CN108732163B - Full-automatic chemiluminescence immunoassay instrument - Google Patents
Full-automatic chemiluminescence immunoassay instrument Download PDFInfo
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- CN108732163B CN108732163B CN201810820822.2A CN201810820822A CN108732163B CN 108732163 B CN108732163 B CN 108732163B CN 201810820822 A CN201810820822 A CN 201810820822A CN 108732163 B CN108732163 B CN 108732163B
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- 238000003018 immunoassay Methods 0.000 title claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 119
- 238000004140 cleaning Methods 0.000 claims abstract description 100
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 85
- 238000002156 mixing Methods 0.000 claims abstract description 80
- 238000001514 detection method Methods 0.000 claims abstract description 50
- 230000003287 optical effect Effects 0.000 claims abstract description 30
- 238000005086 pumping Methods 0.000 claims abstract description 25
- 230000005540 biological transmission Effects 0.000 claims description 26
- 230000007246 mechanism Effects 0.000 claims description 25
- 239000000758 substrate Substances 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 21
- 238000003825 pressing Methods 0.000 claims description 13
- 238000005096 rolling process Methods 0.000 claims description 3
- 230000003749 cleanliness Effects 0.000 abstract description 4
- 238000002347 injection Methods 0.000 description 20
- 239000007924 injection Substances 0.000 description 20
- 230000009471 action Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 238000005406 washing Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000037452 priming Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 230000005291 magnetic effect Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000011324 bead Substances 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005294 ferromagnetic effect Effects 0.000 description 2
- 238000000504 luminescence detection Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a full-automatic chemiluminescence immunoassay instrument, and relates to the technical field of medical instruments. The analyzer comprises a base, wherein a cleaning and mixing device and an optical detection device are arranged on the base, the cleaning and mixing device comprises a cleaning disc, a first driving device for driving the cleaning disc to rotate, a liquid pumping device, a liquid injecting device and a mixing device which are positioned above the cleaning disc, and a lifting device for driving the liquid injecting device, the liquid pumping device and the mixing device to move up and down in the vertical direction is also arranged on the base; the mixing device comprises a gripper for gripping the reagent tube and a second driving device for driving the gripper to rotate; the upper end face of the cleaning disc is provided with evenly distributed tube placing holes, and the outer edge of the cleaning disc is provided with optical detection holes communicated with the tube placing holes; the optical detection device comprises a photon counter arranged on the base and close to the outer edge of the cleaning disc. The invention can improve the cleaning cleanliness and the cleaning efficiency, and can also improve the detection efficiency and the detection accuracy.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a full-automatic chemiluminescence immunoassay instrument.
Background
The chemiluminescent immunoassay technology (Chemiluminescence immunoassay, abbreviated as CLIA) is a non-radioactive immunoassay technology which is developed very rapidly worldwide at present, has the advantages of high sensitivity, strong specificity, wide linear range, high automation degree and the like, and becomes a main means of clinical diagnosis.
Most of the full-automatic chemiluminescence analyzers in the current market generally consist of a mechanical arm or a chain transmission mechanism, a reagent sample part, an incubation part, a cleaning part, a luminescence detection structure and the like. In the detection process, generally, a reagent tube is added with a sample, magnetic beads and a reagent, after incubation is completed, a sample reaction tube is clamped and transferred to a cleaning position by a manipulator for magnetic separation, cleaning, uniform mixing and luminescent substrate injection, and then transferred to a luminescent detection position by a transmission mechanism for detection. The cleaning and mixing process of the reagent tube has the most direct influence on the luminous effect, and the incomplete cleaning and mixing often causes the problems of unstable luminous effect, distorted detection result and the like. In one detection process, a manipulator is required to grasp the reaction tube for multiple transfer operations, and the problems of excessive temperature and energy loss, incomplete reaction and the like often exist in the transfer process of the sample reaction tube, and even the optimal luminescence detection time can be missed, so that the problems of larger error of a test result, unstable detection result and the like are caused; in addition, excessive grabbing and placing actions of the reaction tube easily cause the problems of reduced testing reliability and testing efficiency.
Disclosure of Invention
The invention provides a full-automatic chemiluminescence immunoassay analyzer which can improve the cleaning cleanliness and the cleaning efficiency and can also improve the detection efficiency and the detection accuracy.
In order to solve the technical problems, the invention provides the following technical scheme:
the utility model provides a full automation luminous immunoassay appearance, includes the base, be provided with on the base and wash mixing device and optical detection device, wherein:
the cleaning and mixing device comprises a cleaning disc arranged on the base, a first driving device for driving the cleaning disc to rotate, and a liquid pumping device, a liquid injecting device and a mixing device which are arranged above the cleaning disc, wherein the base is also provided with a lifting device for driving the liquid injecting device, the liquid pumping device and the mixing device to move up and down in the vertical direction;
the liquid pumping device, the liquid injecting device and the mixing device are respectively at least two;
the mixing device comprises a gripper for gripping the reagent tube and a second driving device for driving the gripper to rotate;
The upper end face of the cleaning disc is provided with evenly distributed tube placing holes, and the outer edge of the cleaning disc is provided with optical detection holes communicated with the tube placing holes;
The optical detection device comprises a photon counter which is arranged on the base and is close to the outer edge of the cleaning disc.
Further, the lifting device comprises a first spiral lifter arranged on the base and a first lifting platform arranged at the output end of the first spiral lifter, the first lifting platform is located above the cleaning disc, and the liquid pumping device, the liquid injecting device and the mixing device are all arranged on the first lifting platform.
Further, a first guiding device is further arranged between the first spiral lifter and the first lifting platform, and the first guiding device adopts a guide rail sliding block transmission mechanism, a worm gear transmission mechanism or a gear rack transmission mechanism.
Further, the base is provided with a rotating shaft fixedly connected with the gripper, the rotating shaft is connected with the base through a rolling bearing, the second driving device comprises a first motor arranged on the first lifting platform, a first driving wheel arranged at the output end of the first motor and a first driven wheel fixedly connected with the rotating shaft, and the first driving wheel is connected with the first driven wheel through a first transmission belt.
Further, the grip is made of an elastic sleeve, and open grooves communicated with the inner wall of the sleeve are uniformly formed in the outer edge of the lower end of the sleeve.
Further, the bottom end of the sleeve is provided with a conical opening.
Further, the terminal surface of pivot is provided with first through-hole, be provided with in the first through-hole with pivot clearance fit's top pipe box, still be provided with on the first lift platform and press the pipe device, press the pipe device including set up in second spiral lift on the first lift platform and with the second lift platform that the output of second lift is connected, be provided with on the second lift platform and be located the pipe pressing piece of top pipe box top.
Further, the grip and the liquid injection needle of the liquid injection device are coaxially arranged, and the liquid injection needle penetrates through the inner hole of the top pipe sleeve.
Further, a second guiding device is arranged between the first lifting platform and the second lifting platform;
the second guide device comprises a guide rod, and a guide sleeve in clearance fit with the guide rod is arranged on the second lifting platform;
Or the second guiding device adopts a guide rail sliding block transmission mechanism, a worm and gear transmission mechanism or a gear and rack transmission mechanism.
Further, an electrothermal film is arranged on the inner wall of the cleaning disc, and a pipeline heating structure for heating cleaning liquid and a luminous substrate heating structure for heating luminous substrates are arranged on the base.
Compared with the prior art, the invention has the following beneficial effects:
The full-automatic chemiluminescence immunoassay analyzer is characterized in that at least two liquid pumping devices, liquid injecting devices and mixing devices are respectively arranged above a cleaning disc, and an optical detection device is arranged at a position close to the outer edge of the cleaning disc. One of the liquid injection device and the mixing device is used for injecting and mixing substrates, and the other liquid extraction device, the liquid injection device and the mixing device are used for cleaning the reagent tube. The first driving device can control the cleaning disc to rotate at a certain angle so that the reagent tube in the cleaning disc reaches the lower part of the corresponding liquid pumping device and the liquid injecting device (mixing device) to clean and mix uniformly, and the substrate is injected and mixed uniformly below the last liquid injecting device. Finally, the first driving device controls the cleaning disc to rotate by a certain angle so that the reagent tube in the cleaning disc reaches the position corresponding to the optical detection device, and the optical detection device detects the reactant in the reagent tube through the optical detection hole on the outer edge of the cleaning disc. According to the full-automatic chemiluminescence immunoassay analyzer, the mixing device is arranged, the grip of the mixing device is coaxially arranged with the liquid injection needle of the liquid injection device, and the rotating mixing step for the reagent tube is added when the reagent tube is cleaned, so that the cleaning cleanliness and the cleaning efficiency of the reagent tube are improved. In addition, the optical detection device is arranged at a position close to the outer edge of the cleaning disc, the optical detection hole communicated with the tube placing hole is arranged on the outer edge of the cleaning disc, the reagent tube in the reagent disc can reach the optical detection position only by rotating a certain angle under the control of the first driving device, and the photon counter of the optical detection device directly carries out photon reading detection based on the optical detection hole on the cleaning disc, so that the energy consumption and the transfer error generated by the movable type of the reagent tube after substrate filling are avoided, the risk of cross contamination is avoided, the photon reading error is reduced, the detection efficiency and the detection accuracy are improved, and the reliability of the detection result is ensured.
Drawings
FIG. 1 is a schematic diagram of the structure of a full-automatic chemiluminescence immunoassay analyzer of the present invention;
FIG. 2 is a schematic diagram showing the bottom structure of the full-automatic chemiluminescence immunoassay of the present invention;
FIG. 3 is a schematic diagram of a washing and mixing device in the full-automatic chemiluminescence immunoassay analyzer;
fig. 4 is a schematic structural diagram of a mixing device in the full-automatic chemiluminescence immunoassay analyzer.
Detailed Description
In order to make the technical problems, technical solutions and advantages to be solved more apparent, the following detailed description will be given with reference to the accompanying drawings and specific embodiments.
The invention provides a full-automatic chemiluminescence immunoassay analyzer, as shown in fig. 1 to 4, which comprises a base 1, wherein a cleaning and mixing device and an optical detection device 10 are arranged on the base, and the full-automatic chemiluminescence immunoassay analyzer comprises the following components:
The cleaning and mixing device comprises a cleaning disc 2 arranged on a base 1, a first driving device 3 for driving the cleaning disc 2 to rotate, a liquid pumping device 6, a liquid injecting device 5 and a mixing device 4 which are positioned above the cleaning disc 2, and a lifting device 7 for driving the liquid injecting device 5, the liquid pumping device 6 and the mixing device 4 to move up and down in the vertical direction is also arranged on the base 1;
the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 are respectively at least two;
The mixing device 4 comprises a grip 4-1 for gripping the reagent tube and a second driving device 4-2 for driving the grip 4-1 to rotate;
the upper end face of the cleaning disc 2 is provided with evenly distributed tube placing holes 2-1, and the outer edge of the cleaning disc 2 is provided with optical detection holes 2-2 communicated with the tube placing holes 2-1;
The optical detection means 10 comprise a photon counter 10-1 arranged on the base 1 and close to the outer edge of the washing disc 2.
In the full-automatic chemiluminescence immunoassay analyzer, when in an initial position, the lifting device 7 controls the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 to lift so as to be completely positioned above the cleaning disc 2. After the reagent tube after incubation is put into the cleaning disc 2 through the manipulator, the first driving device 3 controls the cleaning disc 2 to rotate a certain angle so that the reagent tube reaches the lower part of the liquid extracting device 6, at the moment, the lifting device 7 controls the liquid extracting device 6 to descend so that waste liquid in the reagent tube is extracted, then the lifting device 7 controls the liquid extracting device 6 to ascend, the first driving device 3 controls the cleaning disc 2 to rotate a certain angle again so that the reagent tube reaches the lower part of the liquid injecting device 5 and the mixing device 4, at the moment, the lifting device 7 controls the liquid injecting device 5 to inject cleaning liquid into the reagent tube, the gripper 4-1 of the mixing device 4 grabs the reagent tube, the lifting device 7 controls the liquid injecting device 5 and the mixing device 4 to ascend so that the reagent tube is separated from the tube hole 2-1 of the cleaning disc 2, and the driving device controls the gripper 4-1 to rotate under the grabbing action of the gripper 4-1, so that the mixing cleaning work in the reagent tube is completed, and the cleaning degree of the reagent tube is improved. After the reagent tube is washed, the first driving device 3 drives the washing disc 2 to rotate a certain angle so that the reagent tube reaches the lower part of the other liquid extracting device 6, the lifting mechanism drives the liquid extracting device 6 to descend to extract waste liquid in the reagent tube, then the lifting mechanism drives the liquid extracting device 6 to ascend, the first driving device 3 drives the washing disc 2 to rotate a certain angle so that the reagent tube reaches the lower part of the other liquid injecting device 5, the lifting mechanism drives the liquid injecting device 5 to descend, the substrate is injected into the reagent tube by the liquid injecting device 5, the gripper 4-1 of the mixing device 4 grabs the reagent tube, then the lifting device 7 controls the liquid injecting device 5 and the mixing device 4 to ascend so that the reagent tube is separated from the tube hole 2-1 of the washing disc 2, the driving device controls the gripper 4-1 to rotate, and the reagent tube rotates under the grabbing action of the gripper 4-1, so that the mixing operation of the substrate in the reagent tube is completed. The first driving device 3 drives the cleaning disc 2 to rotate a certain angle so that the reagent tube reaches the position corresponding to the optical detection device 10, and the optical detection device 10 detects the reactant in the reagent tube.
In summary, the full-automatic chemiluminescence immunoassay analyzer is characterized in that the mixing device 4 is arranged, the grip 4-1 of the mixing device 4 is coaxially arranged with the liquid injection needle of the liquid injection device 5, and after the liquid injection device 5 injects the cleaning liquid and the luminescent substrate into the reagent tube, the mixing device 4 rotates the reagent tube, so that the cleaning liquid can sufficiently clean the reagent tube, the cleaning of the reagent tube is more thorough, and the cleaning efficiency is also improved; and the chemical reaction of the luminescent substrate in the reagent tube is accelerated by the mixing function of the mixing device 4. In addition, the optical detection device 10 is arranged at a position close to the outer edge of the cleaning disc 2, the first driving device 3 controls the cleaning disc 2 to rotate for a certain angle, then the reagent tube with luminescent substrates added into the cleaning disc 2 can be transferred to an optical detection position, the photon counter 10-1 of the optical detection device 10 can count and detect the luminescent substrates in the reagent tube through the optical detection hole 2-2 of the cleaning disc 2, so that the distance that the luminescent substrates are added into the reagent tube and then transferred to the optical detection position is reduced, energy consumption generated in the transferring processes of grabbing, placing and the like of the reagent tube is avoided, the detection efficiency is improved, the error of photon reading is reduced, and the detection result is ensured.
In an embodiment of the present invention, the first driving device 3 may drive the cleaning disc 2 to rotate by adopting a motor to drive a driving belt, specifically, as shown in fig. 2, the first driving device 3 may include a first motor 3-1 fixedly disposed on the base 1, a first driving pulley 3-2 fixedly connected to an output end of the first motor 3-1, and a first driven pulley 3-4 fixedly connected to the cleaning disc 2, where the first driving pulley 3-2 and the first driven pulley 3-4 are connected by adopting the first driving belt 3-3. The first drive belt 3-3 can be designed in the form of a synchronous belt in order to increase the transmission accuracy of the first drive 3.
Of course, the first driving device 3 can adopt a structure mode of motor driving transmission belt, and can adopt a structure mode of motor driving gear meshing to realize the rotation control of the cleaning disc 2.
In addition, before the reagent vessel after incubation is placed in the washing tray 2, it is necessary to control a certain tube placing hole 2-1 of the washing tray 2 to a specified position so that the reagent vessel is placed in the tube placing hole 2-1. In determining whether the discharge hole 2-1 reaches a specified position, a structure in which the first photoelectric switch 3-5 and the first photoelectric switch shutter 3-6 are provided on the base 1 and the first driven pulley 3-4 may be adopted. When a reagent tube needs to be placed in the tube placing hole 2-1, the first motor 3-1 controls the first driving belt pulley 3-2 to rotate, and therefore the cleaning disc 2 is driven to rotate through the first driven belt pulley 3-4, when the first photoelectric switch 3-5 enters the sensing position of the first photoelectric switch baffle plate 3-6, the first photoelectric switch 3-5 sends the signal to a control system of the full-automatic chemiluminescence immunoassay instrument, the control system controls the first motor 3-1 to stop rotating according to the signal, the tube placing hole 2-1 can reach a designated position, and then other mechanisms of the full-automatic chemiluminescence immunoassay instrument can place the reagent tube in the tube placing hole 2-1 for subsequent cleaning and detection.
In an embodiment of the present invention, in addition to the photon counter 10-1, the optical detection device 10 may be provided with a light shielding plate 10-2 on two sides of the photon counter 10-1 to shield the light source in order to reduce the influence of the external light source on the luminescent substrate in the reagent tube.
In an embodiment of the present invention, the lifting device 7 may adopt a structure of a screw lifter to control lifting of the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 relative to the cleaning disc 2. Specifically, the lifting device 7 preferably includes a first spiral lifter 7-1 disposed on the base 1 and a first lifting platform 7-2 disposed at an output end of the first spiral lifter 7-1, where the first lifting platform 7-2 is located above the cleaning disc 2, and the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 are all disposed on the first lifting platform 7-2. When the first spiral lifter 7-1 is electrified to rotate and lift, the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 are controlled to lift simultaneously through the first lifting platform 7-2.
The liquid extracting device 6 may include a liquid extracting needle 6-1 and a liquid extracting needle support 6-2 fixedly connected to the first lifting platform 7-2, where the liquid extracting needle 6-1 is fixed to the liquid extracting needle support 6-2 through a fastening screw. When the first lifting platform 7-2 is lifted under the control of the first spiral lifter 7-1, the liquid sucking needle 6-1 can lift relative to the reagent tube in the cleaning disc 2 to perform liquid sucking action.
The priming device 5 may also include a priming needle 5-1 and a priming needle holder 5-2 fixedly connected to the first lifting platform 7-2, the priming needle 5-1 being fixed to the priming needle holder 5-2. When the first lifting platform 7-2 is lifted under the control of the first spiral lifter 7-1, the liquid injection needle 5-1 can lift relative to the reagent tube in the cleaning disc 2 to perform liquid injection action.
In one embodiment of the present invention, the number of the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 is three, the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 are annularly arranged on the first lifting platform 7-2, and a set of mixing structure is formed by one liquid pumping device 6, one liquid injecting device 5 and one mixing device 4, wherein the two sets of mixing structures are used for cleaning the reagent tube, and the last set of mixing structure is used for injecting and mixing the luminous substrate. According to the invention, by arranging a plurality of sets of mixing structures and cleaning the reagent tube for a plurality of times, the cleaning cleanliness of the reagent tube is further improved, luminescent substrates are directly injected into the reagent tube in the cleaning disc 2 after the reagent tube is cleaned, so that cross contamination of the reagent tube in the grabbing and transferring processes is avoided, and the detection precision is improved.
In an embodiment of the present invention, a plurality of ferromagnetic devices 9 are further disposed on the base 1 near the outer edge of the cleaning disc 2, and each of the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 corresponds to one ferromagnetic device 9. When the cleaning disc 2 rotates for a certain angle to transfer the reagent tube to the lower parts of the liquid extracting device 6, the liquid injecting device 5 and the mixing device 4, the magnetic beads in the reagent tube are adsorbed on the inner wall of the reagent tube based on the magnetism of the strong magnetic device 9, so that the liquid extracting needle 6-1 of the liquid extracting device 6 can not extract the magnetic beads out of the reagent tube during liquid extracting.
Preferably, in order to improve the stability of the first lifting platform 7-2 in the lifting process, a first guiding device 7-3 may be further disposed between the first spiral lifter 7-1 and the first lifting platform 7-2, and the first guiding device 7-3 may adopt any structural form of a guide rail sliding block transmission mechanism, a worm gear transmission mechanism and a rack and pinion transmission mechanism, so that the guiding function on the first lifting platform 7-2 can be achieved.
When the first guiding means 7-3 takes the form of a rail-slider transmission, the first guiding means 7-3 preferably comprises a rail provided on the base 1 and a slider provided at the output end of the first screw lift 7-1 and slidably connected to the rail.
In addition, when the liquid drawing device 6, the liquid injecting device 5 and the mixing device 4 descend under the control of the first screw lifter 7-1, in order to prevent damage to the reagent tube caused by excessively low descending heights of the liquid drawing needle 6-1, the liquid injecting needle 5-1 and the gripper 4-1, a second photoelectric switch 7-5 and a second photoelectric switch baffle 7-4 can be respectively arranged on the guide rail and the slide block. When the first spiral lifter 7-1 drives the liquid drawing device 6, the liquid injection device 5 and the mixing device 4 to descend so that the liquid drawing needle 6-1, the liquid injection needle 5-1 and the grabbing hand 4-1 reach proper heights, the second photoelectric switch 7-5 enters the sensing position of the second photoelectric switch baffle 7-4, the control system of the full-automatic luminous immunoassay analyzer controls the first spiral lifter 7-1 to stop descending according to the received signals of the second photoelectric switch 7-5, and then the liquid drawing device 6, the liquid injection device 5 and the mixing device 4 perform corresponding liquid drawing, liquid injection and mixing actions.
In an embodiment of the present invention, the liquid pumping device 6, the liquid injecting device 5 and the mixing device 4 are sequentially and fixedly connected to the first lifting platform 7-2, wherein the liquid injecting needle 5-1 and the gripper 4-1 can respectively correspond to the two pipe holes 2-1, after the liquid injecting needle 5-1 injects liquid into the reagent pipe, the lifting device 7 controls the liquid injecting device 5 to lift, then the first driving device 3 controls the cleaning disc 2 to rotate a certain angle to enable the reagent pipe to reach the lower side of the gripper 4-1, and then the lifting device 7 controls the mixing device 4 to descend, and the gripper 4-1 grips the reagent pipe to perform the mixing action.
In addition to the above-described structure, the injection needle 5-1 and the grip 4-1 may be coaxially disposed so as to correspond to the same discharge hole 2-1. Specifically, the grip 4-1 may be connected to the base 1 through the rotation shaft 4-3, and the rotation shaft 4-3 is connected to the base 1 through the rolling bearing 4-4, thereby ensuring that the grip 4-1 can rotate relative to its axis. At this time, the end surface of the rotating shaft 4-3 needs to be provided with a first through hole for passing through the liquid injection needle 5-1 of the liquid injection device 5, the second driving device 4-2 can also control the rotation of the grip 4-1 by adopting a motor driving belt wheel structure mode, specifically, the second driving device 4-2 can comprise a second motor 4-2-1 arranged on the first lifting platform 7-2, a second driving wheel 4-2-2 connected with the output end of the second motor 4-2-1 and a second driven wheel 4-2-4 fixedly connected with the rotating shaft 4-3, and the second driving wheel 4-2-2 is connected with the second driven wheel 4-2-4 by adopting a second driving belt 4-2-3.
In addition, since the cleaning tray 2 has a plurality of reagent tubes and a mixing mechanism, a plurality of reagent tubes can be cleaned and mixed at the same time. In order to reduce the cost, the two adjacent turning hands can be driven by one second motor 4-2-1, specifically, the second driving belt 4-2-3 can be connected with the second driving wheel 4-2-2 and the two second driven wheels 4-2-4 at the same time, and after the second motor 4-2-1 starts to work, the second driving wheel 4-2-2 drives the two second driven wheels 4-2-4 to rotate by driving the second driving belt 4-2-3, so that the two grippers 4-1 rotate at the same time.
Further, the grip 4-1 is preferably made of an elastic sleeve, and the outer edge of the lower end of the sleeve is uniformly provided with open grooves 4-1-1 communicated with the inner wall of the sleeve. When the grip 4-1 descends under the control of the lifting device 7, the upper end of the reagent vessel can be directly inserted into the inner wall of the casing due to the elastic action of the casing and the arrangement of the open slot 4-1-1, and then fixed in the inner hole of the casing.
Preferably, when the lifting device 7 drives the grip 4-1 to descend, in order to enable the upper end of the reagent tube to smoothly enter the inner hole of the sleeve, the bottom end of the sleeve can be provided with a conical opening, and the diameter of the lower end of the conical opening is larger than that of the inner hole of the sleeve.
After the mixing device 4 mixes the liquid in the reagent tube, the lifting device 7 controls the grip 4-1 to descend to put the reagent tube back to the tube hole 2-1, and as the grip 4-1 cannot independently complete the function of putting the reagent tube back to the tube hole 2-1, the tube pressing device 11 is required to assist in matching to take the reagent tube out of the grip 4-1. Specifically, a pipe jacking sleeve 4-5 in clearance fit with the rotating shaft 4-3 may be disposed in the first through hole of the rotating shaft 4-3, the pipe pressing device 11 is disposed on the first lifting platform 7-2, the pipe pressing device 11 preferably includes a second spiral lifter 11-1 disposed on the first lifting platform 7-2 and a second lifting platform 11-2 connected with an output end of the second spiral lifter 11-1, and the pipe pressing piece 11-3 located above the pipe jacking sleeve 4-5 is disposed on the second lifting platform 11-2. When the second spiral lifter 11-1 controls the second lifting platform 11-2 to drive the pipe pressing piece 11-3 to descend, the pipe pressing piece 11-3 contacts with the upper end of the pipe jacking sleeve 4-5 and pushes the pipe jacking sleeve 4-5 to move downwards, after the pipe jacking sleeve 4-5 moves downwards for a certain distance, the lower end of the pipe jacking sleeve 4-5 contacts with the upper end of the reagent pipe, and when the pipe pressing piece 11-3 continues to move downwards, the reagent pipe withdraws from the inner hole of the sleeve to fall to the pipe placing hole 2-1 under the pushing action of the pipe jacking sleeve 4-5, and then the gripper 4-1 ascends under the driving of the lifting device 7, so that the placement of the reagent pipe is completed.
In addition, the tube pressing device 11 may further include a third photoelectric switch baffle 11-6 and a third photoelectric switch baffle 11-6 respectively disposed on the first lifting platform 7-2 and the second lifting platform 11-2, and when the second screw lifter 11-1 controls the second lifting platform 11-2 to descend to a certain height relative to the first lifting platform 7-2, the third photoelectric switch baffle 11-6 enters the sensing position of the third photoelectric switch baffle, and the control system of the full-automatic luminescence immunoassay analyzer controls the second screw lifter 11-1 to stop descending according to the received signal of the third photoelectric switch baffle 11-6, so that the tube pressing piece 11-3 can be prevented from crushing the reagent tube due to the excessively low descending height.
Similarly, when the second spiral lifter 11-1 controls the second lifting platform 11-2 to lift, in order to improve the stability of the second lifting platform 11-2 in the lifting process, a second guiding device 11-4 is preferably arranged between the second spiral lifter 11-1 and the second lifting platform 11-2, and the second guiding device 11-4 can adopt any structural form of a guide rail sliding block transmission mechanism, a worm gear transmission mechanism and a gear rack transmission mechanism, so that the guiding function of the second lifting platform 11-2 can be realized.
Of course, as shown in fig. 3, the second guiding device 11-4 may also adopt a structure mode of matching a guiding rod and a guiding sleeve, wherein the first lifting platform 7-2 may be provided with the guiding sleeve, and the second lifting platform 11-2 is provided with the guiding rod in clearance fit with the guiding sleeve.
In an embodiment of the present invention, in order to avoid the influence of temperature variation during the movement of the reagent tube in the cleaning disc 2, and further to cause inaccuracy of the detection result, an electrothermal film such as PI electrothermal film may be disposed on the inner wall of the cleaning disc 2, and the disposition of the electrothermal film may be used to simulate the human body temperature, so as to ensure that the reagent tube is not influenced by temperature variation.
Furthermore, a pipe heating structure 8 for heating the cleaning liquid and a luminous substrate heating structure for heating the substrate may be provided on the base 1. The pipeline heating structure 8 and the luminous substrate heating structure are the same and comprise a heating inner core and a heating outer wall, the heating inner core is used for fixing a pipeline and conducting heat, and the heating outer wall is provided with an electrothermal film and is wrapped with heat preservation cotton. The pipeline heating structure 8 and the luminous substrate heating structure are respectively fixed on the base 1 through end covers made of POM materials, so that the heat insulation performance is ensured, and the heating efficiency is improved.
In summary, the full-automatic chemiluminescence immunoassay analyzer integrates three functions of multi-step cleaning, uniform mixing and optical detection into an integrated system, so that the structural complexity of the analyzer is reduced, the structural reliability is improved, and the overall manufacturing cost of the analyzer is reduced; the multi-step cleaning and mixing mode ensures the cleaning effect, can simultaneously clean and mix a plurality of reagent tubes, and improves the cleaning efficiency and the reaction speed of luminous substrates; the structural form of the integrated system avoids the transfer error and energy consumption of the reagent tube in the transfer process, has no cross contamination, reduces photon reading error, improves test efficiency and ensures the reliability of the detection result.
While the foregoing is directed to the preferred embodiments of the present invention, it will be appreciated by those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present invention, and such modifications and adaptations are intended to be comprehended within the scope of the present invention.
Claims (7)
1. The utility model provides a full automation luminous immunoassay appearance, includes the base, its characterized in that is provided with on the base and washs mixing arrangement and optical detection device, wherein: the cleaning and mixing device comprises a cleaning disc arranged on the base, a first driving device for driving the cleaning disc to rotate, and a liquid pumping device, a liquid injecting device and a mixing device which are arranged above the cleaning disc, wherein the base is also provided with a lifting device for driving the liquid injecting device, the liquid pumping device and the mixing device to move up and down in the vertical direction;
The first driving device comprises a first motor fixedly arranged on the base, a first driving belt pulley fixedly connected with the output end of the first motor and a first driven belt pulley fixedly connected with the cleaning disc, and the first driving belt pulley is connected with the first driven belt pulley by a first transmission belt;
the mixing device comprises a gripper for gripping the reagent tube and a second driving device for driving the gripper to rotate;
The upper end face of the cleaning disc is provided with evenly distributed tube placing holes, and the outer edge of the cleaning disc is provided with optical detection holes communicated with the tube placing holes;
A first photoelectric switch is arranged on the first driven belt pulley, and a first photoelectric switch baffle is arranged on the base;
The liquid pumping device, the liquid injecting device and the mixing device are respectively three in number and are annularly arranged on the first lifting platform, one liquid pumping device, one liquid injecting device and one mixing device form a set of mixing structure, wherein the two sets of mixing structures are used for cleaning a reagent tube, and the last set of mixing structure is used for injecting and mixing luminous substrates;
The optical detection device comprises a photon counter which is arranged on the base and is close to the outer edge of the cleaning disc;
The base is provided with a rotating shaft fixedly connected with the gripper, the rotating shaft is connected with the base through a rolling bearing, the second driving device comprises a first motor arranged on the first lifting platform, a first driving wheel arranged at the output end of the first motor and a first driven wheel fixedly connected with the rotating shaft, and the first driving wheel is connected with the first driven wheel through a first transmission belt; the end face of the rotating shaft is provided with a first through hole, a top pipe sleeve in clearance fit with the rotating shaft is arranged in the first through hole, a pipe pressing device is further arranged on the first lifting platform, the pipe pressing device comprises a second spiral lifter arranged on the first lifting platform and a second lifting platform connected with the output end of the second spiral lifter, and a pipe pressing piece positioned above the top pipe sleeve is arranged on the second lifting platform; the tongs and the annotate liquid needle of annotating the liquid device coaxial setting, annotate liquid needle and wear to locate the hole of top pipe box.
2. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein the lifting device comprises a first spiral lifter arranged on the base and a first lifting platform arranged at an output end of the first spiral lifter, the first lifting platform is located above the cleaning tray, and the liquid pumping device, the liquid injecting device and the mixing device are all arranged on the first lifting platform.
3. The full-automatic chemiluminescence immunoassay analyzer of claim 2, wherein a first guiding device is further arranged between the first spiral lifter and the first lifting platform, and the first guiding device adopts a guide rail sliding block transmission mechanism, a worm gear transmission mechanism or a gear rack transmission mechanism.
4. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein the grip is made of a sleeve with elasticity, and the outer edge of the lower end of the sleeve is uniformly provided with an opening groove communicated with the inner wall of the sleeve.
5. The full-automatic chemiluminescence immunoassay analyzer of claim 4, wherein the bottom end of the sleeve is provided with a tapered mouth.
6. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein a second guiding device is arranged between the first lifting platform and the second lifting platform;
the second guide device comprises a guide rod, and a guide sleeve in clearance fit with the guide rod is arranged on the second lifting platform;
Or the second guiding device adopts a guide rail sliding block transmission mechanism, a worm and gear transmission mechanism or a gear and rack transmission mechanism.
7. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein an electrothermal film is arranged on the inner wall of the cleaning tray, and a pipeline heating structure for heating the cleaning liquid and a luminous substrate heating structure for heating the luminous substrate are arranged on the base.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109596528B (en) * | 2018-12-11 | 2021-12-31 | 迪瑞医疗科技股份有限公司 | Full-automatic coagulation analyzer and analysis method thereof |
| CN109675890B (en) * | 2019-02-20 | 2024-04-02 | 重庆科斯迈生物科技有限公司 | Disk type chemiluminescent immunoassay analyzer washing system |
| CN110801763A (en) * | 2019-11-09 | 2020-02-18 | 山东博科生物产业有限公司 | Reaction tube grabbing and mixing device and analyzer |
| CN114082734A (en) * | 2021-09-10 | 2022-02-25 | 北京胡曼智造科技有限责任公司 | Cleaning system |
| CN114295853B (en) * | 2021-12-13 | 2024-08-20 | 杭州睿丽科技有限公司 | Full-automatic chemiluminescence immunoassay analyzer and use method thereof |
| CN116818686A (en) * | 2022-12-16 | 2023-09-29 | 广东牧玛生命科技有限公司 | POCT biochemical analyzer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105445258A (en) * | 2016-01-04 | 2016-03-30 | 泰州泽成生物技术有限公司 | Integrated cleaning and detecting device for chemiluminescence instrument |
| CN207181272U (en) * | 2017-09-28 | 2018-04-03 | 深圳市锦瑞生物科技有限公司 | Magnetic bead cleans separator and immunity analysis instrument automatically |
| CN208588675U (en) * | 2018-07-24 | 2019-03-08 | 山东博科诊断科技有限公司 | Full-automatic chemiluminescence immunoassay analysis meter |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102998473B (en) * | 2012-12-19 | 2014-06-11 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer |
| CN103592450B (en) * | 2013-11-28 | 2015-03-11 | 博奥赛斯（天津）生物科技有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
| CN204203242U (en) * | 2014-09-30 | 2015-03-11 | 博奥赛斯（天津）生物科技有限公司 | A kind of automatic chemiluminescence immunoassay device |
| CN205301342U (en) * | 2015-12-23 | 2016-06-08 | 深圳雷杜生命科学股份有限公司 | Automatic washing, mixing and separator |
| CN105628687B (en) * | 2016-02-24 | 2018-08-07 | 南京诺尔曼生物技术有限公司 | A kind of Full-automatic chemiluminescence analyzer |
| CN207636603U (en) * | 2017-12-12 | 2018-07-20 | 深圳拓真生物科技有限公司 | It is incubated cleaning device and immunity analysis instrument |
-
2018
- 2018-07-24 CN CN201810820822.2A patent/CN108732163B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105445258A (en) * | 2016-01-04 | 2016-03-30 | 泰州泽成生物技术有限公司 | Integrated cleaning and detecting device for chemiluminescence instrument |
| CN207181272U (en) * | 2017-09-28 | 2018-04-03 | 深圳市锦瑞生物科技有限公司 | Magnetic bead cleans separator and immunity analysis instrument automatically |
| CN208588675U (en) * | 2018-07-24 | 2019-03-08 | 山东博科诊断科技有限公司 | Full-automatic chemiluminescence immunoassay analysis meter |
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