CN108341714A - Zymolyte and its preparation method and application - Google Patents
Zymolyte and its preparation method and application Download PDFInfo
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- CN108341714A CN108341714A CN201810262040.1A CN201810262040A CN108341714A CN 108341714 A CN108341714 A CN 108341714A CN 201810262040 A CN201810262040 A CN 201810262040A CN 108341714 A CN108341714 A CN 108341714A
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- zymolyte
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The present invention relates to fertilizer fields, more particularly to zymolyte and its preparation method and application.Zymolyte provided by the invention can induce plant and generates resistance, and the immune system of activated plant itself resists infecting for disease, reduces the use of chemical pesticide, and then avoid the problem of chemical pesticide is brought.Water soluble fertilizer added with zymolyte provided by the invention or auxiliary agent not only has the function of that supplying plant nutrient also has effects that induce plant stress-resistance, realizes medicine fertilizer economic benefits and social benefits.
Description
Technical field
The present invention relates to fertilizer fields, more particularly to zymolyte and its preparation method and application.
Background technology
Fertilizer is the necessity of agricultural production, but since the use of chemical fertilizer brings many pollutions to our life
Problem, for example, the eutrophication in rivers and creeks lake, soil are contaminated, soil physical properties deterioration, food and feed and drinking water
Middle toxic component increases, NO_x in Air Environment content increases etc..From 1977~2015 years China's fertilizer amounts and grain yield
Data statistics from the point of view of, the yield increasing effect of chemical fertilizer is slowly weakening.In other words, even if we using chemical fertilizer more again to grain
Food volume increase role is also very faint, that is to say, that the amount of application of the current chemical fertilizer in China is excessive.Chemical fertilizer poor efficiency is
The main reason for causing chemical fertilizer to be excessively used.Our living necessities grains, fertilizer application is necessary, but before being to continue with
The problems such as tradition must continue to bring body eutrophication, soil environment to deteriorate using chemical fertilizer mode, it is conflicting in the two but
It must and deposit instantly again, it would be desirable to change traditional fertilizer technology, need proper use of chemical fertilizer, that is, improve fertilizer profit
With rate, the environmental problem brought is solved, the fertilizer nutrient being manured into soil is made to be absorbed and used by plants completely as possible.
Currently, solution fertilizer nutrient utilization is low there are mainly two types of approach, an approach is to improve fertilizer practice, surveys soil
Formula technique is the fertilizer practice that can improve utilization rate of fertilizer that the Ministry of Agriculture carries out, compared with common fertilising, Ke Yiyou
Effect ground accomplishes that soil lacks what what mends, and improves fertilizer utilization efficiency to a certain extent；In addition to this, water-fertilizer integral skill
Art, which is also one of Ministry of Agriculture's implementation, to be had the new fertilizer practice of agricultural to combine together with fertilising is irrigated compared with commonly fertilising
The advantages of saving water-saving fertilizer, precision fertilizing, and then fertilizer utilization efficiency can be improved.Another solution route is to improve fertilizer skill
Art, that is, initiative can improve the new-type fertilizer of utilization rate of fertilizer, and new-type fertilizer can be regarded as supporting can provide plant mineral
Based on the substance divided, by physics, chemistry or biological effect, the one kind for making the trophic function of soil and crop get a promotion
Fertilizer, primary efficacy are to improve nutrien utilization condition or raising plant recovery of nutrient.
Fertilizer supply plant nutrient promotes growth, pesticide control disease to ensure growth.Plant disease is to agricultural production
Have a great impact, Severe Reduction will be caused when disease large-scale outbreak.Chemical pesticide has speed to crop disease control
The features such as imitating, is efficient, prevention is thorough can be effectively protected crops health, reduce agricultural losses caused by due to disease.Change
Although a large amount of uses for medicine of learning to farm have efficiently controlled plant disease, while also disease pest being made to develop immunity to drugs, germ variation,
Virtually increase disease control work difficulty, and the use of chemical pesticide also caused to ecological environment and food security it is huge
Big threat, directly affects human health.Efficient sterilizing deinsectization, novel agrochemical environmental-friendly, harmless to humans are the following agricultures
The ideal plant protection product of industry.More and more research shows that the ego defense of the owned complete set of plant protects system,
It is equivalent to the immune system of human body, invading for disease can be resisted with the immune system of activated plant itself by mode appropriate
Dye, thus without using chemical pesticide, also avoids the negative effect that chemical pesticide is brought.
Sugar is widespread in nature as one of three categories biomolecule, is a kind of very important in plant
Organic compound acts not only as substrate of respiration and provides energy for the growth and development of plant, may also participate in generation in plant
It thanks to activity, other substances is synthesized again by metabolic pathway as intermediate.In recent years more and more research shows that sugar and egg
White matter, nucleic acid equally have semiotic function, and the signal network system of plant complexity is formed together with other signaling molecules.Thus
As it can be seen that sugar plays a crucial role growth and development of plants and gene expression.Sugar is in food, health care, medicine, feed etc.
There is more application in field, but agriculturally applies less.Chitosan class, seaweed acids fertilizer are glucides in agricultural
The typical case of upper application, this two substance are mainly derived from shrimp and crab shells and marine algae, and extraction cost is higher, and former material
Material is by territory restriction.
Therefore it provides a kind of efficient, safe, at low cost fertilizer has important practical significance.
Invention content
In view of this, a kind of zymolyte of present invention offer and its preparation method and application.Zymolyte energy provided by the invention
The plant is induced to generate resistance, the immune system of activated plant itself resists infecting for disease, reduces making for chemical pesticide
With, and then avoid the problem of chemical pesticide is brought.Water soluble fertilizer added with zymolyte provided by the invention or auxiliary agent not only has
Playing the role of supply plant nutrient also has effects that induce plant stress-resistance, realizes medicine fertilizer economic benefits and social benefits.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides a kind of preparation methods of zymolyte, are 4~7 in pH value using plant as raw material, temperature 30
It is digested under conditions of~60 DEG C.
In some specific embodiments of the present invention, the enzyme is cellulase, zytase, mannonase half
Mixture more than one or both of newborn mannase or pectase.
In some specific embodiments of the present invention, the enzyme activity of the cellulase is 2000~100000u/g；Wood
The enzyme activity of dextranase is 1000~42000u/g；The enzyme activity of mannase is 2000~50000u/g；Galactomannans enzyme
Enzyme activity be 1000~35000u/g；The enzyme activity of pectase is 1000~100000u/g.
In some specific embodiments of the present invention, the plant is one kind in konjaku, agricultural crop straw or trees
Or both more than mixture.
In some specific embodiments of the present invention, the time of the enzymolysis is 10~20h.
In some specific embodiments of the present invention, the pH value is 5.
In some specific embodiments of the present invention, the temperature is 50 DEG C.
The present invention also provides zymolytes made from the preparation method.
The present invention some specific embodiments in, zymolyte of the present invention include glucose, galactolipin,
Mixture more than one or both of mannose, Portugal's mannose, fructose or xylose.
In some specific embodiments of the present invention, zymolyte of the present invention includes glucose and mannose,
The mass ratio of glucan and mannose is 2:3.
In other specific embodiments of the present invention, zymolyte of the present invention includes Portugal's mannose and half
The mass ratio of lactose, Portugal's mannose and galactolipin is 1:1.
In some specific embodiments of the present invention, zymolyte of the present invention further includes protein, polypeptide, ammonia
It is one or more in base acid.
In other specific embodiments of the present invention, the matter of sugar, protein, polypeptide, amino acid in the zymolyte
Measuring ratio is：(30~75)：(1~15)：(1~10)：(1~15).
The present invention also provides the zymolyte plant supply nutrition, it is disease-resistant or degeneration-resistant in application.
The present invention also provides the zymolytes to prepare fertilizer synergist, soil conditioner, microorganism growth promotion
Application in agent, seed soaking agent, biological pesticide, Special Fertilizers for Various Crops.
The present invention also provides a kind of auxiliary agents, including zymolyte of the present invention.
In some specific embodiments of the present invention, the auxiliary agent is in terms of mass parts, including 30~60 parts of present invention carry
The zymolyte of confession, 20~50 parts of organic active substances, trace element, 1~10 part of plant growth regulator in 1~10 part.
Wherein, zymolyte provided by the invention：Including glucose, galactolipin, mannose, Portugal's mannose, fructose, xylose
Deng it is one or more.Wherein, glucose, galactolipin, mannose, Portugal's mannose, fructose, xylose mass ratio be (10~
25)：(1~10)：(3~35)：(1~10)：(1~5)：(1~25).
Organic active substance：Including one in humic acid, humic acid potassium, fulvic acid, potassium fulvate, polymeric amino acid etc.
Kind is a variety of.
Middle trace element：One kind or more including zinc sulfate, magnesium sulfate, ammonium molybdate, ferric sulfate, borax, manganese sulfate etc.
Kind.
Plant growth regulator：Including compound sodium nitrophenolate, heteroauxin, methyl α-naphthyl acetate, brassin lactones, diethyl aminoethyl hexanoate, 30
Alkanol etc. it is one or more.
In some specific embodiments of the present invention, auxiliary agent provided by the invention is in terms of mass percentage, including such as
Lower component：
Zymolyte：Ligoglucoside 20%, manna oligosacchride 30%
Organic active substance：Glutamic acid 23%, potassium fulvate 15%
Middle trace element：Magnesium sulfate 3%, borax 2%
Plant growth regulator：Brassin lactones 3%, diethyl aminoethyl hexanoate 4%.
The present invention other specific embodiments in, auxiliary agent provided by the invention in terms of mass percentage, including
Following component：
Zymolyte：Mannan oligosaccharide 25%, galactooligosacchari(es 25%
Organic active substance：Fulvic acid 20%, poly-aspartate 20%
Middle trace element：Magnesium sulfate 2%, zinc sulfate 2%
Plant growth regulator：Triacontanol 2%, diethyl aminoethyl hexanoate 4%.
The present invention also provides acceptable in a kind of fertilizer, including zymolyte of the present invention or auxiliary agent and fertilizer
Auxiliary material.
In some specific embodiments of the present invention, the fertilizer is Water soluble fertilizer.
In some specific embodiments of the present invention, the auxiliary agent of 5% (w/w) is contained in the Water soluble fertilizer.
The present invention also provides acceptable auxiliary materials in a kind of pesticide, including zymolyte of the present invention and pesticide.This
The zymolyte that invention provides can induce plant and generates resistance, and the immune system of activated plant itself resists infecting for disease,
The use of chemical pesticide is reduced, and then avoids the problem of chemical pesticide is brought.Added with zymolyte provided by the invention or help
The Water soluble fertilizer of agent not only has the function of that supplying plant nutrient also has effects that induce plant stress-resistance, realizes medicine fertilizer economic benefits and social benefits.
Raw material sources needed for the present invention are extensive, without geographical restrictions, are easily obtained, cheap, substantially reduce product at
This.
The present invention main component --- zymolyte extract through advanced biochemical process, natural environmental-protective, rich in plant
A variety of nutriments needed for growth, are easily absorbed by crops utilization.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will to embodiment or
Attached drawing needed to be used in the description of the prior art is briefly described.
Fig. 1 shows the result of the thin-layer chromatography qualitative analysis of enzymolysis product；
Fig. 2 shows that anthrone colorimetry measures the standard curve of sugared content.
Specific implementation mode
The invention discloses a kind of zymolyte and its preparation method and application, those skilled in the art can use for reference herein
Content is suitably modified technological parameter realization.In particular, it should be pointed out that all similar substitutions and modifications are to art technology
It is it will be apparent that they are considered as being included in the present invention for personnel.The method of the present invention and application are by preferable
Embodiment is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to side as described herein
Method and application are modified or suitably change and combine, to realize and apply the technology of the present invention.
The present invention obtains sugar by enzymatic hydrolysis and fermentation technique, and raw materials used plant origin is extensive, and enzyme used includes cellulose
Enzyme, mannonase mannosidase etc..Between pH4~7, under 30~60 DEG C of temperature conditions, the product digested contains
Sugar amount is higher.Using silica gel plate as carrier, the mixed liquor of n-butanol, ethyl acetate and water is as solvent to the sugar after enzymolysis
It is detached, thin-layer chromatography qualitative analysis, analysis result display extraction is carried out by color developing agent of the mixed liquor of aniline-diphenylamines
Sugared ingredient include mannose, glucose, fructose, xylose, galactolipin and oligosaccharide.Product after enzymolysis is in addition to sugar, also egg
White matter, amino acid, lignin, cellulose, middle trace element etc., through further cleaning, filtering, spray drying obtains water solubility
Preferably enzymolysis sugar.
Method provided by the invention is using plant as raw material, the wherein preferred tropical coast plant of plant, by tropical coast plant
After dust is removed in cleaning, natural conditions drying is dried after pulverization process.A certain amount of mannase and fiber is added
Plain enzyme solution, set temperature and rotating speed react in temperature control shaking table.Temperature is very big on chemical reaction influence, anti-for enzymatic
It answers, is less than a certain temperature, reaction possibly can not carry out, and be higher than a certain temperature, and enzyme may inactivate.Enzymolysis time is in practical life
It is critically important in production, contribute to rational allocation production task, in different enzymolysis time points, measures sugared content in enzymolysis liquid.Certain
It is digested under temperature condition after a certain period of time, enzymolysis liquid boiling water bath is taken to terminate reaction, measured in enzymolysis liquid with anthrone colorimetry
Sugared content probes into the efficiency of enzyme digestion reaction under different temperatures different time.
Temperature is very big on chemical reaction influence, for enzymic catalytic reaction, is less than a certain temperature, and reaction possibly can not carry out,
Higher than a certain temperature, enzyme may inactivate.For digesting konjaku extraction sugar, explored between 30-60 DEG C in the enzymolysis process
Best enzymatic reaction temperature, concrete outcome are shown in Table 1.Enzyme digestion reaction efficiency when under different temperatures 50 DEG C known to sugared content
It is higher.
Enzymolysis time is critically important in actual production, contributes to rational allocation production task, in different enzymolysis time points,
Sugared content in enzymolysis liquid is measured, as shown in table 2, when enzymolysis time reaches 20h, sugared content reaches higher level in enzymolysis liquid, when
Between extending for improving sugared content without too big effect, consider, enzymolysis suitable time is 20h.
Removal of impurities and filtration step：In plate and frame type filter-press feed slurry (liquor) inlet except enzymolysis liquid is introduced, according to 3kg/m3Dosage add
Enter diatomite as filter aid, be filtered using sheet frame, collects filtrate.The filtrate being collected into is concentrated into sugar content 30-
40%, fine-powdered sugar is dried into using spray drying.
To after spray-dried product carry out dissolubility test, it is found that its is soluble easily in water, solubility is good,
99% or more.
Zymolyte：It extracts and obtains from plant through enzymolysis process.Plant can be effectively induced to generate resistance, enhancing is planted
Nutrient is absorbed and utilized in object, and carbon source is provided for plant.The zymolyte said herein refers to filtrate after removal filtration through spraying
The powder sugar obtained after drying.The sugar extracted from plant through enzymolysis process can effectively enhance absorption of the plant to nutrient
It utilizes, and plant is induced to generate resistance.
The zymolyte extracted by biochemical fermentation technique is actually used in Water soluble fertilizer by the present invention, based on oligosaccharides,
It compounds with organic active substance, plant growth regulator, and is especially added with middle trace element, main function is that activation is planted
Object immune system, induction plant generate defense response, enhance degeneration-resistant, disease resistance, promote absorption of the plant to fertilizer nutrient, carry
High utilization rate of fertilizer.
Majority fertilizer product can only provide nutrition to plant at present.The present invention is mainly the enzymolysis for obtaining enzymatic hydrolysis and fermentation
Object is added in Water soluble fertilizer, by nutrition supply and disease-resistant degeneration-resistant function integrated optimization, enriches the work(of existing water-soluble fertilizer product
Can, the use of chemical pesticide can be efficiently reduced, chemical pesticide is avoided to be asked using environmental pollution, the food security etc. brought
Topic.
Most Water soluble fertilizer adding ingredients are obtained by chemical methodes such as soda acid processing, chemical syntheses, lack physiological activity object
Matter.The main component of the present invention derives from plant cell, is extracted through advanced biochemical process, natural environmental-protective, is rich in plant
A variety of nutriments needed for growth, are easily absorbed by crops utilization.
Most Water soluble fertilizer adding ingredients are of high cost, are not easy to obtain, raw material is by territory restriction.Raw material sources needed for the present invention
Extensively, without geographical restrictions, it is easily obtained, it is cheap, reduce product cost.
Product of the present invention is added in common Water soluble fertilizer by 1%~10% ratio uniform up to zymolyte Water soluble fertilizer, work
Skill is simple and convenient, without especially increasing cost of equipment.
The present invention obtains zymolyte by enzymatic isolation method, and raw material sources used are extensive, such as konjaku, agricultural crop straw, tree
Wood etc..Enzyme used includes a variety of enzymes, such as cellulase, zytase, mannonase pectase etc., is extracted after enzymolysis
The zymolyte ingredient gone out includes that gala Portugal mannose, mannose, glucan, galactolipin, Portugal's mannose, fructose, xylose etc. are a variety of
It is one or more in sugar.Preferably, in pH5.5, under the conditions of 50 DEG C, the product sugar content highest that digests.
The component of the present invention mainly has：30~60 parts of zymolytes, 20~50 parts of organic active substances are micro in 1~10 part
The above component is uniformly mixed up to product of the present invention by element, 1~10 part of plant growth regulator.
Product of the present invention is uniformly added in common Water soluble fertilizer by 5% up to zymolyte Water soluble fertilizer.
The feature of environmental protection：The present invention is mainly added to zymolyte as auxiliary agent main component in Water soluble fertilizer, by nutrition supply
With disease-resistant degeneration-resistant function integrated optimization, the function of existing water-soluble fertilizer product is enriched, making for chemical pesticide can be efficiently reduced
With, avoid chemical pesticide using bring environmental pollution, food security the problems such as.
Zymolyte provided by the invention can also be applied to fertilizer synergist, soil conditioner, microorganism growth promotion
Agent, seed soaking agent, biological pesticide, Special Fertilizers for Various Crops etc..
There are chitosan oligosaccharide, alga oligosaccharides of similar effect etc. to derive from aquatic animals and plants, acquisition, production cost are high, and have
There is seasonal (seaweed, kelp mature period property), the oligosaccharide source that the present invention uses is derived from a wealth of sources in land plant, and price is low
It is honest and clean, it can more preferably carry out industrialization.
Having the chitosan oligosaccharide of similar effect, alga oligosaccharides extraction process, some use soda acid violence edman degradation Edman, have pollution to remain
(risk), the present invention use enzymatic isolation method, main component to derive from plant cell, are extracted through advanced biochemical fermentation technique,
Natural environmental-protective is easily absorbed by crops utilization rich in a variety of nutriments needed for plant growth.Process conditions are mildly environmentally friendly, nothing
Harmful side product.
It is raw materials used in zymolyte provided by the invention and its preparation method and application to be available on the market with reagent.
With reference to embodiment, the present invention is further explained：
The preparation of 1 zymolyte of embodiment
It is 5.5 in pH value using konjaku as raw material, under conditions of temperature is 45 DEG C, through the fiber that enzyme activity is 100000u/g
Plain enzyme, 20000u/g zytases, 50000u/g mannonases 20000u/g galactomannans enzyme and 100000u/g fruits
Glue enzyme digests 15h.
In zymolyte obtained, sugared content is 75% (w/w)；The content of protein is 15% (w/w)；Polypeptide contains
Amount is 3% (w/w)；The content of amino acid is 1% (w/w).
Gained sugar includes glucose 25% (w/w), galactolipin 2% (w/w), mannose 35% (w/w), Portugal's mannose
10% (w/w), fructose 2% (w/w), xylose 1% (w/w).
The preparation of 2 zymolyte of embodiment
It is 7 in pH value using agricultural crop straw as raw material, under conditions of temperature is 30 DEG C, through the fibre that enzyme activity is 50000u/g
The plain enzyme of dimension, 42000u/g zytases, 30000u/g mannonases 1000u/g galactomannans enzyme and 1000u/g fruits
Glue enzyme digests 10h.
In zymolyte obtained, sugared content is 60% (w/w)；The content of protein is 1% (w/w)；The content of polypeptide
For 10% (w/w)；The content of amino acid is 5% (w/w).
Gained sugar includes glucose 15% (w/w), galactolipin 10% (w/w), mannose 3% (w/w), Portugal's mannose 2%
(w/w), fructose 5% (w/w), xylose 25% (w/w).
The preparation of 3 zymolyte of embodiment
Using trees as raw material, pH value be 4, temperature be 60 DEG C under conditions of, through enzyme activity be 2000u/g cellulase,
1000u/g zytases, 2000u/g mannonases 35000u/g galactomannans enzyme and 30000u/g pectinase enzymatic hydrolysis
20h。
In zymolyte obtained, sugared content is 30% (w/w)；The content of protein is 4% (w/w)；The content of polypeptide
For 1% (w/w)；The content of amino acid is 15% (w/w).
Gained sugar includes glucose 10% (w/w), galactolipin 2% (w/w), mannose 10% (w/w), Portugal's mannose 1%
(w/w), fructose 1% (w/w), xylose 6% (w/w).
The preparation of 4~10 auxiliary agent of embodiment
Embodiment 11
Detect the pot test effect of zymolyte provided by the invention or the auxiliary agent containing the zymolyte.With provided by the invention
Zymolyte or auxiliary agent containing the zymolyte are experimental group, and clear water is blank control group, and market similar product compares, small
The effect of zymolyte or the auxiliary agent containing the zymolyte is verified on Chinese cabbage, identical base fertilizer is all applied in all processing.
Influence of the table 1 to chlorophyll content and biomass dry weight
Table 2 fights the influence of peroxidase activity and cell membrane lipid Peroxidation Product
By spray different disposal compare pakchoi items growth indexes, find application zymolyte provided by the invention and
The pakchoi growing way of auxiliary agent containing the zymolyte is better than control group.Pass through biomass, chlorophyll content, antioxidant enzyme
Active (superoxide dismutase SOD, peroxidase POD, cat catalase), cell membrane lipid Peroxidation Product MDA) contain
The indexs such as amount carry out the difference of specific contrast experiment group and control group.General plant is saline and alkaline such as high temperature under adverse environmental factor, and
Membrane lipid peroxidation is just will produce under the adverse environmental factors such as strong light, mda content is the embodiment of plant cell film quality extent of peroxidation,
Mda content is high, illustrates that plant cell film quality extent of peroxidation is high, the injury that cell membrane is subject to is serious.It can from these indexs
To detect zymolyte provided by the invention and the effect of the auxiliary agent induction plant generation resistance containing the zymolyte.
Experimental group and the comparison of control group biomass and chlorophyll content are it is found that using enzymolysis provided by the invention from table 1
The pakchoi growing way of object and auxiliary agent containing the zymolyte is better than control group, and each experimental group biomass and chlorophyll content ratio are empty
23.8~41.2% and 23.3~43.3% are respectively increased in white control group.The value of SOD, POD, CAT are all to apply this hair in table 2
The processing group of the zymolyte of bright offer and auxiliary agent containing the zymolyte is compared with control group height, but the value of MDA becomes in opposite
Gesture illustrates that zymolyte provided by the invention and the auxiliary agent containing the zymolyte can excite plant anti-oxidation enzymatic activity, removes and plant
The MDA that excessive active oxygen radical and peroxidation of membrane lipids generate in object, maintains the metabolic balance of active oxygen, most
Play the role of protecting membrane structure eventually, further relating to zymolyte provided by the invention and the auxiliary agent containing the zymolyte can lure
It leads plant and generates resistance, slow down the stress from outside that plant is subject to.
Embodiment 12
By zymolyte provided by the invention and the auxiliary agent containing the zymolyte, (1~embodiment of embodiment 10 is obtained to produce
Product) it is added in Water soluble fertilizer in 5% ratio and obtains Water soluble fertilizer provided by the invention.The Water soluble fertilizer of the present invention is used for cell water
Rice is tested, and 12 processing of experimental design, 12 processing apply conventional composite fertilizer (N-P-K by 40kg/ mus of bases：25-8-12),
It is handled differently respectively in jointing stage, booting later stage and pustulation period top dressing, specially (1) not top dressing, (2) impose routine
Water soluble fertilizer (N-P-K:28-12-12), (3~12) impose the nutrients such as equivalent zymolyte fertilizer (N-P-K:28-12-12 is routinely water-soluble
Fertilizer+zymolyte fertilizer synergist).10 meters of zone leader, 4 meters wide, area 40m2, surrounding, which is set, protects row.
In each processing group, the rice leaf color of zymolyte Water soluble fertilizer foliage applying processing is dark green, and blade is straight and upright, and growth is neat,
Field growing way is best.As shown in Table 3, rice number of productive ear, mass of 1000 kernel and rice production can be improved by imposing zymolyte Water soluble fertilizer
Amount.Comparison rice growing way learns that the processing group for imposing zymolyte Water soluble fertilizer can effectively improve Rice Growing character, improving quality,
It is mainly shown as that the effective fringe of rice and mass of 1000 kernel increased.Compared with imposing common water-soluble fertilizer, water provided by the invention
Molten fertilizer can increase production 6.5~13.3%, illustrate that the present invention has good production-increasing function.
Influence of the 3 zymolyte fertilizer of table to rice yield
Embodiment 13
Zymolyte provided by the invention and the auxiliary agent containing the zymolyte are added in 1% ratio in Water soluble fertilizer and obtained
Zymolyte Water soluble fertilizer.The Water soluble fertilizer of the present invention is also achieved into good application effect on the industrial crops such as celery.Same
In the case that nutrient equivalent routine composite fertilizer base is applied, respectively use blank clear water control, etc. nutrients routine Water soluble fertilizer, etc. nutrients
Zymolyte Water soluble fertilizer carries out different groups of other top dressing processing.As shown in Table 4, the celery plant plant height of zymolyte Water soluble fertilizer processing
Highest, stem number is most, and yield is maximum.Compared with common Water soluble fertilizer, zymolyte Water soluble fertilizer can increase production 6.8~17.3%, illustrate this
Invention can more promote the overall growth of celery, have good production-increasing function.
Influence of the 4 zymolyte Water soluble fertilizer of table to celery yield
Embodiment 14
Cell experiment is carried out by experimental subjects of wheat, by product made from 1~embodiment of embodiment 10) by 10% ratio
Example, which is added in Water soluble fertilizer, obtains Water soluble fertilizer provided by the invention.The Water soluble fertilizer of the present invention is tested for cell wheat, experiment
12 processing are devised, it is conventional composite fertilizer (N-P-K that processing base, which is applied,:15-9-6) 40kg/ mus, difference lies in wheat bootings
Phase, pustulation period processing are different, and respectively (1) spray clear water, (2) spray conventional Water soluble fertilizer (N-P-K:28-12-12), (3~
12) the corresponding fertilizer (N-P-K of product made from 1~embodiment of the equal nutrients embodiment of the present invention 10 is sprayed:28-12-12+ is digested
Object fertilizer synergistic auxiliary agent).10 meters of zone leader, 4 meters wide, area 40m2, surrounding, which is set, protects row.Comparison different disposal is learnt same
In the case of nutrient fertilising, the wheat growing way of zymolyte Water soluble fertilizer processing is best, as shown in Table 5, Water soluble fertilizer provided by the invention
Plant height, grain number per spike, mass of 1000 kernel and the yield of wheat can be improved.Compared with common Water soluble fertilizer, Water soluble fertilizer provided by the invention can
Volume increase 1.6~4.3% illustrates that the present invention has certain production-increasing function in wheat using upper.
Table 5 imposes influence of the zymolyte Water soluble fertilizer to wheat yield
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of zymolyte, which is characterized in that be 4~7 in pH value, temperature is 30~60 using plant as raw material
It is digested under conditions of DEG C.
2. preparation method according to claim 1, which is characterized in that the enzyme is cellulase, zytase, sweet dew are poly-
Mixture more than one or both of carbohydrase, galactomannans enzyme or pectase.
3. preparation method according to claim 1 or 2, which is characterized in that the plant is konjaku, agricultural crop straw or tree
Mixture more than one or both of wood.
4. preparation method according to any one of claims 1 to 3, which is characterized in that the time of the enzymolysis be 10~
20h。
5. zymolyte made from preparation method according to any one of claims 1 to 4.
6. zymolyte according to claim 5 plant supply nutrition, it is disease-resistant or degeneration-resistant in application.
7. zymolyte according to claim 5 prepare fertilizer synergist, soil conditioner, microbial growth promoters,
Application in seed soaking agent, biological pesticide, Special Fertilizers for Various Crops.
8. a kind of auxiliary agent, which is characterized in that including zymolyte as claimed in claim 5.
9. a kind of fertilizer, which is characterized in that including acceptable auxiliary material in zymolyte as claimed in claim 5 and fertilizer.
10. a kind of pesticide, which is characterized in that including acceptable auxiliary material in zymolyte as claimed in claim 5 and pesticide.
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