CN108781952A - The propagation method of Flos micheliae Albae - Google Patents
The propagation method of Flos micheliae Albae Download PDFInfo
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- CN108781952A CN108781952A CN201810290555.2A CN201810290555A CN108781952A CN 108781952 A CN108781952 A CN 108781952A CN 201810290555 A CN201810290555 A CN 201810290555A CN 108781952 A CN108781952 A CN 108781952A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses the propagation methods of Flos micheliae Albae, including：Greenhouse pre-treatment, preparing nursery bed, prepare cuttings, cuttage, insert after manage, it carries out disinfection first to greenhouse, close plastic shed, then garden mould, peat, perlite and pine needle layer are mixed into seedbed in proportion, it dips after another clip cuttings and is kept in after preserving liquid, when cuttage by cuttings is of short duration be soaked into rooting agent solution after carry out cuttage, water management, temperature management, sterilizing, the relevant management of fertilising are carried out after the completion of cuttage successively, 75~85% fringe bar can carry out hardening after taking root, can be transplanted after the completion of hardening.It has the beneficial effect that：This propagation method is simple and practicable, Flos micheliae Albae cuttings transpiration dehydration can not only be effectively prevent, it has been also convenient for that cuttings concentrate preserving simultaneously to allocate with management, rooting agent solution is dipped before cuttage can preserve the biological activity of rooting agent solution significantly, it may advantageously facilitate Flos micheliae Albae to take root and survive, substantially increase the cuttage survival rate of Flos micheliae Albae.
Description
Technical field
The present invention relates to yulan breeding fields, more particularly, to the propagation method of Flos micheliae Albae.
Technical background
Flos micheliae Albae（Scientific name：Michelia alba DC.）, it is the kind that white flowers are opened in yulan magnolia.Also known as lily magnolia, yulan
Deng.Deciduous tree, up to 17 meters, the flowers and trees of Chinese Famous, important flowering tree of northern early spring, Shanghai City city flower have 2500
The cultivation history in or so year, for ornamental tree rare in flower garden.Ancient times is mostly transplanted before pavilion, platform, building, pavilion.Now be more common in gardens,
Isolated planting in factories and miness, scattered planting, or make shade tree in both sides of the road.The north, which also has, makees miniature gardening potting.Cultivation has now been introduced a fine variety all over the world
Training.Flos micheliae Albae deciduous tree, up to 17 meters, branch is extensively opened up, and is in wealthy umbrella shape tree crown；30 centimetres of the diameter of a cross-section of a tree trunk 1.3 meters above the ground；Bark grey；Rubbing branches and leaves has virtue
It is fragrant；Spray and bud are close by the micro- pubescence of yellowish white, and hair gradually falls off when old.The thin keratin of leaf, oblong or drapes over one's shoulders needle-shaped ellipse, long
It is 10~27 centimetres, 4~9.5 centimetres wide, apex grow tapering or shape of tail it is tapering, base portion wedge shape, above it is hairless, below sparsely grow it is micro- soft
Hair, two sides net vein is apparent when doing；Petiole grows 1.5~2 centimetres, dredges by micro- pubescence；Stipule trace is several up to petiole middle part.It is grey
Color is extremely fragrant；Tapel 10, lanceolar is 3~4 centimetres long, 3~5 millimeters wide；The connective of stamen stretches out long tip；Gynoecium quilt
Micro- pubescence, gynoecium handle are about 4 millimeters；Carpel is most, usual partial agenesis, with the extension of holder, Xing Cheng Follicle when ripe
The aggregate fruit of radish sparsely grow；Cerise when Follicle radish is ripe.4~September of florescence, summer is in full bloom, usually shaky.
The prior art such as application publication number is the Chinese invention patent of 106550689 A of CN, discloses a kind of Flos micheliae Albae skewer
Method for culturing seedlings is inserted, is included the following steps：A, cuttings selects；B, cuttage prepares；C, cuttage；D, it is managed after cuttage.The inventive method
Using semi-lignified spray as cuttings, cuttings lower end was impregnated before cuttage with growth regulator, and entered seedbed in cuttage
Later, growth regulator is added dropwise in the matrix also around cuttings, carries out interval sprinkling water mist to cuttings, can effectively keep shading
Humidity in canopy, the heat treatment to seedbed can prevent temperature control to the influence of cuttings, ensure the normal life of cuttings
Root environment, the cottage method step is simple, facilitates that management, period are short, survival rate is high, can cultivate a large amount of childrens in a short time
Seedling.But cuttings completes direct preservation in the inventive method, cuttings can be caused to rot, infection, dehydration, withered etc., no
Conducive to subsequent cuttage process, and cuttings is soaked into take out after 2~4h of immersion in growth regulator before cuttage and is dried, grown
Heteroauxin or methyl α-naphthyl acetate in conditioning agent not completely into the light degradation of heteroauxin or methyl α-naphthyl acetate in plant, can be caused and
Regulatory function is lost, in addition, growth regulator to be directly added drop-wise to also undesirable in the matrix around cuttings, the oxidation in soil
The complex environments such as substance can cause growth regulator significantly to degrade, and remaining undegradable part can also promote miscellaneous in plant
The swift and violent growth of grass, therefore all it is unfavorable for taking root and surviving for yulan.
Invention content
The purpose of the present invention is to provide the propagation methods of Flos micheliae Albae, this propagation method is simple and practicable, environmental sound, no
It can only be effectively prevent Flos micheliae Albae cuttings transpiration dehydration, while being also convenient for that cuttings preserve and having managed allotment, skewer concentratedly
The biological activity of rooting agent solution can be preserved significantly by dipping rooting agent solution before inserting, and be may advantageously facilitate Flos micheliae Albae and taken root and deposit
It is living, substantially increase the cuttage survival rate of Flos micheliae Albae.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：The propagation method of Flos micheliae Albae, packet
It includes：Greenhouse pre-treatment, preparing nursery bed, prepare cuttings, cuttage, insert after manage, specifically include following steps：
Greenhouse pre-treatment：Greenhouse is cleared up before cuttage, removes the sundries such as moss, lichens, the residual matrix in anti-grass cloth；It is right
Skylight, shady net, wind turbine, wet curtain, control water system etc. are overhauled, and ensure greenhouse normal operation during cuttage；Fine day to greenhouse into
Row sterilizing sprays anti-grass cloth and greenhouse film, closing greenhouse close plastic shed 12 with 300~400 times of diluted fenaminosulf aqueous solutions
~for 24 hours；Sterilizing is carried out before cutting propagation to greenhouse to be conducive to provide a less toxic growing environment, while greenhouse for yulan
Facilitate the growth factors such as control temperature, humidity, illuminance；
Preparing nursery bed：By garden mould, peat, perlite and pine needle layer according to 1:0.4~0.5:0.2~0.3:0.3~0.5 ratio
Example stirs evenly, and pier is real after being packed into 50 hole seed plates of 7~10cm depths, and often 5 disk of row, is paved into seedbed, sprays, 800~1000 times
Fenaminosulf solution sterilizing；Garden mould, rich in humus, probiotics, is suitably for newborn yulan seedling and provides sufficient support with pine needle layer
Point, while peat and perlite can be effectively improved the soil porosity and soil texture of garden mould and pine needle layer, make it pine is close to have
Degree, water holding getting fat；
Prepare cuttings：The branch for selecting no disease and pests harm, robust growth, neat and consistent, bud full, in early morning clip cuttings a length of 8
~12cm, retains 2~3 buds, upper cut straight snips, and clip is away from the 1st 1~2cm of bud, and lower cut is below the section at 0.5~0.8cm
Tiltedly cut；Cuttings is soaked into 10~15s in 2~4 DEG C of preservation liquid in addition to bud-leaf, it is temporary that taking-up is placed on cold and moisture location；Clearly
Morning clip cuttings can be scattered and disappeared with reducing cuttings excessive water to avoid high temperature and strong illumination, impregnate cuttings with cryopreservation solution
Branch simultaneously can effectively prevent cuttings transpiration dehydration in cold and moisture location is temporary, while can also facilitate and be concentrated to cuttings
Management allotment；
Cuttage：Temporary cuttings is taken out from cold and moisture location, cuttings is integrally soaked into except bud-leaf in addition to 20 in rooting agent solution~
Cuttings is gently inserted into matrix, per 1, cave cuttings, with the wood thicker than cuttings by 25s after 3~4s of standing after taking-up with 60~70% length
Cuttings is inserted into hole after stick hole pounding and grave mound, the unification of fringe bar bud-leaf direction spray permeable, inspection immediately after one wheel fringe bar has been inserted
Whether there is fringe bar to rush askew or blade face by water to be attached in matrix；It can effectively prevent cutting medium to carry out cuttage after wooden stick hole pounding
Cuttings is caused to scrape and is worn, is conducive to improve cuttage rooting quality and quantity, after cuttings is dipped rooting agent solution, root-growing agent
Effective physiological compositions in solution can be by enhancing cell metabolism come the plasticity of callus and skin zone's cell, to promote
This two-part cell growth, elongation make its tachyauxesis, differentiation, finally grow root system, complete to take root；
It is managed after inserting：To carry out water management, temperature management, sterilizing, the relevant management of fertilising after the completion of cuttage successively, 75
~85% fringe bar can carry out hardening after taking root, and cuttage seeding is transferred to together with hole tray on overhead seedbed, is poured 1 time every 2~4d
Water makes matrix the state of semi-wet occur, opens greenhouse skylight, is stepped up ventilation time and shortens the shading time, improves
The adaptability of Flos micheliae Albae seedling, 7~10d of hardening can be transplanted；The phases such as good moisture, temperature, sterilizing, fertilising
Management is closed, can not only accelerate Flos micheliae Albae and take root growth, makes its root system stalwartness, while additionally aiding the growth of seedling aerial part
Development, accelerates its growth, and by that can be transplanted after short-term hardening, cuttage survival rate is higher, and growth of seedling is healthy and strong.
Preferably, preparing the substance that the preservation liquid in cuttings step contains following content：2.5~3.0% glucan,
1.0~1.2% potassium dihydrogen phosphate, the gibberellin of 12~15ppm, the brassin lactones of 15~20ppm, 0.2~0.3% single silicon
(4S) -2,2- dimethyl -1,3-dioxolane -4- formaldehyde of acid, the ascorbic acid of 40~50ppm, 11~12ppm, surplus are
Water；A thin layer of water-soluble liquid film can be formed on cuttings surface by preserving liquid, have the function of moisturizing, fungi-proofing, Flos micheliae Albae cuttings is cut
Contain tannin at mouthful, tannin is easily oxidized to form one layer of fine and close isolation film, which can not only influence subsequent growth element
The entrance of solution can also cause to obstruct to the newborn root system of callus, reduce rooting rate and survival rate, preserve anti-bad in liquid
Hematic acid can seize oxidation material, prevent excessive oxidation of the oxidation material to tannin, meanwhile, (4S) -2 micro in liquid is preserved,
2- dimethyl -1,3-dioxolane -4- formaldehyde can form water-soluble hydrate, which can in the presence of single silicic acid
Phenol-formaldehyde reaction occurs with tannin, to reduce the oxidation of tannin, the thickness of the isolation film after reduction tannin oxidation does not interfere with
Callus is taken root, and survival rate is improved.
Preferably, containing the substance of following content in rooting agent solution in cuttage step：2.5~3.0% sucrose,
The gibberellin of 20~25ppm, the aspirin of 12~15ppm, the ascorbic acid of 20~30ppm, 1.6~2.0% honey, 30~
The 6- benzyls aminoadenine of 40ppm, the heteroauxin of 50~60ppm, 5~8ppm (R)-(-) -2,2- diphenyl cyclopentanol,
0.5~0.6% urea, 0.3~0.4% potassium dihydrogen phosphate, 0.2~0.3% boric magnesium fertilizer, surplus is distilled water；Callus
Rapid differentiation with the initial stage of Adventitious root initiation, cell makes soluble sugar content drastically decline with growth, and sucrose can be with honey
Root system provides required sugar, and the crescendo of root absorbability and photosynthesis are restored later, and sugared content is gradually increasing again；It is red mould
Element, aspirin help to sterilize, and can prevent callus from rotting, prevent root system from infecting；6- benzyls aminoadenine, indoles second
Acid can act on cell, cambial cell can be stimulated to divide, and xylem, phloem cell differentiation, promotion is promoted to exceed injured tissue
Differentiation, the morphogenesis for adjusting callus, hestening rooting；But heteroauxin is easily aoxidized to lose its physiological function,
(R)-(-) -2, with heteroauxin complex reaction can occur for 2- diphenyl cyclopentanol, produce metastable complex compound, to
It can prevent heteroauxin from turning to indoles by photooxidation and losing adjustment effect, which can be used as after entering in plant plants
A kind of storage form of heteroauxin in object, under proper condition, complex compound can be changed into trip via solution complex reaction
It is release, it is transferred to site of action through transport and works；The complex compound of heteroauxin and (R)-(-) -2,2- diphenyl cyclopentanol exists
It may advantageously facilitate the secretion of superoxide dismutase in plant, superoxide dismutase, which plays the development of adventitious root, to be promoted to make
With the elongation and growth of adventitious root can be effectively facilitated.
Preferably, water management is by adjusting conditions such as spray time, interval time, ventilation situations, ensure cuttage
Greenhouse indoor humidity is 90~95%, and a small amount of multiple principle is taken in spraying, has mist pearl without dripping for degree with cuttings surface；When
Cuttings will suitably reduce water spray number when starting to take root, and with sharp cuttage rooting, prevent root system from rotting.
Preferably, temperature management is by switching the facilities such as sunshade net, skylight, wet curtain, it is ensured that cuttage greenhouse Indoor Temperature
Degree is maintained between 27~32 DEG C, and natural temperature is kept during hardening.
Preferably, sterilizing operation is：Dusk after 12~14d of cuttage closes greenhouse spraying, with 1000~1500
Times carbendazim solution sprays seedbed, and carry out disinfection sterilization, and every 15~20d is carried out 1 time later；Sterilizing can be sterilized, be prevented
Disease is spread, and reduces cuttings rotting rate, reduces cuttings pest and disease damage infection rate, improves cuttage success rate and condition of rooting；
Preferably, fertilizing operation is：After cuttage 3~4 weeks, every 2 weeks, stop spraying in the dusk, blade face moisture content is used after drying
0.2~0.3% potassium dihydrogen phosphate or 0.1~0.2% urea or nursery stock liquid fertilizer special carry out foliage dressing；Page fertilising can be with
It is relatively straightforward to provide nutrient for seedling.
Compared with the prior art, the advantages of the present invention are as follows：
1）The ascorbic acid preserved in liquid can seize oxidation material, prevent excessive oxidation of the oxidation material to tannin, meanwhile, it protects
Micro (4S) -2,2- dimethyl -1,3-dioxolane -4- formaldehyde can form water-soluble hydrate in liquid storage, the hydration
In the presence of single silicic acid with tannin phenol-formaldehyde reaction can occur for object, to reduce the oxidation of tannin, after reduction tannin oxidation
The thickness of isolation film does not interfere with callus and takes root, and improves survival rate；
2）(R)-(-) -2, with heteroauxin complex reaction can occur for 2- diphenyl cyclopentanol, produce metastable complexing
Object loses adjustment effect so as to prevent heteroauxin from turning to indoles by photooxidation, which can after entering in plant
Using a kind of storage form as heteroauxin in plant, under proper condition, complex compound can via solution complex reaction and
It is changed into sequestered, being transferred to site of action through transport works；
3）The complex compound of heteroauxin and (R)-(-) -2,2- diphenyl cyclopentanol may advantageously facilitate superoxides in plant
The secretion of mutase, superoxide dismutase play a driving role to the development of adventitious root, can effectively facilitate the elongation of adventitious root with
Grow；
4）In the method for the present invention, Flos micheliae Albae cuttings is impregnated and carries out cuttage after preserving liquid, can not only effectively prevent cuttings rising
Dehydration, while can also facilitate and centralized management allotment is carried out to cuttings, rooting agent solution is dipped before cuttage to be preserved significantly
The biological activity of rooting agent solution may advantageously facilitate Flos micheliae Albae and take root and survive, and the method for the present invention is easy to operate, to ring
Border is harmless, is a kind of propagation method of the Flos micheliae Albae of high-survival rate.
Specific implementation mode
The present invention program is described further below by embodiment：
Embodiment 1：
The propagation method of Flos micheliae Albae, specifically includes following steps：
1）Greenhouse is cleared up before cuttage, removes the sundries such as moss, lichens, the residual matrix in anti-grass cloth；To skylight, shade
Net, wind turbine, wet curtain, control water system etc. are overhauled, and ensure greenhouse normal operation during cuttage；Fine day carries out sterilization to greenhouse and disappears
Poison sprays anti-grass cloth and greenhouse film, closing greenhouse close plastic shed 12h with 300 times of diluted fenaminosulf aqueous solutions；2）By garden mould, mud
Charcoal, perlite and pine needle layer are according to 1:0.4: 0.2:0.3 ratio stirs evenly, and is packed into pier after 50 hole seed plates of 7cm depths
Real, often 5 disk of row, is paved into seedbed, sprays, 800 times of fenaminosulf solution sterilizings；3）Select no disease and pests harm, robust growth, neat
Unanimously, the full branch of bud retains 2 buds, upper cut straight snips, clip is away from the 1st bud in the early morning a length of 8cm of clip cuttings
1cm, lower cut are tiltedly cut at 0.5cm below the section；Cuttings is soaked into 10s in 2 DEG C of preservation liquid in addition to bud-leaf, taking-up is placed on
Cold and moisture location is temporary；4）Temporary cuttings is taken out from cold and moisture location, it is molten that cuttings except bud-leaf in addition to is integrally soaked into root-growing agent
Cuttings is gently inserted into matrix, per 1, cave cuttings, with the wooden stick thicker than cuttings by 20s in liquid after standing 3s after taking-up with 60% length
Cuttings is inserted into hole after hole pounding and grave mound, fringe bar bud-leaf direction are unified, after a wheel fringe bar has been inserted, sprays immediately permeable, inspection is
No have fringe bar to rush askew or blade face by water to be attached in matrix；5）After the completion of cuttage water management carried out successively, temperature management, killed
Bacterium disinfection, relevant management of applying fertilizer, 75% fringe bar can carry out hardening after taking root, and cuttage seeding is transferred to together with hole tray overhead
On seedbed, 1 water is poured every 2d, matrix is made the state of semi-wet occur, greenhouse skylight is opened, is stepped up ventilation time simultaneously
Shorten the shading time, improve the adaptability of Flos micheliae Albae seedling, hardening 7d can be transplanted.
Embodiment 2：
The propagation method of Flos micheliae Albae, including：Greenhouse pre-treatment, preparing nursery bed, prepare cuttings, cuttage, insert after manage, specifically include
Following steps：
1）Greenhouse pre-treatment：Greenhouse is cleared up before cuttage, removes the sundries such as moss, lichens, the residual matrix in anti-grass cloth；
Skylight, shady net, wind turbine, wet curtain, control water system etc. are overhauled, ensure greenhouse normal operation during cuttage；Fine day is to greenhouse
Sterilizing is carried out, sprays anti-grass cloth and greenhouse film with 400 times of diluted fenaminosulf aqueous solutions, closing greenhouse close plastic shed is for 24 hours；Skewer
Greenhouse progress sterilizing is conducive to provide a less toxic growing environment for yulan before inserting breeding, while greenhouse facilitates control
The growth factors such as temperature, humidity, illuminance；
2）Preparing nursery bed：By garden mould, peat, perlite and pine needle layer according to 1:0.5: 0.3:0.5 ratio stirs evenly, dress
Pier is real after entering 50 hole seed plates of 10cm depths, and often 5 disk of row, is paved into seedbed, sprays, 1000 times of fenaminosulf solution sterilizings；Garden
Soil, rich in humus, probiotics, is suitably for newborn yulan seedling and provides sufficient nutrient, while peat and perlite can with pine needle layer
To be effectively improved the soil porosity and soil texture of garden mould and pine needle layer, make its close degree of having of pine, water holding getting fat；
3）Prepare cuttings：The branch for selecting no disease and pests harm, robust growth, neat and consistent, bud full, it is a length of in early morning clip cuttings
12cm, retains 3 buds, upper cut straight snips, and clip away from the 1st bud 2cm, at 0.8cm tiltedly cut below the section by lower cut；Cuttings is removed
It is soaked into 15s in 4 DEG C of preservation liquid outside bud-leaf, preserves the substance that liquid contains following content：3.0% glucan, 1.2% phosphoric acid
Potassium dihydrogen, the gibberellin of 15ppm, the brassin lactones of 20ppm, 0.3% single silicic acid, the ascorbic acid of 50ppm, 12ppm
(4S) -2,2- dimethyl -1,3-dioxolane -4- formaldehyde, surplus is water, and it is temporary that taking-up is placed on cold and moisture location；Early morning cuts
Take cuttings that can scatter and disappear to reduce cuttings excessive water to avoid high temperature and strong illumination, impregnate cuttings branch with cryopreservation solution
And cuttings transpiration dehydration can be effectively prevent in cold and moisture location is temporary, while can also facilitate and cuttings is managed concentratedly
Allotment；
4）Cuttage：It is as follows to be equipped with rooting agent solution：3.0% sucrose, the gibberellin of 25ppm, the aspirin of 15ppm, 30ppm
Ascorbic acid, 2.0% honey, the 6- benzyls aminoadenine of 40ppm, the heteroauxin of 60ppm, 8ppm (R)-(-) -2,2-
Diphenyl cyclopentanol, 0.6% urea, 0.4% potassium dihydrogen phosphate, 0.3% boric magnesium fertilizer, surplus is distilled water；It is moistened from cool place
Place takes out temporary cuttings, and by cuttings, entirety is soaked into 25s in rooting agent solution in addition to bud-leaf, by cuttings after standing 4s after taking-up
It is gently inserted into matrix with 70% length, per 1, cave cuttings, cuttings is inserted into hole simultaneously grave mound, fringe after the wooden stick hole pounding thicker than cuttings
Bud-leaf direction is unified, after a wheel fringe bar has been inserted, sprays immediately permeable, checks whether there is fringe bar and rush askew or blade face by water and be attached to base
In matter；Cutting medium can be effectively prevent to cause to scrape abrasion to cuttings to carry out cuttage after wooden stick hole pounding, be conducive to improve and insert
Fringe quality of rooting and quantity, after cuttings is dipped rooting agent solution, effective physiological compositions in rooting agent solution can pass through increasing
The plasticity that strong cell metabolism comes callus and skin zone's cell makes it add to promote this two-part cell growth, elongation
Fast-growing is long, breaks up, and finally grows root system, completes to take root；
5）It is managed after inserting：Water management, temperature management, sterilizing, the relevant management of fertilising are carried out after the completion of cuttage successively,
85% fringe bar can carry out hardening after taking root, and cuttage seeding is transferred to together with hole tray on overhead seedbed, pour 1 water every 4d, make
There is the state of semi-wet in matrix, opens greenhouse skylight, is stepped up ventilation time and shortens the shading time, improves Flos micheliae Albae
The adaptability of seedling, hardening 10d can be transplanted；The related managements such as good moisture, temperature, sterilizing, fertilising, no
Flos micheliae Albae can only be accelerated to take root growth, keep its root system healthy and strong, while additionally aiding the growth and development of seedling aerial part, accelerate
It is grown up, and by that can be transplanted after short-term hardening, cuttage survival rate is higher, and growth of seedling is healthy and strong.
Embodiment 3：
The propagation method of Flos micheliae Albae, including：Greenhouse pre-treatment, preparing nursery bed, prepare cuttings, cuttage, insert after manage, specifically include
Following steps：
Greenhouse pre-treatment：Greenhouse is cleared up before cuttage, removes the sundries such as moss, lichens, the residual matrix in anti-grass cloth；It is right
Skylight, shady net, wind turbine, wet curtain, control water system etc. are overhauled, and ensure greenhouse normal operation during cuttage；Fine day to greenhouse into
Row sterilizing sprays anti-grass cloth and greenhouse film, closing greenhouse close plastic shed 18h with 400 times of diluted fenaminosulf aqueous solutions；Cuttage
Sterilizing is carried out before breeding to greenhouse to be conducive to provide a less toxic growing environment for yulan, while greenhouse facilitates control temperature
The growth factors such as degree, humidity, illuminance；
Preparing nursery bed：By garden mould, peat, perlite and pine needle layer according to 1:0.4:0.3:0.5 ratio stirs evenly, and is packed into
Pier is real after 50 hole seed plates of 10cm depths, and often 5 disk of row, is paved into seedbed, sprays, 1000 times of fenaminosulf solution sterilizings；Garden mould
It with pine needle layer rich in humus, probiotics, is suitably for newborn yulan seedling and sufficient nutrient is provided, while peat and perlite can be with
It is effectively improved the soil porosity and soil texture of garden mould and pine needle layer, makes its close degree of having of pine, water holding getting fat；
Prepare cuttings：The branch for selecting no disease and pests harm, robust growth, neat and consistent, bud full, it is a length of in early morning clip cuttings
10cm, retains 3 buds, upper cut straight snips, and clip away from the 1st bud 2cm, at 0.6cm tiltedly cut below the section by lower cut；Cuttings is removed
12s in 2 DEG C of preservation liquid is soaked into outside bud-leaf, it is temporary that taking-up is placed on cold and moisture location；Early morning clip cuttings can be to avoid height
Mild strong illumination is scattered and disappeared with reducing cuttings excessive water, with cryopreservation solution immersion cuttings branch and temporary in cold and moisture location
Cuttings transpiration dehydration can be effectively prevent by depositing, while can also be facilitated and be carried out centralized management allotment to cuttings；
Cuttage：Temporary cuttings is taken out from cold and moisture location, by cuttings, entirety is soaked into 20s in rooting agent solution in addition to bud-leaf,
Cuttings is gently inserted into matrix with 2/3 length after standing 4s after taking-up, often 1, cave cuttings, to be incited somebody to action after the wooden stick hole pounding thicker than cuttings
Cuttings is inserted into hole and grave mound, fringe bar bud-leaf direction are unified, after a wheel fringe bar has been inserted, is sprayed immediately permeable, is checked whether there is fringe bar
Askew or blade face is rushed by water to be attached in matrix；Cutting medium can be effectively prevent to cause cuttings to carry out cuttage after wooden stick hole pounding
Abrasion is scraped, is conducive to improve cuttage rooting quality and quantity, after cuttings is dipped rooting agent solution, having in rooting agent solution
Imitating physiological compositions can be by enhancing cell metabolism come the plasticity of callus and skin zone's cell, to promote this two-part
Cell growth, elongation make its tachyauxesis, differentiation, finally grow root system, complete to take root；
It is managed after inserting：Water management, temperature management, sterilizing, the relevant management of fertilising are carried out after the completion of cuttage successively,
80% fringe bar can carry out hardening after taking root, and cuttage seeding is transferred to together with hole tray on overhead seedbed, pour 1 water every 3d, make
There is the state of semi-wet in matrix, opens greenhouse skylight, is stepped up ventilation time and shortens the shading time, improves Flos micheliae Albae
The adaptability of seedling, hardening 7d can be transplanted；The related managements such as good moisture, temperature, sterilizing, fertilising, no
Flos micheliae Albae can only be accelerated to take root growth, keep its root system healthy and strong, while additionally aiding the growth and development of seedling aerial part, accelerate
It is grown up, and by that can be transplanted after short-term hardening, cuttage survival rate is higher, and growth of seedling is healthy and strong.
Prepare the substance that the preservation liquid in cuttings step contains following content：2.8% glucan, 1% potassium dihydrogen phosphate,
(4S) -2 of the gibberellin of 12ppm, the brassin lactones of 20ppm, 0.25% single silicic acid, the ascorbic acid of 45ppm, 12ppm,
2- dimethyl -1,3-dioxolane -4- formaldehyde, surplus is water；A thin layer of aqueous solution can be formed on cuttings surface by preserving liquid
Film, has the function of moisturizing, fungi-proofing, and Flos micheliae Albae cuttings incision contains tannin, tannin be easily oxidized to form one layer it is fine and close
Isolation film, the isolation film can not only influence the entrance of subsequent growth element solution, can also cause to hinder to the newborn root system of callus
It scratches, reduces rooting rate and survival rate, the ascorbic acid preserved in liquid can seize oxidation material, prevent oxidation material to tannin
Excessive oxidation, meanwhile, water solubility can be formed by preserving (4S) -2,2- dimethyl -1,3-dioxolane -4- formaldehyde micro in liquid
Hydrate, the hydrate in the presence of single silicic acid can with tannin occur phenol-formaldehyde reaction, it is weak to reduce the oxidation of tannin
The thickness for changing the isolation film after tannin oxidation, does not interfere with callus and takes root, and improves survival rate.
Contain the substance of following content in rooting agent solution in cuttage step：3% sucrose, the gibberellin of 20ppm,
The aspirin of 14ppm, the ascorbic acid of 25ppm, 2% honey, the 6- benzyls aminoadenine of 35ppm, 55ppm heteroauxin,
(R)-(-) -2 of 6ppm, 2- diphenyl cyclopentanol, 0.55% urea, 0.3% potassium dihydrogen phosphate, 0.2% boric magnesium fertilizer, surplus
For distilled water；The initial stage of callus and Adventitious root initiation, cell it is rapid differentiation with growth make soluble sugar content drastically under
Drop, sucrose can provide required sugar with honey for root system, and the crescendo of root absorbability and photosynthesis are restored later, and sugar contains
Amount is gradually increasing again；Gibberellin, aspirin help to sterilize, and can prevent callus from rotting, prevent root system from infecting；6- benzyls
Aminoadenine, heteroauxin can act on cell, cambial cell can be stimulated to divide, and promote xylem, phloem cell
Differentiation promotes the morphogenesis for exceeding injured tissue differentiation, adjusting callus, hestening rooting；But heteroauxin easily aoxidized from
And its physiological function is lost, complex reaction can occur with heteroauxin for (R)-(-) -2,2- diphenyl cyclopentanol, and production is opposite
Stable complex compound loses adjustment effect so as to prevent heteroauxin from turning to indoles by photooxidation, which enters plant
Can be as a kind of storage form of heteroauxin in plant after in object, under proper condition, complex compound can be via solution
Complex reaction and be changed into sequestered, be transferred to site of action through transport and work；Heteroauxin and (R)-(-) -2,2- hexichol
The complex compound of basic ring amylalcohol may advantageously facilitate the secretion of superoxide dismutase in plant, and superoxide dismutase is to indefinite
The development of root plays a driving role, and can effectively facilitate the elongation and growth of adventitious root.
Water management is to ensure wet in cuttage greenhouse chamber by adjusting conditions such as spray time, interval time, ventilation situations
Degree is 95%, and a small amount of multiple principle is taken in spraying, has mist pearl without dripping for degree with cuttings surface；When cuttings starts to take root
Water spray number is suitably reduced, with sharp cuttage rooting, prevents root system from rotting.
Temperature management is by switching the facilities such as sunshade net, skylight, wet curtain, it is ensured that cuttage greenhouse indoor temperature is maintained at 30
DEG C, natural temperature is kept during hardening.
Sterilizing operates：Dusk after cuttage 14d closes greenhouse spraying, seedling is sprayed with 1500 times of carbendazim solutions
Bed, carry out disinfection sterilization, is carried out 1 time per 15d later；Sterilizing can sterilize, prevent disease from spreading, and reduce cuttings and rot
Rate reduces cuttings pest and disease damage infection rate, improves cuttage success rate and condition of rooting；
Fertilizing operation is：After cuttage 3 weeks, every 2 weeks, in dusk stop spraying, blade face moisture content dry after with 0.2% di(2-ethylhexyl)phosphate
Hydrogen potassium or 0.1% urea or nursery stock liquid fertilizer special carry out foliage dressing；Page fertilising relatively straightforward can provide foster for seedling
Point.
The survival rate of Flos micheliae Albae in Examples 1 to 3 and situation of taking root are made into statistics, statistical result is as shown in table 1.
The survival rate and situation of taking root of Flos micheliae Albae in 1. Examples 1 to 3 of table
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Cuttings survival rate（%） | 85.5 | 88.5 | 90.5 |
| Longest root long（cm） | 11.2 | 10.8 | 12.5 |
As shown in Table 1, in the method for the present invention, the survival rate of Flos micheliae Albae cuttings can reach in 85% or more, especially embodiment 3
Survival rate be more than 90%, and the length of the longest adventitious root in Examples 1 to 3 has been more than 10cm, illustrates present invention side
Method is a kind of propagation method of effective Flos micheliae Albae.
Routine operation in operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. the propagation method of Flos micheliae Albae, it is characterised in that：The propagation method of the Flos micheliae Albae includes：
Greenhouse pre-treatment：Fine day carries out sterilizing to greenhouse, and anti-grass is sprayed with 300~400 times of diluted fenaminosulf aqueous solutions
Cloth and greenhouse film, closing greenhouse close plastic shed 12~for 24 hours；
Preparing nursery bed：Garden mould, peat, perlite are uniformly mixed with pine needle layer, pier is real after being packed into 50 hole seed plates, often 5 disk of row
It is paved into seedbed, sprays 800~1000 times of fenaminosulf solution sterilizings；
Prepare cuttings：Cuttings is removed bud-leaf by the branch clip cuttings for selecting no disease and pests harm, robust growth, neat and consistent, bud full
10~15s in the outer preservation liquid for immersing 2~4 DEG C, it is temporary that taking-up is placed on cold and moisture location；
Cuttage：Temporary cuttings is taken out from cold and moisture location, cuttings is integrally soaked into except bud-leaf in addition to 20 in rooting agent solution~
Cuttings is gently inserted into matrix, will be inserted after the wooden stick hole pounding thicker than cuttings by 25s after 3~4s of standing after taking-up with 60~70% length
Fringe is inserted into hole and grave mound, after a wheel fringe bar has been inserted, sprays immediately permeable；
It is managed after inserting：To carry out water management, temperature management, sterilizing, the relevant management of fertilising after the completion of cuttage successively, 75
~85% fringe bar can carry out hardening after taking root, and 7~10d of hardening can be transplanted.
2. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：In the preparing nursery bed step, garden
Soil, peat, perlite and pine needle layer mixed proportion be 1:0.4~0.5:0.2~0.3:0.3~0.5.
3. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：In the preparation cuttings step, cuttings
A length of 8~12cm, retain 2~3 buds, upper cut straight snips, clip away from the 1st 1~2cm of bud, lower cut 0.5 below section~
It is tiltedly cut at 0.8cm.
4. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：In the preparation cuttings step, preserve
Liquid contains the substance of following content：2.5~3.0% glucan, 1.0~1.2% potassium dihydrogen phosphate, 12~15ppm it is red mould
Element, the brassin lactones of 15~20ppm, 0.2~0.3% single silicic acid, the ascorbic acid of 40~50ppm, 11~12ppm
(4S) -2,2- dimethyl -1,3-dioxolane -4- formaldehyde, surplus is water.
5. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：In the cuttage step, root-growing agent is molten
Contain the substance of following content in liquid：2.5~3.0% sucrose, the gibberellin of 20~25ppm, 12~15ppm aspirin,
The indoles second of the ascorbic acid of 20~30ppm, 1.6~2.0% honey, the 6- benzyls aminoadenine of 30~40ppm, 50~60ppm
Acid, (R)-(-) -2,2- diphenyl cyclopentanol of 5~8ppm, 0.5~0.6% urea, 0.3~0.4% potassium dihydrogen phosphate,
0.2~0.3% boric magnesium fertilizer, surplus are distilled water.
6. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：It is described to insert in rear management process, moisture
Management is to ensure that cuttage greenhouse indoor humidity is 90~95% by adjusting conditions such as spray time, interval time, ventilation situations,
A small amount of multiple principle is taken in spraying, has mist pearl without dripping for degree with cuttings surface.
7. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：It is described to insert in rear management process, temperature
Management is by switching the facilities such as sunshade net, skylight, wet curtain, it is ensured that and cuttage greenhouse indoor temperature is maintained between 27~32 DEG C,
Natural temperature is kept during hardening.
8. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：It is described to insert in rear management process, sterilization
Sterilizing operation is：Dusk after 12~14d of cuttage closes greenhouse spraying, seedbed is sprayed with 1000~1500 times of carbendazim solutions,
Carry out disinfection sterilization, and every 15~20d is carried out 1 time later.
9. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：It is described to insert in rear management process, fertilising
Operation is：After cuttage 3~4 weeks, every 2 weeks, in dusk stop spraying, blade face moisture content dry after with 0.2~0.3% di(2-ethylhexyl)phosphate
Hydrogen potassium or 0.1~0.2% urea or nursery stock liquid fertilizer special carry out foliage dressing.
10. the propagation method of Flos micheliae Albae according to claim 1, it is characterised in that：It is described to insert in rear management process, hardening
Operation is：Cuttage seeding is transferred to together with hole tray on overhead seedbed, 1 water is poured every 2~4d, matrix is made semi-wet occur
State opens greenhouse skylight, is stepped up ventilation time and shortens the shading time.
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Application publication date: 20181113 |