CN103356999B - Novel marine organism washing-free disinfection solution and its application - Google Patents
Novel marine organism washing-free disinfection solution and its application Download PDFInfo
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Abstract
Belonging to the technical field of disinfection, the invention discloses a novel marine organism washing-free disinfection solution. In view of the disadvantages of existing disinfection technologies, especially preoperative disinfection technologies, the invention provides the novel marine organism washing-free disinfection solution, which comprises the following active ingredients by mass: 0.5%-2% of chitosan, 1-4% of chitosan oligosaccharide, 2%-8% of baicalin, 0.5%-2% of prebiotics, 0.5%-1.5% of polyhexamethylene guanidine, 2%-5% of lysozyme and 1.5%-5% of a Polygonum cuspidatum extract. The disinfection effect is good. The disinfection solution disclosed by the invention can be used for sanitation disinfection or the hand disinfection or skin disinfection in preoperative disinfection. With excellent sterilization effect and lasting effect, the novel marine organism washing-free disinfection solution has very good application prospects and economic benefits.
Description
Technical field
The invention belongs to disinfectant technical field, be specifically related to the biological disposable disinfectant solution of a kind of novel sea.
Background technology
In clinical treatment nursery work, medical personnel must strictly observe sterile working in the time carrying out operations.Especially more frequent surgery contact patient, the probability that infection occurs is also relatively larger.Therefore, clinical various treatment, diagnostic procedure all need skin degerming; Sterilization is the important step of infection control, and the success or not of sterilization directly affects therapeutic quality.Although have some disinfectant solution virus killing effects can reach requirement in various skin antiseptics, action time is shorter, fungicidal spectrum is narrow, and skin irritation is large, and discomfort is being widely used for Clinical Surgery.Select the skin antiseptic of high-efficiency low-toxicity to become whether successfully key factor of sterilization.
Clinical common skin antiseptic mainly contains ethanol, iodine and povidone iodine, beneficial rhzomorph, quaternary ammonium compound, triclosan etc.Wherein 1) ethanol has reliable bactericidal action, wherein the alcoholic solution bactericidal effect of 60.0%-80.0% valid density is best, is clinical the most frequently used concentration, but because its fungicidal spectrum is narrower, killing effect is poor, uses separately alcoholic solution sterilization can not meet the needs of clinical disinfection.2) iodine and Iodic agent are by the skin antiseptic of wide clinical application.Clinical conventional Iodic agent is mainly iodine tincture, iodine water and povidone iodine.Povidone iodine is current topmost containing iodine skin antiseptic, is the indefinite form complex that iodine and surfactant are combined into.Its contained surface active ingredient can change the wettability of solution to object, can on skin surface, form one deck sterilization thin film as thin as a wafer.There is the iodine of assistance and penetrate Organic substance effect, and energy chyle fat, slowly lasting release available iodine, strengthens the bactericidal action of iodine.
Chlorhexidine belongs to biguanides antibacterial, has the features such as toxicity is low, zest is little, has a broad antifungal spectrum, is mainly used as the antimicrobial treatment of skin and mucosa.Itself is insoluble in water chlorhexidine, but the form of gluconic acid is water miscible.Antibacterial activity is stick and destroy cell serous coat, causes cellular content precipitation.At once the antibacterial activity of chlorhexidine is slower than ethanol.It has good anti-gram positive bacteria effect, a little less than the effect of gram-negative bacteria and fungus, little to Mycobacterium effect, invalid to brood cell.In vitro tests shows there being the virus of film as effective in herpesvirus, HIV, cytomegalovirus, influenza virus and RSV, but obviously as lower in rotavirus, enterovirus and adenovirus effectiveness to the virus without film.
Quaternary ammonium salt is large class formation compound complicated and changeable.Wherein alkyl Benzalkonii Chloridum (benzalkonium bromide) is widely used clinical.Other compounds as disinfectant comprise Benzalkonii Chloridum, benzalkonium bromide.Once be widely used in the every field such as health care.Although the quaternary ammonium salt antibacterial bacteriostasis such as discovered in recent years benzalkonium bromide are very strong, fungicidal spectrum is narrower: the effect to gram-negative bacterias such as Pseudomonas aeruginosas is poor, substantially invalid to mycobacterium tuberculosis and bacterial spore; Only can deactivation lipophilic virus, and poor to hydrophilic viruses effect.
Triclosan is a kind of high-efficiency broad spectrum antibacterial.In the later development sixties in 20th century, be widely used in the consumer goodss such as soap, toothpaste, cleansing milk, handwashing liquid for medical personnel and public place.Antibacterial concentration is 0.2%-2.0%.The antibacterial activity of triclosan is enter bacterial cell and affect cell serous coat and RNA, fatty acid and protein synthesis.Triclosan has certain antimicrobial spectrum, but is partial to antibacterial.Triclosan is better than gram negative bacilli to the effect of gram positive bacteria (comprising M RSA), particularly Pseudomonas aeruginosa.Triclosan has certain activity to Mycobacterium and Candida, but to the activity of filament fungus a little less than.Studies show that in a large number, after washing one's hands with triclosan, the reduction number of bacterium colony is lower than beneficial rhzomorph, povidone iodine and alcohol product.
U.S. FDA in 2011 and the EPA safety of triclosan of reappraising, reacts with chloride tap water once come from the product that contains triclosan, can form the material that one is called as " chloroform ", i.e. chloroform.Chloroform animal experiment is found, can cause damage to heart and liver, has slight teratogenecity, can induce white mice generation hepatocarcinoma, but there is no human carcinogen's research data.For the sake of assurance, IARC and the U.S. all classify chloroform as the suspect carcinogen that human body is had to potential threat.In clinical use, due to abuse of antibiotics, variation antibacterial to be carried out to natural selection, thereby produced " superbacteria ", it can make malignant bacteria incomparably powerful, resists all antibiotic.
Therefore; exploitation safety, wide spectrum, efficient, working concentration is low, environmental friendliness, user of service's skin is had to the disinfectant of protection is the important of following clinical demand always; along with testing the increase of microorganism and disinfectant unsafe factor; requirement for experiment condition is increasingly strict, causes that new disinfectant development difficulty is large, cost is many.Also be no lack of on the market the Chinese medicine extract of containing gel, adopt several kinds of Chinese medicinal materials composite more, effect complicated, mechanism is indefinite.The invention provides a kind of definite ingredients, there is the plant dry-cleaning disinfecting gel of excellent antibacterial effect.
Chitosan (chitosan) is that the chitin (chitin) extensively being existed by nature obtains through deacetylation, chemical name is Chitosan (1-4)-2-amino-B-D glucose, from 1859, first Frenchman Rouget obtains after chitosan, biological functionality and the compatibility of this natural polymer, blood compatibility, safety, the premium properties such as microbic resolvability are by all trades and professions extensive concern, at medicine, food, chemical industry, cosmetics, water treatment, METAL EXTRACTION and recovery, the applied research of the numerous areas such as biochemistry and biomedical engineering has been obtained major progress.The bacteriostatic activity chitosan of chitosan in weak acid solvent, be easy to dissolve, it is worthy of note especially dissolve after solution in contain amino (NH2+), these amino are by carrying out anti-bacteria in conjunction with negatron.The Antibacterial activity of chitosan, makes it have a wide range of applications in fields such as medicine, weaving and food.Chitosan and derivant thereof have good antibacterial activity, can suppress the growth and breeding of some funguses, antibacterial and virus.Think that at present its possible mechanism has three: one, due to the polycation of chitosan, be easy to the group effect of fungal cell's surface band negative charge, thereby change mobility and the permeability of pathogen cell membrane; The 2nd, disturb copying and transcribing of DNA; The 3rd, the metabolism of blocking-up pathogen.In recent years, have many researcheres to propose chitosans by induction pathogenesis-related proteins, the modes such as accumulation secondary metabolite and signal conduction reach the viewpoint of antibacterial object.
Chinese patent CN1309304C discloses a kind of disinfecting gel and its preparation method and application, and it comprises staphylococcus lysozyme 0.0001-0.01wt%, lysozyme 0.01-1wt%, thickening agent 1-20wt%, emollient 0.1-15wt%, water surplus.It can be for the cleaning and disinfection of hand skin, but this disinfecting gel in use long-term killing effect is not good.
Chinese patent application CN1498538A discloses a kind of marine organism sterilizing agent taking chitin, alginic acid element, agaroidin gel sugar, DP300 DP300, cedar wood alcohol as effective ingredient, this disinfectant is non-stimulated, abnormal smells from the patient delicate fragrance, but it exists defect of virus killing poor effect for a long time equally, be difficult to adapt to the needs of prolonged operations.
Therefore, develop a kind of skin irritation little, the Novel free decontamination venom of sterilization spectrum width and long action time, is the work shouldering heavy responsibilities to vast medical matters researcher.
Summary of the invention
The invention provides the biological disposable disinfectant solution of a kind of novel sea, the biological disposable disinfectant solution of novel sea of the present invention can be used for hands sterilization or the skin degerming in disinfecting or surgery preoperative disinfection, its bactericidal effect excellence, effect is lasting, has good application prospect and economic benefit.
The present invention adopts following technical scheme to realize:
The biological disposable disinfectant solution of a kind of novel sea of the present invention, its active component is made up of chitosan, oligochitosan, baicalin, beneficial rhzomorph, polyhexamethylene guanidine, lysozyme and Rhizoma Polygoni Cuspidati extract.
Wherein:
Chitosan is by β-(1,4) the glucosamine macromole natural polymer that glycosidic bond connects, it is the unique polysaccharide with positive charge effect of occurring in nature, chitosan is by the entrained negative charge interaction of self entrained positive charge and microbial cell, destroy bacterial cell wall construction, cause cell component to leak and play antibacterial action; To there is no the microorganism of cell membrane; there is similar flocculation by penetrating in cell Yu with the biomacromolecule of anion; upset the normal physiological function of cell; blocking dna biosynthesis; thereby the Growth and Reproduction of anti-bacteria does not produce drug resistance, significantly improve Effects on local immunological functions, improve mucosa hyperemia, protect mucosa, promote epithelial cell growth.
Oligochitosan is also Chitosan oligosaccharide, also claim chitin oligosaccharide, formal name used at school β-Isosorbide-5-Nitrae-oligosaccharide-glucamine, being a kind of brand-new product that chitosan is obtained through special biological enzyme technology processing, is the low molecular weight product that water solublity is better, function is large, biological activity is high.It has the unexistent higher solubility of chitosan and easily by the function of many uniquenesses such as organism absorption, and it act as 14 times of chitosan.Prevention or treatment are by bacterial Animal diseases such as staphylococcus aureus, Escherichia coli, Actinobacillus, streptococcus mutanses.Disease resistance that can inducing plant, produces immunity and killing action to multiple fungus, antibacterial and virus.Antioxidation: can eliminate human body oxygen-derived free radicals, activation equipment somatic cell, slow down aging, suppresses skin surface harmful bacteria and grows, performance of keeping humidity excellence.
Baicalin (Baicalin) is from scutellariae,radix, to extract a kind of flavone compound of separating, has significant biological activity, has antibacterial, diuresis, antiinflammatory, resistance state and spasmolysis, and has the physiological potencies such as stronger antitumor response.Occupy critical role at clinical medicine.Baicalin can also absorb ultraviolet, removes oxygen-derived free radicals, and generation that again can check melanin, therefore both can be used for medicine, also can be used for cosmetics, is a kind of good functional cosmetics raw material.For one of Radix Scutellariae antimicrobial component, containing the clinical trachoma that is used for the treatment of of collyrium of 3% baicalin, curative effect is similar to the good medicament benemicin for the treatment of trachoma.
Benefit rhzomorph is taking Caulis Sacchari sinensis as primary raw material, is transformed into oligofructose, then is equipped with Semen Cassiae elite through the catalysis of a series of enzymes.Benefit rhzomorph is the nutrient source of bacillus bifidus, with the breeding doubly of bacillus bifidus 10-100 in rear intestinal.Bacillus bifidus is the most bellicose beneficial bacteria of inside of human body, and it the means such as engulfs by squeezing, by the harmful bacteria excretion of inside of human body or eliminate.Oligofructose is called as " beneficial bacteria factor " because of the effectiveness that bacillus bifidus is bred rapidly.Benefit rhzomorph is more outstanding one for low fructose series of products.It not only can suppress harmful bacteria growth in intestinal, promotes the rapid breeding of probiotics, improves intestine microenvironment, and can set up intestinal biological barrier, stops pathogenic bacterium invasion, prevents intractable diarrhea and constipation etc. that function of intestinal canal disorder causes.
Polyhexamethylene guanidine, has sterilization wide spectrum; Valid density is low; Speed of action is fast; Stable in properties; Premium properties soluble in water; Can use at normal temperatures; For a long time antibacterial, have no side effect; Non-corrosiveness; Colourless, odorless; Nontoxic; Do not fire, not quick-fried, use safety; Moderate; Convenient transportation, can be described as best antibacterial.In fact, this product is a kind of environment-friendly multipurpose new polymers, in industrial, agriculture, medical and daily life, has purposes extremely widely.It is polyguanidine family macromolecule polymer, in aqueous solution, can produce ionization, and its hydrophilic group part contains strong electropositive, absorption is electronegative each bacterioid, virus conventionally, enters cell membrane, suppresses film lactone plastid synthetic, cause thalline apoptosis, reach best bactericidal effect.
Lysozyme (lysozyme) claims again muramidase (muramidase) or N-acetylmuramide lycanohydrlase (N-acetylmuramide glycanohydrlase), is a kind of alkaline enzyme that can be hydrolyzed mucopolysaccharide in pathogenic bacterium.Mainly, by the-acetylmuramic acid in destruction cell wall and the β-Isosorbide-5-Nitrae glycosidic bond between NAG, make the insoluble mucopolysaccharide of cell wall resolve into solubility glycopeptide, cause cell wall rupture content to be overflowed and make bacterolysis.Lysozyme also can the direct combination with electronegative virus protein, forms double salt with DNA, RNA, apoprotein, makes virally inactivated.Therefore, this enzyme has the effects such as antibacterial, antiinflammatory, antiviral.Medically can be used as a kind of natural infection material with bactericidal action, there is antibacterial, antiviral, hemostasis, reducing swelling and alleviating pain and accelerate the effects such as organized renewing function.Clinical in chronic rhinitis, acute and chronic pharyngitis, oral ulcer, chickenpox, herpes zoster and verruca plana etc.Also can share the various antibacterials for the treatment of and viral infection with antibacterials.Oral all effective with intramuscular injection.
Emodin in Rhizoma Polygoni Cuspidati, 7-acetyl group-2-methoxyl group-6-methyl-8-hydroxyl-1,4-naphthoquinone, resveratrol all have antibacterial activity.Resveratrol In vitro Bactericidal Experiments shows: it has powerful anti-microbial property to the trichophyton rubrum, the trichophyton interdigitalis that cause stubborn dermatitis, pompholyx shape tinea alba, and to bacillus subtilis, Resina garciniae eight repeatedly bacterium etc. have stronger bactericidal action.Emodin, PD, emodin-8-glycoside have inhibitory action to staphylococcus aureus, hepatitis diplococcus.A kind of Flavonoid substances containing in Rhizoma Polygoni Cuspidati has inhibitory action to staphylococcus aureus, Staphylococcus albus, Bacillus proteus.10% Rhizoma Polygoni Cuspidati decocting liquid can II type virus (ECHO11) all have inhibitory action to herpes simplex virus, A type capital, the influenza Asia 68-1 of section virus and dust.3% decocting liquid to No. 479 adenovirus type IIIs, No. 72 poliomyelitis II types, No. 44 dusts can 9 types, 7 kinds of representative Strain such as COxsackie A9 type and B5 type, encephalitis B (Jing Wei grinds I strain), No. 140 herpes simplexes all have stronger inhibitory action.Rhizoma Polygoni Cuspidati monomer I and II can make hepatitis B antigen titre reduce by 8 times.For clearer expression Rhizoma Polygoni Cuspidati extract of the present invention, applicant provides a kind of preparation method of preparing above-mentioned Rhizoma Polygoni Cuspidati extract, its step comprises: get dry Rhizoma Polygoni Cuspidati, pulverize, sampling 10g, is contained in beaker, adds and extracts solvent 120mL, the pH value of lixiviating solution is adjusted to designated value with sodium hydroxide solution or hydrochloric acid solution, soaks 16～24h.Solution colour becomes yellowish-brown or 4 layers of filtered through gauze of brown rear use, gets 55mL filtrate and be distilled to 5mL by rotary evaporator at 50 DEG C.Cold preservation, for subsequent use.
The present invention makes disinfectant solution by above-mentioned active component composition according to optimal proportion, through test of many times and clinical verification, can either broad-spectrum high efficacy kill common pathogenic flora, reduce clinical infection probability has good histocompatibility simultaneously, and skin irritation is little.Multi-medicament component is share, and has significant synergism killing aspect common pathogen, stops sticking, breed of pathogenic bacterium, has also avoided using the drawback such as drug resistance, superinfection, anaphylaxis and the toxic and side effects to body of antibiotic initiation.
Inventor explores constituent content in above-mentioned disinfectant solution as evaluation criterion the killing effect of pathogenic bacterium taking above-mentioned disinfectant solution, inventor finds when component weight percentage in above-mentioned disinfectant solution is: chitosan 0.5%-2%, oligochitosan 1-4%, baicalin 2%-8%, benefit rhzomorph 0.5%～2%, polyhexamethylene guanidine are 0.5%～1.5%, lysozyme 2%-5%, Rhizoma Polygoni Cuspidati extract is 1.5%-5%, surplus is water, and described disinfectant solution is better to the killing effect of pathogenic bacterium.Wherein, when disinfectant solution contains chitosan 1.5%, oligochitosan 3%, baicalin 5%, beneficial rhzomorph 1.4%, polyhexamethylene guanidine are 1%, lysozyme 3%, and when Rhizoma Polygoni Cuspidati extract is 3.5%, its killing effect to pathogenic bacterium is best.
Disinfectant solution of the present invention, except containing active component, can also contain wetting agent and pH regulator.In prior art, all can be used for disinfectant solution of the present invention about wetting agent and pH regulator.Wherein said wetting agent is preferably glycerol.Described pH regulator is preferably triethanolamine, triisopropanolamine, diisopropanolamine (DIPA) or its combination.The pH of described disinfectant solution is preferably 5.0-6.5.
The biological disposable disinfectant solution of novel sea of the present invention can be widely used in skin degerming field, and its mechanism of action is:
A) chitosan is macromolecule polyalcohol, and strand disperse is stretched, and is great swelling state, and has viscosity; Polyhexamethylene guanidine has stronger biocidal property and film property.Disinfectant solution of the present invention can form the gelatinous mass of the thickness of clarification, and gel is attached to skin surface and forms protecting film, can make sterilization face be isolated from the outside, and avoids secondary cross infection.
B) this gel is subacidity, be not subject to microbiological effect, particularly multiple bacteriostatic active ingredients coupling in disinfectant solution of the present invention, not only can expand the anti-pathogenic bacterium spectrum of disinfectant solution, and can aspect antibacterial activity, there is significant collaborative reinforced effects, thereby suppress pathogen breeding, strengthen resistivity and the immunocompetence of diseased region to pathogenic bacteria, virus.
C) feature of the equal tool water-soluble base of chitosan, in performance subjective antisepsis activity, can slowly discharge parcel beneficial rhzomorph, baicalin and Rhizoma Polygoni Cuspidati extract wherein, extends the virus killing effect of compositions.
Based on this, the invention provides a kind of purposes of above-mentioned disinfectant solution, above-mentioned disinfectant solution is applied in hands sterilization or skin degerming, and wherein said hands sterilization or skin degerming can be disinfecting or surgery preoperative disinfection.The embodiment of the present invention 7 shows that disinfectant solution of the present invention all has stronger killing action to most pathogenic bacterium certificate.The embodiment of the present invention 8 shows that disinfecting gel of the present invention can effectively kill staphylococcus aureus (Staphyloccocus aureus), pseudomonas aeruginosa (Pseudomonas aeruginosa), escherichia coli (Escherichia coli) and Candida albicans (Candida albicans) etc., and sterilization 15min after and 200min after suppression ratio all reach more than 98.5%, be better than reference embodiment 1 and reference embodiment 2.Disinfecting time, the biological disposable disinfectant solution of novel sea of the present invention is more lasting action time, the bacterium colony degree of killing of 15min and 200min is more or less the same, and the bacterium colony killing rate of reference embodiment 1 and reference embodiment 2 is along with the fast reducing of prolongation of disinfecting time.The long operation preferably of the biological disposable disinfectant solution of novel sea of the present invention is used.The embodiment of the present invention 9 experimental results demonstrations, repeatedly medication is very little to skin irritation for the biological disposable disinfectant solution of novel sea of the present invention, with matched group there was no significant difference.
The using method of the biological disposable disinfectant solution of novel sea of the present invention is: 1) surgery preoperative disinfection: after washing one's hands by specification soap lye, flowing water washes down, dry with sterile towel, then get this product stock solution and be evenly applied to hands and forethiga one time, the about 5-10ml of every hands arm, acts on 3 minutes both hands rubbings to dry.2) disinfecting of hands: get this product 3ml and be applied to 1 minute rubbing of hand effect to dry.3) skin health sterilization: get this product and be applied in right amount or be sprayed on skin surface to moistening, act on 3 minutes natural dryings.
When the biological disposable disinfectant solution of novel sea of the present invention is used for hands sterilization or skin degerming, compared with prior art there is following technical advantage:
1) the biological disposable disinfectant solution of novel sea that the present invention makes by rational formula is by the multiple bacteriostatic active ingredients of coupling, in significantly expanding antimicrobial spectrum, each component obtains remarkable synergism aspect antibacterial, thereby suppress pathogen breeding, strengthen resistivity and the immunocompetence of diseased region to pathogenic bacteria, virus.
2) in the biological disposable disinfectant solution of novel sea of the present invention, active component mainly comes from nature, good biocompatibility, and side effect is low, has avoided using the drawbacks such as drug resistance, superinfection, anaphylaxis and the toxic and side effects to body that antibiotic causes.
)when the biological disposable disinfectant solution of novel sea of the present invention is used for skin degerming, its medicine irritation is little, and bactericidal activity is lasting, can significantly improve the therapeutic effect of medical and nursing work person's compliance and medicine, greatly reduces postoperative infection.
Detailed description of the invention
Further describe the present invention by detailed description of the invention below, but the present invention is not limited only to following examples.Within the scope of the invention or not departing from content of the present invention, spirit and scope, sanitizing composition of the present invention is suitably improved, replaces the component that effect is identical, within will become apparent to those skilled in the art that they are all deemed to be included in scope of the present invention.
embodiment 1: the biological disposable disinfectant solution of novel sea of the present invention
A kind of for the biological disposable disinfectant solution of novel sea, active component comprises chitosan, oligochitosan, beneficial rhzomorph, baicalin, polyhexamethylene guanidine, lysozyme, Rhizoma Polygoni Cuspidati extract.Wherein chitosan concentration is 1%, oligochitosan concentration is 3%, beneficial rhzomorph concentration is 1.2%, Determination of baicalin is 3.7%, polyhexamethylene guanidine concentration is 1%, lysozyme concentration is 2.5%, Rhizoma Polygoni Cuspidati extract 3%.
To prepare one kilogram of gel as example, in every kilogram of gel, contain 10g chitosan, 30g oligochitosan, 12g benefit rhzomorph, 37g baicalin, 10g polyhexamethylene guanidine, 25g lysozyme, 30g Rhizoma Polygoni Cuspidati extract.Concrete preparation method is that 10g chitosan, 25g lysozyme and 30g oligochitosan are added in 846 milliliters of purified water, it is fully dissolved with high speed dispersor; 12g benefit rhzomorph is well-dispersed in 50 milliliters of propylene glycol, scattered beneficial rhzomorph is added in the solution of above-mentioned abundant dissolving simultaneously, continue stirring and make mix homogeneously, the pH value of solution is between 5.0 to 6.0, and product becomes gel; Finally use the aqueous solution of 0.22um microporous filter membrane aseptic filtration polyhexamethylene guanidine and the aqueous solution of Rhizoma Polygoni Cuspidati extract, then add in gel according to the content in every kilogram of gel, finally add wetting agent and pH adjusting agent to can be made into finished product.
the biological disposable disinfectant solution of embodiment 2-embodiment 6 novel sea of the present invention
Skin antiseptic prescription is as shown in table 1, and preparation method is with the disposable disinfectant solution preparation method of embodiment 1 marine organisms.
The percentage ratio composition of table 1 embodiment 2-embodiment 6 disinfectant liquid for skin
the In Vitro Bacteriostasis experiment of embodiment 7 sanitizing compositions of the present invention
The strain that this experiment is used and culture medium are respectively α-hemolytic streptococcus (32209), beta hemolytic streptococcus (32210), colon bacillus (44102), proteus vulgaris (49101), pseudomonas aeruginosa (Pseudomonas aeruginosa), Candida albicans (Candida albicans) Salmonella paratyphi A (50001), shigella dysenteriae (51252), staphylococcus aureus [26111 (5)], hemophilus influenza (58529-4), nutrient agar (in tired pharmaceutical biological product calibrating institute), opacity tube (Chinese bacterial turbidity standard, the biological combinations calibrating of Chinese medicine institute).
In Vitro Bacteriostasis experiment: 1. prepare pastille agar plate.Tested material is pressed to the culture medium of doubling dilution preparation different pharmaceutical concentration, heating, modulation required pH value separately, 121 DEG C of sterilizing 30 min, are chilled to 45 DEG C.Pour into into aseptic flat board (plate d=65 mm, 10 ml/ plates), finally make the extension rate containing tested material stock solution (2g/ml) in culture medium be respectively l:2,1:4 ... 1:1024.Do blank culture medium separately simultaneously.2. inoculation.Get the bacterium in young age that reaches the third generation, be respectively the bacterium liquid to be checked of 108 cfu/ml by standard opacity tube corrected concentrations with 0.9% sodium chloride solution, be inoculated in respectively the agar surface of pastille separately of different doubling dilutions, inoculate parallel point 3 points simultaneously, make at every containing 2 bacterium liquid.The liquid-drop diameter of each vaccination is 5-8 mm.After vaccination is dry, upset is dull and stereotyped cultivates 20 h observed results (simultaneously doing blank) in 36 DEG C of constant incubators.3. criterion.Not occur that minimum dilution tested material concentration on the agar plate of bacterium colony is as its minimum inhibitory concentration (MIC).
In this experiment, the concentration of disinfectant solution is the total concentration of chitosan in disinfectant solution, oligochitosan, baicalin, beneficial rhzomorph, polyhexamethylene guanidine, lysozyme and Rhizoma Polygoni Cuspidati extract isoreactivity composition.Its minimal inhibitory concentration to each pathogenic strain is as shown in table 2:
The minimal inhibitory concentration of table 2 sanitizing composition of the present invention to pathogenic strain
Result demonstration, disinfectant solution of the present invention all has stronger killing action to most pathogenic bacterium certificate.
Reference embodiment 1 adopts the disinfecting gel of CN1309304C embodiment 1
Preparation method is: the content 0.01g(0.01 wt% that first takes staphylococcus lysozyme), the content 0.3g(0.3 wt% of lysozyme), the content 3g(3wt% of hydroxy propyl cellulose), glycerol 5g(5wt%), surplus is water, and mix homogeneously can obtain required disinfecting gel.
Reference embodiment 2 adopts in Chinese patent application CN1498538A and fills a prescription three
Its prescription consists of: chitin 3.0-6.0, and alginic acid element 2.0-5.0, agaroidin gel sugar 1.0-2.0, DP300 DP300 0.5-0.8, cedar wood alcohol 1.8-2.0, purified water adds to 100.
the Disinfection Effect comparison of the biological disposable disinfectant solution of embodiment 8 novel sea of the present invention
Use bacterium liquid to be measured to be evenly coated onto medical apparatus surface, and get one (0.05ml) bacterium liquid to be measured and put into culture medium flat plate and cultivate, coat rapidly the present invention's disinfectant solution of sterilizing on apparatus surface.Respectively at after 15min and be placed in flat board from apparatus surface scraping 1 (0.05ml) after 200min and cultivate.Each bacterium liquid is cultivated respectively 48 hours countings respectively under each bacterium preference temperature at flat board.Bacterium colony killing rate after calculating painting disinfectant solution after 15min and 200min.
The Disinfection Effect comparison of table 3 sanitizing composition of the present invention
Disinfecting gel free from admixture of the present invention, without particulate matter.As can be seen from Table 3, it can effectively kill staphylococcus aureus (Staphyloccocus aureus), pseudomonas aeruginosa (Pseudomonas aeruginosa), escherichia coli (Escherichia coli) and Candida albicans (Candida albicans) etc., and sterilization 15min after and 200min after suppression ratio all reach more than 98.5%, be better than reference embodiment 1 and reference embodiment 2.Disinfecting time, the present invention's disinfectant solution of sterilizing is more lasting action time, and the bacterium colony degree of killing of 15min and 200min is more or less the same, and the bacterium colony killing rate of reference embodiment 1 and reference embodiment 2 is along with the fast reducing of prolongation of disinfecting time.The present invention's disinfectant solution long operation preferably of sterilizing is used.
the repeatedly irritation test of embodiment 9 sanitizing compositions of the present invention
Laboratory animal selects regular grade white New Zealand to plant 4 of rabbit, before test, 24h shaves off back part of animal spinal column diamond wool, shave a mao scope left and right Ge Yue 3cm × 3cm, the biological disposable disinfectant solution of novel sea of the present invention when test, tested material 0.5ml(being prepared by the embodiment of the present invention 3) be evenly applied on the 2.5cm × 2.5cm skin of left side, right side compares with distilled water.Smear every day once, continuously 14d.Since second day, smear front unhairing every day, clean with warm water, after 1h, observe dermoreaction and carry out skin irritation reaction scoring, after off-test, calculate respectively 14 days total marks, 14 days every animal integral mean values, every day, every animal integral mean value, and carried out skin irritation strength grading.Repeatedly irritation test result is as shown in table 4 for the biological disposable disinfectant solution of novel sea of the present invention.
Repeatedly irritation test result of table 4 sanitizing composition of the present invention
Shown by table 4 experimental result, repeatedly medication is very little to skin irritation for the biological disposable disinfectant solution of novel sea of the present invention, with matched group there was no significant difference.
Claims (9)
1. the biological disposable disinfectant solution of novel sea, it is characterized in that: its active component is made up of chitosan, oligochitosan, baicalin, beneficial rhzomorph, polyhexamethylene guanidine, lysozyme and Rhizoma Polygoni Cuspidati extract, the each component weight percentage of described disinfectant solution active component is: chitosan 0.5%-2%, oligochitosan 1-4%, baicalin 2%-8%, benefit rhzomorph 0.5%～2%, polyhexamethylene guanidine are 0.5%～1.5%, lysozyme 2%-5%, Rhizoma Polygoni Cuspidati extract is 1.5%-5%.
2. disinfectant solution as claimed in claim 1, it is characterized in that the each component weight percentage of described disinfectant solution active component is: chitosan 1.5%, oligochitosan 3%, baicalin 5%, benefit rhzomorph 1.4%, polyhexamethylene guanidine are 1%, lysozyme 3%, and Rhizoma Polygoni Cuspidati extract is 3.5%.
3. the biological disposable disinfectant solution of novel sea as claimed in claim 1 or 2, is characterized in that: in described disinfectant solution, Rhizoma Polygoni Cuspidati extract is resveratrol.
4. the biological disposable disinfectant solution of novel sea as claimed in claim 1 or 2, is characterized in that: described disinfectant solution contains wetting agent and pH regulator.
5. the biological disposable disinfectant solution of novel sea as claimed in claim 4, is characterized in that: in described disinfectant solution, wetting agent is glycerol, and described pH regulator is triethanolamine, triisopropanolamine, diisopropanolamine (DIPA) or its combination.
6. the biological disposable disinfectant solution of novel sea as claimed in claim 1 or 2, is characterized in that: the pH of described disinfectant solution is 5.0-6.5.
7. the application of the biological disposable disinfectant solution of the novel sea described in claim 1 or 2 in skin degerming.
8. the application of the biological disposable disinfectant solution of the novel sea described in claim 1 or 2 in hands sterilization.
9. application as claimed in claim 8, is characterized in that: described hands sterilization is disinfecting or surgery preoperative disinfection.
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| CN105267273A (en) * | 2015-12-04 | 2016-01-27 | 李清源 | Natural skin-protecting wound disinfectant and preparation method thereof |
| CN105326875B (en) * | 2015-12-04 | 2019-04-16 | 山东名德医疗科技有限公司 | A kind of natural nonreactive moisturizing disinfectant liquid for skin and preparation method thereof |
| CN106074211A (en) * | 2016-07-13 | 2016-11-09 | 深圳市美福源日用品有限公司 | A kind of skin antibacterial liquid |
| CN106420923B (en) * | 2016-11-08 | 2021-04-30 | 齐国德 | Baicalein bioactive action substance and polyhexamethylene biguanide combined disinfectant and preparation method thereof |
| CN107519088A (en) * | 2017-08-30 | 2017-12-29 | 李美英 | A kind of haired pet Non-water washing cleaning-sterilizing liquid |
| CN108324933A (en) * | 2018-04-08 | 2018-07-27 | 山东鲁北药业有限公司 | Lysozyme disinfecting liquid and its preparation method and application |
| CN113209003A (en) * | 2021-04-29 | 2021-08-06 | 振德医疗用品股份有限公司 | Biological type hand-washing-free antibacterial gel, preparation method and application |
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