CN108324933A - Lysozyme disinfecting liquid and its preparation method and application - Google Patents
Lysozyme disinfecting liquid and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of lysozyme disinfecting liquid and its preparation method and application, lysozyme disinfecting liquid includes the raw material of following parts by weight：5 10 parts of 45 parts of lysozyme, 14 parts of polyhexamethylene arc, 34 parts of egg-shell meal, 6 10 parts of honeysuckle and onion, further include triethanolamine, and the pH that triethanolamine adjusts lysozyme disinfecting liquid is 5.0 6.0.The present invention have wide sterilization spectrum, skin irritation it is small, without pigmenting of skin problem, and persistence is strong, has the advantageous effect of certain inhibiting effect for gram-negative bacteria.
Description
Technical field
The present invention relates to thimerosal technical fields.It is more particularly related to a kind of lysozyme disinfecting liquid and its system
Preparation Method and application.
Background technology
Bacterium on skin can be divided into transient bacterium and resident bacteria, and resident bacteria usually lives in keratoderma epithelial cell
In the following, it is seldom related with infection, transient bacterium cortex on the skin, in clinical treatment nursery work, transient bacterium usually passes through
Medical staff and patient are in direct contact and obtain or contact the environmental surfaces near patient and pollute, so medical staff is carrying out
Sterile working must be strictly observed when operations, especially surgery contact patient relatively frequently, occur infection probability it is opposite
Big medical staff more needs to abide by sterile working.Therefore, clinical various treatments, diagnosis process are both needed to skin degerming, and disinfection is control
The important link of infection is made, existing skin antiseptic is mostly chemical reagent, has mainly had ethyl alcohol, iodine and Iodic agent, chlorine
Fixed, quaternary ammonium compound, triclosan etc., wherein the ethanol solution bactericidal effect of a concentration of 60%-80% is best, but its
Persistence is poor, and sterilization is general narrow, therefore ethanol solution, generally only as common sterilizing agent for external use, iodine is also clinical extensive with Iodic agent
The skin antiseptic used, but it is easy to colour on the skin with certain color, and the problem for causing elution difficult, chlorine is
Fixed it is weaker to the effect of gram-negative bacteria and fungi, and it is narrow that quaternary ammonium compound equally exists fungicidal spectrum, to gram-negative bacteria
Act on poor, although triclosan is a kind of high-efficiency broad spectrum antiseptic, but its bactericidal effect relative to ethyl alcohol, iodine and contains iodine system
Agent, chlorhexidine, quaternary ammonium compound are weaker；
Prepare it is a kind of have wide sterilization spectrum, skin irritation it is small, without pigmenting of skin problem, and persistence is strong, blue for leather
It is current urgent problem that negative bacterium, which has the disinfectant liquid for skin of certain inhibiting effect,.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of lysozyme disinfecting liquid, wide sterilization spectrum, skin irritation be small, without skin
Skin coloring problem, and persistence is strong, has certain inhibiting effect for gram-negative bacteria.
It is a still further object of the present invention to provide a kind of preparation methods of lysozyme disinfecting liquid, use at gradient supercharging
Reason so that the Fungicidal substance in honeysuckle and onion obtains extraction as much as possible, and the repetitious freezing heating of mixing slag
Processing, the same interior Fungicidal substance of reservation mixing as much as possible, effectively improves the sterilizing ability of lysozyme disinfecting liquid.
It is a still further object of the present invention to provide a kind of application of lysozyme disinfecting liquid in skin degerming.
In order to realize these purposes and other advantages according to the present invention, a kind of lysozyme disinfecting liquid is provided, including with
The raw material of lower parts by weight：4-5 parts of lysozyme, 1-4 parts of polyhexamethylene arc, 3-4 parts of egg-shell meal, 6-10 parts of honeysuckle and onion
5-10 parts.
Preferably, lysozyme disinfecting liquid further includes：Triethanolamine, the pH that triethanolamine adjusts lysozyme disinfecting liquid are
5.0-6.0。
The present invention also provides a kind of preparation methods of lysozyme disinfecting liquid, include the following steps：
Step 1: component meter by weight, takes 5-10 parts of onion, the onion of half parts by weight to obtain ocean with steam distillation
Green onion essential oil and bottoms；
It takes eggshell to crushed 400 mesh sieve, removes sieving, 400 mesh on-the-sieve materials continued 200 mesh sieve, and obtained egg-shell meal, take
3-4 parts of egg-shell meal, the egg-shell meal of a quarter parts by weight is placed in onion essential oil, and stirring makes it be uniformly mixed, and is stood 3-4h, is obtained
Essential oil powder mixture；
Step 2: taking 4-5 parts of lysozyme, water is added, stirring makes lysozyme just be completely dissolved, obtains lysozyme water, take egg
2/4ths parts by weight of shell powder are placed in lysozyme water, and swaying makes it be uniformly mixed, and are stood 1-1.2h, are obtained bacteriolyze enzymatic mixture；
1-4 parts of polyhexamethylene arc is taken, water is added, stirring makes polyhexamethylene arc just be completely dissolved, obtains poly- six methylene
Remaining egg-shell meal is placed in polyhexamethylene arc water by base curve water, and swaying makes it be uniformly mixed, and stands 1-1.2h, and it is sub- to obtain poly- six
Bacteriolyze enzymatic mixture is uniformly mixed with polyhexamethylene arc mixture and stands 30-40min, obtains egg-shell meal by Methylguanidine mixture
Mixture；
Step 3: 6-10 parts of extracting honeysuckle, mashing, obtain honeysuckle slurry, honeysuckle slurry is placed in reaction kettle, gold and silver is added
The sieving that flower starches the lemon juice of 0.2 times of amount, 400 mesh of eggshell of 0.2 times of amount sieves after mixing, after carrying out pressurized treatments, drops
To normal pressure, crosses 500 mesh and sieve to obtain honeysuckle filtrate and honeysuckle filter residue；
The onion of the other half parts by weight is taken, is beaten, is obtained onion slurry, onion slurry is placed in reaction kettle, onion slurry 0.2 is added
The beer of amount after mixing, after carrying out pressurized treatments, is down to normal pressure, onion filtrate and onion filter residue is filtered to obtain, to onion again
The adsorbent of onion filtrate gross mass 10% is added in filtrate, stands 20-25min after stirring evenly, filters to obtain absorption filtrate；
Step 4: being mixed by bottoms, honeysuckle filter residue, with onion filter residue, mixing slag is obtained, mixing slag gross mass is added
The 2-3 times of water measured carries out 3 times repeatedly freezing heating treatments after being uniformly mixed, wherein freezing heating treatment is specially：
First be placed under -25 DEG C of environment and freeze 15-18min, take out postposition and thaw at room temperature, after heated up with the heating rate of 10 DEG C/min
To 50 DEG C and keep 6-8min, continue with the heating rate of 10 DEG C/min be warming up to 70 DEG C holding 6-8min, finally with 10 DEG C/
The heating rate of min is warming up to 100 DEG C of holding 6-8min, is down to room temperature, filters to obtain mixing filtrate；
Step 5: after mixing by honeysuckle filtrate, absorption filtrate and mixing filtrate, eggshell powder mixture being added, stirs
Mixing makes it be uniformly mixed, and stands 1-1.5h, and essential oil powder mixture is added, is uniformly mixed to get lysozyme disinfecting liquid.
Preferably, adsorbent is diatomite in step 3.
Preferably, essential oil powder mixture is added in step 5, triethanolamine is added after being uniformly mixed, adjusting pH is
5.0-6.0 obtains the lysozyme disinfecting liquid that pH is 5.0-6.0.
Preferably, in step 3, the specific method of pressurized treatments is to adjust reactor pressure to be 300kPa and keep 30-
40min, the pressure increased in reaction kettle keep 10-15min to 350kPa, and the pressure in increase reaction kettle to 380kPa keeps 6-
8min。
The present invention also provides a kind of purposes of above-mentioned lysozyme disinfecting liquid, purposes is, lysozyme disinfection
Application of the liquid in skin degerming.
The present invention includes at least following advantageous effect：
The first, eggshell itself belongs to the porous material with micropore, by egg-shell meal respectively with onion essential oil, lysozyme
Water and the mixing of polyhexamethylene arc water are stood, and can be partially absorbed and be stored the effective Fungicidal substance in part, slow down Fungicidal substance
Release dynamics, and then enhance the sterilization persistence of lysozyme disinfecting liquid, adsorption capacity and essential oil of the egg-shell meal for aqueous solution
Adsorption capacity is different, and then further be staggered extends the sterilization persistence ability of lysozyme disinfecting liquid, and the setting of egg-shell meal
The sterilization component of lysozyme anti-virus solution equally can effectively be improved in the attachment of egg-shell meal and cocoanut active charcoal circumferential direction, Jin Erjin
One step extends the action time of thimerosal on the skin；
The second, the sieving of lemon juice and 400 mesh of eggshell sieve is added in honeysuckle, and is mixed and used in last handling process
Gradient pressurized treatment so that honeysuckle cell damage is more effective, and Fungicidal substance obtains extraction as much as possible, is added in onion
Gradient pressurized treatment is used after beer, is increased onion cells breakage efficiency, can effectively be reduced onion penetrating odor, make sterilization
Substance extraction as much as possible, while the use of adsorbent further decrease the sense of discomfort that onion taste brings user, mixing
The repetitious freezing heating treatment of freezing of slag, same reservation bottoms, honeysuckle filter residue and onion as much as possible filter
Fungicidal substance in slag has apparent enhancing inhibiting effect for gram-negative bacteria, pathogen, shigella dysenteriae；
Third, honeysuckle and the addition of onion enhance tool facilitation for lysozyme disinfecting liquid sterilizing ability, while right
When ratio 2 initial 4min detections, sterilizing rate (4min clump counts after clump count/disinfection before disinfection) is more than 85%, wherein lysozyme
The alkaline enzyme that polysaccharide is sticked in pathogenic bacteria can be effectively hydrolyzed, by destroying -acetylmuramic acid and N- acetylaminos in cell wall
β-Isosorbide-5-Nitrae glycosidic bond between glucose makes the insoluble glutinous polysaccharide of cell wall resolve into soluble glycopeptide, leads to cell wall rupture content
Object escapes and bacterolysis is made to reach bactericidal effect, and polyhexamethylene arc can generate ionization, hydrophilic base portion in aqueous solution
Dividing has strong electropositive, and absorption is usually in that electronegative all kinds of bacteriums, virus inhibit film lactones plastid into cell membrane
Synthesis, causes thalline apoptosis, reaches effective sterilization, and the entirety that cooperation honeysuckle, onion further enhance lysozyme disinfecting liquid disappears
Toxic effect fruit.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Description of the drawings
Fig. 1 is the process flow chart of the preparation method of lysozyme disinfecting liquid described in the one of which embodiment of the present invention.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail, to enable those skilled in the art with reference to specification
Word can be implemented according to this.
<Embodiment 1>
Lysozyme disinfecting liquid includes the raw material of following parts by weight：4 parts of lysozyme, 1 part of polyhexamethylene arc, egg-shell meal 3
5 parts of part, 6 parts of honeysuckle and onion；
Further include：Triethanolamine, the pH that triethanolamine adjusts lysozyme disinfecting liquid are 5.5.
As shown in Figure 1, the preparation method of lysozyme disinfecting liquid, which is characterized in that include the following steps：
Step 1: component meter by weight, takes 5 parts of onion, the onion of half parts by weight to obtain onion with steam distillation
Essential oil and bottoms；
It takes eggshell to crushed 400 mesh sieve, removes sieving, the material on 400 mesh sieve continued 200 mesh sieve, and obtained egg-shell meal
(controlling the mesh number of egg-shell meal between 200-400), takes 3 parts of egg-shell meal, the egg-shell meal of a quarter parts by weight is placed in onion
In essential oil, stirring makes it be uniformly mixed, and stands 3h, obtains essential oil powder mixture；
Step 2: take 4 parts of lysozyme, add water, stirring make lysozyme be just completely dissolved (i.e. in operating process, room temperature
Water is slowly added into lysozyme down, and coordinates stirring, until when lysozyme is just completely dissolved, stops adding water), obtain lysozyme
Water takes 2/4ths parts by weight of egg-shell meal to be placed in lysozyme water, and swaying makes it be uniformly mixed, and stands 1h, obtains lysozyme mixing
Object；
Take 1 part of polyhexamethylene arc, add water, stirring make polyhexamethylene arc be just completely dissolved (i.e. in operating process,
Water is slowly added into polyhexamethylene arc at room temperature, and coordinates stirring, until when polyhexamethylene arc is just completely dissolved, is stopped
Add water), polyhexamethylene arc water is obtained, remaining egg-shell meal is placed in polyhexamethylene arc water, swaying makes it be uniformly mixed, and stands
1h obtains polyhexamethylene arc mixture, and bacteriolyze enzymatic mixture is uniformly mixed with polyhexamethylene arc mixture and stands 30min,
Obtain eggshell powder mixture；
Step 3: 6 parts of extracting honeysuckle, mashing, obtain honeysuckle slurry, honeysuckle slurry is placed in reaction kettle, honeysuckle is added
(eggshell smashes it through the eggshell that 400 mesh sieve to the sieving that the lemon juice of 0.2 times of amount of slurry, 0.2 times of 400 mesh of eggshell measured sieve
Crushed material) after mixing, after carrying out pressurized treatments, it is down to normal pressure, 500 mesh is crossed and sieves to obtain honeysuckle filtrate and honeysuckle filter residue；
The onion of the other half parts by weight is taken, is beaten, is obtained onion slurry, onion slurry is placed in reaction kettle, onion slurry 0.2 is added
The beer of amount after mixing, after carrying out pressurized treatments, is down to normal pressure, onion filtrate and onion filter residue is filtered to obtain, to onion again
The adsorbent of onion filtrate gross mass 10% is added in filtrate, stands 20min after stirring evenly, filters to obtain absorption filtrate, wherein inhales
Attached dose is diatomite, and the specific method of pressurized treatments is to adjust reactor pressure to be 300kPa and keep 30min, increases reaction kettle
Interior pressure keeps 10min to 350kPa, and the pressure in increase reaction kettle to 380kPa keeps 6min；
Step 4: being mixed by bottoms, honeysuckle filter residue, with onion filter residue, mixing slag is obtained, mixing slag gross mass is added
The water of 2 times of amounts, carries out 3 times repeatedly freezing heating treatments after being uniformly mixed, wherein freezing heating treatment is specially：First
Be placed under -25 DEG C of environment and freeze 15min, take out postposition and thaw at room temperature, after with the heating rate of 10 DEG C/min be warming up to 50
DEG C and keep 6min, continue to be warming up to 70 DEG C of holding 6min with the heating rate of 10 DEG C/min, finally with the heating of 10 DEG C/min
Rate is warming up to 100 DEG C of holding 6min, is down to room temperature, filters to obtain mixing filtrate；
Step 5: after mixing by honeysuckle filtrate, absorption filtrate and mixing filtrate, eggshell powder mixture being added, stirs
Mixing makes it be uniformly mixed, and stands 1h, and essential oil powder mixture is added, is uniformly mixed, and triethanolamine is added, and it is 5.5 to adjust pH,
The lysozyme disinfecting liquid for being 5.5 up to pH.
<Embodiment 2>
Lysozyme disinfecting liquid includes the raw material of following parts by weight：4.5 parts of lysozyme, 2.5 parts of polyhexamethylene arc, eggshell
7.5 parts of 3.5 parts of powder, 8 parts of honeysuckle and onion；
Further include：Triethanolamine, the pH that triethanolamine adjusts lysozyme disinfecting liquid are 5.6.
As shown in Figure 1, the preparation method of lysozyme disinfecting liquid, which is characterized in that include the following steps：
Step 1: component meter by weight, takes 7.5 parts of onion, the onion of half parts by weight to obtain ocean with steam distillation
Green onion essential oil and bottoms；
It takes eggshell to crushed 400 mesh sieve, removes sieving, 400 mesh on-the-sieve materials continued 200 mesh sieve, and obtained egg-shell meal, take
3.5 parts of egg-shell meal, the egg-shell meal of a quarter parts by weight is placed in onion essential oil, and stirring makes it be uniformly mixed, and is stood 3.5h, is obtained
Essential oil powder mixture；
Step 2: taking 4.5 parts of lysozyme, water is added, stirring makes lysozyme just be completely dissolved, obtains lysozyme water, take egg
2/4ths parts by weight of shell powder are placed in lysozyme water, and swaying makes it be uniformly mixed, and are stood 1.1h, are obtained bacteriolyze enzymatic mixture；
2.5 parts of polyhexamethylene arc is taken, water is added, stirring makes polyhexamethylene arc just be completely dissolved, obtains poly- six methylene
Remaining egg-shell meal is placed in polyhexamethylene arc water by base curve water, and swaying makes it be uniformly mixed, and is stood 1.5h, is obtained poly- six methylene
Bacteriolyze enzymatic mixture is uniformly mixed with polyhexamethylene arc mixture and stands 35min by base curve mixture, obtains egg-shell meal mixing
Object；
Step 3: 8 parts of extracting honeysuckle, mashing, obtain honeysuckle slurry, honeysuckle slurry is placed in reaction kettle, honeysuckle is added
The sieving that the lemon juice of 0.2 times of amount of slurry, 0.2 times of 400 mesh of eggshell measured sieve after mixing, after carrying out pressurized treatments, is down to
Normal pressure crosses 500 mesh and sieves to obtain honeysuckle filtrate and honeysuckle filter residue；
The onion of the other half parts by weight is taken, is beaten, is obtained onion slurry, onion slurry is placed in reaction kettle, onion slurry 0.2 is added
The beer of amount after mixing, after carrying out pressurized treatments, is down to normal pressure, onion filtrate and onion filter residue is filtered to obtain, to onion again
The adsorbent of onion filtrate gross mass 10% is added in filtrate, stands 22.5min after stirring evenly, filters to obtain absorption filtrate, wherein
Adsorbent is diatomite, and the specific method of pressurized treatments is to adjust reactor pressure to be 300kPa and keep 35min, increases reaction
Pressure in kettle keeps 12.5min to 350kPa, and the pressure in increase reaction kettle to 380kPa keeps 7min；
Step 4: being mixed by bottoms, honeysuckle filter residue, with onion filter residue, mixing slag is obtained, mixing slag gross mass is added
The water of 2.5 times of amounts, carries out 3 times repeatedly freezing heating treatments after being uniformly mixed, wherein freezing heating treatment is specially：
First be placed under -25 DEG C of environment and freeze 16.5min, take out postposition and thaw at room temperature, after heated up with the heating rate of 10 DEG C/min
To 50 DEG C and 7min is kept, continues to be warming up to 70 DEG C of holding 7min with the heating rate of 10 DEG C/min, finally with 10 DEG C/min's
Heating rate is warming up to 100 DEG C of holding 7min, is down to room temperature, filters to obtain mixing filtrate；
Step 5: after mixing by honeysuckle filtrate, absorption filtrate and mixing filtrate, eggshell powder mixture being added, stirs
Mixing makes it be uniformly mixed, and stands 1.25h, and essential oil powder mixture is added, is uniformly mixed, and triethanolamine is added, and adjusts pH and is
5.6 to get pH be 5.6 lysozyme disinfecting liquid.
<Embodiment 3>
Lysozyme disinfecting liquid includes the raw material of following parts by weight：5 parts of lysozyme, 4 parts of polyhexamethylene arc, egg-shell meal 4
10 parts of part, 10 parts of honeysuckle and onion；
Further include：Triethanolamine, the pH that triethanolamine adjusts lysozyme disinfecting liquid are 5.5.
As shown in Figure 1, the preparation method of lysozyme disinfecting liquid, which is characterized in that include the following steps：
Step 1: component meter by weight, takes 10 parts of onion, the onion of half parts by weight to obtain onion with steam distillation
Essential oil and bottoms；
It takes eggshell to crushed 400 mesh sieve, removes sieving, 400 mesh on-the-sieve materials continued 200 mesh sieve, and obtained egg-shell meal, take
4 parts of egg-shell meal, the egg-shell meal of a quarter parts by weight is placed in onion essential oil, and stirring makes it be uniformly mixed, and is stood 4h, is obtained essential oil
Powder mixture；
Step 2: taking 5 parts of lysozyme, water is added, stirring makes lysozyme just be completely dissolved, obtains lysozyme water, take eggshell
2/4ths parts by weight of powder are placed in lysozyme water, and swaying makes it be uniformly mixed, and are stood 1.2h, are obtained bacteriolyze enzymatic mixture；
4 parts of polyhexamethylene arc is taken, water is added, stirring makes polyhexamethylene arc just be completely dissolved, obtains polyhexamethylene
Remaining egg-shell meal is placed in polyhexamethylene arc water by arc water, and swaying makes it be uniformly mixed, and is stood 1.2h, is obtained polyhexamethylene
Bacteriolyze enzymatic mixture is uniformly mixed with polyhexamethylene arc mixture and stands 40min, obtains eggshell powder mixture by arc mixture；
Step 3: 10 parts of extracting honeysuckle, mashing, obtain honeysuckle slurry, honeysuckle slurry is placed in reaction kettle, honeysuckle is added
The sieving that the lemon juice of 0.2 times of amount of slurry, 0.2 times of 400 mesh of eggshell measured sieve after mixing, after carrying out pressurized treatments, is down to
Normal pressure crosses 500 mesh and sieves to obtain honeysuckle filtrate and honeysuckle filter residue；
The onion of the other half parts by weight is taken, is beaten, is obtained onion slurry, onion slurry is placed in reaction kettle, onion slurry 0.2 is added
The beer of amount after mixing, after carrying out pressurized treatments, is down to normal pressure, onion filtrate and onion filter residue is filtered to obtain, to onion again
The adsorbent of onion filtrate gross mass 10% is added in filtrate, stands 25min after stirring evenly, filters to obtain absorption filtrate, wherein inhales
Attached dose is diatomite, and the specific method of pressurized treatments is to adjust reactor pressure to be 300kPa and keep 40min, increases reaction kettle
Interior pressure keeps 15min to 350kPa, and the pressure in increase reaction kettle to 380kPa keeps 8min；
Step 4: being mixed by bottoms, honeysuckle filter residue, with onion filter residue, mixing slag is obtained, mixing slag gross mass is added
The water of 3 times of amounts, carries out 3 times repeatedly freezing heating treatments after being uniformly mixed, wherein freezing heating treatment is specially：First
Be placed under -25 DEG C of environment and freeze 18min, take out postposition and thaw at room temperature, after with the heating rate of 10 DEG C/min be warming up to 50
DEG C and keep 8min, continue to be warming up to 70 DEG C of holding 8min with the heating rate of 10 DEG C/min, finally with the heating of 10 DEG C/min
Rate is warming up to 100 DEG C of holding 8min, is down to room temperature, filters to obtain mixing filtrate；
Step 5: after mixing by honeysuckle filtrate, absorption filtrate and mixing filtrate, eggshell powder mixture being added, stirs
Mixing makes it be uniformly mixed, and stands 1.5h, and essential oil powder mixture is added, is uniformly mixed, and triethanolamine is added, and adjusts pH and is
5.5 to get pH be 5.5 lysozyme disinfecting liquid.
<Comparative example 1>
Do not include egg-shell meal in lysozyme disinfecting liquid raw material, i.e., by the onion essential oil in step 1, the bacteriolyze in step 2
Enzyme water, polyhexamethylene arc water, the mixing filtrate in step 3 in honeysuckle filtrate, absorption filtrate and step 4 directly mix,
It is 5.6 to get lysozyme disinfecting liquid that triethanolamine, which is added, and adjusts pH, the onion essential oil in step 1, the lysozyme in step 2
Water, polyhexamethylene arc water, the preparation method of the mixing filtrate in step 3 in honeysuckle filtrate, absorption filtrate and step 4
With embodiment 2.
<Comparative example 2>
In lysozyme disinfecting liquid raw material include honeysuckle, onion, in preparation method include step 1 and Step 3:
And step 4, step 2 and step 5 are specially：Bacteriolyze enzymatic mixture is uniformly mixed standing with polyhexamethylene arc mixture
30-40min obtains eggshell powder mixture, and triethanolamine is added, and adjusts pH as 5.7 to get lysozyme disinfecting liquid, wherein lysozyme
The preparation method of mixture and polyhexamethylene arc is the same as same embodiment 2.
<Comparative example 3>
Step 3: step 4 is specially：The onion of 8 parts of extracting honeysuckle, bottoms and the other half parts by weight is beaten, will
Mixing slurry is warming up to 100 DEG C of holding 30min, is down to room temperature, filters to obtain mixing filtrate；
Step 5 is specially：Eggshell powder mixture is added into mixing filtrate, stirring makes it be uniformly mixed, and stands 1-
1.5h is added essential oil powder mixture, is uniformly mixed to get lysozyme disinfecting liquid.
Remaining step is the same as embodiment 2, wherein it is 5.6 to adjust pH.
Lysozyme disinfecting liquid clinical application prepared by embodiment 1-3：It can be used for hygienic skin of hand disinfection, surgery skin of hand disappears
Poison, the skin degerming processing before the wrapping of surface of a wound skin；
Application method：Spray will be made in the packing of above-mentioned lysozyme disinfecting liquid and spray bottle, being rocked before use makes in spray bottle admittedly
Liquid is uniformly mixed, sprinkling, wherein hygienic skin of hand disinfection, in right amount in the palm of the hand, both hands, which are mutually rubbed with the hands, makes each portion of coating for sprinkling
, clear water is rinsed well after 1min；Surgery skin of hand sterilizes, before surgical hand, spray appropriate this product in both hands, forearm and
1/3 skin under upper arm, mutually rubbing with the hands makes each position of coating, and clear water is rinsed well after acting on 5min；Non- surface of a wound skin degerming
Processing, simply ventilative wrapping after spraying appropriate this product at the surface of a wound.
Points for attention：Disinfectant for external use, the term of validity 18 months.
1, multiple skin irritation test
The White male rabbit 4 for selecting regular grade white New Zealand kind adult, health, skin zero damage, is randomly divided into
Two groups, every group 2, family's rabbit back backbone diamond wool is shaved off before experiment, formed at the blocky shaving of about 2cm*3cm square, point
Lysozyme disinfecting liquid made from embodiment 2 and distilled water correspondence are not applied at three groups of rabbit bulk shavings, each each family
It is about 0.4ml that rabbit, which smears volume, is then covered with two layers of gauze and one layer of glassine paper, then be subject to nonirritant adhesive plaster and bandage
It is fixed, smeared every 4h primary, continuous 5 days, warm water wash residue used before smearing every time, recorded after smearing 1h every time as a result,
It scores according to table 1, the results are shown in Table 2, wherein：
The sum of every animal average integral=this animal erythema or oedema integral/number of days in every group
1 skin wound repair of table scores
2 skin irritation test result of table
As shown in Table 2, the lysozyme disinfecting liquid that prepared by embodiment 2 is to the irritation very little of skin, with control group distilled water
No significant difference.
2, multiple pigmenting of skin experiment
Warm water wash residue is used before being smeared every time in multiple skin irritation test, and records skin after residue cleaning
Color finds that lysozyme disinfecting liquid to the basic non-coloring of skin, easily elutes, with control group distilled water no significant difference.
3, skin surface disinfection experiment
Respectively Example 2, comparative example 1, comparative example 2, comparative example 3 prepare lysozyme disinfecting liquid, to skin surface into
Row disinfection, before surveying disinfection, after disinfection after 4min, disinfection after 30min, disinfection 2h total plate count, as shown in table 3：
Table 3
As shown in Table 3：
The first, when the initial 4min of disinfecting power of lysozyme disinfecting liquid prepared by embodiment 2 is detected, with comparative example 1 without bright
Significant difference is different, and when all having higher sterilizing ability, but being detected after 30min after disinfection, the clump count of comparative example 1 is in increase trend,
And the bacterium colony of embodiment 2 has slight decrease trend, meanwhile, when being detected after 2h after disinfection, the clump count of comparative example 1 is significantly greater than in fact
The clump count for applying example 2 illustrates that the sterilizing ability persistence of the lysozyme disinfecting liquid of embodiment 2 is strong, mainly due to egg-shell meal
Addition, since eggshell itself belongs to the porous material with micropore, by egg-shell meal respectively with onion essential oil, lysozyme water,
And the mixing of polyhexamethylene arc water is stood, and can be partially absorbed and be stored the effective Fungicidal substance in part, slow down releasing for Fungicidal substance
Dynamics is put, and then enhances the sterilization persistence of lysozyme disinfecting liquid, egg-shell meal inhales the adsorption capacity of aqueous solution with essential oil
Attached ability is different, and then further be staggered extends the sterilization persistence ability of lysozyme disinfecting liquid, and the setting of egg-shell meal is same
Sample can effectively improve attachment of the sterilization component in egg-shell meal and cocoanut active charcoal circumferential direction of lysozyme anti-virus solution, and then into one
Step extends the action time of thimerosal on the skin；
The second, when the initial 4min of sterilizing ability of lysozyme disinfecting liquid prepared by embodiment 2 is detected, it is more than comparative example 3
Sterilizing ability, mainly due to the sieving for adding lemon juice and 400 mesh of eggshell sieve in honeysuckle, and in mixing last handling process
Using gradient pressurized treatment so that honeysuckle cell damage is more effective, and Fungicidal substance obtains extraction as much as possible, in onion
Gradient pressurized treatment is used after adding beer, increases onion cells breakage efficiency, can effectively reduce onion penetrating odor, make
Fungicidal substance extraction as much as possible, while the use of adsorbent further decrease the sense of discomfort that onion taste brings user,
The repetitious freezing heating treatment of freezing of mixing slag, same reservation bottoms, honeysuckle filter residue and ocean as much as possible
Fungicidal substance in green onion filter residue there is apparent enhancing inhibiting effect equally to disappear gram-negative bacteria, pathogen, shigella dysenteriae
It detects after 30min after poison, and is detected after 2h after disinfection, the clump count of comparative example 3 first reduces is slowly increased trend afterwards, illustrates to compare
Egg-shell meal is applied in example 3 has humidification for the sterilization persistence of thimerosal；
When the initial 4min of sterilizing ability of lysozyme disinfecting liquid prepared by third, comparative example 3 is detected, it is more than comparative example 2
Sterilizing ability illustrates that the addition of honeysuckle and onion enhances tool facilitation for lysozyme disinfecting liquid sterilizing ability, while right
When ratio 2 initial 4min detections, sterilizing rate (4min clump counts after clump count/disinfection before disinfection) is more than 85%, wherein lysozyme
The alkaline enzyme that polysaccharide is sticked in pathogenic bacteria can be effectively hydrolyzed, by destroying -acetylmuramic acid and N- acetylaminos in cell wall
β-Isosorbide-5-Nitrae glycosidic bond between glucose makes the insoluble glutinous polysaccharide of cell wall resolve into soluble glycopeptide, leads to cell wall rupture content
Object escapes and bacterolysis is made to reach bactericidal effect, and polyhexamethylene arc can generate ionization, hydrophilic base portion in aqueous solution
Dividing has strong electropositive, and absorption is usually in that electronegative all kinds of bacteriums, virus inhibit film lactones plastid into cell membrane
Synthesis, causes thalline apoptosis, reaches effective sterilization, and the entirety that cooperation honeysuckle, onion further enhance lysozyme disinfecting liquid disappears
Toxic effect fruit.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and shown here as with description embodiment.
Claims (7)
1. lysozyme disinfecting liquid, which is characterized in that include the raw material of following parts by weight：4-5 parts of lysozyme, polyhexamethylene arc 1-
5-10 parts of 4 parts, 3-4 parts of egg-shell meal, 6-10 parts of honeysuckle and onion.
2. lysozyme disinfecting liquid as described in claim 1, which is characterized in that further include：Triethanolamine, triethanolamine adjust molten
The pH of bacterium enzyme disinfecting liquid is 5.0-6.0.
3. the preparation method of lysozyme disinfecting liquid as described in claim 1, which is characterized in that include the following steps：
Step 1: component meter by weight, takes 5-10 parts of onion, the onion of half parts by weight to obtain onion precision with steam distillation
Oil and bottoms；
It takes eggshell to crushed 400 mesh sieve, removes sieving, 400 mesh on-the-sieve materials continued 200 mesh sieve, and obtained egg-shell meal, take eggshell
3-4 parts of powder, the egg-shell meal of a quarter parts by weight is placed in onion essential oil, and stirring makes it be uniformly mixed, and is stood 3-4h, is obtained essential oil
Powder mixture；
Step 2: taking 4-5 parts of lysozyme, water is added, stirring makes lysozyme just be completely dissolved, obtains lysozyme water, take egg-shell meal
2/4ths parts by weight are placed in lysozyme water, and swaying makes it be uniformly mixed, and are stood 1-1.2h, are obtained bacteriolyze enzymatic mixture；
1-4 parts of polyhexamethylene arc is taken, water is added, stirring makes polyhexamethylene arc just be completely dissolved, obtains polyhexamethylene arc
Remaining egg-shell meal is placed in polyhexamethylene arc water by water, and swaying makes it be uniformly mixed, and is stood 1-1.2h, is obtained polyhexamethylene
Bacteriolyze enzymatic mixture is uniformly mixed with polyhexamethylene arc mixture and stands 30-40min by arc mixture, obtains egg-shell meal mixing
Object；
Step 3: 6-10 parts of extracting honeysuckle, mashing, obtain honeysuckle slurry, honeysuckle slurry is placed in reaction kettle, honeysuckle slurry is added
The sieving that the lemon juice of 0.2 times of amount, 0.2 times of 400 mesh of eggshell measured sieve after mixing, after carrying out pressurized treatments, is down to often
Pressure crosses 500 mesh and sieves to obtain honeysuckle filtrate and honeysuckle filter residue；
The onion of the other half parts by weight is taken, is beaten, is obtained onion slurry, onion slurry is placed in reaction kettle, 0.2 times of amount of onion slurry is added
Beer after mixing, carry out pressurized treatments after, be down to normal pressure, onion filtrate and onion filter residue filtered to obtain, to onion filtrate
The adsorbent of onion filtrate gross mass 10% is added, stands 20-25min after stirring evenly, filters to obtain absorption filtrate；
Step 4: being mixed by bottoms, honeysuckle filter residue, with onion filter residue, mixing slag is obtained, mixing slag gross mass 2-3 is added
The water of amount again, carries out 3 times repeatedly freezing heating treatments after being uniformly mixed, wherein freezing heating treatment is specially：First set
Freeze 15-18min under -25 DEG C of environment, take out postposition and thaw at room temperature, after with the heating rate of 10 DEG C/min be warming up to 50
DEG C and keep 6-8min, continue to be warming up to 70 DEG C of holding 6-8min with the heating rate of 10 DEG C/min, finally with 10 DEG C/min's
Heating rate is warming up to 100 DEG C of holding 6-8min, is down to room temperature, filters to obtain mixing filtrate；
Step 5: after mixing by honeysuckle filtrate, absorption filtrate and mixing filtrate, eggshell powder mixture is added, stirring makes
It is uniformly mixed, and stands 1-1.5h, and essential oil powder mixture is added, is uniformly mixed to get lysozyme disinfecting liquid.
4. the preparation method of lysozyme disinfecting liquid as claimed in claim 2, which is characterized in that adsorbent is diatom in step 3
Soil.
5. the preparation method of lysozyme disinfecting liquid as claimed in claim 3, which is characterized in that it is mixed that essential oil powder is added in step 5
Object is closed, triethanolamine is added after being uniformly mixed, adjusting pH is 5.0-6.0, obtains the lysozyme disinfecting liquid that pH is 5.0-6.0.
6. the preparation method of lysozyme disinfecting liquid as claimed in claim 3, which is characterized in that in step 3, pressurized treatments
Specific method is to adjust reactor pressure to be 300kPa and keep 30-40min, and the pressure in increase reaction kettle to 350kPa is kept
10-15min, increase reaction kettle in pressure to 380kPa keep 6-8min.
7. application of the lysozyme disinfecting liquid as claimed in claim 1 or 2 in skin degerming.
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