CN108782580A - A kind of bactericidal composition and application thereof of the rosickyite azoles and SSF 126 of prevention rice blast - Google Patents
A kind of bactericidal composition and application thereof of the rosickyite azoles and SSF 126 of prevention rice blast Download PDFInfo
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- CN108782580A CN108782580A CN201811103669.8A CN201811103669A CN108782580A CN 108782580 A CN108782580 A CN 108782580A CN 201811103669 A CN201811103669 A CN 201811103669A CN 108782580 A CN108782580 A CN 108782580A
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- rosickyite azoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to technical field of pesticide to be made more particularly to a kind of bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast of following raw material：Account for the water of the active constituent of composition weight 5% to 50%, the wetting dispersing agent for accounting for composition weight 2% to 6%, the thickener for accounting for composition weight 0.1% to 0.4%, the antifreezing agent for accounting for composition weight 3% to 5%, the antifoaming agent for accounting for composition weight 0.4% to 0.6% and surplus, the active constituent is the mixture of rosickyite azoles and SSF 126, and the weight ratio of rosickyite azoles and SSF 126 is（1~4）：（1~4）.Rosickyite azoles used in the present invention is absorbability rosickyite azoles fungicide, and SSF 126 is wide spectrum triazole bactericidal agent, and mutual mixture not will produce low touch, it can be synergistic, i.e. the activity of mixture is more than the activity of independent component, reduces dosage, slowing down resistance reduces cost.
Description
Technical field
The invention belongs to technical field of pesticide, are related to a kind of medicament composition for preventing agricultural disease, particularly relate to
And pharmaceutical agent combinations are configured to suspending agent, the prevention for rice blast in agricultural production.
Background technology
Rice blast is one of important disease of rice, can cause the significantly underproduction, the underproduction 40% ~ 50% when serious, even
No kernels or seeds are gathered, as in a year of scarcity.Each rice region in the world uniformly occurs.This disease has generation in various regions, wherein occured as with leaf portion, section portion it is more, after generation
The different degrees of underproduction, especially panicle blast or section pest can be caused to occur early and weigh, dead ears can be caused so that total crop failure, each rice production in China
Qu Junyou occurs.The underproduction 10% ~ 20% as the popular time-, the underproduction 40% ~ 50% when serious, or even No kernels or seeds are gathered, as in a year of scarcity.Germ mainly with
Conidium and mycelium are overwintering on straw and paddy.Next year generates conidium and is traveled in rice strain by means of wind and rain, and sprouting is invaded
People host extends to adjacent cells and falls ill, and forms primary infection focus.The conidium that sick portion is formed borrows wind and rain to propagate, then is invaded
Dye.Sowing infected seed can cause seedling pest.8 ~ 37 DEG C of mycelia growth temperature limit, 26 ~ 28 DEG C of optimum temperature.Sporogenesis temperature limit 10 ~
It is 35 DEG C, most suitable with 25 ~ 28 DEG C, 90% or more relative humidity.Spore germination with the presence of water and need to continue 6 ~ 8 hours.Thermophilic high humidity,
Be conducive to fall ill under rain, mist, dew existence condition.
2012, Guizhou road member biology plant pesticide research institute joint Beijing University of Chinese Medicine expert, Nanjing agriculture were big
It learns expert and beneficial one hundred pharmacy Miao Yao R&D teams successfully develops the innovative green bactericide of rosickyite azoles.Rosickyite azoles（Albendazole）It is
A kind of people's insecticide sterilization component, mixed morbidity evils a variety of to plant and pathogenic infection have inhibiting effect.Belong to absorbability third
Sulphur azole a new generation disinfectant use in agriculture has prevention & protection, interior suction treatment, infiltration conduction.Wide sterilization spectrum has stronger
The complementarity of the mode of action and bioactivity.For efficient, less toxic, real wide spectrum systemic fungicide, it may have activity is high, holds
The feature that the effect phase is long, absorbability is strong, spraying time is flexible, it to brown spot have favorable effect.
SSF 126 is methoxy acrylic bactericide, mitochondrial respiratory inhibitor, i.e., by cytochrome b and
Electronics transfer inhibits the breathing of mitochondria between C1.To 14- demethylations enzyme inhibitor, benzamides, dicarboxylic dihydrazides amine and benzene
And the bacterial strain of imidazoles generation resistance is effective.With protection, treats, roots out, permeates, systemic activity.
But either rosickyite azoles or SSF 126 single dose be not in use, the control effect for rice blast is shown
It writes, does not have apparent preventive and therapeutic effect.Therefore, rosickyite azoles or SSF 126 are further studied in terms of preventing rice blast
Using being of great significance.
Invention content
In view of this, the object of the present invention is to provide a kind of sterilizations of the rosickyite azoles and SSF 126 of prevention rice blast
Composition and application thereof, to solve the technical barrier that current rice blast is difficult to long control.
The present invention solves above-mentioned technical problem by the following technical programs：
A kind of bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, is made of following raw material：Account for combination
The active constituent of object weight 5% to 50%, accounts for composition weight 0.1% to 0.4% at the wetting dispersing agent for accounting for composition weight 2% to 6%
Thickener, account for composition weight 3% to 5% antifreezing agent, account for composition weight 0.4% to 0.6% antifoaming agent and surplus
Water, the active constituent are the mixture of rosickyite azoles and SSF 126, and the weight ratio of rosickyite azoles and SSF 126 is（1~4）：（1~
4）.
The weight ratio of the rosickyite azoles and SSF 126 is（1~2）：（1~4）.
The weight ratio of the rosickyite azoles and SSF 126 is 1：3.
The wetting dispersing agent is agriculture breast 600-1.
The thickener is xanthan gum.
The antifreezing agent is ethylene glycol.
The antifoaming agent is methanol.
It is described prevention rice blast rosickyite azoles and SSF 126 bactericidal composition, be with rosickyite azoles and SSF 126
Principle active component and insecticides adjuvant, excipients are configured to any one dosage form allowed on pesticide.
The dosage form of the bactericidal composition is suspending agent.
The rosickyite azoles of the prevention rice blast and the bactericidal composition of SSF 126 are preparing prevention plant mycosis
Application on evil germ killing drugs.
The rosickyite azoles of the prevention rice blast and the bactericidal composition of SSF 126 are preparing rice blast sterilization
Application on drug.
The beneficial effects of the present invention are：
First, the rosickyite azoles used in the present invention is absorbability rosickyite azoles fungicide, and SSF 126 is methoxy acrylic
Fungicide, mutual mixture not will produce it is low touch, can be synergistic, i.e., the activity of mixture is more than the activity of independent component, reduces
Dosage, slowing down resistance reduces cost.
Secondly, composition of the invention has good control effect to rice blast；Since synergy causes to use
Amount reduces, and can inhibit fungi to single formulation（Rosickyite azoles or SSF 126）It develops immunity to drugs.
Below beneficial effects of the present invention are further verified to test：
1, toxicity measures
1.1 Study on Fungicide Toxicity
With reference to farm-chemical indoor determination test rule, using mycelial growth rate method, measure for examination single dose to rice blast
Inhibiting rate.The PDA culture medium mixing melted with 4.5mL using mycelial growth rate method, 0.5mL liquids, pours into sterile training
It supports and band medicine culture medium flat plate is made in ware.After culture medium solidification, 1 bacteria cake for trying bacterium is put into each culture base plane（Directly
Diameter is 0.5cm）, so that bacteria cake is attached to media surface on one side with mycelia, often handle 3 repetitions, with alcohol solvent and contain
0.1% Tween 80 aqueous solution is control.After cultivating 72h at 25 DEG C, measured with crossing method for trying bacterium colony growth diameter.
Inhibiting rate is calculated according to the following formula.And by probit analysis find out the toxicity regression curves of salicyloyl enamine, hymexazo single doses with
EC50Value, SD and 95% confidence limit.
Mycelial growth inhibition rate（%）=（Compare colony growth diameter-processing colony growth diameter）/ control bacterium colony growth is straight
Diameter × 100
The complex role of 1.2 rosickyite azoles and SSF 126 is studied
Rosickyite azoles and SSF 126 are compounded：Two kinds of active compounds are configured to 4 by active ingredient:1,3:1,2:1,1:1,1:2,
1:3,1:4 totally 7 proportioning（Mass ratio）.Above-mentioned formula presses Study on Fungicide Toxicity method and carries out toxicity test respectively.
Mixture synergy is evaluated, with EC50Based on value, mixture joint is evaluated by calculating mixture co-toxicity coefficient
Type of action.
CTC120 be synergistic effect, 80≤CTC<120 be summation action, CTC<80 be antagonism.
2, test result
2.1 Study on Fungicide Toxicity results
The Toxicity Determination result of the 12 kinds of medicaments of table to rice blast
| Treatment agent | Virulence regression equation | EC50（mg·L–1） | Related coefficient | SD | CL0.95 |
| Rosickyite azoles | y = 1.3529x + 2.1311 | 128.0587 | 0.9578 | 0.1736 | 58.5035~128.0587 |
| SSF 126 | y = 1.0678x + 3.7865 | 13.7400 | 0.9414 | 0.2233 | 5.0151~37.6436 |
As it can be seen from table 1 EC of the rosickyite azoles to rice blast50For 128.0587 μ g/mL；SSF 126 is to rice rice blast
The EC of disease50For 13.7400 μ g/mL.
2 rosickyite azoles of table compounds the The Toxicity Determination result to rice blast with SSF 126
| Rosickyite azoles:SSF 126 | Virulence regression equation | Related coefficient | EC50（μg/mL） | Co-toxicity coefficient |
| 1:4 | y= 0.7548x + 4.2901 | 0.9237 | 8.7203 | 191.8090 |
| 1:3 | y = 0.7687x + 4.369 | 0.9481 | 6.6681 | 265.2546 |
| 1:2 | y = 0.6372x + 4.413 | 0.7955 | 8.2940 | 235.8398 |
| 1:1 | y= 1.0685x + 3.9185 | 0.9746 | 10.3222 | 240.4262 |
| 2:1 | y = 1.274x + 3.4325 | 0.9736 | 16.9335 | 200.4157 |
| 3:1 | y= 1.4743x + 2.9623 | 0.9721 | 23.7956 | 174.7256 |
| 4:1 | y= 1.4215x + 2.8383 | 0.9603 | 32.5563 | 147.6507 |
As shown in Table 2, rosickyite azoles and all proportions of SSF 126 compounding show higher synergistic effect, but different compoundings
Ratio coefficient of synergism difference.Wherein, the compound proportion of rosickyite azoles and SSF 126 is 1：When 3, co-toxicity coefficient highest is
265.2546.Show that the compounding of rosickyite azoles and SSF 126 has preferable effect to the prevention of rice blast.
Specific implementation mode
In order to facilitate the understanding of those skilled in the art, below in conjunction with embodiment, the present invention will be further described.
Embodiment is only to the invention for example, not being limitation of the invention, the step of not illustrated in embodiment
It is prior art, is not described in detail herein.
Embodiment one
Weigh rosickyite azoles 1kg, SSF 126 4kg, wetting dispersing agent（Agriculture breast 600-1）2kg, thickener（Xanthan gum）0.1kg,
Antifreezing agent（Ethylene glycol）3kg, antifoaming agent（Methanol）0.4kg complements to 100kg with water, by above-mentioned raw materials at normal temperatures through high speed
Shear-mixed is uniform, and uniformly mixed material is then put abrading-ball 2h under room temperature in the ball mill, and Bactericidal composite of the present invention is made
The suspending agent of object.
Embodiment two
Weigh rosickyite azoles 40kg, SSF 126 10kg, wetting dispersing agent（Agriculture breast 600-1）6kg, thickener（Xanthan gum）
0.4kg, antifreezing agent（Ethylene glycol）5kg, antifoaming agent（Methanol）0.6kg complements to 100kg, at normal temperatures by above-mentioned raw materials with water
It is uniformly mixed through high speed shear, uniformly mixed material is then put into abrading-ball 3h under room temperature in the ball mill, the obtained present invention kills
The suspending agent of bacteria composition.
Embodiment three
Weigh rosickyite azoles 10kg, SSF 126 30kg, wetting dispersing agent（Agriculture breast 600-1）3kg, thickener（Xanthan gum）
0.2kg, antifreezing agent（Ethylene glycol）4kg, antifoaming agent（Methanol）0.5kg complements to 100kg, at normal temperatures by above-mentioned raw materials with water
It is uniformly mixed through high speed shear, uniformly mixed material is then put into abrading-ball 2.2h under room temperature in the ball mill, the present invention is made
The suspending agent of bactericidal composition.
Example IV
Weigh rosickyite azoles 10kg, SSF 126 20kg, wetting dispersing agent（Agriculture breast 600-1）4kg, thickener（Xanthan gum）
0.3kg, antifreezing agent（Ethylene glycol）3kg, antifoaming agent（Methanol）0.4kg complements to 100kg, at normal temperatures by above-mentioned raw materials with water
It is uniformly mixed through high speed shear, uniformly mixed material is then put into abrading-ball 2.5h under room temperature in the ball mill, the present invention is made
The suspending agent of bactericidal composition.
Embodiment five
Weigh rosickyite azoles 15kg, SSF 126 15kg, wetting dispersing agent（Agriculture breast 600-1）4kg, thickener（Xanthan gum）
0.3kg, antifreezing agent（Ethylene glycol）4kg, antifoaming agent（Methanol）0.5kg complements to 100kg, at normal temperatures by above-mentioned raw materials with water
It is uniformly mixed through high speed shear, uniformly mixed material is then put into abrading-ball 2.5h under room temperature in the ball mill, the present invention is made
The suspending agent of bactericidal composition.
Embodiment six
Weigh rosickyite azoles 27kg, SSF 126 9kg, wetting dispersing agent（Agriculture breast 600-1）3kg, thickener（Xanthan gum）
0.2kg, antifreezing agent（Ethylene glycol）5kg, antifoaming agent（Methanol）0.6kg complements to 100kg, at normal temperatures by above-mentioned raw materials with water
It is uniformly mixed through high speed shear, uniformly mixed material is then put into abrading-ball 3h under room temperature in the ball mill, the obtained present invention kills
The suspending agent of bacteria composition.
Embodiment seven
Weigh rosickyite azoles 28kg, SSF 126 14kg, wetting dispersing agent（Agriculture breast 600-1）5kg, thickener（Xanthan gum）
0.3kg, antifreezing agent（Ethylene glycol）4kg, antifoaming agent（Methanol）0.5kg complements to 100kg, at normal temperatures by above-mentioned raw materials with water
It is uniformly mixed through high speed shear, uniformly mixed material is then put into abrading-ball 3h under room temperature in the ball mill, the obtained present invention kills
The suspending agent of bacteria composition.
The rosickyite azoles of prevention rice blast and the bactericidal composition and application thereof of SSF 126 of the present invention has passed through tool
The embodiment of body is described.Those skilled in the art can use for reference the appropriate feed change of present disclosure, process conditions
Etc. links realize corresponding other purposes, correlation changes all without departing from present disclosure, all similar replacements and
Change is it will become apparent to those skilled in the art that be considered as being included within the scope of the present invention.
Claims (10)
1. a kind of bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, it is characterised in that：It is by following original
Material is made：It accounts for the active constituent of composition weight 5% to 50%, the wetting dispersing agent for accounting for composition weight 2% to 6%, account for composition
The thickener of weight 0.1% to 0.4%, accounts for disappearing for composition weight 0.4% to 0.6% at the antifreezing agent for accounting for composition weight 3% to 5%
The water of infusion and surplus, the active constituent are the mixture of rosickyite azoles and SSF 126, the weight of rosickyite azoles and SSF 126
Measuring ratio is（1~4）：（1~4）.
2. the bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, feature exist as described in claim 1
In：The weight ratio of the rosickyite azoles and SSF 126 is（1~2）：（1~4）.
3. the bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast as claimed in claim 1 or 2, feature
It is：The weight ratio of the rosickyite azoles and SSF 126 is 1：3.
4. the bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, feature exist as described in claim 1
In：The wetting dispersing agent is agriculture breast 600-1.
5. the bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, feature exist as described in claim 1
In：The thickener is xanthan gum.
6. the bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, feature exist as described in claim 1
In：The antifreezing agent is ethylene glycol.
7. the bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, feature exist as described in claim 1
In：The antifoaming agent is methanol.
8. the bactericidal composition of the rosickyite azoles and SSF 126 of prevention rice blast, feature exist as described in claim 1
In：The bactericidal composition is configured to pesticide using rosickyite azoles and SSF 126 as principle active component and insecticides adjuvant, excipients
Any one dosage form of upper permission.
9. the bactericidal composition of the rosickyite azoles and SSF 126 of the prevention rice blast as described in claim 1 or 8, feature
It is：The dosage form of the bactericidal composition is suspending agent.
10. the bactericidal composition for the rosickyite azoles and SSF 126 for preventing rice blast described in claim 1 ~ 9 any one exists
Prepare the application on rice blast germ killing drugs.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107333791A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of OIT compounding |
| CN113632795A (en) * | 2021-05-07 | 2021-11-12 | 广东省农业科学院果树研究所 | Application of albendazole sulfoxide in preparation of pesticide bactericide for preventing and treating citrus postharvest chloromycete |
| CN113662002A (en) * | 2021-05-07 | 2021-11-19 | 广东省农业科学院果树研究所 | Application of albendazole in preparation of pesticide bactericide for preventing and treating rice blast disease |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333791A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of OIT compounding |
| CN113632795A (en) * | 2021-05-07 | 2021-11-12 | 广东省农业科学院果树研究所 | Application of albendazole sulfoxide in preparation of pesticide bactericide for preventing and treating citrus postharvest chloromycete |
| CN113662002A (en) * | 2021-05-07 | 2021-11-19 | 广东省农业科学院果树研究所 | Application of albendazole in preparation of pesticide bactericide for preventing and treating rice blast disease |
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Application publication date: 20181113 |