CN106420923A - Skullcapflavone bioactive agent and polyhexamethylene biguanidine combined disinfection liquid and preparation method thereof - Google Patents
Skullcapflavone bioactive agent and polyhexamethylene biguanidine combined disinfection liquid and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/74—Synthetic polymeric materials
- A61K31/785—Polymers containing nitrogen
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses skullcapflavone bioactive agent and polyhexamethylene biguanidine combined disinfection liquid. The skullcapflavone bioactive agent and polyhexamethylene biguanidine combined disinfection liquid is characterized by being prepared from the following components in parts by weight: 1.5-2.5 parts of a skullcapflavone bioactive agent, 5-15 parts of polyhexamethylene biguanidine, 10 parts of chitosan, 25-60 parts of azone, 1 part of sodium polyacrylate and 957.5-911.5 parts of sterile de-ionized water; the content of the polyhexamethylene biguanidine is 99% and the molecular weight of the chitosan is 1000; and the azone is medicine-grade azone and the sodium polyacrylate is food-grade sodium polyacrylate. The invention further provides a preparation method of the disinfection liquid. The disinfection liquid disclosed by the invention is wide in applicable range, has no irritation and has good sterilization and disease-prevention effect; and the preparation method is scientific and reasonable, simple in flow and easy to operate.
Description
Technical field
The present invention relates to disinfectant solution, it is a kind of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
And preparation method thereof.
Background technology
Since the dawn of human civilization, sanitation and hygiene disinfection in a variety of ways always, maintains human health existence.Due to being subject to
Environmental pollution and the function influence of fertilizer and pesticide, the variation of phytochemistry element structure.Lead to modern humans' body chemistry element
Defect, induces various diseases in a few peoples, and especially tumor disease is the most prominent, and in rising situation.These human bodies are given birth to
The disease that reason factor causes, be chemosterilant cannot prevention and control, and itself be also cause anthropochemistry defect cause it
One, so, combined with actual nontoxic level poly hexamethylene biguanide with biological action thing gene regulation cell Radix Scutellariae ketone, in vitro
Energy sanitation and hygiene sterilizing, in vivo can gene regulation cell diseases prevention.
The present invention, with the Radix Scutellariae ketone of biological action thing diseases prevention, the disinfectant solution made, has filled up disinfectant biological activity
The blank of actor gene regulation cell diseases prevention, and improve making full use of of China's abundant cheap Radix Scutellariae resource.
Content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, provides a kind of applied widely, nonirritant, sterilizes, prevents
The good Radix Scutellariae ketone biological action thing of sick effect and poly hexamethylene biguanide combined sterilizing liquid, and provide it scientific and reasonable, stream
Journey is simple, easy-operating preparation method.
To achieve these goals, the present invention takes technical scheme below to be achieved；
A kind of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid, is characterized in that, it include with
The component of lower weight portion；Radix Scutellariae ketone biological action thing 1.5-2.5 part；Poly hexamethylene biguanide 5-15 part；10 parts of shitosan；
Azone 25-60 part；1 part of sodium polyacrylate；Sterile deionized water 957.5-911.5 part；Described poly hexamethylene biguanide content
99%；Described chitosan molecule amount 1000；Described azone is medical grade, and described sodium polyacrylate is food stage.
A kind of described Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid, is characterized in that, it
Component including following weight portion；2 parts of Radix Scutellariae ketone biological action thing；10 parts of poly hexamethylene biguanide；10 parts of shitosan；Nitrogen
25 parts of ketone；1 part of sodium polyacrylate；952 parts of sterile deionized water. described poly hexamethylene biguanide content 99%；Chitosan molecule
Amount 1000；Described azone is medical grade, and described sodium polyacrylate is food stage.
Described Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid, is characterized in that, its preparation
Method comprises the following steps；
1) preparation of Radix Scutellariae ketone biological action thing
By the scutellariae,radix section of selected processing, pulverize 60 mesh sieves, make Baical Skullcap Root, by the Radix Scutellariae powder described in weight portion
1 part of end, stirs in the deionized water putting into 8 times of Baical Skullcap Root weight, is placed in microwave applicator, in 80 DEG C of heat treated
4min, extraction 1h, 80 mesh screen separate, and obtain first time lixiviating solution；10 times of described Baical Skullcap Root weight is added in residue
Deionized water, is placed in microwave applicator, extracts 1h in 85 DEG C, and 100 mesh screen separate, and obtain second lixiviating solution；Waste closes
And secondary lixiviating solution add hydrochloric acid to adjust pH1-2,85 DEG C of insulation 0.5h, staticly settle 12h, separate supernatant net in cleaning system
Change water circulation to use, in precipitate, add the deionized water stirring separating described supernatant weight to be suspended, plus concentration 40% hydrogen-oxygen
Change sodium adjusts pH to 7, is separated the deionized water of described supernatant weight, is added concentration 40% sodium hydroxide tune pH by addition in precipitate
To 7 weight, add equivalent concentration 95% ethanol and stir to dissolving, through 100 mesh screen, be placed in microwave applicator, plus
Heat, to 60 DEG C, adjusts pH1-2 with hydrochloric acid, is incubated 0.5h, so that precipitation is fully separated out, precipitates washed with EtOH to pH7, and ethanol is washed
Liquid separates sends cleaning system process ethanol and water circulation use back to, and paste precipitate is pulverized through 65 DEG C of evacuation dryings to constant weight,
Obtain Radix Scutellariae ketone biological action thing；
2) preparation of a liquid
Weigh 652 parts of sterile deionized water of weight, put into 10 parts of poly hexamethylene biguanides, be heated up to poly hexamethylene biguanide
Dissolving, when temperature reaches 80 DEG C, is slowly added to 2 parts of Radix Scutellariae ketone biological action thing stirrings, microwave treatment 2min, treats Radix Scutellariae ketone
After biological action thing is completely dissolved, adjust pH6-7 with citric acid, obtain a liquid；
3) preparation of b liquid
Weigh the dilute molten 10 parts of shitosans of 200 parts of deionized waters, obtain b liquid；
4) preparation of c liquid
Weigh the dilute molten 1 part of sodium polyacrylate of 100 parts of sterile deionized water of weight, obtain c liquid；
5) preparation of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
By step 2) a liquid prepared, under room temperature, add the azone of 25 parts of weight, and sequentially add step 3) the b liquid prepared
With step 4) the c liquid prepared is mixed, and obtains final product Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
Finished product.
The method have the benefit that；Because the Radix Scutellariae ketone biological action thing of the present invention is double with polyhexamethylene
In guanidine combined sterilizing liquid, the primary bioactive components baicalin of the gene regulation cell Radix Scutellariae ketone of thing containing biological action
22.02mg/L, baicalin 1486.34mg/L, by skin transport way donor internal circulation system, and through test case checking：
Cyclic metabolism in human body for the Radix Scutellariae ketone biological action thing concentration, serves blocking-up and causes inflammatory cell signal path, adjusts thin
Born of the same parents' wadding disorderly, stops the prophylaxis effect causing inflammatory cell to generate.To white staphylococcus A TCC8032, Pseudomonas aeruginosa
(ATCC15442), Candida albicans (ATCC10231), staphylococcus aureuses (ATCC6538), escherichia coli
(ATCC8099), bacillus subtilises (ATCC9372) suspension method kills bacterium logarithm value >=5；Support methods >=4, better than prior art
Similar biguanide disinfectant national standard escherichia coli (ATCC8099), staphylococcus aureuses (ATCC6538), P. aeruginosa
Bacterium (ATCC15442), Candida albicans (ATCC10231)；Support methods killing rate logarithm value >=3；Suspension method removes Candida albicans
Kill outside bacterium logarithm value >=4, other killing bacterium logarithm value all >=5, compensate for poly hexamethylene biguanide and kill hay bacterial spore
Defect；This Radix Scutellariae ketone biological active matter main component baicalin and baicalin, can be right under its effective active thing activity
Cell wadding is disorderly, virus produces, oxygen-derived free radicals form and carry out gene cell regulation and control, especially to great disease pathology phase crowd, can
Carry out the cell regulate and control under Gene Handling, to great control and prevention of disease, there is important function.Its disinfectant solution is applied widely, non-stimulated
Property, sterilization, protection effect are good；Preparation method is scientific and reasonable, and flow process is simple, easy to operate.
Brief description
Fig. 1 is that the Radix Scutellariae ketone biological action thing being recorded using high performance liquid chromatograph is combined with poly hexamethylene biguanide
The content schematic diagram of baicalin in disinfectant solution；
Fig. 2 is that the Radix Scutellariae ketone biological action thing being recorded using high performance liquid chromatograph is combined with poly hexamethylene biguanide
The content schematic diagram of baicalin in disinfectant solution；
Fig. 3 is the baicalin standard working curve schematic diagram drawn using high performance liquid chromatograph；
Fig. 4 is the baicalin standard working curve schematic diagram drawn using high performance liquid chromatograph.
Specific embodiment
Below with drawings and Examples, the invention will be further described.
A kind of Radix Scutellariae ketone biological action thing of the present invention and poly hexamethylene biguanide combined sterilizing liquid, including following weight
The component of amount part；Radix Scutellariae ketone biological action thing 1.5-2.5 part；Poly hexamethylene biguanide 5-15 part；10 parts of shitosan；Azone
25-60 part；1 part of sodium polyacrylate；Sterile deionized water 957.5-911.5 part；Described poly hexamethylene biguanide content 99%；Institute
State chitosan molecule amount 1000；Described azone is medical grade, and described sodium polyacrylate is food stage.
The Radix Scutellariae ketone biological action thing of embodiment 1 and poly hexamethylene biguanide combined sterilizing liquid, including following weight
The component of part；2 parts of Radix Scutellariae ketone biological action thing；10 parts of poly hexamethylene biguanide；10 parts of shitosan；25 parts of azone；Poly- third
1 part of olefin(e) acid sodium；952 parts of sterile deionized water. described poly hexamethylene biguanide content 99%；Chitosan molecule amount 1000；Described
Azone be medical grade, described sodium polyacrylate is food stage.
The Radix Scutellariae ketone biological action thing of the present invention is raw materials used with poly hexamethylene biguanide combined sterilizing liquid to be easy to get, all
For commercially available prod.
The Radix Scutellariae ketone biological action thing of embodiment 1 and the preparation method of poly hexamethylene biguanide combined sterilizing liquid, bag
Include following steps；
1) preparation of Radix Scutellariae ketone biological action thing
By the scutellariae,radix section of selected processing, pulverize 60 mesh sieves, make Baical Skullcap Root, by the Radix Scutellariae powder described in weight portion
2 parts of end, stirs in the deionized water putting into 8 times of Baical Skullcap Root weight, is placed in microwave applicator, in 80 DEG C of heat treated
4min, extraction 1h, 80 mesh screen separate, and obtain first time lixiviating solution；10 times of described Baical Skullcap Root weight is added in residue
Deionized water, is placed in microwave applicator, extracts 1h in 85 DEG C, and 100 mesh screen separate, and obtain second lixiviating solution；Waste closes
And secondary lixiviating solution add hydrochloric acid to adjust pH1-2,85 DEG C of insulation 0.5h, staticly settle 12h, separate supernatant net in cleaning system
Change water circulation to use, in precipitate, add the deionized water stirring separating described supernatant weight to be suspended, plus concentration 40% hydrogen-oxygen
Change sodium adjusts pH to 7, is separated the deionized water of described supernatant weight, is added concentration 40% sodium hydroxide tune pH by addition in precipitate
To 7 weight, add equivalent concentration 95% ethanol and stir to dissolving, through 100 mesh screen, be placed in microwave applicator, plus
Heat, to 60 DEG C, adjusts pH1-2 with hydrochloric acid, is incubated 0.5h, so that precipitation is fully separated out, precipitates washed with EtOH to pH7, and ethanol is washed
Liquid separates sends cleaning system process ethanol and water circulation use back to, and paste precipitate is pulverized through 65 DEG C of evacuation dryings to constant weight,
Obtain Radix Scutellariae ketone biological action thing；
2) preparation of a liquid
Weigh 652 parts of sterile deionized water of weight, put into 10 parts of poly hexamethylene biguanides, be heated up to poly hexamethylene biguanide
Dissolving, when temperature reaches 80 DEG C, is slowly added to 2 parts of Radix Scutellariae ketone biological action thing stirrings, microwave treatment 2min, treats Radix Scutellariae ketone
After biological action thing is completely dissolved, adjust pH6-7 with citric acid, obtain a liquid；
3) preparation of b liquid
Weigh the dilute molten 10 parts of shitosans of 200 parts of deionized waters, obtain b liquid；
4) preparation of c liquid
Weigh the dilute molten 1 part of sodium polyacrylate of 100 parts of sterile deionized water of weight, obtain c liquid；
5) preparation of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
By step 2) a liquid prepared, under room temperature, add the azone of 25 parts of weight, and sequentially add step 3) the b liquid prepared
With step 4) the c liquid prepared is mixed, and obtains final product Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
Finished product.
Embodiment 2 Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid, including following weight portion
Component；1.5 parts of Radix Scutellariae ketone biological action thing；5 parts of poly hexamethylene biguanide；10 parts of shitosan；1 part of sodium polyacrylate；
60 parts of penetrating agent azone；922.5 parts of sterile deionized water；Described poly hexamethylene biguanide content 99%；Described chitosan molecule
Amount 1000；Described azone is medical grade, sodium polyacrylate is food stage.
The Radix Scutellariae ketone biological action thing of embodiment 2 and the preparation method of poly hexamethylene biguanide combined sterilizing liquid, bag
Include following steps；
1) preparation of Radix Scutellariae ketone biological action thing
By the scutellariae,radix section of selected processing, pulverize 60 mesh sieves, make Baical Skullcap Root, by the Radix Scutellariae powder described in weight portion
1.5 parts of end, stirs in the deionized water putting into 8 times of Baical Skullcap Root weight, is placed in microwave applicator, at 80 DEG C of heating
Reason 4min, extraction 1h, 80 mesh screen separate, and obtain first time lixiviating solution；10 times of described Baical Skullcap Root weight is added in residue
Deionized water, be placed in microwave applicator, in 85 DEG C extract 1h, 100 mesh screen separate, obtain second lixiviating solution；Waste
Merge secondary lixiviating solution and add hydrochloric acid to adjust pH1-2,85 DEG C are incubated 0.5h, staticly settle 12h, separate supernatant in cleaning system
Purified water recycles, and adds the deionized water stirring separating described supernatant weight to be suspended in precipitate, plus concentration 40% hydrogen
Sodium oxide adjusts pH to 7, is separated the deionized water of described supernatant weight, is added concentration 40% sodium hydroxide tune by addition in precipitate
The weight of pH to 7, adds equivalent concentration 95% ethanol and stirs to dissolving, through 100 mesh screen, be placed in microwave applicator,
It is heated to 60 DEG C, adjusts pH1-2 with hydrochloric acid, be incubated 0.5h, so that precipitation is fully separated out, precipitates washed with EtOH to pH7, ethanol water
Washing liquid separates sends cleaning system process ethanol and water circulation use back to, and paste precipitate is through 65 DEG C of evacuation dryings to constant weight powder
Broken, obtain Radix Scutellariae ketone biological action thing；
2) preparation of a liquid
Weigh 622.5 parts of sterile deionized water of weight, put into 5 parts of poly hexamethylene biguanides, be heated up to polyhexamethylene double
Guanidine dissolves, and when temperature reaches 80 DEG C, is slowly added to 1.5 parts of Radix Scutellariae ketone biological action thing stirrings, microwave treatment 2min, treats Huang
After a kind of reed mentioned in ancient books ketone biological action thing is completely dissolved, adjusts pH6-7 with citric acid, obtain a liquid；
3) preparation of b liquid
Weigh the dilute molten 10 parts of shitosans of 200 parts of deionized waters, obtain b liquid；
4) preparation of c liquid
Weigh the dilute molten 1 part of sodium polyacrylate of 100 parts of sterile deionized water of weight, obtain c liquid；
5) preparation of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
By step 2) a liquid prepared, under room temperature, add the azone of 25 parts of weight, and sequentially add step 3) the b liquid prepared
With step 4) the c liquid prepared is mixed, and obtains final product Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
Finished product.
The Radix Scutellariae ketone biological action thing of embodiment 3 and poly hexamethylene biguanide combined sterilizing liquid, including following weight
The component of part；2.5 parts of Radix Scutellariae ketone biological action thing；15 parts of poly hexamethylene biguanide；Shitosan 10；40 parts of azone；Poly- third
1 part of olefin(e) acid sodium；931.5 parts of sterile deionized water, described poly hexamethylene biguanide content 99%；Described chitosan molecule amount
1000；Described azone is medical grade, sodium polyacrylate is food stage.
The Radix Scutellariae ketone biological action thing of embodiment 3 and poly hexamethylene biguanide combined sterilizing liquid and preparation method thereof, including
Following steps；
1) preparation of Radix Scutellariae ketone biological action thing
By the scutellariae,radix section of selected processing, pulverize 60 mesh sieves, make Baical Skullcap Root, by the Radix Scutellariae powder described in weight portion
2.5 parts of end, puts in 8 times of deionized waters and stirs, and is heated to 80 DEG C of microwave treatment 4min extraction 1h, and filtration separation obtains the
Lixiviating solution；The deionized water adding 10 times of described Baical Skullcap Root weight in residue extracts 1h in 85 DEG C, and filtration separation obtains the
Secondary lixiviating solution；Waste merges secondary lixiviating solution, and pH1-2,80 DEG C of insulation 0.5h are adjusted in salt adding acid, staticly settle 12h.Separate supernatant
Liquid recycles in cleaning system purified water, adds the deionized water stirring separating described supernatant weight to be suspended in precipitate,
Adjust pH to 7 with concentration 40% sodium hydroxide, adding equivalent 95% ethanol stirring and dissolving, through 100 mesh screen, be heated to 60
DEG C, adjust pH1-2 with hydrochloric acid, be incubated 0.5h, so that precipitation is fully separated out, precipitates washed with EtOH to pH to 7, ethanol water lotion divides
From sending back to, cleaning system processes ethanol and water circulation uses, and paste precipitate is pulverized through 65 DEG C of evacuation dryings to constant weight, obtains final product
Radix Scutellariae ketone biological action thing.
2) preparation of a liquid
Weigh 631.5 parts of sterile deionized water of weight, put into 15 parts of poly hexamethylene biguanides, be heated up to polyhexamethylene double
Guanidine dissolves, and when temperature reaches 90 DEG C, is slowly added to 2.5 parts of Radix Scutellariae ketone biological action thing stirrings, microwave treatment 4min, treats Huang
After a kind of reed mentioned in ancient books ketone biological action thing is completely dissolved, adjusts pH6-7 with citric acid, obtain a liquid；
3) preparation of b liquid
Weigh the dilute molten 10 parts of shitosans of 200 parts of deionized waters, obtain b liquid；
4) preparation of c liquid
Weigh the dilute molten 1 part of sodium polyacrylate of 100 parts of sterile deionized water of weight, obtain c liquid；
5) preparation of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
By step 2) a liquid prepared, under room temperature, add the azone of 40 parts of weight, and sequentially add step 3) the b liquid prepared
With step 4) the c liquid prepared is mixed, and obtains final product Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
Finished product.
Experiment case；
(1) the bleeding type wound that experimenter is caused because of pedicure toenail by itself, the toe redness radioactivity pain causing
Bitterly, instep is red and swollen, and foot dare not land, the Radix Scutellariae ketone biological action thing through the present invention and poly hexamethylene biguanide combined sterilizing
Liquid is spaced 4 hours secondary spray treatment, and after 24 hours, red and swollen disappearance wound forms a scab whole foot recovery normally；
(2) experimenter causes the Radix Scutellariae ketone biological action thing and poly- six through the present invention for the toothache through red swelling of gingiva again
After methylene biguanide combined sterilizing liquid is spaced 2 hours three times spray treatment, backteeth pain feel in 10 minutes for the first time fades away, and 24
After hour, red swelling of gingiva disappears.
Above-mentioned experiment case prove through Radix Scutellariae ketone biological action thing diseases prevention disinfectant solution to affected part spray treatment after, Radix Scutellariae
Ketone biological action thing is good with disinfectant synergism external damaged skin pathogenic bacterium bactericidal effect.Also demonstrate Huang simultaneously
The gene cell regulating and controlling effect of a kind of reed mentioned in ancient books ketone primary bioactive components actor.Achieve present invention sterilization, sterilization, the effect of diseases prevention
Really.
With reference to Fig. 1, the Radix Scutellariae ketone biological action thing of the present invention and poly- six methylenes are recorded using high performance liquid chromatograph
In base biguanide combined sterilizing liquid, the content of baicalin is 22.02mg/l.
With reference to Fig. 2, using high performance liquid chromatograph record the present invention Radix Scutellariae ketone biological action thing sub- with poly- six
In methyl biguanide combined sterilizing liquid, the content of baicalin is 29.72683 × 50=1486.34mg/l.
With reference to Fig. 3, shown using the baicalin standard working curve that high performance liquid chromatograph is drawn, baicalin：10、25、
50、100、200mg/l.
With reference to Fig. 4, shown using the baicalin standard working curve that high performance liquid chromatograph is drawn, baicalin：5、12.5、
25、50、100mg/l.
The Radix Scutellariae ketone biological action thing of the present invention and poly hexamethylene biguanide combined sterilizing liquid were in October 31 in 2016
Day, through Beihua University's medical college according to Ministry of Health of the People's Republic of China《Disinfection technology standard》(version in 2002) quantitative sterilization tries
Proved recipe method assay：It is shown in Table 1.
Table 1 killing microorganisms index
Testing result shows：The Radix Scutellariae ketone biological action thing of the present invention is excellent with poly hexamethylene biguanide combined sterilizing liquid
In prior art similar biguanide disinfectant national standard escherichia coli (ATCC8099), staphylococcus aureuses (ATCC6538),
Pseudomonas aeruginosa (ATCC15442), Candida albicans (ATCC10231)；Support methods killing rate logarithm value >=3；Suspension method removes
Candida albicans are killed outside bacterium logarithm value >=4, and other killing bacterium logarithm value all >=5, compensate for poly hexamethylene biguanide killing withered
The defect of careless bacterial spore；This Radix Scutellariae ketone biological active matter main component baicalin and baicalin are dense in the effect of its effective active thing
Under degree, can random to cell wadding, viral produce, oxygen-derived free radicals form and carry out gene cell regulation and control, especially to great disease pathology
Phase crowd, can carry out the cell regulate and control under Gene Handling, have important function to great control and prevention of disease.
Embodiments of the invention are only used for that the present invention is further illustrated, not exhaustive, and not constituting will to right
Ask the restriction of protection domain, the enlightenment that those skilled in the art obtain according to embodiments of the present invention, without creative work just
It is conceivable that the substantially equivalent replacement of other, all in the scope of the present invention.
Claims (3)
1. a kind of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid, is characterized in that, it includes following
The component of weight portion；Radix Scutellariae ketone biological action thing 1.5-2.5 part；Poly hexamethylene biguanide 5-15 part；10 parts of shitosan；Nitrogen
Ketone 25-60 part；1 part of sodium polyacrylate；Sterile deionized water 957.5-911.5 part；Described poly hexamethylene biguanide content 99%；
Described chitosan molecule amount 1000；Described azone is medical grade, and described sodium polyacrylate is food stage.
2. a kind of Radix Scutellariae ketone biological action thing according to claim 1 and poly hexamethylene biguanide combined sterilizing liquid,
It is characterized in that, it includes the component of following weight portion；2 parts of Radix Scutellariae ketone biological action thing；10 parts of poly hexamethylene biguanide；Shell
10 parts of polysaccharide；25 parts of azone；1 part of sodium polyacrylate；952 parts of sterile deionized water. described poly hexamethylene biguanide content 99%；
Chitosan molecule amount 1000；Described azone is medical grade, and described sodium polyacrylate is food stage.
3. a kind of Radix Scutellariae ketone biological action thing according to claim 2 and poly hexamethylene biguanide combined sterilizing liquid,
It is characterized in that, its preparation method comprises the following steps；
1) preparation of Radix Scutellariae ketone biological action thing
By the scutellariae,radix section of selected processing, pulverize 60 mesh sieves, make Baical Skullcap Root, by the Baical Skullcap Root 1 described in weight portion
Part, stir in the deionized water putting into 8 times of Baical Skullcap Root weight, be placed in microwave applicator, in 80 DEG C of heat treated
4min, extraction 1h, 80 mesh screen separate, and obtain first time lixiviating solution；10 times of described Baical Skullcap Root weight is added in residue
Deionized water, is placed in microwave applicator, extracts 1h in 85 DEG C, and 100 mesh screen separate, and obtain second lixiviating solution；Waste closes
And secondary lixiviating solution add hydrochloric acid to adjust pH1-2,85 DEG C of insulation 0.5h, staticly settle 12h, separate supernatant net in cleaning system
Change water circulation to use, in precipitate, add the deionized water stirring separating described supernatant weight to be suspended, plus concentration 40% hydrogen-oxygen
Change sodium adjusts pH to 7, is separated the deionized water of described supernatant weight, is added concentration 40% sodium hydroxide tune pH by addition in precipitate
To 7 weight, add equivalent concentration 95% ethanol and stir to dissolving, through 100 mesh screen, be placed in microwave applicator, plus
Heat, to 60 DEG C, adjusts pH1-2 with hydrochloric acid, is incubated 0.5h, so that precipitation is fully separated out, precipitates washed with EtOH to pH7, and ethanol is washed
Liquid separates sends cleaning system process ethanol and water circulation use back to, and paste precipitate is pulverized through 65 DEG C of evacuation dryings to constant weight,
Obtain Radix Scutellariae ketone biological action thing；
2) preparation of a liquid
Weigh 652 parts of sterile deionized water of weight, put into 10 parts of poly hexamethylene biguanides, be heated up to poly hexamethylene biguanide molten
Solution, when temperature reaches 80 DEG C, is slowly added to 2 parts of Radix Scutellariae ketone biological action thing stirrings, microwave treatment 2min, treats that Radix Scutellariae ketone is given birth to
After thing active function thing is completely dissolved, adjust pH6-7 with citric acid, obtain a liquid；
3) preparation of b liquid
Weigh the dilute molten 10 parts of shitosans of 200 parts of deionized waters, obtain b liquid；
4) preparation of c liquid
Weigh the dilute molten 1 part of sodium polyacrylate of 100 parts of sterile deionized water of weight, obtain c liquid；
5) preparation of Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid
By step 2) a liquid prepared, under room temperature, add the azone of 25 parts of weight, and sequentially add step 3) the b liquid prepared and step
C liquid prepared by rapid 4) is mixed, and obtains final product Radix Scutellariae ketone biological action thing and poly hexamethylene biguanide combined sterilizing liquid finished product.
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