CN104351231A - Application of 4-chlorocinnamaldehyde thiosemicarbazone in plant disease control - Google Patents
Application of 4-chlorocinnamaldehyde thiosemicarbazone in plant disease control Download PDFInfo
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Abstract
The invention relates to a novel application of 4-chlorocinnamaldehyde thiosemicarbazone in plant disease control. 4-chlorocinnamaldehyde thiosemicarbazone is taken as an active ingredient of a novel bactericide, is processed into emulsifiable concentrate or a suspending agent and used for spray treatment or seed treatment, better intake transportation activity of the active ingredient is played, and the problems of single medicament, unstable control effect, high control cost and the like of wheat root disease control in the current production as well as serious drug resistance in vegetable gray mold control are effectively solved. The control effect of the novel bactericide on three plant diseases including gaeumammomyces graminis var. tritici, wheat sharp eyespot and vegetable gray mold are researched by means of methods of indoor biological test and greenhouse and field efficacy tests. The bactericide is used for guaranteeing safe emergence of seedlings and healthy and strong seedlings after wheat seed treatment, and has good control effects on root diseases including gaeumammomyces graminis var. tritici, wheat sharp eyespot and the like, a yield increase function, characteristics of safety, effectiveness, economic efficiency as well as wide market application prospect.
Description
Technical field
The present invention relates to agricultural plant protection field, specifically, relate to the application of 4-chlorocinnamaldehyde thiosemicarbazones in controlling plant diseases.
Background technology
Take-all (Gaeumammomyces graminis var.tritici) is the important root disease of wheat, main harm wheat root and basal part of stem.According to statistics, hypopathia field underproduction 10-30%, the seriously underproduction more than 50%, even has no harvest, and is one of destructive disease in Wheat Production.Current China there is no the wheat breed of anti-full rot, although the cultivation step such as crop rotation, the rotation of crops has certain effect to preventing and treating full rot, because arable area limit is poor with operability, is difficult to large scale application.According to (Wang Gang such as Wang Gang; poplar be. Pseudomonas fluorescence P2-5 bacterial strain is to the inhibitory action [J] of take-all. plant protection; 2004; 30 (4)); although biological control method has certain inhibitory action to the expansion of Pathogen of Take-all in plant body; but can not suppress the intrusion of pathogen, therefore the method can not reduce the incidence of disease of full rot.The control of current take-all is still based on chemical measure, main use triazole bactericidal agent and Silthiopham (total eclipse is clean) carry out soil or seed treatment, but the safety of triazole bactericidal agent is poor, easily cause poisoning, cause emerge uneven, and though Silthiopham has good preventive effect, because cost is very high, its application is aborning restricted.
Wheat sharp eyespot causes primarily of Rhizoctonia cerealis (Rhizoctonia cerealis), has in recent years become the regular incidence evil of the main Mai Qu of China.Wheat, by after pathogen infection, there will be the symptoms such as rotten bud, the rotten stem of withered, the colored stalk of sick seedling, withered strain dead ears in the different bearing stage.Wheat sharp eyespot morbidity early time underproduction 20-40%, withered strain dead ears of serious formation or No kernels or seeds are gathered, as in a year of scarcity.Jinggangmycin A is mainly applied in the control of current wheat sharp eyespot or the triazole bactericidal agent such as triazolone, Triadimenol is prevented and treated at premorbid or their early stage spraying, but above-mentioned bactericide has used for many years in China's Wheat Production, because its control efficiency of resistance problems obviously declines, preventive effect is unstable, and doublely can not control other root diseases of wheat, there is certain limitation in using.
Gray mold on the vegetables such as tomato is a kind of worldwide disease caused primarily of the pathogen of Botrytis cinerea (Botrytis cinerea); main harm fruit; bamboo telegraph after morbidity; great threat is caused to tomato production, especially Winter-Spring stubble tomato in greenhouse, because of low temperature and high relative humidity; climatic variation is large; often be injured serious, generally cause tomato underproduction 20-30%, time serious, reach 50%.20 century 70s rise, benzimidazole, diformamide class and anilino-pyrimidine series bactericidal agent are used to the control of gray mold in succession, but along with widely using of these medicaments, the pathogen of Botrytis cinerea creates pesticide resistance in various degree respectively to it, the effectiveness of bactericide is reduced greatly.Therefore, be necessary that the bactericide of development of new mechanism of action puts goods on the market.
Summary of the invention
The object of this invention is to provide the novelty teabag of 4-chlorocinnamaldehyde thiosemicarbazones in controlling plant diseases.
Another object of the present invention is to provide a kind of novel bactericide and application thereof.
In order to realize the object of the invention, 4-chlorocinnamaldehyde thiosemicarbazones provided by the invention is a kind of novel (thiosemicarbazone) compound, and former medicine is yellow powder, and purity is 97%.The insecticide of to be a class with insect tyrosinase be target for template carry out structure of modification after develop the new compound of synthesis.Early-stage Study shows it and has good in vitro inhibit activities to various plants disease fungus, and infers that it has brand-new mechanism of action, and in addition, this compou nd synthesis route is simple, and production cost is lower, is the new type bactericide that a class has applications well prospect.
The application of 4-chlorocinnamaldehyde thiosemicarbazones provided by the invention in controlling plant diseases.Described plant disease comprises take-all, wheat sharp eyespot and eliminating vegetable botrytis (such as, graw mold of tomato) etc.
Present invention also offers the fungicide preparation containing 4-chlorocinnamaldehyde thiosemicarbazones, its active component is 4-chlorocinnamaldehyde thiosemicarbazones, optionally be aided with filler and/or auxiliary agent, the various formulations of processing for dressing seed or soaking seed, as suspending agent, aqueous emulsion, microemulsion, missible oil, wetting powder, water dispersible granules, micro-capsule suspension etc.Preferably make floating type seed coat agent and carry out seed pelleting process.
Described filler includes but not limited to bentonite, white carbon, Magnesiumaluminumsilicate etc., and described auxiliary agent includes but not limited to xanthans, wetting agent, dispersant, defoamer, antifreezing agent, warning colouration etc.
Wherein, described wetting agent includes but not limited to NP-10 (polyoxyethylene nonylphenol ether), OP-10 (alkylphenol polyoxyethylene), EFW (alkylnaphthalene sulfonate) etc., dispersant includes but not limited to D-425 (sodium salt of alkyl naphthalene sulfonic acid condensation polymer), D-450 (sulfonate of alkyl naphthalene sulfonic acid condensation polymer) etc., defoamer includes but not limited to n-octyl alcohol, organosilicon SAG1522 etc., antifreezing agent includes but not limited to ethylene glycol etc., and warning colouration includes but not limited to basic rhodamine, bronze red 3110, sun-proof pink 3228 etc.
The present invention also provides the application of described bactericide in controlling plant diseases.Described plant disease comprises take-all, wheat sharp eyespot and eliminating vegetable botrytis (such as, graw mold of tomato).
The mass percentage of active component is that the bactericide of 0.8-10% carries out dressing or Dressing to seed by aforesaid application; Wherein, the mass ratio of described bactericide and seed is 1:30-80.
Aforesaid application, by the mass percentage of active component be the bactericide of 1-25% be watered dilution 100-1000 doubly after, the root of plant, stem, leaf are carried out spraying and are processed.Preferably, 4-chlorocinnamaldehyde thiosemicarbazones is mixed with 25% missible oil, after being watered dilution 500 times, carries out foliar spray.
New type bactericide provided by the invention, its active component is 4-chlorocinnamaldehyde thiosemicarbazones.4-chlorocinnamaldehyde thiosemicarbazones is processed into missible oil or suspending agent, for process or the seed treatment of spraying, play its good Uptake and translocation active, efficiently solve wheat root disease pesticide control in producing at present single, preventive effect is unstable, the problems such as cost accounting is high, and resistance problems serious in eliminating vegetable botrytis control.The present invention also by indoor bioassay and greenhouse and field efficacy study on determination method new type bactericide to the inhibit activities of take-all, wheat sharp eyespot and eliminating vegetable botrytis and control efficiency.This bactericide is for safety of emerging after wheat seed process, and seedling is healthy and strong, and has good preventive effect to the root disease such as take-all, wheat sharp eyespot, and there is production-increasing function, in a word, this bactericide is safe, effective, economical, has wide market application foreground.
Accompanying drawing explanation
Fig. 1 be in the embodiment of the present invention 1 4-chlorocinnamaldehyde thiosemicarbazones to the mycelial growth inhibitory action result of rhizoctonia cerealis.
Fig. 2 is that in the embodiment of the present invention 2,4-chlorocinnamaldehyde thiosemicarbazones prevents and treats take-all and wheat sharp eyespot field control effectiveness test photo.
Fig. 3 is 4-chlorocinnamaldehyde thiosemicarbazones control graw mold of tomato greenhouse test of pesticide effectiveness photo in the embodiment of the present invention 3.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art, is raw materials usedly commercial goods.
Embodiment 1 4-chlorocinnamaldehyde thiosemicarbazones is to the bacteriostatic activity of gaeumannomyces graminis and Rhizoctonia cerealis
One, materials and methods
1.1 for examination material
1.1.1 strains tested
Take-all bacteria strain (Gaeumammomyces graminis var.tritici) XX1 (being provided by China Agricultural University)
Wheat sharp eyespot bacteria strain (Rhizoctonia cerealis) RC1 (being provided by China Agricultural University)
1.1.2 reagent agent
4-chlorocinnamaldehyde thiosemicarbazones (purity is the yellow powder of 97%, is provided by college of science of China Agricultural University)
1.1.3 the preparation of medium
The preparation of potato dextrose medium (PDA): claim peeled potatoes 200g to cut fritter, the 1000ml that adds water boils half an hour, with gauze elimination potato ball, add water and supply 1000ml, then glucose 18g and agar-agar 15g is added, after heating makes agar-agar melt completely, by filtered through gauze, packing sterilizing, for subsequent use.
1/5PDA medium preparing: the same with PDA preparation method, difference only potato content is 1/5 of PDA medium, and namely 1000mL medium adds 40g potato.
1.2 test method
1.2.1 the cultivation of pathogen
Gaeumannomyces graminis is inoculated in 1/5PDA flat board, rhizoctonia cerealis is inoculated in PDA flat board, be placed in 25 DEG C of incubators (gaeumannomyces graminis) and 12 DEG C of (rhizoctonia cerealis) dark culturing respectively, cultivate 5d and 10d respectively for subsequent use.
1.2.2 Toxicity Determination method
Reagent agent dimethyl sulfoxide (DMSO) being mixed with concentration is 1 × 10
5the mother liquor of μ g/ml, is stored in 4 DEG C of refrigerators, when carrying out toxicity test, is diluted to 8 series concentration gradients with dimethyl sulfoxide (DMSO), as shown in table 1.
Drug concentration after table 1 dilutes
With liquid-transfering gun draw each series concentration medicament 60 μ l add respectively sterilized be cooled to 45 DEG C 60ml 1/5PDA (for gaeumannomyces graminis cultivate) or PDA medium (for rhizoctonia cerealis cultivate) in, after mixing, pouring 1/5PDA or the PDA medium of band medicine into diameter is in the culture dish of 9cm, every ware 15mL, each drug concentration repeats for 4 times.The process only adding 60 μ l dimethyl sulfoxide (DMSO)s is set up to be blank.
By dull and stereotyped for the rhizoctonia cerealis of the gaeumannomyces graminis and 10d of cultivating 5d respectively, the bacterium cake that diameter is 0.5 ㎝ is prepared into along colony edge card punch, mycelia faces down and is inoculated in band medicine and blank medium respectively, gaeumannomyces graminis flat board and rhizoctonia cerealis flat board are placed in 25 DEG C of incubators and 12 DEG C of incubator dark culturing respectively, measure colony diameter respectively at after 5d and 10d, calculate mycelial growth inhibition rate.Then inhibiting rate is changed into probit value (Y), drug concentration converts denary logarithm value (X) to, X-Y is made regression line, obtain the toxicity regression curvilinear equation Y=a+bX of each medicament to gaeumannomyces graminis and rhizoctonia cerealis respectively, and correlation coefficient r, effectively suppress in concentration EC50 value and suppression 90% concentration EC90 value.
Two, Toxicity Determination result
Toxicity Determination the results are shown in Table 2.As shown in Table 2,4-chlorocinnamaldehyde thiosemicarbazones all has obvious inhibitory action to the mycelial growth of gaeumannomyces graminis, rhizoctonia cerealis.Wherein, to the EC of gaeumannomyces graminis
50be worth less, show outstanding bacteriostatic activity; To the EC of rhizoctonia cerealis
50value is 3.15, shows good bacteriostatic activity.According to EC
90value compares medicament to two kinds for the bacteriostasis trying pathogen, and showing that gaeumannomyces graminis is the most responsive to 4-chlorocinnamaldehyde thiosemicarbazones, is secondly rhizoctonia cerealis.
Table 2 4-chlorocinnamaldehyde thiosemicarbazones is to the inhibitory action of gaeumannomyces graminis and Rhizoctonia cerealis mycelial growth
The mycelial growth inhibitory action of 4-chlorocinnamaldehyde thiosemicarbazones to rhizoctonia cerealis is shown in Fig. 1.
Embodiment 2 4-chlorocinnamaldehyde thiosemicarbazones is to the field control effect of take-all and wheat sharp eyespot
One, materials and methods
1.1 for examination material
1.1.1 wheat breed
All wheats 22
1.1.2 reagent agent
Test medicine: 5%4-chlorocinnamaldehyde thiosemicarbazones suspending agent, clean (silicon plug bacterium amine, the Silthiopham) suspending agent of 12.5% total eclipse, 3% difenoconazole suspending agent.
Wherein, the preparation method of 5%4-chlorocinnamaldehyde thiosemicarbazones suspending agent is as follows: adopt wet grinding method, by the surfactant 0201B of 4%, high-purity sodium form bentonite A of 1%, 5%4-chlorocinnamaldehyde thiosemicarbazones, 0.4% warning colouration (basic rhodamine) and 2% ethylene glycol and water add successively in sand mill and grind 45 minutes, make product cut size reach less than 4.0 microns, obtain suspending agent.
Wherein total eclipse is only for preventing and treating the contrast medicament of take-all, and Difenoconazole is the contrast medicament of control wheat sharp eyespot.4-chlorocinnamaldehyde thiosemicarbazones adopts seed pelleting to process, and pharmacopoeia mass ratio is 1:30 and 1:50.Total eclipse is clean, Difenoconazole adopts dressing seed process, and wherein Difenoconazole with medicament 50ml dresses seed after adding 150ml water 10kg seed, and dress seed after the clean with medicament 20ml of total eclipse adds 180ml water 10kg.
1.2 test method
1.2.1 sample plot basic condition
Experimental field be arranged in east, Zhong Lizhuan village of Yuquan office, Jiyuan City, Henan Province.Soil is sandy loam, and water as well water pouring, preceding crop is corn.Front stubble wheat has take-all and wheat sharp eyespot morbidity record.
1.2.2 experimental scheme: than design between test adopts, four repetitions, long 8 meters of cell row, community is wide is 4 meters, and plot area is 32 square metres.
Experimental field is sowed according to the seeding quantity of 10 kgs/acre, and every community amount of broadcasting is 0.48 kilogram.
Test in sowing on October 8, October 15 emerged.Disease is repeated a situation arises investigation to four May 28 (ripening stage).
18 row are planted in each community, and in the middle of results, 3 row meters produce.
1.2.3 tested variety and test process
In table 3.
Process tested by table 3
4-chlorocinnamaldehyde thiosemicarbazones adopts 1:30 and 1:50 two pharmacopoeia ratio Cotton seeds, and all the other formulations all carry out Dressing in upper medicine administered to bring out the cold kind ratio.
1.2.4 method for treating seeds
Wheat cultivation the last week, make with medicament carry out dressing to the wheat seed after selected respectively, method is first placed in container by seed, then add seed coat agent by the pesticide-seeds ratio required by each test process, then seed and seed coating medicine are stirred, wait after drying to broadcast.Other medicaments are dressed seed in sowing the previous day, and method is the pharmaceutical quantities thin up needed for test, and evenly spray with watering can and mix on the wheat seeds after selected, then stirred by wheat seeds, vexed dry waiting is broadcast.When wheat cultivation, according to mu seeding quantity conversion community grain weight, adopt trench digging drilling method the dressing of each community and the seed mixing medicine to be sown into respectively, field management is carried out routinely.
1.2.5 wheat seeding rate and preventive effect are investigated and are calculated
Emergence rate is investigated: when wheat cultivation, and on the furrow ridge in each community, each pendulum is broadcast 300 seeds process and found seedling number, to emerge number in wheat tidy seedlings output " Invest, Then Investigate ", calculating emergence rate.
Control efficiency is investigated: in wheat ripening stage investigation take-all incidence, 5 points are got in every community, gets 1m duplicate rows statistics morbidity dead ears wheat number and wheat sum, calculates dead ears rate and relative control efficiency for often.Computing formula is with reference to " pesticide field efficacy medicine test criterion, GB/T17980.109-2004 ".
Dead ears rate (%)=(withered dead ears number/investigate total spike number) × 100
Control efficiency (%)=[(blank district dead ears rate-treatment region dead ears rate)/blank district dead ears rate] × 100 emergence rates (%)=(number of emerging/sowing number) × 100
Simultaneously in wheat ripening stage investigation wheat sharp eyespot incidence, every community diagonal 5 sampling, often some investigation 100 strains, calculate the incidence of disease and control efficiency.Computing formula is with reference to " pesticide field efficacy medicine test criterion, GB/T17980.108-2004 ".
Diseased plant rate (%)=(diseased plant number/investigation sum) × 100
Control efficiency (%)=[(blank district diseased plant rate-treatment region diseased plant rate)/blank district diseased plant rate] × 100
Wheat yield measures: survey in wheat harvest and produce, and three row in the middle of the results of every community, singly receive singles, weigh, amount to a day per mu yield according to cell production, calculate average rate of growth.
Two, field control effectiveness test result
According to relevant " pesticide field efficacy medicine test criterion " requirement of the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC, carry out the test of pesticide effectiveness in China's wheat main producing region Henan Province to evaluate the field control effect of 4-chlorocinnamaldehyde thiosemicarbazones to take-all and wheat sharp eyespot.Result shows, the seed coat agent of 4-chlorocinnamaldehyde thiosemicarbazones is according to after the pesticide-seeds ratio process wheat seed of 1:30 and 1:50, 80.0% and 60.0% are respectively to the preventive effect of take-all, 75% and 68% are respectively to the preventive effect of wheat sharp eyespot, the preventive effect of wheat sharp eyespot is better than or is equivalent to contrast medicament Difenoconazole, to the preventive effect of take-all with to contrast medicament total eclipse clean without significant difference, but be better than conventional dose in production because of 4-chlorocinnamaldehyde thiosemicarbazones use cost with to the safety of plant, therefore be the preferred agents of preventing and treating take-all.Result of the test is in table 4.
To the field efficacy of take-all and wheat sharp eyespot after the different pesticide-seeds ratio seed pelleting of table 4 4-chlorocinnamaldehyde thiosemicarbazones
In addition, by comparing the impact of different agents process on wheat seeding rate and output, find after chemicals treatment all to the safety of emerging of wheat.The impact of chemicals treatment on wheat yield the results are shown in Table 5, and test finds that all medicaments all have certain production-increasing function, and wherein 4-chlorocinnamaldehyde thiosemicarbazones 1:50 dressing production-increasing function is comparatively obvious, and contrast medicament Difenoconazole and total eclipse net phase ought.Based on the above results, 4-chlorocinnamaldehyde thiosemicarbazones 1:50 dressing all has better control efficiency to take-all and wheat sharp eyespot, and do not affect wheat seeding rate, wheat yield can be significantly improved, therefore it can be used as recommended dose to carry out control that seed pelleting prevents and treats take-all and wheat sharp eyespot, and improve yield of wheat.
To wheat seeding rate and yield effect result after the different pesticide-seeds ratio seed pelleting of table 5 4-chlorocinnamaldehyde thiosemicarbazones
Based on the above results, 4-chlorocinnamaldehyde thiosemicarbazones 1:50 dressing all has better control efficiency to take-all and wheat sharp eyespot, and do not affect wheat seeding rate, wheat yield can be significantly improved, therefore it can be used as recommended dose to carry out control that seed pelleting prevents and treats take-all and wheat sharp eyespot, and improve yield of wheat.
Take-all and wheat sharp eyespot are soil-borne diseases, and pathogen lives away from home in soil, infect morbidity from the root of plant, so suggestion adopts seed coating technology to carry out seed treatment prior to seeding, reduce the First aggression of pathogen, improve control efficiency, and then improve wheat yield.
4-chlorocinnamaldehyde thiosemicarbazones prevents and treats take-all and wheat sharp eyespot field control effectiveness test photo as shown in Figure 2.
Embodiment 3 4-chlorocinnamaldehyde thiosemicarbazones is to the control of graw mold of tomato
One, materials and methods
1.1 for examination material
1.1.1 strains tested
Strains tested: botrytis cinerea (Botrytis cinerea) bacterial strain FJ1-3 (being provided by China Agricultural University)
1.1.2 reagent agent
4-chlorocinnamaldehyde thiosemicarbazones (purity is the yellow powder of 97%, is provided by college of science of China Agricultural University)
1.1.3 the preparation of medium
The preparation of potato dextrose medium (PDA) is with embodiment 1.
1.2 test method
1.2.1 the cultivation of pathogen
Botrytis cinerea is inoculated in PDA flat board, is placed in 18 DEG C of incubator dark culturing 4d for subsequent use.
1.2.2 Toxicity Determination
Reagent agent dimethyl sulfoxide (DMSO) being configured to concentration is 1 × 10
5μ g/ml mother liquor is stored in 4 DEG C of refrigerators, when carrying out toxicity test, is diluted to series concentration gradient with dimethyl sulfoxide (DMSO), as shown in table 6.
Drug concentration after table 6 dilutes
Draw each series concentration medicament 60 μ l with liquid-transfering gun to add respectively and be sterilizedly cooled in the 60ml PDA medium of 45 DEG C, after mixing, pouring the PDA medium of band medicine into diameter is in the culture dish of 9cm, every ware 15mL, each concentration repeats for 4 times, sets up the process only adding 60 μ l dimethyl sulfoxide (DMSO)s to be blank.
By dull and stereotyped for the tomato germ PDA cultivating 4d, be prepared into along colony edge card punch the bacterium cake that diameter is 0.5cm, mycelia faces down and is inoculated in respectively in band medicine and blank medium, is placed in 18 DEG C of incubator dark culturing, after 3d, measure colony diameter, calculate mycelial growth inhibition rate.Then inhibiting rate is changed into probit value (Y), drug concentration converts denary logarithm value (X) to, X-Y is made regression line, obtain the toxicity regression curvilinear equation Y=a+bX of medicament to botrytis cinerea, and correlation coefficient r, effectively suppress in concentration EC50 value and suppression 90% concentration EC
90value.
1.2.3 the greenhouse test of pesticide effectiveness
(1) reagent agent
Reagent agent: 25%4-chlorocinnamaldehyde thiosemicarbazones missible oil, 25% iprodione suspending agent
Wherein 4-chlorocinnamaldehyde thiosemicarbazones uses with foliar spray after 500 times of dilutions, and iprodione uses with foliar spray after 500 times of dilutions.
(2) sample plot basic condition
Experimental field be arranged in the green house in Pinggu town, Pinggu district.Soil is brunisolic soil, waters as running water pouring.
(3) experimental scheme: test adopts randomized arrangement design, repeats for four times, and long 12 meters of cell row, community is wide is 2 meters, and two row tomatoes are planted in every community, and plot area is 24 square metres.
(4) test process: as shown in table 7.
Drug concentration after table 7 dilutes
On May 4th, 2014 greenhouse occur the initial incidence of disease of slight graw mold of tomato incidence " Invest, Then Investigate " and first time dispenser, dispenser again on May 10, to tomato upper bottom even spraying, the graw mold of tomato incidence of disease need be investigated May 17 and add up control efficiency during dispenser.
(5) medicament preparation method
25%4-chlorocinnamaldehyde thiosemicarbazones missible oil is prepared when there is the slight disease symptom of graw mold of tomato in field, former for 10g medicine is dissolved in 40mL dimethyl sulfoxide (DMSO), 1g wetting agent EFW and 1g dispersant MORWET D-500 is added after ultrasonic, vortex concussion is dissolved the rear medicament that takes a morsel and is added to the water, find that it is uniformly dispersed and profit is not stratified, namely can be used for field spray and use.Be watered during use and be diluted to 500 times of volumes, adopt miniaturised nebuliser to carry out foliar spray.Iprodione suspending agent is watered and is diluted to 500 times of direct foliar sprays of volume.Clear water control treatment is the same.
(6) medicament is to the investigation of graw mold of tomato preventive effect and calculating
Control efficiency is investigated: every community is determined strain and investigated 10 strains, and 5 leaf investigation are not got in every strain from top to bottom (point upper, middle and lower portion) each several part, and investigation fruit real-time investigation determines the whole fruits in strain, and the two addition is then total incidence of disease.Investigation morbidity radix before first time spray medicine, 7d Investigate incidence after last dispenser also calculates effect of chemical control.Computing formula is with reference to " pesticide field efficacy medicine test criterion, GB/T17980.120-2004 ".
Sick leaf (really) rate (%)=(sick leaf (really) count/investigates total leaf (really) number) × 100
Control efficiency (%)=(before 1-blank district medicine after sick leaf (really) rate × treatment region medicine sick leaf (really) rate)/(after blank district medicine before sick leaf (really) rate × treatment region medicine sick leaf (really) rate)) × 100
Two, result of the test
2.1 Toxicity Determination results
Toxicity Determination the results are shown in Table 8.As shown in Table 8, the mycelial growth of 4-chlorocinnamaldehyde thiosemicarbazones to botrytis cinerea shows very strong inhibitory action.Its EC
50value is 0.43 μ g/ml.
Table 8 4-chlorocinnamaldehyde thiosemicarbazones is to the inhibitory action of botrytis cinerea mycelial growth
2.2 greenhouse control graw mold of tomato effects
Greenhouse pot culture result of the test is as shown in table 9.As shown in Table 9,4-chlorocinnamaldehyde thiosemicarbazones has good control efficiency to graw mold of tomato.Wherein 25%4-chlorocinnamaldehyde thiosemicarbazones missible oil dilutes 500 times and sprays afterwards and reach 60.0% to the preventive effect of graw mold of tomato, is better than producing conventional dose iprodione.Therefore 4-chlorocinnamaldehyde thiosemicarbazones can be used for the control of graw mold of tomato, recommends field to use final concentration to be about 500 μ g/ml.Graw mold of tomato is aeroborne disease, and suggestion, when fragmentary disease symptom appears in field, is sprayed with 4-chlorocinnamaldehyde thiosemicarbazones in time, prevented the state of an illness from further developing, can gather in the crops good preventive effect.
Table 9 4-chlorocinnamaldehyde thiosemicarbazones is to the greenhouse test of pesticide effectiveness result of graw mold of tomato
Fig. 3 is shown in by 4-chlorocinnamaldehyde thiosemicarbazones control graw mold of tomato greenhouse test of pesticide effectiveness photo.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
- The application of 1.4-chlorocinnamaldehyde thiosemicarbazones in controlling plant diseases.
- 2. application according to claim 1, is characterized in that, described plant disease comprises take-all, wheat sharp eyespot and eliminating vegetable botrytis.
- 3. a bactericide, is characterized in that, its active component is 4-chlorocinnamaldehyde thiosemicarbazones, is optionally aided with filler and/or auxiliary agent.
- 4. bactericide according to claim 3, is characterized in that, described filler includes but not limited to bentonite, white carbon, Magnesiumaluminumsilicate, and described auxiliary agent includes but not limited to xanthans, wetting agent, dispersant, defoamer, antifreezing agent, warning colouration;Wherein, described wetting agent includes but not limited to NP-10, OP-10, EFW, described dispersant includes but not limited to D-425, D-450, described defoamer includes but not limited to n-octyl alcohol, organosilicon SAG1522, described antifreezing agent includes but not limited to ethylene glycol, and described warning colouration includes but not limited to basic rhodamine, bronze red 3110, sun-proof pink 3228.
- 5. bactericide according to claim 3, is characterized in that, described bactericide is suspending agent, aqueous emulsion, microemulsion, missible oil, wetting powder or water dispersible granules.
- 6. the application of bactericide in controlling plant diseases described in any one of claim 3-5.
- 7. application according to claim 6, is characterized in that, described plant disease comprises take-all, wheat sharp eyespot and eliminating vegetable botrytis.
- 8. application according to claim 7, is characterized in that, is that the bactericide of 0.8-10% carries out dressing or Dressing to seed by the mass percentage of active component; Wherein, the mass ratio of described bactericide and seed is 1:30-80.
- 9. application according to claim 7, is characterized in that, by the mass percentage of active component be the bactericide of 1-25% be watered dilution 100-1000 doubly after, the root of plant, stem, leaf are carried out spraying and are processed.
- 10. application according to claim 9, is characterized in that, 4-chlorocinnamaldehyde thiosemicarbazones is mixed with 25% missible oil, carries out foliar spray after being watered dilution 500 times.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107810682A (en) * | 2017-09-28 | 2018-03-20 | 江苏省农业科学院 | For the indoor biometricses method of wheat sharp eyespot pesticide control |
| CN107827885A (en) * | 2017-11-17 | 2018-03-23 | 昌邑市龙港无机硅有限公司 | A kind of 2,4 two cinnamoyl chloride thioureas of Pyrazolopyridine substitution and its application |
| CN115211436A (en) * | 2022-07-04 | 2022-10-21 | 江西农业大学 | Agricultural synergistic bactericide and application thereof |
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| WO2007051249A1 (en) * | 2005-11-03 | 2007-05-10 | A & C Chemicals Pty Ltd | Seed dressing |
| CN103694155A (en) * | 2012-09-27 | 2014-04-02 | 中国农业大学 | Novel substituted phenylpropionaldehyde (benzylideneacetaldehyde) thiosemicarbazone compounds and applications of the compounds as agricultural bactericides |
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| WO2007051249A1 (en) * | 2005-11-03 | 2007-05-10 | A & C Chemicals Pty Ltd | Seed dressing |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107810682A (en) * | 2017-09-28 | 2018-03-20 | 江苏省农业科学院 | For the indoor biometricses method of wheat sharp eyespot pesticide control |
| CN107810682B (en) * | 2017-09-28 | 2021-08-06 | 江苏省农业科学院 | Indoor bioassay method for wheat sharp eyespot control agent |
| CN107827885A (en) * | 2017-11-17 | 2018-03-23 | 昌邑市龙港无机硅有限公司 | A kind of 2,4 two cinnamoyl chloride thioureas of Pyrazolopyridine substitution and its application |
| CN115211436A (en) * | 2022-07-04 | 2022-10-21 | 江西农业大学 | Agricultural synergistic bactericide and application thereof |
| CN115211436B (en) * | 2022-07-04 | 2023-09-19 | 江西农业大学 | Agricultural synergistic bactericide and application thereof |
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| CN104351231B (en) | 2016-06-22 |
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