CN107569674B - Anti-allergy relieving composition and application thereof - Google Patents
Anti-allergy relieving composition and application thereof Download PDFInfo
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Abstract
The invention relates to an anti-allergy relieving composition and application thereof, wherein the anti-allergy relieving composition comprises the following components: a first anti-allergy composition, a second anti-allergy composition, and an anti-allergy peptide composition; the active ingredients of the first anti-sensitivity composition comprise 4-tert-butylcyclohexanol and hydroxyphenylpropionamide benzoic acid; the active ingredients of the second antiallergic composition comprise tetrandra root extract, potassium lactate, phenoxyethanol and dipotassium glycyrrhizinate; the active ingredients of the anti-allergy peptide composition comprise palmitoyl tripeptide-8 and acetyl tetrapeptide-15. The anti-allergy relieving composition is evaluated by a macrophage nitric oxide inflammation release model, and the nitric oxide inhibition rate is 38.62%; the histamine inhibition rate reaches 41.92 percent.
Description
Technical field
The present invention relates to technical field of skin care, releive composition and its application more particularly to a kind of antiallergic.
Background technique
Anaphylactia (also referred to as allergic disease) has been not only a kind of common disease, and has become global range
Interior epidemic disease.In fact, being shared in 30 countries epidemiological survey has been carried out in 1,200,000,000 people shown, 2.5 hundred million people (22%)
Suffered from anaphylactia.This is the survey data of world's metamorphosis tissue (WAO) publication, so that annual July 8 is set to the world
Anaphylactia day.
It is concentrated mainly on because of food hypersenstivity, insect allergy, plant hypersensitive, photosensitization caused by cosmetic allergic contact dermatitis, ultraviolet light
Cause skin occur itch, redness, fever, shouting pain, tissue damage symptom be application.
Causing the cause of disease of anaphylactia, there is presently no definite conclusions, but the incidence probability is caused to accelerate to increase main original
Cause is running down for living environment, and due to the quickening of process of industrialization, atmosphere pollution aggravation makes many not to be originally allergy
The people of constitution also gradually becomes allergic constitution.With the increase of vehicle, the aromatic hydrocarbon particle in automobile exhaust gas accelerates allergy again
Property inflammatory reaction occur.In addition, the chemical gas such as house decoration bring formaldehyde are also anaphylactic factor.In addition, diet
Heat and fat content increase, cause the increase of human free radical, and cause body be easy to happen allergic inflammation reaction.
Usual anaphylactogen fingering can result in the substance that immune system is abnormal reaction after entering human body, they are to cause
Quick arch-criminal.Anaphylactogen is some macromolecular substances, such as certain protein or polypeptide mostly, peanut, big such as wheat
Beans, nut, milk, egg, fish, shellfish (shrimp, crab etc.), therefore allergy has been accounted for by the anaphylactic disease that food irritability causes
90% or so of sum.In addition, there are also pollen, the inhalation (inhalatio)s such as room dirt, acarid, the microorganisms such as mould, bacterium and insect toxins
Or drug etc., these anaphylactogens may be entered in vivo by the approach such as eating, sucking, contact, inject, and cause the sensitization shape of body
When these substances are again introduced into allergic reaction can occur in vivo for state, and then excite the exception of patients immune system living
It is dynamic, ultimately cause a series of anaphylaxis injuries.
Antiallergic drug also mainly for antihistamine, experienced three stage iteration.
First generation antihistamine: such as chlorphenamine, diphenhydramine, there are apparent sedation and nervous centralis bad anti-
It answers, most commonly drowsiness and general power, reaction time extension etc..Driving should be avoided after taking this kind of drug, operate precision instrument
Equal work.In addition, such drug also has anticholinergic effect, dry can be caused, eye is dry, the symptoms such as eye-blurred, constipation, also
Glaucoma can be induced, therefore, hyperplasia of prostate, glaucoma, the low person of hepatic and renal function and gerontal patient are used with caution.
Second generation antihistamine: there are commonly cetirizine, Loratadine, Mizolastine, Ebastine etc., adverse reactions
It is few, but almost without apparent cholinolytic effect and sedation.But discovery in the recent period, astemizole (Astemizole) and RMI 9918
(terfenadine) may cause rare, and serious cardiac toxic can cause fatal arrhythmia；It is closed when with same health azoles and erythromycin
With the above-mentioned adverse reaction of exacerbation.
Third generation antihistamine: such as Desloratadine, fexofenadine, adverse reaction is lighter, with erythromycin, ketoconazole
What is with that will not generate cardiac toxic, it is also possible to there are potential control unknown risks.
Also there is the antiallergic component of plant in the market, but really effective additive is relatively fewer, is concentrated mainly on ocean
Camomile, Radix Glycyrrhizae, purslane, root of kirilow rhodiola are focusing more in skin repair maintenance, the research of additive amount and the combination of best compatibility
It is less.
In recent years, cosmetics purchase crowd quicklys increase, and consumer more pays close attention to skin sensitivity and because skin sensitivity causes
Related dermal problem, which becomes the hot spot of cosmetic industry and medical industries research, lot of domestic and foreign is caused to be made up
The very big concern and investment of product company and research work.
By inquiry, the world population more than 40% indicates that they gather around and have plenty of " sensibility skin ", self think to belong to
Sensibility skin European women reaches 55%, and America women thinks (South America and North America) 50%, and African women reaches 65%.
Three doctors at French CE center are to 18780 (male-: n=10462；Female: n=8318) consumer carried out one
" self-report skin sensitivity " investigation, the allergy experience of investigation people time in the previous year.The result shows that 61% women
Male's self-report with 32% has sensibility skin of face.According to disease symptom number, researcher is by skin sensitivity point
For three kinds of Main Patterns:
Mode 1: facial telangiectasias, Blushing relevant to vascular reaction
Mode 2: the dermoreaction that certain environmental conditions are generated, such as photaesthesia；
Mode 3: contact dermatitis contacts the dermoreaction occurred after certain substance.
In cohort, women emergence pattern 1, mode 2, the probability (respectively 78%, 72% and 58%) obvious of mode 3
Higher than male (respectively 56%, 48% and 28%).
Skin allergy is skin disease common at present, has seriously affected the physical and mental health of people.In recent years, chemicals
The side effect for treating allergy is increasingly prominent, therefore the new skin care item of the prevention and treatment of skin allergy and the Chinese herbal medicine containing antiallergic are combined into
For research hotspot.
It releives composition therefore, it is necessary to research and develop a kind of antiallergic that can be applied to skin care item.
Summary of the invention
Based on this, releive composition the object of the present invention is to provide a kind of antiallergic.
Specific technical solution is as follows:
A kind of antiallergic is releived composition, including following component:
First antiallergic composition, the second antiallergic composition and antiallergic peptide combinations；
The active constituent of the first antiallergic composition includes the pure and mild hydroxy phenylpropionyl amine benzoic acid of 4- t-butylcyclohexyl；
The active constituent of the second antiallergic composition includes stephania tetrandra extract, potassium lactate, Phenoxyethanol and glycyrrhizic acid
Dipotassium；
The active constituent of the antiallergic peptide combinations includes palmityl tripeptides -8 and acetyl group tetrapeptide -15.
In wherein some embodiments, antiallergic releive composition include following parts by weight component:
First antiallergic composition 0.15-1
Second antiallergic composition 0.15-1
Antiallergic peptide combinations 0.01-0.1.
In wherein some embodiments, antiallergic releive composition include following parts by weight component:
First antiallergic composition 0.4-0.6
Second antiallergic composition 0.4-0.6
Antiallergic peptide combinations 0.04-0.1.
In wherein some embodiments, antiallergic releive composition include following parts by weight component:
First antiallergic composition 0.5
Second antiallergic composition 0.5
Antiallergic peptide combinations 0.1.
In wherein some embodiments, the pure and mild hydroxy phenylpropionyl amine benzene of 4- t-butylcyclohexyl in the first antiallergic composition
The mass ratio of formic acid is 9-11:1, and constituent mass ratio is as follows:
| Component | Content range | Remarks |
| Rilanit special -40 | 51% | |
| Trideceth -9 | 26% | |
| 1,2 pentanediols | 14.9% | |
| 4- tert. butyl cyclohexanol | 3% | |
| Propylene glycol | 2% | |
| Deionized water | 3% | |
| Hydroxy phenylpropionyl amine benzoic acid | 0.1% |
In wherein some embodiments, stephania tetrandra extract in the second antiallergic composition, potassium lactate, Phenoxyethanol and
The mass ratio of dipotassium glycyrrhizinate is 5:0.6:0.2:0.001.
| Component | Content % | Remarks |
| Water | 94.199% | |
| Stephania tetrandra extract | 5% | |
| Potassium lactate | 0.6% | |
| Phenoxyethanol | 0.2% | |
| Dipotassium glycyrrhizinate | 0.001% |
In wherein some embodiments, the matter of palmityl tripeptides -8 and acetyl group tetrapeptide -15 in the antiallergic peptide combinations
Amount is than being 1:1.
It releives application of the composition in cosmetics it is a further object of the present invention to provide above-mentioned antiallergic.
In wherein some embodiments, the cosmetics are selected from mildy wash, toner, cream, Essence, facial mask or coloured silk
Adornment.
It is a further object of the present invention to provide a kind of cosmetics, including above-mentioned antiallergic is releived composition.
Vast territory and abundant resources in China, plant resources very abundant.According to incompletely statistics, (contain for 11146 kinds of the existing medicinal plant in China
Subspecies, mutation), adhere to 385 sections separately, 2312 belong to.How so many plant filters out becomes with cosmetics antiallergic active component
The difficult point of technology.A large amount of screenings of active constituent have become academic hot spot, in active extraction process, are conducive to bioconversion
Technology propose and conversion plant effect component, the traditional Chinese medical theory of Chinese scholar preciousness with practice resource.
Motherland's medicine has discussion to skin allergy class disease very early.It is that drug enters people through a variety of ways such as drug rash
Cause the inflammatory reaction of skin, mucous membrane after body, severe one can threat to life, it is very in Chinese medicine Gu that motherland's medicine, which is referred to as " poisoning of drug ",
Record more in nationality, according to clinical symptoms, the difference at site of pathological change and age, title is different.As recorded " leaching in " Synopsis Golden Chamber "
Excessive sore, coptis powder master it ", be equivalent to the universal eczema of modern medicine." eczema of the ear " is discussed in Golden Mirror of Medicine is equivalent to ear
Portion's eczema." four change wind " is equivalent to atopic dermatitis." infantile eczema " is equivalent to infantile eczema.Nettle rash, Chinese medicine are known as " pruritus due to wind pathogen disease ",
It contacts dermatitis and is referred to as " dermatitis rhus ".
The present invention utilizes the clear target spot of Environment Science cell level allergy mechanism study, is planted using Chinese medicine quintessence of Chinese culture screening draft
Object obtains the active component of plant by high-tech, and preferably one group of skin antiallergic composition of releiving is applied in cosmetics, solves
Skin allergic symptom.
1, allergy mechanism
Sensitive is a kind of highly sensitive skin condition, vulnerable to various factors excitation and generate shouting pain, burn
The multiple-factor syndrome of the subjective symptoms such as bright, tight, itch, looking skin are normal.Reason for that is also not exclusively clear,
It is multifactor coefficient result.Can be divided into endogenous predisposing factor for example race, age, or sex, heredity, endocrine because
Plain, certain diseases etc. and extrinsic factor such as environment, chemical substance stimulation, undesirable life style and psychological factor etc..
Sensitive mutually identifies with " skin allergy ", and skin allergy is mediated by allergy, only to those sensitization
Substance generate reaction；And sensitive is more a kind of primary stimulate the reaction, stimulant lacks anisotropic, one emphasized
Nerve modulation mechanism.
Three aspects below the possible source of the mechanism that sensitive generates:
The enhancing of 1.1 Nerve conductions
The nerve modulation mechanism of sensitive may be with endothelin receptor, thermoreceptor, and nerve conduction is factor-related.
Some scholars speculate that sensitive has the nerve endings of variation, discharge more neurotransmitters, there is unique maincenter information
The effects for the treatment of process, chronic nerve ending damage or slow neurotransmitter removing, generates this reaction jointly.
The decline of 1.2 skin barrier functions
Studies have shown that the transepidermal water loss rate of sensitive individual increases, the permeable raising of its skin barrier is prompted,
It is the main reason for sensitive skin generates.Skin barrier function is bad, not only increases outer chemical substance permeability, may also be mind
The protection being subject to through tip is reduced, and is caused the signal of sensory nerve to input and is obviously increased.
The release of 1.3 sensitive skin neurotransmitters
Sensitive causes blood vessel dilatation and certain inflammatory mediator releases related with various stimulations, in relation to inflammatory mediator
Release leads to three kinds of main characterizations of skin sensitivity symptom: facial telangiectasias, photaesthesia, contact dermatitis are sensitive people
The main appeal point of group's concern.
(1) facial telangiectasias
Facial telangiectasias is commonly called as red blood trace on face, is reduced by capillary wall elasticity, and brittleness enhancing causes
The expansion of continuous vessel inhomogeneities even ruptures.Skin of face is general red, the capillary of the visible expansion of naked eyes, be often accompanied by it is red or
Aubergine plaque-like, it is dotted, the phenomenon that linear or starlike damage.
The complicated multiplicity of the reason of forming red blood trace on face can substantially be divided into primary and two kinds secondary.Primary capillary
Blood vessel dilatation has family history, belongs to caused by heredity.What sensitive skin was frequently run onto is secondary telangiectasis, Etiological
Have following several:
A) chemical factor irritation is expanded
In the pathological factors injury tissue cell such as chemistry, allergy, lysosome membrane is easily broken, and releases a variety of hydrolases
And pro-inflammatory cytokine.A series of inflammatory cases are caused to change.Hydrolysis enzyme r e lease can produce aqtocytolysis, and it is thin to damage other tissues
Born of the same parents；Histone enzyme r e lease can cause inflammatory tissue to damage, and decompose the basilar memebrane for destroying capillary, make the penetrating of capillary
Property increase；Peptide enzyme r e lease can promote the generation of bradykinin, make local vessel expansion, and cause pain；The release of penetrating silver can
The permeability of the direct capillary that increases sharply；The release of mastocytolytic factor (also known as histamine releasing factor) can promote
Mast cell discharges histamine and expands capillary by histamine, increases vasopermeability；The release of chemotactic factor (CF) can inhale
It is mobile to areas of inflammation to draw monocyte, leukocyte recruitment is caused to infiltrate.
B) physical factor irritation is expanded
Suffer wind in field work for a long time, ultraviolet light irradiation, high temperature, the stimulation of pernio makes the tolerance of capillary be more than
Normal range (NR) causes telangiectasis to rupture, and causes red complexion or aubergine.When ambient temperature significant change, pass through receipts
Contracting and expansion capillary, adjust body temperature to control blood flow, when temperature fusion, have exceeded its tolerance range, can also draw
Telangiectasis is played.
C) high altitude climacteric expansion
When body since high altitude anoxia causes blood viscosity to increase, erythrocyte number increases, and erythrocyte aggregation is reinforced, red thin
Born of the same parents' hardness, which increases deformability, to be reduced.Platelet aggregation reinforces platelet aggregation and pH value variation, influences blood viscosity and critical
Capillary radius, the formation of the aggregation of blood platelet can obviously increase micro- resistance of blood flow, or even capilary can be caused to block.
Blood viscosity increases, and critical capilary radius increases, and increases micro- resistance of blood flow, and micro- stasis of blood stream causes telangiectasis.
In addition, hormonal dependent, locally or systemically the concurrent of disease can cause telangiectasis, but the two is medicine
It is upper common.
(2) ultraviolet induced skin allergy
The comprehensive function for being mainly shown as UVA and UVB is influenced on human skin.Large dosage, which receives ultraviolet light irradiation, to be caused
Skin sunburn, tanned, the skin allergy damage such as peak skin dosage even cutaneum carcinoma.Skin erythema induced by sun exposure is that daylight causes skin
The common manifestation of photobiology damage, caused by mainly UVB, from the perspective of histology, the essence of ultraviolet erythema is one
The acute light poison inflammatory reaction of kind, wherein intradermal vascular reaction is the basis for generating erythema.Germicidal efficacy shows that ultraviolet light irradiates
There is the early stage of erythema, papillary layer of corium telangiectasis, blood cellular constituent increases, and endothelial gap is broadening, as a result leads
Vasopermeability is caused to increase, leukocytoplania, liquid exudation.Further development will appear capillary endothelium damage, blood vessel week
Cross the inflammatory reactions such as existing lymphocyte and PMN infiltration.Basal layer of epidermis may occur in which liquefactive degeneration, prickle cell simultaneously
Once part cells show was cytoplasm uniformity, and acidophilia dyeing, core shrinkage is deep to contaminate, even if " sunburn cell ", this denaturation
Cell peripheral can have a spongiosis, vacuolization, and with inflammatory cell infiltration.
The influence factor of Skin erythema induced by sun exposure mainly with the skin type of patient, the position of irradiation and the colour of skin, physiology and disease
Reason factor is related, furthermore also has a certain impact with the intensity of ultraviolet light irradiation or dosage, the external factors such as wavelength of ultraviolet light.
(3) contact dermatitis
Contact dermatitis is generally divided into two major classes, respectively allergia contact dermatitis and stimulation according to the difference of pathogenesis
Property contact dermatitis.
A) allergic contact dermatitis (ACD)
Allergia contact dermatitis is the antigentic specificity skin allergy mediated by thymus dependent lymphocyte (T lymphocyte)
Reaction.There is a series of scytitis cellular infiltration with local skin after antigenic stimulus, inflammatory mediator release is characterized, belongs to
Tardy IV allergic reaction type, essence are antigen caused delayed allergies with sensitized individuals.Allergia contact
Dermatitis can be divided into two periods of sensitization phase and stimulating phase.
The sensitization phase is haptens and immune system initial contact.The primary objective of haptens is horn cell.Lang Gehanshi
Cell (sentry of cutaneous immunisation) is then by this antigen presentation to lymphocyte, by several stages, particular for certain primary antibody
Former memory T lymphocytes occur.
Induction period contacts skin for haptens for the second time, but current allergic reaction immediately occurred, because memory T cell is fast
Speed identifies it, and this allergic reaction can produce different symptom: red dot, bruise, itch, burning sensation.
B) irritant contact dermatitis (ICD)
Irritation contacts dermatitis, criticizes tissue cell insult caused by normal skin contact stimulation.ICD metamorphosis is wide
It is general, erythema, oedema are mainly shown as in acute stage, and decortication bits and toe form cytocyst alveolation sample and become；In chronic phase master
Show as skin crack, mossization and hyperkeratinization.Generally, it is considered that as long as the stimulus intensity of contactant is more than skin threshold of tolerance
Value, it is any to fall ill per capita.
Compared with allergia contacts dermatitis, ICD lacks hapten specificity T lymphocyte.Stimulating without moral main target is
For the barrier function of cuticula.The damage of cuticula lipides barrier and horn cell bonding force are lost and because of transepidermal water
Furfur caused by lacking is related.ICD is often accompanied by the change of skin underlying biological physical index, the drop including capacitance when occurring
Low, the increase of transepidermal water loss rate, pH value increases and declines with cortical level.
2, antiallergic of the present invention composition component of releiving is preferred
Above-mentioned antiallergic releives composition using various plants effect component, by optimizing component proportion, performance plant component
Effect maximizes." monarch " is Chinese medicine effective prescription principle, is the high level overview to prescriptions.The present invention releives in exploitation
Antiallergic combines the thought for taking full advantage of " monarch ", reaches the harmonious compatibility of a functional component, so that it is whole excellent to play it
Gesture." preventing diseases instead for the treatment of diseases " is that China's health circle is abided by so far early in the strategy that prevents diseasing preservation put forward in Huangdi's Internal Classics
The earliest thought of " putting prevention first " strategy kept.This patent with " monarch " thought sensitive kinds skin protection and
In the elimination and maintenance of relevant sensitization symptom, the prominent thought based on " prevention " is reinforced skin itself barrier function, is not only wanted
The symptom having already appeared is eliminated, and to prevent the generation again of symptom, not only to prevent the generation again of symptom, and to infuse
Meaning stops and eliminates the cause for causing the symptom, and safeguard procedures are just taken before cause does not generate.This patent constructs one kind
The skin allergy symptom that customer has occurred is eliminated in antiallergic of releiving combination, is promoted skin barrier function, is promoted skin tolerance threshold,
Maintenance sensibility skin.
The present invention has selected WK2, Comthing Tetra, antiallergic peptide combinations and is sieved by macrophage nitric oxide model
The combination is selected, for the inhibitory effect of inflammation, has finally been determined that WK2, Comthing Tetra, antiallergic peptide enter further patent
Plan-validation.
2.1 first antiallergic compositions (WK2 (moral fragrant))
First antiallergic composition uses the fragrant WK2 raw material of moral, and active component is the pure and mild hydroxyphenyl of 4- t-butylcyclohexyl
Propionamide benzoic acid, wherein 4- tert. butyl cyclohexanol act on cell-cell communication it is horizontal-directly with specific neuroceptor,
TRPV1 thermoreceptor is in contact, and intercept information prevents from being in contact with its neuroceptor, enhances the tolerance threshold value of skin.Make
For sensitive knob, the flow of calcium in cell membrane can be reduced, the sensibility of skin is made to be restored to normal level, utilizes hydroxyphenyl third
Acid amides benzoic acid, antagonizing histamine, antipruritic inhibition oedema.Its component see the table below:
2.2 second antiallergic compositions (Comthing Tetra)
Second antiallergic composition uses Comthing Tetra, and active constituent includes stephania tetrandra extract, potassium lactate, benzene oxygen
Ethyl alcohol and dipotassium glycyrrhizinate have and rebuild skin barrier, protect inflammation caused by bacterium, and component is as follows:
| Component | Content % | Effect | Remarks |
| Stephania tetrandra extract | 0.3 | Inhibit inflammation, oedema | |
| Potassium lactate | 0.65 | Repair skin barrier | |
| Dipotassium glycyrrhizinate | 0.2 | Inhibit histamine release, inhibits inflammation | |
| Phenoxyethanol | 0.5 | Antibacterial components | |
| Water | To 100 | Dissolution |
Second antiallergic composition is using antiallergic component stephania tetrandra extract main in Comthing Tetra and glycyrrhizic acid two
The organic assembling of potassium divides stephania tetrandra extract effect group to be divided into tetrandrine, is able to suppress the lymphocyte of division primary stimuli
Proliferation promotes cellular immunity, inhibits the generation of oxygen radical, inhibits oil peroxidation reaction, eliminates seborrhea, inhibits
Arachidonic acid and the oedema for intersecting glycosides induction, can almost completely inhibit the activity of hyaluronidase；Dipotassium glycyrrhizinate can
The threshing effect for stablizing mast cell, inhibits the release of histamine, makees to direct cutaneous contact dermatitis with good inhibition
With, excellent antiallergic effect, calm condition susceptible.
2.3 antiallergic peptide combinations
Allergy belongs to inflammatory reaction, refers to that, when some exotics invade human body, the immune system of human body generates excessive
Reaction.Specifically, allergy is the allergy that exotic antigen is read as to harmful object and is generated, and releases inflammatory mediator
(such as histamine, prostaglandin), Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 and inflammatory cytokine such as (1L-8), cause red, the symptoms such as swollen, heat, pain, serious to peel,
Oedema.
Antiallergic peptide combinations contain -15 two kinds of high activity polypeptides of peptide palmityl tripeptides -8 and antiallergic peptide acetyl group tetrapeptide of releiving
The high-end compound material of ingredient.The inflammatory reaction that they are directed to the overreaction and skin allergy of body respectively is worked, and is improved
The tolerance of body reduces inflammatory reaction, can play prevention and allergic symptom of releiving.
(1) it releives peptide
The entitled palmityl tripeptides -8 of the peptide INCI that releives, the anti-inflammatory activity with high special, and potentially reduce black
The generation of element, peptide of releiving is lipopeptid, is made of three amino acid, and is coupled with palmitinic acid.Rouge coupling be it is known, can promote peptide
It is absorbed by skin.
Peptide of releiving, which mitigates, stimulates caused consequence by various factors, reduces Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 and discharges some column symptoms caused；Suppression
Inflammatory reaction processed reduces the release of proinflammatory factor, including 1L-1,1L-8,Reduce blood vessel extension and permeability.Effectively
Inhibit the proinflammatory factor 1L-8 of UVB induction to discharge EXVIVO, the prior step EX VIVO of cascade of response of inflammation, energy can be inhibited
Effectively prevent and mitigate the inflammation that chemical stimulation causes.
(2) antiallergic peptide
Human skin contains there are many Sensory nerve fibre, until epidermis, these nerve fibres can by central nervous system
To be aware of the situation of skin at any time, and to various chemical reactions, heat and physiological stimulation are made a response.
Sensitive skin is generally considered skin barrier function and is damaged, and transepidermal water, which scatters and disappears, to be increased, the transdermal suction of chemical substance
Receipts enhancing, easily infiltrates into environmental stimuli object, leads to the incoming enhancing of dermal sensation Holy Bible signal, and tolerance threshold is reduced down to micro-
The overreaction of weak stimulant then causes all kinds of skin problems such as itch, and shouting pain is red and swollen, and all kinds of skin such as tight are uncomfortable
Symptom.
The neural source height reaction of sensitive skin is usually related with the release action of TRPV1 and neuropeptide, such as reduces calcium element phase
It closes peptide (CGPR).
The INCI title of antiallergic peptide are as follows: acetyl group tetrapeptide -15, a kind of polypeptide that can improve skin natural tolerance threshold value lead to
It crosses and improves skin to the tolerance of environmental factor, link reaches link since cosmetics and skin treating means bring are uncomfortable
The deeply grateful purpose of skin, this polypeptide can reduce the natural opioid peptides of the stimulation of cutaneous nerve tip similar to one kind.
Antiallergic peptide can be reduced the quantity of the pro-inflammatory mediator calcitonin gene-related peptide (CGRP) of human body generation, and it is right to reduce nerve
The tetchiness of environmental stimuli.Experiment in vitro proves that antiallergic peptide acts on opiate receptor, has high selectivity to receptor,
By in conjunction with receptor, inhibiting neurotransmitter (CGRP) release, the stimulation and excitement of neuron are reduced, the threshold of tolerance of skin is improved
Value makes skin to various stimulations, the discomfort decreased sensation such as pain.
Antiallergic of the present invention releive composition using peptide of releiving with high specific anti-inflammatory activity, reduce inflammatory reaction, prevention
With alleviation malaise symptoms；The reaction of cutaneous nerve source property height is reduced by antiallergic peptide, improves tolerance of skin.
Above-mentioned antiallergic composition of releiving has the advantages that
1) above-mentioned antiallergic releives composition by WK2 and Comthing tetra, the exploitation combination of antiallergic peptide combinations, by powder
The pure and mild hydroxy phenylpropionyl amine benzoic acid of Radix Stephaniae Tetrandrae extract, dipotassium glycyrrhizinate, 4- t-butylcyclohexyl, peptide of releiving, six kinds of antiallergic peptide
The combination of antiallergic effect, endogenous upper inhibition mast cell histamine release inhibit arachidonic acid to decompose Prostaglandin PGE2 and white three
Alkene inflammatory factor, inhibits keratinocyte, and mast cell discharges interleukin-11 L-6 and anti-tumor necrosis factorTo
Alleviate rubescent caused by skin allergy, fever, itch, tight, edematous condition；Nitric oxide inhibiting rate is 38.62%；Histamine suppression
Rate processed reaches 41.92%；
2) organic assembling of main antiallergic component stephania tetrandra extract and dipotassium glycyrrhizinate in Comthing Tetra is used,
It divides stephania tetrandra extract effect group to be divided into tetrandrine, is able to suppress the lymphopoiesis of division primary stimuli, promotes cell
Immunity, inhibits the generation of oxygen radical, inhibits oil peroxidation reaction, eliminates seborrhea, inhibit arachidonic acid and
Intersect the oedema that glycosides induces, can almost completely inhibit the activity of hyaluronidase；Dipotassium glycyrrhizinate can stablize mast cell
Threshing effect, inhibit the release of histamine, to direct cutaneous contact dermatitis have good inhibiting effect, excellent antiallergic
Effect, calm condition susceptible.
3) the antiallergic peptide combinations used contain -15 two kinds of height of peptide palmityl tripeptides -8 and antiallergic peptide acetyl group tetrapeptide of releiving
The high-end compound material of active peptides ingredient.Peptide of releiving, which mitigates, stimulates caused consequence by various factors, reduces Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 and discharges institute
The some column symptoms caused；Inhibit inflammatory reaction, reduce the release of proinflammatory factor, including 1L-1,1L-8,Reduce blood
Pipe extension and permeability；Antiallergic peptide can be reduced the quantity of the pro-inflammatory mediator calcitonin gene-related peptide (CGRP) of human body generation, drop
Tetchiness of the low nerve to environmental stimuli.Experiment in vitro proves that antiallergic peptide acts on opiate receptor, has height to receptor
Selectivity reduces the stimulation and excitement of neuron, improves skin by conjunction with receptor, inhibiting neurotransmitter (CGRP) release
The tolerance threshold of skin makes skin to various stimulations, the discomfort decreased sensation such as pain.
4) by WK2 and Comthing tetra, the exploitation combination of antiallergic peptide combinations, by stephania tetrandra extract, glycyrrhizic acid
Six kinds of antiallergics such as the pure and mild hydroxy phenylpropionyl amine benzoic acid of dipotassium, 4- t-butylcyclohexyl, palmityl tripeptides -8, acetyl group tetrapeptide -15
Effect combination, endogenous upper inhibition mast cell histamine release inhibit arachidonic acid to decompose Prostaglandin PGE2 and leukotriene inflammation
Inflammation factor, inhibits keratinocyte, and mast cell discharges interleukin-11 L-6 and anti-tumor necrosis factorPass through palm
The inflammatory reaction of acyl tripeptides -8 and acetyl group tetrapeptide -15 respectively for the overreaction and skin allergy of body is worked, and is improved
The tolerance of body reduces inflammatory reaction, can play prevention and allergic symptom of releiving, thus hair caused by alleviating skin allergy
It is red, fever, itch, tight, edematous condition.
Detailed description of the invention
Fig. 1 is histamine canonical plotting；
Fig. 2 is the standard solution chromatogram of histamine；
Fig. 3 is nitric oxide releasing amount inhibitory effect figure；
Fig. 4 is histamine release amount inhibitory effect figure.
Specific embodiment
It to facilitate the understanding of the present invention, below will be to invention is more fully described.But the present invention can be to be permitted
Mostly different form is realized, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes
It is more thorough and comprehensive to the understanding of the disclosure.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
1, component screening
To antiallergic component WK2 (the first antiallergic composition), Comthing Tetra (the second antiallergic composition) and antiallergic peptide
Composition is configured to lotion, carries out efficacy assessments.
Table 1
2, formulation ratio
In order to more preferably releive component efficacy assessments to antiallergic, antiallergic component application is prepared into anti-allergic emulsion, formula and
Technique is as follows:
3, Recipe
Contrast groups 1-6 and embodiment 1-3 are separately added into formula, antiallergic lotion of releiving is prepared into and carries out effect evaluation and test.
Antiallergic releive lotion preparing process it is as follows:
1) glycerol, propylene glycol, butanediol, the glycine betaine of corresponding percentage are weighed according to formulation ratio, allantoin, shell are few
Sugar, compound amino acid, oligomerization Sodium Hyaluronate, pyrrolidone sodium carboxylate；
2) card wave U20, arginine are weighed according to the proportion；
3) deionized water of corresponding proportion is added, heats up as 80-85 DEG C, stirs 250-300rpm, stir 30min.
4) it is added sad certain herbaceous plants with big flowers acid glycerol three ester, Dow corning, temperature is controlled at 80-85 DEG C, with 6500-7500rpm,
Homogeneous 3min.
5) temperature reduces 55-60 DEG C, and ceramide liposome, ultimate attainment reparation peptide is added, and stirring stirring 180-250rpm is stirred
Mix 30min.
6) 45 degrees Celsius are cooled to, control sample component (or embodiment sample component), parahydroxyacet-ophenone and Phenoxyethanol stir
80-100rpm is mixed, 10min is stirred, is cooled to room temperature.
4, effect is evaluated and tested
4.1LPS induces RAW264.7 cell to discharge NO inhibiting effect
4.1.1 experimental principle and method
LPS (lipopolysaccharides):
Positioned at the lipopolysaccharides substance of gram-negative bacterial cell wall outermost one layer thicker (8-10nm), by class
Rouge A, core polysaccharide and 3 part of O- specific side chain composition.Lipopolysaccharides is endotoxin and important group specific antigen (O antigen).Rouge
Polysaccharide consists of three parts.Lipid A (Lipid A) is the glycolipid for constituting activity of endotoxin, with covalent bonding junction to heteroglycan chain,
There are two parts: first is that core polysaccharide, is constant in related strain；Another O specificity chain (O-specific chain) is
Alterable height.The lipopolysaccharides of Escherichia coli is the i.e. polyclonal work of common B cell mitogeneic factor in Experimental immunization
Change the factor (polyclonal activator).This experiment generates allergic reaction using LPS induction RAW264.7 cell, discharges NO
(nitric oxide).
Griess assay-NO content test method:
NO is easily oxidized to NO in vivo or in aqueous solution2, in acid condition, NO and diazonium salt sulfanilamide (SN) generation diazonium are anti-
It answers, and generates diazonium compound, coupling reaction further occurs with naphthylethenyl diamines for the latter, and the product which generates is dense
Degree has linear relationship with NO concentration, there is maximum absorption band at 540nm.
Experimental material and method:
Material: DMEM, FBS, LPS, MTT, Griess reagent
Experimental method:
264.7 cell culture of RAW
RAW264.7 cell is drawn into DMEM training base with liquid-transfering gun and dispels cell dispersion, is counted using Hemacytometer
Number cell, then using DMEM come diluting cells, being diluted to concentration is 5 × 104cells/ml.
Cell solution after dilution is inoculated into 96well respectively, and each hole is 100ul, i.e. 5 × 103cells/well.
It is cultivated 24 hours in 37 DEG C, the incubator of 5%CO2.
Sample to be tested and blank sample prepare: sample to be tested is trained base with DMEM and is diluted, and the concentration after dilution is respectively as follows: 1% (should
Concentration is tested by the MTT of front, and result is nontoxic)
Sample composition LPS 10ppm LPS 10ppm+1%sample
After cell culture 24 hours, the whether complete adherent growth of cell is observed, it, will if the complete adherent growth of cell
Original training base removes, and is washed with DPBS.
After DPBS is removed, it is separately added into the ready sample in front.The solubility of sample is according to the result of toxotest
It selects safe concentration 1% (20ul/well).
After sample is added, 37 DEG C are put into, is cultivated 20 hours in the incubator of 5%CO2.
It after 20 hours, pipettes on the culture medium to new 96Well of 50ul, the 1%Griess of same volume is added
Reagent after being sufficiently mixed, reacts 10 minutes in dark place.
Utilize the absorption photometric value of ELISA reader test sample at 540nm.
NO inhibiting effect:
NO Inhibition%=((" OD " _ Control- " OD " _ Sample)/" OD " _ Control) * 100%
Wherein: the absorption photometric value of " OD " _ Control- blank sample
The absorption photometric value of " OD " _ Sample- sample
4.1.2 component efficacy assessments
It control sample or embodiment sample is added to antiallergic according to the proportion of table 1 releives and utilize macrophage nitric oxide in lotion
Inflammatory model is inhibited to carry out efficacy assessments (as shown in Table 2 and Fig. 3).
2 antiallergic of table is releived the NO burst size inhibitory effect result of composition
| NO Release Decrease (%) | Standard deviation | |
| Contrast groups 1 | 31.26 | 5.86 |
| Contrast groups 2 | 22.37 | 3.83 |
| Contrast groups 3 | 19.32 | 1.82 |
| Contrast groups 4 | 36.27 | 4.29 |
| Contrast groups 5 | 33.67 | 2.75 |
| Contrast groups 6 | 19.66 | 1.41 |
| Embodiment 1 | 38.62 | 1.61 |
| Embodiment 2 | 39.23 | 4.42 |
| Embodiment 3 | 37.42 | 3.58 |
The inhibiting effect of 4.2 induction P815 mouse macrophage oncocyte histamine release amounts
4.2.1 experimental principle:
1) the HPLC detection method of histamine content
Histamine is both without fluorescence chromophoric group or without ultraviolet chromophoric group, but dansyl Cl can be the same as the primary amine of histamine
Or the active hydrogen reaction in secondary amine, the HCl for taking off an one's share of expenses for a joint undertaking generate the derivative with fluorescence and ultraviolet light, reaction equation is such as
Under:
2) experimental material and method:
Material: DMEM, FBS, MTT, dansyl Cl, NaHCO3, ammonium hydroxide, acetonitrile, ammonium acetate
Experimental method:
P815 cell culture:
(1) DMEM training base is drawn with liquid-transfering gun and dispel P815 cell cell dispersion, counted using Hemacytometer thin
Born of the same parents, then using DMEM come diluting cells, being diluted to concentration is 5 × 104cells/ml。
(2) cell solution after releasing is inoculated into respectively in 6well petri dish, each hole be 1ml, i.e., 0.5 ×
107cells/well。
It (3) 37 DEG C, is cultivated 24 hours in the incubator of 5%CO2.
(4) sample to be tested and blank sample prepare: sample to be tested is trained base with DMEM and is diluted, and the concentration after dilution is respectively as follows: 2%
(concentration is tested by the MTT of front, and result is nontoxic).
| Title | Blank sample | To test sample |
| Sample composition | UV irradiation | UV irradiation+2%sample |
After (5) 24 hours, 6well petri dish is placed in irradiation 20 seconds below 15W ultraviolet lamp, induction P815 is thin
Born of the same parents generate allergic reaction, discharge histamine.
(6) since P815 cell is the growth of half wall-attaching type, add 1ml's under the premise of being changed without Pei Ji, in blank sample
DMEM trains base, and the culture medium for containing 2% sample to add 1ml in test sample makes the concentration of sample in total culture medium reach 1%.
(7) after sample is added, 37 DEG C is put into, is cultivated 24 hours in the incubator of 5%CO2.
After (8) 24 hours, the culture medium for pipetting 200ul carries out the derivative reaction of histamine.
3) the HPLC analysis of histamine
(1) analyte derivative
It takes 100ul culture based in 2ml tube, sequentially adds and be saturated NaHCO3 solution 200ul, 10mg/ml dansyl Cl
Solution 400ul, lid plug whirlpool after mixing, are protected from light 60 minutes at room temperature.20% ammonium hydroxide-is added in reaction after sixty minutes
Acetonitrile solution 100ul, the reaction of termination in static 30 minutes.Whirlpool is uniformly mixed after 100ul acetonitrile is added, and is crossed 0.45um filter membrane and is carried out
HPLC analysis.
(2) standard working solution is derivative
It takes suitable histamine normal storage liquid to be made into 1,5,10,50,100ppm standard working solution respectively, takes 100ul
In 2ml tube, the installation above method performs the derivatization reaction.After the filtering of 0.45um film, HPLC analysis is carried out.
(3) chromatographic condition
Chromatographic column: Agilent C18 reverse-phase chromatographic column, 250mm × 4.6mm, 5um
Mobile phase: acetonitrile: 0.1mol/L ammonium acetate=80:20 (v:v)
Flow velocity: 1ml/min
Column temperature: 35 DEG C
Sample volume: 20ul
Ultraviolet detection wavelength: 254nm
4) experimental result and analysis
(1) standard curve
Under the chromatographic condition that the experiment is established, with histamine chromatographic peak area (Y) for ordinate, with corresponding concentration (X)
For abscissa, draw standard curve (as shown in Figure 1).
The peak area of the histamine titer of various concentration:
| Concentration (ppm) | Peak area |
| 1 | 336.5 |
| 5 | 1455.8 |
| 10 | 3071.4 |
| 50 | 13530 |
| 100 | 26963.6 |
For histamine standard working solution in the concentration range of 1-100ppm, peak area and concentration are in good linear relationship,
Equation of linear regression is y=267.8x+180.58, R2=0.9999.The standard solution chromatogram of histamine is as shown in Figure 2.
4.2.2 effect evaluation result (as shown in table 3 and fig. 4)
3 antiallergic of table is releived the histamine release amount inhibitory effect result of combination of compositions
Conclusion: antiallergic of the present invention releives composition by WK2 and Comthing tetra, antiallergic peptide combinations development group
It closes, by stephania tetrandra extract, dipotassium glycyrrhizinate, the pure and mild hydroxy phenylpropionyl amine benzoic acid of 4- t-butylcyclohexyl, peptide of releiving, antiallergic peptide
Six kinds of antiallergic effects combination, endogenous upper inhibitions mast cell histamine release, inhibit arachidonic acid decomposition Prostaglandin PGE2
With leukotriene inflammatory factor, inhibit keratinocyte, mast cell discharges interleukin-11 L-6 and anti-tumor necrosis factor TNF-
A, to alleviate rubescent caused by skin allergy, fever, itch, tight, edematous condition；Nitric oxide inhibiting rate is 38.62%；
Histamine inhibiting rate reaches 41.92%.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (9)
- A kind of composition 1. antiallergic is releived, which is characterized in that the component including following parts by weight:First 0.15-1 parts of antiallergic composition, 0.15-1 parts of the second antiallergic composition and 0.01-0.1 parts of antiallergic peptide combinations；The active constituent of the first antiallergic composition includes the pure and mild hydroxy phenylpropionyl amine benzoic acid of 4- t-butylcyclohexyl；The active constituent of the second antiallergic composition includes stephania tetrandra extract, potassium lactate, Phenoxyethanol and dipotassium glycyrrhizinate；The active constituent of the antiallergic peptide combinations includes palmityl tripeptides -8 and acetyl group tetrapeptide -15.
- The composition 2. antiallergic according to claim 1 is releived, which is characterized in that the component including following parts by weight:First antiallergic composition 0.4-0.6Second antiallergic composition 0.4-0.6Antiallergic peptide combinations 0.04-0.1.
- The composition 3. antiallergic according to claim 1 is releived, which is characterized in that the component including following parts by weight:First antiallergic composition 0.5Second antiallergic composition 0.5Antiallergic peptide combinations 0.1.
- The composition 4. antiallergic according to claim 1-3 is releived, which is characterized in that the first antiallergic composition The mass ratio of the middle pure and mild hydroxy phenylpropionyl amine benzoic acid of 4- t-butylcyclohexyl is 9-11:1.
- The composition 5. antiallergic according to claim 1-3 is releived, which is characterized in that the second antiallergic composition Middle stephania tetrandra extract, potassium lactate, Phenoxyethanol and dipotassium glycyrrhizinate mass ratio 5:0.6:0.2:0.001.
- The composition 6. antiallergic according to claim 1-3 is releived, which is characterized in that in the antiallergic peptide combinations The mass ratio of palmityl tripeptides -8 and acetyl group tetrapeptide -15 is 1:1.
- 7. antiallergic described in any one of claims 1-6 is releived, composition is preparing the application in cosmetics.
- 8. application according to claim 7, which is characterized in that the cosmetics are selected from mildy wash, toner, cream, essence Magnificent liquid, facial mask or color make-up.
- 9. a kind of cosmetics, which is characterized in that releive composition including antiallergic described in any one of claims 1-6.
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| CN109172586B (en) * | 2018-07-06 | 2021-01-19 | 福州百草堂医药科技有限公司 | A composition for relieving skin irritation |
| CN109010113B (en) * | 2018-08-28 | 2021-08-17 | 高宝化妆品（中国）有限公司 | Composition for repairing sensitive skin and application thereof |
| CN109125107B (en) * | 2018-09-20 | 2022-02-01 | 深圳市维琪医药研发有限公司 | Polypeptide composition for effectively improving and repairing facial hormone-dependent dermatitis |
| CN109172397A (en) * | 2018-09-20 | 2019-01-11 | 深圳市鑫煌倚天科技有限公司 | A kind of cosmetics |
| CN109010115A (en) * | 2018-10-19 | 2018-12-18 | 中山爱护日用品有限公司 | A kind of Shu Min creams with anti-inflammatory efficacy |
| CN109464373A (en) * | 2018-11-20 | 2019-03-15 | 上海悦目化妆品有限公司 | A kind of antiallergic facial treatment mask |
| CN110075059B (en) * | 2019-05-29 | 2021-07-06 | 广州睿晞生化科技有限公司 | Composition capable of resisting allergy, relieving itching and repairing |
| CN111632001A (en) * | 2020-07-02 | 2020-09-08 | 广州美兮生物科技有限公司 | Multifunctional toning lotion and preparation method thereof |
| CN112137931B (en) * | 2020-10-21 | 2023-09-22 | 上海科颜生物科技有限公司 | Composition for relieving sensitive skin and application thereof |
| CN114392226B (en) * | 2022-02-24 | 2023-09-26 | 武汉百思凯瑞生物科技有限公司 | Anti-allergic polypeptide nano composition and preparation method and application thereof |
| CN115040467B (en) * | 2022-07-29 | 2024-05-03 | 哈尔滨敷尔佳科技股份有限公司 | Composition for relieving and resisting allergy, preparation method and application thereof |
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