CN110100593A - The identification method of tea tree breed cold-hartliness - Google Patents
The identification method of tea tree breed cold-hartliness Download PDFInfo
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- CN110100593A CN110100593A CN201910387495.0A CN201910387495A CN110100593A CN 110100593 A CN110100593 A CN 110100593A CN 201910387495 A CN201910387495 A CN 201910387495A CN 110100593 A CN110100593 A CN 110100593A
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Abstract
The invention discloses a kind of identification methods of tea tree breed cold-hartliness, the following steps are included: the young sprout on clip cold-hartliness tea tree tree crown to be identified is divided into 2 groups, test group And Development of Tea Shoot elder generation freezing processing, test group And Development of Tea Shoot after control group And Development of Tea Shoot and freezing is handled as follows respectively: the base portion of And Development of Tea Shoot is inserted into water according to the insertion depth of 4~6cm and is placed at room temperature, take the 2nd leaf~the 4th leaf under the terminal bud of young sprout tail portion, measure value of chromatism a, calculate freeze proof index X, carry out acquisition freezing damage index: freezing damage index is lower, the cold-hartliness of kind is stronger.Then the present invention compares the tea leaf value of chromatism variation of freezing front and back, identifies tea tree cold-hartliness using freezing And Development of Tea Shoot；, fast, easily technical advantage correct with testing result.
Description
Technical field
The present invention relates to the fields of plant winter cold-hartliness identification, concretely relate to a kind of utilization refrigerator freezing tea tree
Young sprout, then measurement freezing front and back tea leaf value of chromatism variation, the method to predict tea tree winter field cold-hartliness.
Background technique
Tea tree originates from Southwestern China area tropical rain forest, the characteristic for having happiness temperature cold growing environment.Therefore tea tree
Introducing a fine variety regional winter low temperature stress causes freeze injury to be natural calamity common in tree plant cultivation, it can cause growth of tea plant by
It is serious to inhibit, it also will cause tea tree injury even death when serious, bring serious loss to Tea Production.However, tea tree exists
In long-term propagation and evolutionary process, it is subjected to different winter low temperature environment-stress because of Distribution Area difference, different cultivars
Between produce the differentiation of cold-hartliness power, the low critical temperature of such as different oolong varieties tolerance is -9 DEG C~-12 DEG C, and green tea
The semilethal low temperature (LT50) of kind is 6.84 DEG C~-11.52 DEG C.The cold-resistant tea tree breed of breeding is to reduce winter low temperature stress to freeze
Evil realizes an important factor for stable high yield.Cold-hartliness identification is breeding cold-resistant variety important means, existing tea tree cold-hartliness mirror
Fixed method has: 1. " Natural infection method " especially carries out field after low temperature freezing-disaster generation that is, in tea tree overwintering period
Investigation is evaluated tea tree Drought hardiness by the ratio of freeze injury according to tea leaf；2. " conductimetric method " takes tea in overwintering period
2nd~3 leaf under bud on the mature young sprout of tree, carries out the freezing processing of different temperatures, and to be boiled the conductivity of rear tealeaves as ginseng
According to, relative conductivity w%=(freezing conductivity/boil conductivity) × 1 00 is calculated, when relative conductivity being 50%
Temperature is set to the low critical temperature that tested kind is resistant to；3. tea cutting seedling, i.e., be put by " cuttage seeding low temperature endure cold rate identification "
The refrigerator freezing of different temperatures 3 hours, then cutting off the power allows its cooled to room temperature, later primary every observation in 2~3 days
Tea leaf is endured cold situation, (is generally observed continuously 10 days or so) until freeze injury symptom is stablized, is gone out with 30% blade
The low critical temperature that existing freeze injury symptom is resistant to as the kind；" 4. natomical leaf structure evaluation assessment ", that is, take different cultivars at
Ripe blade carries out microsection Observe and measure, calculates blade palisade tissue and spongy tissue thickness ratio (grid/sea ratio), the ratio
Bigger, cold-hartliness is stronger.These tea tree cold-hartliness identification method all Shortcomings, such as " Natural infection method " are limited by completely
Winter temperature cannot then carry out identification in temperature higher winter.And " conductimetric method " and " cuttage seeding low temperature endure cold rate mirror
The refrigerator for then needing to set Different hypothermia calmly " carries out series of temperatures processing, and many and diverse observation of long period is also needed after processing
It can obtain experimental data." natomical leaf structure evaluation assessment " is a kind of brief introduction evaluation assessment, and grid/sea, which compares so as to see who is superior, cannot directly evaluate product
The low critical temperature of kind tolerance.The method for developing quick, accurate and intuitive identification tea tree breed cold-hartliness is that Tea Breeding compels to be essential
It asks.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of tea tree cold-hartliness new Identification Method, the present invention utilizes freezing tea
Young sprout is set, then compares the tea leaf value of chromatism variation of freezing front and back, identifies tea tree cold-hartliness.
In order to solve the above technical problem, the present invention provides a kind of identification method of tea tree breed cold-hartliness, including it is following
Step:
1) it, the young sprout 10~16 on clip cold-hartliness tea tree tree crown to be identified and is divided into 2 groups (every group 5~8),
Wherein one group is test group, and another group is control group；
The quantity of the young sprout of the clip is even number；Young sprout refers to the branch on tea tree with >=5 blades (mature leaf)；
Note: tea tree refers into age tea tree, as the age of tree >=4 year, the tea tree of tealeaves can be harvested；
Young sprout is the treetop for meeting the following conditions: in clip branch in December in June-, " young sprout " refer on tea tree it is raw for the year and
Branch with 5 or more mature leafs；In 1-5 month clip branch, " young sprout " refer to the previous year growth, have 5 or more
Mature leaf maturation branch；The young sprout of clip has 5-8 mature leafs and of length no more than 30cm or blade quantity do not surpass
Cross 8 leaves；
Following subsequent step is carried out in 1-2 hour generally after picking, that is, test group And Development of Tea Shoot carries out step 2)
Freezing processing, control group And Development of Tea Shoot carry out step 3) described in processing；
2), by test group And Development of Tea Shoot prior to (- 15 ± 1) DEG C freezing processing 1~2 hour；
3), test group And Development of Tea Shoot after control group And Development of Tea Shoot and the resulting freezing of step 2) is located as follows respectively
Reason:
The base portion of And Development of Tea Shoot is transferred according in the insertion depth insertion water of 4~6cm and in (20 DEG C~25 DEG C) of room temperature
After setting (2 ± 0.2) hour, the 2nd leaf~the 4th leaf under the terminal bud of young sprout tail portion is taken, measures value of chromatism a, obtains test group blade respectively
Value of chromatism a average value (aTest), control group blade value of chromatism a average value (aControl)；
Note: forming the base portion that fracture end is known as And Development of Tea Shoot when clip And Development of Tea Shoot, the other end of And Development of Tea Shoot is then known as
Tail portion；2nd leaf~the 4th leaf under the terminal bud of young sprout tail portion refers to the 2nd leaf~the 4th leaf of the counting from young sprout tail portion；
4) freeze proof index X, is calculated:
Freeze proof index Xa=aTest/aControl；
5) freezing damage index, is calculated:
Equation of linear regression Y=50.789-47.875Xa is substituted into using freeze proof index Xa as independent variable (Xa)；Y is to be identified
The freezing damage index of tea tree breed；
6), determine:
Freezing damage index is lower, and the cold-hartliness of kind is stronger；
Alternatively, selecting at least two tea tree breed as product to be tested, by the And Development of Tea Shoot of product to be tested according to above-mentioned steps 1)~
Step 5) carries out, and the minimum kind to be measured of Y value is the strongest tea tree breed of cold-hartliness in the product to be tested.
The improvement of identification method as tea tree breed cold-hartliness of the invention:
In the step 2), freezing processing temperature is -15 DEG C, and the time is 2 hours.
The further improvement of identification method as tea tree breed cold-hartliness of the invention:
In the step 3), the base portion of And Development of Tea Shoot is inserted into water according to the insertion depth of 5~6cm；
The 3rd leaf under the terminal bud of young sprout tail portion is taken, value of chromatism a is measured.
Invention process of the invention is as follows:
As the reference of tea tree breed cold-hartliness of the present invention evaluation, provide in by the end of February, 2018 to for trying tea tree breed " field
Between natural appraisal method " qualification result (2018 field minimum temperature on January 29, be -5 DEG C), construct tea tree breed freeze injury and refer to
Number prediction model.
One, prediction model of the invention building, successively follows the steps below:
1. the freeze proof assessment of indices in Different Tea Varieties field: plantation plot on January 29th, 2018 of experimental cultivar occurs most
- 5 DEG C of low temperature, this investigation was carried out in 26-27 days 2 months 2018, and 15 plants of tea trees of each kind random searching count plant respectively
On endure cold the number of blade and healthy leaves number, freezing damage index H%=[∑ (ni × xi)/N/4] × 100 is calculated according to result, wherein N
For each Varietal Investigation plant number, i.e., 15 plants；Ni is strain numbers at different levels of enduring cold；Enduring cold for each strain, (0 grade: the blade Zhan that endures cold is total for series
The number of blade < 5%；1 grade: 5% <blade of enduring cold accounts for total leaf number < 15%；2 grades: 15% <blade of enduring cold accounts for total leaf number < 25%；3
Grade: 25% <blade of enduring cold accounts for total leaf number < 50%；4 grades: blade of enduring cold accounts for total leaf number > 50%)；4 refer to that freeze injury is classified (0-
4 grades) highest level number.For investigating 47 kinds, wherein 45 kind freezing damage index H are for constructing linear prediction model (table
1), 2 kinds are used for the verifying (table 2) of model.
It endures cold the judgement of series, is determined (Luo Yi, Dong Lijuan, Duan Jihua, Li Sai according to " Natural infection method "
20 tea tree breed seedling cold hardness evaluations such as monarch's Fuding white tea and evaluation tealeaves communication, 2013,40 (2): 3-5).
The freeze proof index and freezing damage index that table 1. measures Different Tea Varieties
1: freezing damage index H%=[∑ (ni×xi)/N/4] × 100, wherein N be each Varietal Investigation plant number, i.e., 15 plants；
niFor strain numbers at different levels of enduring cold；Enduring cold for each strain, (0 grade: blade of enduring cold accounts for total leaf number < 5% to series；1 grade: 5% <blade of enduring cold accounts for
Total leaf number < 15%；2 grades: 15% <blade of enduring cold accounts for total leaf number < 25%；3 grades: 25% <blade of enduring cold account for total leaf number <
50%；4 grades: blade of enduring cold accounts for total leaf number > 50%).
2: freeze proof index XL=6 young sprout freezing processing rear blade color difference L value average/6 young sprout freezing processing frontal lobes
Piece color difference L value average.
3: Xa=6 young sprout freezing processing rear blade color difference a value average/6 young sprout freezing processing frontal lobe of freeze proof index
Piece color difference a value average.
4: Xb=6 young sprout freezing processing rear blade color difference b value average/6 young sprout freezing processing frontal lobe of freeze proof index
Piece color difference b value average.
2. tea tree breed of table ' 2-49 ' and ' 5-28 ' freezing damage index and freeze proof index *
*: index calculation method is the same as table 1.
2. freezing processing condition comparative studies: taking ' 113 ' north kind, ‘ Shu of Zhejiang agriculture ' and ' meeting frost ' 3 tea tree breeds at age tea
The mature young sprout of above-mentioned six leaf of a bud or more is set, each kind 24 is divided into 6 one group.Wherein control is used as one group, by its base portion
5-6cm is inserted in clear water, is placed under the conditions of room temperature (20-25 DEG C)；Other 3 groups are put into temperature control at -5 DEG C, -15 DEG C respectively
After handling 2 hours with -25 DEG C of refrigerator freezings, young sprout base portion 5-6cm is inserted in clear water by taking-up, is placed 4 hours under room temperature,
Period measured third leaf color difference (L, a, b) under terminal bud with colour difference meter respectively at the 1st, 2 and 4 hour.As a result, it has been found that at 4 hours
Observation period in, by -5 DEG C handle tealeaves keep substantially green, be not significantly different with without freezing processing tealeaves；And
The tealeaves handled at -25 DEG C is all rendered as liver-coloured, and color difference is also without notable difference between kind；Only handled at -15 DEG C
Tealeaves, color difference is there are more apparent difference (Fig. 1) between kind during placing 1-2 hour, and all kinds after placement 4 hours
Leaf color is all presented kermesinus, value of chromatism also very close to.According to this experimental result, assert that optimal treatment condition is at -15 DEG C of freezings
Base portion, is then inserted in water under the conditions of (20-25 DEG C) at room temperature and places 2 hours by reason 2 hours, measures value of chromatism.
The tea tree for referring to the age of tree >=4 year at age tea tree, tealeaves being harvested；Young sprout is the treetop for meeting the following conditions: 6
When clip branch in the moon-December, " young sprout " refers to branch raw for the year and with 5 or more mature leafs on tea tree；In 1-5 month clip
When branch, " young sprout " refer to the previous year growth, have 5 or more mature leaf maturation branches；The young sprout of clip have 5-8 at
Ripe blade and of length no more than 30cm or blade quantity are no more than 8 leaves.
Note: the size of Chromaticity parameters a value is related with the green of tealeaves and red degree, and when a is negative value, green is presented, and
And negative is bigger (absolute value is smaller), green is deeper；When a value is positive number, red is presented in tealeaves, and a is worth absolute value bigger, face
Color is redder.After freezing, then because difference occur in cold-hartliness difference, leaf color, the strong kind tealeaves color change of cold-hartliness is smaller
(green slightly reduces, and red degree is slightly promoted)；
Freeze proof index Xa is for predicting freezing damage index H.
3. freeze proof assessment of indices: taking the tea tree breed in table 1~2 that there is the mature young sprout of 5 blades or more, each kind
12, it is divided into 2 groups, every group 6, wherein one group is control group, another group is test group.Control group young sprout base portion 5-6cm is inserted in
In clear water, place under room temperature；Test group is new by test group according to the method for 2 freezing processing condition comparative studies of above-mentioned steps
The tip is handled 2 hours in -15 DEG C of refrigerator freezings, is taken out and base portion is inserted in clear water medium temperature chamber is placed 2 hours, then measure control group and
Test group blade value of chromatism (L, a, b).Freeze proof index: freeze proof index X is calculated according to following formulaL=freezing blade color difference L value
Average/do not freeze blade color difference L value average；Freeze proof index Xa=freezing blade color difference a value average/do not freeze blade color
Poor a value average；Freeze proof index Xb=freezing blade color difference b value average/do not freeze blade color difference b value average.Measurement knot
Fruit sees Tables 1 and 2.
4. tea tree breed freezing damage index prediction model constructs: freezing damage index H is respectively at freeze proof index first in computational chart 1
XL, linearly dependent coefficient between freeze proof index Xa and freeze proof index Xb, as a result such as table 3.Freezing damage index H and freeze proof index Xa it
Between there are extremely significant negative correlativing relation, and freezing damage index H and freeze proof index XLWith freeze proof index XbBetween without significant correlation.With
Freeze proof index Xa is that the freezing damage index H that independent variable (Xa), field investigation obtain is that dependent variable (Y) is established equation of linear regression and obtained: Y
=50.789-47.875Xa (p < 0.001), the model can be used for predicting or identifying the cold-hartliness of tea tree breed.
Linearly related property coefficient between 3. freezing damage index H of table and freeze proof index
| Parameter | Freezing damage index H |
| Freeze proof index XL | -0.025 |
| Freeze proof index Xa | -0.807* |
| Freeze proof index Xb | -0.203 |
*:p<0.01.
Two, tea tree breed cold-hartliness identification method, successively follows the steps below:
1. there are 5 mature young sprout 12 with blade from the upper clip of tea tree breed tree to be measured, it is divided into 2 groups, every group 6
Branch, one group is control group, and one group is test group；Control group young sprout base portion 5-6cm is inserted in the beaker or water pond for filling clear water,
It is placed in room temperature condition；Test group young sprout is put into temperature to be set as freezing 2 hours in -15 DEG C of household freezer, is taken out young sprout
Base portion 5-6cm is inserted in the beaker or water pond for filling clear water, places 2 hours in room temperature condition.
2. measuring the value of chromatism a of third blade below test group and control group young sprout terminal bud with colour difference meter, calculate separately
The average a of control group and test group blade value of chromatism a outControlAnd aTest, freeze proof index Xa: freeze proof finger is calculated according to the following formula
Number Xa=aTest/aControl。
3. substituting into above-mentioned freezing damage index model Y=50.789- using the freeze proof index Xa of kind to be measured as independent variable (Xa)
47.875Xa, the Y value calculated in this way are exactly the freezing damage index H predicted value of kind to be measured.Freezing damage index H predicted value is got over
Small, the freeze injury degree of kind is lighter, and cold-hartliness is stronger；The index is bigger, and the freeze injury degree of kind is heavier, and cold-hartliness is poorer.Such as
It is equipped with check variety in fruit test, by comparing the size of kind to be measured and check variety Y value, so that it may know kind to be measured
Cold-hartliness is to be better than or be weaker than check variety.
In conclusion the present invention develops a kind of tea tree cold-hartliness new Identification Method, that is, refrigerator freezing And Development of Tea Shoot is utilized,
Then compare the tea leaf value of chromatism variation of freezing front and back, identify tea tree cold-hartliness.Correctly, quickly, conveniently with testing result
Technical advantage.
Detailed description of the invention
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing.
Fig. 1 is the comparison that different cultivars blade value of chromatism a is changed over time.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This:
Embodiment 1, a kind of identification method of tea tree breed cold-hartliness:
1. there are 5 or more mature leafs from 6 years generation age tea trees of tea tree breed ' 2-49 ' and ' 5-28 ' difference clip
Young sprout 12, be divided into 2 groups, every group 6, one group is control group, and one group is test group；Control group young sprout base portion 5-6cm is inserted
In the beaker or water pond for filling clear water, placed 2 hours at (20 DEG C~25 DEG C) of room temperature condition；Test group young sprout is put into temperature
Be set as freezing 2 hours in -15 DEG C of household freezer, then take out young sprout base portion 5-6cm is inserted in the beaker for filling clear water or
Water pond (that is, the depth in young sprout insertion water is 5-6cm), is placed 2 hours in room temperature condition.
2. measuring the value of chromatism a of third blade under test group and control group young sprout tail portion terminal bud with colour difference meter, count respectively
Calculate the average a of control group and test group blade value of chromatismTestAnd aControl, according to formula: freeze proof index Xa=test group blade
Average color difference values aTest/ control leaf group blade average color difference values aControl.Calculated result is as shown in table 2, and tea tree breed ' 2-49 ' resists
Freeze index Xa=0.549, the freeze proof index Xa=0.723 of kind ' 5-28 '.
3. being certainly with the freeze proof index Xa=0.723 of the freeze proof index Xa=0.549 of tea tree breed ' 2-49 ' and ' 5-28 '
Variable X a substitutes into freezing damage index formula Y=50.789-47.875Xa, and the freezing damage index for obtaining kind ' 2-49 ' is 24.51, product
The freezing damage index of kind ' 5-28 ' is 16.18.
The actual measurement freeze injury strain number of field investigation: ' kind 2-49 ' is 25.00, and kind ' 5-28 ' is 18.33 (tables 2).This hair
Bright identification value and field actual measured value is very close, and the freeze injury degree that kind ' 2-49 ' is presented is higher than kind ' 5-
28 ' same trend illustrates that the cold-hartliness of kind ' 5-28 ' is better than kind ' 2-49 '.
Embodiment 2, a kind of identification method of tea tree breed cold-hartliness:
In tea tree breed Breeding Process, several kinds to be identified and Reference cultivars are usually planted in same fertility water
On flat plot, field is then carried out after the overwintering low temperature freezing-disaster phase and is endured cold condition survey, kind more to be identified and reference
Victimization state between kind, or tested variety cold-hartliness power is ranked up, to obtain the cold-resistant of kind to be identified
Force information is referred to for variety popularization from now on or when introducing a fine variety.However, if there is no the appearance of low temperature freezing-disaster weather during test,
Cold-hartliness identification can not just carry out.In such a case, it is possible to be identified using the technology of the present invention.
The present embodiment has selected to compare ' the Fuding white tea ' (semilethal low temperature for specifying semilethal low temperature by identification
LT50=-4.50 DEG C), ' black ox early ' (LT50=-4.08 DEG C) and ' number north kind ' (LT50=-2.01 DEG C) etc. 3 kinds be material
[Huanghai Sea great waves, Yu Jizhong, Zhang Wei, Zhou Tiefeng, screening study China's agronomy notification of Ao Cun cold-resistant tea tree breed, 2012,28
(25): 219-223.] this 3 kinds are as sample.Each kind clip 12 mature young sprouts, remaining is equal to embodiment 1.
Acquired results are as described in Table 4, that is, cold-hartliness power sequence are as follows: " Fuding white tea " (freezing damage index H=of prediction
25.04) > ' black ox early ' (freezing damage index H=33.25)>‘ Shu north kind ' (the freezing damage index H=35.58 of prediction) of prediction, and
Semilethal low temperature LT through delivering50It sorts completely the same.
3 tea tree breed cold-hartliness qualification results in 4. embodiment 2 of table
Comparative example 1-1, " -15 DEG C freeze 2 hours " in embodiment 2 is changed to " -5 DEG C freeze 2 hours ", remaining is equal to
Embodiment 2.
Comparative example 1-2, " -15 DEG C freeze 2 hours " in embodiment 2 is changed to " -20 DEG C freeze 2 hours ", remaining is equivalent
In embodiment 2.
Comparative example 2-1, by " taking out for young sprout base portion 5-6cm to be inserted in and filling the beaker or water of clear water in embodiment 2
Disk, in the time that room temperature condition is placed " was changed to 1 hour by 2 hours；Remaining is equal to embodiment 2.
Comparative example 2-2, by " taking out for young sprout base portion 5-6cm to be inserted in and filling the beaker or water of clear water in embodiment 2
Disk, in the time that room temperature condition is placed " was changed to 3 hours by 2 hours；Remaining is equal to embodiment 2.
Comparative example 3-1, " depth in young sprout insertion water is 5-6cm " in embodiment 2 is changed to " in young sprout insertion water
Depth is 2-3cm "；Remaining is equal to embodiment 2.
Comparative example 3-2, " depth in young sprout insertion water is 5-6cm " in embodiment 2 is changed to and " is inserted into entire young sprout
In water "；Remaining is equal to embodiment 2.
The comparison of above-mentioned comparative example finally resulting freezing damage index Y and embodiment 2, are described in table 2 below.
The freezing damage index Y that table 2, different disposal are predicted
| Fuding white tea | Black ox is early | The north kind Shu | |
| Embodiment 2 | 25.04 | 33.25 | 35.58 |
| Comparative example 1-1 | 5.70 | 4.84 | 7.71 |
| Comparative example 1-2 | 42.19 | 42.38 | 43.42 |
| Comparative example 2-1 | 12.85 | 13.59 | 13.45 |
| Comparative example 2-2 | 32.71 | 35.47 | 34.55 |
| Comparative example 3-1 | 24.61 | 33.31 | 33.81 |
| Comparative example 3-2 | 38.24 | 40.22 | 40.29 |
Above-mentioned comparative example haves the defects that determine that result is incorrect or can not effectively be determined.
The above list is only a few specific embodiments of the present invention for finally, it should also be noted that.Obviously, this hair
Bright to be not limited to above embodiments, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (3)
1. the identification method of tea tree breed cold-hartliness, it is characterized in that the following steps are included:
1) 2 groups are divided into, the young sprout 10~16 on clip cold-hartliness tea tree tree crown to be identified and, wherein one group is test
Group, another group is control group；
2), by test group And Development of Tea Shoot prior to (- 15 ± 1) DEG C freezing processing 1~2 hour；
3), test group And Development of Tea Shoot after control group And Development of Tea Shoot and the resulting freezing of step 2) is handled as follows respectively:
After the base portion of And Development of Tea Shoot is inserted into water according to the insertion depth of 4~6cm and places (2 ± 0.2) hour at room temperature,
The 2nd leaf~the 4th leaf under the terminal bud of young sprout tail portion is taken, value of chromatism a is measured, obtains test group blade value of chromatism a average value respectively
(aTest), control group blade value of chromatism a average value (aControl)；
4) freeze proof index X, is calculated:
Freeze proof index Xa=aTest/aControl；
5) freezing damage index, is calculated:
Equation of linear regression Y=50.789-47.875Xa is substituted into using freeze proof index Xa as independent variable (Xa)；Y is tea tree to be identified
The freezing damage index of kind；
6), determine:
Freezing damage index is lower, and the cold-hartliness of kind is stronger；
Alternatively, selecting at least two tea tree breed as product to be tested, by the And Development of Tea Shoot of product to be tested according to above-mentioned steps 1)~step
5) it carries out, the minimum kind to be measured of Y value is the strongest tea tree breed of cold-hartliness in the product to be tested.
2. the identification method of tea tree breed cold-hartliness according to claim 1, it is characterized in that:
In the step 2), freezing processing temperature is -15 DEG C, and the time is 2 hours.
3. the identification method of tea tree breed cold-hartliness according to claim 1 or 2, it is characterized in that:
In the step 3), the base portion of And Development of Tea Shoot is inserted into water according to the insertion depth of 5~6cm；
The 3rd leaf under the terminal bud of young sprout tail portion is taken, value of chromatism a is measured.
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| CN112986311A (en) * | 2021-01-29 | 2021-06-18 | 商丘市农林科学院 | Method for identifying spring low-temperature resistance of wheat variety |
| CN113029975A (en) * | 2021-04-06 | 2021-06-25 | 中国计量大学 | Method for identifying quality of freeze injury tea |
| CN116267454A (en) * | 2023-02-02 | 2023-06-23 | 安徽农业大学 | Method for screening cold-resistant varieties of non-heading Chinese cabbages |
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