CN113209003A - Biological type hand-washing-free antibacterial gel, preparation method and application - Google Patents
Biological type hand-washing-free antibacterial gel, preparation method and application Download PDFInfo
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- CN113209003A CN113209003A CN202110476420.7A CN202110476420A CN113209003A CN 113209003 A CN113209003 A CN 113209003A CN 202110476420 A CN202110476420 A CN 202110476420A CN 113209003 A CN113209003 A CN 113209003A
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- lysozyme
- gel
- washing
- hand
- free
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 21
- 108010014251 Muramidase Proteins 0.000 claims description 55
- 102000016943 Muramidase Human genes 0.000 claims description 55
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 55
- 235000010335 lysozyme Nutrition 0.000 claims description 55
- 229960000274 lysozyme Drugs 0.000 claims description 54
- 239000004325 lysozyme Substances 0.000 claims description 54
- 239000002904 solvent Substances 0.000 claims description 28
- 239000003906 humectant Substances 0.000 claims description 23
- 238000003756 stirring Methods 0.000 claims description 20
- 239000008213 purified water Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000005303 weighing Methods 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
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- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- 229960005488 aceglutamide Drugs 0.000 claims description 3
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 229960001631 carbomer Drugs 0.000 claims description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 3
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 claims description 3
- 229940074393 chlorogenic acid Drugs 0.000 claims description 3
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 claims description 3
- 235000001368 chlorogenic acid Nutrition 0.000 claims description 3
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000000665 guar gum Substances 0.000 claims description 3
- 229960002154 guar gum Drugs 0.000 claims description 3
- 235000010417 guar gum Nutrition 0.000 claims description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 3
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 3
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 3
- 229960004889 salicylic acid Drugs 0.000 claims description 3
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 3
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 3
- 235000005504 Vitis heyneana Nutrition 0.000 claims description 2
- 241000728008 Vitis heyneana Species 0.000 claims description 2
- 235000019437 butane-1,3-diol Nutrition 0.000 claims description 2
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- 230000000249 desinfective effect Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 210000004400 mucous membrane Anatomy 0.000 claims description 2
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 9
- 239000000126 substance Substances 0.000 abstract description 6
- 150000003242 quaternary ammonium salts Chemical class 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 3
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- 239000000243 solution Substances 0.000 description 19
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 8
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- 241000588724 Escherichia coli Species 0.000 description 6
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- 238000012360 testing method Methods 0.000 description 3
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- 102000006410 Apoproteins Human genes 0.000 description 1
- 108010083590 Apoproteins Proteins 0.000 description 1
- XNCOSPRUTUOJCJ-UHFFFAOYSA-N Biguanide Chemical compound NC(N)=NC(N)=N XNCOSPRUTUOJCJ-UHFFFAOYSA-N 0.000 description 1
- 229940123208 Biguanide Drugs 0.000 description 1
- 229930185605 Bisphenol Natural products 0.000 description 1
- 108010065152 Coagulase Proteins 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000002068 Glycopeptides Human genes 0.000 description 1
- 108010015899 Glycopeptides Proteins 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- MNLRQHMNZILYPY-MDMHTWEWSA-N N-acetyl-alpha-D-muramic acid Chemical compound OC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@@H]1NC(C)=O MNLRQHMNZILYPY-MDMHTWEWSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 241000588770 Proteus mirabilis Species 0.000 description 1
- 241000588778 Providencia stuartii Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 240000002503 Vitis amurensis Species 0.000 description 1
- 235000004283 Vitis amurensis Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960002152 chlorhexidine acetate Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Cosmetics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a biological type hand-washing-free antibacterial gel, a preparation method and application thereof. The biological type hand-washing-free antibacterial gel does not contain any chemical disinfection active ingredients such as alcohol, quaternary ammonium salt, phenols and the like, is safer and milder, has no stimulation and stable performance, and is suitable for different crowds; meanwhile, the skin of the hand can be better protected while bacteriostasis is realized by adding the moisture-preserving component; the biological hand-washing-free bacteriostatic gel does not contain chemical components such as alcohol and the like, and can be carried on any vehicle.
Description
Technical Field
The invention belongs to the technical field of disinfectants, relates to a hand-washing-free gel, and particularly relates to a biological type hand-washing-free antibacterial gel which is safe, mild, non-irritant, stable in performance and suitable for different crowds, and a preparation method and application thereof.
Background
In life, the hands are most frequently contacted with the outside, so the hands are the most easily polluted body parts and are also important channels for transmitting germs. The hand sanitizer is a daily article specially used for hand cleaning, can keep the hand clean and sanitary, and has very important significance for preventing diseases and guaranteeing the body health.
At present, a large amount of resistant bacteria are propagated due to the fact that the disinfectant is used in large amounts in various industries and academia. According to the reports of relevant documents, various traditional chemical disinfectants have the generation of resistant bacteria, such as quaternary ammonium salt disinfectants, including pseudomonas aeruginosa, alcaligenes, candida albicans and the like, and even coagulase negative staphylococcus. Among the phenolic disinfectants, it has been reported that gram-negative bacteria can be separated from hexachloro bisphenol soap solution and cream, and become a potential cause of skin infection. The biguanide disinfectants mainly have drug resistance to chlorhexidine acetate, and comprise providencia stuartii, pseudomonas aeruginosa, proteus mirabilis, klebsiella pneumoniae, streptococcus faecalis and the like, and various disinfectants have bacterial drug resistance conditions to different degrees, thereby bringing great threat to the environment and human beings. Based on the analysis of the sterilized product sales data, people prefer to use gel-type products.
At present, 89% of gel hand disinfection and bacteriostasis products contain alcohol, so that the gel hand disinfection and bacteriostasis products can destroy sebum layers after long-term use and cannot be carried on transportation tools such as airplanes and trains, and therefore people who often go on a business are very confused. The hand disinfection and bacteriostasis product mainly takes ethanol, quaternary ammonium salts, phenols, silver ions, hypochlorous acid and the like as main components, and is not suitable for long-term use by pregnant women, children and other people.
Lysozyme (also called muramidase) or N-acetylmuramidase (N-acetylmuramidase glycohydrolase) is an alkaline enzyme that hydrolyzes mucopolysaccharides in bacteria. Lysozyme dissolves bacteria by breaking the beta-1, 4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, breaking down the cell wall insoluble mucopolysaccharide into soluble glycopeptides, causing the contents of the broken cell wall to escape. Lysozyme can also be directly combined with virus protein with negative charge, and forms a complex with DNA, RNA and apoprotein to inactivate the virus.
Disclosure of Invention
The invention aims to solve the defects that most of the existing gel hand disinfection and bacteriostasis products contain alcohol, cannot be carried on transportation tools such as airplanes and trains, and is not suitable for long-term use by people such as pregnant women, children and the like, and provides a biological type hand-washing-free bacteriostasis gel which is safe, mild, non-irritant, stable in performance and suitable for different people.
The invention also aims to provide a preparation method of the biological type hand-washing-free bacteriostatic gel.
The invention also provides application of the biological type hand-washing-free bacteriostatic gel.
In order to achieve the purpose, the invention adopts the following technical scheme:
the biological type hand-washing-free bacteriostatic gel comprises the following components in percentage by weight: 5-20% of lysozyme, 1-3% of gel, 0.01-0.10% of pH regulator, 3-8% of humectant, 10-20% of solubilizer and the balance of solvent.
In the technical scheme, the main component of the antibacterial gel is lysozyme, and the antibacterial gel does not contain alcohol and any other chemical disinfection active components, is safe and environment-friendly, and simultaneously effectively inhibits escherichia coli, candida albicans, staphylococcus aureus and pseudomonas aeruginosa, and the antibacterial rate is more than 99.9%. The biological hand-washing-free antibacterial gel has stable performance, is stored in an aging box for 90 days at 37 ℃, and has an antibacterial rate of more than 99.9% on escherichia coli, candida albicans, staphylococcus aureus and pseudomonas aeruginosa; storing the gel in an aging box for 14 days at 54 ℃, wherein the gel form is not changed; after the gel is stored in a freezing chamber of a refrigerator for 14 days and taken out for dissolution, the shape of the gel is not changed.
Through test tests, when the content of the lysozyme is lower than 5%, the sterilization rate is less than 90%, and the sterilization rate is gradually improved along with the increase of the concentration. When the concentration is more than 20 percent, the solution is turbid, so the content of the lysozyme in the product is determined to be 5 to 20 percent.
The animal-derived lysozyme has good bacteriostatic effect, the plant-derived lysozyme has good safety, and the microbial source has broad-spectrum bacteriostatic effect, so that the invention selects three kinds of lysozyme for compounding to achieve the effects of spectrum bacteriostatic and safety.
As a preferred embodiment of the invention, the lysozyme is a mixture of animal lysozyme, plant lysozyme and microbial lysozyme.
As a preferred scheme of the invention, the animal lysozyme, the plant lysozyme and the microbial lysozyme comprise the following components in percentage by weight: 1-2:1:2-1.
As a preferable scheme of the invention, the gel agent comprises one or more of guar gum, sodium carboxymethyl cellulose or carbomer.
As a preferable scheme of the invention, the pH regulator comprises one or more of chlorogenic acid, salicylic acid or citric acid which are pure plant extracts.
As a preferable scheme of the invention, the humectant comprises one or more of acetyl glutamine, sodium hyaluronate, 1, 3 butanediol, a Vitis heyneana extract or trehalose.
As a preferable embodiment of the present invention, the solubilizer includes one or more of hydrogenated castor oil, fatty alcohol-polyoxyethylene ether, fatty alcohol-polyoxyethylene-polyoxypropylene ether, polyoxyethylene sorbitan fatty acid ester or polyglycerol fatty acid ester, which are natural plant extracts.
In a preferred embodiment of the present invention, the solvent is purified water.
The preparation method of the biological type hand-washing-free bacteriostatic gel comprises the following steps:
1) weighing gel, pH regulator and solubilizer according to formula ratio, adding into a container with appropriate amount of purified water, and stirring thoroughly to swell and disperse to obtain solution A;
2) weighing lysozyme according to the formula ratio, adding the lysozyme into the solution A obtained in the step 1), and fully stirring to obtain a solution B;
3) weighing the humectant according to the formula amount, adding the humectant into the solution B obtained in the step 3), fully stirring, supplementing with purified water, and uniformly stirring to obtain the biological type hand-washing-free antibacterial gel.
The biological type hand-washing-free bacteriostatic gel is applied to cleaning and disinfecting skin or mucous membranes.
Compared with the prior art, the invention has the following beneficial effects:
1) the biological type hand-washing-free antibacterial gel does not contain any chemical disinfection active ingredients such as alcohol, quaternary ammonium salt, phenols and the like, and the antibacterial rate detection is carried out according to a test method of dissolution antibacterial (bacteriostatic) performance of disinfection technical over-specification 2..1.11.3.2, and the result shows that the biological type hand-washing-free antibacterial gel has the antibacterial rate of more than 99.9 percent on staphylococcus aureus and candida albicans and the antibacterial rate of 100 percent on escherichia coli and pseudomonas aeruginosa and conforms to the requirement of GB15979 hygienic standard for disposable sanitary products;
2) the antibacterial active ingredient is lysozyme, is safer and milder, has no irritation and stable performance, and is suitable for different people; meanwhile, the skin of the hand can be better protected while bacteriostasis is realized by adding the moisture-preserving component;
3) the biological type hand-washing-free bacteriostatic gel does not contain chemical components such as alcohol and the like, and can be carried on any vehicle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention
Example 1
The embodiment provides a biological hand-washing-free antibacterial gel, which comprises lysozyme, a gel, a pH regulator, a humectant and a solubilizer, wherein the biological hand-washing-free antibacterial gel comprises the following raw materials in percentage by weight: 5% of lysozyme, 1% of gel, 0.01% of pH regulator, 3% of humectant, 10% of solubilizer and the balance of solvent.
Wherein, the lysozyme comprises animal lysozyme, plant lysozyme and microbial lysozyme according to the proportion of 1: 1: 1; the gel is guar gum; the pH regulator is chlorogenic acid; the humectant is 1.3 butanediol and trehalose in a ratio of 1: 1, the solvent is purified water; the solubilizer comprises hydrogenated castor oil and fatty alcohol-polyoxyethylene ether in a ratio of 2: 1.
the preparation method comprises the following steps:
1) weighing gel, pH regulator and solubilizer according to formula ratio, adding into a container with appropriate amount of purified water, and stirring thoroughly to swell and disperse to obtain solution A;
2) weighing lysozyme according to the formula ratio, adding the lysozyme into the solution A obtained in the step 1), and fully stirring to obtain a solution B;
3) weighing the humectant according to the formula amount, adding the humectant into the solution B obtained in the step 3), fully stirring, supplementing with purified water, and uniformly stirring to obtain the biological type hand-washing-free antibacterial gel.
Example 2
The embodiment provides a biological hand-washing-free antibacterial gel, which comprises lysozyme, a gel, a pH regulator, a humectant and a solubilizer, wherein the biological hand-washing-free antibacterial gel comprises the following raw materials in percentage by weight: 10% of lysozyme, 2% of a gelling agent, 0.015% of a pH regulator, 5% of a humectant, 15% of a solubilizer and the balance of a solvent.
Wherein, the lysozyme comprises animal lysozyme, plant lysozyme and microbial lysozyme according to the proportion of 1: 1: 2; the gel is sodium carboxymethylcellulose; the pH regulator is salicylic acid; the humectant is a cosmetic grade vitis amurensis extract and sodium hyaluronate with the proportion of 3: 1, the solvent is purified water; the solubilizer comprises polyoxyethylene sorbitan fatty acid ester and polyglycerol fatty acid ester in a ratio of 1: 1.
the preparation method comprises the following steps:
1) weighing gel, pH regulator and solubilizer according to formula ratio, adding into a container with appropriate amount of purified water, and stirring thoroughly to swell and disperse to obtain solution A;
2) weighing lysozyme according to the formula ratio, adding the lysozyme into the solution A obtained in the step 1), and fully stirring to obtain a solution B;
3) weighing the humectant according to the formula amount, adding the humectant into the solution B obtained in the step 3), fully stirring, supplementing with purified water, and uniformly stirring to obtain the biological type hand-washing-free antibacterial gel.
Example 3
The embodiment provides a biological hand-washing-free antibacterial gel, which comprises lysozyme, a gel, a pH regulator, a humectant and a solubilizer, wherein the biological hand-washing-free antibacterial gel comprises the following raw materials in percentage by weight: 18% of lysozyme, 1.5% of gel, 0.012% of pH regulator, 7% of humectant, 18% of solubilizer and the balance of solvent.
Wherein, the lysozyme comprises animal lysozyme, plant lysozyme and microbial lysozyme in a proportion of 2:1: 2; the gel is carbomer; the pH regulator is citric acid; the humectant is acetyl glutamine and sodium hyaluronate (molecular weight 2000KDa) with the proportion of 3: 1, the solvent is purified water; the solubilizer is hydrogenated castor oil and polyglycerol fatty acid ester, and the proportion is 2: 1. the preparation method comprises the following steps:
1) weighing gel, pH regulator and solubilizer according to formula ratio, adding into a container with appropriate amount of purified water, and stirring thoroughly to swell and disperse to obtain solution A;
2) weighing lysozyme according to the formula ratio, adding the lysozyme into the solution A obtained in the step 1), and fully stirring to obtain a solution B;
3) weighing the humectant according to the formula amount, adding the humectant into the solution B obtained in the step 3), fully stirring, supplementing with purified water, and uniformly stirring to obtain the biological type hand-washing-free antibacterial gel.
Compared with the examples 1-3, the biological hand-washing-free bacteriostatic gel prepared by the invention has better effect in example 3, better bacteriostatic effect than other two groups and more stable performance. The details are shown in tables 1 and 2.
TABLE 1. examples 1-3 inhibition of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans
TABLE 2 EXAMPLES 1-3 inhibition rates of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans by aging in a 37 deg.C box for 90 days
As can be seen from the above table, the bacteriostasis rate is detected according to the testing method of dissolution antibacterial (bacteriostasis) performance of disinfection technology passing specification 2. 1.11.3.2, and the bacteriostasis rate of the biological type hand-washing-free bacteriostasis gel prepared in example 3 to staphylococcus aureus and candida albicans is more than 99.9%, the bacteriostasis rate to escherichia coli and pseudomonas aeruginosa is 100%, and the result meets the requirement of the hygienic standard of disposable sanitary products GB 15979.
While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that the foregoing and other changes, omissions and deviations in the form and detail thereof may be made without departing from the scope of this invention. Those skilled in the art can make various changes, modifications and equivalent arrangements, which are equivalent to the embodiments of the present invention, without departing from the spirit and scope of the present invention, and which may be made by utilizing the techniques disclosed above; meanwhile, any changes, modifications and variations of the above-described embodiments, which are equivalent to those of the technical spirit of the present invention, are within the scope of the technical solution of the present invention.
Claims (10)
1. The biological type hand-washing-free bacteriostatic gel is characterized by comprising the following components in percentage by weight: 5-20% of lysozyme, 1-3% of gel, 0.01-0.10% of pH regulator, 3-8% of humectant, 10-20% of solubilizer and the balance of solvent.
2. The biotype hands-free bacteriostatic gel according to claim 1, wherein the lysozyme is a mixture of animal lysozyme, plant lysozyme and microbial lysozyme.
3. The biological type hand-washing-free bacteriostatic gel according to claim 1, which is characterized in that the animal lysozyme, the plant lysozyme and the microbial lysozyme are prepared in the following proportion: 1-2:1:2-1.
4. A biotype hand-washing free bacteriostatic gel according to claim 1, 2 or 3, wherein the gel comprises one or more of guar gum, sodium carboxymethyl cellulose or carbomer.
5. The biotype handwashing-free bacteriostatic gel according to claim 1, 2 or 3, wherein the pH regulator comprises one or more of pure plant extract chlorogenic acid, salicylic acid or citric acid.
6. The biotype hand-washing-free bacteriostatic gel according to claim 1, 2 or 3, wherein the humectant comprises one or more of acetylglutamine, sodium hyaluronate, 1, 3 butanediol, Vitis heyneana extract or trehalose.
7. The biological type no-clean hand bacteriostasis gel according to claim 1, 2 or 3, wherein the solubilizer comprises one or more of natural plant extracts of hydrogenated castor oil, fatty alcohol-polyoxyethylene ether, fatty alcohol-polyoxyethylene-polyoxypropylene ether, polyoxyethylene sorbitan fatty acid ester or polyglycerol fatty acid ester.
8. The biotype hand-washing-free bacteriostatic gel according to claim 1, 2 or 3, wherein the solvent is purified water.
9. The preparation method of the biotype no-clean hand bacteriostasis gel according to claim 1, which is characterized by comprising the following steps:
1) weighing gel, pH regulator and solubilizer according to formula ratio, adding into a container with appropriate amount of purified water, and stirring thoroughly to swell and disperse to obtain solution A;
2) weighing lysozyme according to the formula ratio, adding the lysozyme into the solution A obtained in the step 1), and fully stirring to obtain a solution B;
3) weighing the humectant according to the formula amount, adding the humectant into the solution B obtained in the step 3), fully stirring, supplementing with purified water, and uniformly stirring to obtain the biological type hand-washing-free antibacterial gel.
10. The application of the biological type hand-washing-free bacteriostatic gel is characterized in that the gel is used for cleaning and disinfecting skin or mucous membrane.
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| CN114958491A (en) * | 2022-06-22 | 2022-08-30 | 浙江工业大学 | Detergent with antibacterial effect |
| CN115052578A (en) * | 2021-10-13 | 2022-09-13 | 振德医疗用品股份有限公司 | Moisturizing and non-irritating foam type hand-washing-free antibacterial liquid and preparation method thereof |
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| CN112220695A (en) * | 2020-09-24 | 2021-01-15 | 硅羿科技（上海）有限公司 | Disinfection washing-free hand cleaning gel and preparation method thereof |
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| CN1552215A (en) * | 2003-06-06 | 2004-12-08 | 上海高科联合生物技术研发有限公司 | Sterilizing gel, preparing method and application thereof |
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