CN103356999A - Novel marine organism washing-free disinfection solution and its application - Google Patents
Novel marine organism washing-free disinfection solution and its application Download PDFInfo
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Abstract
Belonging to the technical field of disinfection, the invention discloses a novel marine organism washing-free disinfection solution. In view of the disadvantages of existing disinfection technologies, especially preoperative disinfection technologies, the invention provides the novel marine organism washing-free disinfection solution, which comprises the following active ingredients by mass: 0.5%-2% of chitosan, 1-4% of chitosan oligosaccharide, 2%-8% of baicalin, 0.5%-2% of prebiotics, 0.5%-1.5% of polyhexamethylene guanidine, 2%-5% of lysozyme and 1.5%-5% of a Polygonum cuspidatum extract. The disinfection effect is good. The disinfection solution disclosed by the invention can be used for sanitation disinfection or the hand disinfection or skin disinfection in preoperative disinfection. With excellent sterilization effect and lasting effect, the novel marine organism washing-free disinfection solution has very good application prospects and economic benefits.
Description
Technical field
The invention belongs to the disinfectant technical field, be specifically related to the biological disposable disinfectant solution of a kind of novel sea.
Background technology
In the clinical treatment nursery work, medical personnel must strictly observe the sterile working when carrying out operations.Especially more frequent surgery contact patient, the probability that infection occurs is also relatively larger.Therefore, clinical various treatment, diagnostic procedure all need skin degerming; Sterilization is the important step of infection control, and the success or not of sterilization directly affects therapeutic quality.Although have some disinfectant solution virus killing effects can reach requirement in the various skin antiseptics, action time is shorter, fungicidal spectrum is narrow, and skin irritation is large, and discomfort is being widely used for Clinical Surgery.Select the skin antiseptic of high-efficiency low-toxicity then to become whether successful key factor of sterilization.
Clinical common skin antiseptic mainly contains ethanol, iodine and povidone iodine, beneficial rhzomorph, quaternary ammonium compound, triclosan etc.Wherein 1) ethanol has reliable bactericidal action, wherein the alcoholic solution bactericidal effect of 60.0%-80.0% valid density is best, is clinical the most frequently used concentration, but because its fungicidal spectrum is narrower, killing effect is relatively poor, uses separately the alcoholic solution sterilization can not satisfy the needs of clinical disinfection.2) iodine and Iodic agent are by the skin antiseptic of wide clinical application.Clinical Iodic agent commonly used mainly is iodine tincture, iodine water and povidone iodine.Povidone iodine is the current topmost iodine skin antiseptic that contains, and is the indefinite form complex that iodine and surfactant are combined into.Its contained surface active ingredient can change solution to the wettability of object, can form one deck sterilization thin film as thin as a wafer at skin surface.Have the iodine of assistance and penetrate the Organic substance effect, and the energy chyle fat, slowly lasting release available iodine is strengthened the bactericidal action of iodine.
Chlorhexidine belongs to the biguanides antibacterial, has the characteristics such as toxicity is low, zest is little, has a broad antifungal spectrum, mainly is used as the antimicrobial treatment of skin and mucosa.Itself is insoluble in water chlorhexidine, but the form of gluconic acid is water miscible.Antibacterial activity is to stick and destroy the cell serous coat, causes the cellular content precipitation.At once the antibacterial activity of chlorhexidine is slower than ethanol.It has good anti-gram positive bacteria effect, and is little to the Mycobacterium effect a little less than the effect to gram-negative bacteria and fungus, invalid to the brood cell.In vitro tests shows effective to virus such as herpesvirus, HIV, cytomegalovirus, influenza virus and RSV that film is arranged, but obviously lower to virus such as rotavirus, enterovirus and adenovirus effectiveness without film.
Quaternary ammonium salt is large class formation chemical compound complicated and changeable.Wherein alkyl Benzalkonii Chloridum (benzalkonium bromide) is widely used clinical.Other chemical compounds as disinfectant comprise Benzalkonii Chloridum, benzalkonium bromide.Once be widely used in the every field such as health care.Although the quaternary ammonium salt antibacterial bacteriostasis such as discovered in recent years benzalkonium bromide are very strong, fungicidal spectrum is narrower: the effect to gram-negative bacterias such as Pseudomonas aeruginosas is relatively poor, and is then substantially invalid to mycobacterium tuberculosis and bacterial spore; Only but the deactivation lipophilic is viral, and relatively poor to the hydrophilic viruses effect.
Triclosan is a kind of high-efficiency broad spectrum antibacterial.In the later development sixties in 20th century, be widely used in the consumer goodss such as soap, toothpaste, cleansing milk, handwashing liquid for medical personnel and public place.Antibiotic concentration is 0.2%-2.0%.The antibacterial activity of triclosan is to enter bacterial cell and affect cell serous coat and RNA, fatty acid and protein synthesis.Triclosan has certain antimicrobial spectrum, but is partial to antibacterial.Triclosan is better than gram negative bacilli to the effect of gram positive bacteria (comprising M RSA), particularly Pseudomonas aeruginosa.Triclosan has certain activity to Mycobacterium and Candida, but to the activity of filament fungus a little less than.Studies show that in a large number, lower than beneficial rhzomorph, povidone iodine and alcohol product with the wash one's hands reduction number of rear bacterium colony of triclosan.
The reappraise safety of triclosan of U.S. FDA in 2011 and EPA comes from the product that contains triclosan in case react with chloride tap water, can form the material of a kind of being called as " chloroform ", i.e. chloroform.The chloroform animal experiment finds to have slight teratogenecity to heart and liver injury, can induce white mice generation hepatocarcinoma, but there is no human carcinogen's research data.For the sake of assurance, IARC and the U.S. all classify chloroform as the suspect carcinogen that human body is had potential threat.In clinical use, because abuse of antibiotics is carried out natural selection to the variation antibacterial, thereby produced " superbacteria ", it can make malignant bacteria incomparably powerful, resists all antibiotic.
Therefore; exploitation safety, wide spectrum, efficient, working concentration is low, environmental friendliness, user of service's skin is had the disinfectant of protection is the important of following clinical demand always; along with the increase of testing microorganism and disinfectant unsafe factor; requirement for experiment condition is increasingly strict, causes the new disinfectant development difficulty large, expensive many.Also be no lack of on the market the Chinese medicine extract of containing gel, adopt several kinds of Chinese medicinal materials composite more, the effect complicated, mechanism is indefinite.The invention provides a kind of definite ingredients, have the plant dry-cleaning disinfecting gel of excellent antibacterial effect.
Chitosan (chitosan) is to be obtained through deacetylation by the chitin (chitin) that nature extensively exists, chemical name is Chitosan (1-4)-2-amino-B-D glucose, from 1859, after Frenchman Rouget at first obtains chitosan, biological functionality and the compatibility of this natural polymer, blood compatibility, safety, the premium properties such as microbic resolvability are by all trades and professions extensive concern, at medicine, food, chemical industry, cosmetics, water treatment, METAL EXTRACTION and recovery, the applied research of the numerous areas such as biochemistry and biomedical engineering has been obtained major progress.The bacteriostatic activity chitosan of chitosan in the weak acid solvent, be easy to the dissolving, it is worthy of note especially the dissolving after solution in contain amino (NH2+), these amino are by coming anti-bacteria in conjunction with negatron.The Antibacterial activity of chitosan makes it have a wide range of applications in fields such as medicine, weaving and food.Chitosan and derivant thereof have preferably antibacterial activity, can suppress the growth and breeding of some funguses, antibacterial and virus.Think that at present its possible mechanisms has three: one, because the polycation of chitosan is easy to the group effect of fungal cell's surface band negative charge, thereby change flowability and the permeability of pathogen cell membrane; The 2nd, disturb copying and transcribing of DNA; The 3rd, the metabolism of blocking-up pathogen.In recent years, have many researcheres to propose chitosans by inducing pathogenesis-related proteins, the modes such as accumulation secondary metabolite and signal conduction reach the viewpoint of antibiotic purpose.
Chinese patent CN1309304C discloses a kind of disinfecting gel and its preparation method and application, and it comprises staphylococcus lysozyme 0.0001-0.01wt%, lysozyme 0.01-1wt%, thickening agent 1-20wt%, emollient 0.1-15wt%, water surplus.It can be used for the cleaning and disinfection of hand skin, but this disinfecting gel in use long-term killing effect is not good.
Chinese patent application CN1498538A discloses a kind of marine organism sterilizing agent take chitin, alginic acid element, agaroidin gel sugar, DP300 DP300, cedar wood alcohol as effective ingredient, this disinfectant is non-stimulated, abnormal smells from the patient delicate fragrance, but its same existence is defective of virus killing poor effect for a long time, is difficult to adapt to the needs of prolonged operations.
Therefore, develop a kind of skin irritation little, the Novel free decontamination venom of sterilization spectrum width and long action time is a job that shoulders heavy responsibilities to vast medical matters researcher.
Summary of the invention
The invention provides the biological disposable disinfectant solution of a kind of novel sea, the biological disposable disinfectant solution of novel sea of the present invention can be used for hands sterilization or the skin degerming in disinfecting or the surgery preoperative disinfection, its bactericidal effect is excellent, and effect is lasting, has good application prospect and economic benefit.
The present invention adopts following technical scheme to realize:
The biological disposable disinfectant solution of a kind of novel sea of the present invention, its active component is comprised of chitosan, oligochitosan, baicalin, beneficial rhzomorph, polyhexamethylene guanidine, lysozyme and Rhizoma Polygoni Cuspidati extract.
Wherein:
Chitosan is by β-(1,4) the glucosamine macromole natural polymer of glycosidic bond connection, it is the unique polysaccharide with the positive charge effect of occurring in nature, chitosan is by the entrained negative charge interaction of self entrained positive charge and microbial cell, destroy the bacterial cell wall construction, cause cell component to leak and play antibacterial action; To there not being the microorganism of cell membrane; similar flocculation occurs by penetrating in the cell with biomacromolecule with anion; upset the normal physiological function of cell; the blocking dna biosynthesis; thereby the Growth and Reproduction of anti-bacteria does not produce drug resistance, significantly improves Effects on local immunological functions, improves mucosa hyperemia, protects mucosa, promotes epithelial cell growth.
Oligochitosan also is Chitosan oligosaccharide, also claim chitin oligosaccharide, formal name used at school β-Isosorbide-5-Nitrae-oligosaccharide-glucamine, being a kind of brand-new product that chitosan is obtained through special biological enzyme technology processing, is the low molecular weight product that water solublity is better, function is large, biological activity is high.It has the unexistent higher solubility of chitosan and easily by the function of many uniquenesses such as organism absorption, and it act as 14 times of chitosan.Prevention or treatment are by bacterial Animal diseases such as staphylococcus aureus, Escherichia coli, Actinobacillus, streptococcus mutanses.But the disease resistance of inducing plant produces immunity and killing action to multiple fungus, antibacterial and virus.Antioxidation: can eliminate the human body oxygen-derived free radicals, the activation equipment somatic cell, slow down aging suppresses the skin surface harmful bacteria and grows, and performance of keeping humidity is excellent.
Baicalin (Baicalin) is to extract a kind of flavone compound separate from scutellariae,radix, has significant biological activity, has antibacterial, diuresis, antiinflammatory, resistance attitude and spasmolysis, and has the stronger physiological potencies such as antitumor response.Occupied critical role at clinical medicine.Baicalin can also absorb ultraviolet, removes oxygen-derived free radicals, and therefore generation that again can check melanin both can be used for medicine, also can be used for cosmetics, was a kind of good functional cosmetics raw material.Be one of Radix Scutellariae antimicrobial component, contain the clinical trachoma that is used for the treatment of of collyrium of 3% baicalin, medicament benemicin is similar preferably to treatment trachoma for curative effect.
The benefit rhzomorph is take Caulis Sacchari sinensis as primary raw material, is transformed into oligofructose through the catalysis of a series of enzymes, is equipped with the Semen Cassiae elite again.The benefit rhzomorph is the nutrient source of bacillus bifidus, with the breeding doubly of bacillus bifidus 10-100 in the rear intestinal.Bacillus bifidus is the most bellicose beneficial bacteria of inside of human body, and it the means such as engulfs by squeezing, and the harmful bacteria of inside of human body is drained or eliminated.Oligofructose is called as " beneficial bacteria factor " because of the effectiveness that bacillus bifidus is bred rapidly.The benefit rhzomorph is the low fructose series of products with more outstanding a kind of.It not only can suppress harmful bacteria growth in the intestinal, promotes the rapid breeding of probiotics, improves intestine microenvironment, and can set up the intestinal biological barrier, stops the pathogenic bacterium invasion, prevents intractable diarrhea that the function of intestinal canal disorder causes and constipation etc.
Polyhexamethylene guanidine has the sterilization wide spectrum; Valid density is low; Speed of action is fast; Stable in properties; Premium properties soluble in water; Can use at normal temperatures; For a long time antibacterial, have no side effect; Non-corrosiveness; Colourless, odorless; Nontoxic; Do not fire, not quick-fried, use safety; Moderate; Convenient transportation can be described as best antibacterial.In fact, this product is a kind of environment-friendly multipurpose new polymers, in industrial, agriculture, medical and daily life extremely widely purposes is arranged.It is polyguanidine family macromolecule polymer, can produce ionization in aqueous solution, and its hydrophilic group partly contains strong electropositive, absorption is electronegative each bacterioid, virus usually, enters cell membrane, suppresses film lactone plastid synthetic, cause the thalline apoptosis, reach best bactericidal effect.
Lysozyme (lysozyme) claims again muramidase (muramidase) or N-acetylmuramide lycanohydrlase (N-acetylmuramide glycanohydrlase), is a kind of alkaline enzyme that can be hydrolyzed mucopolysaccharide in the pathogenic bacterium.Main-acetylmuramic acid and the β between the NAG-Isosorbide-5-Nitrae glycosidic bond that passes through to destroy in the cell wall makes the insoluble mucopolysaccharide of cell wall resolve into the solubility glycopeptide, causes the effusion of cell wall rupture content and makes bacterolysis.Lysozyme also can with the direct combination of electronegative virus protein, form double salt with DNA, RNA, apoprotein, make virally inactivated.Therefore, this enzyme has the effects such as antibiotic, antiinflammatory, antiviral.Medically can be used as a kind of natural infection material with bactericidal action, have antibiotic, antiviral, hemostasis, reducing swelling and alleviating pain and accelerate the effect such as organized renewing function.Clinical in chronic rhinitis, acute and chronic pharyngitis, oral ulcer, chickenpox, herpes zoster and verruca plana etc.Also can share the various antibacterials for the treatment of and viral infection with antibacterials.Oral all effective with intramuscular injection.
Emodin in the Rhizoma Polygoni Cuspidati, 7-acetyl group-2-methoxyl group-6-methyl-8-hydroxyl-1,4-naphthoquinone, resveratrol all have antibacterial activity.The resveratrol In vitro Bactericidal Experiments shows: it has powerful anti-microbial property to trichophyton rubrum, the trichophyton interdigitalis that causes stubborn dermatitis, pompholyx shape tinea alba, and to bacillus subtilis, Resina garciniae eight repeatedly bacterium etc. stronger bactericidal action is arranged.Emodin, PD, emodin-8-glycoside have inhibitory action to staphylococcus aureus, hepatitis diplococcus.A kind of Flavonoid substances that contains in the Rhizoma Polygoni Cuspidati has inhibitory action to staphylococcus aureus, Staphylococcus albus, Bacillus proteus.But 10% Rhizoma Polygoni Cuspidati decocting liquid all has inhibitory action to herpes simplex virus, the influenza Asia first type capital 68-1 of section virus and dust II type virus (ECHO11).3% decocting liquid to No. 479 adenovirus type IIIs, No. 72 poliomyelitis II types, No. 44 dusts can 9 types, COxsackie A9 type and B5 type, encephalitis B (Jing Wei grinds the I strain), No. 140 7 kinds of representative Strain such as herpes simplex all have stronger inhibitory action.Rhizoma Polygoni Cuspidati monomer I and II can make the hepatitis B antigen titre reduce by 8 times.For clearer expression Rhizoma Polygoni Cuspidati extract of the present invention, the applicant provides a kind of preparation method for preparing above-mentioned Rhizoma Polygoni Cuspidati extract, its step comprises: get dry Rhizoma Polygoni Cuspidati, pulverize, sampling 10g is contained in the beaker, adds to extract solvent 120mL, with sodium hydroxide solution or hydrochloric acid solution the pH value of lixiviating solution is transferred to designated value, soak 16～24h.Solution colour becomes yellowish-brown or 4 layers of filtered through gauze of brown rear usefulness, gets 55mL filtrate and be distilled to 5mL with rotary evaporator under 50 ℃.Cold preservation, for subsequent use.
The present invention makes disinfectant solution with above-mentioned active component composition according to optimal proportion, through test of many times and clinical verification, can either broad-spectrum high efficacy kill common pathogenic flora, reduce the clinical infection probability has good histocompatibility simultaneously, and skin irritation is little.The multi-medicament component is share, and has significant synergism aspect the common pathogen killing, and stops sticking, breed of pathogenic bacterium, has also avoided using the drug resistance, superinfection, anaphylaxis of antibiotic initiation and to the drawbacks such as toxic and side effects of body.
The inventor explores constituent content in the above-mentioned disinfectant solution as evaluation criterion the killing effects of pathogenic bacterium take above-mentioned disinfectant solution, the inventor finds that component weight percentage is in above-mentioned disinfectant solution: chitosan 0.5%-2%, oligochitosan 1-4%, baicalin 2%-8%, benefit rhzomorph 0.5%～2%, polyhexamethylene guanidine are 0.5%～1.5%, lysozyme 2%-5%, Rhizoma Polygoni Cuspidati extract is 1.5%-5%, surplus is water, and described disinfectant solution is better to the killing effect of pathogenic bacterium.Wherein work as disinfectant solution and contain chitosan 1.5%, oligochitosan 3%, baicalin 5%, beneficial rhzomorph 1.4%, polyhexamethylene guanidine are 1%, lysozyme 3%, and when Rhizoma Polygoni Cuspidati extract was 3.5%, its killing effect to pathogenic bacterium was best.
Disinfectant solution of the present invention can also contain wetting agent and pH regulator except containing active component.All can be used for disinfectant solution of the present invention about wetting agent and pH regulator in the prior art.Wherein said wetting agent is preferably glycerol.Described pH regulator is preferably triethanolamine, triisopropanolamine, diisopropanolamine (DIPA) or its combination.The pH of described disinfectant solution is preferably 5.0-6.5.
The biological disposable disinfectant solution of novel sea of the present invention can be widely used in the skin degerming field, and its mechanism of action is:
A) chitosan is macromolecule polyalcohol, and the strand disperse is stretched, and is great swelling state, and has viscosity; Polyhexamethylene guanidine has stronger biocidal property and film property.Disinfectant solution of the present invention can form the gelatinous mass of the thickness of clarification, and gel is attached to skin surface and forms protecting film, and sterilization face is isolated from the outside, and avoids the secondary cross infection.
B) this gel is subacidity, be not subjected to microbiological effect, particularly multiple bacteriostatic active ingredients coupling in the disinfectant solution of the present invention, not only can enlarge the anti-pathogenic bacterium spectrum of disinfectant solution, and can aspect antibacterial activity, have significant collaborative reinforced effects, thereby suppress the pathogen breeding, strengthen diseased region to resistivity and the immunocompetence of pathogenic bacteria, virus.
C) characteristics of the equal tool water-soluble base of chitosan in performance subjective antisepsis activity, can slowly discharge parcel beneficial rhzomorph, baicalin and Rhizoma Polygoni Cuspidati extract wherein, prolong the virus killing effect of compositions.
Based on this, the invention provides a kind of purposes of above-mentioned disinfectant solution, namely above-mentioned disinfectant solution is used in hands sterilization or skin degerming, and wherein said hands sterilization or skin degerming can be disinfecting or surgery preoperative disinfection.The embodiment of the invention 7 shows that disinfectant solution of the present invention all has stronger killing action to most pathogenic bacterium certificate.The embodiment of the invention 8 shows that disinfecting gel of the present invention can effectively kill staphylococcus aureus (Staphyloccocus aureus), pseudomonas aeruginosa (Pseudomonas aeruginosa), escherichia coli (Escherichia coli) and Candida albicans (Candida albicans) etc., and the sterilization 15min after and 200min after suppression ratio all reach more than 98.5%, be better than reference embodiment 1 and reference embodiment 2.On disinfecting time, the biological disposable disinfectant solution of novel sea of the present invention is more lasting action time, the bacterium colony degree of killing of 15min and 200min is more or less the same, and the bacterium colony killing rate of reference embodiment 1 and reference embodiment 2 is along with the prolongation of disinfecting time fast reducing then.The long operation preferably of the biological disposable disinfectant solution of novel sea of the present invention is used.The demonstration of the embodiment of the invention 9 experimental results, repeatedly medication is very little to skin irritation for the biological disposable disinfectant solution of novel sea of the present invention, with the matched group there was no significant difference.
The using method of the biological disposable disinfectant solution of novel sea of the present invention is: 1) surgery preoperative disinfection: after washing one's hands with soap lye by standard, flowing water washes down, dry with sterile towel, then get this product stock solution and evenly be applied to hands and forethiga one time, every hands arm is 5-10ml approximately, acts on 3 minutes both hands rubbings to doing.2) disinfecting of hands: get this product 3ml and be applied to 1 minute rubbing of hand effect to doing.3) skin health sterilization: get this product and be applied in right amount or be sprayed on skin surface to moistening, act on 3 minutes natural dryings.
When the biological disposable disinfectant solution of novel sea of the present invention is used for hands sterilization or skin degerming, compared with prior art have following technical advantage:
1) the biological disposable disinfectant solution of novel sea that makes by rational formula of the present invention is by the multiple bacteriostatic active ingredients of coupling, each component obtains remarkable synergism aspect antibacterial when significantly enlarging antimicrobial spectrum, thereby suppress the pathogen breeding, strengthen diseased region to resistivity and the immunocompetence of pathogenic bacteria, virus.
2) active component mainly comes from nature in the biological disposable disinfectant solution of novel sea of the present invention, good biocompatibility, and side effect is low, the drug resistance of having avoided using antibiotic to cause, superinfection, anaphylaxis and to the drawbacks such as toxic and side effects of body.
)When the biological disposable disinfectant solution of novel sea of the present invention was used for skin degerming, its medicine irritation was little, and bactericidal activity is lasting, can significantly improve the therapeutic effect of medical and nursing work person's compliance and medicine, greatly reduces postoperative infection.
The specific embodiment
Below further describe the present invention by the specific embodiment, but the present invention is not limited only to following examples.Within the scope of the invention or within not breaking away from content of the present invention, spirit and scope, sanitizing composition of the present invention is suitably improved, replaces the identical component of effect, will become apparent to those skilled in the art that they all are deemed to be included within the scope of the present invention.
Embodiment 1: the biological disposable disinfectant solution of novel sea of the present invention
A kind of for the biological disposable disinfectant solution of novel sea, active component comprises chitosan, oligochitosan, beneficial rhzomorph, baicalin, polyhexamethylene guanidine, lysozyme, Rhizoma Polygoni Cuspidati extract.Wherein chitosan concentration is 1%, oligochitosan concentration is 3%, beneficial rhzomorph concentration is 1.2%, Determination of baicalin is 3.7%, polyhexamethylene guanidine concentration is 1%, lysozyme concentration is 2.5%, Rhizoma Polygoni Cuspidati extract 3%.
To prepare one kilogram of gel as example, contain 10g chitosan, 30g oligochitosan, 12g benefit rhzomorph, 37g baicalin, 10g polyhexamethylene guanidine, 25g lysozyme in every kilogram of gel, the 30g Rhizoma Polygoni Cuspidati extract.Concrete preparation method is that 10g chitosan, 25g lysozyme and 30g oligochitosan are added in 846 milliliters of purified water, with high speed dispersor it is fully dissolved; Simultaneously 12g benefit rhzomorph is well-dispersed in 50 milliliters of propylene glycol, scattered beneficial rhzomorph is added in the solution of above-mentioned abundant dissolving, continue stirring and make mix homogeneously, the pH value of solution is between 5.0 to 6.0, and product becomes gel; Use at last the aqueous solution of 0.22um microporous filter membrane aseptic filtration polyhexamethylene guanidine and the aqueous solution of Rhizoma Polygoni Cuspidati extract, add in the gel according to the content in every kilogram of gel again, add at last wetting agent and pH adjusting agent and namely can be made into finished product.
The biological disposable disinfectant solution of embodiment 2-embodiment 6 novel seas of the present invention
The skin antiseptic prescription is as shown in table 1, and preparation method is with the disposable disinfectant solution preparation method of embodiment 1 marine organisms.
The percentage ratio of table 1 embodiment 2-embodiment 6 disinfectant liquid for skin forms
The In Vitro Bacteriostasis experiment of embodiment 7 sanitizing compositions of the present invention
This tests employed strain and culture medium is respectively α-hemolytic streptococcus (32209), beta hemolytic streptococcus (32210), colon bacillus (44102), proteus vulgaris (49101), pseudomonas aeruginosa (Pseudomonas aeruginosa), Candida albicans (Candida albicans) Salmonella paratyphi A (50001), shigella dysenteriae (51252), staphylococcus aureus [26111 (5)], hemophilus influenza (58529-4), nutrient agar (in be stranded pharmaceutical biological product calibrating institute); Opacity tube (Chinese bacterial turbidity standard, the biological combinations calibrating of Chinese medicine institute).
In Vitro Bacteriostasis experiment: 1. prepare the pastille agar plate.Tested material is pressed the culture medium of doubling dilution preparation different pharmaceutical concentration, heating, modulation is required pH value separately, and 121 ℃ of sterilization 30 min are chilled to 45 ℃.Pour into into aseptic flat board (plate d=65 mm, 10 ml/ plates), finally make the extension rate that contains tested material stock solution (2g/ml) in the culture medium be respectively l:2,1:4 ... 1:1024.Do simultaneously blank culture medium separately.2. inoculation.Get the bacterium in young age that reaches the third generation, be the bacterium liquid to be checked of 108 cfu/ml by standard opacity tube corrected concentrations with 0.9% sodium chloride solution respectively, be inoculated in respectively the separately pastille agar surface of different doubling dilutions, inoculate simultaneously parallel point 3 points, make contain 2 bacterium liquid at every.The liquid-drop diameter of each vaccination is 5-8 mm.After the vaccination drying, upset is dull and stereotyped cultivates 20 h observed results (doing simultaneously blank) in 36 ℃ of constant incubators.3. criterion.Minimum dilution tested material concentration on the agar plate that bacterium colony do not occur is as its minimum inhibitory concentration (MIC).
The concentration of disinfectant solution is the total concentration of chitosan in the disinfectant solution, oligochitosan, baicalin, beneficial rhzomorph, polyhexamethylene guanidine, lysozyme and Rhizoma Polygoni Cuspidati extract isoreactivity composition in this experiment.Its minimal inhibitory concentration to each pathogenic strain is as shown in table 2:
Table 2 sanitizing composition of the present invention is to the minimal inhibitory concentration of pathogenic strain
Result's demonstration, disinfectant solution of the present invention all has stronger killing action to most pathogenic bacterium certificate.
Reference embodiment 1 adopts the disinfecting gel of CN1309304C embodiment 1
Preparation method is: the content 0.01g(0.01 wt% that at first takes by weighing staphylococcus lysozyme), the content 0.3g(0.3 wt% of lysozyme), the content 3g(3wt% of hydroxy propyl cellulose), glycerol 5g(5wt%), surplus is water, and mix homogeneously can obtain required disinfecting gel.
Reference embodiment 2 adopts among the Chinese patent application CN1498538A and fills a prescription three
Its prescription consists of: chitin 3.0-6.0, and alginic acid element 2.0-5.0, agaroidin gel sugar 1.0-2.0, DP300 DP300 0.5-0.8, cedar wood alcohol 1.8-2.0, purified water adds to 100.
The Disinfection Effect of the biological disposable disinfectant solution of embodiment 8 novel seas of the present invention relatively
Use bacterium liquid to be measured evenly to be coated onto medical apparatus surface, and get one (0.05ml) bacterium liquid to be measured and put into culture medium flat plate and cultivate, coat rapidly the present invention's disinfectant solution of sterilizing on the apparatus surface.Respectively at placing flat board to cultivate from apparatus surface scraping 1 (0.05ml) behind the 15min and behind the 200min.Each bacterium liquid is cultivated respectively 48 hours respectively countings at flat board under each bacterium preference temperature.Calculating is coated with the bacterium colony killing rate behind the 15min and 200min behind the disinfectant solution.
The Disinfection Effect of table 3 sanitizing composition of the present invention relatively
Disinfecting gel free from admixture of the present invention, without particulate matter.As can be seen from Table 3, it can effectively kill staphylococcus aureus (Staphyloccocus aureus), pseudomonas aeruginosa (Pseudomonas aeruginosa), escherichia coli (Escherichia coli) and Candida albicans (Candida albicans) etc., and the sterilization 15min after and 200min after suppression ratio all reach more than 98.5%, be better than reference embodiment 1 and reference embodiment 2.On disinfecting time, the present invention's disinfectant solution of sterilizing is more lasting action time, and the bacterium colony degree of killing of 15min and 200min is more or less the same, and the bacterium colony killing rate of reference embodiment 1 and reference embodiment 2 is along with the prolongation of disinfecting time fast reducing then.The present invention's disinfectant solution long operation preferably of sterilizing is used.
The repeatedly irritation test of embodiment 9 sanitizing compositions of the present invention
Laboratory animal selects regular grade white New Zealand to plant 4 of rabbit, 24h shaves off back part of animal spinal column diamond wool before the test, shave a mao scope left and right sides Ge Yue 3cm * 3cm, the biological disposable disinfectant solution of novel sea of the present invention that during test tested material 0.5ml(is prepared by the embodiment of the invention 3) evenly be applied on the 2.5cm * 2.5cm skin of left side, the right side compares with distilled water.Smear every day once, continuously 14d.From second day, smear front unhairing every day, clean with warm water, observe dermoreaction behind the 1h and carry out skin irritation reaction scoring, after the off-test, calculate respectively 14 days total marks, 14 days every animal integral mean values, every day, every animal integral mean value and carried out the skin irritation strength grading.Repeatedly the irritation test result is as shown in table 4 for the biological disposable disinfectant solution of novel sea of the present invention.
Table 4 sanitizing composition of the present invention is the irritation test result repeatedly
Shown by table 4 experimental result, repeatedly medication is very little to skin irritation for the biological disposable disinfectant solution of novel sea of the present invention, with the matched group there was no significant difference.
Claims (10)
1. the biological disposable disinfectant solution of a novel sea, it is characterized in that: its active component is comprised of chitosan, oligochitosan, baicalin, beneficial rhzomorph, polyhexamethylene guanidine, lysozyme and Rhizoma Polygoni Cuspidati extract.
2. the biological disposable disinfectant solution of novel sea as claimed in claim 1, it is characterized in that: described each component weight percentage of disinfectant solution active component is: chitosan 0.5%-2%, oligochitosan 1-4%, baicalin 2%-8%, benefit rhzomorph 0.5%～2%, polyhexamethylene guanidine are 0.5%～1.5%, lysozyme 2%-5%, Rhizoma Polygoni Cuspidati extract are 1.5%-5%.
3. the biological disposable disinfectant solution of novel sea as claimed in claim 2, it is characterized in that: described each component weight percentage of disinfectant solution active component is: chitosan 1.5%, oligochitosan 3%, baicalin 5%, benefit rhzomorph 1.4%, polyhexamethylene guanidine are 1%, and lysozyme 3%, Rhizoma Polygoni Cuspidati extract are 3.5%.
4. such as the biological disposable disinfectant solution of the arbitrary described novel sea of claim 1-3, it is characterized in that: Rhizoma Polygoni Cuspidati extract is the compositions that comprises the Rhizoma Polygoni Cuspidati quintessence oil in the described disinfectant solution, the preparation method of described Rhizoma Polygoni Cuspidati extract comprises the steps: to get dry Rhizoma Polygoni Cuspidati, pulverize, sampling 10g is contained in the beaker, adds to extract solvent 120mL, with sodium hydroxide solution or hydrochloric acid solution the pH value of lixiviating solution is transferred to designated value, soak 16～24h; Solution colour becomes yellowish-brown or 4 layers of filtered through gauze of brown rear usefulness, and get 55mL filtrate and under 50 ℃, be distilled to 5mL with rotary evaporator, cold preservation, for subsequent use.
5. the biological disposable disinfectant solution of novel sea as claimed in claim 4, it is characterized in that: Rhizoma Polygoni Cuspidati extract is resveratrol in the described disinfectant solution.
6. such as the biological disposable disinfectant solution of the arbitrary described novel sea of claim 1-3, it is characterized in that: described disinfectant solution contains wetting agent and pH regulator.
7. the biological disposable disinfectant solution of novel sea as claimed in claim 6, it is characterized in that: wetting agent is glycerol in the described disinfectant solution, described thickening agent is carbomer, and described pH regulator is one or more the combination in triethanolamine, triisopropanolamine, the diisopropanolamine (DIPA).
8. such as the biological disposable disinfectant solution of the arbitrary described novel sea of claim 1-3, it is characterized in that: the pH of described disinfectant solution is 5.0-6.5.
9. the biological disposable disinfectant solution of the arbitrary described novel sea of claim 1-3 is used in hands sterilization or skin degerming.
10. application as claimed in claim 9 is characterized in that: described hands sterilization or skin degerming are disinfecting or surgery preoperative disinfection.
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| CN108324933A (en) * | 2018-04-08 | 2018-07-27 | 山东鲁北药业有限公司 | Lysozyme disinfecting liquid and its preparation method and application |
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| CN103356999B (en) | 2014-12-17 |
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Inventor after: Zhang Kun Inventor after: Jiao Nianqiang Inventor after: Liu Zhongwei Inventor after: Du Guangqing Inventor before: Jiao Nianqiang Inventor before: Liu Zhongwei Inventor before: Du Guangqing |
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