CN109288884B

CN109288884B - Preparation method and application of sunflower extract with soothing and repairing effects

Info

Publication number: CN109288884B
Application number: CN201811541249.8A
Authority: CN
Inventors: 刘明富
Original assignee: Guangzhou Shimei Cosmetics Co ltd
Current assignee: Guangzhou Shimei Cosmetics Co ltd
Priority date: 2018-12-17
Filing date: 2018-12-17
Publication date: 2021-09-07
Anticipated expiration: 2038-12-17
Also published as: CN109288884A

Abstract

The invention discloses a preparation method and application of a sunflower extract with a soothing and repairing effect. The method comprises the following steps: (1) mixing sunflower discs and sunflower seed cakes according to a weight ratio of 1: 0.01-1, and crushing to obtain sunflower powder; (2) adding a solvent into the sunflower powder, and extracting at the temperature of 60-80 ℃ to obtain an extracting solution; then centrifuging the extracting solution, and collecting filtrate; and concentrating the filtrate after membrane separation to obtain the sunflower extract. The sunflower disc and the sunflower seed cake are reasonably compounded, and then the pure natural extract with the effect of relieving and repairing is prepared through extraction and separation. Therefore, the sunflower extract can be applied to cosmetics or medicines with the functions of relieving allergy and repairing damaged skin.

Description

Preparation method and application of sunflower extract with soothing and repairing effects

Technical Field

The invention belongs to the field of natural medicinal chemistry and the technical field of cosmetics, and particularly relates to a preparation method and application of a sunflower extract with a soothing and repairing effect.

Background

Sunflower (Helianthus annuus L.) is one of the important oil crops in China and the earliest edible crops in China, has a history of 5000-6000 years to date, and is widely applied to the fields of pharmaceutical chemicals, food and the like. The oil production is inferior to soybean, the cultivation area is inferior to soybean and rape, the third place is that the sunflower seeds as fruit can be eaten for a long time besides being used for oil pressing, and the sunflower seeds have very good health care function. At present, the extraction of sunflower oil is already realized in industrial production, but byproducts such as sunflower discs, sunflower seed shells, cake and the like are still not utilized sufficiently.

Regarding the utilization of sunflower-related by-products, the main focus is on flower discs, and other reports are less. For example, the biological technology 201020 (2)74-77 of the shinyleaf solomonseal rhizome and the like extracts water-soluble polysaccharide from sunflower discs, and the Chinese patent CN106386873A of the shinyleaf azolla herb and the like uses the sunflower discs as raw materials and obtains the plant-derived bacteriostatic agent for inhibiting the fusarium oxysporum by a water extraction centrifugation method. Guochet et al (Chinese patent CN 108379318A) uses sunflower discs as raw material, and extracts are obtained by centrifuging with water to obtain precipitate, and ultrafiltering and filtering with 0.45um filter membrane to obtain extract for inhibiting xanthine oxidase activity. Jieyong et al (Chinese patent CN 107049883A) use sunflower seed oil to extract oil soluble substances from sunflower seed cake, and the obtained extract has sunscreen and synergistic effects.

Skin allergies are also known as "sensitive" skin. From the medical point of view, skin allergy mainly refers to the abnormal phenomena of skin inflammation, itching and pain, desquamation, allergic dermatitis and the like caused by various stimulations to the skin. Under the derivation of green, environment-friendly, chongshang nature and returning nature concepts, natural plants are more and more widely applied in the field of cosmetics. Researches show that leaves, flowers, flower discs, shells, roots, stem pith and the like of the sunflower can be used as medicines, and have good medicinal value. The main chemical components of the composition comprise terpenoids such as sesquiterpene, diterpene, sesquiterpene lactone, triterpenes, etc., and also comprise sterol, coumarin, phenols, flavonoids, volatile substances, etc. Wherein, the sunflower discs contain chlorogenic acid, terpenes, flavonoids and other active substances which not only have bacteriostatic effect, but also have antioxidant activity. Sunflower seeds contain 21-30% of protein, 28-32% of grease and abundant VE and VB₆And carotene, and further contains 12.6% of saccharides, 2.7% of cellulose, and 4.4% of ash, and has high contents of trace elements and various vitamins, and a large amount of effective components remain in the cake after the oil and fat are extracted. Thus, it is possible to provideThe effective components of the sunflower discs and the cakes are fully utilized, and the method has very important significance for further improving the corresponding economic value in the industrial chain. However, no reports related to the anti-allergy aspect after the two are compounded are found at present.

Therefore, it is very necessary to invent a sunflower extract with a soothing and repairing effect.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provide a preparation method of a sunflower extract with a soothing and repairing effect.

The invention also aims to provide the sunflower extract with the effect of relieving and repairing, which is prepared by the method.

Still another object of the present invention is to provide the use of sunflower extract with soothing and repairing action.

The purpose of the invention is realized by the following technical scheme: a preparation method and application of sunflower extract with soothing and repairing effects comprise the following steps:

(1) mixing sunflower discs and sunflower seed cakes according to a weight ratio of 1: 0.01-1, and crushing to obtain sunflower powder;

(2) adding a solvent into the sunflower powder obtained in the step (1), and extracting at the temperature of 60-80 ℃ to obtain an extracting solution; then centrifuging the extracting solution, and collecting filtrate; and concentrating the filtrate after membrane separation to obtain sunflower extract.

The weight ratio of the sunflower discs to the sunflower seed cakes in the step (1) is preferably 1: 0.05 to 1.

The particle size of the sunflower powder in the step (1) is 10-100 meshes; preferably 20 to 80 mesh.

The weight ratio of the sunflower powder to the solvent in the step (2) is 1: 1-30; preferably 1: 5-20.

The solvent in the step (2) is one or a mixture of more than two of water, an alcohol solvent or an ester solvent; preferably one or a mixture of more than two of water, ethanol, ethyl acetate and butanediol; more preferably 50-95% (w/w) ethanol aqueous solution, ethyl acetate, or 50% (w/w) butanediol aqueous solution.

The extraction time in the step (2) is 30-180 minutes; preferably 60 to 180 minutes.

The membrane separation described in step (2) is preferably achieved by: and separating the filtrate by using a ceramic membrane with the aperture of 500 nm-1000 nm and an ultrafiltration membrane with the aperture of 3000 Da-10000 Da in sequence.

The concentration in the step (2) is carried out at 40-80 ℃ and-0.1-0.3 MPa vacuum degree; preferably, the concentration is carried out at 60-80 ℃ and-0.1-0.2 MPa of vacuum degree until the relative density measured at 25 ℃ is 1.05-1.15 (preferably 1.07-1.11).

A sunflower extract prepared by any one of the above methods.

The sunflower extract is applied to the field of cosmetics.

The cosmetics are preferably cosmetics with functions of relieving allergy and repairing damaged skin, and comprise cream emulsions, film powders, jelly, aerosols, pressed powders and the like.

The addition amount of the sunflower extract accounts for 1-50% of the weight of the cosmetic; preferably accounts for 1-5% of the weight of the cosmetic; more preferably 5% by weight of the cosmetic.

The cosmetic also includes a cosmetically acceptable base form.

A soothing and repairing skin cream comprises the following components in percentage by weight:

phase A: 10% of mineral oil; 2% of cocoa butter; vitamin E (vitamin E) 1.3%; 6% of emulsifying wax; 2.8% of glyceryl monostearate; 4% of octadecanol;

phase B: 1-5% of the sunflower extract;

and C phase: 2% of glycerol; 2% of propylene glycol; 0.5 percent of acetic acid monoethanolamide; 0.2 percent of triethanolamine; the deionized water is added to make up to 100 percent.

The emulsifying wax in the phase A is preferably GP2000 emulsifying wax.

The addition amount of the sunflower extract in the B phase is preferably 5% of the weight of the soothing and repairing skin cream.

The phase C also contains a preservative; IS-45 preservative IS preferred.

The addition amount of the preservative is preferably 0.45 percent of the weight of the soothing and repairing skin cream.

The preparation method of the soothing and repairing skin cream comprises the following steps:

(1) mixing the components in the phase A, heating to 70 ℃ to completely melt the components to obtain a phase A mixture;

(2) mixing the components in the phase C, heating to 70 ℃, and uniformly stirring to obtain a phase C mixture;

(3) and adding the phase A mixture into the phase C mixture for emulsification reaction, cooling to 40 ℃ after emulsification is finished, adding the components in the phase B, uniformly stirring, and cooling to room temperature to obtain the soothing and repairing skin cream.

The sunflower extract is applied to the preparation of medicaments for inhibiting hyaluronidase, diminishing inflammation, inhibiting bacteria, improving allergic constitution or resisting allergy.

The allergy includes allergy caused by capsaicin or lactic acid.

The sunflower extract is applied to the preparation of a hyaluronidase inhibitor.

The sunflower extract is used as a hyaluronidase inhibitor.

According to the invention, sunflower resources are fully utilized, namely, effective components of sunflower discs and cakes are utilized, wherein the sunflower discs contain chlorogenic acid, terpenes, flavonoid compounds and other active substances, and the substances not only have an antibacterial effect, but also have antioxidant activity; sunflower seeds contain 21-30% of protein, 28-32% of grease and abundant VE and VB₆And carotene, etc. and also contains saccharide 12.6%, cellulose 2.7% and ash 4.4%, and has high content of trace elements and vitamins, so that even the cake after squeezing oil and fat still has a large amount of effective components, sunflower seed protein and chlorogenic acid can be extracted from the cake.

Skin allergy of human body mainly refers to skin diseases such as red and swollen skin, itching, wheal, desquamation and the like caused by contacting human body with certain allergens. Skin disorders caused by allergy include mainly contact dermatitis and cosmetic dermatitis. Contact dermatitis refers to the condition of erythema, edema, itching and pain occurring locally after the skin is contacted with a certain substance, and in severe cases, the skin may have the symptoms of blister, desquamation, etc. Substances capable of causing skin to generate contact dermatitis include jewelry, watch chains, spectacle frames, sandals, chemical fiber cloth, external medicines, chemicals, cosmetics and the like. When the symptoms are found, people who are easy to generate contact dermatitis belong to allergic people in many cases. Cosmetic allergy cosmetic can cause allergic dermatitis symptoms such as red, swollen, hot, painful, blister, etc. Medically, it is best not to use it if it is found to be allergic to certain cosmetic products. But sometimes the hair is not separated in life, such as hair dyeing for middle-aged and old people and light make-up for professional women. The most scientific approach is to improve the allergic constitution fundamentally. Therefore, the mainstream antiallergic method at present is to eliminate or relieve skin inflammation, improve the defense function of the organism and improve the environment and keep away from the allergic source. The sunflower extract disclosed by the invention can effectively repair skin barriers, relieve body damage caused by allergic inflammation and improve the immunity of a body.

Compared with the prior art, the invention has the following advantages and effects:

1. the invention prepares the pure natural extract with the effect of relieving and repairing through extraction and separation after reasonably compounding the sunflower discs and the sunflower seed cakes. The invention utilizes the active substances such as chlorogenic acid, flavonoid and the like with the functions of diminishing inflammation and inhibiting bacteria in the flower disc of the sunflower to play a role in inhibiting the release of inflammatory reaction factors causing allergy; the components such as VE, carotene, polysaccharide and the like with repairing effect in the sunflower seed cake are utilized to relieve and repair the body damage caused by allergic inflammation and improve the immunity of the body.

2. The efficacy experiment proves that the extract has obvious effect of inhibiting hyaluronidase, and the inhibition rate of the extract is increased along with the increase of the application concentration; simultaneously, the stabbing pain caused by the capsaicin and the lactic acid can be relieved; the human body uses the repair skin cream of the invention for 28 days to show that the extract of the invention can obviously repair the barrier function of the damaged skin.

3. The extraction method provided by the invention is simple to operate and short in production period, and the obtained extract can be applied to cosmetics with the functions of relieving allergy and repairing damaged skin (the addition amount is 1% -50%), and has important significance for further improving the corresponding economic value in the industrial chain.

Drawings

FIG. 1 is a graph showing the hyaluronidase inhibition effect of the sunflower extracts of examples 1-4 at different application concentrations.

FIG. 2 is a graph showing the effect of the sunflower extract of examples 1 to 4 in significantly increasing hyaluronidase inhibition with increasing application concentration.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise specified; unless otherwise specified, reagents and starting materials for use in the present invention are commercially available.

Example 1

Mixing 1kg commercially available sunflower discs (commercially available sunflower discs are products which are removed of fruits and dried in the sun) and 0.1kg sunflower seed cakes (Shandong Qiaowie food science and technology Co., Ltd., the same below) uniformly, pulverizing into 40 mesh powder, adding 25kg of 50% (w/w) ethanol aqueous solution, extracting at 60 deg.C for 120 min, centrifuging the extractive solution to remove residues, collecting filtrate, separating the filtrate with ceramic membrane with aperture of 500nm and ultrafiltration membrane with aperture of 5000Da respectively, and concentrating at 80 deg.C and vacuum degree of-0.1 MPa to relative density of 1.10(25 deg.C) to obtain sunflower extract.

Example 2

Mixing 1kg of sunflower discs and 0.5kg of sunflower seed cakes uniformly, crushing into 20-mesh powder, adding 30kg of 95% (w/w) ethanol water solution, carrying out reflux extraction at 80 ℃ for 90 minutes, centrifuging the extracting solution to remove dregs, collecting filtrate, separating the filtrate by using a ceramic membrane with the aperture of 800nm and an ultrafiltration membrane with the aperture of 3000Da respectively, and concentrating the filtrate at the temperature of 60 ℃ and the vacuum degree of-0.2 MPa until the relative density is 1.07(25 ℃) to obtain the sunflower extract.

Example 3

Mixing 100g of sunflower discs and 5g of sunflower seed cakes uniformly, crushing into 80-mesh powder, adding 500ml of ethyl acetate, extracting at 70 ℃ for 180 minutes, centrifuging the extracting solution to remove dregs, collecting filtrate, separating the filtrate by using a ceramic membrane with the pore diameter of 1000nm and an ultrafiltration membrane with the pore diameter of 10000Da respectively, and concentrating the filtrate at the temperature of 80 ℃ and the vacuum degree of-0.1 MPa until the relative density is 1.11(25 ℃), thus obtaining the sunflower extract.

Example 4

Mixing 1kg of sunflower discs and 1kg of sunflower seed cakes uniformly, crushing into 60-mesh powder, adding 40kg of 50% (w/w) butanediol aqueous solution, extracting at 60 ℃ for 60 minutes, centrifuging the extracting solution to remove dregs, collecting filtrate, separating the filtrate by using a ceramic membrane with the aperture of 500nm and an ultrafiltration membrane with the aperture of 5000Da respectively, and concentrating at the temperature of 75 ℃ and the vacuum degree of-0.1 MPa until the relative density is 1.08(25 ℃), thus obtaining the sunflower extract.

Comparative example 1

The same as example 3, except that 100g of sunflower discs and 110g of sunflower seed meal were mixed and then subjected to the following operations:

mixing 100g of sunflower discs and 110g of sunflower seed cakes uniformly, crushing into 80-mesh powder, adding 1000ml of ethyl acetate, extracting at 70 ℃ for 180 minutes, centrifuging the extracting solution to remove dregs, collecting filtrate, separating the filtrate by using a ceramic membrane with the pore diameter of 1000nm and an ultrafiltration membrane with the pore diameter of 10000Da respectively, and concentrating the filtrate at the temperature of 80 ℃ and the vacuum degree of-0.1 MPa until the relative density is 1.11(25 ℃), thus obtaining the sunflower extract.

Comparative example 2

The difference from example 2 is that the extraction centrifugation is followed by concentration:

mixing 1kg of sunflower discs and 0.5kg of sunflower seed cakes uniformly, crushing to 20-mesh powder, adding 30kg of 95% (w/w) ethanol water solution, carrying out reflux extraction at 80 ℃ for 90 minutes, centrifuging the extracting solution to remove dregs, collecting filtrate, and concentrating the filtrate at the temperature of 60 ℃ and the vacuum degree of-0.2 MPa until the relative density is 1.07(25 ℃), thus obtaining the sunflower extract.

Example 5

phase A: 10% of mineral oil; 2% of cocoa butter; vitamin E (vitamin E) 1.3%; 6% of GP2000 emulsifying wax; 2.8% of glyceryl monostearate; 4% of octadecanol;

phase B: example 2 preparation of sunflower extract 5%;

and C phase: 2% of glycerol; 2% of propylene glycol; 0.5 percent of acetic acid monoethanolamide; 0.2 percent of triethanolamine; IS-45 preservative 0.45%; the deionized water is added to make up to 100 percent.

The preparation method comprises the following steps:

1. heating the phase A oil-soluble component to 70 ℃ to completely melt the phase A oil-soluble component to obtain a material 1 for later use;

2. heating the other C-phase water-soluble component (except other components) and water to 70 ℃, and uniformly stirring to obtain a material 2 for later use;

3. and (3) adding the materials 1 and 2 into an emulsion reactor simultaneously for emulsion reaction, cooling to below 40 ℃ after the emulsion reaction is finished, adding the phase B, uniformly stirring, and cooling.

To further illustrate the specific effects of the present invention, the following experiments were performed to verify the effects of the present invention.

Effect example 1

Hyaluronidase inhibition assay:

1. reagent and apparatus

Sample preparation: the sunflower extract prepared in examples 1 to 4 was diluted with purified water to a concentration of 1%, 3%, 5% (W/W).

Hyaluronidase (I): the concentration is 500U/ml, the solution is used as it is, and the acetic acid buffer solution is used as the solvent.

Sodium hyaluronate (120 ppm molecular weight): 0.5mg/ml, one-time preparation and multiple use, and acetic acid buffer solution is used as a solvent.

Buffer: 4.8ml of solution A (0.2mol/L acetic acid: 11.55ml glacial acetic acid in 1L distilled water) and 45.2ml of solution B (0.2mol/L sodium acetate: 16.4g anhydrous sodium acetate in 1L distilled water) were mixed and diluted to 100ml to prepare an acetic acid buffer solution having a pH of 5.6.

Acetylacetone solution: 50ml of a 1.0mol/L sodium carbonate solution and 3.5ml of acetylacetone are mixed homogeneously (ready for use).

P-DAB color developing agent: 0.8g of p-dimethylaminobenzaldehyde is dissolved in 15ml of concentrated hydrochloric acid (12mol/L) and 15ml of absolute ethanol and mixed uniformly.

Calcium chloride solution (CaCl)₂Solution): 2.5 mol/L.

Sodium hydroxide solution (NaOH solution): 5 mol/L.

2. Procedure of experiment

A25 ml cuvette was taken and operated according to the procedure in Table 1, in parallel for 3 times.

TABLE 1 Hyaluronidase inhibition assay specific procedures

3. Calculation and results

Hyaluronidase inhibition (%) - (C-D) - (A-B) ]/(C-D) × 100%

In the formula:

OD value of a — (hyaluronidase + sample + sodium hyaluronate) test sample solution;

OD value of blank sample B (acetic acid buffer solution + sample + acetic acid buffer solution);

OD value of C- (hyaluronidase + distilled water + sodium hyaluronate) control solution;

d- (acetate buffer + distilled water + acetate buffer) control blank OD value.

The results are shown in Table 2 and FIGS. 1 to 2.

TABLE 2 Hyaluronidase inhibition

Application concentration

Example 1

Example 2

Example 3

Example 4

Comparative example 1

Comparative example 2

Sample concentration (1%)

45.0％±2.0

47.5％±1.8

56.1％±1.5

49.5％±2.2

13.1％±0.9

8.3％±4.2

Sample concentration (3%)

74.1％±1.5

81.4％±1.3

85.6％±2.4

70.1％±2.4

26.0％±1.2

10.6％±7.8

Sample concentration (5%)

91.2％±2.6

95.0％±2.3

96.3％±3.1

90.4％±1.6

36.5％±2.7

12.4％±6.8

As can be seen from Table 2, examples 1 to 4 of the present invention all had a good hyaluronidase inhibitory effect, and the inhibitory rate tended to increase with the increase in the application concentration. The extracts of examples 1-4 all achieved over 90% inhibition at 5% application concentration.

In comparative examples 1 and 2, the inhibition rate increased with increasing application concentration, but the effect was inferior to examples 1 to 4. In particular, the extract obtained in comparative example 2 was agglomerated and difficult to disperse and dissolve when diluted with pure water to the concentration for use in the experiment, which was also a cause of low hyaluronidase inhibition and large parallel group deviation.

Effect example 2 relief of capsaicin and lactic acid stinging

First, reduction of lactic acid stinging test

Screening lactic acid stabbing pain population: at room temperature, 20. mu.L of 10% (w/w) aqueous lactic acid solution was applied to the nasolabial sulcus, and the subjective symptoms of the subjects were asked at 0, 3, 5 and 10min, respectively, and scored by 4 points (0 point was no stinging sensation, 1 point was mild stinging, 2 point was moderate stinging, 3 point was severe stinging), and the lactic acid sting reaction was positive for 3 total points and 3 points or more. And selecting the lactic acid stabbing positive reaction as a qualified subject to be tested in the next step.

The experimental process comprises the following steps:

randomly dividing the 16 people with lactic acid stabbing positive reaction into two groups (20-50 years old, half of each male and female):

negative group: applying 30 μ L of pure water to nasolabial sulcus, and applying 20 μ L of 10% (w/w) lactic acid aqueous solution to the same part after 5 min.

Test groups: 30. mu.L of the sample solution (sunflower extract prepared in examples 1 to 4) was applied to the nasolabial folds, and 20. mu.L of a 10% (w/w) aqueous solution of lactic acid was applied to the same site after 5 min.

The lactic acid solution was applied to the nasolabial fold and scored as described above, and analyzed using SPSS 19.0 statistical software, with the results shown in table 3.

TABLE 3 reduction of lactic acid stinging results

Time	Test group	Negative group	Two sets of phase contrast (P value)
				Example 1	5.3±0.7	7.5±0.9	＜0.05
Example 2	4.8±1.2	7.1±1.1	＜0.05
				Example 3	3.6±0.6	7.6±1.5	＜0.05
Example 4	4.9±1.3	8.6±0.7	＜0.05

As can be seen from table 3, the sample liquid was effective in relieving the stinging sensation caused by lactic acid.

Experiment for reducing capsaicin stabbing pain

Capsaicin, when applied to the skin, causes dilation of the microvasculature, which results in redness and warmth of the skin, accelerates the local metabolic rate, and also stimulates the pain nerves, causing the person to feel stinging.

The experimental process comprises the following steps: adding 500mg capsaicin into 10ml 70% (w/w) ethanol, and shaking to dissolve to obtain 5% capsaicin solution. The rest of the procedures were the same as the lactic acid stinging test described above, and the results are shown in Table 4.

TABLE 4 reduction of capsaicin sting results

Time	Test group	Negative group	Two sets of phase contrast (P value)
				Example 1	8.2±0.7	10.5±1.4	＜0.05
Example 2	7.5±1.2	10.3±1.7	＜0.05
				Example 3	7.9±1.6	11.4±0.9	＜0.05
Example 4	6.3±1.1	10.6±1.3	＜0.05

The results demonstrate that the sample fluid is effective in alleviating the stinging sensation caused by capsaicin.

Effect example 3 skin repair experiment

The experimental principle is as follows:

when the skin barrier is damaged, lactic acid enters the skin, stimulating nerves, and thus causing a tingling sensation. When the skin barrier is effectively repaired, the chance of lactic acid passing through the skin barrier is reduced, thereby alleviating the stinging sensation. The method is simple, convenient, effective and high in repeatability, and is widely applied to clinical evaluation of skin repair detection and experimental research.

Screening lactic acid stabbing pain population:

the difference between the lactic acid and the lactic acid sting reducing test is that the lactic acid is smeared on two cheeks of the face. Selecting lactic acid positive sting reaction subjects for further testing.

The experimental process comprises the following steps:

negative group: blank placebo-the ingredients and preparation were the same as example 5 except that no sunflower extract was included.

Test groups: sunflower extract prepared according to example 5.

Test samples: the subjects in the test group and the negative group were asked to be positive for lactic acid stinging, and the cycle was 28 days. Subjects were asked to apply the test group product to one face and the negative group product to the other face (both groups of products are indicated by a code, and the user and the test staff were double blind controlled), once in the morning and once in the evening.

The test process comprises the following steps: the lactic acid stinging tests were performed on the first day (D0), fourteenth day (D14) and twenty-eighth day (D28) of the enrollment tests, scored as described above and analyzed using SPSS 19.0 statistical software, with the results shown in table 5.

Table 5 skin barrier repair experimental results

Time	Test group	Negative group	Two sets of phase contrast (P value)
				D0	8.2±0.5	8.3±0.6	＞0.05
D14	5.0±0.8	7.4±0.3	＜0.05
				D28	3.1±1.1	6.3±0.5	＜0.05

The results show that the lactic acid stinging scores of the test groups have statistical significance (P < 0.05) compared with the time points of the negative groups D14 and D28, and that the products prepared from the sunflower extract have the effects of repairing skin barriers and resisting allergy.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. A preparation method of a sunflower extract with a soothing and repairing effect is characterized by comprising the following steps:

(1) mixing sunflower discs and sunflower seed cakes according to a weight ratio of 1: 0.05-1, and crushing to obtain sunflower powder;

(2) adding a solvent into the sunflower powder obtained in the step (1), and extracting at the temperature of 60-80 ℃ to obtain an extracting solution; then centrifuging the extracting solution, and collecting filtrate; concentrating the filtrate after membrane separation to obtain sunflower extract;

the membrane separation in the step (2) is realized by the following steps: separating the filtrate by using a ceramic membrane with the aperture of 500 nm-1000 nm and an ultrafiltration membrane with the aperture of 3000 Da-10000 Da in sequence;

the weight ratio of the sunflower powder to the solvent in the step (2) is 1: 5-20;

the solvent in the step (2) is one or two of water, ethanol, ethyl acetate and butanediol;

the concentration in the step (2) is carried out at 60-80 ℃ and-0.1-0.2 MPa of vacuum degree, and the concentration is carried out until the relative density measured at 25 ℃ is 1.05-1.15.

2. The method for preparing sunflower extract with soothing and repairing effects as claimed in claim 1, wherein:

the particle size of the sunflower powder in the step (1) is 10-100 meshes;

the extraction time in the step (2) is 30-180 minutes.

3. A sunflower extract characterized by: prepared by the method of any one of claims 1 to 2.

4. Use of the sunflower extract according to claim 3 in the cosmetic field.

5. Use of sunflower extract according to claim 4 in the cosmetic field, characterized in that:

the addition amount of the sunflower extract accounts for 1-50% of the weight of the cosmetic;

the cosmetics are cream emulsion, film powder, jelly, aerosol or pressed powder cosmetics.

6. A soothing and repairing skin cream is characterized in that: comprises the following components in percentage by weight:

phase A: 10% of mineral oil; 2% of cocoa butter; 1.3 percent of vitamin E; 6% of emulsifying wax; 2.8% of glyceryl monostearate; 4% of octadecanol;

phase B: 1-5% of the sunflower extract of claim 3;

and C phase: 2% of glycerol; 2% of propylene glycol; 0.5 percent of acetic acid monoethanolamide; 0.2 percent of triethanolamine; supplementing deionized water to 100%;

the phase C also contains a preservative;

the addition amount of the preservative accounts for 0.45 percent of the weight of the soothing and repairing skin cream;

7. Use of sunflower extract according to claim 3 for the preparation of a medicament for inhibiting hyaluronidase or antiallergic, characterized in that:

the allergy is caused by capsaicin or lactic acid.

8. Use of the sunflower extract of claim 3 for the preparation of a hyaluronidase inhibitor.