CN108419590A - A method of it improving peach embryo and trains transplantation of seedlings survival rate - Google Patents
A method of it improving peach embryo and trains transplantation of seedlings survival rate Download PDFInfo
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- CN108419590A CN108419590A CN201810123746.XA CN201810123746A CN108419590A CN 108419590 A CN108419590 A CN 108419590A CN 201810123746 A CN201810123746 A CN 201810123746A CN 108419590 A CN108419590 A CN 108419590A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of methods that raising peach embryo trains transplantation of seedlings survival rate, include the following steps,（1）Arched shed is built, ensures that the air humidity in arched shed 95% or more, several planting grooves is arranged in arched shed, is filled out in planting groove and is covered with the suitable matrix of humidity；（2）It selects the embryo seedling with 3~5 true leaves as transplanting group embryo seedling, is transplanted；（3）The air humidity for keeping in arched shed 95% or more, after continuing 5~7 days, waters, and disposably irrigate when embryo seedling grows new root, sends out young leaves, waters again when matrix is dry；（4）It transplants seedlings：When selecting sunlight strength weak or cloudy, will normally grow, manage after transplanting 7~9 days and the peach embryo seedling that survives moves to seedling field.The present invention significantly improves peach embryo transplantation of seedlings survival rate to 99.3% or more, and process is easy, hence it is evident that reduces the workload transplanted seedlings, while being also obviously shortened the adaptation period that seedling grows in the environment of seedling field, makes seed stalwartness, well developed root system.
Description
Technical field
The present invention relates to peach seedling-raising technique fields, and in particular to a method of it improving peach embryo and trains transplantation of seedlings survival rate.
Background technology
Peach（Prunus Persica）It is perennial woody plant, as perennial fruit tree, peach breeding cycle length, workload
Greatly, and the development of tissue cultures accelerates peach breeding process.Precocious peach high financial profit, be breed breeding important directions it
One.When matching parent, precocious Peach cultivars fruit development period is shorter, embryo development is incomplete, often using conventional seed planting method
It cannot emerge or germination rate is low, therefore, hinder the selection and breeding of precocious Peach cultivars.The remote edge of peach can be overcome using peach embryo culture technique
The early stage abortion of embryo in crossbreeding, while can be with the excellent Early maturity kind of selection and breeding.Currently, China peach breeder is in morning
It does a lot of work in terms of ripe peach embryo culture, has cultivated many excellent early-maturing varieties.But the planting percent of embryo culture is also relatively low,
Only up to 70%, embryo trains the not perfect breeding efficiency for seriously constraining precocious peach of transplantation of seedlings technology.Therefore, morning is carried out in a deep going way
The embryo training transplantation of seedlings technical research of ripe peach is necessary.
The root system of most seedling is more flourishing after embryo culture, but a part of root quantity is few, growth potential is weak.Matrix
Type selects and soil humidity control is improper, often leads to the generation of root system pest and disease damage, and seedling death phenomenon often occurs after transplanting,
Survival rate is low when leading to be transplanted to crop field.
Therefore, it is current urgent problem to be solved to establish suitable transplantation technique system to improve peach seedling percent.
Invention content
The present invention provides a kind of method of raising peach embryo training transplantation of seedlings survival rate, it is intended to solve precocious peach embryo transplantation of seedlings and survive
The problem that rate is low, growth of seedling of the transplanting to crop field is unstable, seedling body is thin and weak.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
A kind of method of raising peach embryo training transplantation of seedlings survival rate is designed, is included the following steps,
（1）Matrix prepares：Matrix is allocated in peach embryo transplantation of seedlings the last week, is that matrix comes into full contact with moisture, and adjusts the base
The humidity of matter to matrix it is loose, hold it is agglomerating, unclamp and dissipate, have micro- tide sense but anhydrous exudation on hand；
（2）Embryo transplantation of seedlings place is arranged：Several planting grooves are set in the arched shed covered by White plastic film, by above-mentioned base
Matter, which is filled out, to be overlayed in planting groove, and controls the air humidity in arched shed 95% or more；
（3）Select seedling, hardening：Preceding 2~3 d of transplanting, selects the peach embryo seedling to grow fine to be placed on hardening in normal greenhouse, selection is grown
The peach embryo seedling of 3~5 true leaves is as transplanting group embryo seedling；
（4）Group embryo seedling transfer
A, slow seedling：By the planting groove in the transplanting group peach embryo transplantation of seedlings to arched shed, slow seedling is carried out after 1d, continues 5~7 d,
It during slow seedling, opens the ventilation opening of arched shed daily daytime, closes at night, keep ventilated in arched shed, and maintain in arched shed
Air humidity 95% or more；
B, it manages：After slow seedling, it is 95% or more to continue to keep air humidity in arched shed, is poured after embryo seedling sends out young leaves
Water, and disposably irrigate, next watering is carried out again when matrix is dry；
（4）Transplanted seedling field：When selecting sunlight strength weak or cloudy, by healthy and strong, the uniform peach of growing way after 7~9 d of above-mentioned transplanting
Embryo seedling moves to seedling field, normally grow, manages.
Preferably, the peach embryo seedling is the embryo seedling of precocious Peach cultivars of the maturity period before June 30.
Preferably, in step（1）In, the matrix is Pin Shi Tops of the import Denmark peat of pH 6.0.
Preferably, in step（1）In, use covered rearing with plastic film after the substrate allocation is good.
Preferably, in step（1）In, the arched shed size is 1.5 × 5m.
Preferably, in step（2）In, the air humidity in the arched shed is controlled using ultrasonic air humidifier.
Preferably, in step（4）In, the transplanting time of the tissue-cultured seedling is 6 to November every year.
Compared with prior art, the beneficial technical effect of the present invention lies in：
1, the application overcomes this field conventional technical means, not directly waters after embryo transplantation of seedlings but after embryo transplantation of seedlings
Slow seedling is first carried out, moisture needed for plant growth is maintained by the air humidity controlled in arched shed, and make up plant because of transpiration
And lost moisture, this not only avoids the rotten and hardening slow seedlings of root system of plant and rhizome part caused by excessive watering
When burn the generation of leaf, yellow leaf problem, additionally it is possible to the effective gas permeability for keeping matrix fundamentally avoids plant due to rotten
Dead phenomenon occurs, and significantly shortens adaptation period of the seedling to growing environment, makes seed robust growth, well developed root system.
2, the present invention transplants the generation that peach embryo trains the humidity reduction bad root phenomenon of seedling matrix by control, while increasing plant
The humidity of strain upper air makes up the lost moisture of transpiration, and pole considerably improves the survival rate of peach embryo training seedling, simultaneously
The survival rate transplanted seedlings of seedling is improved to 99.3% or more.
3, matrix used, can be directly practical without carrying out disinfection in the present invention, greatly reduces workload when transplanting seedlings；It adopts
With ultrasonic air humidifier, unartificial lasting sprinkling only need to pre-set ultrasonic wave to ensure the air humidity needed for slow seedling
Air humidifier can effectively ensure that humidification range is uniform, effect is good, while the labour for also significantly reducing worker is strong
Degree.
Specific implementation mode
Illustrate the specific implementation mode of the present invention with reference to specific embodiment, but following embodiment is used only in detail
Illustrate the present invention, does not limit the scope of the invention in any way.In the examples below involved instrument and equipment for example without
It illustrates, is routine instrument device；Involved management method is conventional method unless otherwise instructed.
Embodiment 1：A method of it improving peach embryo and trains transplantation of seedlings survival rate,
Test area：Zhengzhou City Henan Province peach breeding nursery；
Time：Transplanting time of the present invention is suitable for annual October to December.The time that the present embodiment is specifically tested is
In October, 2017 to December；
Peach embryo kind：The present invention selects precocious Peach cultivars of the maturity period before June 30, using the embryo seedling of the kind as this hair
Bright test material.In specifically being used in the present embodiment for oily No. 13 peach embryo seedlings.
1, this method the specific steps are：
（1）Matrix prepares and selection：With Pin Shi Tops of the Denmark peat of import（The Sphagnum Peat）For matrix, pH
6.0.Allocated with tap water in early October, 2017, matrix made to be come into full contact with moisture, adjust soil humidity to matrix it is loose,
Agglomerating, release is held to dissipate, have micro- tide sense but anhydrous exudation on hand.Covered rearing with plastic film moisturizing is used after substrate preparation is good,
And matrix and moisture is made to come into full contact with, promote that matrix is loose, humidity is uniform, good air permeability uses after a week.
（2）Embryo transplantation of seedlings place is arranged：After a week, the arched shed that 1.5 × 5m is built in the Experimental Area of above-mentioned selection, with
Just it embryo seedling energy and is transplanted into arched shed, then uses white clear covered rearing with plastic film, and ultrasonic wave air is set in arched shed and is added
Wet device, to ensure the air humidity in arched shed 95% or more.Several planting grooves are set in arched shed, are filled up in planting groove
State the suitable matrix of prepared humidity.Wherein, used ultrasonic air humidifier model connection reputation H-010, in being purchased from
Shuai Qi Electrical Appliances Co., Ltd of mountain city.
（3）Hardening selects seedling：It selects the peach embryo seedling to grow fine to be placed in normal greenhouse, carries out embryo seedling culture, select embryo seedling
The peach embryo seedling of 3~5 true leaves is grown as transplanting group embryo seedling；
（4）Group embryo seedling transfer：By in above-mentioned group of embryo transplantation of seedlings to the planting groove of arched shed, transplanting carries out slow seedling after 1 day, and slow seedling is lasting
Time is 5~7 d.It during slow seedling, opens the ventilation opening of arched shed daily daytime, closes at night, keep ventilated in arched shed, and
Increase the fog-supplying amount of ultrasonic air humidifier simultaneously, ensures the air humidity in arched shed 95% or more；
After the completion of slow seedling, keeps being covered with water mist on new transplanting peach embryo seedling leaf, embryos seedling is waited to grow new root in planting groove, send out
It waters after young leaves, and disposably irrigates, next watering is carried out again when matrix is dry.
（5）Transplanted seedling field：When selecting sunlight strength weak or cloudy, it will grow after in above-mentioned transplanting to arched shed 7~9
Healthy and strong, the uniform peach embryo seedling replanting of growing way carries out conventional growth, management to seedling field.It in addition to this, can be as needed
Artificially feed appropriate is made to plant.
2, growth of seedling condition survey in seedling field
Selection grows each 120 plants of the healthy and strong embryo seedling of 3~5 true leaves, as test group 1 to 3,3 repetitions of every group of setting respectively；Together
When choose identical with 1 to 3 size of test group, growing way 360 plants of peach embryo seedlings, accordingly as a control group 1 to 3, every group same
Three repetitions are set.Wherein, the embryo seedling of test group 1 to 3 is transplanted according to the method for embodiment 1, and control group 1 to 3 is according to conventional side
Method is transplanted, and peach embryo transplantation of seedlings to Miao Tianhou is all made of conventional, identical way to manage.Then it conventionally unites respectively
The transplanting survival rate of each group plant, situation of taking root, disease incidence, statistical result after meter transplanting to seedling field 15d, 30d, 60d
According to every group of mean value calculation, design parameter is referring to table 1.
Data in table 1 are compared and analyzed it is found that using method recorded in the present invention by peach embryo transplantation of seedlings to seedling
After Tanaka carries out field planting growth, seedling growth is consistent, seed is healthy and strong, relative to control group, either transplant 15 days, 30 days still
60 days, the root system of test group peach seedling was all relatively abundant, more sturdy, and aggregate performance is well developed root system, and transplanting survival rate relatively compares
Group is greatly improved, and overall effect is substantially better than control group.
The present invention is described in detail above in conjunction with specific embodiment, still, person of ordinary skill in the field
It is understood that without departing from the purpose of the present invention, can also become to each design parameter in above-described embodiment
More, multiple specific embodiments are formed, is the common variation range of the present invention, is no longer described in detail one by one herein.
Claims (7)
1. a kind of method improving peach embryo training transplantation of seedlings survival rate, which is characterized in that include the following steps,
（1）Matrix prepares：Peach embryo transplantation of seedlings the last week allocate matrix, so that matrix is come into full contact with moisture, the matrix it is wet
Degree allotment to matrix it is loose, hold it is agglomerating, unclamp and dissipate, have micro- tide sense but anhydrous exudation on hand；
（2）Embryo transplantation of seedlings place is arranged：Several planting grooves are set in the arched shed covered by White plastic film, by above-mentioned base
Matter, which is filled out, to be overlayed in planting groove, and controls the air humidity in arched shed 95% or more；
（3）Select seedling, hardening：Preceding 2~3 d of transplanting, selects the peach embryo seedling to grow fine to be placed on hardening in normal greenhouse, selection is grown
The peach embryo seedling of 3~5 true leaves is as transplanting group embryo seedling；
（4）Group embryo seedling transfer
A, slow seedling：By the planting groove in the transplanting group peach embryo transplantation of seedlings to arched shed, slow seedling is carried out after 1d, continues 5~7 d,
It during slow seedling, opens the ventilation opening of arched shed daily daytime, closes at night, keep ventilated in arched shed, and maintain in arched shed
Air humidity 95% or more；
B, it manages：After slow seedling, the air humidity in arched shed is continued to 95% or more, is carried out after embryo seedling sends out young leaves
Watering, and disposably irrigate, next watering is carried out again when matrix is dry；
（4）Transplanted seedling field：When selecting sunlight strength weak or cloudy, by healthy and strong, the uniform peach of growing way after 7~9 d of above-mentioned transplanting
Embryo seedling moves to seedling field, normally grow, manages.
2. the method according to claim 1 for improving peach embryo training transplantation of seedlings survival rate, which is characterized in that the peach embryo seedling is
Precocious peach embryo seedling of the maturity period before June 30.
3. the method according to claim 1 for improving peach embryo training transplantation of seedlings survival rate, which is characterized in that in step（1）In,
The covered rearing with plastic film moisturizing of deployed matrix.
4. the method according to claim 1 for improving peach embryo training transplantation of seedlings survival rate, which is characterized in that in step（1）
In, the matrix is Pin Shi Tops of the import Denmark peat of pH 6.0.
5. the method according to claim 1 for improving peach embryo training transplantation of seedlings survival rate, which is characterized in that in step（1）In,
The arched shed size is 1.5 × 5m.
6. the method according to claim 1 for improving peach embryo training transplantation of seedlings survival rate, which is characterized in that in step（2）In,
Air humidity in the arched shed is controlled using ultrasonic air humidifier.
7. the method according to claim 1 for improving peach embryo training transplantation of seedlings survival rate, which is characterized in that in step（4）In,
The transplanting time of the tissue-cultured seedling is 6 to November every year.
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| CN201810123746.XA CN108419590B (en) | 2018-02-07 | 2018-02-07 | Method for improving transplanting survival rate of peach embryo cultured seedlings |
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| CN201810123746.XA CN108419590B (en) | 2018-02-07 | 2018-02-07 | Method for improving transplanting survival rate of peach embryo cultured seedlings |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0248131A1 (en) * | 1985-06-03 | 1987-12-09 | Plant Research Laboratories | A method for acclimatizing and propagating plant tissue culture shoots |
| CN1436448A (en) * | 2003-02-26 | 2003-08-20 | 陕西师范大学 | Fast reproduction process of Ma Hali cherry stock |
| CN102763599A (en) * | 2012-08-20 | 2012-11-07 | 甘肃省科学院生物研究所 | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method |
| CN107409990A (en) * | 2017-08-11 | 2017-12-01 | 上海市农业科学院 | A kind of method of peach embryo training seedling Field planting then |
-
2018
- 2018-02-07 CN CN201810123746.XA patent/CN108419590B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0248131A1 (en) * | 1985-06-03 | 1987-12-09 | Plant Research Laboratories | A method for acclimatizing and propagating plant tissue culture shoots |
| CN1436448A (en) * | 2003-02-26 | 2003-08-20 | 陕西师范大学 | Fast reproduction process of Ma Hali cherry stock |
| CN102763599A (en) * | 2012-08-20 | 2012-11-07 | 甘肃省科学院生物研究所 | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method |
| CN107409990A (en) * | 2017-08-11 | 2017-12-01 | 上海市农业科学院 | A kind of method of peach embryo training seedling Field planting then |
Non-Patent Citations (1)
| Title |
|---|
| 王玉安: "桃胚培养及种质创新研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
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