerance in the to-be-detected products.
The improvement of the identification method of the cold tolerance of the tea tree variety of the invention is as follows:
in the step 2), the freezing treatment temperature is-15 ℃ and the time is 2 hours.
The method for identifying the cold tolerance of the tea tree variety is further improved as follows:
in the step 3), inserting the base part of the young tea tree shoot into water according to the insertion depth of 5-6 cm;
and taking the 3 rd leaf below the terminal bud of the young sprout, and determining the color difference value a.
The invention comprises the following steps:
as a reference for evaluating the cold tolerance of the tea tree variety, the invention provides an identification result of a field natural identification method for the tested tea tree variety at the end of 2 months in 2018 (the lowest field temperature is-5 ℃ in 29 months in 1 month in 2018), and constructs a tea tree variety cold injury index prediction model.
Firstly, the construction of a prediction model of the invention is carried out according to the following steps in sequence:
1. and (3) field frost resistance index determination of different tea tree varieties: the minimum temperature of the planting plots of the tested varieties is-5 ℃ in 2018, 1 month and 29 days, the investigation is carried out in 2018, 2 months and 26-27 days, 15 tea trees are investigated randomly for each variety, the number of the frozen leaves and the number of the healthy leaves on the plants are counted respectively, and the freezing injury index H% [ Sigma (ni × xi)/N/4 ]. times.100 is calculated according to the result, wherein N is the number of the investigated plants of each variety, namely 15 plants; ni is the number of frozen plants at each level; the number of the frozen stages of each plant is (0 stage: frozen leaves account for less than 5% of the total number of the leaves; 1 stage: 5% < frozen leaves account for less than 15% of the total number of the leaves; 2 stage: 15% < frozen leaves account for less than 25% of the total number of the leaves; 3 stage: 25% < frozen leaves account for less than 50% of the total number of the leaves; 4 stage: frozen leaves account for more than 50% of the total number of the leaves); 4 is the highest number of grades of freezing injury (grade 0-4). The method is used for investigating 47 varieties, wherein the freezing injury indexes H of 45 varieties are used for constructing a linear prediction model (table 1), and 2 varieties are used for verifying the model (table 2).
The number of frozen stages is judged according to 'identification method of nature in field' (Royi, Donglijuan, Duanhua, Lisaijun, Fuding Dabai tea, etc.) 20 kinds of tea plant seedlings are identified and evaluated for cold resistance, tea communication, 2013,40 (2): 3-5).
TABLE 1 Freeze resistance index and freeze injury index determined for different tea plant varieties
1: freezing injury index H [ ∑ (n) ]i×xi)/N/4]X 100, where N is the number of investigated plants per variety, i.e. 15; n isiThe number of frozen plants at each level; the number of frozen stages of each plant (0 stage: the total number of frozen leaves<5 percent; level 1: 5 percent of<The frozen leaves account for the total number of the leaves<15 percent; and 2, stage: 15 percent of<The frozen leaves account for the total number of the leaves<25 percent; and 3, level: 25 percent of<The frozen leaves account for the total number of the leaves<50 percent; 4, level: the frozen leaves account for the total number of the leaves>50％)。
2: frost resistance index XLMean of color difference L values after 6 shoots freezing/mean of color difference L values before 6 shoots freezing.
3: and the freezing resistance index Xa is the average of the values of the chromatic aberration a of the blades after 6 fresh tips are frozen and/or the average of the values of the chromatic aberration a of the blades before 6 fresh tips are frozen.
4: the frost resistance index Xb is the average of the b values of the color difference of the leaves after 6 fresh tips are frozen/the average of the b values of the color difference of the leaves before 6 fresh tips are frozen.
TABLE 2 Freeze injury index and freeze resistance index of tea varieties ' 2-49 ' and ' 5-28
*: the index calculation method is the same as in table 1.
2. Comparative study of freezing conditions: taking 3 varieties of Zhe nong 113, Ying Shuang Bei and Ying Shuang, namely mature young tea trees with the age of more than one bud and six leaves, and dividing 24 branches of each variety into 6 branches. One group is used as a control, the base part of the group is inserted in 5-6cm of clear water and is placed at room temperature (20-25 ℃); and freezing the other 3 groups in a refrigerator with the temperature controlled at-5 deg.C, -15 deg.C and-25 deg.C for 2 hours, taking out, inserting the young sprout base part with the length of 5-6cm into clear water, standing at room temperature for 4 hours, and measuring the third color difference value (L, a, b) under the terminal bud by using a color difference meter at 1, 2 and 4 hours. As a result, the tea leaves treated at-5 ℃ are basically kept green within the observation period of 4 hours, and have no significant difference from the tea leaves which are not subjected to freezing treatment; the tea leaves treated at the temperature of minus 25 ℃ are all in pork liver color, and the color difference among varieties is not obvious; only the tea leaves treated at the temperature of-15 ℃ have obvious difference in color difference between varieties during the standing for 1-2 hours (figure 1), and the color difference values of all varieties are very close to each other when the tea leaves are placed for 4 hours and the color difference values of all varieties are dark red. According to the results of this experiment, it was confirmed that the optimum treatment conditions were freezing treatment at-15 ℃ for 2 hours, then the base was inserted in water and left to stand at room temperature (20-25 ℃) for 2 hours, and the difference in color value was measured.
The mature tea tree refers to a tea tree with the age of more than or equal to 4 years and capable of collecting tea; the young shoots are shoots satisfying the following conditions: when the branches are cut in 6-12 months, "young shoots" refer to branches which are annual and have more than 5 mature leaves on the tea trees; when the branches are cut in 1-5 months, the 'young shoots' refer to mature branches which grow in the previous year and have more than 5 mature leaves; the cut young shoots have 5-8 mature leaves and the length is not more than 30cm or the number of leaves is not more than 8.
Note: the value of the color difference parameter a is related to the green and red degrees of the tea, when a is a negative value, green is presented, and the larger the negative number is (the smaller the absolute value is), the darker the green is; when the value a is positive, the tea leaves are red, and the larger the absolute value of the value a is, the more red the color is. After freezing, the colors of the leaves are different due to different cold resistances, and the color change of the tea of the variety with strong cold resistance is small (the green is slightly reduced, and the red degree is slightly improved);
the freezing resistance index Xa is used to predict the freezing damage index H.
3. And (3) measuring the freezing resistance index: taking the tea tree varieties in the tables 1-2, wherein the tea tree varieties have mature young shoots with more than 5 leaves, each variety has 12 branches and is divided into 2 groups, each group has 6 branches, one group is a control group, and the other group is a test group. Inserting the young sprout base of the control group with a distance of 5-6cm into clear water, and standing at room temperature; and (3) freezing the young shoots of the test group in a refrigerator at the temperature of-15 ℃ for 2 hours according to the method for comparing and researching the freezing treatment conditions in the step (2), taking out the young shoots, inserting the young shoots into a clean water greenhouse for placing for 2 hours, and then measuring the color difference values (L, a and b) of the leaves of the control group and the test group. The freezing resistance index is calculated according to the following formula: frost resistance index XLThe mean of the frozen leaf color difference L values/mean of the unfrozen leaf color difference L values; the freezing resistance index Xa is the average value of the chromatic aberration a of the frozen blade/the average value of the chromatic aberration a of the unfrozen blade; and the frost resistance index Xb is the average of the values of the frozen blade color difference b/the average of the values of the unfrozen blade color difference b. The results are shown in tables 1 and 2.
4. Constructing a tea tree variety freeze injury index prediction model: first, the freezing injury index H in Table 1 is calculated to be respectively equal to the freezing resistance index XLThe results are given in Table 3, along with the linear correlation coefficients between the frost resistance Xa and Xb indices. The freeze injury index H and the freezing resistance index Xa have an extremely obvious negative correlation, and the freeze injury index H and the freezing resistance index XLAnd an anti-freeze index XbThere is no significant correlation between them. At a freezing resistance indexXa is independent variable (Xa), and the freezing injury index H obtained by field investigation is dependent variable (Y) to establish a linear regression equation: y50.789-47.875 Xa (p)<0.001) that can be used to predict or identify cold tolerance of tea varieties.
TABLE 3 coefficient of linear correlation between freezing injury index H and freezing resistance index
| Parameter(s) | Freezing injury index H |
| Frost resistance index XL | -0.025 |
| Freezing resistance index Xa | -0.807* |
| Frost resistance index Xb | -0.203 |
*:p<0.01.
Secondly, the cold tolerance identification method of the tea tree variety is sequentially carried out according to the following steps:
1. cutting 12 mature young shoots with more than 5 leaves from a variety tree of tea trees to be tested, dividing the mature young shoots into 2 groups, wherein each group comprises 6 branches, one group is a control group, and the other group is a test group; inserting the 5-6cm base of the young sprout of the control group into a beaker or a water tray filled with clear water, and standing at room temperature; and (3) putting the young shoots of the test group into a freezing box with the temperature set to be-15 ℃ for freezing for 2 hours, taking out the young shoots, inserting the young shoots into a beaker or a water pan filled with clear water for 5-6cm, and standing for 2 hours at room temperature.
2. Measuring the color difference value a of the third leaf below the top bud of the new shoot of the test group and the control group by using a color difference meter, and dividingRespectively calculating the average a of the color difference values a of the leaves of the control group and the test groupControlAnd aTestingThe frost resistance factor Xa is calculated according to the following formula: freezing resistance index Xa ═ aTesting/aControl。
3. And substituting the freezing resistance index Xa of the variety to be detected into the freezing damage index model Y of 50.789-47.875Xa by taking the freezing resistance index Xa of the variety to be detected as an independent variable (Xa), wherein the calculated Y value is the predicted value of the freezing damage index H of the variety to be detected. The smaller the predicted value of the freezing injury index H is, the lighter the freezing injury degree of the variety is, and the stronger the cold resistance is; the larger the index, the heavier the degree of freezing damage of the variety and the worse the cold resistance. If a contrast variety is set in the test, the cold tolerance of the variety to be tested can be known to be stronger or weaker than that of the contrast variety by comparing the Y values of the variety to be tested and the contrast variety.
In conclusion, the invention develops a novel method for identifying the cold resistance of the tea trees, namely, the fresh tips of the tea trees are frozen by using a refrigerator, and then the color difference values of the leaves of the tea trees before and after freezing are compared to identify the cold resistance of the tea trees. Has the technical advantages of correct, quick and convenient detection result.
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The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
FIG. 1 is a comparison of color difference values a of different varieties of leaves with time.
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto:
example 1, a method for identifying cold tolerance of a tea variety:
1. cutting 12 young shoots of mature leaves with 5 leaves from 6-year-old tea trees of tea tree varieties '2-49' and '5-28', dividing into 2 groups, wherein each group comprises 6 branches, one group is a control group, and the other group is a test group; inserting the 5-6cm of the base of the young sprout of the control group into a beaker or a water tray filled with clear water, and standing for 2 hours at room temperature (20-25 ℃); and (3) putting the young shoots of the test group into a freezing box with the temperature set to be-15 ℃ for freezing for 2 hours, taking out the young shoots, inserting the young shoots into a beaker or a water tray filled with clear water by 5-6cm at the base parts of the young shoots (namely, the young shoots are inserted into the water by 5-6cm), and standing for 2 hours at room temperature.
2. Measuring the color difference value a of the third leaf under the terminal bud of the new shoot tail of the test group and the control group by using a color difference meter, and respectively calculating the average value a of the color difference values of the leaves of the control group and the test groupTestingAnd aControlAccording to the formula: the freezing resistance index Xa is the average color difference value a of the leaves of the test groupTestingControl leaf group average color difference value aControl. The results are shown in table 2, the antifreeze index Xa of tea variety '2-49' is 0.549, and the antifreeze index Xa of variety '5-28' is 0.723.
3. The freezing injury index of the variety '2-49' is 24.51 and the freezing injury index of the variety '5-28' is 16.18 by taking the freezing resistance index Xa of the tea variety '2-49' which is 0.549 and the freezing resistance index Xa of the variety '5-28' which is 0.723 as independent variables Xa and substituting the freezing injury index formula Y which is 50.789-47.875 Xa.
Actually measured number of freezing injury plants in field investigation: ` cultivars 2-49 ` were 25.00 and cultivars ` 5-28 ` were 18.33 (Table 2). The identification value of the invention is very close to the actual field measurement value, and the same trend that the degree of freezing injury of the variety '2-49' is higher than that of the variety '5-28' is also presented, which indicates that the cold resistance of the variety '5-28' is stronger than that of the variety '2-49'.
Example 2, a method for identifying cold tolerance of a tea variety:
in the process of breeding tea plant varieties, a plurality of varieties to be identified and reference varieties are generally planted on a plot with the same fertility level, then field freezing condition investigation is carried out after the winter low-temperature freezing period, the damage condition between the varieties to be identified and the reference varieties is compared, or the cold resistance strength of the reference varieties is sequenced, so that the cold resistance information of the varieties to be identified is obtained for reference in future variety popularization or introduction. However, cold tolerance identification cannot be performed if no low temperature freezing climate occurs during the test. In this case, the technique of the present invention can be applied for identification.
In the embodiment, the 'Fuding white tea' (the semilethal temperature LT) with the semilethal temperature determined by identification is selected50-4.50 ℃), ' morning ' of mulukhi ' (LT)50-4.08 ℃) and ' north' (LT)503 varieties such as-2.01 ℃) are materials [ Huanghaitao, Yuanzhong, Zhanwei, Zhousheng, Aocheng, screening research of cold-resistant tea tree varieties, Chinese agronomy report, 2012,28(25):219 and 223.]These 3 varieties were used as samples. 12 mature shoots were cut from each variety, and the rest was identical to example 1.
The results are shown in Table 4, i.e., the cold resistance is ranked as: 'Fuding white tea' (the predicted freezing injury index H is 25.04)>'Wuniuzao' (predicted freezing injury index H ═ 33.25)>' North species' (predicted freezing injury index H ═ 35.58), with published semilethal temperature LT50The ordering is completely consistent.
Table 4. Cold tolerance identification results of 3 tea plant varieties in example 2
Comparative example 1-1, the "-15 ℃ freezing 2 hours" in example 2 was changed to "-5 ℃ freezing 2 hours", and the rest was the same as in example 2.
Comparative example 1-2, the "-15 ℃ freezing 2 hours" in example 2 was changed to "-20 ℃ freezing 2 hours", and the rest was the same as in example 2.
Comparative example 2-1, the "taking out and inserting the young sprout base 5-6cm into a beaker or a water pan filled with clear water, and standing at room temperature" in example 2 was changed from 2 hours to 1 hour; the rest is equivalent to example 2.
Comparative example 2-2, the "taking out and inserting the young sprout base 5-6cm into a beaker or a water pan filled with clear water, and standing at room temperature" in example 2 was changed from 2 hours to 3 hours; the rest is equivalent to example 2.
Comparative example 3-1, the "depth of young sprout inserted into water of 5-6 cm" in example 2 was changed to "depth of young sprout inserted into water of 2-3 cm"; the rest is equivalent to example 2.
Comparative example 3-2, the "depth of the young sprout into water of 5-6 cm" in example 2 was changed to "insert the whole young sprout into water"; the rest is equivalent to example 2.
The resulting freeze injury index Y of the above comparative example is compared to that of example 2, as set forth in Table 2 below.
TABLE 2 Freeze injury index Y predicted by different treatments
| Fuding white tea | Wuniuzao | North variety | |
| Example 2 | 25.04 | 33.25 | 35.58 |
| Comparative examples 1 to 1 | 5.70 | 4.84 | 7.71 |
| Comparative examples 1 to 2 | 42.19 | 42.38 | 43.42 |
| Comparative example 2-1 | 12.85 | 13.59 | 13.45 |
| |
32.71 | 35.47 | 34.55 |
| Comparative example 3-1 | 24.61 | 33.31 | 33.81 |
| Comparative examples 3 to 2 | 38.24 | 40.22 | 40.29 |
The above comparative example has a drawback that the judgment result is incorrect or effective judgment cannot be made.
Finally, it is also noted that the above-mentioned lists merely illustrate a few specific embodiments of the invention. It is obvious that the invention is not limited to the above embodiments, but that many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.
Claims (3)
1. The method for identifying the cold tolerance of the tea tree variety is characterized by comprising the following steps of:
1) cutting 10-16 branches of young shoots on a crown of a tea tree to be identified in cold tolerance and uniformly dividing the branches into 2 groups, wherein one group is a test group, and the other group is a control group;
2) freezing the young shoots of the tea trees in the test group at (-15 +/-1) DEG C for 1-2 hours;
3) and respectively carrying out the following treatment on the young tea tree shoots of the control group and the young tea tree shoots of the frozen test group obtained in the step 2):
the base part of the young sprout of the tea tree is inserted into water according to the insertion depth of 4-6 cm and placed at room temperature (2 +/-0.2) for a whileAfter the test, taking the 2 nd leaf to the 4 th leaf under the terminal bud of the tail part of the young shoot, measuring the color difference value a, and respectively obtaining the average value (a) of the color difference value a of the leaves in the test groupTesting) Average value of color difference a of control group leaf (a)Control)；
4) Calculating the freezing resistance index Xa:
freezing resistance index Xa ═ aTesting/aControl；
5) Calculating the freezing injury index:
substituting the freezing resistance index Xa as an independent variable (Xa) into a linear regression equation Y of 50.789-47.875 Xa; y is the freezing injury index of the tea tree variety to be identified;
6) and judging:
the lower the freezing injury index is, the stronger the cold resistance of the variety is;
or selecting at least 2 tea tree varieties as the to-be-detected products, and performing young tea tree shoots of the to-be-detected products according to the steps 1) -5), wherein the to-be-detected variety with the lowest Y value is the tea tree variety with the strongest cold tolerance in the to-be-detected products.
2. The method for identifying the cold tolerance of a tea variety as claimed in claim 1, wherein the method comprises the following steps:
in the step 2), the freezing treatment temperature is-15 ℃ and the time is 2 hours.
3. The method for identifying cold tolerance of a tea variety according to claim 1 or 2, wherein:
in the step 3), inserting the base part of the young tea tree shoot into water according to the insertion depth of 5-6 cm;
and taking the 3 rd leaf below the terminal bud of the young sprout, and determining the color difference value a.
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| CN112986311B (en) * | 2021-01-29 | 2023-09-01 | 商丘市农林科学院 | Method for identifying low-temperature resistance of wheat variety in spring |
| CN113029975A (en) * | 2021-04-06 | 2021-06-25 | 中国计量大学 | Method for identifying quality of freeze injury tea |
| CN116267454B (en) * | 2023-02-02 | 2023-11-24 | 安徽农业大学 | Method for screening cold-resistant varieties of non-heading Chinese cabbages |
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