CN101032228A - Establishing and culture selecting method of breeding a pure system of fugu obscurus - Google Patents
Establishing and culture selecting method of breeding a pure system of fugu obscurus Download PDFInfo
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- CN101032228A CN101032228A CNA2006100245216A CN200610024521A CN101032228A CN 101032228 A CN101032228 A CN 101032228A CN A2006100245216 A CNA2006100245216 A CN A2006100245216A CN 200610024521 A CN200610024521 A CN 200610024521A CN 101032228 A CN101032228 A CN 101032228A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The process of selectively breeding and establishing pure cultivated Fugu obscurus line includes the following steps: 1. selecting parent fishes with high vitality, great weight and normal body characteristic, selecting candidate family line, separate raising of each fish, and selecting excellent selfing line as the basic line for the next step of selective breeding; 2. optimizing the pure line in the key growth stages of young fish stage, commercial fish stage and mature stage; and 3. repeating the foregoing steps for 3-5 periods to establish the pure cultivated Fugu obscurus line through the inbreeding of female and male fishes inside Fugu obscurus line. The present invention is superior in that by means of the inbreeding of female and male fishes inside Fugu obscurus line, it is possible to research the genetic characteristic and variety differentiating status of Fugu obscurus.
Description
Technical field the present invention relates to culture the seed selection of fugu obscurus improved seeds and the directive breeding technology of nontoxic breed pure system of fugu obscurus, is the foundation and the selection of culturing pure system of fugu obscurus specifically.
Background technology is present, and the work of China's fish breeding is in the starting stage, by crossbreeding, colony's seed selection, the breeding of chromosome set engineering, comprehensive breeding, technical methods such as genetic engineering breeding are material with carp, crucian carp, triangular bream, Tilapia mossambica, select ten several fish new lines.
Family selective breeding is as the important means of animal varieties improvement, in the seed selection of fowl poultry kind, brought into play important function, huge economic benefit and social benefit have been produced, the breed improvement of aquatic livestock has bigger difficulty owing to its breed characteristics and reproductive biology characteristic, though the breed improvement of fish has in recent years obtained very big achievement, but the patent that relates to fugu obscurus both at home and abroad has only artificial propagation CN98111600.0 and control poison to culture two patents of CN02138614.5, the patent report that does not also relate to perfect fugu obscurus family and pure line selection, the present invention according to the reproductive biology characteristics design of fugu obscurus one cover complete, and be practical, outstanding effect is cultured the technical method of pure system of fugu obscurus seed selection, cultures the nontoxic pure lines of fugu obscurus for directive breeding and lays a good foundation.
Summary of the invention for achieving the above object, the technical scheme that the present invention takes is: 1) in parent population, optimize energetic, physical trait is normal, body weight is greater than the parent population of average weight 15～20%, as alternative parent population, selection intensity is 1～2%, the raun of each post-coitum is cultured respectively in a spawning pond, beginning is selected the high individuality of the big incubation rate of egg laying amount again as candidate family from laying eggs, from laying eggs to growing seedlings, form, the offspring of each bar fugu obscurus raises respectively, set up family, constantly eliminate during the breed and grow in the family slowly, the individuality of resistance difference and growth are slow, the family of resistance difference, the family that optimizes is as the basic family of seed selection of future generation; 2) the critical period young stage of fugu obscurus growth, marketable fish phase, the prematuration period three phases be the family selective breeding critical period, carry out preferred; 3) repeating step 1)-2) three to five cycles, by the inbreeding of female milter in the fugu obscurus family, set up and culture pure system of fugu obscurus.
The mating of described female milter is meant the principle by a milter and three raun mating, make selected milter and selected raun mating by artificial insemination, article three, raun institute postpartum is on behalf of with the different female family half sibs of father, every raun institute postpartum on behalf of with the father with female family full-sibs, 1-10 family is as the basic family as pure line selection.
After described young stage is meant that preferably every fish lays eggs, statistics egg laying amount and incubation rate, be the parent population of selecting incubation rate will set up family in the individuality of ten thousand ovum of 20-30 at first in the conduct of the individuality more than 90% at egg laying amount, the selection vigor is good behind the fry hatching, the strong fry of phototaxis is as initial seed selection, the fry that selected each bar parent population is given birth to is cultured separately in a nursery pond, supported to the juvenile fish that can sell, the juvenile fish of each family is cultured separately in one or several and cultures in the pond.
Described marketable fish preferably was meant in the marketable fish breed phase, add up the survival rate of each family, the average weight of marketable fish, body is long, body colour, biology and commodity indexs such as the uniformity are eliminated the family of about 2/3 at the bottom of growth rate and the survival rate, and about 1/3 the family that only select and remain fast growth and survival rate are high gives over to standby parent population.
Described to the prematuration period preferably be meant from each family of being supported, select individual big, the standby parent population that energetic individual 50-100 sets up family to conduct, accelerating, grow seedlings, proceed seed selection, each family finally only keeps the offspring of 3-5 female fish as the seed of going down to posterity, and the seed of every fish of the family that goes down to posterity is cultured separately from start to finish.During the parent population mating, setting up self-fertilization family (sff), is to carry out mating between offspring's the male and female individuality of same fish.
Repeating step 1)-2 the step 3 to five of basic family selective breeding time), by in the fugu obscurus family each generation female milter inbreeding, set up 1-30 nontoxic breed pure system of fugu obscurus.
The present invention has following advantage: by the foundation to the different pure lines of breed fugu obscurus, directive breeding is nontoxic, the degeneration-resistant new lines of suitable China breeding environment of high-quality, high yield, stable yields, and the inbreeding by female milter in mating between the different pure lines of fugu obscurus and the family, the genetics characteristic of research fugu obscurus and the kind differentiation situation nontoxic fugu obscurus new lines of purificating and rejuvenating.
Embodiment
1. in 5000 pairs of parent populations, optimize energetic, physical trait is normal, body weight greater than 500 pairs of the parent populations of average weight 15～20% as alternative parent population, the female fish fertilized egg of each post-coitum is put in respectively in the hatching cylinder hatches, select the high individuality of the big incubation rate of egg laying amount as candidate family, from laying eggs to growing seedlings, form, the offspring of each bar fugu obscurus raises respectively, optimize 150 familys, constantly eliminate during the breed and grow in the family slowly, the individuality of resistance difference and growth are slow, the family of resistance difference, only select growth fast in the residue family, the survival rate height, comparatively uniform 50 familys of size are as the basic family of seed selection of future generation;
2. utilize the fugu obscurus artificial propagation techniques, principle by a milter and three raun mating, make selected milter and selected raun mating by artificial insemination, three raun institute postpartum is on behalf of with the different female family half sibs of father, every raun institute postpartum on behalf of with the father with female family full-sibs.
The critical period young stage of fugu obscurus growth, marketable fish phase, the prematuration period three phases be the family selective breeding critical period, carry out preferred.
1. young stage: after every fish lays eggs, statistics egg laying amount and incubation rate, a general raun egg laying amount is ten thousand ovum of 20-30, at egg laying amount is the parent population of selecting incubation rate will set up family in the conduct of the individuality more than 90% in the individuality of ten thousand ovum of 20-25, the selection vigor is good behind the fry hatching, the strong fry of phototaxis is as initial seed selection family, the fry that selected each bar parent population is given birth to is cultured separately in a nursery pond, support to the juvenile fish that can sell, the juvenile fish of each family is cultured separately in one or several and cultures in the pond.
2. marketable fish phase: survival rate is generally 50-80% in the marketable fish breed phase, the marketable fish of each family all has the 5000-10000 bar, add up the survival rate of each family, the average weight of marketable fish, body is long, body colour, biology and commodity indexs such as the uniformity, to be higher than the standard of average weight 15-20%, to optimize 500-1000 marketable fish is cultured proximity maturation (ovary 2-3 phase).
3. the prematuration period: it is bigger to select body weight from 150 familys of being supported, body is longer, the body colour and the uniformity be 50 familys preferably, as the family that finally goes down to posterity, each family is preferably energetic, the standby parent population that individual big individual 50-100 sets up family to conduct, accelerating, grow seedlings, proceed seed selection, other parent population can be used as production or sells.
4. the different female family half sibs of the same father that will select and with the father with the basic family of female family full-sibs as seed selection of future generation, the step 2 of this family of repeating group seed selection)-3) three to five cycles, by each sets up 1-10 nontoxic breed pure system of fugu obscurus for the inbreeding of female milter in the fugu obscurus family.
During the parent population mating, for setting up self-fertilization family (sff), can make between offspring's the male and female individuality of same fish and carry out mating, directive breeding is nontoxic, the degeneration-resistant new lines of suitable China breeding environment of high-quality, high yield, stable yields, and the inbreeding by female milter in mating between the different pure lines of fugu obscurus and the family, the genetics characteristic of research fugu obscurus and the kind differentiation situation nontoxic fugu obscurus new lines of purificating and rejuvenating.
The standard of pure line selection comprises: the body weight of parent population, long, the color of body, the egg laying amount of raun, incubation rate, juvenile fish vigor, the disease-resistant degeneration-resistant ability of the uniformity of marketable fish, average weight, body length, each family.This technology is applicable to that fairly large nursery or cultivation base carry out the breed improvement of fugu obscurus, and little nursery can be united mutually, according to strength and the scale of oneself, determines to set up separately what of pure lines, carries out the exchange of raun or milter then.What of parent population quantity the population scale that wherein is used for seed selection promptly use, and can do suitable adjustment according to the scale of cultivation base and nursery.
Claims (7)
1. culture the foundation and the selection of pure system of fugu obscurus, it is characterized in that: 1) in same family parent population, optimize energetic, physical trait is normal, body weight is greater than the parent population of average weight 15～20%, as alternative parent population, selection intensity is 10～20%, the raun of each post-coitum is cultured respectively in a spawning pond, beginning is selected the high individuality of the big incubation rate of egg laying amount again as candidate family from laying eggs, from laying eggs to growing seedlings, form, the offspring of each bar fugu obscurus raises respectively, set up self-fertilization family (sff), constantly eliminate during the breed and grow in the self-fertilization family (sff) slowly, the individuality of resistance difference and growth are slow, the self-fertilization family (sff) of resistance difference, the self-fertilization family (sff) that optimizes is as the basic family of seed selection of future generation; 2) the critical period young stage of fugu obscurus growth, marketable fish phase, the prematuration period three phases be the self-fertilization family (sff) seed selection critical period, carry out preferred; 3) repeating step 1)-2) three to five cycles, by the inbreeding of female milter in the fugu obscurus self-fertilization family (sff), set up and culture pure system of fugu obscurus.
2. according to the foundation and the selection of the described breed pure system of fugu obscurus of claim 1, it is characterized in that: by the principle of a milter and three raun mating, make selected milter and selected raun mating by artificial insemination, article three, raun institute postpartum is on behalf of with the different female family half sibs of father, every raun institute postpartum on behalf of with the father with female family full-sibs, 1-10 family is as the basic family as pure line selection.
3. according to the foundation and the selection of the described breed pure system of fugu obscurus of claim 1, it is characterized in that: after described young stage is meant that preferably every fish lays eggs, statistics egg laying amount and incubation rate, be the parent population of selecting incubation rate will set up family in the individuality of ten thousand ovum of 20-30 at first in the conduct of the individuality more than 90% at egg laying amount, the selection vigor is good behind the fry hatching, the strong fry of phototaxis is as initial seed selection, the fry that selected each bar parent population is given birth to is cultured separately in a nursery pond, support to the juvenile fish that can sell, the juvenile fish of each family is cultured separately in one or several and cultures in the pond.
4. according to the foundation and the selection of the described breed pure system of fugu obscurus of claim 1, it is characterized in that: described marketable fish preferably was meant in the marketable fish breed phase, add up the survival rate of each family, the average weight of marketable fish, body is long, body colour, biology and commodity indexs such as the uniformity, eliminate the family of about 2/3 at the bottom of growth rate and the survival rate, about 1/3 the family that only select and remain fast growth and survival rate are high gives over to standby parent population.
5. according to the foundation and the selection of the described breed pure system of fugu obscurus of claim 1, it is characterized in that: preferably be meant the prematuration period of described and from each family of being supported, select individuality greatly, the standby parent population that energetic individual 50-100 sets up family to conduct, accelerating, grow seedlings, proceed seed selection, each family finally only keeps the offspring of 3-5 female fish as the seed of going down to posterity, and the seed of every fish of the family that goes down to posterity is cultured separately from start to finish.
6. according to the foundation and the selection of the described breed pure system of fugu obscurus of claim 1, it is characterized in that: the self-fertilization family (sff) of setting up during the parent population mating is to carry out mating between offspring's the male and female individuality of same fish.
7. according to the foundation and the selection of the described breed pure system of fugu obscurus of claim 1, it is characterized in that: repeating step 1)-2) step 3 to five cycle of basic family selective breeding, by each is set up 1-10 and culture pure system of fugu obscurus for the inbreeding of female milter in the fugu obscurus family.
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| CN2006100245216A CN101032228B (en) | 2006-03-09 | 2006-03-09 | Establishing and selecting method for breeding a pure system of fugu obscurus |
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| CN2006100245216A CN101032228B (en) | 2006-03-09 | 2006-03-09 | Establishing and selecting method for breeding a pure system of fugu obscurus |
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| CN101032228A true CN101032228A (en) | 2007-09-12 |
| CN101032228B CN101032228B (en) | 2010-07-21 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940185A (en) * | 2010-09-28 | 2011-01-12 | 上海能正渔业科技开发有限公司 | Staged population selective breeding method of Takifugu obscurus non-toxic pedigree |
| CN101305701B (en) * | 2008-07-08 | 2011-01-26 | 中国水产科学研究院黄海水产研究所 | Fish family establishing and disease-resistant high yield fine breeding method |
| CN102187839A (en) * | 2011-06-02 | 2011-09-21 | 中国水产科学研究院淡水渔业研究中心 | Artificial crossing cultivation method of seawater takifugu species |
| CN102273416A (en) * | 2011-06-02 | 2011-12-14 | 中国水产科学研究院淡水渔业研究中心 | Method for promoting synchronous development of sexual glands of takifugu obscurus male and female parent fishes |
| CN102349462A (en) * | 2011-08-30 | 2012-02-15 | 吴江市水产养殖有限公司 | Breeding method for improved variety of fishes |
| CN101461336B (en) * | 2008-12-10 | 2012-02-22 | 大湖水殖股份有限公司 | Method for establishing and breeding multiresistance fish population, variety (strain) and family |
| CN102405884A (en) * | 2011-09-30 | 2012-04-11 | 吴江市水产养殖有限公司 | Rapid breeding method of new fish strain |
| CN102599079A (en) * | 2011-01-24 | 2012-07-25 | 华中农业大学 | Rapid high-efficiency method for constructing half-sib family of Megalobrama Terminalis |
| CN104686423A (en) * | 2015-03-23 | 2015-06-10 | 江苏中洋集团股份有限公司 | Colony-family-molecule comprehensive genetic breeding method for takifugu obscurus |
| CN110004235A (en) * | 2019-05-13 | 2019-07-12 | 大连海洋大学 | A kind of relevant SNP site of fugu obscurus fast-growth and application |
| CN111670841A (en) * | 2020-07-08 | 2020-09-18 | 广西壮族自治区水产引育种中心 | Breeding method of new red tilapia strain |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1108747C (en) * | 1998-12-09 | 2003-05-21 | 中国科学院南京地理与湖泊研究所 | Artificial breeding technology for dark-figured oriental porpoise |
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- 2006-03-09 CN CN2006100245216A patent/CN101032228B/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305701B (en) * | 2008-07-08 | 2011-01-26 | 中国水产科学研究院黄海水产研究所 | Fish family establishing and disease-resistant high yield fine breeding method |
| CN101461336B (en) * | 2008-12-10 | 2012-02-22 | 大湖水殖股份有限公司 | Method for establishing and breeding multiresistance fish population, variety (strain) and family |
| CN101940185A (en) * | 2010-09-28 | 2011-01-12 | 上海能正渔业科技开发有限公司 | Staged population selective breeding method of Takifugu obscurus non-toxic pedigree |
| CN102599079B (en) * | 2011-01-24 | 2013-11-27 | 华中农业大学 | Method for constructing half-sib family of Megalobrama Terminalis |
| CN102599079A (en) * | 2011-01-24 | 2012-07-25 | 华中农业大学 | Rapid high-efficiency method for constructing half-sib family of Megalobrama Terminalis |
| CN102273416A (en) * | 2011-06-02 | 2011-12-14 | 中国水产科学研究院淡水渔业研究中心 | Method for promoting synchronous development of sexual glands of takifugu obscurus male and female parent fishes |
| CN102187839B (en) * | 2011-06-02 | 2012-08-08 | 中国水产科学研究院淡水渔业研究中心 | Artificial crossing cultivation method of seawater takifugu species |
| CN102187839A (en) * | 2011-06-02 | 2011-09-21 | 中国水产科学研究院淡水渔业研究中心 | Artificial crossing cultivation method of seawater takifugu species |
| CN102349462A (en) * | 2011-08-30 | 2012-02-15 | 吴江市水产养殖有限公司 | Breeding method for improved variety of fishes |
| CN102405884A (en) * | 2011-09-30 | 2012-04-11 | 吴江市水产养殖有限公司 | Rapid breeding method of new fish strain |
| CN104686423A (en) * | 2015-03-23 | 2015-06-10 | 江苏中洋集团股份有限公司 | Colony-family-molecule comprehensive genetic breeding method for takifugu obscurus |
| CN104686423B (en) * | 2015-03-23 | 2016-09-14 | 江苏中洋集团股份有限公司 | A kind of method of Puffer fish colony family molecular association genetic breeding |
| CN110004235A (en) * | 2019-05-13 | 2019-07-12 | 大连海洋大学 | A kind of relevant SNP site of fugu obscurus fast-growth and application |
| CN110004235B (en) * | 2019-05-13 | 2022-06-03 | 大连海洋大学 | SNP locus related to rapid growth of fugu obscurus and application |
| CN111670841A (en) * | 2020-07-08 | 2020-09-18 | 广西壮族自治区水产引育种中心 | Breeding method of new red tilapia strain |
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| CN101032228B (en) | 2010-07-21 |
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