CN105169464B - A kind of natural honey wound dressing and preparation method and application - Google Patents

A kind of natural honey wound dressing and preparation method and application Download PDF

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Publication number
CN105169464B
CN105169464B CN201510617897.7A CN201510617897A CN105169464B CN 105169464 B CN105169464 B CN 105169464B CN 201510617897 A CN201510617897 A CN 201510617897A CN 105169464 B CN105169464 B CN 105169464B
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honey
dressing
wound
nystatin
natural honey
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CN105169464A (en
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耿爱香
张成岗
徐向英
刘英男
李军怀
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Suzhou Sciscape Bio Pharmaceutical Technology Co ltd
Institute of Radiation Medicine of CAMMS
Tianjin Baodi Hospital
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Suzhou Sciscape Bio Pharmaceutical Technology Co ltd
Institute of Radiation Medicine of CAMMS
Tianjin Baodi Hospital
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Abstract

The invention discloses a kind of natural honey wound dressing and preparation method and application, including honey and nystatin, the concentration of nystatin is 10mg/L 50mg/L.Nystatin is added in the natural honey wound dressing of the present invention, when dressing acts on wound, nystatin can suppress the growth of fungi in honey, so as to avoid wound from having new infection.The preparation method of natural honey wound dressing of the present invention is simple and practicable at the same time, eliminates60Co gamma-rays irradiates this step of being difficult to carry out, additionally it is possible to reach through60The fungistatic effect of Co gamma-rays irradiation, remarkable in economical benefits.

Description

A kind of natural honey wound dressing and preparation method and application
Technical field
The present invention relates to biomedicine field, more particularly to a kind of natural honey wound dressing and preparation method thereof with answering With.
Background technology
Honey is a kind of complicated component, supersaturated sugar juice full of nutrition, has high osmotic pressure and relatively low pH Value, contains hydrogen peroxide (hydrogen peroxide, H2O2) and the sterilization such as acetone aldehydes (methylglyoxal, MGO) into Point, the antibacterial activity with wide spectrum, and its antibacterial activity does not produce the danger of drug resistance.
Honey is widely used in Wound healing and bone regeneration with the antibacterial activity of its wide spectrum and the advantages that being not likely to produce drug resistance, not only may be used For the treatment of topical acute wound infection, the treatment to wound surface in refractory to treatment can more embody its advantage, can pass through antibacterial, autolytic debridement Promote wound healing with processes such as promotion tissue growths.Honey sees (Efem in 1988 applied to Wound healing and bone regeneration earliest SE.Clinical observations on the wound healing properties of honey.Br J Surg, 1988,75(7):679-681.), used at that time is unprocessed honey, by being found to 59 refractory Wound healing and bone regenerations, bee Honey can quickly remove the decay and necrosis tissue instead fresh granulation, while epithelium can also be promoted to increase and absorb wound of the surface of a wound The oedema of surrounding.Afterwards, honey is increasingly favored be subject to Wound healing and bone regeneration person.Manuka honey in 2007 obtain U.S.'s food and Certification (the Pieper B.Honey-Based Dressings and Wound Care an Option of drug administration (FDA) for Care in the United States.J Wound Ostomy Cont,2009,36(1):60-66;quiz 67- 68.) honey dressing, is made to become one of selection of wound care, honey dressing is registered in Australia, Europe, North America at present.
Honey has many pathogenic bacteria pathogenic bacteria in bactericidal activity, including salmonella, Shigella and enteron aisle such as The Gram-negative such as Escherichia and comma bacillus and positive bacteria, so, although honey be easy in process of production it is each Kind of microbial contamination, however general microorganism can not in honey growth and breeding, such as fungal spore quasi-microorganism.Therefore, it is The microorganism in honey is killed, at present, medical grade honey wound dressing generally need to be through too high dose60The irradiation of Co- gamma-rays is gone out Bacterium, but high dose60The condition of Co- gamma-rays radiation sterilizations only has Large-scale professional mechanism just to possess, and the instrument cost used is high, Therefore the cost of honey wound dressing is added.If but without high dose60Co- gamma-rays irradiates, and honey wound dressing may Fresh wound can be caused to infect, honey wound dressing is lost the meaning of treat wound.
Therefore, research and develop a kind of new without high dose60Co- gamma-rays irradiates, the bee that will not also cause fresh wound to infect Sweet wound dressing has important practical significance.
The content of the invention
The purpose of the present invention is for technological deficiency existing in the prior art, there is provided a kind of not have to the use through radiation exposure Not causing the natural honey wound dressing of new wound infection, including honey and nystatin, the honey when Wound healing and bone regeneration is Without any diluted natural honey, the concentration of nystatin is 10-50mg/mL in the natural honey wound dressing.
Further include pharmaceutically acceptable auxiliary material, the auxiliary material preferred thickener, the preferred alginates of the thickener and/or Alginate dressing.
Final concentration of the alginates in the natural honey wound dressing is not higher than 0.5wt%, and the alginates are excellent Select but be not limited to sodium alginate, is one or more of in potassium alginate and calcium alginate.
The alginate dressing is the sheet cavernous body using alginates as main component, per cm2On the alginate dressing Carry the nystatin of 0.5-1mL natural honeys and concentration for 10-50mg/mL.
Including natural honey, concentration is the nystatin and alginate dressing of 20-50mg/mL, nystatin and natural bee Honey is pressed per mL natural honeys 2cm2The ratio of alginate dressing is infiltrated in the alginate dressing;
Or including natural honey, concentration is the nystatin and alginate dressing of 20-40mg/mL, nystatin and natural Honey is pressed per mL natural honeys 1.5cm2The ratio of alginate dressing is infiltrated in the alginate dressing;
Natural honey is preferably included, concentration is the nystatin and alginate dressing of 20mg/mL, nystatin and natural bee Honey is pressed per mL natural honeys 1.5cm2The ratio of alginate dressing is infiltrated in the alginate dressing.
By natural honey, concentration is that the nystatin of 20-50mg/mL and the alginates of 2wt%-5wt% mix;
Or by natural honey, concentration is that the nystatin of 20-40mg/mL and the alginates of 3wt%-5wt% mix;
It is preferred that by natural honey, concentration is that the nystatin of 20mg/mL and the alginates of 4wt% mix.
Further object of the present invention is to provide the method for preparing above-mentioned natural honey wound dressing, by nystatin and day Right honey is uniformly mixed, and the concentration of nystatin is 10-50mg/L after mixing, that is, obtains natural honey wound dressing.
Nystatin and natural honey can also be infiltrated alginate dressing after mixing, obtain natural honey wound and apply Material, the usage amount of the alginate dressing is 1-2cm2Alginate dressing/mL natural honeys.
Or, be uniformly mixed natural honey, nystatin and alginates, obtain natural honey wound dressing;Wherein, mix The concentration of nystatin is 10-50mg/L afterwards, and the concentration of alginates is not higher than 0.5wt%.
Another object of the present invention is to provide above-mentioned natural honey wound dressing prepare wound infection, chronic wounds or Application in acute wounds.
Compared with prior art, the beneficial effects of the invention are as follows:
Nystatin is added in the natural honey wound dressing of the present invention, when dressing acts on wound, nystatin It can suppress the growth of fungi in honey, so as to avoid wound from having new infection.While natural honey wound dressing of the present invention Preparation method is simple and practicable, eliminates60Co- gamma-rays irradiates this step of being difficult to carry out, additionally it is possible to reach through60Co- γ are penetrated The fungistatic effect of line irradiation, remarkable in economical benefits.
Brief description of the drawings
Fig. 1 show growing state-time diagram that Escherichia coli are cultivated in different honey density natural honeys;
Fig. 2 show the growing state that Escherichia coli are cultivated in the natural honey that honey density is 5%, 10%, 20% Oil mirror observation figure;
Fig. 3 show the life that Escherichia coli are cultivated in the natural honey that honey density is 30%, 40%, 50%, 60% Long situation;
Fig. 4 show growing state-time diagram of microorganism in different honey density natural honeys;
Fig. 5 show the growing state oil mirror observation figure of microorganism in different honey density natural honeys;
Fig. 6 show growing state-time diagram of microorganism after addition nystatin in different honey density natural honeys;
Fig. 7 show different honey density natural honey warps60The growing state of microorganism after Co- gamma-rays radiation sterilizations- Time diagram;
Fig. 8 show growing state-time diagram of microorganism after addition Buddhist health azoles in different honey density natural honeys.
Embodiment
Inventor has done following experiment in the research process for carrying out the present invention.
Experimental example one:
Comprise the following steps that:
Step 1: preparing LB fluid nutrient mediums, (10g/L containing tryptone, yeast extract 5g/L, NaCl 10g/L, are used Water is prepared, after mixing autoclaving, up to);
Step 2: e. coli bl21 (DE3) bacterium solution of 100 μ L is added to the LB Liquid Cultures of 10mL step 1 preparation (e. coli bl21 (DE3) bacterium solution in base:LB fluid nutrient medium=1:100, v/v), 37 DEG C of 200rpm shake bacterium 10-12h, obtain Escherichia coli bacteria liquid;
Step 3: the LB that natural honey (the produced natural honey of apis cerana obtained at beekeeper) is prepared with step 1 Fluid nutrient medium divides gradient dilution, obtain honey density (volumn concentration) for 80%, 70%, 60%, 50%, 40%, 30%th, a series of 20%, 10%, 5% honey samples, separately take LB fluid nutrient mediums as honey density (volumn concentration) For 0% honey sample, that is, control group;
Obtained Step 4: being separately added into step 2 in each honey density honey sample (0-80%) that 2mL step 3 prepares 20 μ L of Escherichia coli bacteria liquid (the 20 μ L arrived:2mL=1:100) a series of honey bacteria liquid samples, are obtained, microorganism is put into after mixing (model FP-1100-C, is produced growth curve detector for Helsinki, Finland (Oy Growth Curves Ab Ltd) company Product), 37 DEG C of 200rpm shake bacterium 72h, and every 4h detects once each honey bacteria liquid sample OD after the completion of shaking bacterium600Value.
It turns out that honey density be 30%-60% honey bacteria liquid sample after 40h OD600The quick rise of value, and Exceed control group, the result is shown in Figure 1 rapidly;Oil mirror is observed under (1000 ×), finds the honey bacterium solution that honey density is 5%-20% There are Escherichia coli in sample, the result is shown in Fig. 2;And find " special bacterium in the honey bacteria liquid sample that honey density is 30%-60% Kind ", doubtful saccharomycete, the result is shown in Fig. 3;Both without Escherichia coli in honey bacteria liquid sample of the honey density more than 70%, also do not send out Now it is somebody's turn to do " special strain ".
The honey sample (0-80%) for each honey density that step 3 is prepared is directly placed into microbial growth curve detection Instrument, 37 DEG C of 200rpm shake bacterium 72h, and once each honey sample OD is detected per 4h600Value.It turns out that honey density 30%- 60% honey sample OD after 40h600The still quick rise of value, the honey sample of remaining concentration is then without this phenomenon, and the result is shown in figure 4;Oil mirror is observed under (1000 ×), and honey density is to remain to find " special strain " in the honey bacteria liquid sample of 30%-60%, knot Fruit sees Fig. 5.To the honey sample Colony Culture that honey density is 30%-60%, 26s rDNA D1/D2 region sequences are expanded, and Recycling sequencing, it is maqting type saccharomycete (Zygosaccharomyces siamensis strain) that " special strain " is somebody's turn to do in identification. But maqting type saccharomycete just understands growth and breeding only in honey density between 30%-60%, and higher than this concentration or less than this Fungal spore is found no in the honey sample of concentration --- the growth and breeding phenomenon of maqting type saccharomycete, i.e., should " special bacterium Kind " --- the honey density window of maqting type Yeast Growth breeding is 30%-60%.
Although above description of test honey can suppress the growth and breeding of fungal spore quasi-microorganism wherein, cannot kill Fungal spore;That is there is the presence of fungal spore quasi-microorganism in honey, simply no growth and breeding.So, when without60After the honey wound dressing that Co- gamma-rays radiation sterilizations are crossed is used for wound, honey wound dressing is diluted to honey by wound exudate When concentration is 30%-60%, honey wound dressing is likely to become jointly the culture medium of fungal spore with wound exudate, promotes it Growth and breeding, so as to become pathogen, new fungal infection is brought to wound.For this reason, should find without60Co- gamma-rays shines In the case of penetrating sterilizing, honey activity can be kept but also suppress the honey wound dressing of new fungal infection, therefore obtained Technical scheme.
Below in conjunction with the drawings and specific embodiments, present disclosure is further illustrated, and the present invention is made further Illustrate, but these embodiments there are any restrictions to the present invention absolutely not.Those skilled in the art are under the enlightenment of this specification to this Any variation made in inventive embodiments will be all within the scope of the present invention.
The source of used biomaterial is extensive in embodiment, and any keep on the right side of the law can be obtained with moral ethics The biomaterial taken can be replaced according to the prompting in embodiment.Method therefor is conventional side unless otherwise instructed Method.Unless otherwise instructed, the material of same names or reagent content are identical in each embodiment.
Natural honey wound dressing provided by the invention, including natural honey and nystatin, nystatin is in natural bee Final concentration of 10mg/L-50mg/L in honey.
The preparation process of the natural honey wound dressing is as follows:Nystatin is added in natural honey, to nystatin Final concentration of 10-50mg/L (concentration of nystatin is too low to influence its antimycotic effect, too high to have side effect), i.e., Obtain natural honey wound dressing;Natural honey wound dressing directly can be coated on wound during use, or coated on wound after Reusable outer dressing covers.
Wherein, brew and form after the nectar or the sweet dew of plant secretion that natural honey is bloomed by bee gathering, including extremely Few 181 kinds of materials, are a kind of supersaturated sugar juice, predominantly fructose (38%), glucose (31%), also have mineral matter, albumen, Free amino acid, enzyme and vitamin etc..The small component of other contents is also very much, such as phenolic acid, flavonoids, some enzyme (grapes Carbohydrate oxidase, catalase), amino acid.Therefore honey is in addition to antibacterial action, the various nutritional ingredients in honey Extra nutritional support can also be provided for the healing of wound.Honey can be activated and played an important role in wound healing process Macrophage, can will be not cured wound turn to hyperplasia, phase of regeneration from chronic inflammatory state.In addition honey can also promote B lymphs Cell, the mitosis of T lymphocytes and the phagocytosis of neutrophil leucocyte.Meanwhile honey can also pass through stroma cell derivative The generation of the factor -1 (stromal cell-derived factor-1, SDF-1) and promote endothelial progenitor cells to mobilize, Jin Ercan With the generation of new vessels so that wound healing.Separately it has been reported that honey promotes wound by accelerating contraction of wounds Healing, observation find that honey can substantially speed up the dermis restoration of surgical wound surface, and topical application may be to each stage of wound repair It is beneficial, so that healing acceleration process.
It is overall at present to think external application honey energy antibacterial, promote autolytic debridement, stimulate wound tissue to increase, so as to accelerate wound Healing, and dormancy wound is started healing process, anti-inflammatory activity is stimulated so as to mitigate pain, oedema and exudation rapidly.By difference The Asia honey of formula is used for the local treatment and oral medication of the various wound models of rat, no matter finding dosage height to wound Mouth healing increases beneficial to (P < 0.05), granulation tissue increase, upper dermatotome, followed by Wound Contraction, compared with control group, big agent Amount honey group hydroxyproline content increase becomes apparent from, and it is more vigorous to illustrate that collagen is formed.
Honey, which is applied to acute wounds, can provide wound healing required moist environment, prevent bacterium infection, reduce scorching Disease, oedema, exudation, promote new vessels formation, granulation tissue hyperplasia, epithelialization, accelerate wound healing, reduce cicatrization, Reduce patient pain's sense etc..It is mainly its antibacterial action in addition to the above-described advantages applied to chronic wounds.
Nystatin is a kind of more ene-type antibiotic, has conjugated polyene macrolide structure, can suppress fungi and skin moss The activity of bacterium, acts on bacterium unrestraint.Nystatin is acted on broad-spectrum antifungal, high to the antibacterial activity of Mycotoruloides, Cryptococcus neoformans, aspergillus, mucor, sporidiole bacteria, capsule tissue slurry born of the same parents bacterium, Blastomyces dermatitidis and dermatophyte are usually to it It is sensitive.Nystatin can be combined with the sterol on fungal cell membrane, cause the change of permeability of cell membrane, so that in important cells It is tolerant leakage and play antifungic action.Nystatin takes orally rear intestines and stomach and does not absorb, to blood concentration pole after common oral dose It is low, Systemic fungal infections are discharged without therapeutic effect, almost all dosage from excrement;Local topical also not by skin and glues Film absorbs.Main research Nyotran injection plays its antifungic action by being injected intravenously at present.Invention people's will Outer discovery nystatin can obtain after being assembled with natural honey not only to have been kept honey activity but also can suppress the bee of new fungal infection Sweet wound dressing, and other antifungal drugs are assembled then without this effect with natural honey.
Natural honey wound dressing provided by the invention can also include the wound dressing such as alginates, alginate dressing with auxiliary Material.
When adding alginates, the preparation process of natural honey wound dressing is:Directly by natural honey, nystatin (concentration 10-50mg/L) and alginates (final concentration is not higher than 0.5wt%) are mixed to form natural honey wound dressing.
When adding alginate dressing, the preparation process of natural honey wound dressing is as follows:Nystatin is added natural In honey, after the concentration to nystatin is 10-50mg/L, infiltrate in alginate dressing, form natural honey wound dressing; Alginate dressing is a kind of cavernous body in the form of sheets, thicker than general gauze dressing, thickness about 3mm, precious (China) purchased from health and happiness Medical supplies Co., Ltd;About per 10cm2Alginate dressing on infiltrate 5-10mL honey.
Since the alginates in alginates and alginate dressing have hygroscopicity, thickening property and into colloidality, honey can be improved The shortcomings that heat easily flows, accelerating wound healing, therefore, when use are applied directly to wound or filling wound.
Alginates are the natural polysaccharide carbohydrates extracted in seaweed, are a kind of natural macromolecular material, to human body Without any toxicity, can use safely;Absorbable 11 times of liquid equivalent to own wt;Contact wound exudate can discharge Ca2+, energy Promote the formation of thrombokinase, accelerate blood clotting process;Absorbable wound exudate, and Na occurs with sepage+/Ca2+Ion Exchange, one layer of stable network gel is formed in wound surface, building one for wound is beneficial to promote growth factor release, stimulates Cell Proliferation, improves the power of regeneration and signaling of epidermal cell, promotes wound healing, the microenvironment of tissue growth (slightly sour, Anaerobic or hypoxemia, appropriateness moistening), such microenvironment is conducive to neutrophil leucocyte and plays a role, and strengthens local bactericidal ability, drop Low infection rate.
A kind of feature wound dressing for high-absorbable energy that alginate dressing is made of alginates.The dressing touches After wound fluid, the gel of softness can be formed, preferable moist environment is provided for wound healing, promotes wound healing, alleviate Wound pain.
Add the wound dressing good airproof performance of alginates, additionally it is possible to wound is completely cut off with extraneous bacterium, harmful bacteria is consolidated Fibrous inside is scheduled on, effectively inhibits harmful bacteria to breed and reduce the chance that bacterium contacts with the surface of a wound;Alginate dressing is being hindered The water gel that discharge surface is formed effectively protects nerve ending, avoids environmental stimuli, is not easy and wound adhesion, easily removal, reduction Wound pain, not damaged non-stimulated to the surface of a wound, scar form few.
A series of natural honey wound dressings are made in the method for being prepared as described above, as shown in table 1;By a series of wounds in table 1 Mouth dressing is used for the wound that subject's yellow surface of a wound is 75%, and starting wound exudate amount is each about 10mL/d, replaces once within every 3 days Wound dressing.After natural honey wound dressing is used for wound different number of days, natural honey or nystatin can be by wound exudates Dilute, natural honey final concentration and nystatin final concentration refer respectively to the wound of the natural honey after being diluted by wound exudate in table 1 The concentration of natural honey or nystatin in mouth dressing.
Colony growth situation is observed after use, including (bacterium includes escherichia coli and Bao Mans vinegar to bacterial growth situation Sour calcium acinetobacter calcoaceticus) and fungi upgrowth situation (fungi is maqting type saccharomycete), wherein fungi upgrowth situation refers to be created with wound 10, face method takes wound exudate, and after shaking bacterium 72h in microbial growth curve detector, oily Microscopic observation fungi grows shape Condition;Using wound dressing initial stage in embodiment 2- embodiments 6 and reference examples 2- reference examples 5, it is not observed in wound exudate The growth of fungi, but germy growth;For this several groups of wound dressings, listed colony growth situation makes in table 1 With the colony growth situation in wound exudate after wound dressing 20 days.Alginate dressing area is to be completely covered wound as its area Lower limit.
The combination of the natural honey wound dressing of the present invention of table 1
By the result of embodiment 1 and comparative example 1 in table 1 as it can be seen that having used the wound of 1 natural honey wound dressing of embodiment Mouthful, the 10th day after use, the yellow surface of a wound was down to 50% by 75%, and with the appearance newly to granulate;The 19th after to use My god, wound area is by 4 × 5cm2It is contracted to 3 × 5cm2, the yellow surface of a wound is down to 25% by 50%, and the well-grown that newly granulates, carefully The growth of bacterium is also restrained.And there is no antifungal in the wound dressing of comparative example 1 --- nystatin, although using contrast The 10th day after 1 wound dressing of example, wound situation is substantially the same manner as Example 1, but the 19th day after use, hence it is evident that as can be seen that (or 4 × the 5cm of the 10th day is not being reduced with the wound area of 1 wound dressing of comparative example2), the yellow surface of a wound also no longer reduces (being still the 50% of the 10th day), and the undergrowth newly to granulate.As for the 10th day after use 1 wound dressing of comparative example, not yet There is the growth of fungi, it may be possible to which, because wound dressing can be replaced once every three days, on the one hand the growth of fungi is needs and wound The growth competition of other bacteriums, on the other hand needs the accumulation of certain time in mouth sepage, and therefore, the 19th is genius after use It was found that there is the growth of fungi in wound exudate.It follows that compared with comparative example 1, the natural bee of the present invention containing nystatin Sweet wound dressing cannot can suppress the growth of fungi in honey, so as to avoid wound from having new infection, and can save60Co- gamma-rays irradiates this step of being difficult to carry out, and also has the effect for promoting healing to wound.
Nystatin content in table 1 in embodiment 4 is higher, and wound and periwound skin can be made to produce allergy, contact The adverse reactions such as property dermatitis;And the nystatin content in embodiment 5 is relatively low, antimycotic effect can not be played, therefore, is being hindered The presence of fungi is able to observe that in mouth sepage.Without using (embodiment 6) when alginates or alginate dressing, though it can also reach anti- The effect of fungi promoting healing, but since honey has certain mobility so that wound dressing can not docile wound, when in use not It is convenient.
Reference examples 2- reference examples 5 in table 1 are to be substituted for the nystatin in natural honey wound dressing of the present invention separately A kind of common antifungal --- Fluconazole, it turns out that, with the processed wound of wound dressing of reference examples 2- reference examples 5 Mouthful, still there is the presence of fungi in its wound exudate, it is seen then that not all antifungal can have with honey collocation Antimycotic effect.
In short, the natural honey wound dressing of the present invention is when acting on wound, anti-fungal composition --- nystatin can be with Suppress the growth of fungi in honey, so as to avoid wound from having new infection, natural honey can suppress the growth of other pathogenic bacteria, And autolytic debridement and tissue growth etc. is promoted to promote wound healing.The preparation method of natural honey wound dressing of the present invention at the same time It is simple and practicable, eliminate60Co- gamma-rays irradiates this step of being difficult to carry out, additionally it is possible to reach through60The irradiation of Co- gamma-rays Fungistatic effect, is adapted to clinical widely use.
Experimental example two:
Use wound dressing (gauze size and the wound of embodiment 2, the wound dressing of reference examples 1 and reference examples 4 in table 1 Sizableness) spreading on sepage amount respectively, (24h wound exudates amount is a small amount of less than 5mL in middle amount to substantial amounts of wound;5~10mL Between be middle amount;It is a large amount of more than 10mL).Wound exudate is taken in oily Microscopic observation, discovery with 10 methods of wound surface after 72h Having does not have fungi growth in the wound exudate for having used 2 wound dressing of embodiment, use 4 wound dressing of reference examples 1 and reference examples Wound exudate in have fungi growth and breeding.As it can be seen that the natural honey wound dressing of the present invention also can without ray sterilizing Suppress the growth of fungi, prevent wound from having new infection.
Effect sepage amount is a small amount of wound in the same way, and observation is sampled after 72h, does not find but to connect in wound exudate Mould assembly saccharomycete, reason are probably that wound exudate amount is small, and natural honey is not diluted to the concentration window of 30%-60%, so Fungi does not have the phenomenon of growth and breeding.
The above is only the preferred embodiment of the present invention, it is noted that for the common skill of the art For art personnel, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications Also it should be regarded as protection scope of the present invention.

Claims (12)

1. a kind of natural honey wound dressing, it is characterised in that including honey and nystatin, the honey is without any dilute The natural honey released, the concentration of nystatin is 10-50mg/mL in the natural honey wound dressing;
Pharmaceutically acceptable auxiliary material, the auxiliary material thickener are further included, the thickener applies for alginates and/or alginates Material.
2. natural honey wound dressing according to claim 1, it is characterised in that the alginates are hindered in the natural honey Final concentration in mouth dressing is not higher than 0.5wt%.
3. natural honey wound dressing according to claim 2, it is characterised in that the alginates are sodium alginate, seaweed It is one or more of in sour potassium and calcium alginate.
4. according to any natural honey wound dressings of claim 1-3, it is characterised in that the alginate dressing is with algae Hydrochlorate is the sheet cavernous body of main component, per cm20.5-1mL natural honeys are carried on the alginate dressing and concentration is The nystatin of 10-50mg/mL.
5. natural honey wound dressing according to claim 4, it is characterised in that including natural honey, concentration 20- The nystatin and alginate dressing of 50mg/mL, nystatin and natural honey are pressed per mL natural honeys 2cm2Alginate dressing Ratio be infiltrated in the alginate dressing;
Or including natural honey, concentration is the nystatin and alginate dressing of 20-40mg/mL, nystatin and natural honey By every mL natural honeys 1.5cm2The ratio of alginate dressing is infiltrated in the alginate dressing.
6. natural honey wound dressing according to claim 5, it is characterised in that including natural honey, concentration 20mg/mL Nystatin and alginate dressing, nystatin and natural honey are pressed per mL natural honeys 1.5cm2The ratio of alginate dressing It is infiltrated in the alginate dressing.
7. natural honey wound dressing according to claim 6, it is characterised in that by natural honey, concentration 20-50mg/ The nystatin of mL and the alginates of 2wt%-5wt% mix;
Or by natural honey, concentration is that the nystatin of 20-40mg/mL and the alginates of 3wt%-5wt% mix.
8. natural honey wound dressing according to claim 7, it is characterised in that by natural honey, concentration is 20mg/mL's The alginates of nystatin and 4wt% mix.
A kind of 9. method for preparing any natural honey wound dressings of claim 1-8, it is characterised in that by nystatin It is uniformly mixed with natural honey, the concentration of nystatin is 10-50mg/L after mixing, that is, obtains natural honey wound dressing.
10. method according to claim 9, it is characterised in that nystatin and natural honey are infiltrated into algae after mixing Hydrochlorate dressing, obtains natural honey wound dressing, the usage amount of the alginate dressing is 1-2cm2Alginate dressing/mL is natural Honey.
11. method according to claim 9, it is characterised in that natural honey, nystatin and alginates are uniformly mixed, Obtain natural honey wound dressing;Wherein, the concentration of nystatin is 10-50mg/L after mixing, and the concentration of alginates is not higher than 0.5wt%.
12. any natural honey wound dressings of claim 1-8 or any the methods of claim 9-11 are prepared Application of the natural honey wound dressing in treat wound infection, chronic wounds or acute wounds medicine is prepared.
CN201510617897.7A 2015-09-24 2015-09-24 A kind of natural honey wound dressing and preparation method and application Expired - Fee Related CN105169464B (en)

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WO2021236024A1 (en) 2020-05-21 2021-11-25 Ptt Public Company Limited Preparation of biocellulose wound dressing comprising honey as an antimicrobial active ingredient
CN112336914A (en) * 2020-10-28 2021-02-09 广东泰宝医疗科技股份有限公司 Sodium alginate composite gel and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1409636A (en) * 1999-12-09 2003-04-09 威凯托陵科有限公司 Use of honey in medical dressings
CN1444488A (en) * 2000-06-30 2003-09-24 菲利普·罗伊·卡斯基 Use and improvement of honey in wound dressings
CN202236501U (en) * 2011-09-25 2012-05-30 三峡大学第一临床医学院 Honey dressing for exchanging medicament of infective wound
CN103623007A (en) * 2013-10-25 2014-03-12 王冬梅 Externally-applied ointment for treating ringworm of scalp and preparation and application methods thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007070694A2 (en) * 2005-12-14 2007-06-21 Zars, Inc. Spray-on formulations and methods for dermal delivery of drugs

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1409636A (en) * 1999-12-09 2003-04-09 威凯托陵科有限公司 Use of honey in medical dressings
CN1444488A (en) * 2000-06-30 2003-09-24 菲利普·罗伊·卡斯基 Use and improvement of honey in wound dressings
CN202236501U (en) * 2011-09-25 2012-05-30 三峡大学第一临床医学院 Honey dressing for exchanging medicament of infective wound
CN103623007A (en) * 2013-10-25 2014-03-12 王冬梅 Externally-applied ointment for treating ringworm of scalp and preparation and application methods thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
大蒜蜂蜜制霉菌素油膏治疗新生儿红臀;陈娅兰;《中国民间疗法》;19991031(第10期);第15页 *

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