CN108576537A - 一种秋葵酵素的制作方法 - Google Patents
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Abstract
本发明公开了一种秋葵酵素的制作方法,涉及秋葵酵素技术领域,该制作方法以由秋葵果实和花混合的秋葵混料以及秋葵蜂蜜为原料,采用直接发酵工艺,整个制作过程操作简单,无需昂贵、复杂的设备,且发酵温度在25‑30℃,能耗小,复合绿色工业生产的理念,制备的秋葵酵素液具有助消化,润肠通便、解酒护肝、调解血脂平衡、降低高胆固醇血症等作用。
Description
技术领域
本发明属于保健饮品技术领域,涉及秋葵酵素技术领域,具体涉及一种秋葵酵素的制作方法。
背景技术
黄秋葵简称秋葵,锦葵科、属一年生草本植物,美国人称其为“植物伟哥”,日本人称之为“绿色人参”,富含果胶及多糖组成的黏性物质,其中可溶性膳食纤维可以促进消化和胃肠道蠕动,改善便秘;锌、硒等微量元素,有抗氧化,抗衰老的功效,也有益于抗肿瘤。长期食用黄秋葵,有护肤、健胃、保肝、强肾等功效,口感爽滑,其叶片、芽、花等亦可食用,是一种复合现代营养理念的高档绿色保健蔬菜。酵素称为酶,是一类生物催化剂,是生命的原动力,也是身体的清道夫,大多数酵素由蛋白质构成。酵素几乎参与所有的生理活动,使人体能维持正常的机体免疫、新陈代谢、修复组织等生理功能。由于酵素产品是最近几年才逐步进行人们视线的,因此关于秋葵酵素的研究虽有相关的报道出现,如中国专利公开号CN105533539A以秋葵、红糖和柠檬为原料发酵制得秋葵酵素,具有制备周期短,口感好的优点,同样中国专利公开号CN105167096A采用秋葵、干东革阿里、蜜糖、寡糖和干酵母粉采用纯植物发酵制得秋葵酵素营养液,但目前仍处于初步阶段,且大多发酵步骤较为繁琐,或选材昂贵,不利于秋葵酵素的工业化发展。
发明内容
根据以上现有技术的不足,本发明所要解决的技术问题是提出一种秋葵酵素的制作方法,为了解决上述技术问题,本发明采用的技术方案为:
一种秋葵酵素的制作方法,所述制作方法的具体步骤如下:将精选的秋葵嫩果和秋葵花清洗干净,先将秋葵嫩果切片,然后将秋葵嫩果片和秋葵花加入发酵罐,再加入红糖和秋葵蜂蜜,混合均匀后,在温度25-30℃密封发酵45-60天,发酵过程中每7-8天排气一次,18-20天翻料一次,发酵液的pH为4-5时结束发酵,无菌过滤,得秋葵酵素。
优选的,为了降低秋葵酵素产品的生产制造成本,选材简单易得,扩大秋葵产业链的发展,保证酵素的口味,所述秋葵酵素包括如下质量百分数成份:秋葵嫩果片50-65%,秋葵花30-40%,红糖5-10%,秋葵蜂蜜1-1.5%。
优选的,所述秋葵酵素包括如下质量百分数成份:秋葵嫩果片60%,秋葵花30%,红糖8.5%,秋葵蜂蜜1.5%。
优选的,为了提高原料的利用率,发酵程度的完全,所述秋葵嫩果片厚度为0.5-0.8mm,表面喷涂有活性酵母液,喷涂量为0.5-1kg/10mL。
优选的,为了提高发酵速率,所述活化酵母液的制备方法如下:将0.5-0.8g活性酵母加入100-150mL的由质量比2:0.5-1的0.02g/mL葡萄糖和秋葵多糖混合的溶液中,在30-35℃活化20-25min。
与现有技术相比,本发明的有益效果:
1.本发明采用秋葵的果实和花作为原料,并添加秋葵蜂蜜,不仅扩大秋葵产业链的发展,并将秋葵的药用保健价值最大化,制备出的秋葵酵素具有助消化,润肠通便、解酒护肝、调解血脂平衡、降低高胆固醇血症作用。
2.本发明采用直接发酵方法进行发酵,具有制备方法简单易操作,降低秋葵酵素生产成本,有利于秋葵酵素的工业化生产。
具体实施方式
下面通过对实施例的描述,作进一步详细的说明,以帮助本领域技术人员对本发明的发明构思、技术方案有更完整、准确和深入的理解。
实施例1
本实施例中秋葵酵素的制作方法,具体步骤如下:挑取果实饱满无虫害的健康秋葵嫩果,和无虫害待盛开的秋葵花,将精选的秋葵嫩果和秋葵花清洗干净,在清洗的过程中采取轻柔,缓冲的方式,保证选取的秋葵花原料的完整性,将秋葵嫩果切片,嫩果片的厚度在0.5-0.8mm,然后将秋葵嫩果片和秋葵花置于无菌环境下,晾干表面水分,然后取0.8g的活性酵母,将活性酵母加入150mL的由质量比2:0.8的0.02g/mL葡萄糖和秋葵淀粉混合的溶液中在35℃活化20min,将得到的活化酵母液喷洒于晾干表面水分的秋葵嫩果片的表面,喷洒的量为0.8kg/10mL,喷洒后,在室温静止堆放2-3h,然后分别取上述处理后的质量占比如下的秋葵嫩果片60%,秋葵花30%加入发酵罐,再加入质量占比8.5%的红糖和1.5%的秋葵蜂蜜,混合均匀后,在温度30℃密封发酵50天,发酵过程中每7天排气一次,20天翻料一次,待发酵液的pH为4-5时结束发酵,出发酵罐,进行无菌过滤,得秋葵酵素。
此实施例中制备的秋葵酵素液,富含秋葵的清香气味,且气味香醇,无酒精及异味。酵素液呈胶黏状。
实施例2
本实施例同实施例1,不同的是本实施例中秋葵酵素包括如下质量百分数成份:秋葵嫩果片50%,秋葵花40%,红糖9%,秋葵蜂蜜1%,活化酵母液的喷洒量为0.5kg/10mL。
此实施例中制备的秋葵酵素液,口感适宜,红糖的味道稍浓,富含秋葵的清香气味,且气味香醇,无酒精及异味。酵素液呈胶黏状。
秋葵含有的可溶性膳食纤维可以促进消化和胃肠道蠕动,改善便秘;锌、硒等微量元素,有抗氧化,抗衰老的功效,也有益于抗肿瘤。长期食用秋葵,有护肤、健胃、保肝、强肾等功效,制备的秋葵酵素具有助消化,润肠通便、解酒护肝、调解血脂平衡、降低高胆固醇血症等作用。
上面结合具体实施例对本发明进行了示例性描述,显然本发明具体实现并不受上述方式的限制,只要采用了本发明的方法构思和技术方案进行的各种非实质性的改进,或未经改进将本发明的构思和技术方案直接应用于其它场合的,均在本发明的保护范围之内。本发明的保护范围应该以权利要求书所限定的保护范围为准。
Claims (5)
1.一种秋葵酵素的制作方法,其特征在于,所述制作方法的具体步骤如下:将精选的秋葵嫩果和秋葵花清洗干净,先将秋葵嫩果切片,然后将秋葵嫩果片和秋葵花加入发酵罐,再加入红糖和秋葵蜂蜜,混合均匀后,在温度25-30℃密封发酵45-60天,发酵过程中每7-8天排气一次,18-20天翻料一次,发酵液的pH为4-5时结束发酵,无菌过滤,得秋葵酵素。
2.根据权利要求1所述的秋葵酵素的制作方法,其特征在于,所述秋葵酵素包括如下质量百分数成份:秋葵嫩果片50-65%,秋葵花30-40%,红糖5-10%,秋葵蜂蜜1-1.5%。
3.根据权利要求1所述的秋葵酵素的制作方法,其特征在于,所述秋葵酵素包括如下质量百分数成份:秋葵嫩果片60%,秋葵花30%,红糖8.5%,秋葵蜂蜜1.5%。
4.根据权利要求3所述的秋葵酵素的制作方法,其特征在于,所述秋葵嫩果片厚度为0.5-0.8mm,表面喷涂活化酵母液,喷涂量为0.5-1kg/10mL。
5.根据权利要求4所述的秋葵酵素的制作方法,其特征在于,所述活化酵母液的制备方法如下:将0.5-0.8g活性酵母加入100-150mL的由质量比2:0.5-1的0.02g/mL葡萄糖和秋葵多糖混合的溶液中,在30-35℃活化20-25min。
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104996997A (zh) * | 2015-06-29 | 2015-10-28 | 武汉葵锦生物科技有限公司 | 一种黄秋葵复合益生菌酵素粉的制备方法 |
CN105167096A (zh) * | 2015-11-02 | 2015-12-23 | 广西金臣科技有限公司 | 秋葵酵素营养液及其制备方法 |
CN105232441A (zh) * | 2015-10-31 | 2016-01-13 | 代秀梅 | 一种酵素祛斑膏的制备方法 |
CN105533539A (zh) * | 2015-12-24 | 2016-05-04 | 广西良樱农业科技有限公司 | 黄秋葵酵素的制作方法 |
CN107156810A (zh) * | 2017-04-28 | 2017-09-15 | 邓和满 | 果蔬酵素及其制备方法 |
CN107950700A (zh) * | 2018-01-09 | 2018-04-24 | 广东东方翠健康科技有限公司 | 茶酵素饮料及其制备方法 |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104996997A (zh) * | 2015-06-29 | 2015-10-28 | 武汉葵锦生物科技有限公司 | 一种黄秋葵复合益生菌酵素粉的制备方法 |
CN105232441A (zh) * | 2015-10-31 | 2016-01-13 | 代秀梅 | 一种酵素祛斑膏的制备方法 |
CN105167096A (zh) * | 2015-11-02 | 2015-12-23 | 广西金臣科技有限公司 | 秋葵酵素营养液及其制备方法 |
CN105533539A (zh) * | 2015-12-24 | 2016-05-04 | 广西良樱农业科技有限公司 | 黄秋葵酵素的制作方法 |
CN107156810A (zh) * | 2017-04-28 | 2017-09-15 | 邓和满 | 果蔬酵素及其制备方法 |
CN107950700A (zh) * | 2018-01-09 | 2018-04-24 | 广东东方翠健康科技有限公司 | 茶酵素饮料及其制备方法 |
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