CN113662002A

CN113662002A - Application of albendazole in preparation of pesticide bactericide for preventing and treating rice blast disease

Info

Publication number: CN113662002A
Application number: CN202110496702.3A
Authority: CN
Inventors: 彭程; 陆育生; 邱继水; 常晓晓
Original assignee: Pomology Research Institute Guangdong Academy of Agricultural Sciences
Current assignee: Pomology Research Institute Guangdong Academy of Agricultural Sciences
Priority date: 2021-05-07
Filing date: 2021-05-07
Publication date: 2021-11-19

Abstract

The invention discloses an application of albendazole shown in formula 1 in preparing a pesticide bactericide for preventing and treating rice blast disease:

the novel application of the prochloraz in preventing and controlling rice blast germs as a pesticide bactericide is discovered for the first time, and is obviously higher than that of a commercial bactericide prochloraz.

Description

Application of albendazole in preparation of pesticide bactericide for preventing and treating rice blast disease

The technical field is as follows:

the invention relates to application of albendazole in preparation of a pesticide bactericide for preventing and treating rice blast disease.

Background art:

the rice blast is caused by Pyricularia oryzae (Phynicoularia gisea) belonging to fungi of the subdivision Deuteromycotina, also known as Pyricularia oryzae (Pyricularia oryzae).

At present, the domestic rice blast prevention and control strategy is mainly based on planting high-yield disease-resistant varieties, takes field cultivation management as the center, and is assisted by proper and timely medicament prevention and control. Planting disease-resistant varieties is the most economic and effective strategy for preventing and treating rice blast, but due to the influence of factors such as pathogenicity variation of rice blast germs and the like, resistance of some disease-resistant varieties begins to be gradually lost after the rice blast germs are planted in fields for 3-5 years. Although breeding resistant varieties is the most economical and environmentally friendly method for controlling the diseases, the use of chemical agents in the case of lack of resistant varieties or incomplete resistance is still the main means for controlling fungal diseases of crops. Albendazole is used medically to expel roundworms, pinworms, tapeworms, whipworms, hookworms, strongyloides stercoralis, etc. However, no related research on the rice blast disease resistance of albendazole exists at present.

The invention content is as follows:

the invention aims to provide application of imidazole derivative A albendazole in preparing pesticide bactericide for preventing and treating rice blast disease.

The invention is realized by the following technical scheme:

application of albendazole shown in formula 1 in preparation of pesticide bactericide for preventing and treating rice blast disease:

when in use, the concentration of albendazole is 2.5ug/mL to 10 ug/mL.

The invention has the following beneficial effects: the invention discovers the new application of the imidazole derivative A albendazole serving as a pesticide bactericide in preventing and controlling rice blast germs for the first time, and the safety of the imidazole derivative A albendazole is obviously higher than that of a commercial bactericide prochloraz.

Description of the drawings:

FIG. 1 is a graph showing the effect of different concentrations of albendazole on the growth of Pyricularia oryzae.

The specific implementation mode is as follows:

the following is a further description of the invention and is not intended to be limiting.

Example 1: inhibition of rice blast bacteria by albendazole

(1) Test drug

Albendazole, prochloraz (positive control compound, commercial pesticide).

(2) Test fungal species

The number of the tested fungus strains is 1, and the fungi strains are rice blast germs.

(3) Determination of anti-plant-fungal Activity

Preparation of test bacterium plates: under aseptic condition, respectively inoculating pure test bacteria to a sterile PDA culture medium plate by a dense wave line drawing method, culturing at 27 deg.C for about one week, and allowing the plate to grow bacterial lawn.

Preparation of the culture medium and sterilization thereof: the culture medium is PDA solid culture medium, and the formula is as follows: 200g of potato, 20g of glucose, 20g of agar and 1000mL of tap water. The preparation process comprises the following steps: cutting peeled potato into 5mm square pieces, adding tap water, boiling for 20min, filtering with 8 layers of gauze, adding corresponding amount of glucose and agar powder into the filtrate, heating and stirring to dissolve, adding tap water to desired volume, packaging into 250mL triangular bottles, sealing (each bottle contains 100mL culture medium), and sterilizing with autoclave at 115 deg.C for 30 min.

Preparing a liquid medicine: the compounds prochloraz and albendazole were weighed, 1.00mg each, into a 1.5mL sterile centrifuge tube, and 1mL of ldmso was added to dissolve each.

Preparation of a culture medium with medicine: heating and melting a culture medium in a sterilized triangular flask, cooling to about 50 ℃, respectively adding the prepared liquid medicine on a clean bench through aseptic operation, slightly shaking the triangular flask to uniformly distribute the liquid medicine in the culture medium, preparing albendazole into a culture medium with medicine with a final concentration of 10ug/mL, immediately pouring the medium into 5 sterile culture dishes (20 mL of culture medium in each culture dish) while the medium is hot to prepare flat plates with uniform thickness, and marking, wherein prochloraz is a positive control; in the same procedure, plates of negative control DMSO (0.1mL directly in 100mL sterile medium) were prepared and labeled.

And (3) testing the bacteriostatic rate: under the aseptic condition, punching a certain amount of fungus cakes on a test fungus flat plate by using an aseptic puncher, taking the fungus cakes by using an inoculating loop holder, placing the fungus cakes on a prepared culture medium with medicine, enabling the hyphae to face downwards, marking 1 fungus cake per dish (three times), placing the fungus cakes in a thermostat at 27 ℃ for culturing for 72 hours, taking out a culture, measuring the diameter of a bacterial colony by using a caliper (measuring the cross quantity twice, measuring all three repeated flat plates, taking the average number of the three repeated flat plates), calculating the bacteriostasis rate according to the formula (1), and obtaining the calculation result of the plant fungus resisting activity of albendazole at the concentration of 10ug/mL in the table 1.

Inhibition rate (negative control growth diameter-treated growth diameter)/(negative control growth diameter-5) × 100% … (1).

As can be seen from Table 1, the albendazole compound of the present invention has a good bactericidal effect on Pyricularia oryzae, wherein the concentration of albendazole is 10ug/mL and can reach 100%. See fig. 1.

TABLE 1 inhibition of plant fungi by albendazole and prochloraz compounds

Compound (I)	Bacteriostatic ratio (%)
		Prochloraz	100
Albendazole	100

Example 2:

reference example 1, except that the formulation of the drug solution: the compounds prochloraz and albendazole were weighed, 1.00mg each, into a 1.5mL sterile centrifuge tube, and 1mL of ldmso was added to dissolve each. Preparation of a culture medium with medicine: heating and melting the culture medium in a sterilized triangular flask, cooling to about 50 ℃, respectively adding the prepared liquid medicine on a clean bench through aseptic operation, slightly shaking the triangular flask to uniformly distribute the liquid medicine in the culture medium, preparing the albendazole into culture mediums with medicines with final concentrations of 10ug/mL, 5ug/mL, 2.5ug/mL, 1.25ug/mL and 0.625ug/mL, immediately pouring the mixture into 5 sterile culture dishes (20 mL of each culture dish) while the mixture is hot, preparing a flat plate with uniform thickness, and marking; in the same procedure, plates of negative control DMSO (0.1mL directly in 100mL sterile medium) were prepared and labeled. The results of the calculation of the inhibitory activity of albendazole against rice blast germs at different concentrations are shown in tables 2 and 3.

TABLE 2 inhibitory Activity of albendazole against Pyricularia oryzae at various concentrations (48h)

The virulence equation is y-4.0745X +5.7413, and the correlation coefficient is R²＝0.982，EC₅₀＝0.6577ug/ml。

TABLE 3 inhibitory Activity of Albendazole at different concentrations against Pyricularia oryzae (72h)

The virulence equation is

y is 4.0745X +5.7413 and the correlation coefficient is R²＝0.982，EC₅₀＝0.6029ug/ml。

The albendazole of the invention shows excellent inhibition effect on rice blast germs, and the EC of the albendazole₅₀The average value was 0.6303. mu.g/mL. In conclusion, the albendazole disclosed by the invention shows remarkable inhibitory activity on rice blast fungi.

Claims

1. Application of albendazole shown in formula 1 in preparation of pesticide bactericide for preventing and treating rice blast disease:

2. the application of albendazole in preparing pesticide bactericide for preventing and treating rice blast disease as claimed in claim 1, wherein the concentration of albendazole is 2.5ug/mL-10 ug/mL.