CN108450327A - 一种抗性愈伤组织的筛选方法 - Google Patents
一种抗性愈伤组织的筛选方法 Download PDFInfo
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- CN108450327A CN108450327A CN201711499291.3A CN201711499291A CN108450327A CN 108450327 A CN108450327 A CN 108450327A CN 201711499291 A CN201711499291 A CN 201711499291A CN 108450327 A CN108450327 A CN 108450327A
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- callus
- resistant
- resistant calli
- screening
- rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
培养温度 | 18℃ | 20℃ | 22℃ | 24℃ | 26℃ | 28℃ |
愈伤诱导个数 | 346 | 547 | 514 | 520 | 524 | 508 |
愈伤诱导率 | 57.67% | 91.17% | 85.67% | 86.67% | 87.33% | 84.67% |
培养温度 | 18℃ | 20℃ | 22℃ | 24℃ | 26℃ | 28℃ |
愈伤诱导个数 | 336 | 447 | 464 | 512 | 504 | 498 |
愈伤诱导率 | 56% | 74.5% | 77.3% | 85.33% | 84% | 83% |
培养时间 | 12h | 24h | 36h | 48h |
接种愈伤组织块数 | 600 | 600 | 600 | 600 |
转化个数 | 225 | 254 | 237 | 235 |
转化率 | 37.5% | 42.3% | 39.5% | 39.2% |
培养时间 | 12h | 24h | 36h | 48h |
接种愈伤组织块数 | 600 | 600 | 600 | 600 |
转化个数 | 165 | 195 | 208 | 234 |
转化率 | 27.5% | 32.5% | 34.7% | 39% |
Claims (10)
Priority Applications (1)
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CN201711499291.3A CN108450327A (zh) | 2017-12-29 | 2017-12-29 | 一种抗性愈伤组织的筛选方法 |
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CN201711499291.3A CN108450327A (zh) | 2017-12-29 | 2017-12-29 | 一种抗性愈伤组织的筛选方法 |
Publications (1)
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CN108450327A true CN108450327A (zh) | 2018-08-28 |
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CN201711499291.3A Pending CN108450327A (zh) | 2017-12-29 | 2017-12-29 | 一种抗性愈伤组织的筛选方法 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140317782A1 (en) * | 2011-08-05 | 2014-10-23 | Shanghai Insititute For Biological Sciences, Chinse Academy Of Sciences | High temperature resistant plant gene and use thereof |
CN105695461A (zh) * | 2014-11-26 | 2016-06-22 | 中国科学院遗传与发育生物学研究所 | 一种小麦旗叶特异表达的启动子及其应用 |
CN106119276A (zh) * | 2016-06-29 | 2016-11-16 | 云南纳博生物科技有限公司 | 一种建立农杆菌介导灯盏花高效转染体系的方法 |
-
2017
- 2017-12-29 CN CN201711499291.3A patent/CN108450327A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140317782A1 (en) * | 2011-08-05 | 2014-10-23 | Shanghai Insititute For Biological Sciences, Chinse Academy Of Sciences | High temperature resistant plant gene and use thereof |
CN105695461A (zh) * | 2014-11-26 | 2016-06-22 | 中国科学院遗传与发育生物学研究所 | 一种小麦旗叶特异表达的启动子及其应用 |
CN106119276A (zh) * | 2016-06-29 | 2016-11-16 | 云南纳博生物科技有限公司 | 一种建立农杆菌介导灯盏花高效转染体系的方法 |
Non-Patent Citations (5)
Title |
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刘向东等: "《植物生殖生物学研究法》", 31 August 2012, 华南理工大学出版社 * |
李双成等: "提高农杆菌介导转化水稻效率的因素", 《中国水稻科学》 * |
李春龙: "《植物组织培养》", 31 July 2016, 西南交通大学出版社 * |
罗晓丽: "潮霉素作为选择抗生素在农杆菌介导转化中的应用", 《棉花学报》 * |
莽克强: "《农业生物工程》", 30 November 1998, 化学工业出版社 * |
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Address after: 266000 building 16, Jinshui Road, Licang District, Qingdao, Shandong, China 16 Applicant after: QINGDAO YUANCE GROUP Co.,Ltd. Address before: 266000 building 16, Jinshui Road, Licang District, Qingdao, Shandong, China 16 Applicant before: QINGDAO YUANCE BIOTECHNOLOGY Co.,Ltd. |
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Inventor after: Wang Kexiang Inventor after: Zhang Guodong Inventor after: Liu Jiayin Inventor after: Mi Tiezhu Inventor after: Xiuwangshan Inventor after: Sun Weihang Inventor before: Zhang Guodong Inventor before: Liu Jiayin Inventor before: Xiuwangshan Inventor before: Wang Kexiang Inventor before: Sun Weihang Inventor before: Mi Tiezhu |
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Application publication date: 20180828 |