CN113519556A - Method for preparing plant growth promoter by oxidizing lignin persulfate - Google Patents
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Abstract
The invention discloses a method for preparing a plant growth promoter by oxidizing lignin persulfate, and belongs to the field of plant growth promoters. According to the method, enzymolysis lignin generated in a biological refining process of a lignocellulose raw material is used as a raw material, zero-valent iron is used for activating ammonium persulfate to generate free radicals with oxidizability, the mass ratio of the enzymolysis lignin, the ammonium persulfate and the zero-valent iron is 1.00-1.50: 0.5: 0.15, the enzymolysis lignin is degraded for 90-120 min at 25 ℃ and 150r/min, and the obtained degraded supernatant can be used for promoting plant growth. According to the invention, the enzymatic hydrolysis lignin is subjected to oxidative degradation by a green and mild method, and when the total phenol content in the obtained supernatant is 20-40 mg/L, the promotion effect on the germination of rice seeds and the growth of overground parts is more obvious than that of commercial humic acid, so that a new way is provided for the utilization of lignin and the preparation of a plant growth promoter.
Description
Technical Field
The invention belongs to the field of plant growth promoters, relates to the production of biomass liquid fuel, bio-based chemicals and biomass nano-material monomers by biodegradation or biotransformation of lignocellulose raw materials, and particularly relates to a method for preparing a plant growth promoter by oxidation of lignin persulfate.
Background
The wood fiber biomass resource is the most abundant renewable resource in the world and mainly comprises three parts of cellulose, hemicellulose and lignin, wherein the lignin is the most abundant organic high molecular compound of secondary cellulose in the plant world and is often used as waste due to the complex structure and heterogeneity of the lignin, so that the realization of the efficient utilization of the lignin is of great significance. Humins (HS) are generally considered to be multiphase organic mixtures containing various aromatic and aliphatic groups. The unique effect of HS on crop productivity is their direct stimulation of plant growth. The structural similarity and difference of the lignin and HS show that the artificial transformation process can be used for enabling the lignin structure to simulate the coal-based commodity HS, and the lignin structure can be used as a plant growth promoter to be applied to the fields of agriculture, forestry and the like after being changed, so that a new way is provided for the utilization of the lignin.
The lignin artificial humification process may be based on microbial or non-biological reactions of lignin-related materials. The advanced oxidation for generating active oxygen can be combined with lignin humification, and the advanced oxidation technology mainly comprises a Fenton oxidation method, a photocatalytic oxidation method, an electrocatalytic oxidation method, an ozone oxidation method, an ultrasonic oxidation method, a wet air oxidation method, a supercritical water oxidation method and the like, but all the methods have the advantages and the disadvantages, so that a method for preparing the plant growth promoter by degrading lignin is found, which has good oxidation effect, is green and environment-friendly and has simple operation conditions, and is necessary for realizing the efficient utilization of the lignin.
Disclosure of Invention
Aiming at the defects in the prior art, the technical problem to be solved by the invention is to provide a method for preparing a plant growth promoter by oxidizing lignin persulfate.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method for preparing a plant growth promoter by oxidizing lignin persulfate comprises the following steps: enzymolysis lignin generated in the process of biologically refining the lignocellulose raw material is used as a raw material, zero-valent iron is used for activating ammonium persulfate to generate oxidizing free radicals to degrade the enzymolysis lignin, and the obtained degradation supernatant can be used for promoting plant growth.
Based on sulfate radicals (SO)4 ·-) The advanced oxidation technology is a novel advanced oxidation technology which is formed in recent years and has the advantages of high efficiency, short period, easy control of operation conditions and the like. The sulfate radical generation mode mainly comprises thermal activation, transition metal activation, microwave activation, illumination, ultrasonic activation and the like. Among them, the transition metal activation method can be classified into a homogeneous activation method (i.e., transition metal ions) and a heterogeneous activation method (i.e., solid metals and metal oxides), in which metal ions generate active radicals by breaking O — O bonds in persulfate to oxidatively degrade organic substances, and the metal ions that can be used to activate persulfate include Fe2+、Cu2+、Co2+And the like.
The persulfate oxidation method is a zero-valent iron activated ammonium persulfate method based on sulfate radicals, and adopts zero-valent iron to mainly enable liquid after reaction to contain more ferrous ions, so that the ferrous ions are utilized to promote plant growth.
No growth promoting effect on plant growth was reported for ferric iron because divalent iron activation would produce more ferric iron.
Further, the method specifically comprises the following steps: mixing enzymatic hydrolysis lignin and an ammonium persulfate solution, adding zero-valent iron powder to form an oxidative degradation reaction system, controlling reaction conditions until the reaction is finished, adding methanol to terminate the reaction, sucking out the residual zero-valent iron powder, centrifuging to perform solid-liquid separation, and respectively collecting supernatant and solid residues, wherein the obtained degradation supernatant can be used for promoting plant growth; the mass ratio of the enzymatic hydrolysis lignin, the ammonium persulfate and the zero-valent iron is 0.25-2.00: 0-1.25: 0-0.15; the reaction conditions are 25 ℃ and 150r/min for 15-120 min.
The method adopts a 50mL reaction system, mixes enzymolysis lignin (0.25-2.00 g) with ammonium persulfate solutions with different concentrations (0-25 g/L), adds zero-valent iron powder (0-0.15 g), immediately starts the reaction, and places the reaction in a constant-temperature oscillating water bath kettle to react for 15-120 min at the temperature of 25 ℃ and under the condition of 150 r/min. After the reaction is finished, adding 50 mu L of methanol to terminate the reaction, sucking out the residual zero-valent iron powder, centrifuging at 8000r/min for 5min to perform solid-liquid separation, washing the solid twice, and respectively collecting the supernatant and the solid residues. In actual operation, the modification can be carried out according to specific requirements.
Further, the mass ratio of the enzymatic hydrolysis lignin, the ammonium persulfate and the zero-valent iron is 1.00-1.50: 0.5: 0.15.
Further, the concentration of ammonium persulfate in the oxidative degradation reaction system is 10 g/L. When the addition amount of ammonium persulfate is 10g/L, the total phenol content is highest, and the pH of the supernatant liquid is gradually reduced along with the increase of the addition amount of ammonium persulfate.
Further, the reaction time is 90-120 min. When the reaction time reaches 90min, the measured total phenol content of the supernatant is the highest, and when the reaction is continued for 120min, the total phenol content in the supernatant is slightly reduced.
Further, the solid-liquid separation is carried out by centrifugation at 8000r/min for 5 min.
After the reaction is finished, adding methanol to reduce the residual oxidant in the reaction system, wherein the volume ratio of the methanol oxidation degradation reaction system is 1: 1000.
After the total phenol content of the degraded supernatant is measured by a Folin-Ciocalteu method, the degraded supernatant is diluted to make the total phenol content be 20 or 40mg/L for a germination experiment of rice seeds, commercial Humic Acid (HA) and distilled water are used for comparison, and the degraded supernatant obtained after lignin oxidation can promote plant growth.
Has the advantages that: compared with the prior art, the invention has the advantages that:
1) the invention provides a method for preparing a plant growth promoter by oxidizing lignin persulfate, aiming at the problem of utilizing residue-enzymatic hydrolysis lignin generated in the process of biorefinery of lignocellulose raw materials, which can effectively degrade lignin and obtain a product with a promoting effect on plant growth, and simultaneously, monophenolic substances for inhibiting plant growth are not generated or are generated as little as possible in the process of oxidative degradation.
2) The method adopts a green and mild method to carry out oxidative degradation on the enzymatic hydrolysis lignin, on one hand, the oxidation reaction is finished at the room temperature of 25 ℃, on the other hand, the zero-valent iron powder is sucked out after the reaction, and the reaction condition is mild and safe.
3) The effect of the supernatant obtained after lignin oxidation on promoting plant growth is very obvious, when the total phenol content in the supernatant is 20-40 mg/L, the effect of the supernatant on rice seed germination and overground part growth promotion is more obvious than that of commercial HA, and a new way is provided for lignin utilization and plant growth promoter preparation.
Drawings
FIG. 1 is a standard curve diagram established for determining the total phenol content in a liquid by Folin-Ciocalteu colorimetry;
FIG. 2 is a diagram showing the daily germination of rice seeds;
FIG. 3 is a graph showing the growth of rice on days 1, 3, 5 and 7;
FIG. 4 is a phenotype map of rice after 7 days of culture.
Detailed Description
The invention is further described with reference to specific examples. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention. Modifications or substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and scope of the invention. In the following examples, unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Example 1 enzymatic Lignin harvesting and processing
The enzymatic hydrolysis lignin is obtained by carrying out dilute acid steam explosion pretreatment (0.50-1.00% of sulfuric acid is soaked and dehydrated to reach the solid content of 30-40%, and then the solid content is treated for 5-10 min under the condition of 1.6-2.0 MPa), carrying out solid-liquid separation, washing solid residues, and further dehydrating the residues obtained by hydrolysis by adopting cellulase, thus obtaining the enzymatic hydrolysis lignin.
After the enzymolysis lignin is ground to 40-80 meshes by a grinder, the water content of the enzymolysis lignin is 7.64%, and the mass fractions of cellulose, hemicellulose and lignin are 15.93%, 3.20% and 58.63% respectively.
Example 2 persulfate Oxidation treatment of enzymatically hydrolyzed Lignin
(1) Determination of ammonium persulfate concentration
Weighing 1.00g of enzymatic hydrolysis lignin in 6 triangular flasks with the volume of 100mL, adding distilled water and ammonium persulfate mother liquor (the concentration of 25g/L) with the total volume of 50mL to enable the concentration of ammonium persulfate to be 0, 5, 10, 15, 20 and 25g/L respectively, uniformly mixing, adding 0.15g of zero-valent iron powder, sealing by using a preservative film, immediately starting reaction, placing in a constant-temperature oscillation water bath, and reacting for 120min at the temperature of 25 ℃ and at the speed of 150 r/min. After the reaction is finished, adding 50 mu L of methanol to terminate the reaction, sucking out the residual zero-valent iron powder, centrifuging at 8000r/min for 5min, carrying out solid-liquid separation, washing the solid twice, and respectively collecting the supernatant and the solid residues.
Evaluation of the Oxidation Effect: performing component analysis on the supernatant collected after lignin oxidation reaction, adopting High Performance Liquid Chromatography (HPLC) analysis, measuring the pH of the supernatant by using a pH meter and measuring the total phenol content in the supernatant by using a Folin-Ciocalteu colorimetric method, and referring to a diagram 1, a standard curve chart established for measuring the total phenol content in liquid by using the Folin-Ciocalteu colorimetric method is shown, wherein the experimental conditions are that 1mL of gallic acid standard liquid with different concentrations is added with 1mL of 0.6mol/L forrin phenol reagent, 5mL of distilled water and 3mL of 6% Na in sequence2CO3Mixing the solutions, reacting in 30 deg.C water bath for 120min, measuring absorbance at 745nm wavelength, andand drawing a standard curve of the total phenol content by taking a light absorption value (x) at 745nm as a horizontal coordinate and the concentration (y) of the gallic acid standard solution as a vertical coordinate, and obtaining a linear regression equation: 92.141x-0.6849, R2＝0.9979。
TABLE 1 Effect of ammonium persulfate concentration on Lignin Oxidation Effect
| Ammonium persulfate addition amount/(g/L) | Total phenol content/(mg/L) | |
| 0 | 129.95±10.10 | 5.83 |
| 5 | 696.19±29.97 | 3.62 |
| 10 | 1179.47±31.28 | 2.45 |
| 15 | 737.19±7.17 | 1.87 |
| 20 | 501.77±0.00 | 1.82 |
| 25 | 110.39±0.85 | 1.74 |
As can be seen from Table 1, the total phenol content is highest at 10g/L ammonium persulfate, which can reach 1179.47 + -31.28 mg/L. The pH of the supernatant gradually decreased as the amount of ammonium persulfate added increased.
When the concentration of ammonium persulfate is 10g/L, the supernatant is analyzed by HPLC to obtain: 1.3mg/L of xylose, 58mg/L of formic acid and 26mg/L of acetic acid; content of lignin degradation products: syringic acid 0.34mg/L, vanillin 2.14 mg/L. It can be seen that the contents of sugar and acid are both very low, and are probably degradation products of hemicellulose remained in the enzymatic hydrolysis lignin material, the concentration of monophenol of the lignin degradation products is very low, and the total phenol content is high, so that the condition that the phenol oligomer exists in the supernatant can be obtained, and therefore 10g/L is selected as the adding amount of ammonium persulfate.
(2) Determination of the Oxidation reaction time
Weighing 1.00g of enzymatic hydrolysis lignin in 6 triangular flasks of 100mL respectively, adding 10g/L of ammonium persulfate, the total volume of 50mL, adding 0.15g of zero-valent iron powder, immediately starting the reaction after sealing the preservative film, placing the mixture in a constant-temperature oscillating water bath, and finishing the reaction after 15 min, 30 min, 45 min, 60 min, 90min and 120min respectively at 25 ℃ and 150 r/min. After the reaction is finished, adding 50 mu L of methanol to terminate the reaction, sucking out the residual zero-valent iron powder, centrifuging at 8000r/min for 5min, carrying out solid-liquid separation, washing the solid twice, and respectively collecting the supernatant and the solid residues.
Evaluation of the Oxidation Effect: the supernatant collected after lignin oxidation was subjected to compositional analysis, the total phenol content in the supernatant was determined by High Performance Liquid Chromatography (HPLC) analysis, Folin-Ciocalteu colorimetry, and the pH of the supernatant was determined by a pH meter. The results are shown in Table 2.
TABLE 2 Effect of reaction time on lignin oxidation
| Reaction time/min | Total phenol content/(mg/L) | pH |
| 15 | 504.99±3.26 | 2.26 |
| 30 | 643.21±0.65 | 2.31 |
| 45 | 528.03±11.08 | 2.28 |
| 60 | 817.35±22.80 | 2.42 |
| 90 | 1186.38±23.46 | 2.55 |
| 120 | 1179.47±31.28 | 2.45 |
As can be seen from Table 2, the highest total phenol content of the supernatant was found to be 1186.38. + -. 23.46mg/L when the reaction time reached 90 min. Within 90min of reaction, the pH of the supernatant increases along with the increase of the reaction time, when the reaction is continued for 120min, the total phenol content in the supernatant is slightly reduced (1179.47 +/-31.28 mg/L), the pH is also slightly reduced, and the generated phenols are further degraded into acids probably due to excessive reaction, so that the pH of the supernatant is reduced.
When the reaction time was 90min, the supernatant was analyzed by HPLC to give: 1.1mg/L of xylose, 50mg/L of formic acid, 44mg/L of acetic acid, and the content of lignin degradation products: syringic acid 0.81mg/L, vanillin 1.59 mg/L. The obtained sugar and acid contents are very low, and are probably degradation products of residual hemicellulose in the enzymatic hydrolysis lignin material, the concentration of monophenols of the lignin degradation products is very low, the total phenol content is high, so that the main phenolic oligomers in the supernatant can be obtained, and 90-120 min is selected as the oxidation reaction time.
(3) Determination of addition amount of zero-valent iron powder
Respectively weighing 1.00g of enzymatic hydrolysis lignin in 6 triangular flasks with the volume of 50mL and the addition of 10g/L ammonium persulfate, respectively adding 0, 0.03, 0.06, 0.09, 0.12 and 0.15g of zero-valent iron powder, respectively, sealing the preservative film, immediately starting the reaction, placing the mixture in a constant-temperature oscillating water bath, and finishing the reaction at 25 ℃ and 150r/min after 90 min. After the reaction is finished, adding 50 mu L of methanol to terminate the reaction, sucking out the residual zero-valent iron powder, centrifuging at 8000r/min for 5min, carrying out solid-liquid separation, washing the solid twice, and respectively collecting the supernatant and the solid residues.
Evaluation of the Oxidation Effect: the supernatant collected after lignin oxidation was subjected to compositional analysis, the total phenol content in the supernatant was determined by High Performance Liquid Chromatography (HPLC) analysis, Folin-Ciocalteu colorimetry, and the pH of the supernatant was determined by a pH meter. The results are shown in Table 3.
TABLE 3 influence of the addition of zero-valent iron powder on the Oxidation Effect of Lignin
| Addition amount of zero-valent iron powder/g | Total phenol content/(mg)/L) | |
| 0 | 83.85±0.33 | 3.85 |
| 0.03 | 46.68±2.61 | 1.77 |
| 0.06 | 51.56±0.78 | 1.77 |
| 0.09 | 522.50±7.17 | 2.01 |
| 0.12 | 730.28±23.46 | 2.10 |
| 0.15 | 1179.47±31.28 | 2.45 |
As can be seen from Table 3, the total phenol content of the supernatant liquid gradually increased as the amount of the zero-valent iron powder added increased. When the addition amount of the zero-valent iron powder is 0.15g, the pH value of the supernatant is 2.45, and the total phenol content is the highest and can reach 1179.47 +/-31.28 mg/L, so 0.15g is selected as the addition amount of the zero-valent iron powder.
(4) Determination of addition amount of enzymatic hydrolysis lignin
Respectively weighing 0.25 g, 0.50 g, 1.00g, 1.50g and 2.00g of enzymatic hydrolysis lignin in 5 triangular bottles with the volume of 50mL and the addition of 10g/L of ammonium persulfate, adding 0.15g of zero-valent iron powder, immediately starting the reaction after the preservative film is sealed, placing the mixture in a constant-temperature oscillation water bath, keeping the temperature at 25 ℃ for 150r/min, and finishing the reaction after 90min or 120 min. After the reaction is finished, adding 50 mu L of methanol to terminate the reaction, sucking out the residual zero-valent iron powder, centrifuging at 8000r/min for 5min, carrying out solid-liquid separation, washing the solid twice, and respectively collecting the supernatant and the solid residues.
Evaluation of the Oxidation Effect: the supernatant collected after lignin oxidation was subjected to compositional analysis, the total phenol content in the supernatant was determined by High Performance Liquid Chromatography (HPLC) analysis, Folin-Ciocalteu colorimetry, and the pH of the supernatant was determined by a pH meter. The results are shown in Table 4.
TABLE 4 influence of lignin addition on lignin oxidation effect
| Addition amount/g of enzymolysis lignin | Total phenol content/(mg/L) | pH |
| 0.25 | 21.89±4.30 | 2.03 |
| 0.50 | 927.46±7.82 | 2.33 |
| 1.00 | 1179.47±31.28 | 2.45 |
| 1.50 | 1247.65±18.89 | 2.47 |
| 2.00 | 1185.92±44.96 | 2.32 |
As can be seen from Table 4, the total phenol content in the supernatant after the reaction tended to increase and then decrease as the amount of the enzymatic lignin added increased. When the addition amount of the enzymatic hydrolysis lignin is 1.50g, the total phenol content reaches the highest value, namely 1247.65 +/-18.89 mg/L, at the moment, the pH value of the supernatant is 2.47 at the maximum, and when the addition amount of the enzymatic hydrolysis lignin reaches 2.00g, the total phenol content in the supernatant is reduced on the contrary. When the addition amount of the enzymatic hydrolysis lignin is more than 1.00g, the increase range of the total phenol content in the supernatant is not large, so 1.00-1.50 g is selected as the addition amount of the enzymatic hydrolysis lignin.
Example 3 supernatant collected after oxidation of Lignin persulfate for germination and early culture of Rice seeds
1) Preparation of treatment liquid: and diluting the supernatant obtained under the optimal oxidation conditions (1.00-1.50 g of enzymatic hydrolysis lignin, 10g/L of ammonium persulfate, 0.15g of zero-valent iron powder, 50mL of reaction system, reaction time of 90-120 min, water bath temperature of 25 ℃, and 150r/min) until the total phenol content is 20mg/L and 40mg/L respectively, and preparing two commercial HA solutions with the same total phenol content for later use.
2) Germinating rice seeds: the rice seeds were soaked in 70% ethanol for 8min, 2.5% (v/v) sodium hypochlorite for 5min, washed with distilled water for 5 times, sterilized, transferred to 9cm petri dishes with tweezers, 15 rice seeds were placed in each petri dish, 5mL of the above four liquids were added, and distilled water was used as a blank Control (CK) and the measurement was repeated two times each time. Accelerating germination in the dark at 28 ℃ in an incubator, and finishing the culture for 7 days.
3) Determination of rice growth indexes: the germination vigor is calculated in 3 days, the germination rate and the germination index are calculated in 7 days, and the plant height, the main root length, the fresh weight of the overground part and the underground part are measured. Wherein, the germination potential is the number of the germinated seeds when the number of the germinated seeds is the highest in a day/the total number of the tested seeds multiplied by 100 percent, the germination rate is the number of the seeds at the end of germination/the total number of the tested seeds multiplied by 100 percent, and the germination index is the sum of the ratio of the number of the germinated seeds per day to the number of the germinated days.
The germination number of the rice seeds is recorded every day, the daily germination rate of the rice seeds is calculated, and is shown in figure 2, and the germination process of the rice seeds and the phenotype of the rice seeds at the 7 th day are respectively shown in figure 3 and figure 4. The germination results of rice seeds are shown in table 5, and the growth indexes of rice seedlings are shown in table 6.
TABLE 5 Rice seed Germination results
| Sample (I) | Germination potential/%) | Germination rate/% | Index of germination |
| CK | 71.11±7.70 | 77.78±3.85 | 16.67±0.46 |
| Supernatant-20 | 90.00±4.71 | 93.33±9.43 | 21.26±1.49 |
| Supernatant-40 | 70.00±14.14 | 80.00±9.43 | 17.11±3.55 |
| HA-20 | 68.48±9.43 | 80.36±4.71 | 14.55±2.07 |
| HA-40 | 72.52±14.14 | 77.38±9.43 | 13.77±2.92 |
TABLE 6 growth indices of rice seedlings
| Sample (I) | Plant height/cm | Length of main root/cm | Fresh weight of aerial parts/g | Fresh weight of underground part/g |
| CK | 2.50±0.24 | 4.31±0.56 | 0.0124±0.0027 | 0.0034±0.0012 |
| Supernatant-20 | 2.83±0.16 | 3.47±0.69 | 0.0135±0.0037 | 0.0021±0.0018 |
| Supernatant-40 | 3.14±0.14 | 2.33±0.36 | 0.0162±0.0041 | 0.0029±0.0027 |
| HA-20 | 2.56±0.20 | 5.10±1.07 | 0.0100±0.0014 | 0.0026±0.0004 |
| HA-40 | 2.55±0.22 | 3.78±1.17 | 0.0108±0.0011 | 0.0026±0.0004 |
The results show that: after the supernatant is used for germinating the rice seeds, when the total phenol content is 20mg/L, the germination potential, the germination rate and the germination index of the rice seeds are respectively increased by 26.56%, 19.99% and 27.53% compared with the blank, and the plant height of seedlings and the overground fresh weight of the seedlings are respectively increased by 13.20% and 8.87%; when the total phenol content is 40mg/L, the germination potential of the rice seeds is similar to that of the blank, the germination rate and the germination index are respectively increased by 2.85 percent and 2.64 percent compared with the blank, and the plant height of the bud seedlings and the ground fresh weight are respectively increased by 25.60 percent and 30.65 percent compared with the blank. The supernatant does not show obvious promotion effect on the roots of the rice, the result is similar to the effect result of the commodity HA, but the promotion effect of the supernatant on the germination of the rice seeds and the overground part of the rice seeds is more obvious than that of the commodity HA, the commodity HA slightly promotes the root length of the rice, and other indexes do not show obvious promotion effect. Therefore, the supernatant showed a growth promoting effect on rice, and the effect was more remarkable than that of commercial HA.
Claims (8)
1. A method for preparing a plant growth promoter by oxidizing lignin persulfate is characterized by comprising the following steps: enzymolysis lignin generated in the process of biologically refining the lignocellulose raw material is used as a raw material, zero-valent iron is used for activating ammonium persulfate to generate oxidizing free radicals to degrade the enzymolysis lignin, and the obtained degradation supernatant can be used for promoting plant growth.
2. The method for preparing the plant growth promoter by oxidizing the lignin persulfate according to claim 1, which comprises the following steps: mixing enzymatic hydrolysis lignin and an ammonium persulfate solution, adding zero-valent iron powder to form an oxidative degradation reaction system, controlling reaction conditions until the reaction is finished, adding methanol to terminate the reaction, sucking out the residual zero-valent iron powder, centrifuging to perform solid-liquid separation, and respectively collecting supernatant and solid residues, wherein the obtained degradation supernatant can be used for promoting plant growth; the mass ratio of the enzymatic hydrolysis lignin, the ammonium persulfate and the zero-valent iron is 0.25-2.00: 0-1.25: 0-0.15; the reaction conditions are 25 ℃ and 150r/min for 15-120 min.
3. The method for preparing the plant growth promoter by oxidizing the lignin persulfate according to claim 2, wherein the mass ratio of the enzymatic hydrolysis lignin, the ammonium persulfate and the zero-valent iron is 1.00-1.50: 0.5: 0.15.
4. The method for preparing the plant growth promoter by oxidizing the lignin persulfate according to claim 2, wherein the concentration of the ammonium persulfate in the oxidative degradation reaction system is 10 g/L.
5. The method for preparing the plant growth promoter by oxidizing the lignin persulfate according to claim 2, wherein the reaction time is 90-120 min.
6. The method for preparing the plant growth promoter by oxidizing the lignin persulfate according to claim 2, wherein the solid-liquid separation is performed by centrifugation at 8000r/min for 5 min.
7. The method for preparing a plant growth promoter by oxidizing lignin persulfate as claimed in claim 2, wherein the volume ratio of methanol to the oxidative degradation reaction system is 1: 1000.
8. The method for preparing the plant growth accelerant by oxidizing the lignin persulfate according to the claim 2, wherein the total phenol content in the degradation supernatant for promoting the plant growth is 20-40 mg/L respectively.
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