CN106508971A - Sectinesterase bacteriostatic agent, preparation method and application thereof in preventing and treating pythium solani - Google Patents
Sectinesterase bacteriostatic agent, preparation method and application thereof in preventing and treating pythium solani Download PDFInfo
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- CN106508971A CN106508971A CN201610789758.7A CN201610789758A CN106508971A CN 106508971 A CN106508971 A CN 106508971A CN 201610789758 A CN201610789758 A CN 201610789758A CN 106508971 A CN106508971 A CN 106508971A
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- Prior art keywords
- sunflower
- floral disc
- bacteriostatic agent
- solvent
- sectinesterase
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a sectinesterase bacteriostatic agent. The sectinesterase bacteriostatic agent is prepared from a sectinesterase extract which is obtained by taking sectinesterase as a raw material and adopting a solvent for extracting, wherein the solvent is water. A preparation method comprises the following steps of (1) smashing the sectinesterase, thus obtaining sectinesterase powder; (2) mixing the sectinesterase powder with the solvent, and extracting, thus obtaining an extracting solution, wherein the solvent is water; (3) centrifuging the extracting solution obtained in the step (2), thus obtaining supernatant, i.e., the sectinesterase bacteriostatic agent. Application of the sectinesterase bacteriostatic agent in inhibiting pythium solani is characterized in that the mass concentration of sectinesterase water extract is 40 to 640 mg/mL and the bacteriostasis rate is up to 15.22 percent to 52.95 percent.
Description
Technical field
The present invention relates to a kind of novel agrochemical and preparation method and application, and in particular to a kind of floral disc of sunflower extract and
Its preparation method and the application for preventing and treating dry rot of potato.
Background technology
Dry rot is one of potato Common Diseases, is the main disease for causing potato tubers to lose after adopting
(Antifungal Activity of the Essential Oil of Zanthoxylum bungeanum and its
Major Constituent on Fusarium sulphureum and Dry Rot of Potato Tubers[J].Li
Xing-dong,Phytoparasitica,2014,42(4):509-517), disease symptom mainly contaminates stem tuber early stage
Only change brown in local is slightly concave falls into, and after expansion, many gauffers occurs in disease portion, in concentric wheel stripe shape, grows sometimes linen suede thereon
Shape particle.Rot to account for 88.5% (Dingxi, Gansu potato tubers of disease potato by the stem tuber that dry rot of potato causes
Separation identification [J] of dry rot disease fungus, He Suqin, Yunnan Prov Agriculture University's journal, 2004,19 (5):550-552), seriously
The yield and quality of potato is affected, and huge economic loss is brought to potato planting industry.
In actual production, the Potato Cultivars of anti-dry rot are less, mainly by adopting after kill using the fungi such as thiabendazole
Microbial inoculum is controlled to causing dry rot of potato the main pathogenic fungi fusarium sulphureum (Fusarium sulphureum).Due to
Chemical synthesis class agricultural chemicals is easily impacted to agroecological environment, and produces accumulation in crop, and then affects human health, because
And chemical synthesis class agricultural chemicals gradually decreases use in the application for prevent and treat dry rot of potato.
The content of the invention
In view of this, it is an object of the invention to provide a kind of plant-based bacteriostat, makes the floral disc of sunflower bacteriostatic agent
The fusarium sulphureum of the dry rot of potato to causing has good inhibiting effect, so as to reach to the efficient of dry rot of potato
The purpose of preventing and treating.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides floral disc of sunflower bacteriostatic agent, including for floral disc of sunflower as raw material, using solvent extraction side
The floral disc of sunflower extract that method is obtained；Described solvent is water.
Preferably, the quality of the floral disc of sunflower is 1kg with the volume ratio of solvent：(5～15) L.
Preferably, the method for the extraction is comprised the following steps：
1) floral disc of sunflower is crushed, the floral disc of sunflower powder for obtaining；
2) the floral disc of sunflower powder is mixed with solvent, extracts, obtain leaching liquor；
3) by the step 2) centrifugation of the leaching liquor that obtains, the supernatant for obtaining is floral disc of sunflower extract.
Preferably, the time of the extraction is 3～5d.
Preferably, the step 3) in centrifugation after also include：The supernatant for obtaining is concentrated and is dried.
Present invention also offers the preparation method of described floral disc of sunflower bacteriostatic agent, comprises the following steps：
1) floral disc of sunflower is crushed, the floral disc of sunflower powder for obtaining；
2) the floral disc of sunflower powder is mixed with solvent, is extracted, obtain leaching liquor, the solvent is water；
3) by the step 2) centrifugation of the leaching liquor that obtains, the supernatant for obtaining is floral disc of sunflower bacteriostatic agent.
Preferably, the step 2) in floral disc of sunflower powder before mixing with solvent cross 60～100 mesh sieves.
Preferably, the step 3) in centrifugation rotating speed be 2000～4000r/min rotating speeds；The step 3) in centrifugation
Time be 10～20min.
Present invention also offers floral disc of sunflower bacteriostatic agent prepared by described floral disc of sunflower bacteriostatic agent or methods described
Application in inhibition of potato dry rot.
Preferably, the concentration of described floral disc of sunflower bacteriostatic agent is 40～640mg/ml.
The invention provides floral disc of sunflower bacteriostatic agent, including what is obtained using solvent extraction with floral disc of sunflower as raw material
Floral disc of sunflower extract, the solvent are water.Adopt water as the active material in solvent extraction floral disc of sunflower, obtain to
Active material in day sunflower disk water extract has bacteria resistance function.
Meanwhile, the invention provides floral disc of sunflower bacteriostatic agent, the raw material sources of extraction extensively, are readily available, by inciting somebody to action
Floral disc of sunflower extracts antibacterial substance, and floral disc of sunflower is turned waste into wealth, while reducing floral disc of sunflower because burning to environment
The pollution for causing, also reduces the processing cost of floral disc of sunflower.
The present invention also provides a kind of preparation method of described floral disc of sunflower bacteriostatic agent, after floral disc of sunflower is crushed
The powder for arriving is mixed with solvent, and extraction, centrifugation, the supernatant for obtaining are mixed to get floral disc of sunflower bacteriostatic agent with auxiliary material.It is described
Preparation method has method simple, easy to operate, not high to the technical requirements of operating personnel, the reproducible spy of preparation method
Point.
The present invention also provides application of the described floral disc of sunflower bacteriostatic agent in inhibition of potato dry rot.It is described to day
Sunflower disk bacteriostatic agent can effectively suppress the growth of the fusarium sulphureum for causing dry rot of potato, dry to potato so as to reach
The inhibitory action of maize ear rot,.
Further, floral disc of sunflower bacteriostatic agent of the present invention mass concentration be 40～640mg/mL when to causing
The mutual antibacterial circle diameter every interlinkage spore of dry rot of potato is 8.82～11.05mm, and the bacteriostasis rate of fusarium sulphureum is reached
15.22%～52.95%.
Specific embodiment
The invention provides floral disc of sunflower bacteriostatic agent, including with floral disc of sunflower as raw material, using solvent extraction acquisition
Floral disc of sunflower extract, the solvent be water.
The invention provides floral disc of sunflower bacteriostatic agent, adopts water as different types of in solvent extraction floral disc of sunflower
Active material, the active material extracted in the floral disc of sunflower water extract for obtaining have bacteria resistance function.
In the present invention, the quality of the floral disc of sunflower is preferably 1kg with the volume ratio of solvent：(5～15) L, it is more excellent
Elect 1kg as：10L.
In the present invention, the method for the extraction preferably includes following steps：
1) floral disc of sunflower is crushed, obtains floral disc of sunflower powder；
2) the floral disc of sunflower powder is mixed with solvent, extracts, obtain leaching liquor；
3) by the step 2) centrifugation of the leaching liquor that obtains, the supernatant for obtaining is floral disc of sunflower extract.
In the present invention, the floral disc of sunflower is selected without insect pest, the floral disc of sunflower for going mouldy.
In the present invention, the floral disc of sunflower was preferably dried before crushing.The temperature of the drying is preferably
35～60 DEG C, more preferably 37～50 DEG C；The time of drying is preferably 1～5h, more preferably 2～4h.
In the present invention, the method for the crushing is not particularly limited, using crushing well-known to those skilled in the art
Method.In the present invention, described crushing is realized by the method for grinding.
In the present invention, the granularity of floral disc of sunflower preferably 60～100 mesh after the crushing, more preferably 65～80
Mesh.
Obtain sunflower disk meal last, the floral disc of sunflower powder is mixed with solvent by the present invention, extract, soaked
Extract.The present invention is not particularly limited to the method for the mixing, using mixed method well-known to those skilled in the art is
Can.In embodiments of the present invention, described mixing is well mixed raw material and solvent using the method for stirring.
In the present invention, the time of the extraction is 3～5d.The extraction is preferably carried out under normal temperature condition.
After obtaining leaching liquor, the present invention is centrifuged to the leaching liquor, obtains supernatant.In the present invention, it is described from
The speed of the heart is preferably 2000～4000r/min, more preferably 3500r/min.The time of the centrifugation is 10～20min, more
Preferably 15min.
After obtaining supernatant, the supernatant is preferably concentrated and is dried by the present invention, obtains floral disc of sunflower extraction
Thing pressed powder.
In the present invention, the concentration is preferably rotary distillation concentration.The temperature of described rotary distillation concentration is preferably
30～45 DEG C, more preferably 35～40 DEG C.The time of the concentration is preferably 1～5h, more preferably 2～4h.
In the present invention, described drying is preferably vacuum dried.Described vacuum drying condenser temperature is preferably -25
～-55 DEG C, more preferably -30～-45 DEG C.Described vacuum drying vacuum preferably 15～110KPa, more preferably 30
～100KPa.
In the present invention, floral disc of sunflower bacteriostatic agent preferably also includes the auxiliary material that pesticide field receives, and makes pesticide field
Formulation.
The present invention also provides a kind of preparation method of described floral disc of sunflower bacteriostatic agent, comprises the following steps：
1) floral disc of sunflower is crushed, the floral disc of sunflower powder for obtaining；
2) the floral disc of sunflower powder is mixed with solvent, is extracted, obtain leaching liquor, the solvent is water；
3) by the step 2) centrifugation of the leaching liquor that obtains, the supernatant for obtaining is floral disc of sunflower bacteriostatic agent.
In the present invention, the floral disc of sunflower powder 60～100 mesh sieves excessively before mixing with solvent, more preferably 70～
90 mesh.
In the present invention, the rotating speed of the centrifugation is preferably 2000～4000r/min, more preferably 3500r/min；It is described
The time of centrifugation is preferably 10～20min, more preferably 15min.
In the present invention, the floral disc of sunflower bacteriostatic agent for obtaining preferably also includes the auxiliary material that pesticide field receives.
In the present invention, the species of the auxiliary material that the pesticide field receives preferably includes wetting agent, and dispersant collapses
Solution agent, stabilizer, pH value regulator, defoamer, one kind in synergist and filler and several.
In the present invention, the floral disc of sunflower bacteriostatic agent is preferably made water dispersible granules or wettable powder.
Present invention also offers application of the described floral disc of sunflower bacteriostatic agent in inhibition of potato dry rot germ.
In the present invention, the mass concentration of described floral disc of sunflower bacteriostatic agent is preferably 40～640mg/ml, more preferably
For 80～320mg/mL, most preferably 160mg/mL.
With reference to embodiment to the floral disc of sunflower bacteriostatic agent for providing of the invention, preparation method and its in preventing and treating potato
Application in dry rot is described in detail, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
It is raw material to select without insect pest, the floral disc of sunflower for going mouldy, and makes powdery with pulverizer, crosses 60 mesh sieves, and collection is obtained
Floral disc of sunflower powder.Powder 1kg is weighed, is mixed with the water of 10L, extracted 5 days in adding bucket, leaching liquor is with 3000r/min
Rotating speed is centrifuged 15min, collects supernatant, and concentrated by rotary evaporation 2h under conditions of 45 DEG C, concentrate Jing vacuum freeze dryings must be extracted
Thing powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 2
It is raw material to select without insect pest, the floral disc of sunflower for going mouldy, and makes powdery with pulverizer, crosses 70 mesh sieves, and collection is obtained
Floral disc of sunflower powder.Powder 1kg is weighed, is mixed with the water of 15L, extracted 3 days in adding bucket, leaching liquor is with 3500r/min
Rotating speed is centrifuged 12min, collects supernatant, and concentrated by rotary evaporation 3h under conditions of 35 DEG C, concentrate Jing vacuum freeze dryings must be extracted
Thing powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 3
It is raw material to select without insect pest, the floral disc of sunflower for going mouldy, and makes powdery with pulverizer, crosses 95 mesh sieves, and collection is obtained
Floral disc of sunflower powder.Powder 1kg is weighed, is mixed with the water of 5L, extracted 4 days in adding bucket, leaching liquor is turned with 4000r/min
Speed centrifugation 10min, collects supernatant, and concentrated by rotary evaporation 4h under conditions of 30 DEG C, concentrate Jing vacuum freeze dryings obtain extract
Powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 4,5 and 6
It is dense that the floral disc of sunflower water extract powder that embodiment 1, embodiment 2 and embodiment 3 are obtained is configured to quality respectively
Spend for 40mg/mL, 80mg/mL, 160mg/mL, 320mg/mL and 640mg/mL.
Floral disc of sunflower water extract bacteriostatic test prepares 5 culture dishes containing 20mLPDA, after PDA solidifications, dense by 5
The extract of degree is respectively dropped into 1mL into different culture dishes and is coated with uniformly as test group, and the culture dish not dealt with is sky
It is white to compare.Each culture dish is inverted the mutual every Alternariaspp cake of the culture 1 week of equidistant access 3.After inoculation, all culture dishes in
24h is cultivated in 28 DEG C of constant incubators.After culture terminates, with slide measure with crossing method survey calculation each culture dish
The mean value of 3 colony diameters.
Suppression of the floral disc of sunflower water extract that embodiment 1, embodiment 2 and embodiment 3 are obtained to fusarium sulphureum is made
With being shown in Table 1.
Size of the 1 floral disc of sunflower water extract variable concentrations of table to the inhibition zone of the generation of fusarium sulphureum
As seen from the above embodiment, the invention provides floral disc of sunflower bacteriostatic agent, including with floral disc of sunflower as raw material
The floral disc of sunflower extract obtained using solvent extraction, the solvent are water.The mass concentration of floral disc of sunflower water extract
There is significant inhibitory action for 40～640mg/mL to the inhibition zone for causing the pathogen-fusarium sulphureum of dry rot of potato,
Bacteriostasis rate reaches 15.22%～52.95%, and in the concentration range, fungistatic effect gradually strengthens.From production cost and suppression
Bacterium effect double angle considers that the mass concentration of floral disc of sunflower water extract is preferably 80～320mg/mL, and bacteriostasis rate is
20.17～50.33%, when the mass concentration of floral disc of sunflower water extract is most preferably 160mg/mL, bacteriostasis rate reaches
33.50%～33.778%.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. floral disc of sunflower bacteriostatic agent, including the sunflower flower obtained with floral disc of sunflower as raw material, using solvent extraction process
Disk extract, described solvent are water.
2. floral disc of sunflower bacteriostatic agent according to claim 1, it is characterised in that the quality of the floral disc of sunflower with it is molten
The volume ratio of agent is 1kg：(5～15) L.
3. floral disc of sunflower bacteriostatic agent according to claim 1 and 2, it is characterised in that the method for the extraction include with
Lower step：
1) floral disc of sunflower is crushed, the floral disc of sunflower powder for obtaining；
2) the floral disc of sunflower powder is mixed with solvent, extracts, obtain leaching liquor；
3) by the step 2) centrifugation of the leaching liquor that obtains, the supernatant for obtaining is floral disc of sunflower extract.
4. floral disc of sunflower bacteriostatic agent according to claim 3, it is characterised in that the time of the extraction is 3～5d.
5. floral disc of sunflower bacteriostatic agent according to claim 3, it is characterised in that the step 3) in centrifugation after also wrap
Include：The supernatant for obtaining is concentrated and is dried.
6. the preparation method of the floral disc of sunflower bacteriostatic agent described in Claims 1 to 5 any one, it is characterised in that include with
Lower step：
1) floral disc of sunflower is crushed, the floral disc of sunflower powder for obtaining；
2) the floral disc of sunflower powder is mixed with solvent, is extracted, obtain leaching liquor, the solvent is water；
3) by the step 2) centrifugation of the leaching liquor that obtains, the supernatant for obtaining is floral disc of sunflower bacteriostatic agent.
7. preparation method according to claim 6, it is characterised in that the step 2) in floral disc of sunflower powder with
60～100 mesh sieves are crossed before solvent mixing.
8. preparation method according to claim 6, it is characterised in that the step 3) in centrifugation rotating speed be 2000～
4000r/min rotating speeds；The step 3) in centrifugation time be 10～20min.
9. described in floral disc of sunflower bacteriostatic agent described in Claims 1 to 5 any one or claim 6～8 any one
The application of floral disc of sunflower bacteriostatic agent prepared by method in inhibition of potato dry rot.
10. application according to claim 9, it is characterised in that the antibacterial agent concentration of described floral disc of sunflower be 40～
640mg/ml。
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789758.7A CN106508971B (en) | 2016-08-31 | 2016-08-31 | Floral disc of sunflower bacteriostatic agent, preparation method and its application in prevention and treatment dry rot of potato |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789758.7A CN106508971B (en) | 2016-08-31 | 2016-08-31 | Floral disc of sunflower bacteriostatic agent, preparation method and its application in prevention and treatment dry rot of potato |
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| CN106508971A true CN106508971A (en) | 2017-03-22 |
| CN106508971B CN106508971B (en) | 2018-12-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109288884A (en) * | 2018-12-17 | 2019-02-01 | 广州诗美化妆品有限公司 | A kind of preparation method and application of the Helianthi extract with repair of releiving |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1490339A (en) * | 2002-10-19 | 2004-04-21 | 王丽华 | Pectin preparation by biochemical method |
| WO2012024244A1 (en) * | 2010-08-14 | 2012-02-23 | University Of Idaho | Compositions and methods for inhibiting potato pathogens |
| CN102924622A (en) * | 2012-11-19 | 2013-02-13 | 温雪 | Helianthus annuus polysaccharide and preparation method thereof |
| CN103808838A (en) * | 2014-02-14 | 2014-05-21 | 甘肃农业大学 | Extraction purification and detection analysis method for trichothecenes in potato tuber suffering dry rot |
| CN104087639A (en) * | 2014-07-08 | 2014-10-08 | 吴庆林 | Method for extracting micromolecular active substances from sunflower calathide |
-
2016
- 2016-08-31 CN CN201610789758.7A patent/CN106508971B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1490339A (en) * | 2002-10-19 | 2004-04-21 | 王丽华 | Pectin preparation by biochemical method |
| WO2012024244A1 (en) * | 2010-08-14 | 2012-02-23 | University Of Idaho | Compositions and methods for inhibiting potato pathogens |
| CN102924622A (en) * | 2012-11-19 | 2013-02-13 | 温雪 | Helianthus annuus polysaccharide and preparation method thereof |
| CN103808838A (en) * | 2014-02-14 | 2014-05-21 | 甘肃农业大学 | Extraction purification and detection analysis method for trichothecenes in potato tuber suffering dry rot |
| CN104087639A (en) * | 2014-07-08 | 2014-10-08 | 吴庆林 | Method for extracting micromolecular active substances from sunflower calathide |
Non-Patent Citations (1)
| Title |
|---|
| 梁雪钰 等: "向日葵化学成分和药理活性研究概况", 《内蒙古医学院学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109288884A (en) * | 2018-12-17 | 2019-02-01 | 广州诗美化妆品有限公司 | A kind of preparation method and application of the Helianthi extract with repair of releiving |
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