CN108330111A - 细胞色素p450突变蛋白及其应用 - Google Patents
细胞色素p450突变蛋白及其应用 Download PDFInfo
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Abstract
Description
最初的残基 | 代表性的取代 | 优选的取代 |
Ala(A) | Val;Leu;Ile | Val |
Arg(R) | Lys;Gln;Asn | Lys |
Asn(N) | Gln;His;Lys;Arg | Gln |
Asp(D) | Glu | Glu |
Cys(C) | Ser | Ser |
Gln(Q) | Asn | Asn |
Glu(E) | Asp | Asp |
Gly(G) | Pro;Ala | Ala |
His(H) | Asn;Gln;Lys;Arg | Arg |
Ile(I) | Leu;Val;Met;Ala;Phe | Leu |
Leu(L) | Ile;Val;Met;Ala;Phe | Ile |
Lys(K) | Arg;Gln;Asn | Arg |
Met(M) | Leu;Phe;Ile | Leu |
Phe(F) | Leu;Val;Ile;Ala;Tyr | Leu |
Pro(P) | Ala | Ala |
Ser(S) | Thr | Thr |
Thr(T) | Ser | Ser |
Trp(W) | Tyr;Phe | Tyr |
Tyr(Y) | Trp;Phe;Thr;Ser | Phe |
Val(V) | Ile;Leu;Met;Phe;Ala | Leu |
SEQ ID NO. | 备注 | PPD/DM提高 | |
optPPDS | 1 | 野生型 | 0 |
1G4 | 2 | L87I | 53.2% |
2D8 | 3 | K235R | 57.7% |
3B9 | 4 | K349R,V366I | 80.2% |
8G7 | 5 | N231Y,S285C | 26.1% |
9C1 | 6 | P91H | 84.20% |
16F8 | 7 | Q113R | 30.6% |
24A10 | 8 | 第1-4位氨基酸缺失,L18I | 125.6% |
SEQ ID NO. | 备注 | PPD/DM提高 | |
GAL1 | 9 | 野生型启动子 | 0 |
1D1 | 10 | SEQ ID NO.:9中发生突变:C417G,A445G | 44.1% |
9C1-2 | 11 | SEQ ID NO.:9中发生突变:第28位T缺失 | 80.1% |
11B5 | 12 | SEQ ID NO.:9中发生突变:G654A | 62.2% |
15F1 | 13 | SEQ ID NO.:9中发生突变:G655A | 44.1% |
Claims (10)
Priority Applications (4)
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CN109295027A (zh) * | 2018-11-06 | 2019-02-01 | 浙江华睿生物技术有限公司 | 一种糖基转移酶突变体 |
CN109468287A (zh) * | 2018-11-22 | 2019-03-15 | 浙江华睿生物技术有限公司 | 一种羟化酶突变体 |
CN113388590A (zh) * | 2021-06-07 | 2021-09-14 | 山西农业大学 | 一种细胞色素P450s的突变体 |
CN114507646A (zh) * | 2020-11-17 | 2022-05-17 | 中国科学院分子植物科学卓越创新中心 | 细胞色素p450突变体蛋白及其应用 |
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CN109295027A (zh) * | 2018-11-06 | 2019-02-01 | 浙江华睿生物技术有限公司 | 一种糖基转移酶突变体 |
CN109468287A (zh) * | 2018-11-22 | 2019-03-15 | 浙江华睿生物技术有限公司 | 一种羟化酶突变体 |
CN114507646A (zh) * | 2020-11-17 | 2022-05-17 | 中国科学院分子植物科学卓越创新中心 | 细胞色素p450突变体蛋白及其应用 |
WO2022105729A1 (zh) * | 2020-11-17 | 2022-05-27 | 中国科学院分子植物科学卓越创新中心 | 细胞色素p450突变体蛋白及其应用 |
CN114507646B (zh) * | 2020-11-17 | 2023-11-10 | 生合万物(上海)生物科技有限公司 | 细胞色素p450突变体蛋白及其应用 |
CN113388590A (zh) * | 2021-06-07 | 2021-09-14 | 山西农业大学 | 一种细胞色素P450s的突变体 |
CN113388590B (zh) * | 2021-06-07 | 2022-02-25 | 山西农业大学 | 一种细胞色素P450s的突变体 |
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KR102310518B1 (ko) | 2021-10-08 |
CN108330111B (zh) | 2023-01-31 |
JP7090266B2 (ja) | 2022-06-24 |
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