CN108478518A

CN108478518A - A kind of composition and the acne-eliminating cosmetic containing the composition

Info

Publication number: CN108478518A
Application number: CN201810265891.1A
Authority: CN
Inventors: 戴跃锋; 康文术; 何广文; 颜少慰
Original assignee: HUNAN YUJIA COSMETICS MANUFACTURING Co Ltd
Current assignee: HUNAN YUJIA COSMETICS MANUFACTURING Co Ltd
Priority date: 2018-03-28
Filing date: 2018-03-28
Publication date: 2018-09-04

Abstract

The present invention relates to cosmetic field, more particularly to a kind of composition and the acne-eliminating cosmetic containing the composition.Including hydroxyacetic acid, Bacillus acidi lactici/pear juice tunning filtrate, Huang Drug bark extracts, white willow bark extract, Chinese glutinous rehmannia root extract, Neem leaf extract, Wild soybean extract, niacinamide, capriloyl glycine, kuh-seng root extract, Salvia root P.E, silandiol salicylate, zinc pyrrolidone carboxylate, Quercetin, L magnesium ascorbyl phosphate, Gotu Kola P.E and menthoxy propylene glycol.Composition and cosmetics containing the composition have effects that inhibit to induce, inhibit inflammation, promote healing.

Description

A kind of composition and the acne-eliminating cosmetic containing the composition

Technical field

The present invention relates to cosmetic field, more particularly to a kind of composition and the acne-eliminating cosmetic containing the composition.

Background technology

Acne is a kind of related hair follicle of and endocrine function imbalance, sebaceous glands chronic inflammatory skin, is dept. of dermatology Common disease and frequently-occurring disease, clinical manifestation be facial area hoary hair or blackhead, papule, useless fellow, tubercle, tumour, recess or Hyperplastic scar etc. seriously affects the quality of life of patient.The pathogenesis of acne may increase with smegma, follicular orifice Epithelium hyperkeratinization, the active or internal Serum Testosterone Levels of propionibacterium acnes increase related.Acne clinical onset in puberty, She will influence personal appearance and self-respect, or even cause disease at heart, lead to life sub-health state.Incidence of acne is high, grinds Hair finds that it is generally happened in 16-19 Sui boy and 14-17 Sui girl, high in not agnate 12-24 Sui juvenile onset rate Up to 85%, although acne influences most of to be teenager, it is still accounted in generally existing, such as 20-30 Sui range in adult 40-50%, or even 40 years old or more the people of report 10-20% also suffer from acne.

Since patients with acne is more with puberty men and women, appearance can often be had an impact, and the crowd more focuses on oneself Appearance image, therefore acne generates more serious negative effect to the psychology and quality of life of patient, causes patient's normal life, Study, work, marriage, social communication, mood, self-confidence etc. are severely impacted.Domestic and international result of study shows that acne can make Patient produces a feeling low, anxiety, depression, the undesirable mood such as stress, and therefore leads to the problem of serious social mentality. Most commonly self-esteem, self-confidence is impaired, generates awkward, depression, and nervous psychology leads to patient human communication disorders and influences to learn It practises and works, patient is resulted even in when serious and generates introgression.

As Clinical Medicine Model is from simple biomedical model to biology-society-psychological medicine Mode change, Cuo Sore is not only the disease of simple physiology reward, it has been known as a kind of disease seriously affecting individual psychology health, can influence patient Appearance, emotion is social, interpersonal relationships, and employment etc. causes patient's willpower to weaken, and generates suicide psychology, anxiety suppression It is strongly fragrant, the symptoms such as absent-minded.

It is main following for the nursing of the external used medicine of acne at present：

2.1 Tretinoin

Tretinoin medicines external curing acne, can eliminate sebaceous glands blocking, and colleague reduces the flora of propionibacterium acnes Quantity.But local use Tretinoin can cause adverse reaction, stimulation of the most common side effect to skin, including drying, erythema, Shouting pain, itch etc..

2.1 benzoyl peroxide

Benzoyl peroxide is a species specific microbial inhibitor, especially to propionibacterium acnes and golden yellow grape Coccus has public security degree selectivity, can be used for inhibiting the microorganism in hair follicle in sebaceous glands and kills propionic acid Propiobacterium, to The star for improving inflammation and non-inflammatory skin lesion is mad.But since this ingredient is big to skin irritation, limit its application.

2.3 optical dynamic therapy

Photodynamics is a kind of combination light, photosensitizer and the interior molecular oxygen of tissue, passes through photodynamics and reacts generation active oxygen And the therapy of selective injury target tissue.

Human body to propionibacterium acnes generate porphyrin light absorption top be blue light (415nm), tissue penetration compared with It is weak, therefore blue light mainly for light moderate there is the patients with acne of cutaneous inflammatory lesion to have preferable therapeutic effect.Feux rouges (633nm) has Have stronger tissue penetration, can not only stimulating expression of macrophage discharge cell factor, reach certain anti-inflammatory effect, can also activate Fibroblast in cell generates growth factor, and the new collagen of induced expression promotes the reparation to injury tissue, therefore, feux rouges It is preferable to profound skin lesion therapeutic effect.

Intense pulsed light

Principle used in IPL be selective light pyrolysis, common wavelength 530-750nm, target tissue be mainly melanin and Hemoglobin, they are heated after absorbing photon, are destroyed, are finally removed by immunocyte.IPL in clinical application in scar, Inflammation, pigmented treatment, and with wound it is small, restore it is fast a little.

Using photodynamic therapy acne, mainly for bacterial destruction, inflammation is eliminated, Shortcomings, single therapy cost Height, treatment cycle is long, and needs monthly to treat its 4-6 month into behavior to hospital or professional institution.

2.4 antibiotic

Erythromycin, chloramphenicol, clindamycin can be configured to the externally applied drug of suitable concentration with alcohol or propylene glycol, although note that The drug resistance problems of antibiotic should cautiously use antibiotic.Topical antibiotic can cause skin office as topical Retinoids Portion is red and swollen, dry decortication and pruritis.

2.5 estrogen

Women with Acne can be used the treatment of anti-male technique, and medicine includes contraceptive, spirolactone, and western miaow replaces Fourth.Contraceptive is made of ethinylestradiol and cyproterone, is the most common drug of antiandrogen.Studies have shown that contraceptive joins With the more ovum syndromes of hypoglycemic agent Or Metformin In Treating, as a result surface treatment scheme can improve Hyperandrogenism symptom, reduce Ovarian Volume, but long-time service contraceptive can influence body glycometabolism, cause hyperinsulinemia.

The alternative destruction sexual gland of spirolactone and adrenal cytochrome P 450 Enzyme system, inhibit androgen to generate enzyme Activity reduces the generation of androgen.But spirolactone will produce potassemia, and kidney function damage and gastrointestinal reaction etc. are bad anti- It answers.

Cimetidine has weaker anti-androgenic effect, competitive DHT can be blocked to be combined with receptor, but do not influence serum Androgen levels.But data acne is not obvious, and with abalienation, the adverse reactions such as fever of having sore throat.

2.5 care product

Most of products for whelk nursing, the overwhelming majority rest on cleaning oil-control, chemical stripping cutin on the market Application, for propionibacterium acnes induce inflammation, cell cytokine release, induction immune response angle inhibit acne Caused tissue damage and destruction.Market poor quality cosmetics, add hormonal components in formula, serious to destroy consumer skin's screen Barrier, the problem of leading to hormone face skin.

Therefore it provides a kind of inhibit to induce, inflammation, the composition for promoting healing and cosmetics inhibited to have important reality Meaning.

Invention content

In view of this, the present invention provides a kind of composition and the acne-eliminating cosmetic containing the composition.The composition and contain There are the cosmetics of the composition to have effects that inhibit to induce, inhibit inflammation, promote healing.

In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：

The present invention provides a kind of compositions, including hydroxyacetic acid, Bacillus acidi lactici/pear juice tunning filtrate, Huang Drug trees Bark extract, white willow bark extract, Chinese glutinous rehmannia root extract, Neem leaf extract, Wild soybean extract, nicotinoyl Amine, capriloyl glycine, kuh-seng root extract, Salvia root P.E, silandiol salicylate, zinc pyrrolidone carboxylate, quercitrin Element, Magnesium L-Ascorbyl Phosphate, Gotu Kola P.E and menthoxy propylene glycol.

In some specific embodiments of the present invention, in terms of mass parts, the composition includes：

In some specific embodiments of the present invention, the composition further includes glycerine, propylene glycol, butanediol, oligomerization Hyaluronic acid, beta glucan, glycine betaine, allantoin, arginine, PEG-60 rilanit specials, Sensiva SC50, glycerine octanoic acid Mixture more than one or both of ester, α-cymene -5- alcohol, radix scutellariae root extract, houttuynia extract.

Wherein, based on mass fraction, the group of the acne jinx becomes：

The present invention also provides the compositions to prepare inhibition sebaceous glands proliferation and activity, and sebum is inhibited excessively to divide It secretes, dredging inhibits propionibacterium acnes activity, slow down propionic acid acne bar because sebaceous glands gland conduit hyperkeratosis causes clogging of pores Inflammation and immune response that free fatty caused by sebum and exoenzyme induce are decomposed, free radical is eliminated, repairs because of inflammation Caused acne skin skin lesion slows down because acne inflammation, immune response lead to cutaneous pigmentation, recess and scar and/or repair The drug of multiple skin barrier function and/or the application in cosmetics.

The present invention also provides application of the composition in preparing treatment and/or preventing acne.

The present invention also provides acceptable auxiliary materials in a kind of cosmetics, including composition as mentioned and cosmetics.

In some specific embodiments of the present invention, based on mass fraction, the cosmetics include：

Wherein, based on mass fraction, the group of the acne jinx becomes：

The present invention some specific embodiments in, the cosmetics be anti-acne mildy wash, acne removing water, anti-acne essence, Whelk-eliminating paste or Deacne pack.Within protection scope of the present invention, the present invention is herein for acceptable dosage form in every cosmetics It does not limit.

The present invention also provides the preparation methods of the cosmetics, include the following steps：

Step 1：Weigh deionized water；

Step 2：Respectively weigh glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, Niacinamide, silandiol salicylate, zinc pyrrolidone carboxylate, capriloyl glycine and arginine, are heated to 75~80 DEG C, with 150~200r/min stirs 30min, with 6000~7000r/min homogeneous 3min；

Step 3：Magnesium L-Ascorbyl Phosphate is weighed respectively, xanthans is cooled to 60~75 DEG C of additions, with 200~ 250r/min stirs 30min, with 8000~9000r/min homogeneous 3min；

Step 4：Reduce stir speed (S.S.) to 150~200r/min, temperature is down to 45~55 DEG C, weigh kuh-seng root extract, Salvia root P.E, Quercetin, Gotu Kola P.E and acne jinx stir 20~30min；

Step 5：Temperature reduces by 45~50 DEG C, weighs preservative PHL and menthoxy propylene glycol, stirring 10min coolings.

The present invention also provides cosmetics made from the cosmetics or preparation method as mentioned to prepare inhibition skin Adipose gland, which is proliferated, to be dredged because sebaceous glands gland conduit hyperkeratosis causes clogging of pores with activity, inhibition sebum excessive secretion, inhibits Cuo Sore Propionibacterium activity slows down propionic acid acne bar and decomposes the inflammation and exempt from that free fatty caused by sebum and exoenzyme induce Free radical is eliminated in epidemic disease response, is repaired the acne skin skin lesion caused by inflammation, is slowed down because acne inflammation, immune response lead to skin Skin pigmentation, recess and scar and/or the drug for repairing skin barrier function and/or the application in cosmetics.

The present invention also provides cosmetics made from the cosmetics or preparation method as mentioned prepare treatment and/ Or the application in prevention acne.

The present invention provides formula for dispelling pox, including hydroxyacetic acid, Bacillus acidi lactici/pear juice tunning filtrate, Huang Drug barks Extract, white willow bark extract, Chinese glutinous rehmannia root extract, Neem leaf extract, Wild soybean extract, niacinamide, Capriloyl glycine, kuh-seng root extract, Salvia root P.E, silandiol salicylate, zinc pyrrolidone carboxylate, Quercetin, Magnesium L-Ascorbyl Phosphate, Gotu Kola P.E and menthoxy propylene glycol.From oil-control, sterilizing, anti-inflammatory, four dimensions are repaired Carry out scientific formulation compatibility and evaluation.

Acne is caused skin problem to carry out work(stage by stage respectively with induction stage, inflammation phase, healing phases by the present invention Imitate target spot control.By inhibiting sebaceous glands proliferation and activity, inhibits sebum excessive secretion, dredge because of the excessive angle of sebaceous glands gland conduit Change causes clogging of pores, inhibits propionibacterium acnes activity, slow down propionic acid acne bar decompose free fatty caused by sebum and The inflammation and immune response that exoenzyme induces eliminate free radical, repair the acne skin skin lesion caused by inflammation, slow down because of Cuo Sore inflammation, immune response lead to cutaneous pigmentation, recess and scar, repair skin barrier function.

The present invention is widely used in the relevant product of skin care, and such as anti-acne mildy wash, acne removing water, anti-acne essence is dispelled Acne cream, in the products such as Deacne pack.

Description of the drawings

In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technology description to be briefly described.

Fig. 1 shows that skin oil and fat secretes testing result；

Fig. 2 shows that moisture of skin scatters and disappears testing result；

Fig. 3 shows nitric oxide releasing testing result；

Fig. 4 is shown as fibrocyte proliferation testing result；

Fig. 5 shows Propiobacterium clump count testing result；

Fig. 6 shows cell melanin content detection result；

Fig. 7 shows tester's face acnes cure rate result；Wherein, figure A~figure D shows high-resolution (CANON zoom LENS EF-S/18-55mm/ φ 58mm) camera take pictures as a result, photographing data be respectively 2017-05-08,2017-05-15, 2017-05-08、2017-06-05；Figure E~figure H shows that 3D rendering, date are respectively 2017-05-08,2017-05-15,2017- 05-08、2017-06-05。

Specific implementation mode

The invention discloses a kind of composition and containing the acne-eliminating cosmetic of the composition, those skilled in the art can borrow Reflect present disclosure, is suitably modified technological parameter realization.In particular, it should be pointed out that all similar substitutions and modifications are to this field It is it will be apparent that they are considered as being included in the present invention for technical staff.The method of the present invention and application have passed through Preferred embodiment is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to described herein Methods and applications be modified or suitably change and combine, to realize and apply the technology of the present invention.

Raw materials used, auxiliary material and reagent in composition provided by the invention and acne-eliminating cosmetic containing the composition It is bought by market.

Wherein, acne jinx is purchased from a ball company；

Heretofore described acne jinx is at being grouped as：

Component	Match %
		Water	46.7975
Butanediol	40.3755
		Hydroxyacetic acid	5.6
PEG-60 rilanit specials	4
		Sensiva SC50	1
Glycerol caprylate	1
		Bacillus acidi lactici/pear juice tunning filtrate	0.7
α-cymene -5- alcohol	0.25
		Yellow Drug bark extracts	0.125
White willow bark extract	0.05
		Chinese glutinous rehmannia root extract	0.04
Neem leaf extract	0.02
		Radix scutellariae root extract	0.018
Houttuynia extract	0.016
		Wild soybean extract	0.008

With reference to embodiment, the present invention is further explained：

Embodiment 1

Xanthans 0.2%, PHL 1% are added, residue addition deionized water to 100% prepares anti-acne water component and carries out work( Effect evaluation, Recipe are as follows：

1) its deionized water is weighed according to processing compound；

2) glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, nicotinoyl are weighed respectively Amine, silandiol salicylate, zinc pyrrolidone carboxylate, capriloyl glycine and arginine, are heated to 75 DEG C, with 180r/ Min stirs 30min, with 6000r/min homogeneous 3min；

3) Magnesium L-Ascorbyl Phosphate is weighed respectively, and xanthans is cooled to 68 DEG C of additions, with 200r/min, stirring 30min, with 8500r/min homogeneous 3min；

4) stir speed (S.S.) is reduced to 150r/min, and temperature is down to 50 DEG C, weighs kuh-seng root extract, Radix Salviae Miltiorrhizae extraction respectively Object, Quercetin, Gotu Kola P.E and acne jinx stir 20min；

5) temperature reduces by 45 DEG C, weighs preservative PHL and menthoxy propylene glycol, and stirring 10min cools down room temperature.

Embodiment 2

1) its deionized water is weighed according to processing compound；

2) glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, nicotinoyl are weighed respectively Amine, silandiol salicylate, zinc pyrrolidone carboxylate, capriloyl glycine and arginine, are heated to 80 DEG C, with 200r/ Min stirs 30min, with 7000r/min homogeneous 3min；

3) Magnesium L-Ascorbyl Phosphate is weighed respectively, and xanthans is cooled to 75 DEG C of additions, with 250r/min, stirring 30min, with 9000r/min homogeneous 3min；

4) stir speed (S.S.) is reduced to 200r/min, and temperature is down to 55 DEG C, weighs kuh-seng root extract, Radix Salviae Miltiorrhizae extraction respectively Object, Quercetin, Gotu Kola P.E and acne jinx stir 30min；

5) temperature reduces by 50 DEG C, weighs preservative PHL and menthoxy propylene glycol, and stirring 10min cools down room temperature.

Embodiment 3

Glycerine	5%
		Propylene glycol	4%
Butanediol	5%
		Oligomerization hyaluronic acid	0.1%
Beta glucan	0.5%
		Glycine betaine	0.8%
Allantoin	0.2%
		Niacinamide	5%
Capriloyl glycine	1%
		Arginine	1%
Acne jinx	2%
		Kuh-seng root extract	0.5%
Salvia root P.E	0.5%
		Silandiol salicylate	1%
Zinc pyrrolidone carboxylate	0.1%
		Quercetin	0.02%
Magnesium L-Ascorbyl Phosphate	3%
		Gotu Kola P.E	0.5%
Menthoxy propylene glycol	0.1%

1) its deionized water is weighed according to processing compound；

2) glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, nicotinoyl are weighed respectively Amine, silandiol salicylate, zinc pyrrolidone carboxylate, capriloyl glycine and arginine, are heated to 78 DEG C, with 150r/ Min stirs 30min, with 6500r/min homogeneous 3min；

3) Magnesium L-Ascorbyl Phosphate is weighed respectively, and xanthans is cooled to 60 DEG C of additions, with 230r/min, stirring 30min, with 8000r/min homogeneous 3min；

4) stir speed (S.S.) is reduced to 180r/min, and temperature is down to 45 DEG C, weighs kuh-seng root extract, Radix Salviae Miltiorrhizae extraction respectively Object, Quercetin, Gotu Kola P.E and acne jinx stir 25min；

5) temperature reduces by 40 DEG C, weighs preservative PHL and menthoxy propylene glycol, and stirring 10min cools down room temperature.

Comparative example 1

Glycerine	2%
		Propylene glycol	3%
Butanediol	3%
		Oligomerization hyaluronic acid	0.01%
Beta glucan	0.1%
		Glycine betaine	0.5%
Allantoin	0.15%

1) its deionized water is weighed according to processing compound；

2) glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, heating are weighed respectively To 75~80 DEG C, with 150~200r/min, 30min is stirred, with 6000~7000r/min homogeneous 3min；

3) xanthans is weighed, 60~75 DEG C of additions are cooled to, with 200~250r/min, stirs 30min, with 8000~ 9000r/min homogeneous 3min；

4) temperature reduces by 45~50 DEG C, weighs PHL, and stirring 10min cools down room temperature.

Comparative example 2

1) its deionized water is weighed according to processing compound；

2) glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, nicotinoyl are weighed respectively Amine, silandiol salicylate, zinc pyrrolidone carboxylate, capriloyl glycine and arginine, are heated to 75~80 DEG C, with 150 ~200r/min stirs 30min, with 6000~7000r/min homogeneous 3min；

3) Magnesium L-Ascorbyl Phosphate is weighed respectively, and xanthans is cooled to 60~75 DEG C of additions, with 200~250r/ Min stirs 30min, with 8000~9000r/min homogeneous 3min；

4) reduce stir speed (S.S.) to 150~200r/min, temperature is down to 45~55 DEG C, weigh respectively kuh-seng root extract, Salvia root P.E, Quercetin, Gotu Kola P.E and acne jinx stir 20~30min；

5) temperature reduces by 45~50 DEG C, weighs preservative PHL, and stirring 10min cools down room temperature.

Comparative example 3

1) its deionized water is weighed according to processing compound；

2) glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, nicotinoyl are weighed respectively Amine, silandiol salicylate, zinc pyrrolidone carboxylate, capriloyl glycine and arginine are heated to 75~80 DEG C, with 150~ 200r/min stirs 30min, with 6000~7000r/min homogeneous 3min；

Comparative example 4

1) its deionized water is weighed according to processing compound；

5) temperature reduces by 45~50 DEG C, weighs preservative PHL and menthoxy propylene glycol, and stirring 10min coolings room temperature is It can.

4 oil-control efficacy assessments of embodiment

Test philosophy：

The present invention carries out the work(of oil-Absorbing Sheets using effect using the skin oil and fat tester Sebumeter SM815 of Germany CK Effect evaluation.Using universally acknowledged SEBUMETER methods, it is to be based on photometer principle for oil content test, and a kind of 0.1mm is thick After special delustring adhesive tape absorbs grease on human skin, a kind of translucent adhesive tape will be become, its light transmission capacity will be sent out The grease of changing, absorption is more, and light transmission capacity will be bigger, can thus measure the content of skin oil and fat.It is maximum excellent Point is that test probe is small, easy to use, can test any position of skin.This is a kind of indirect survey of grease glandular secretion object As a result amount method can be used for distinguishing different skin types, make accurately to understand the grease as caused by inside and outside reason Variation is possibly realized.

Test method：

The present invention this time tests 21 test objects of selection, and 3 one group of people are divided into 7 groups, and drawing 3 sizes in forehead respectively is The regions 3cm*3cm are directed to blank sample (not wipe samples), control sample and embodiment sample and carry out skin oil and fat content evaluation and test respectively, Each control sample takes the three related judges of value calculating fat content increment percentage progress for each person.

(1) the test object skin health selected, age 18-30 Sui, object is informed after tested, agrees to, study subject is clean Behind face 15 minutes, its forehead fat content is measured as initial value.

(2) do not smear sample in subject's forehead clear area, control sample area, embodiment sample area weigh 0.5ml Essences with After 3cm*3cm non-woven membrane cloth infiltrates cover skin 20min, non-woven fabrics is removed, waits for quietly 10 minutes, is directed to three regions respectively Fat content measurement is carried out, test is primary per 30min, persistently evaluates and tests 2h；

(3) test environment：T=25 DEG C of test environment temperature, humidity H=50；

(4) testing time：Face cleaning is carried out before testing, is tested initial fat content within 15 minutes, is tested 30min respectively, 60min, 90min, 120min test grease content data；

(5) test position：Forehead marks blank, control sample, the test zone of embodiment sample.

Effect evaluation result is as shown in table 1, Fig. 1：

Table 1

Conclusion：The present invention effectively can inhibit skin oil and fat to secrete by evaluation and test, and embodiment sample compares blank sample, in grease Secretion increases low nearly 100% on percentage, and the present invention can effectively reduce sebum excessive secretion, and to cause sebaceous gland duct to block several Rate reduces the possibility that acne occurs.

5 percutaneous moisture loss of embodiment is evaluated

Test philosophy：

The measuring principle of the test apparatus derives from FICK Fick'ss law of diffusion：Dm/dt=D.A.dp/dx.Pass through two groups Temperature-humidity sensor measures the water vapor pressure ladder that nearly epidermis (about in 1cm) is formed by cuticula moisture loss in different bright spots Degree, directly measures the amount of moisture percutaneously distributed.TEWL values are an important symbols of skin barrier quality, and the TEWL values of skin are got over It is low, illustrate that the barrier function of skin is better, on the contrary it is poorer.

(2) experimental condition and volunteer require

Test environment conditions：Test environment temperature is 22 ± 1 DEG C, and humidity is 50 ± 5%, and carries out dynamic in real time and examine It surveys；

Volunteer requires：Effective volunteer at least 30 people, the age, (gestation or the newborn phase women in Pu removed between 16-65 Sui Outside)：Forearm test zone capacitance method moisture of skin measures the basic value of meaning between 15-100：Without serious systemic disease, nothing Immune deficiency or autoimmune disease person；No active anaphylactic disease person；Previously to skin-care cosmetics without severe allergy history Person；Hormone medicine and immunosuppressor person are not used in nearly one month：To participate in other clinical test persons；It uses according to regulations Test medicine and all information；All volunteers should fill in informed consent form before test；

(3) prepare before test

Any product (cosmetics or topical drug) cannot be used within 2-3 days before recipient site.Before experiment, subject needs same It is surveyed in meaning cleaning both hands forearm, with dry face tissue wiped clean.It is surveyed in subject's both hands forearm after cleaning and carries out measured zone Label.At least 30min that sits quietly in room should be being complied with standard before official testing, cannot drunk water, forearm exposure is in test mode It places, keeps loosening.

(4) testing procedure

3*3cm2 test areas are marked in experiment on the inside of left and right arm, same arm can mark multiple regions, interregional Every 1cm.Test product and blank control are randomly dispersed in left and right arm.Tested area is carried out using capacitance method skin analyzer The measurement in domain and control zone.Each region is according to parallel determination 15 times.The plain pape for first measuring each test zone, is then pressed Experiment is spread evenly across in trial zone by the dosage of 2.0 ± 0.1mg samples/cm2 using latex finger cot.Smearing measures 1,2 respectively Hour, 4 hours, the moisture content of skin (being measured by this time when verification) in tested region and blank control region, the same aspiration The test of person is completed by the same survey crew.

Test result is as shown in table 2, Fig. 2：

Empirically design the TEWL values for measuring day part respectively.

TEWL%=(T0-T1)/T0*100%

T0：Use initial skin moisture loss value before sample；

T1：It is scattered and disappeared and is worth using moisture of skin after sample；

Note：Moisture loss value reduces bigger, it is meant that TWEL is smaller, and skin barrier is more intact, concrete numerical value such as following table.

Table 2

Conclusion：Moisture loss reduction of the present invention reaches 17.86%-24.59%, effectively reduces moisture of skin rate of scattering and disappearing, tool Have and repairs skin barrier function well.

Embodiment 6LPS induction RAW264.7 cells discharge NO inhibiting effect

Experimental principle：

LPS (lipopolysaccharides)：

Positioned at the lipopolysaccharides substance of gram-negative bacterial cell wall outermost one layer thicker (8-10nm), by class Fat A, core polysaccharide and 3 part of O- specific side chains composition.Lipopolysaccharides is endotoxin and important group specific antigen (O antigens).Fat Polysaccharide is made of three parts.Lipid A (Lipid A) is the glycolipid for constituting activity of endotoxin, with covalent bonding junction to heteroglycan chain, There are two parts：First, core polysaccharide, is constant in related strain；Another O specificity chain (O-specific chain) is Alterable height.The lipopolysaccharides of Escherichia coli is the i.e. polyclonal work of common B cell mitogeneic factor in Experimental immunization Change the factor (polyclonal activator).This experiment generates allergic reaction using LPS induction RAW264.7 cells, discharges NO (nitric oxide).

Griess assay-NO content test methods

NO is easily oxidized to NO2 in vivo or in aqueous solution, and in acid condition, NO and diazol sulfanilamide (SN) generation diazonium are anti- It answers, and generates diazonium compound, coupled reaction further occurs with naphthylethenyl diamines for the latter, and the product which generates is dense Degree has linear relationship with NO concentration, there is maximum absorption band at 540nm.

Experiment material and method：

Material：DMEM, FBS, LPS, MTT, Griess reagent

Experimental method：

264.7 cell culture of RAW

RAW264.7 cells are drawn into DMEM training bases with liquid-transfering gun and dispel cell dispersion, are counted using Hemacytometer Number cell, is then diluted to a concentration of 5 × 104cells/ml using DMEM come diluting cells.

Cell solution after dilution is inoculated into 96well respectively, and each hole is 100ul, i.e. 5 × 103 cells/well.

It is cultivated 24 hours in 37 DEG C, the incubator of 5%CO2.

Sample to be tested and blank sample prepare：Sample to be tested trains base dilution with DMEM, and the concentration after dilution is respectively：1% (should Concentration is tested by the MTT of front, and result is nontoxic)

Title Control Sample

Sample composition LPS 10ppm LPS 10ppm+1%sample

After cell culture 24 hours, the whether complete adherent growth of cell is observed, it, will if the complete adherent growth of cell Original training base removes, and is washed with DPBS.

After DPBS is removed, it is separately added into the ready sample in front.The solubility of sample is according to the result of toxotest Select safe concentration 1% (20ul/well).

After sample is added, 37 DEG C are put into, is cultivated 20 hours in the incubator of 5%CO2.

It after 20 hours, pipettes on the culture medium to new 96Well of 50ul, 1% Griess of same volume is added Reagent after being sufficiently mixed, reacts 10 minutes in dark place.

Utilize the absorption photometric value of ELISA reader test samples at 540nm.

NO inhibiting effect

NO Inhibition%=((" OD " _ Control- " OD " _ Sample)/" OD " _ Control) * 100%

Wherein：The absorption photometric value of " OD " _ Control- blank samples

The absorption photometric value of " OD " _ Sample- samples

Inhibit inflammatory consequences as shown in Figure 3：

Conclusion：It is 40.98%-46.62% that the present invention, which can effectively inhibit nitric oxide production burst size, for control sample and It is only 14.3% to inhibit nitric oxide releasing amount, and example sample reduces nearly 3 times that nitric oxide releasing amount is control sample, this patent energy Effectively inhibit inflammatory factor, to avoid cascade of response of inflammation, causes skin barrier impaired.

7 fibroblast appreciation rate of embodiment

Experimental principle：

1) method for cell count

Method for cell count is carried out calculating cell suspension systems by white blood cell count(WBC) method using blood cell counting plate After standby, common vital stain trypan blue dyes cell, carries out cell count.It is normally complete that trypan blue cannot penetrate living cells Whole cell membrane, therefore living cells is not colored.And the permeability of cell membrane of dead cell increases, and dyestuff can be made to enter intracellular and make Cell color (blue).

Experiment material and method：

Experiment material:DMEM, DPBS, Typsin-EDTA, Petri dish, 96well dish, 0.4% trypan blue are molten Liquid, absolute ethyl alcohol or 95% ethanol solution, simple microscope, cell counting board, liquid-transfering gun, MTT, DMSO

5.2.3 experimental method：

Fibroblast cell-culture：

1) it is collected after handling cultured fibroblast with Typsin-EDTA, haemocyte is utilized after being suspended with DMEM Suspension is diluted to cell by tally a concentration of 5 × 104cells/ml after counting is spare

2) ready cell suspending liquid is distinguished in plant division to 96well dish and 6well dish and cultivates, wherein 96well dish inoculum concentrations are 100ul, and 6well dish inoculum concentrations are 2ml.

3) it is cultivated 24 hours in 37 DEG C, the incubator of 5%CO2.

Sample adds：

1) sample to be tested trains base dilution with DMEM, and the concentration after dilution is respectively：1% (the MTT surveys that the concentration passes through front Examination, result are nontoxic)

2) after cell culture 24 hours, the DMEM before removing, after then carefully being washed with DPBS

3) the DMEM culture mediums for being added to sample to be tested that the first step prepares are added in sequence.

4) it is cultivated 48 hours in 37 DEG C, the incubator of 5%CO2.

Cell count：

1) tally is handled

After absolute ethyl alcohol or 95% ethanol solution wiping tally, is cleaned with silk, separately clean coverslip one and open, lid Piece overlays on above tally.

2) it dyes

The cell for taking 6well dish to cultivate is digested after removing culture medium using typsin-EDTA, is used after collecting cell DMEM culture mediums suspend.0.4% trypan blue dye liquors of 10ul are drawn with liquid-transfering gun and 10ul cell suspensions are uniformly mixed.From counting Edges of boards edge slowly injects, and is allowed to full of in the gap between tally and cover plate.Tally is placed under low power lens (10 × 10 Times) observation counting.

3) method of counting

By the cell number in the quadrangle block plaid (each block plaid is divided into 16 lattices) of figure count plate.It counts When, complete cell is only counted, is counted by a cell if the cell to bunch up.In a block plaid, if There is cell to be located on line, generally count offline cell and disregard cell of reaching the standard grade, counts left line cell and disregard right line cell.Under the microscope, all Refractivity is by force living cells without tinter, and colors blue person is dead cell.

4) conversion counted

After counting number, the cell number in every ml suspensions is conversed.Since the area of each grid in tally is 0.01cm2, a height of 0.01cm, in this way its volume are 0.0001cm3, i.e. 0.1mm3.Due to 1ml=1000mm3, so often One block plaid inner cell number × 10000=cell numbers/ml, therefore can be calculated as follows：

Cell suspension cell number/ml=4 big lattice total number of cells/4 × 10000

It has been diluted before such as counting, it can be multiplied by extension rate.After counting cell, the dense of cell in cell suspension can be calculated Degree.On the basis of blank sample, the cell proliferation rate of each sample is calculated.

The results are shown in Figure 4 for fibroblast increment：

Conclusion：Cosmetics provided by the invention can example sample promoted fibroblast proliferation up to 36.45%~ 48.6%, and control sample promotes cell proliferation rate down to 16.82%, nearly 2 times of this patent relative comparison sample energy is promoted into fiber finer Born of the same parents are proliferated, and have significant difference (P ＜ 0.05), show that cosmetics provided by the invention have stronger healing for acne lesions Recovery capability.

8 propionic acid Propiobacterium Inhibition test of embodiment

Test evaluating method：

The recovery of propionibacterium acnes and the preparation of bacteria suspension

5% sheep blood is added after improvement GAM agar mediums 115 DEG C of high pressure sterilization 15min, topple over in tablet culture dish, Recovery tablet as freeze-drying bacterial strain.It is inoculated into tablet using plate streak by bacterial strain is lyophilized, 37 DEG C of Anaerobic culturels 72 are small Shi Hou, for the bacterium colony that picking is individually grown to improveing in GAM broth bouillons, dilution is adjusted to 0.5 Maxwell (about than turbid unit 1.5X108CFU/ml)

Bacterium colony counts

It pipettes above-mentioned bacteria suspension 500ul to be added in the sterile test tube of 4500ul physiological saline, obtains the bacterium solution of ten times of dilution. It pipettes in the bacterium solution to the sterile test tube of 4500ul physiological saline of 10 times of 500ul dilutions and obtains the bacterium solution of 102 times of dilution.It is dilute successively The bacterium solution for knowing 108 times of dilution is released, is pipetted on 1ml to plating medium, even spread, 37 DEG C of Anaerobic culturels are right after 72 hours The bacterium colony of growth carries out technology.

The preparation of agar dilution tablet

7 sterile test tubes are taken, 1ml samples are added in each test tube, GAM agar medium heating and meltings will be improved, it is sterile Operation is drawn 9mlGAM agar mediums and is added in above-mentioned each test tube, is poured into tablet after mixing.

Seed agar dilution plate

The bacteria suspension of the various extension rates prepared is pipetted on 1ml to plating medium, even spread, 37 DEG C are detested After oxygen culture 48 hours, the bacterium colony of growth is counted

Propionibacterium acnes inhibition

Tablet is placed in dark-coloured, no-reflection body surface, observes the bacterium colony of propionibacterium acnes, and write down each sample Clump count.

It is as shown in Figure 5 to test evaluation result：

Conclusion：Cosmetics provided by the invention for propionibacterium acnes activity or proliferation by being carried out pair with control sample Than, control sample is for the relatively low inhibiting rate of propionibacterium acnes, and example sample does not find propionibacterium acnes clump count, With significant difference (P ＜ 0.05), show that cosmetics provided by the invention have effects that preferably to inhibit propionibacterium acnes.

The melanin that 9 cell of embodiment generates measures examination

5.6.1 experiment material：B16F10, DMEM culture medium, trypsase, DPBS, 70% alcohol, NaOH, DMSO

5.6.2 experimental method：

(1) by after B16F10 cell dissociations, base cell dispersion is trained using DMEM, is counted using Hemacytometer thin Then born of the same parents are diluted to a concentration of 5 × 104cells/ml using DMEM come diluting cells.

(2) cell solution after diluting is inoculated into 6well respectively, and each hole is 2ml, i.e. 1 × 105cells/well.

(3) it is cultivated 24 hours in 37 DEG C, the incubator of 5%CO2.

(4) sample to be tested prepares：Sample to be tested trains base dilution with DMEM, and the concentration after dilution is respectively：50% (concentration It is tested by the MTT of front, result is nontoxic)

(5) after cell culture 24 hours, the whether complete adherent growth of cell is observed, if the complete adherent growth of cell Original training base is removed, is washed with DPBS by words.

(6) after removing DPBS, it is separately added into the ready sample in front.The solubility of sample is according to the knot of toxotest Fruit selects safe concentration 100ppm (2ml/well).

(7) after sample is added, 37 DEG C is put into, is cultivated 48 hours in the incubator of 5%CO2.

(8) training base and cell are collected respectively, respectively the outer content with intracellular melanin of test cell

(9) training base portion point：After 13500rpm x 10mins centrifugations, supernatant liquor is taken to measure extinction at wavelength 415nm Luminosity.

(10) cellular portions：It will collect after B16F10 cell dissociations, after 13500rpm x 10mins centrifugations, remove Supernatant liquor.The 10%DMSO of 0.3ml is added in the B16F10 cells of bottom, it is small to crack 1 for 1N NAOH solution in 80 DEG C of water-baths When.

Lysate is cooled to room temperature after (11) 1 hours, and supernatant liquor is collected after centrifugation in 13500rpm x 10mins

(12) microplate reader is utilized to measure the absorption photometric OD of supernatant liquor at wavelength 415nm.

(13) melanin standard specimen is configured, a concentration of 1.0 arrive 100.0ppm, test the absorption photometric of standard specimen respectively, draw out The linear relationship chart of concentration and absorption photometric

(14) intracellular and extracellular melanin content can be obtained using above-mentioned linear relationship chart.

5.6.3 experimental result is as shown in table 3, Fig. 6：

Its detailed data is as follows：

Table 3

Cell melanin content (ppm)

Conclusion：Cosmetics example sample provided by the invention inhibits melanin to reach 95.86ppm in the cell, and blank sample is black Pigment content reaches 236.85ppm, relative reduction 140.99ppm；It is 10.42ppm in extracellular example sample black content, it is empty The white outer melanin content of like cell is 20.53ppm, reduces 10.11ppm, and there is the present invention notable (P ＜ 0.05) to inhibit thin The effect of intracellular and extracellular melanin content, to effectively prevent pigmentation caused by acne inflammatory factor.

10 anti-acne clinic exterior syndrome of embodiment is evaluated

Inclusion criteria：

1) meet Pillshury I II grades of acne diagnostic criteria：I grades, facial area, based on acne, a small amount of papule, warts, Total skin lesion<30；II grades, facial area, acne and medium papule, warts, total skin lesion number (30-50) are a；

2) without clear systemic disease；

3) subject during the whole test, does not eat pungent, and sweet food is simultaneously given up alcohol；

4) it tests and other product for resolving poxes or the other therapies (such as tartaric acid, laser therapy etc.) of receiving was not used within first 2 weeks；

5) without using other product for resolving poxes and the other therapies (including phototherapy) of receiving during entire observation test

Exclusion criteria：

1) pregnant, intend gestation, Pu breast phase women；

2) staff of this research is directly participated in；

3) face has other skin diseases, such as chloasma, seborrhea, acne rosacea；

Sampling：

Selection meets I grade, II grades of standard personnel and to each 5 respectively, sooner or later using test sample, carries out on every Mondays VISA carries out face and takes pictures evaluation, continues surrounding, according to cure rate progress efficacy assessments.

With a wherein bit test personnel be its surrounding high power camera take pictures it is as shown in Figure 7 with 3D rendering：

Conclusion：Cosmetics made from the embodiment of the present invention give patients with acne smear, carry out surrounding by a definite date tracking take pictures and 3D skin images fully prove that cosmetics provided by the invention can effectively facilitate that skin barrier caused by whelk is impaired to be obtained It effectively repairs, the cure rate of whelk skin lesion can be promoted.

The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims

1. a kind of composition, which is characterized in that carried including hydroxyacetic acid, Bacillus acidi lactici/pear juice tunning filtrate, Huang Drug barks Take object, white willow bark extract, Chinese glutinous rehmannia root extract, Neem leaf extract, Wild soybean extract, niacinamide, pungent Acylglycine, kuh-seng root extract, Salvia root P.E, silandiol salicylate, zinc pyrrolidone carboxylate, Quercetin, L- Magnesium ascorbyl phosphate, Gotu Kola P.E and menthoxy propylene glycol.

2. composition according to claim 1, which is characterized in that in terms of mass parts, including：

3. composition according to claim 1 or 2, which is characterized in that further include that glycerine, propylene glycol, butanediol, oligomerization are saturating Bright matter acid, beta glucan, glycine betaine, allantoin, arginine, PEG-60 rilanit specials, Sensiva SC50, glycerine octanoic acid Mixture more than one or both of ester, α-cymene -5- alcohol, radix scutellariae root extract, houttuynia extract.

4. composition according to claim 3, which is characterized in that in terms of mass parts, including：

Wherein, based on mass fraction, the group of the acne jinx becomes：

5. composition according to any one of claims 1 to 4 is preparing inhibition sebaceous glands proliferation and activity, inhibit sebum mistake Degree secretion, dredging inhibit propionibacterium acnes activity, slow down propionic acid Cuo because sebaceous glands gland conduit hyperkeratosis causes clogging of pores Sore bar decomposes the inflammation and immune response that free fatty and exoenzyme induce caused by sebum, eliminates free radical, repair because Acne skin skin lesion caused by inflammation, slow down because acne inflammation, immune response cause cutaneous pigmentation, recess and scar and/ Or repair skin barrier function drug and/or the application in cosmetics.

6. application of the composition according to any one of claims 1 to 4 in preparing treatment and/or preventing acne.

7. a kind of cosmetics, which is characterized in that including in such as Claims 1-4 any one of them composition and cosmetics Acceptable auxiliary material.

8. cosmetics according to claim 7, which is characterized in that based on mass fraction, including：

Wherein, based on mass fraction, the group of the acne jinx becomes：

9. the preparation method of cosmetics according to claim 7 or 8, which is characterized in that include the following steps：

Step 1：Weigh deionized water；

Step 2：Glycerine, propylene glycol, butanediol, glycine betaine, allantoin, beta glucan, oligomerization hyaluronic acid, nicotinoyl are weighed respectively Amine, silandiol salicylate, zinc pyrrolidone carboxylate, capriloyl glycine and arginine, are heated to 75~80 DEG C, with 150 ~200r/min stirs 30min, with 6000~7000r/min homogeneous 3min；

Step 3：Magnesium L-Ascorbyl Phosphate is weighed respectively, and xanthans is cooled to 60~75 DEG C of additions, with 200~250r/ Min stirs 30min, with 8000~9000r/min homogeneous 3min；

Step 4：Stir speed (S.S.) is reduced to 150~200r/min, temperature is down to 45~55 DEG C, weighs kuh-seng root extract, Radix Salviae Miltiorrhizae Extract, Quercetin, Gotu Kola P.E and acne jinx stir 20~30min；

10. cosmetics exist made from cosmetics according to claim 7 or 8 or preparation method as claimed in claim 9 It prepares and inhibits sebaceous glands proliferation and activity, sebum excessive secretion, dredging is inhibited to cause pore because of sebaceous glands gland conduit hyperkeratosis It blocks, inhibits propionibacterium acnes activity, slow down free fatty and exoenzyme caused by propionic acid acne bar decomposition sebum and lure The inflammation and immune response of hair eliminate free radical, repair the acne skin skin lesion caused by inflammation, slow down because of acne inflammation, exempt from Epidemic disease response leads to cutaneous pigmentation, recess and scar and/or repairs the drug of skin barrier function and/or answering in cosmetics With.

11. cosmetics exist made from cosmetics according to claim 7 or 8 or preparation method as claimed in claim 9 It prepares treatment and/or prevents the application in acne.