CN108713641B - Chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive as well as preparation method and application thereof - Google Patents
Chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive, and a preparation method and application thereof. The compound feed additive comprises traditional Chinese medicine components and chitosan quaternary ammonium salt, wherein the traditional Chinese medicine components comprise the following raw materials in parts by weight: 10-30 parts of dried orange peel, 40-60 parts of astragalus membranaceus, 30-50 parts of radix isatidis, 20-40 parts of houttuynia cordata, 20-40 parts of pomegranate rind, 10-30 parts of radish seed, 15-35 parts of radix bupleuri, 20-40 parts of coptis chinensis and 8-20 parts of angelica sinensis; the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1:1 to 3. The components of the compound feed additive are matched with each other, so that the compound feed additive plays a role in synergy, and is safe and reliable; the composition has good prevention and/or treatment effects on the porcine inflammatory bowel disease, and can effectively reduce the occurrence rate of porcine diarrhea; can also improve the growth efficiency of pigs. In addition, the raw materials are low in price and easy to obtain, and have no residue, drug resistance and toxic or side effect.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive, and a preparation method and application thereof.
Background
The porcine inflammatory bowel disease is a common intestinal disease in pig production, generally mainly comprises infectious diseases caused by bacteria and viruses, including porcine epidemic diarrhea, dysentery, transmissible gastroenteritis and the like, and has the manifestation form of diarrhea, and is accompanied by symptoms of body temperature rise, inappetence, dehydration and the like. The slow growth of pigs, the reduced feed return, the reduced production performance and even the death of the pigs are caused, and the economic benefit of the breeding industry is seriously influenced.
Most of the traditional methods for treating inflammatory bowel diseases are to use some antibiotics for prevention and treatment, such as gentamicin, amoxicillin, norfloxacin and the like, but excessive use of these drugs may cause some adverse effects, such as drug resistance, toxic and side effects, drug residues and digestive tract flora imbalance, which cause the reduction of intestinal immune function of pigs and adverse effects on human bodies after eating these animals.
The Chinese herbal medicine is from nature, has wide resources and low price, has double functions of nutrition and medicine, has multiple effects and small toxic and side effects, takes the Chinese herbal medicine as the raw material, researches effective medicines or feed additives for preventing and/or treating inflammatory bowel diseases, and has great application value in the breeding industry.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive. The compound feed additive is obtained by compounding the chitosan quaternary ammonium salt and 9 traditional Chinese medicines serving as raw materials, and all the components are matched with each other to play a synergistic effect, so that the compound feed additive is safe and reliable; has good prevention and/or treatment effect on the porcine inflammatory bowel disease, and can also improve the growth efficiency of the pigs.
The invention also aims to provide a preparation method of the chitosan quaternary ammonium salt and the Chinese herbal medicine compound feed additive.
The invention further aims to provide application of the chitosan quaternary ammonium salt and the Chinese herbal medicine compound feed additive in preventing and/or treating the porcine inflammatory bowel disease.
The above object of the present invention is achieved by the following technical solutions:
the compound feed additive comprises traditional Chinese medicine components and chitosan quaternary ammonium salt, wherein the traditional Chinese medicine components comprise the following raw materials in parts by weight: 10-30 parts of dried orange peel, 40-60 parts of astragalus membranaceus, 30-50 parts of radix isatidis, 20-40 parts of houttuynia cordata, 20-40 parts of pomegranate rind, 10-30 parts of radish seed, 15-35 parts of radix bupleuri, 20-40 parts of coptis chinensis and 8-20 parts of angelica sinensis; the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 1-3.
Pericarpium Citri Tangerinae, Raphani semen, herba Houttuyniae, and bupleuri radix have effects of invigorating spleen, promoting appetite, astringing, and relieving diarrhea, and can be used for treating food stagnation, abdominal distention, bacillary dysentery, and diarrhea.
The coptis root, the isatis root and the pomegranate bark have the functions of clearing away heat and toxic materials, cooling blood and eliminating swelling, and are mainly used for treating acute and chronic hepatitis and resisting virus infection.
Huang Qi and Dang Gui have the actions of tonifying qi, consolidating superficial resistance, healing sores and promoting granulation, and can be used for qi deficiency, fatigue, chronic diarrhea and rectocele.
The chitosan is obtained by deacylation of chitin, belongs to an amino-containing homogeneous straight-chain polysaccharide derivative, and is a green feed additive with wide application prospect. Has broad-spectrum antibacterial activity, has obvious inhibition effect on gram-positive bacteria and candida albicans, and has the effects of immunity, protection and the like. The chitosan quaternary ammonium salt is a derivative of chitosan, and is obtained by converting an amino group of chitosan into a quaternary ammonium salt through introducing a group or attaching a low molecular quaternary ammonium salt to the amino group. The chitosan quaternary ammonium salt is a polycation compound, has the characteristics of good water solubility, flocculation property, biocompatibility, moisture absorption and retention property, antibacterial property, promotion of drug absorption and the like, and has a wide application range.
The chitosan quaternary ammonium salt is compounded with the traditional Chinese medicine components, and the chitosan quaternary ammonium salt can promote the absorption of the traditional Chinese medicine components, inhibit the growth of bacteria and enhance the immune function of organisms.
Preferably, the traditional Chinese medicine components comprise the following raw materials in parts by weight: 15-25 parts of dried orange peel, 45-55 parts of astragalus membranaceus, 35-45 parts of radix isatidis, 25-35 parts of houttuynia cordata, 25-35 parts of pomegranate rind, 15-25 parts of radish seed, 20-30 parts of radix bupleuri, 25-35 parts of coptis chinensis and 10-18 parts of angelica sinensis.
Preferably, the traditional Chinese medicine components comprise the following raw materials in parts by weight: 20 parts of dried orange peel, 50 parts of astragalus, 40 parts of isatis root, 30 parts of houttuynia, 30 parts of pomegranate bark, 20 parts of radish seed, 25 parts of radix bupleuri, 30 parts of coptis root and 15 parts of angelica.
Preferably, the chitosan quaternary ammonium salt is composed of chitosan quaternary ammonium salts with molecular weights of 800-1200, 2000-2500 and 40000-60000 in a mass ratio of 1-3: 1-3.
Preferably, the chitosan quaternary ammonium salt is composed of the chitosan quaternary ammonium salts with the molecular weights of 900-1100, 2100-2300 and 45000-55000 according to the mass ratio of 1-2: 1-2.
More preferably, the chitosan quaternary ammonium salt consists of chitosan quaternary ammonium salts with molecular weights of 1000, 2200 and 50000 according to a mass ratio of 1:1: 1.
Preferably, the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 2.
The invention also provides a preparation method of the compound feed additive. The preparation method comprises the following steps: selecting each traditional Chinese medicine in the formula, respectively removing impurities and non-medicinal parts of the medicines, ensuring that the water content of the medicinal parts is lower than 10%, mixing according to the formula amount, crushing, and sieving with a 100-200-mesh sieve; then evenly mixing the mixture with chitosan quaternary ammonium salt and drying to obtain the compound feed additive.
The application of the compound feed additive is also within the protection scope of the invention. The addition amount of the compound feed additive in the pig feed is 1-2% of the total mass.
Preferably, the addition amount of the compound feed additive in the pig feed is 1.5 percent of the total mass.
Compared with the prior art, the invention has the following beneficial effects:
the compound feed additive is obtained by compounding the chitosan quaternary ammonium salt and 9 traditional Chinese medicines serving as raw materials, and all the components are matched with each other to play a synergistic effect, so that the compound feed additive is safe and reliable; the composition has good prevention and/or treatment effects on the porcine inflammatory bowel disease, and compared with a control group, the diarrhea rate is remarkably improved; but also can improve the growth efficiency of the pigs. In addition, the raw materials adopted by the compound feed additive are low in price and easy to obtain, have double functions of nutrition and medicine, and have the advantages of no residue, drug resistance and no toxic or side effect.
Detailed Description
The present invention is further described in detail below with reference to specific examples, which are provided for illustration only and are not intended to limit the scope of the present invention. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1
A chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive comprise the following raw materials in parts by weight: 20 parts of dried orange peel, 50 parts of astragalus, 40 parts of isatis root, 30 parts of houttuynia, 30 parts of pomegranate rind, 20 parts of radish seed, 25 parts of radix bupleuri, 30 parts of coptis root and 15 parts of angelica; the molecular weight of the chitosan quaternary ammonium salt is 1000, 2200 and 50000 respectively, and the molecular weight of the chitosan quaternary ammonium salt is 100 g; the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 2.
the preparation method of the compound feed additive comprises the following steps:
(1) taking pericarpium citri reticulatae, astragalus membranaceus, radix isatidis, houttuynia cordata, pomegranate rind, radish seed, radix bupleuri, coptis chinensis and angelica sinensis, respectively removing impurities and non-medicinal parts of the medicaments, wherein the water content of the raw medicaments is lower than 10%, and weighing the selected raw medicaments according to the formula for later use;
(2) mixing the prepared raw materials, putting the raw materials into a grinder for grinding, and sieving the raw materials by a sieve of 100-120 meshes to obtain coarse powder for later use;
(3) mixing the coarse powder obtained in the last step with chitosan quaternary ammonium salt with molecular weight of 1000, 2200, 50000 respectively by a mixer, drying and packaging the mixed medicine to obtain the compound feed additive, and sealing and storing.
Example 2
A chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive comprise the following raw materials in parts by weight: 18 parts of dried orange peel, 48 parts of astragalus, 38 parts of isatis root, 28 parts of houttuynia, 28 parts of pomegranate bark, 18 parts of radish seed, 23 parts of radix bupleuri, 28 parts of coptis root and 13 parts of angelica; the molecular weight of the chitosan quaternary ammonium salt is 1000, 2200 and 50000 respectively, and the molecular weight of the chitosan quaternary ammonium salt is 100g, 200g and 300g respectively; the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 3.
the preparation method of the compound feed additive is the same as that of example 1.
Example 3
A chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive comprise the following raw materials in parts by weight: 22 parts of dried orange peel, 48 parts of astragalus, 42 parts of isatis root, 33 parts of houttuynia, 33 parts of pomegranate bark, 22 parts of radish seed, 28 parts of radix bupleuri, 33 parts of coptis root and 16 parts of angelica; the molecular weight of the chitosan quaternary ammonium salt is respectively 1000, 2200 and 50000, and the molecular weight of the chitosan quaternary ammonium salt is respectively 200g, 300g and 100 g; the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 1.
the preparation method of the compound feed additive is the same as that of example 1.
Example 4
A chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive comprise the following raw materials in parts by weight: 20 parts of dried orange peel, 48 parts of astragalus, 38 parts of isatis root, 28 parts of houttuynia cordata, 28 parts of pomegranate bark, 17 parts of radish seed, 25 parts of radix bupleuri, 28 parts of coptis chinensis and 15 parts of angelica; the molecular weight of the chitosan quaternary ammonium salt is 1000, 2200 and 50000 respectively, and the molecular weight of the chitosan quaternary ammonium salt is 300g, 100g and 200g respectively; the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 2.
the preparation method of the compound feed additive is the same as that of example 1.
Example 5
A chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive, the traditional Chinese medicine components are the same as example 1, and the difference lies in the molecular weight of the chitosan quaternary ammonium salt is 1000.
Example 6
A chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive, the traditional Chinese medicine components are the same as example 1, except that the molecular weight of the chitosan quaternary ammonium salt is 2200.
Example 7
A chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive, the traditional Chinese medicine components are the same as example 1, except that the molecular weight of the chitosan quaternary ammonium salt is 50000.
Example 8
A chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive, the traditional Chinese medicine components are the same as example 2, except that the molecular weight of the chitosan quaternary ammonium salt is 50000.
Example 9
A chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive are disclosed, wherein the traditional Chinese medicine components are the same as example 1, and the difference is that the chitosan quaternary ammonium salt is composed of components with the molecular weight of 1000 and 50000 according to the mass ratio of 1: 1.
Example 10
A chitosan quaternary ammonium salt and a Chinese herbal medicine compound feed additive are disclosed, wherein the traditional Chinese medicine components are the same as in example 1, and the difference is that the chitosan quaternary ammonium salt is composed of 2200 and 50000 according to the mass ratio of 1: 2.
Example 11 concrete application example
1. Application in weaned piglets
(1) The test method comprises the following steps: selecting 220 weaned piglets, and dividing into 22 groups, one group is blank control group, one group is control group, and the other groups are test groups. The blank control group and the control group are fed with common feed, and the feed fed by the test group is added with the compound feed additive in the embodiment. The breeding mode is free drinking water and free feeding, after 24h, the control group and the test group are inoculated with escherichia coli, the blank group is not inoculated, and the disease condition is recorded for 120 h.
(2) And (3) test results:
the test groups, the type of feed fed, and the test results are shown in table 1.
Wherein, only the traditional Chinese medicine components in the embodiment 1 are added in the comparative example 1, and the chitosan quaternary ammonium salt is not added; the comparative example 2 is that only the traditional Chinese medicine components in the example 2 are added, and the chitosan quaternary ammonium salt is not added; the comparative example 3 is that only the traditional Chinese medicine components in the example 3 are added, and the chitosan quaternary ammonium salt is not added; comparative example 4 is only adding the traditional Chinese medicine components in example 4, and no chitosan quaternary ammonium salt is added; comparative example 5 is only adding the chitosan quaternary ammonium salt in example 1, and no traditional Chinese medicine component; comparative example 6 only the chitosan quaternary ammonium salt of example 2 was added, and no herbal ingredients were added.
The mass ratio of the feed additive is the proportion of the feed additive in the total mass.
TABLE 1
| Group of | Feed stuff | Feed additive | Feed additive mass ratio (%) | Diarrhea Rate (%) |
| Blank control group | Common feed | - | - | Without change |
| Common control | Common feed | - | 0 | 100 |
| Comparative example 1 | Common feed | The herbal composition of example 1 | 1.5 | 70 |
| Comparative example 2 | Common feed | The herbal composition of example 2 | 1.5 | 70 |
| Comparative example 3 | Common feed | The Chinese medicinal composition of example 3 | 1.5 | 70 |
| Comparative example 4 | Common feed | Example 4 Chinese medicinal Components | 1.5 | 70 |
| Comparative example 5 | Common feed | Quaternary ammonium salt of chitosan of example 1 | 1.5 | 50 |
| Comparative example 6 | Common feed | Quaternary ammonium salt of chitosan of example 2 | 1.5 | 60 |
| Comparative example 7 | Common feed | Quaternary ammonium salt of chitosan of example 3 | 1.5 | 60 |
| Comparative example 8 | Common feed | Quaternary ammonium salt of chitosan of example 4 | 1.5 | 60 |
| Test 1 | Common feed | Example 1 Compound feed additive | 1 | 30 |
| Test 2 | Common feed | Example 1 Compound feed additive | 2 | 30 |
| Test 3 | Common feed | Examples1 composite feed additive | 1.5 | 10 |
| Test 4 | Common feed | Example 2 Compound feed additive | 1.5 | 20 |
| Test 5 | Common feed | Example 3 Compound feed additive | 1.5 | 20 |
| Test 6 | Common feed | Example 4 Compound feed additive | 1.5 | 20 |
| Test 7 | Common feed | EXAMPLE 5 Compound feed additive | 1.5 | 40 |
| Test 8 | Common feed | Example 6 Compound feed additive | 1.5 | 40 |
| Test 9 | Common feed | Example 7 Compound feed additive | 1.5 | 40 |
| Test 10 | Common feed | Example 8 Compound feed additive | 1.5 | 30 |
| Test 11 | Common feed | Example 9 Compound feed additive | 1.5 | 30 |
| Test 12 | Common feed | Example 10 Compound feed additive | 1.5 | 30 |
From the results, in the effect of preventing the diarrhea of the piglets, although the single traditional Chinese medicine component or the chitosan quaternary ammonium salt has a certain improvement effect on the diarrhea rate of the piglets, the diarrhea rate of the piglets is still high; the compound feed additive has obvious effect on the diarrhea rate of piglets, and compared with a control group, the diarrhea rate of the piglets added with the compound feed additive is reduced by at least 60 percent. When chitosan quaternary ammonium salt with various molecular weights is added aiming at the added chitosan quaternary ammonium salt in the compound feed additive, the improvement effect on the diarrhea rate of the young animals is better than that of the added chitosan quaternary ammonium salt with single molecular weight; when 3 kinds of chitosan quaternary ammonium salt with molecular weight are added, the improvement effect on the piglet diarrhea rate is most obvious, the piglet diarrhea rate is reduced by at least 30% and 10% compared with the piglet diarrhea rate only added with traditional Chinese medicine components and only added with chitosan quaternary ammonium salt, and the effect is better than other concentrations when the addition amount of the feed additive accounts for 1.5% of the total amount of the feed.
The results show that the components in the compound feed additive have a synergistic effect, the effect is better than that of the single addition of the traditional Chinese medicines or the chitosan quaternary ammonium salt, the effect is better than that of other concentrations when the addition amount of the feed additive accounts for 1.5 percent of the total amount of the feed, the effect of compounding the chitosan quaternary ammonium salt mixed by the traditional Chinese medicines and 3 molecular weights in the embodiment 1 as the feed additive is the best, and the effect of preventing and treating the diarrhea of piglets is the most obvious.
2. Application in adult pigs
(1) The test method was the same as that of the experimental example of weaned pigs except that the test subjects were adult pigs.
(2) And (3) test results:
| group of | Feed stuff | Feed additive | Feed additive mass ratio (%) | Diarrhea Rate (%) |
| Blank control group | Common feed | - | - | Without change |
| Common control | Common feed | - | 0 | 90 |
| Comparative example 1 | Common feed | The herbal composition of example 1 | 1.5 | 60 |
| Comparative example 2 | Common feed | The herbal composition of example 2 | 1.5 | 60 |
| Comparative example 3 | Common feed | The Chinese medicinal composition of example 3 | 1.5 | 60 |
| Comparative example 4 | Common feed | Example 4 Chinese medicinal Components | 1.5 | 60 |
| Comparative example 5 | Common feed | Examples1 Chitosan Quaternary ammonium salt | 1.5 | 50 |
| Comparative example 6 | Common feed | Quaternary ammonium salt of chitosan of example 2 | 1.5 | 60 |
| Comparative example 7 | Common feed | Quaternary ammonium salt of chitosan of example 3 | 1.5 | 60 |
| Comparative example 8 | Common feed | Quaternary ammonium salt of chitosan of example 4 | 1.5 | 60 |
| Test 1 | Common feed | Example 1 Compound feed additive | 1 | 30 |
| Test 2 | Common feed | Example 1 Compound feed additive | 2 | 30 |
| Test 3 | Common feed | Example 1 Compound feed additive | 1.5 | 10 |
| Test 4 | Common feed | Example 2 Compound feed additive | 1.5 | 20 |
| Test 5 | Common feed | Example 3 Compound feed additive | 1.5 | 20 |
| Test 6 | Common feed | Example 4 Compound feed additive | 1.5 | 20 |
| Test 7 | Common feed | EXAMPLE 5 Compound feed additive | 1.5 | 40 |
| Test 8 | Common feed | Example 6 Compound feed additive | 1.5 | 40 |
| Test 9 | Common feed | Example 7 Compound feed additive | 1.5 | 40 |
| Test 10 | Common feed | Example 8 Compound feed additive | 1.5 | 30 |
| Test 11 | Common feed | Example 9 Compound feed additive | 1.5 | 30 |
| Test 12 | Common feed | Example 9 Compound feed additive | 1.5 | 30 |
From the results, in the effect of preventing the diarrhea of the adult pigs, although the single traditional Chinese medicine component or the chitosan quaternary ammonium salt has a certain improvement effect on the diarrhea rate of the piglets, the diarrhea rate of the piglets is still high; the compound feed additive has obvious effect, and compared with a control group, the diarrhea rate of the adult pigs added with the compound feed additive is reduced by at least 50%. When chitosan quaternary ammonium salt with various molecular weights is added aiming at the added chitosan quaternary ammonium salt in the compound feed additive, the improvement effect on the diarrhea rate of the young animals is better than that of the added chitosan quaternary ammonium salt with single molecular weight; when 3 kinds of chitosan quaternary ammonium salt with molecular weight are added, the improvement effect on the piglet diarrhea rate is most obvious, the piglet diarrhea rate is reduced by at least 20% and 10% compared with the piglet diarrhea rate only added with the traditional Chinese medicine components and only added with the chitosan quaternary ammonium salt, and the effect is better than other concentrations when the addition amount of the feed additive accounts for 1.5% of the total amount of the feed.
The results show that the components in the compound feed additive have synergistic effect, and the effect is better than that of singly adding traditional Chinese medicine components or chitosan quaternary ammonium salt; when the traditional Chinese medicine components and the chitosan quaternary ammonium salt are used together, the effect of adopting the chitosan quaternary ammonium salt mixed with different molecular weights is better; in the above-listed experimental examples, the effect of the compound feed additive in example 1 was the best, and the effect of preventing and treating diarrhea in piglets and adult pigs was the best.
Claims (9)
1. A chitosan quaternary ammonium salt and Chinese herbal medicine compound feed additive is characterized in that the compound feed additive comprises traditional Chinese medicine components and chitosan quaternary ammonium salt; the traditional Chinese medicine comprises the following raw materials in parts by mass: 10-30 parts of dried orange peel, 40-60 parts of astragalus membranaceus, 30-50 parts of radix isatidis, 20-40 parts of houttuynia cordata, 20-40 parts of pomegranate rind, 10-30 parts of radish seed, 15-35 parts of radix bupleuri, 20-40 parts of coptis chinensis and 8-20 parts of angelica sinensis; the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 1-3;
the chitosan quaternary ammonium salt is prepared from chitosan quaternary ammonium salts with molecular weights of 800-1200, 2000-2500 and 40000-60000 according to a mass ratio of 1-3: 1-3: 1 to 3.
2. The compound feed additive according to claim 1, wherein the traditional Chinese medicine components comprise the following raw materials in parts by mass: 15-25 parts of dried orange peel, 45-55 parts of astragalus membranaceus, 35-45 parts of radix isatidis, 25-35 parts of houttuynia cordata, 25-35 parts of pomegranate rind, 15-25 parts of radish seed, 20-30 parts of radix bupleuri, 25-35 parts of coptis chinensis and 10-18 parts of angelica sinensis.
3. The compound feed additive according to claim 2, wherein the traditional Chinese medicine components comprise the following raw materials in parts by mass: 20 parts of dried orange peel, 50 parts of astragalus, 40 parts of isatis root, 30 parts of houttuynia, 30 parts of pomegranate bark, 20 parts of radish seed, 25 parts of radix bupleuri, 30 parts of coptis root and 15 parts of angelica.
4. The compound feed additive as claimed in claim 1, wherein the chitosan quaternary ammonium salt is prepared from chitosan quaternary ammonium salt with molecular weight of 900-1100, 2100-2300, 45000-55000 according to mass ratio of 1-2: 1-2: 1 to 2.
5. The compound feed additive as claimed in claim 4, wherein the chitosan quaternary ammonium salt is prepared from chitosan quaternary ammonium salts with molecular weights of 1000, 2200 and 50000 according to a mass ratio of 1:1: 1.
6. The compound feed additive according to claim 1, wherein the mass ratio of the chitosan quaternary ammonium salt to the traditional Chinese medicine components is 1: 2.
7. the preparation method of the compound feed additive as claimed in any one of claims 1 to 6, characterized in that, the Chinese medicinal materials in the formula are selected, impurities and non-medicinal parts of the Chinese medicinal materials are respectively removed, the water content of the medicinal parts is ensured to be lower than 10%, and the Chinese medicinal materials are mixed, crushed and sieved by a 100-200 mesh sieve according to the formula amount; then evenly mixing the mixture with chitosan quaternary ammonium salt and drying to obtain the compound feed additive.
8. The use of the compound feed additive of any one of claims 1 to 6 in the preparation of a pig feed, wherein the compound feed additive is added to the pig feed in an amount of 1 to 2% by weight of the total amount.
9. The use according to claim 8, wherein the compound feed additive is added to the pig feed in an amount of 1.5% by mass of the total mass.
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