CN108719144A - A kind of family selective breeding method of red line strain Mandarin fish - Google Patents
A kind of family selective breeding method of red line strain Mandarin fish Download PDFInfo
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- CN108719144A CN108719144A CN201810390881.0A CN201810390881A CN108719144A CN 108719144 A CN108719144 A CN 108719144A CN 201810390881 A CN201810390881 A CN 201810390881A CN 108719144 A CN108719144 A CN 108719144A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
- A01K61/95—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination specially adapted for fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention relates to a kind of family selective breeding methods of red line strain Mandarin fish, the described method comprises the following steps：（1）Pick out that body colour is yellowish in the Mandarin fish group of variation in body color, body surface lines takes on a red color and has the red line Mandarin fish of apparent growth vigor as spare parent；（2）After preferred female male parent is carried out adaptability reinforced cultivating, carries out artificial orientation's mating by male and female a pair, hastens parturition, each pair of produced fertilized eggs of parent population move to hatching barrel and individually cultivated；（3）Juvenile fish stage, adult fish stage in Mandarin fish growth, it is preferred that going out basic family of the high family of red line Mandarin fish ratio height, rate of body weight gain as next-generation selection and breeding, pass through continuous 4-6 as stated above for selection and breeding, it can cultivate in bright gay color, build is symmetrically graceful, it is physically fit, the significantly red line strain Mandarin fish of varietal characteristic.The present invention is effectively improved the problem that red line Mandarin fish kind is miscellaneous and degeneration is serious；Inbred is avoided, population genetic variations diversity is kept；From parent to seed, repeatedly preferably, Breeding Efficiency is high.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of family selective breeding method of red line strain Mandarin fish.
Background technology
Mandarin fish（siniperca chuatsi）It is homophonic with " expensive remaining ", signify " wealth and rank is had a surplus ".Normal body colour Mandarin fish, head
It is in light/dark balance that, which there are 3 black lines, side in portion,.Red line Mandarin fish is that the individual come is differentiated from common Mandarin fish group, the 3 of head
The variation of lines is red, and side is in faint yellow.Currently, the breeding of Mandarin fish is mainly carried out using the speed of growth as objective trait
Seed selection, breeding, and using body colour as objective trait progress family selective breeding research there is not been reported.Red line Mandarin fish color is gorgeous
It is beautiful, more add ornamental value, economic value, very characteristic and application value on the basis of mandarin fish implied meaning " wealth and rank is had a surplus ".
But effective conservation, breeding technique measure are lacked to red line Mandarin fish at present so that red line Mandarin fish body colour is light, it is variegated it is more, lose
The problem of diversity reduces is passed to be on the rise.Therefore, red line Mandarin fish select with the breeding that family selective breeding is basic route
It educates extremely urgent.
Invention content
In order to improve the genetic diversity of red line Mandarin fish, realizes and significantly obtain the uniform red line Mandarin fish of color and luster, originally
Invention provides a kind of family selective breeding method of red line strain Mandarin fish.
The present invention effectively combines two kinds of Breeding Traits of red line and weight, carries out family selective breeding to red line Mandarin fish, passes through
Mostly for continuous selection and breeding, pure red line Mandarin fish proportion obviously increases in filial generation, the preferred red line Mandarin fish family speed of growth
Also it significantly improves.
A kind of family selective breeding operating procedure of red line strain Mandarin fish is as follows：
（1）It is preferred that parent
Pick out that body colour is yellowish, body surface lines takes on a red color in the Mandarin fish group of variation in body color, and weight is larger, physique is strong
Red each 30-50 tails of line Mandarin fish male and female parent population strong, coefficient of condition is high；
（2）Establish family
（2.1）Parent's reinforced cultivating
Each 30-50 tails parent population of male and female is put into net cage, male and female separately carry out adaptability reinforced cultivating；It is super that net cage is placed on the depth of water
It crosses in 2 meters of water body, the red line Mandarin fish parent population of 5-8 tails is put into each net cage, agreeable to the taste living bait is fed, continuously cultivates 3-5
Week makes red line Mandarin fish enhancing physique and adapt to cage rearing environment；
（2.2）Combo between family
30-50 number net cage is set altogether；When water temperature reaches 22-28 DEG C, combo between red line Mandarin fish family is carried out, each
It is put into a pair of red line Mandarin fish by male and female in number net cage, and carries out PIT labels；
（2.3）Artificial induced spawning and insemination
It hastens parturition to the red line Mandarin fish oxytocin injection in each number net cage, the flow for being 0.1-0.2m/s with flow velocity is pierced
Swash, promotes parent population heat；18-24h after oxytocin injection checks ovary and testis development situation, when gently pressure Mandarin fish abdomen has
When ovum or sperm flow out, the good red line Mandarin fish of a pair of combo is taken out from number net cage, artificial insemination is carried out, is fertilized
Ovum；
（2.4）Hatching
Fertilized eggs are put into Lotic hatching bucket and are hatched, marks on Lotic hatching bucket and is identically numbered with number net cage；
The fry hatched is raised from the number net cage that Lotic hatching bucket is put into identical number, feeds agreeable to the taste fresh food fish, one
Fry in number net cage is a family；
（3）The red line strain Mandarin fish of selection and breeding
（3.1）Juvenile fish stage
When fry grows up to juvenile fish, when average body is up to 7-10cm, select that body colour is yellowish, body surface lines takes on a red color from each family
Juvenile fish 100-200 tails preferably go out Body Mass Index 50-60 tail juvenile fish in the top after measuring weight from 100-200 tail juvenile fish,
Then PIT labels are carried out one by one, and each family marks 50-60 tails；The juvenile fish of 30-50 family after label is put into same
In 3-5 mus of ponds, mixed with pond and cultivate；
（3.2）Adult fish stage
By mixing raising in 5-6 months, the weight of red line Mandarin fish was measured again, is averaged to the red line Mandarin fish of each family
Weight is ranked up, and is picked out the faint yellow 5-10 red line Mandarin fish familys apparent, that lines is redder of in the top and body surface and is made
For 1st generation parent；
Again according to step（1）-（3）2nd generation selection and breeding are carried out, 2nd generation parent is obtained；
By 4-6 for continuous selection and breeding, obtain that body weight increase is very fast and body surface is faint yellow and the red significantly red line strain Mandarin fish of line
Parent；
Selected red line Mandarin fish parent head has 3 to originate in the corners of the mouth, terminates on rear side of the gill cover, and be continuous through the red of head
Line, 2 pass through from eye side, and 1 passes through from the crown；Side is in mainly faint yellow in addition to red line.
The technical solution further limited is as follows：
The step（2）In, the net cage：1.5-3 meters long, 1.5-3 meters of width, 1.5-2 meters high, the mesh 30-60 mesh of net cage, net
Case is placed in the water body of pond, outdoor tank-culture or indoor greenhouse；Red line Mandarin fish overall length is not to be exceeded in the specification of bait fish
30-50%, day feeding rate 5-8%.
The step（2）Family between in combo, be in 45° angle from Mandarin fish dorsal fin base portion, depth 0.8cm or so is squeezed into
PIT is marked, to trace to the source parent.
The step（2）Artificial induced spawning and insemination in, by luteinizing hormone-releasing hormone analog（LRH-A）And suede
Chorionic Gonadotropin（HCG）It is uniformly mixed obtained oxytocins；The injection dosage of raun oxytocins is 15-20 μ g LRH-A+
The injection dosage of 1000-1500IU HCG/Kg, milter oxytocins halve；When artificial insemination, ovum, sperm are squeezed out simultaneously, in
A moment is stirred in washbowl, becomes fertilized eggs.
The advantageous effects of the present invention embody in the following areas：
1. two kinds of Breeding Traits of red line and weight are effectively combined as criterion for breeding by the present invention, Breeding Traits are intuitive, can be fast
Speed, precise Identification go out to meet the red line Mandarin fish individual of breeding goal character.
2. by constant generations selection and breeding, the red line Mandarin fish family screened, growth speed improves 15% or more, and red
Line individual proportion is more than 80%.
3. the problem of using family selective breeding method, being effectively improved red line Mandarin fish inbred and variety deterioration keeps red
The genetic structure diversity of line Mandarin fish.
4. using artificial insemination, the quality and developing synchrotron of fertilized eggs ensure that, compared with natural spawning, fertilization
Rate is increased to 85% or more.
5. a pair preferred red line Mandarin fish is cultivated after carrying out PIT labels with pond mixing, varying environment is avoided to cause family
Influence, ensure that the consistency of selection and breeding condition.
6. in each key point of red line Mandarin fish growth and development（Parent population, juvenile fish, adult fish）Carry out weight, a body
Excellent, quickening Breeding Efficiency and process are selected in the stringent screening of color in excellent.
Specific implementation mode
With reference to embodiment, the invention will be further described.
Embodiment 1
A kind of family selective breeding of red line strain Mandarin fish is carried out by following operating procedure：
（1）It is preferred that parent
Pick out that body colour is yellowish from the Mandarin fish group of 800 urosome chromatic variations, the red line Mandarin fish that body surface lines takes on a red color is female
Male each 120 tail of parent population, after measuring weight, therefrom the red line Mandarin fish of male and female larger, physically fit, that coefficient of condition is high of preferred weight is each
40 tails alternately parent population.
（2）Establish family
（2.1）Parent's reinforced cultivating
The alternative parent population male and female isolation of 80 tails is put into 1.5 meters long, 1.5 meters wide, high 2 meters of net cage, 60 mesh of mesh of net cage, into
Row adaptability reinforced cultivating.Net cage is placed in the pond of 2.5 meters of the depth of water, and the red line Mandarin fish of 5 tails male is put into each net cage
Or 5 red line Mandarin fish of tail female, male and female respectively put 8 net cages, it is bait fish to feed dace, is fed daily 2 times, day feeding volume is fish
The 5% of weight, it is continuous to cultivate 5 weeks.
（2.2）Combo between family
40 number net cages are set altogether；When water temperature reaches 24 DEG C, red line Mandarin fish family combo is carried out, in each number net cage
In be respectively put into a pair of red line Mandarin fish of male and female.It is in 45° angle from Mandarin fish dorsal fin base portion, depth 0.8cm or so squeezes into PIT marks
Note, to trace to the source parent.
（2.3）Artificial induced spawning and insemination
Oxytocins is by luteinizing hormone-releasing hormone analog（LRH-A）And human chorionic gonadtropin（HCG）It is uniformly mixed
It is made, injection dosage raun is 15 μ g LRH-A+1000IU HCG/Kg, and the injection dosage of milter oxytocins halves.To each volume
Red line Mandarin fish oxytocin injection in number net cage is hastened parturition, and the flow for being 0.1-0.2m/s with flow velocity stimulates, and promotes parent population
Heat；18-24h after oxytocin injection checks ovary and testis development situation, when gently pressure Mandarin fish abdomen has ovum or sperm outflow
When, the good red line Mandarin fish of a pair of combo is taken out from number net cage, is carried out artificial insemination, is obtained fertilized eggs.
（2.4）Hatching
Fertilized eggs are transferred in the Lotic hatching bucket of reference numeral and are hatched, the fry hatched is placed again into corresponding number
Number net cage in raise, feed grass carp, black carp, bream, silver carp etc. be used as mandarin fish bait, 2 times a day, day feeding volume is fish body
The 5% of weight.Fry in one number net cage is a family.
（3）The red line strain Mandarin fish of selection and breeding
（3.1）Juvenile fish stage
When fry grows up to juvenile fish, when average body is up to 8cm, pick out that body colour is yellowish, and body surface lines is in from each number net cage
Red red 120 tail of line Mandarin fish sorts after measuring weight, selects the red line Mandarin fish of weight 50 tails in the top and carry out one by one
PIT is marked, each 50 tail of family label；The juvenile fish of 40 familys after label is put into mix in 6 mu of ponds and is cultivated.
（3.2）Adult fish stage
After raising 6 months, the weight of red line Mandarin fish is measured again, the average weight of the red line Mandarin fish of each family is arranged
Sequence picks out faint yellow apparent, the 8 red line Mandarin fish familys that lines is redder of in the top and body surface as next generation's selection and breeding
Parent.
Each 40 tail of the red line Mandarin fish parent of male and female is picked out in 8 familys that previous generation preferably goes out and carries out combo, to keep away
Exempt from inbreeding, the female male parent of family selected by next-generation selection and breeding cannot come from 1 family, again according to the above method
40 red line Mandarin fish familys are established, by the continuous selection and breeding of 5 generations, it is bright-coloured and have apparent growth vigor preferably to go out 4 red lines,
Character stablizes the red line Mandarin fish family of heredity.This 4 family red line Mandarin fish heads have 3 to originate in the corners of the mouth, terminate at the gill
Lid rear side, and it is continuous through the red line on head, 2 pass through from eye side, and 1 passes through from the crown；Side is in mainly light in addition to red line
Yellow；Red line individual proportion is more than 80%；The speed of growth compared with original parent improve 18%, Breeding Effect is notable, continue by
This method selection and breeding can be obtained the red line strain Mandarin fish close to purifying.
Embodiment 2
A kind of family selective breeding of red line strain Mandarin fish is carried out by following operating procedure：
（1）It is preferred that parent
Pick out that body colour is yellowish from the Mandarin fish group of 1000 urosome chromatic variations, the red line Mandarin fish that body surface lines takes on a red color is female
Male each 150 tail of parent population, after measuring weight, therefrom the red line Mandarin fish of male and female larger, physically fit, that coefficient of condition is high of preferred weight is each
50 tails alternately parent population.
（2）Establish family
（2.1）Parent's reinforced cultivating
The alternative parent population male and female isolation of 100 tails is put into 2 meters long, 2 meters wide, high 1.5 meters of net cage, 60 mesh of mesh of net cage, into
Row adaptability reinforced cultivating, net cage are placed in the greenhouse cement pit of 2 meters of the depth of water, and the red line of 5 tails male is put into each net cage and is stuck up
Mouth mandarin fish or the red line Mandarin fish of 5 tails female, male and female respectively put 10 net cages, and it is bait fish to feed dace, is fed daily 2 times, day feeds
Amount is the 8% of fish body weight, continuous to cultivate 4 weeks.
（2.2）Combo between family
50 number net cages are set altogether；When water temperature reaches 23 DEG C, red line Mandarin fish family combo is carried out, in each number net cage
In be respectively put into the red line Mandarin fish of a pair of of male and female.It is in 45° angle from Mandarin fish dorsal fin base portion, depth 0.8cm or so squeezes into PIT marks
Note, to trace to the source parent.
（2.3）Artificial induced spawning and insemination
Oxytocins is by luteinizing hormone-releasing hormone analog（LRH-A）And human chorionic gonadtropin（HCG）It is uniformly mixed
It is made, injection dosage raun is 18 μ g LRH-A+1500IU HCG/Kg, and the injection dosage of milter oxytocins halves.To each volume
Red line Mandarin fish oxytocin injection in number net cage is hastened parturition, and the flow for being 0.1-0.2m/s with flow velocity stimulates, and promotes parent population
Heat；18-24h after oxytocin injection checks ovary and testis development situation, when gently pressure Mandarin fish abdomen has ovum or sperm outflow
When, the good red line Mandarin fish of a pair of combo is taken out from number net cage, is carried out artificial insemination, is obtained fertilized eggs.
（2.4）Hatching
Fertilized eggs are transferred in the Lotic hatching bucket of reference numeral and are hatched, the fry hatched is placed again into corresponding number
Number net cage in raise, feed grass carp, black carp, bream, silver carp etc. be used as mandarin fish bait, 2 times a day, day feeding volume is fish body
The 8% of weight.Fry in one number net cage is a family.
（3）The red line strain Mandarin fish of selection and breeding
（3.1）Juvenile fish stage
When fry grows up to juvenile fish, when average body is up to 10cm, pick out that body colour is yellowish, and body surface lines is in from each number net cage
Red red 150 tail of line Mandarin fish sorts after measuring weight, selects the red line Mandarin fish of weight 60 tails in the top and carry out one by one
PIT is marked, each 60 tail of family label；The juvenile fish of 50 familys after label is put into mix in 8 mu of ponds and is cultivated.
（3.2）Adult fish stage
After raising 5 months, the weight of red line Mandarin fish is measured again, the average weight of the red line Mandarin fish of each family is arranged
Sequence picks out the faint yellow 10 red line Mandarin fish familys apparent, that lines is redder of in the top and body surface and is used as next-generation selection and breeding
Parent.
Each 50 tail of the red line Mandarin fish parent of male and female is picked out in 10 familys that previous generation preferably goes out and carries out combo, to keep away
Exempt from inbreeding, the female male parent of family selected by next-generation selection and breeding cannot come from 1 family, again according to the above method
50 red line Mandarin fish familys are established, by the continuous selection and breeding of 4 generations, it is bright-coloured and have apparent growth vigor preferably to go out 5 red lines,
Character stablizes the red line Mandarin fish family of heredity.This 5 family red line Mandarin fish heads have 3 to originate in the corners of the mouth, terminate at the gill
Lid rear side, and it is continuous through the red line on head, 2 pass through from eye side, and 1 passes through from the crown；Side is in mainly light in addition to red line
Yellow；Red line individual proportion is more than 85%；The speed of growth compared with original parent improve 20%, Breeding Effect is notable, continue by
This method selection and breeding can be obtained the red line strain Mandarin fish close to purifying.
Embodiment 3
A kind of family selective breeding of red line strain Mandarin fish is carried out by following operating procedure：
（1）It is preferred that parent
Pick out that body colour is yellowish from the Mandarin fish group of 600 urosome chromatic variations, the red line Mandarin fish that body surface lines takes on a red color is female
Male each 100 tail of parent population, after measuring weight, therefrom the red line Mandarin fish of male and female larger, physically fit, that coefficient of condition is high of preferred weight is each
30 tails alternately parent population.
（2）Establish family
（2.1）Parent's reinforced cultivating
The alternative parent population male and female isolation of 60 tails is put into 2 meters long, 2 meters wide, high 1.5 meters of net cage, 60 mesh of mesh of net cage carries out
Adaptability reinforced cultivating, net cage are placed in the outdoor tank-culture of 2 meters of the depth of water, and the red line of 6 tails male is put into each net cage and sticks up mouth
Mandarin fish or the red line Mandarin fish of 6 tails female, male and female respectively put 5 net cages, and it is bait fish to feed dace, is fed daily 2 times, day feeding volume is
The 8% of fish body weight, it is continuous to cultivate 4 weeks.
（2.2）Combo between family
30 number net cages are set altogether；When water temperature reaches 23 DEG C, red line Mandarin fish family combo is carried out, in each number net cage
In be respectively put into the red line Mandarin fish of a pair of of male and female.It is in 45° angle from Mandarin fish dorsal fin base portion, depth 0.8cm or so squeezes into PIT marks
Note, to trace to the source parent.
（2.3）Artificial induced spawning and insemination
Oxytocins is by luteinizing hormone-releasing hormone analog（LRH-A）And human chorionic gonadtropin（HCG）It is uniformly mixed
It is made, injection dosage raun is 18 μ g LRH-A+1500IU HCG/Kg, and the injection dosage of milter oxytocins halves.To each volume
Red line Mandarin fish oxytocin injection in number net cage is hastened parturition, and the flow for being 0.1-0.2m/s with flow velocity stimulates, and promotes parent population
Heat；18-24h after oxytocin injection checks ovary and testis development situation, when gently pressure Mandarin fish abdomen has ovum or sperm outflow
When, the good red line Mandarin fish of a pair of combo is taken out from number net cage, is carried out artificial insemination, is obtained fertilized eggs.
（2.4）Hatching
Fertilized eggs are transferred in the Lotic hatching bucket of reference numeral and are hatched, the fry hatched is placed again into corresponding number
Number net cage in raise, feed grass carp, black carp, bream, silver carp etc. be used as mandarin fish bait, 2 times a day, day feeding volume is fish body
The 8% of weight.Fry in one number net cage is a family.
（3）The red line strain Mandarin fish of selection and breeding
（3.1）Juvenile fish stage
When fry grows up to juvenile fish, when average body is up to 8cm, pick out that body colour is yellowish, and body surface lines takes on a red color from each net cage
Red 100 tail of line Mandarin fish, measure weight after sort, select the red line Mandarin fish of weight 50 tails in the top and carry out PIT one by one
Label, each 50 tail of family label,；The juvenile fish of 30 familys after label is put into mix in 4 mu of ponds and is cultivated.
（3.2）Adult fish stage
After raising 5 months, the weight of red line Mandarin fish is measured again, the average weight of the red line Mandarin fish of each family is arranged
Sequence picks out faint yellow apparent, the 8 red line Mandarin fish familys that lines is redder of in the top and body surface as next generation's selection and breeding
Parent.
Each 30 tail of the red line Mandarin fish parent of male and female is picked out in 8 familys that previous generation preferably goes out and carries out combo, to keep away
Exempt from inbreeding, the female male parent of family selected by next-generation selection and breeding cannot come from 1 family, again according to the above method
30 red line Mandarin fish familys are established, by the continuous selection and breeding of 5 generations, it is bright-coloured and have apparent growth vigor preferably to go out 4 red lines,
Character stablizes the red line Mandarin fish family of heredity.This 4 family red line Mandarin fish heads have 3 to originate in the corners of the mouth, terminate at the gill
Lid rear side, and it is continuous through the red line on head, 2 pass through from eye side, and 1 passes through from the crown；Side is in mainly light in addition to red line
Yellow；Red line individual proportion more than 80%,；The speed of growth compared with original parent improve 15%, Breeding Effect is notable, continue by
This method selection and breeding can be obtained the red line strain Mandarin fish close to purifying.
Claims (4)
1. a kind of family selective breeding method of red line strain Mandarin fish, it is characterised in that concrete operation step is as follows：
（1）It is preferred that parent
Pick out that body colour is yellowish, body surface lines takes on a red color in the Mandarin fish group of variation in body color, and weight is larger, physique is strong
Red each 30-50 tails of line Mandarin fish male and female parent population strong, coefficient of condition is high；
（2）Establish family
（2.1）Parent's reinforced cultivating
Each 30-50 tails parent population of male and female is put into net cage, male and female separately carry out adaptability reinforced cultivating；It is super that net cage is placed on the depth of water
It crosses in 2 meters of water body, the red line Mandarin fish parent population of 5-8 tails is put into each net cage, agreeable to the taste living bait is fed, continuously cultivates 3-5
Week makes red line Mandarin fish enhancing physique and adapt to cage rearing environment；
（2.2）Combo between family
30-50 number net cage is set altogether；When water temperature reaches 22-28 DEG C, combo between red line Mandarin fish family is carried out, each
It is put into a pair of red line Mandarin fish by male and female in number net cage, and carries out PIT labels；
（2.3）Artificial induced spawning and insemination
It hastens parturition to the red line Mandarin fish oxytocin injection in each number net cage, the flow for being 0.1-0.2m/s with flow velocity is pierced
Swash, promotes parent population heat；18-24h after oxytocin injection checks ovary and testis development situation, when gently pressure Mandarin fish abdomen has
When ovum or sperm flow out, the good red line Mandarin fish of a pair of combo is taken out from number net cage, artificial insemination is carried out, is fertilized
Ovum；
（2.4）Hatching
Fertilized eggs are put into Lotic hatching bucket and are hatched, marks on Lotic hatching bucket and is identically numbered with number net cage；
The fry hatched is raised from the number net cage that Lotic hatching bucket is put into identical number, feeds agreeable to the taste fresh food fish, one
Fry in number net cage is a family；
（3）The red line strain Mandarin fish of selection and breeding
（3.1）Juvenile fish stage
When fry grows up to juvenile fish, when average body is up to 7-10cm, select that body colour is yellowish, body surface lines takes on a red color from each family
Juvenile fish 100-200 tails preferably go out Body Mass Index 50-60 tail juvenile fish in the top after measuring weight from 100-200 tail juvenile fish,
Then PIT labels are carried out one by one, and each family marks 50-60 tails；The juvenile fish of 30-50 family after label is put into same
In 3-5 mus of ponds, mixed with pond and cultivate；
（3.2）Adult fish stage
By mixing raising in 5-6 months, the weight of red line Mandarin fish was measured again, is averaged to the red line Mandarin fish of each family
Weight is ranked up, and is picked out the faint yellow 5-10 red line Mandarin fish familys apparent, that lines is redder of in the top and body surface and is made
For 1st generation parent；
Again according to step（1）-（3）2nd generation selection and breeding are carried out, 2nd generation parent is obtained；
By 4-6 for continuous selection and breeding, obtain that body weight increase is very fast and body surface is faint yellow and the red significantly red line strain Mandarin fish of line
Parent；
Selected red line Mandarin fish parent head has 3 to originate in the corners of the mouth, terminates on rear side of the gill cover, and be continuous through the red of head
Line, 2 pass through from eye side, and 1 passes through from the crown；Side is in mainly faint yellow in addition to red line.
2. a kind of family selective breeding method of red line strain Mandarin fish as described in claim 1, it is characterized in that：The step（2）
In, the net cage：1.5-3 meters long, 1.5-3 meters of width, 1.5-2 meters high, the mesh 30-60 mesh of net cage, net cage is placed on pond, room
In the water body of outer cement pit or indoor greenhouse；The 30-50% of red line Mandarin fish overall length, day bait throwing in is not to be exceeded in the specification of bait fish
Rate 5-8%.
3. a kind of family selective breeding method of red line strain Mandarin fish as described in claim 1, it is characterized in that：The step（2）
Family between in combo, be in 45° angle from Mandarin fish dorsal fin base portion, depth 0.8cm or so squeezes into PIT labels, so as to parent into
Row is traced to the source.
4. a kind of family selective breeding method of red line strain Mandarin fish as described in claim 1, it is characterized in that：The step（2）
Artificial induced spawning and insemination in, by luteinizing hormone-releasing hormone analog（LRH-A）And human chorionic gonadtropin（HCG）
It is uniformly mixed obtained oxytocins；The injection dosage of raun oxytocins is 15-20 μ g LRH-A+1000-1500IU HCG/Kg, male
The injection dosage of fish oxytocins halves；When artificial insemination, ovum, sperm are squeezed out simultaneously, a moment is stirred in washbowl, is become
Fertilized eggs.
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| CN110663591A (en) * | 2019-09-05 | 2020-01-10 | 池州市秋浦特种水产开发有限公司 | Breeding method of leopard flower mandarin fish |
| CN112154946A (en) * | 2020-11-05 | 2021-01-01 | 华中农业大学 | Method for cultivating indoor controllable inactivated bait fish with initial fish fries |
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| CN104430084A (en) * | 2014-11-25 | 2015-03-25 | 中国水产科学研究院淡水渔业研究中心 | Family breeding method for pearl white strain red tilapia |
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| WO2004089835A1 (en) * | 2003-04-01 | 2004-10-21 | Ebara Corporation | Method of treating sludge resulting from biological treatment with use of fish and apparatus therefor |
| CN103109758A (en) * | 2012-12-14 | 2013-05-22 | 化州市光辉养殖场有限公司 | Tilapia fry rearing method |
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| CN110663591A (en) * | 2019-09-05 | 2020-01-10 | 池州市秋浦特种水产开发有限公司 | Breeding method of leopard flower mandarin fish |
| CN112154946A (en) * | 2020-11-05 | 2021-01-01 | 华中农业大学 | Method for cultivating indoor controllable inactivated bait fish with initial fish fries |
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