CN109288884A - A kind of preparation method and application of the Helianthi extract with repair of releiving - Google Patents
A kind of preparation method and application of the Helianthi extract with repair of releiving Download PDFInfo
- Publication number
- CN109288884A CN109288884A CN201811541249.8A CN201811541249A CN109288884A CN 109288884 A CN109288884 A CN 109288884A CN 201811541249 A CN201811541249 A CN 201811541249A CN 109288884 A CN109288884 A CN 109288884A
- Authority
- CN
- China
- Prior art keywords
- sunflower
- extract
- helianthi extract
- phase
- helianthi
- Prior art date
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- Granted
Links
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Classifications
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- A61Q17/005—Antimicrobial preparations
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Abstract
The invention discloses the preparation methods and application of a kind of Helianthi extract with repair of releiving.This method comprises the following steps: (1) crushing after mixing floral disc of sunflower and sunflower seed grouts by weight 1:0.01~1, obtain sunflower powder；(2) solvent is added into sunflower powder, is extracted under the conditions of 60 DEG C~80 DEG C, obtains extracting solution；Then extracting solution is centrifuged, collects filtrate；Filtrate is concentrated after UF membrane again, obtains Helianthi extract.After the present invention is by rationally compounding floral disc of sunflower and sunflower seed grouts, extracted separation production, which is prepared, has effects that releive the natural extract repaired, the Helianthi extract has anti-inflammatory antibacterial and repair, body injury caused by capable of alleviating and repairing because of allergic inflammation improves the immunity of body.Therefore, Helianthi extract can be applied to allergy of releiving, repaired in the cosmetics or drug of impaired skin series.
Description
Technical field
The invention belongs to field of natural medicinal chemistry and cosmetic technical field, in particular to a kind of that there is reparation of releiving to make
The preparation method and application of Helianthi extract.
Background technique
One of sunflower (Helianthus annuus L.) oil crops important as China and China are earliest
One of alimentary crop, the historical records away from modern about 5000~6000 years, is widely used in the fields such as medication chemistry and food.Its
Oil production is only second to soybean, and cultivated area is only second to soybean and rape, ranks third, and fruit sunflower seeds also may be used in addition to being used as oil expression
Long-term consumption has extraordinary healthcare function.Squeezing for sunflower oil realizes industrialized production already at present, but by-product is such as
Sunflower plate, sunflower seed shell and grouts etc. are still under-utilized.
About the utilization of sunflower associated byproducts, it is concentrated mainly on floral disc, other less reports.Such as Suo Jinling is [raw
Object technology, 2,010 20 (2) 74-77] the water-soluble polysaccharide, [Chinese patent such as Zhao Ping are extracted from floral disc of sunflower
CN106386873A] using floral disc of sunflower as raw material, the plant-based bacteriostat that centrifugal process obtains inhibition fusarium sulphureum is proposed with water.
Guo Yi etc. [Chinese patent CN108379318A] is using floral disc of sunflower as raw material, after water lift-off gains in depth of comprehension to precipitating, sediment point
The extract for inhibiting xanthine oxidase activity Yong not be obtained after ultrafiltration and 0.45um membrane filtration.[the Chinese patents such as Xie Yong
CN107049883A] using the molten object of oil in sunflower seed oil extract sunflower oil cake, the extract of acquisition has sun-proof synergy
Effect.
Skin allergy is also known as " sensibility " skin.From a medical perspective, skin allergy is primarily referred to as when skin is by each
Kind of stimulation, causes skin the abnormal phenomenon such as red and swollen, pain of itching, decortication and allergic dermatitis occur.In green, environmental protection, advocate nature
And under the derivation of back to nature theory, natural plants are in cosmetic field using more and more extensive.Studies have shown that sunflower
Leaf, flower, floral disc, shell, root, stem marrow etc. can be used as medicine, and have good medical value.Its main chemical compositions includes sesquialter
The terpenoids such as terpene, diterpene, sesquiterpene lactone, triterpenes further include sterol, cumarin, phenols, flavonoids and volatility object
Matter etc..Wherein, chlorogenic acid, terpene, flavone compound isoreactivity substance are contained in floral disc of sunflower, these substances not only have
Fungistatic effect also has antioxidant activity.Sunflower seed contains protein 21%~30%, grease 28%~32%, and there are also abundant
VE, VB6, carrotene etc., in addition also contain carbohydrate 12.6%, cellulose 2.7%, ash content 4.4%, and microelement and
Various vitamin contents are also very high, even if squeezing the grouts after grease still remains a large amount of effective component.Therefore, make full use of to
The effective component of both day sunflower disk and grouts, for further increasing in its industrial chain corresponding economic value with very heavy
The meaning wanted.And have not yet to see the relevant report after compounded about the two in terms of antiallergy.
Therefore, it is highly desirable to invent a kind of a kind of sunflower extract with repair of releiving.
Summary of the invention
The primary purpose of the present invention is that the shortcomings that overcoming the prior art and deficiency, provide a kind of with repair of releiving
Helianthi extract preparation method.
Another object of the present invention is to provide the sunflowers with repair of releiving that the method is prepared to mention
Take object.
A further object of the present invention is to provide the applications of the Helianthi extract with repair of releiving.
The purpose of the invention is achieved by the following technical solution: a kind of Helianthi extract with repair of releiving
Preparation method and application, include the following steps:
(1) it is crushed after mixing floral disc of sunflower and sunflower seed grouts by weight 1:0.01~1, obtains sunflower powder
End；
(2) solvent is added into sunflower powder obtained in step (1), is extracted under the conditions of 60 DEG C~80 DEG C,
Obtain extracting solution；Then extracting solution is centrifuged, collects filtrate；Filtrate is concentrated after UF membrane again, sunflower is obtained and mentions
Take object.
The weight ratio of floral disc of sunflower described in step (1) and sunflower seed grouts is preferably 1:0.05~1.
The granular size of sunflower powder described in step (1) is 10~100 mesh；Preferably 20~80 mesh.
The weight ratio of sunflower powder described in step (2) and solvent is 1:1~30；Preferably 1:5~20.
Solvent described in step (2) is one of water, alcohols solvent or esters solvent or two or more mixtures；
Preferably water, ethyl alcohol, one of ethyl acetate and butanediol or two or more mixtures；More preferably concentration be 50~
The ethanol water of 95% (w/w), ethyl acetate or concentration are the butanediol aqueous solution of 50% (w/w).
The time of extraction described in step (2) is 30~180 minutes；Preferably 60~180 minutes.
UF membrane described in step (2) is realized preferably by following steps: filtrate is successively used 500nm~1000nm
The ceramic membrane in aperture and the ultrafiltration membrane in the aperture 3000Da~10000Da are separated.
Concentration described in step (2) is to be concentrated under 40 DEG C~80 DEG C, -0.1~-0.3MPa vacuum degree；It is preferred that
To be concentrated under 60 DEG C~80 DEG C, -0.1~-0.2MPa vacuum degree, be concentrated under the conditions of 25 DEG C measure it is relatively close
Spend 1.05~1.15 (preferably 1.07~1.11).
A kind of Helianthi extract is prepared by method described in any of the above embodiments.
Application of the Helianthi extract in cosmetic field.
The cosmetics are preferably the cosmetics for having allergy of releiving, repairing impaired skin, including cream emulsion, film
Powder class, thermophilic curry class, aerosol class and muffin class etc..
The additive amount of the Helianthi extract accounts for the 1~50% of cosmetics weight；Preferably account for the 1 of cosmetics weight
~5%；More preferably account for the 5% of cosmetics weight.
The cosmetics further include the acceptable matrix morphology of cosmetics.
One kind, which is releived, repairs face cream, the component including following percentage:
A phase: mineral oil 10%；Cocoa butter 2%；Tie up E (vitamin E) 1.3%；Emulsifying wax 6%；Glycerin monostearate
2.8%；Octadecanol 4%；
B phase: above-mentioned Helianthi extract 1~5%；
C phase: glycerol 2%；Propylene glycol 2%；Acetic acid single ethanol amide 0.5%；Triethanolamine 0.2%；Deionized water is supplied
To 100%.
Emulsifying wax in the A phase is preferably GP2000 emulsifying wax.
The additive amount of Helianthi extract preferably accounts for described releive and repairs the 5% of face cream weight in the B phase.
Also contain preservative in the C phase；Preferably IS-45 preservative.
The additive amount of the preservative preferably accounts for described releive and repairs the 0.45% of face cream weight.
The preparation method of face cream is repaired in described releiving, and is included the following steps:
(1) 70 DEG C are heated to after mixing each component in A phase, it is melted completely, obtains A phase mixture；
(2) 70 DEG C are heated to after mixing each component in C phase, stirs evenly, obtains C phase mixture；
(3) A phase mixture is added in C phase mixture and carries out emulsion reaction, be cooled to 40 DEG C after to be emulsified, then
The component in B phase is added, stirs evenly, is cooled to room temperature, obtain releiving and repair face cream.
The Helianthi extract inhibits hyaluronidase in preparation, and anti-inflammatory is antibacterial, improves allergic constitution or antiallergy
Drug in application.
The allergy includes allergy caused by capsaicine or lactic acid.
The Helianthi extract is preparing the application in Hyaluronidase inhibitor.
Application of the Helianthi extract as Hyaluronidase inhibitor.
The present invention sufficiently to day certain herbaceous plants with big flowers resource is utilized, that is, utilizes the effective component of both floral disc of sunflower and grouts, wherein to
Contain chlorogenic acid, terpene, flavone compound isoreactivity substance in day sunflower disk, these substances not only have fungistatic effect, also
With antioxidant activity；Sunflower seed contains protein 21%~30%, grease 28%~32%, and there are also VE, VB abundant6, recklessly
In addition radish element etc. also contains carbohydrate 12.6%, cellulose 2.7%, ash content 4.4%, and microelement and various vitamins contain
Amount is also very high, even if squeezing the grouts after grease still remains a large amount of effective component, can also therefrom extract sunflower protein, green original
Acid etc..
Human skin allergy is primarily referred to as human contact and to certain anaphylactogens causes red swelling of the skin, itches, wheal, decortication
Equal skin disorders.Skin disorder caused by allergy mainly includes contact dermatitis and berloque dermatitis.Contact dermatitis refers to skin
After contacting certain substance, erythema, oedema, pain of itching locally occurs, serious person there can be the appearance of the symptoms such as bubble, decortication.It can make skin
The substance that skin generates contact dermatitis has jewellery, watch chain, mirror holder, sandals, chemical fiber fabric, externally applied drug, chemicals, cosmetics etc..
Once finding anaphylactic article immediately it was found that there is above-mentioned symptom, and stop contacting, the people for being easy to happen contact dermatitis is more
Belong to allergic human population.Cosmetic allergic contact dermatitis cosmetics can lead to that skin is red, swollen, hot, pain, the allergic dermatitises symptom such as get blister.From
Medical angle is said, if it find that not having to preferably to certain cosmetic allergic contact dermatitis.But it sometimes but is be unable to do without in life, such as person in middle and old age
People's hair dyeing is light make-up with working women.At this moment most scientific method is fundamentally to improve allergic constitution.Therefore, mainstream is anti-at present
Allergy method is to eliminate or alleviate scytitis, improves body defenses function, improves environment far from allergy source.In the present invention
Helianthi extract can effectively repair skin barrier, body injury caused by alleviating because of allergic inflammation, improve the immune of body
Power.
The present invention has the following advantages and effects with respect to the prior art:
1, after the present invention is by rationally compounding floral disc of sunflower and sunflower seed grouts, extracted separation production is prepared
A kind of natural extract repaired that has effects that releive.The present invention is using having the function of that anti-inflammatory is antibacterial in floral disc in sunflower
Chlorogenic acid, flavonoids isoreactivity object, play and inhibit the release of the anaphylactic inflammatory reaction factor；Using in sunflower seed grouts
The ingredients such as VE having repairing effect, carrotene, polysaccharide, body injury caused by alleviating and repairing because of allergic inflammation, improve machine
The immunity of body.
2, proved through Efficacy experiments, extract of the present invention have it is significant inhibit hyaluronic acid enzyme effect, inhibiting rate with
Application concentration increase and increase；Can releive shouting pain caused by capsaicine and lactic acid simultaneously；It uses through human body containing of the invention
Show that extract of the present invention can obviously repair the barrier function of impaired skin after repairing face cream 28 days.
3, extracting method of the invention is easy to operate, with short production cycle, and the extract of acquisition can be applied to have and releive
It is corresponding in its industrial chain for further increasing in cosmetics that are quick, repairing impaired skin series (additive amount is 1%~50%)
Economic value have great importance.
Detailed description of the invention
Fig. 1 hyaluronidase inhibitory effect figure under different application concentration for the Helianthi extract in Examples 1 to 4.
Fig. 2 is that the Helianthi extract in Examples 1 to 4 is shown with using the increase hyaluronidase inhibitory effect of concentration
Write reinforcing effect figure.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus；Unless
It illustrates, agents useful for same and raw material of the present invention can pass through commercially available acquisition.
Embodiment 1
By the commercially available floral disc of sunflower of 1kg (commercially available floral disc of sunflower is the commodity that dry after removing fruit) and 0.1kg to day
Sunflower seed grouts (Shandong Qiao Qi food science and technology Co., Ltd, similarly hereinafter) are crushed to 40 mesh powders after being mixed, 50% (w/ of 25kg is added
W) ethanol water, 60 DEG C are extracted 120 minutes, and extracting solution collects filtrate after being centrifuged off the dregs of a decoction, and filtrate uses the aperture 500nm respectively
Ceramic membrane and the aperture 5000Da Ultra filtration membrane after be concentrated into relative density under 80 DEG C of temperature, -0.1MPa vacuum degree
1.10 (25 DEG C) to get arrive Helianthi extract.
Embodiment 2
It is crushed to 20 mesh powders after 1kg floral disc of sunflower and 0.5kg sunflower seed grouts are mixed, 30kg 95% is added
(w/w) ethanol water, 80 DEG C refluxing extraction 90 minutes, extracting solution collects filtrate after being centrifuged off the dregs of a decoction, and filtrate is used respectively
Phase is concentrated under 60 DEG C of temperature, -0.2MPa vacuum degree after the ceramic membrane in the aperture 800nm and the Ultra filtration membrane in the aperture 3000Da
To density 1.07 (25 DEG C) to get arrive Helianthi extract.
Embodiment 3
It is crushed to 80 mesh powders after 100g floral disc of sunflower and 5g sunflower seed grouts are mixed, 500ml acetic acid second is added
Ester, 70 DEG C are extracted 180 minutes, and extracting solution collects filtrate after being centrifuged off the dregs of a decoction, and filtrate uses the ceramic membrane in the aperture 1000nm respectively
With relative density 1.11 (25 is concentrated into after the Ultra filtration membrane in the aperture 10000Da under 80 DEG C of temperature, -0.1MPa vacuum degree
DEG C) to get arrive Helianthi extract.
Embodiment 4
It is crushed to 60 mesh powders after 1kg floral disc of sunflower and 1kg sunflower seed grouts are mixed, 50% (w/ of 40kg is added
W) butanediol aqueous solution, 60 DEG C are extracted 60 minutes, and extracting solution collects filtrate after being centrifuged off the dregs of a decoction, and filtrate uses the hole 500nm respectively
Relative density is concentrated under 75 DEG C of temperature, -0.1MPa vacuum degree after the ceramic membrane of diameter and the Ultra filtration membrane in the aperture 5000Da
1.08 (25 DEG C) to get arrive Helianthi extract.
Comparative example 1
With embodiment 3, difference is to carry out subsequent behaviour after being mixed 100g floral disc of sunflower and 110g sunflower seed grouts
Make:
It is crushed to 80 mesh powders after 100g floral disc of sunflower and 110g sunflower seed grouts are mixed, 1000ml acetic acid is added
Ethyl ester, 70 DEG C are extracted 180 minutes, and extracting solution collects filtrate after being centrifuged off the dregs of a decoction, and filtrate uses the ceramics in the aperture 1000nm respectively
Relative density 1.11 (25 is concentrated into after film and the Ultra filtration membrane in the aperture 10000Da under 80 DEG C of temperature, -0.1MPa vacuum degree
DEG C) to get arrive Helianthi extract.
Comparative example 2
With embodiment 2, difference is to be concentrated after extracting centrifugation:
It is crushed to 20 mesh powders after 1kg floral disc of sunflower and 0.5kg sunflower seed grouts are mixed, 30kg 95% is added
(w/w) ethanol water, 80 DEG C refluxing extraction 90 minutes, extracting solution collects filtrate after being centrifuged off the dregs of a decoction, and filtrate is in 60 DEG C of temperature
Relative density 1.07 (25 DEG C) is concentrated under degree, -0.2MPa vacuum degree to get Helianthi extract is arrived.
Embodiment 5
One kind, which is releived, repairs face cream, is grouped as by the group of following percentage:
A phase: mineral oil 10%；Cocoa butter 2%；Tie up E (vitamin E) 1.3%；GP2000 emulsifying wax 6%；Monostearate is sweet
Grease 2.8%；Octadecanol 4%；
B phase: the Helianthi extract 5% that embodiment 2 prepares；
C phase: glycerol 2%；Propylene glycol 2%；Acetic acid single ethanol amide 0.5%；Triethanolamine 0.2%；IS-45 preservative
0.45%；Deionized water complements to 100%.
Preparation method:
1. A phase oil-soluble constitents is taken to be heated to 70 DEG C, melt it completely, obtains material 1, it is spare；
2. separately C phase water-soluble component (other compositions in addition to water) and water is taken to be heated to 70 DEG C together, stirs evenly, obtain
Material 2, it is spare；
3. material 1 and 2 to be added in emulsion reactor simultaneously and carry out emulsion reaction, be cooled to after emulsification 40 DEG C with
Lower addition B phase, stirs evenly, cooling.
To further illustrate that the specific effect of the present invention, the present invention carry out compliance test result with following experiment.
Effect example 1
Hyaluronidase Inhibition test:
1, reagent and instrument
Sample: the Helianthi extract of Examples 1 to 4 preparation is diluted to 1%, 3%, 5% (W/W) concentration with pure water.
Hyaluronidase: concentration 500U/ml, it is ready-to-use, solvent is made with acetate buffer solution.
Sodium Hyaluronate (1,200,000 molecular weight): 0.5mg/ml, it is primary to prepare, it is used for multiple times, is made of acetate buffer solution molten
Agent.
Buffer: solution A (0.2mol/L acetic acid: 11.55ml glacial acetic acid is dissolved in 1L distilled water) 4.8ml, solution B
(0.2mol/L sodium acetate: 16.4g anhydrous sodium acetate is dissolved in 1L distilled water) 45.2ml, mixed diluting to 100ml are configured to pH
=5.6 acetate buffer solution.
Acetylacetone,2,4-pentanedione solution: 50ml 1.0mol/L sodium carbonate liquor and 3.5ml acetylacetone,2,4-pentanedione are uniformly mixed and (face with existing
With).
P-DAB color developing agent: 0.8g paradime thylaminobenzaldehyde is dissolved in 15ml concentrated hydrochloric acid (12mol/L) and 15ml dehydrated alcohol
It is uniformly mixed.
Calcium chloride solution (CaCl2Solution): 2.5mol/L.
Sodium hydroxide solution (NaOH solution): 5mol/L.
2, experimentation
25ml colorimetric cylinder is taken, by 1 step operation of table, operation repetitive 3 times.
1 hyaluronidase Inhibition test specific steps of table
3, calculating and result
Hyaluronic acid enzyme inhibition rate (%)=[(C-D)-(A-B)]/(C-D) × 100%
In formula:
The OD value of A-(hyaluronidase+sample+Sodium Hyaluronate) sample solution；
The OD value of B-(acetate buffer solution+sample+acetate buffer solution) sample blank；
The OD value of C-(hyaluronidase+distilled water+Sodium Hyaluronate) contrast solution；
The OD value of D-(acetate buffer solution+distilled water+acetate buffer solution) control blank.
As a result as shown in table 2 and Fig. 1~2.
2 hyaluronic acid enzyme inhibition rate of table
| Using concentration | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 | Comparative example 2 |
| Sample concentration (1%) | 45.0% ± 2.0 | 47.5% ± 1.8 | 56.1% ± 1.5 | 49.5% ± 2.2 | 13.1% ± 0.9 | 8.3% ± 4.2 |
| Sample concentration (3%) | 74.1% ± 1.5 | 81.4% ± 1.3 | 85.6% ± 2.4 | 70.1% ± 2.4 | 26.0% ± 1.2 | 10.6% ± 7.8 |
| Sample concentration (5%) | 91.2% ± 2.6 | 95.0% ± 2.3 | 96.3% ± 3.1 | 90.4% ± 1.6 | 36.5% ± 2.7 | 12.4% ± 6.8 |
As known from Table 2, the embodiment of the present invention 1~4 all has preferable hyaluronidase inhibitory effect, and as application is dense
The increase inhibiting rate of degree is in rising trend.In application concentration 5%, Examples 1 to 4 extract can reach 90% or more suppression
Rate processed.
In comparative example 1 and 2, though inhibiting rate rises as application concentration increases, effect is poor with respect to Examples 1 to 4.It is special
It is not extract obtained in comparative example 2, when being diluted to this experimental applications concentration with pure water, extract is agglomerating, it is difficult to disperse,
Dissolution, which is also to cause hyaluronic acid enzyme inhibition rate low and the larger reason of parallel group deviation.
Effect example 2 is releived capsaicine and lactic acid shouting pain
One, lactic acid prick test is reduced
Lactic acid shouting pain crowd screening: at room temperature, being applied to nasolabial groove for the 20 μ L of lactic acid aqueous solution of 10% (w/w), point
The subjective symptoms for not inquiring subject in 0,3,5 and 10min, being scored by 4 point-scores, (0 point is no tingling sensation, and 1 point is
Slight shouting pain, 2 points are moderate shouting pain, and 3 points are severe shouting pain), 3 period total scores and more than or equal to 3 points persons are lactic acid thorn
Pain reaction is positive.Selecting lactic acid shouting pain positive reaction is that Eligible subjects carry out next step test.
Experimentation:
Above-mentioned 16 people for having lactic acid shouting pain positive reaction are randomly divided into two groups (20~50 years old, each half of men and women):
Negative group: 30 μ L of pure water is applied to nasolabial groove, smears the 20 μ L of lactic acid aqueous solution of 10% (w/w) after 5min same
Position.
Test group: sample liquid (Helianthi extract of Examples 1 to 4 preparation) 30 μ L are applied to nasolabial groove, will after 5min
The 20 μ L of lactic acid aqueous solution of 10% (w/w) smears same position.
It scores according to the above method after lactic acid aqueous solution is applied to nasolabial groove person, uses 19.0 statistics software of SPSS
It is analyzed, as a result such as table 3.
Table 3 reduces lactic acid shouting pain result
| Time | Test group | Negative group | Two groups are compared (P value) |
| Embodiment 1 | 5.3±0.7 | 7.5±0.9 | < 0.05 |
| Embodiment 2 | 4.8±1.2 | 7.1±1.1 | < 0.05 |
| Embodiment 3 | 3.6±0.6 | 7.6±1.5 | < 0.05 |
| Embodiment 4 | 4.9±1.3 | 8.6±0.7 | < 0.05 |
As can be seen from Table 3, the tingling sensation as caused by lactic acid can be effectively relieved in sample liquid.
Two, capsaicine shouting pain experiment is reduced
Capsaicine applies on the skin, can make Marjoram Extract, leads to rubefaction, fever, and accelerate local metabolic rate,
Pain nerve can be also stimulated simultaneously, makes one to feel shouting pain.
Experimentation: take 500mg capsaicine be added 10ml 70% (w/w) ethyl alcohol in, shake up make it dissolve it is peppery to get 5%
Green pepper aqueous slkali.Remaining operation is tested with above-mentioned lactic acid shouting pain, the results are shown in Table 4.
Table 4 reduces capsaicine shouting pain result
| Time | Test group | Negative group | Two groups are compared (P value) |
| Embodiment 1 | 8.2±0.7 | 10.5±1.4 | < 0.05 |
| Embodiment 2 | 7.5±1.2 | 10.3±1.7 | < 0.05 |
| Embodiment 3 | 7.9±1.6 | 11.4±0.9 | < 0.05 |
| Embodiment 4 | 6.3±1.1 | 10.6±1.3 | < 0.05 |
As a result illustrate, the tingling sensation as caused by capsaicine can be effectively relieved in sample liquid.
The experiment of 3 skin repair of effect example
Experimental principle:
When skin barrier is impaired, after lactic acid enters skin, stimulation nerve, to generate tingling sensation.When skin barrier is effective
After reparation, lactic acid is reduced by the probability of skin barrier, so that tingling sensation mitigates.This method is simple and effective, repeatable strong, quilt
It is widely used in the detection of clinical evaluation skin repair and experimental study.
Lactic acid shouting pain crowd screening:
With above-mentioned reduction lactic acid prick test, difference is that this experiment lactic acid is applied to two cheek of face.Select lactic acid positive thorn
Pain reaction subject carries out next step test.
Experimentation:
Negative group: blank placebo-is in addition to without Helianthi extract, remaining ingredient and preparation method are the same as embodiment 5.
Test group: Helianthi extract prepared by embodiment 5.
Test sample: the subject of test group and negative group requires to be lactic acid shouting pain positive, 28 days periods.It is required that by
Examination person one side face smears test set product, another side face using negative set product (two set products indicate with code name, user and
Testing staff's double blind control), once in the morning and once at night.
Experiment process: respectively at first day (D0) of selected test, fortnight (D14) and the 28th day (D28) into
Row lactic acid prick test, scores according to the above method, is analyzed using 19.0 statistics software of SPSS, as a result such as table 5.
5 skin barrier reparative experiment result of table
| Time | Test group | Negative group | Two groups are compared (P value) |
| D0 | 8.2±0.5 | 8.3±0.6 | > 0.05 |
| D14 | 5.0±0.8 | 7.4±0.3 | < 0.05 |
| D28 | 3.1±1.1 | 6.3±0.5 | < 0.05 |
As a result illustrate, the lactic acid shouting pain scoring and the comparison at negative group D14 and D28 time point of test group have statistics meaning
Adopted (P < 0.05) illustrates to have containing product made of Helianthi extract of the present invention and repairs skin barrier, anti-sensitizing effect.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of preparation method and application of the Helianthi extract with repair of releiving, which is characterized in that including as follows
Step:
(1) it is crushed after mixing floral disc of sunflower and sunflower seed grouts by weight 1:0.01~1, obtains sunflower powder；
(2) solvent is added into sunflower powder obtained in step (1), extracts, obtains under the conditions of 60 DEG C~80 DEG C
Extracting solution；Then extracting solution is centrifuged, collects filtrate；Filtrate is concentrated after UF membrane again, obtains Helianthi extract.
2. the preparation method and application of the Helianthi extract with repair of releiving according to claim 1, special
Sign is:
The weight ratio of floral disc of sunflower described in step (1) and sunflower seed grouts is 1:0.05~1；
The weight ratio of sunflower powder described in step (2) and solvent is 1:1~30；
Solvent described in step (2) is one of water, alcohols solvent or esters solvent or two or more mixtures；
UF membrane described in step (2) is achieved by the steps of: filtrate is successively used to the ceramics in the aperture 500nm~1000nm
Film and the ultrafiltration membrane in the aperture 3000Da~10000Da are separated；
Concentration described in step (2) is to be concentrated under 40 DEG C~80 DEG C, -0.1~-0.3MPa vacuum degree.
3. the preparation method and application of the Helianthi extract with repair of releiving according to claim 2, special
Sign is:
The weight ratio of sunflower powder described in step (2) and solvent is 1:5~20；
Solvent described in step (2) is water, ethyl alcohol, one of ethyl acetate and butanediol or two or more mixtures；
Concentration described in step (2) is to be concentrated under 60 DEG C~80 DEG C, -0.1~-0.2MPa vacuum degree, is concentrated into
The relative density 1.05~1.15 measured under the conditions of 25 DEG C.
4. the preparation method and application of the Helianthi extract with repair of releiving according to claim 1, special
Sign is:
The granular size of sunflower powder described in step (1) is 10~100 mesh；
The time of extraction described in step (2) is 30~180 minutes.
5. a kind of Helianthi extract, it is characterised in that: be prepared by the described in any item methods of Claims 1 to 4.
6. application of the Helianthi extract described in claim 5 in cosmetic field.
7. application of the Helianthi extract according to claim 6 in cosmetic field, it is characterised in that:
The additive amount of the Helianthi extract accounts for the 1~50% of cosmetics weight；
The cosmetics are cream emulsion, film powder class, thermophilic curry class, aerosol class or muffin class cosmetics.
8. one kind, which is releived, repairs face cream, it is characterised in that: the component including following percentage:
A phase: mineral oil 10%；Cocoa butter 2%；Vitamin e1 .3%；Emulsifying wax 6%；Glycerin monostearate 2.8%；18
Alkanol 4%；
B phase: Helianthi extract 1~5% described in claim 5；
C phase: glycerol 2%；Propylene glycol 2%；Acetic acid single ethanol amide 0.5%；Triethanolamine 0.2%；Deionized water complements to
100%；
Also contain preservative in the C phase；
The additive amount of the preservative is to account for described releive to repair the 0.45% of face cream weight；
The preparation method of face cream is repaired in described releiving, and is included the following steps:
(1) 70 DEG C are heated to after mixing each component in A phase, it is melted completely, obtains A phase mixture；
(2) 70 DEG C are heated to after mixing each component in C phase, stirs evenly, obtains C phase mixture；
(3) A phase mixture is added in C phase mixture and carries out emulsion reaction, 40 DEG C are cooled to after to be emulsified, is added
Component in B phase, stirs evenly, and is cooled to room temperature, and obtains releiving and repairs face cream.
9. Helianthi extract described in claim 5 inhibits hyaluronidase in preparation, anti-inflammatory is antibacterial, improves allergic constitution,
Or the application in antianaphylactic drug, it is characterised in that:
The allergy is allergy caused by capsaicine or lactic acid.
10. Helianthi extract described in claim 5 is preparing the application in Hyaluronidase inhibitor.
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| CN113234528A (en) * | 2021-04-29 | 2021-08-10 | 张世华 | Preparation method of sunflower disc extract |
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