CN108456687B - 基于赖氨酸浓度控制的重组表达质粒、转化子及其应用 - Google Patents
基于赖氨酸浓度控制的重组表达质粒、转化子及其应用 Download PDFInfo
- Publication number
- CN108456687B CN108456687B CN201710094155.XA CN201710094155A CN108456687B CN 108456687 B CN108456687 B CN 108456687B CN 201710094155 A CN201710094155 A CN 201710094155A CN 108456687 B CN108456687 B CN 108456687B
- Authority
- CN
- China
- Prior art keywords
- promoter
- gene
- lysine
- repressor
- recombinant expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000004472 Lysine Substances 0.000 title claims abstract description 180
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title claims abstract description 178
- 239000013613 expression plasmid Substances 0.000 title claims abstract description 41
- 238000003259 recombinant expression Methods 0.000 title claims abstract description 41
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 269
- 230000014509 gene expression Effects 0.000 claims abstract description 130
- 108010048581 Lysine decarboxylase Proteins 0.000 claims abstract description 43
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 38
- VHRGRCVQAFMJIZ-UHFFFAOYSA-N cadaverine Chemical compound NCCCCCN VHRGRCVQAFMJIZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 230000004044 response Effects 0.000 claims abstract description 20
- 238000013518 transcription Methods 0.000 claims abstract description 11
- 230000035897 transcription Effects 0.000 claims abstract description 11
- 239000013612 plasmid Substances 0.000 claims description 52
- 108091033319 polynucleotide Proteins 0.000 claims description 44
- 102000040430 polynucleotide Human genes 0.000 claims description 44
- 239000002157 polynucleotide Substances 0.000 claims description 44
- 229920001184 polypeptide Polymers 0.000 claims description 42
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 42
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 42
- 239000012634 fragment Substances 0.000 claims description 33
- 230000001580 bacterial effect Effects 0.000 claims description 27
- 241000588724 Escherichia coli Species 0.000 claims description 23
- 238000011144 upstream manufacturing Methods 0.000 claims description 21
- 230000001939 inductive effect Effects 0.000 claims description 20
- 101150035025 lysC gene Proteins 0.000 claims description 20
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 18
- 150000003384 small molecules Chemical class 0.000 claims description 18
- 102000004031 Carboxy-Lyases Human genes 0.000 claims description 16
- 108090000489 Carboxy-Lyases Proteins 0.000 claims description 16
- 101150008667 cadA gene Proteins 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 14
- 101150041134 ldcC gene Proteins 0.000 claims description 14
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 239000003550 marker Substances 0.000 claims description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 12
- 241000588729 Hafnia alvei Species 0.000 claims description 11
- 101150109249 lacI gene Proteins 0.000 claims description 11
- 102000004317 Lyases Human genes 0.000 claims description 10
- 108090000856 Lyases Proteins 0.000 claims description 10
- 108091023045 Untranslated Region Proteins 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 9
- 239000003814 drug Substances 0.000 claims description 9
- 102000003960 Ligases Human genes 0.000 claims description 7
- 108090000364 Ligases Proteins 0.000 claims description 7
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 101150044616 araC gene Proteins 0.000 claims description 7
- 102000034287 fluorescent proteins Human genes 0.000 claims description 7
- 108091006047 fluorescent proteins Proteins 0.000 claims description 7
- 230000005764 inhibitory process Effects 0.000 claims description 7
- 239000012474 protein marker Substances 0.000 claims description 7
- 108700003859 araC Genes Proteins 0.000 claims description 6
- 102100032252 Antizyme inhibitor 2 Human genes 0.000 claims description 5
- 108091022930 Glutamate decarboxylase Proteins 0.000 claims description 5
- 102000008214 Glutamate decarboxylase Human genes 0.000 claims description 5
- 101000798222 Homo sapiens Antizyme inhibitor 2 Proteins 0.000 claims description 5
- 102000004157 Hydrolases Human genes 0.000 claims description 5
- 108090000604 Hydrolases Proteins 0.000 claims description 5
- 102000004195 Isomerases Human genes 0.000 claims description 5
- 108090000769 Isomerases Proteins 0.000 claims description 5
- 102000052812 Ornithine decarboxylases Human genes 0.000 claims description 5
- 108700005126 Ornithine decarboxylases Proteins 0.000 claims description 5
- 108090000854 Oxidoreductases Proteins 0.000 claims description 5
- 102000004316 Oxidoreductases Human genes 0.000 claims description 5
- 102000004357 Transferases Human genes 0.000 claims description 5
- 108090000992 Transferases Proteins 0.000 claims description 5
- 108010035075 Tyrosine decarboxylase Proteins 0.000 claims description 5
- 239000003102 growth factor Substances 0.000 claims description 5
- 239000005556 hormone Substances 0.000 claims description 5
- 229940088597 hormone Drugs 0.000 claims description 5
- 238000012262 fermentative production Methods 0.000 claims description 3
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 160
- 235000018977 lysine Nutrition 0.000 description 158
- 102000004169 proteins and genes Human genes 0.000 description 48
- 235000018102 proteins Nutrition 0.000 description 21
- 108020004414 DNA Proteins 0.000 description 15
- 239000000411 inducer Substances 0.000 description 14
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 11
- 108091081024 Start codon Proteins 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 9
- 229940079593 drug Drugs 0.000 description 8
- 230000006698 induction Effects 0.000 description 8
- KJOMYNHMBRNCNY-UHFFFAOYSA-N pentane-1,1-diamine Chemical compound CCCCC(N)N KJOMYNHMBRNCNY-UHFFFAOYSA-N 0.000 description 8
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 7
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 7
- 239000002207 metabolite Substances 0.000 description 7
- 108700026220 vif Genes Proteins 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 5
- 238000006114 decarboxylation reaction Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 108010034634 Repressor Proteins Proteins 0.000 description 4
- 102000009661 Repressor Proteins Human genes 0.000 description 4
- -1 antibody Substances 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 241000220317 Rosa Species 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 235000019766 L-Lysine Nutrition 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 108020004422 Riboswitch Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108010055400 Aspartate kinase Proteins 0.000 description 1
- 101100002068 Bacillus subtilis (strain 168) araR gene Proteins 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 108010064711 Homoserine dehydrogenase Proteins 0.000 description 1
- 101100276041 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) ctpD gene Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000010197 meta-analysis Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000006257 total synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 241001300301 uncultured bacterium Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/001—Amines; Imines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01018—Lysine decarboxylase (4.1.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/002—Vectors comprising a special translation-regulating system controllable or inducible
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (28)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710094155.XA CN108456687B (zh) | 2017-02-21 | 2017-02-21 | 基于赖氨酸浓度控制的重组表达质粒、转化子及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710094155.XA CN108456687B (zh) | 2017-02-21 | 2017-02-21 | 基于赖氨酸浓度控制的重组表达质粒、转化子及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108456687A CN108456687A (zh) | 2018-08-28 |
CN108456687B true CN108456687B (zh) | 2023-08-29 |
Family
ID=63221761
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710094155.XA Active CN108456687B (zh) | 2017-02-21 | 2017-02-21 | 基于赖氨酸浓度控制的重组表达质粒、转化子及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108456687B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111763699B (zh) * | 2019-04-02 | 2024-01-30 | 上海凯赛生物技术股份有限公司 | 用于发酵生产1,5-戊二胺的重组dna、菌株及其应用 |
CN110656119B (zh) * | 2019-10-09 | 2021-05-25 | 武汉博欧特生物科技有限公司 | 一种用于观察细菌蛋白定位的表达载体及其构建方法 |
CN114774510B (zh) * | 2022-04-12 | 2023-11-17 | 深圳大学 | 一种用于检测对硝基酚的试纸条及其制备方法与应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1433474A (zh) * | 2000-04-28 | 2003-07-30 | 华盛顿大学 | 调节性抗原传递系统(rads) |
CN103215286A (zh) * | 2012-11-12 | 2013-07-24 | 江南大学 | 用于发酵生产l-赖氨酸的重组dna、菌株及其应用 |
CN103409456A (zh) * | 2013-08-30 | 2013-11-27 | 温州医科大学 | 一种可诱导表达的疏螺旋体穿梭表达工具载体及构建方法和应用 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004027035A2 (en) * | 2002-09-20 | 2004-04-01 | Yale University | Riboswitches, methods for their use, and compositions for use with riboswitches. |
-
2017
- 2017-02-21 CN CN201710094155.XA patent/CN108456687B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1433474A (zh) * | 2000-04-28 | 2003-07-30 | 华盛顿大学 | 调节性抗原传递系统(rads) |
CN103215286A (zh) * | 2012-11-12 | 2013-07-24 | 江南大学 | 用于发酵生产l-赖氨酸的重组dna、菌株及其应用 |
CN103409456A (zh) * | 2013-08-30 | 2013-11-27 | 温州医科大学 | 一种可诱导表达的疏螺旋体穿梭表达工具载体及构建方法和应用 |
Non-Patent Citations (1)
Title |
---|
一种新发现的基因表达调控机制——核糖开关;赵谨等;《中国生物化学与分子生物学报》;20050430;第21卷(第2期);第151-157页 * |
Also Published As
Publication number | Publication date |
---|---|
CN108456687A (zh) | 2018-08-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110951741B (zh) | 一种基于CRISPR Cpf1的枯草芽孢杆菌多基因编辑和表达调控系统 | |
CN108587995B (zh) | 用于生产鼠李糖脂的细胞和方法 | |
CN108456687B (zh) | 基于赖氨酸浓度控制的重组表达质粒、转化子及其应用 | |
DK2663645T3 (da) | Gærstammer, der er modificeret til produktion af ethanol fra glycerol | |
DK2670846T3 (en) | METHODS FOR THE DEVELOPMENT OF TERPEN SYNTHASE VARIETIES | |
IL236992A (en) | Genetically modified cyanobacteria that produce ethanol | |
KR20130105649A (ko) | 피루베이트로부터 아세토락테이트로의 전환을 촉매작용시키는 폴리펩티드를 암호화하는 폴리뉴클레오티드의 통합 | |
CN104838016B (zh) | 用于识别相对于其野生型具有提高的特定代谢物细胞内浓度的细胞的方法 | |
KR20110076868A (ko) | 분리 조건 및/또는 안전한 작업 범위 하에서의 c5 탄화수소가 없는 이소프렌의 제조 방법 및 조성물 | |
KR20130045330A (ko) | 부탄올의 생성에서 추출 발효 전에 용해되지 않은 고형물을 제거하는 방법 및 시스템 | |
CN109825488A (zh) | 一种在大肠杆菌中进行木聚糖酶分泌表达的新方法 | |
CN112725256B (zh) | 重组大肠杆菌及利用重组大肠杆菌生物合成香叶木素的方法 | |
AU2016364229A1 (en) | Means and methods for preparing engineered proteins by genetic code expansion in insect cells | |
CN111566217A (zh) | 宿主细胞中风味化合物的产生 | |
CN110878322B (zh) | 一种用于肺炎克雷伯菌基因编辑的双质粒系统 | |
CN109136251A (zh) | 一种pCasPA/pACRISPR双质粒系统及其应用 | |
CN110964678B (zh) | 一种合成法尼烯的基因工程菌及其构建方法与应用 | |
CN110951770B (zh) | 一种简单快速高效的CRISPR/Cas9载体构建方法及应用 | |
AU2016289877A1 (en) | Method for gene optimization | |
CN116348106A (zh) | 卤代裸盖菇素衍生物及使用方法 | |
CN105018405A (zh) | 一株组成型表达基因工程菌及其生产l-丙氨酸的应用 | |
CN111607545A (zh) | 一种高产法尼烯的重组菌及其构建方法与应用 | |
KR102416059B1 (ko) | 에레모테시움의 발효를 통한 리보플라빈의 더 높은 생성을 위한 베타-산화 경로의 효소를 엔코딩하는 유전자 및 지방산 수송체 유전자의 과-발현 | |
TW202417610A (zh) | 用於在殺真菌素鏈黴菌之基因工程改造品系中增強生產持久殺菌素之組成物及方法 | |
CN113604500B (zh) | 一种甘蔗条纹花叶病毒全长cDNA侵染性克隆构建及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information |
Address after: 4 / F, building 5, No. 1690, Cailun Road, Shanghai Free Trade Zone Applicant after: CATHAY R&D CENTER Co.,Ltd. Applicant after: CATHAY INDUSTRIAL BIOTECH Ltd. Address before: 200120 Shanghai Zhangjiang High Tech Park of Pudong New Area Cailun Road No. 5 No. 1690 Applicant before: CATHAY R&D CENTER Co.,Ltd. Applicant before: CATHAY INDUSTRIAL BIOTECH Ltd. |
|
CB02 | Change of applicant information | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20191029 Address after: 4 / F, building 5, No. 1690, Cailun Road, Shanghai Free Trade Zone Applicant after: CATHAY R&D CENTER Co.,Ltd. Applicant after: CIBT USA Address before: 201203 floor 4, building 5, No. 1690, Cailun Road, Shanghai pilot Free Trade Zone Applicant before: CATHAY R&D CENTER Co.,Ltd. Applicant before: CATHAY INDUSTRIAL BIOTECH Ltd. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant |