CN105794479A - Method for utilizing plant heights to identify winterness strength of Brassica campestris L. - Google Patents
Method for utilizing plant heights to identify winterness strength of Brassica campestris L. Download PDFInfo
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- CN105794479A CN105794479A CN201610227970.4A CN201610227970A CN105794479A CN 105794479 A CN105794479 A CN 105794479A CN 201610227970 A CN201610227970 A CN 201610227970A CN 105794479 A CN105794479 A CN 105794479A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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Abstract
A method for utilizing the plant heights to identify the winterness strength of Brassica campestris L.comprises the steps that the Brassica campestris L.variety to be identified is planted in land in late spring and early summer, and the total number of seedlings is more than 30; when emerged seedlings grow for 60-65 days, the plant heights of all young seedlings of the Brassica campestris L.variety to be identified are measured; the plant heights are standardized; the winterness of a single seedling is judged according to the plant height standard: the grade IV is 0.9 <= Z < 1 and has springness, the grade III is 0.5 <= Z < 0.9 and has weak winterness, the grade II is 0.2 <= Z < 0.5 and has winterness, and the grade I is Z < 0.2 and has strong winterness.The identifying method overcomes the shortcomings of traditional Brassica campestris L.wintering rate statistics and a physiological, biochemical and molecular marker method, is simple in operation, achieves current detection and identification of the winterness strength of single plants and has the low requirements for laboratory conditions and technologies, the costs are low, and results are reliable.
Description
Technical field
The invention belongs to plant cold resistance identification technology field, relate to a kind of method identifying that Chinese cabbage type winter habit is strong and weak, utilize plant height to identify the method that Chinese cabbage type winter rape winter habit is strong and weak particularly to a kind of.
Background technology
Global climate warms up in recent years, and winter crop Wintering Period temperature generally improves, and the frequency that extreme low temperature occurs reduces, and the persistent period reduces.Global wanning is particularly evident in northern China Han Han district, one of Chinese cabbage type winter rape research hot issue also just becoming Brassica campestris L research moving northward feasibility with the characteristic such as high yield, precocity, also it is North Dry Han Qu one of the important technique measure and strategy tackling global wanning challenge, wherein cold-resistant breed breeding is to develop the key that north winter rape produces, and the selection-breeding of cold-resistant kind first has to solve winter habit power authentication method simple, effective, low cost.
Conventional cold hardness evaluation adopts the authentication methods such as the statistics of field wintering rate, Physiology and biochemistry method and molecular marker.It is big that the statistics of field wintering rate is subject to extraneous anthropic factor, and accuracy is not high.
Physiology and biochemistry authentication method needs certain equipment, medicine, and the bad grasp of most of physiological and biochemical index, change is big, be not sufficiently stable, waste time and energy, cost is high, accuracy is not high.
Molecular labeling method is first to find closely linked molecular marker strong and weak with kind winter habit, then detects with this molecular marker, judges according to the presence or absence of key band.The method advantage is that testing result is more accurate, it is possible to achieve detection in the present age.Shortcoming is that the operation to molecular marker requires strictly, and exploitation labelling cost is high, only applies in minority unit at present.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing plant height to identify Chinese cabbage type winter rape winter habit power, accuracy is higher, and is easy to operation.
For achieving the above object, the technical solution adopted in the present invention is: a kind of method utilizing plant height to identify Chinese cabbage type winter rape winter habit power, specifically sequentially includes the following steps:
1) by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified at field planting, total Seedling number of plantation is more than 30 strains；
2) grow 60～65 days after emerging, measure the plant height of all seedling in Chinese cabbage to be identified type winter rape variety colony；
3) plant height according to all seedling recorded, carries out plant height standardization:
Z=X/Xmax
In formula: Z is plant height standardization；X is the plant height of the seedling recorded, unit mm；XmaxFor plant height the highest in colony, unit mm；
4) judge that individual plant winter habit is strong and weak according to plant height standardization: 0.9≤Z < 1 as IV grade, spring habit；0.5≤Z < 0.9 is III grade, weak winter habit；0.2≤Z < 0.5 is II grade, winter habit；Z < 0.2 is I grade, strong winter habit.
Authentication method of the present invention is by measuring the plant height of Chinese cabbage type winter rape colony, calculate plant height standardization and differentiate that Chinese cabbage type winter rape variety winter habit to be identified is strong and weak, the shortcoming overcoming the tradition statistics of wintering rate, Physiology and biochemistry and molecular labeling method, simple to operate, realize detection in the present age, differentiate that individual plant winter habit is strong and weak, laboratory condition and technology are required low, cost is low, reliable results.
Detailed description of the invention
Hereinafter principles of the invention and feature being described, example is served only for explaining the present invention, is not intended to limit the scope of the present invention.
The present invention utilizes plant height to identify the method that Chinese cabbage type winter rape winter habit is strong and weak, specifically sequentially includes the following steps:
1) field planting: by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified at field planting, due to strain number very little, can affect the reliability of result, so total Seedling number of plantation is more than 30 strains；
2) survey of growth and development: grow 60～65 days after emerging, measures the plant height of all seedling in Chinese cabbage to be identified type winter rape variety colony；
3) according to the plant height (X) of all seedling recorded, carry out plant height standardization (Z), be namely respectively divided by colony the highest plant height (X with the plant height (X) of seedling each in colonymax):
Z=X/Xmax
4) individual plant winter habit power judges: 0.9≤plant height standardization (Z) < 1 as IV grade, spring habit；0.5≤plant height standardization (Z) < 0.9 is III grade, weak winter habit；0.2≤plant height standardization (Z) < 0.5 is II grade, winter habit；Plant height standardization (Z) < 0.2 is I grade, strong winter habit.
Winter rape is after spring sowing, and its plant height is more high, and winter habit is more weak, otherwise winter habit is more strong.This judgement Main Basis is vernalization: if plant is entirely through vernalization, winter rape ability bolting, and normal growth reaches certain plant height；If plant vernalization is complete, winter rape energy bolting, but can not normal growth, plant height can not reach normal height；If plant can not pass through vernalization, winter rape can not bolting, be constantly in and nourish and grow, crawl in ground.Thus winter rape winter habit power can being divided into 4 grades: I grade, for strong winter habit, II grade is weak winter habit, and III grade is winter habit, and IV grade is spring habit.
5) colony's winter habit power judges: determining that colony's winter habit is strong and weak according to the ratio (B) of the Zhu Shuozhan colony of plant height standardization (Z) < 0.2,85.00%≤B, winter habit power rank I grade, for superpower winter variety；75%≤B < 85%, winter habit power rank II grade, for strong winter variety；65%≤B < 75%, winter habit power rank III grade, for winter variety；20%≤B < 65%, winter habit power rank IV grade is weak winter variety；B < 20%, winter habit power rank V grade, for spring varieties.
According to being entirely through vernalization strain number in experimental plot, winter rape, after spring sowing, judges that tested variety winter habit is strong and weak, Main Basis, under same environmental condition, the kind that winter habit is strong and weak different is notable by vernalization degree difference.Carry out winter habit division when natural vernalization and there is certain reasonability.
Winter rape is after spring sowing, and because of the difference that winter habit is strong and weak, different cultivars difference in plant height is notable, the little error caused on measuring, the reliability strong and weak without influence on judging winter rape winter habit.
Brassica campestris L winter habit is strong and weak relevant with vernalization characteristic, and the different genotypical sensitivity to temperature of winter resistance there are differences, and winter resistance is stronger, winter habit more by force, vernalization temperature required lower, the time is longer；Under same growing environment, the kind that winter resistance is strong and weak different, all can pass through vernalization, but vernalization degree varies causes, cause that the plant height of colony is uneven, according to this phenomenon (principle), both the difference of kind winter habit can have been identified, it is also possible to the winter habit identifying individual plant is strong and weak.
Embodiment: Shang Chuan town, Lanzhou in 2014 is tested
(1) field planting: by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified at field planting, total Seedling number is more than 30 strains；
(2) survey of growth and development: grow 60～65 days after emerging, measures all plant heights in Chinese cabbage to be identified type winter rape variety colony；
(3) plant height standardization (Z): according to measuring all plant heights in Chinese cabbage to be identified type winter rape variety colony, carry out plant height standardization (Z), namely it is respectively divided by colony the highest plant height (Xmax) with plant heights (X) all in colony, it may be assumed that (Z=X/Xmax)
4) individual plant winter habit power judges: 0.9≤plant height standardization (Z) < 1 as IV grade, spring habit；0.5≤plant height standardization (Z) < 0.9 is III grade, weak winter habit；0.2≤plant height standardization (Z) < 0.5 is II grade, winter habit；Plant height standardization (Z) < 0.2 is I grade, strong winter habit.
5) colony's winter habit power judges: determining that colony's winter habit is strong and weak according to the ratio (B) of the Zhu Shuozhan colony of plant height standardization (Z) < 0.2,85.00%≤B, winter habit power rank I grade, for superpower winter variety；75%≤B < 85%, winter habit power rank II grade, for strong winter variety；65%≤B < 75%, winter habit power rank III grade, for winter variety；20%≤B < 65%, winter habit power rank IV grade is weak winter variety；B < 20%, winter habit power rank V grade, for spring varieties.
By winter rape variety to be identified at field seeding at the beginning of last month of summer spring end, grow 60～65 days after emerging, measure Chinese cabbage to be identified type winter rape variety colony plant height, calculate plant height standard, it is determined that Chinese cabbage type winter rape variety winter habit power rank to be identified.Result is in Table 1.
Shang Chuan town, table Lanzhou in 12014 result of the test
。
Claims (1)
1. utilize plant height to identify the method that Chinese cabbage type winter rape winter habit is strong and weak, specifically sequentially include the following steps:
1) by Chinese cabbage type winter rape variety the end of spring and the beginning of summer to be identified at field planting, total Seedling number of plantation is more than 30 strains；
2) grow 60～65 days after emerging, measure the plant height of all seedling in Chinese cabbage to be identified type winter rape variety colony；
3) plant height according to all seedling recorded, carries out plant height standardization:
Z=X/Xmax
In formula: Z is plant height standardization；X is the plant height of the seedling recorded, unit mm；XmaxFor plant height the highest in colony, unit mm；
4) judge that individual plant winter habit is strong and weak according to plant height standardization: 0.9≤Z < 1 as IV grade, spring habit；0.5≤Z < 0.9 is III grade, weak winter habit；0.2≤Z < 0.5 is II grade, winter habit；Z < 0.2 is I grade, strong winter habit.
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| CN201610227970.4A CN105794479B (en) | 2016-04-13 | 2016-04-13 | A method of it is strong and weak using plant height identification Chinese cabbage type winter rape winter habit |
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| CN201610227970.4A CN105794479B (en) | 2016-04-13 | 2016-04-13 | A method of it is strong and weak using plant height identification Chinese cabbage type winter rape winter habit |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896979A (en) * | 2017-11-16 | 2018-04-13 | 甘肃农业大学 | The selection of the excellent plant type of winter habit Chinese cabbage type winter rape |
| CN108812110A (en) * | 2018-04-28 | 2018-11-16 | 甘肃农业大学 | Method using plant with respect to plant height identification cabbage type rape temperature-sensitive characteristic |
| CN110089368A (en) * | 2019-05-08 | 2019-08-06 | 甘肃农业大学 | A method of Winter Cole-wort winter resistance is identified by measurement hypocotyl length |
| CN110810173A (en) * | 2019-10-29 | 2020-02-21 | 甘肃农业大学 | Method for identifying cold resistance of cabbage type winter rape by root system characters |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104686148A (en) * | 2015-02-25 | 2015-06-10 | 滨州市作物研究所 | Method for identifying the springness and winterness of wheat varieties and regulating flower synchronization |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104686148A (en) * | 2015-02-25 | 2015-06-10 | 滨州市作物研究所 | Method for identifying the springness and winterness of wheat varieties and regulating flower synchronization |
Non-Patent Citations (5)
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| 余阳俊 等: "《中国园艺学会第四届青年学术讨论会论文集》", 31 December 2000 * |
| 冀天会 等: "综合顺序分类法鉴定小麦品种冬春性的研究", 《安徽农业科学》 * |
| 张灿军: "小麦冬春性鉴定研究", 《中国优秀硕士论文全文数据库》 * |
| 李长缨 等: "甘蓝冬性鉴定方法及标准的研究", 《华北农学报》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896979A (en) * | 2017-11-16 | 2018-04-13 | 甘肃农业大学 | The selection of the excellent plant type of winter habit Chinese cabbage type winter rape |
| CN108812110A (en) * | 2018-04-28 | 2018-11-16 | 甘肃农业大学 | Method using plant with respect to plant height identification cabbage type rape temperature-sensitive characteristic |
| CN110089368A (en) * | 2019-05-08 | 2019-08-06 | 甘肃农业大学 | A method of Winter Cole-wort winter resistance is identified by measurement hypocotyl length |
| CN110810173A (en) * | 2019-10-29 | 2020-02-21 | 甘肃农业大学 | Method for identifying cold resistance of cabbage type winter rape by root system characters |
| CN110810173B (en) * | 2019-10-29 | 2021-05-18 | 甘肃农业大学 | Method for identifying cold resistance of cabbage type winter rape by root system characters |
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Inventor after: Sun Wancang Inventor after: Guo Rendi Inventor after: Wang Jijun Inventor after: Pu Yuanyuan Inventor after: Ma Chi Inventor after: Dong Xiaoyun Inventor after: Bai Jing Inventor after: He Huili Inventor after: Hou Xianfei Inventor after: Li Xuecai Inventor after: Gu Yuanguo Inventor after: Liu Zigang Inventor after: Zeng Xiucun Inventor after: Wu Junyan Inventor after: Fang Yan Inventor after: Yang Gang Inventor before: Sun Wancang Inventor before: Hou Xianfei Inventor before: Li Xuecai Inventor before: Liu Zigang Inventor before: Zeng Xiucun Inventor before: Wu Junyan Inventor before: Fang Yan Inventor before: Yang Gang Inventor before: Guo Rendi |
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Granted publication date: 20190118 Termination date: 20200413 |