CN104069250A - Traditional Chinese medicinal preparation for treating skin tinea and preparation method thereof - Google Patents
Traditional Chinese medicinal preparation for treating skin tinea and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicinal preparation for treating skin tinea. The preparation is characterized by comprising the following raw materials in parts by weight: 90-150 parts of golden larch bark, 80-150 parts of cortex phellodendri chinensis, 50-80 parts of cortex dictamni, 30-90 parts of radix sophorae flavescentis, 40-70 parts of paniculate swallowwort root, 40-90 parts of pomegranate bark, 50-80 parts of flos daturae, 25-65 parts of semen hygrophilae, 25-50 parts of broom cypress fruit and 20-50 parts of camphor. The traditional Chinese medicinal preparation is prepared in the following steps: preparing a traditional Chinese medicinal formula raw material extract by adopting a percolation method, and preparing liniment, gel, ointment or spray as required. According to the traditional Chinese medicinal preparation, polysaccharide components in the percolated decoction dregs are extracted and used as a gel substrate, so that excellent biocompatibility and degradability can be given to the formula extract, the drug component is effectively promoted to infiltrate into skin surface and contacted with fungus, and the curative effect of the medicine can be improved.
Description
Technical field
The present invention relates to a kind of application field for preparing medicines, relate in particular to a kind of Chinese medicine preparation that is used for the treatment of dermatophytosis and preparation method thereof.
Background technology
Dermatophytosis is cutaneous fungal infection disease, pathogenic germ is dermatophytosis, mainly comprise trichophyton, Microsporon and Epidermophyton, its common feature is close keratoprotein, invades skin, hair, the deck of humans and animals, causes infection, the fungus that infects the mankind environment that mainly comes from the outside, route of infection can be contact, sucks or eats etc., is a kind of more difficult radical cure, the skin infectious disease that is easy to again recurrence, and clinical manifestation is tinea corporis, tinea cruris, tinea pedis, tinea unguium, tinea capitis etc.Traditional Chinese medical science choosing diagnosis, tinea is by being subject to pathogenic wind toxic outward, cohesion skin, very skin can not make moist, or because wind and cold is outward, battalion defends imbalance, or a wind heat intrusion hair key, blood is dry more for a long time, or Chong and Ren channel disorder, nutrient blood loss, wind syndrome due to blood-deficiency dryness-transformation etc. causes skin mistake and supports, or is invaded by rheumatism, stay in space between skin and muscles, or occupy wetland for a long time, water slurry dipping, strongly fragrant in skin, or become soaked with sweat because antiperspirant doctor is wet, wash rice skin flooded, be subject to again Exposure to Sunlight, heat-damp in summer is invaded stain hair key, forms primary disease.Modern medicine thinks, tinea is by due to mycete, and fungal species is various, and the overwhelming majority can not be caused a disease, and wherein sub-fraction is conditioned pathogen, and skin, mucosa, intestinal that can be present in people etc. is located.Under normal circumstances, between each flora, influence each other, restriction mutually, balance metabolism.But because life-time service antibiotic can cause dysbacteriosis in body, damaged when human body skin, when resistance declines, disease cause fungi is amount reproduction, invades skin, subcutaneous tissue and cause the generation of tinea.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine preparation that is used for the treatment of tinea and preparation method thereof, can infiltrate fast skin, accelerate sterilization speed, thereby shorten treatment time, accelerate therapeutic effect, can comparatively fast treat tinea class disease, recurrence probability is less, is applicable to multiple ill crowd.
Technical scheme provided by the invention is as follows:
A Chinese medicine preparation that is used for the treatment of dermatophytosis, by the raw material of following components by weight percent, made:
Cortex Pseudolaricis 90-150, Cortex Phellodendri 80-150, Cortex Dictamni 50-80, Radix Sophorae Flavescentis 30-90, Radix Cynanchi Paniculati 40-70, Pericarpium Granati 40-90, Flos Daturae 50-80, seed of Hygrophila saxatilis Ridl. 25-65, Fructus Kochiae 25-50 and Camphora 20-50.
And prepared by following methods:
Step 1, is ground into 120 object fine powders by Cortex Phellodendri;
Step 2, is broken into coarse powder by medical material mixed powders such as Cortex Pseudolaricis, Cortex Dictamni, Radix Sophorae Flavescentis, Radix Cynanchi Paniculati, Flos Daturae, Pericarpium Granati, the Fructus Kochiae, seed of Hygrophila saxatilis Ridl.;
Step 3, the ethanol that is 70-80% by salicylic acid and volumetric concentration by weight volume ratio are 1.0: the ratio preparation salicylic acid alcoholic solution of 99.0-98.5;
Step 4, above-mentioned steps one and step 2 medicated powder are mixed, with the moistening of above-mentioned salicylic acid alcoholic solution, then the mixed powder after moistening is packed in percolator, the salicylic acid alcoholic solution dipping 40-50 hour that use is doubly measured with respect to medicated powder weight 5-7, percolation, collect the liquid of just filtering and filter liquid as extracting solution with continuous, retain medicinal residues, standby;
Step 5, adopts different preparation technologies extracting solution to be prepared into medicinal liquid or the clear paste of suitable concn, then adds respectively other excipient and Camphora according to preparation characteristic, is prepared into liniment, gel, ointment or spray.
Wherein, the raw material using is taken according to following parts by weight: Cortex Pseudolaricis 90-150, Cortex Phellodendri 80-150, Cortex Dictamni 50-80, Radix Sophorae Flavescentis 30-90, Radix Cynanchi Paniculati 40-70, Pericarpium Granati 40-90, Flos Daturae 50-80, seed of Hygrophila saxatilis Ridl. 25-65, Fructus Kochiae 25-50 and Camphora 20-50.
Preferably, described liniment preparation method is as follows:
1) the continuous diacolation liquid recycling ethanol first described step 4 being obtained is also concentrated into the clear paste that relative density is 1.15-1.25, mix homogeneously with the liquid of just filtering again, standing 24 hours, get supernatant, add the PEG400 with respect to supernatant weight 10%-25%, put into autoclave, the pressure of fast lifting autoclave is to 100-200Mpa, reaction under high pressure 8-20 minute, when reaction under high pressure, the solution in reactor is carried out to high speed shear stirring simultaneously, filter, by filtrate centrifugalize, retain filtrate;
2) Camphora is used to 95% dissolve with ethanol, is added described step 1) filtrate in, then add 70%-80% ethanol, stirring and evenly mixing, makes liniment.
Utilize the effect of supertension, make the protein denaturation in ingredient, enzyme deactivation, membranolysis, in thalline, composition is revealed, microbial cells is destroyed and dead, extended the shelf-life of medicine, not needing to add other one-tenth assigns to extend the shelf life, also simplified technological process simultaneously, reduce energy consumption, reduce costs, the PEG400 simultaneously adding has good biocompatibility, can be used as the carrier of effective ingredient, under high speed shear effect, increase motion frequency and the speed of material molecule, reduce the particle diameter of PEG400, increase penetration capacity, improve drug-eluting speed, medicine can better be absorbed by the body, PEG400 can also effectively regulate the viscosity of made liniment, increase the time of contact of medicine and skin.
Preferably, described gel is prepared as follows:
1) by the medicinal residues Distillation recovery ethanol in described step 4, then add the water doubly with respect to medicinal residues w/v 6-8, decoct 1-2 hour, filtered while hot, retains filtrate; 2-4 time repeatedly, merging filtrate;
2) by step 1) gained filtrate is concentrated into the clear paste that relative density is 1.15-1.25, add the 65%-80% alcoholic solution doubly with respect to clear paste w/v 3-6, at 5-8 ℃, the standing 45-50 hour of cold preservation, then gets lower sediment high speed centrifugation, collecting precipitation, obtains water soluble polysaccharide;
3) water soluble polysaccharide is added appropriate distilled water stir into colloid, add alkaline solution to regulate the pH to 7.5-8.6 of water soluble polysaccharide, adding parts by weight is the cross-linking agent of 10-30, under stirring condition, slowly add parts by weight to be standing 10-20 hour after the wetting agent of 5-15 and the mixture of the Tween 80 that parts by weight are 3-10 add appropriate distilled water to stir, standby;
4) the first liquid of filtering first described step 4 being obtained, the continuous liquid of filtering merge, reclaim ethanol and be concentrated into the thick paste that relative density is 1.25-1.35, by Camphora 95% dissolve with ethanol, then add in thick paste, stir, then add step 3 several times) in products therefrom, then under stirring condition, add buffer solution to regulate pH to 5.5-6.8, fully stir, obtain required gel.
The present invention is by reclaiming the polysaccharide component in medicinal residues, use polysaccharide component regulates the viscosity of made gel, the time of contact of prolong drug and skin, the action time of effective ingredient in control medicine, improve the fungistatic effect of gained medicine of the present invention, thereby reached the object that improves curative effect.And gained polysaccharide component also has non-oxidizability, prevent in medication process that oxidized causing of active drug composition lessens the curative effect.
Preferably, alkaline solution described step 3) is selected from a kind of in sodium hydroxide, calcium hydroxide, ethylenediamine;
Preferably, cross-linking agent described step 3) is selected from malonic acid aminobenzene boric acid and/or sodium polyacrylate; The interpolation of cross-linking agent, forms macromolecule network system with water soluble polysaccharide, effective ingredient material can be coated wherein, has greatly increased the concentration of effective ingredient, reduces use amount.And the wetting agent in wetting agent and effective ingredient are evenly cross-linked wherein, keep the stability of medicine, avoid the too fast loss of Moisture factor in medication process to cause drug failure.
Preferably, wetting agent described step 3) is selected from glycerol and/or hyaluronic acid.Glycerol and the hyaluronic acid horny layer that can not only deeply penetrate to the skin, effectively improves the Nutrition and Metabolism of skin, makes skin tender, smooth, increase elasticity, or a kind of good Percutaneous absorption enhancer, promote ingredient to infiltrate skin surface, improve therapeutic effect, shorten treatment time.
Preferably, buffer solution described step 4) is selected from sodium bicarbonate-carbonic acid or sodium acetate-acetic acid.Adopt buffer solution, effectively control the excursion of pH value of solution, avoid affecting because pH is too high or too low effect of drugs.
The present invention is ground into fine powder by Cortex Phellodendri, can significantly improve the wherein extraction ratio of effective ingredient berberine; Use salicylic acid adjust pH, can promote the extraction of the effective ingredient such as alkaloid in medicine, can promote skin permeation of drugs horny layer and hair follicle, impel medicine to absorb fast simultaneously.Medicine tool heat clearing and damp drying of the present invention, the effect of killing parasites for relieving itching, antifungic action is strong, evident in efficacy for skin tineas such as tinea pedis, the tinea manuum, tinea corporis, tinea cruris.
Wherein, the made liniment of the present invention utilizes the effect of supertension, make the protein denaturation in ingredient, enzyme deactivation, membranolysis, in thalline, composition is revealed, microbial cells is destroyed and dead, extended the shelf-life of medicine, not needing to add other one-tenth assigns to extend the shelf life, also simplified technological process simultaneously, reduce energy consumption, reduce costs, the PEG400 simultaneously adding has good biocompatibility, can be used as the carrier of effective ingredient, under high speed shear effect, increase motion frequency and the speed of material molecule, reduce the particle diameter of PEG400, increase penetration capacity, improve drug-eluting speed, medicine can better be absorbed by the body, PEG400 can also effectively regulate the viscosity of made liniment, increase the time of contact of medicine and skin.
Polysaccharide component in the present invention in made gel drug of choice raw material is as gel base material, can give biocompatibility and degradability that formula extracting solution of the present invention is good, and the particle diameter of effective ingredient medicine is very little, effectively promoting ingredient to infiltrate skin surface contacts with fungus, shorten treatment time, improve curative effect.Meanwhile, the network structure that cross-linking agent and polysaccharide component form, connects the Moisture factor in effective component drugs thing and wetting agent, control water loss speed, prolong drug action time.From extracting polysaccharide through the medicinal residues of alcohol extraction, rationally utilize garbage, reduce preparation cost, improve salvage value, and method is simple to operation, the product obtaining is also very stable.
The specific embodiment
Embodiment 1
A Chinese medicine preparation that is used for the treatment of dermatophytosis, by the raw material of following components by weight percent, made:
Cortex Pseudolaricis 120g, Cortex Phellodendri 150g, shaggy-fruited dittany 50g, Radix Sophorae Flavescentis 30g, Radix Cynanchi Paniculati 70g, Pericarpium Granati 40g, Flos Daturae 50g, seed of Hygrophila saxatilis Ridl. 25g, Fructus Kochiae 30g and Camphora 20g.
And comprise the following steps:
Step 1, is ground into 120 object fine powders by Cortex Phellodendri;
Step 2, is broken into coarse powder by medical material mixed powders such as Cortex Pseudolaricis, Cortex Dictamni, Radix Sophorae Flavescentis, Radix Cynanchi Paniculati, Flos Daturae, Pericarpium Granati, the Fructus Kochiae, seed of Hygrophila saxatilis Ridl.;
Step 3, by salicylic acid and the 70-80% ethanol ratio that volume ratio is 1.0: 99.0 by weight preparation salicylic acid alcoholic solution;
Step 4, above-mentioned steps one and step 2 medicated powder are mixed, with the moistening of above-mentioned salicylic acid alcoholic solution, then the mixed powder after moistening is packed in percolator, the salicylic acid alcoholic solution dipping that use is doubly measured for 5-7 with respect to medicated powder w/v 40 hours, percolation, collects and just filters liquid and continuously filter liquid as extracting solution, retain medicinal residues, standby;
Step 5, adopts different preparation technologies extracting solution to be prepared into medicinal liquid or the clear paste of suitable concn, then adds respectively other excipient and Camphora according to preparation characteristic, is prepared into liniment, gel, ointment or spray.
Described liniment preparation method is as follows:
1) the continuous diacolation liquid recycling ethanol first described step 4 being obtained to be concentrated into relative density be 1.15 clear paste, mix homogeneously with the liquid of just filtering again, standing 24 hours, get supernatant, add the PEG400 with respect to supernatant weight 10%, put into autoclave, the pressure of fast lifting autoclave is to 100Mpa, reaction under high pressure 8 minutes, when reaction under high pressure, the solution in reactor is carried out to high speed shear stirring simultaneously, filter, by filtrate centrifugalize, retain filtrate;
2) Camphora is used to 95% dissolve with ethanol, is added described step 1) filtrate in, then add 70% ethanol, stirring and evenly mixing, makes 1000ml liniment.
Described gel preparation method is as follows:
1) by the medicinal residues Distillation recovery ethanol in described step 4, then add the water with respect to 6 times of medicinal residues w/vs, decoct 1 hour, filtered while hot, retains filtrate; 3 times repeatedly, merging filtrate;
2) by step 1) to be concentrated into relative density be 1.15 clear paste to gained filtrate, add 65% alcoholic solution with respect to 3 times of clear paste w/vs, at 5 ℃, cold preservation is standing 45 hours, then gets lower sediment high speed centrifugation, collecting precipitation, obtains water soluble polysaccharide;
3) water soluble polysaccharide is added appropriate distilled water stir into colloid, add sodium hydroxide solution to regulate the pH to 7.5 of water soluble polysaccharide, add sodium polyacrylate 10g, under stirring condition, slowly add the mixture of hyaluronic acid 5g and Tween 80 3g to add appropriate distilled water to stir latter standing 10 hours, standby;
4) the first liquid of filtering first described step 4 being obtained, the continuous liquid of filtering merge, reclaiming ethanol and being concentrated into relative density is 1.25 thick paste, by Camphora 95% dissolve with ethanol, then add in thick paste, stir, then divide 3 times to add step 3) in products therefrom, then under stirring condition, add buffer solution to regulate pH to 5.5, fully stir, obtain required gel.
Embodiment 2
A Chinese medicine preparation that is used for the treatment of dermatophytosis, by the raw material of following components by weight percent, made:
Cortex Pseudolaricis 100g, Cortex Phellodendri 150g, Cortex Dictamni 60g, Radix Sophorae Flavescentis 40g, Radix Cynanchi Paniculati 55g, Pericarpium Granati 90g, Flos Daturae 60g, seed of Hygrophila saxatilis Ridl. 33g, Fructus Kochiae 40g and Camphora 30g.
And comprise the following steps:
Step 1, is ground into 120 object fine powders by Cortex Phellodendri;
Step 2, is broken into coarse powder by medical material mixed powders such as Cortex Pseudolaricis, Cortex Dictamni, Radix Sophorae Flavescentis, Radix Cynanchi Paniculati, Flos Daturae, Pericarpium Granati, the Fructus Kochiae, seed of Hygrophila saxatilis Ridl.;
Step 3, by salicylic acid and the 80% ethanol ratio that volume ratio is 1.0: 98.5 by weight preparation salicylic acid alcoholic solution;
Step 4, above-mentioned steps one and step 2 medicated powder are mixed, with the moistening of above-mentioned salicylic acid alcoholic solution, then the mixed powder after moistening is packed in percolator, the salicylic acid alcoholic solution that use is 6 times of amounts with respect to medicated powder w/v floods 45 hours, and percolation is collected the liquid of just filtering and filtered liquid as extracting solution with continuous, retain medicinal residues, standby;
Step 5, adopts different preparation technologies extracting solution to be prepared into medicinal liquid or the clear paste of suitable concn, then adds respectively other excipient and Camphora according to preparation characteristic, is prepared into liniment, gel, ointment or spray.
Described liniment preparation method is as follows:
1) the continuous diacolation liquid recycling ethanol first described step 4 being obtained to be concentrated into relative density be 1.20 clear paste, mix homogeneously with the liquid of just filtering again, standing 24 hours, get supernatant, add the PEG400 with respect to supernatant weight 20%, put into autoclave, the pressure of fast lifting autoclave is to 150Mpa, reaction under high pressure 15 minutes, when reaction under high pressure, the solution in reactor is carried out to high speed shear stirring simultaneously, filter, by filtrate centrifugalize, retain filtrate;
2) Camphora is used to 95% dissolve with ethanol, is added described step 1) filtrate in, then add appropriate 80% ethanol, stirring and evenly mixing, makes 1000ml liniment.
Described gel preparation method is as follows:
1) by the medicinal residues Distillation recovery ethanol in described step 4, then add the water with respect to 7 times of medicinal residues w/vs, decoct 2 hours, filtered while hot, retains filtrate; 4 times repeatedly, merging filtrate;
2) by step 1) to be concentrated into relative density be 1.20 clear paste to gained filtrate, add 75% alcoholic solution with respect to 5 times of clear paste w/vs, at 7 ℃, cold preservation is standing 48 hours, then gets lower sediment high speed centrifugation, collecting precipitation, obtains water soluble polysaccharide;
3) water soluble polysaccharide is added appropriate distilled water stir into colloid, add aqua calcis to regulate the pH to 8 of water soluble polysaccharide, add malonic acid aminobenzene boric acid 15g, under stirring condition, slowly add the mixture of hyaluronic acid 6g, ethoxy ammonium carbonate 6g and Tween 80 8g to add appropriate distilled water to stir latter standing 15 hours, standby;
4) the first liquid of filtering first described step 4 being obtained, the continuous liquid of filtering merge, reclaiming ethanol and being concentrated into relative density is 1.3 thick paste, by appropriate 95% dissolve with ethanol for Camphora, then add in thick paste, stir, then divide 4 times to add step 3) in products therefrom, then under stirring condition, add buffer solution to regulate pH to 6, fully stir, obtain required gel.
Embodiment 3
A Chinese medicine preparation that is used for the treatment of dermatophytosis, by the raw material of following components by weight percent, made:
Cortex Pseudolaricis 150g, Cortex Phellodendri 130g, Cortex Dictamni 50g, Radix Sophorae Flavescentis 30g, Radix Cynanchi Paniculati 70g, Pericarpium Granati 40g, Flos Daturae 80g, seed of Hygrophila saxatilis Ridl. 25g, Fructus Kochiae 50g and Camphora 20g.
And comprise the following steps:
Step 1, is ground into 120 object fine powders by Cortex Phellodendri;
Step 2, is broken into coarse powder by medical material mixed powders such as Cortex Pseudolaricis, Cortex Dictamni, Radix Sophorae Flavescentis, Radix Cynanchi Paniculati, Flos Daturae, Pericarpium Granati, the Fructus Kochiae, seed of Hygrophila saxatilis Ridl.;
Step 3, by salicylic acid and the 75% ethanol ratio that volume ratio is 1.0: 99.0 by weight preparation salicylic acid alcoholic solution;
Step 4, above-mentioned steps one and step 2 medicated powder are mixed, with the moistening of above-mentioned salicylic acid alcoholic solution, then the mixed powder after moistening is packed in percolator, use is flooded 45 hours with respect to the salicylic acid alcoholic solution of 7 times of amounts of medicated powder w/v, and percolation is collected the liquid of just filtering and filtered liquid as extracting solution with continuous, retain medicinal residues, standby;
Step 5, adopts different preparation technologies extracting solution to be prepared into medicinal liquid or the clear paste of suitable concn, then adds respectively other excipient and Camphora according to preparation characteristic, is prepared into liniment, gel, ointment or spray.
Described liniment preparation method is as follows:
1) the continuous diacolation liquid recycling ethanol first described step 4 being obtained to be concentrated into relative density be 1.25 clear paste, mix homogeneously with the liquid of just filtering again, standing 24 hours, get supernatant, add the PEG400 with respect to supernatant weight 25%, put into autoclave, the pressure of fast lifting autoclave is to 200Mpa, reaction under high pressure 20 minutes, when reaction under high pressure, the solution in reactor is carried out to high speed shear stirring simultaneously, filter, by filtrate centrifugalize, retain filtrate;
2) Camphora is used to 95% dissolve with ethanol, is added described step 1) filtrate in, then add appropriate 80% ethanol, stirring and evenly mixing, makes 1000ml liniment.
Described gel preparation method is as follows:
1) by the medicinal residues Distillation recovery ethanol in described step 4, then add the water with respect to 8 times of medicinal residues w/vs, decoct 2 hours, filtered while hot, retains filtrate; 4 times repeatedly, merging filtrate;
2) by step 1) to be concentrated into relative density be 1.25 clear paste to gained filtrate, add 65% alcoholic solution with respect to 6 times of clear paste w/vs, at 8 ℃, cold preservation is standing 50 hours, then gets lower sediment high speed centrifugation, collecting precipitation, obtains water soluble polysaccharide;
3) water soluble polysaccharide is added appropriate distilled water stir into colloid, add ethylenediamine solution to regulate the pH to 8.6 of water soluble polysaccharide, add malonic acid aminoboronic acid 15g, sodium polyacrylate 15g, under stirring condition, slowly add the mixture of glycerol 15g and Tween 80 10g to add appropriate distilled water to stir latter standing 20 hours, standby;
4) the first liquid of filtering first described step 4 being obtained, the continuous liquid of filtering merge, reclaiming ethanol and being concentrated into relative density is 1.35 thick paste, by appropriate 95% dissolve with ethanol for Camphora, then add in thick paste, stir, then divide 5 times to add step 3) in products therefrom, then under stirring condition, add buffer solution to regulate pH to 6.8, fully stir, obtain required gel.
The In vitro Bactericidal Experiments that is used for the treatment of the Chinese medicine preparation of dermatophytosis
1, liniment In Vitro Anti tinea bacterium test
(1) extracorporeal bacteria inhibitor test
Get some, test tube after sterilizing, with the liniment obtaining in sabouraud culture medium dilution embodiment, make that its ultimate density is respectively 80,20,5,1,0.25mg/ml, every test tube adds 1 * 10
3individual fungus/ml bacterium liquid 0.1ml, is placed in 25 ℃ of incubators and cultivates 10d, observes and has or not tinea bacteria growing, and the minimum inhibitory concentration (MIC) that the highest liniment extension rate of take without tinea bacteria growing be this liniment sets up culture medium contrast and normal saline to contrast simultaneously.The liniment configuring in other embodiment according to above method carries out antibacterial tests.
(2) extracorporeal disinfecting test
Get the culture of not long bacterium more than minimum inhibitory concentration, respectively transferred species on agar plate 25 ℃ cultivate 10d, observe and have or not tinea bacteria growing, the minimum bactericidal concentration (MBC) that the concentration of take without the highest extension rate of the liniment of tinea bacteria growing is this liniment.
With viable bacteria technology law, measure the sterilizing time of liniment.Get the liniment of the embodiment 1 of the concentration that reduces by half, every test tube 3ml, the bacterium liquid 0.2ml having diluted is inoculated in to liniment in vitro, and timing immediately, respectively at 10,30, during 60min, taking out 0.1ml is placed in aseptic plate, and with the husky Bao Shi agar of 1ml, make pour plate immediately, cultivate 10d, then carry out colony counting for 25 ℃.Result of the test is as table 1:
Table 1
Note: "+" expression strain is not killed, "-" expression strain is killed.
2, the bacteriostatic test of gel
(1) by microbionation in fresh MH body fluid culture medium, 35 ℃ of overnight incubation, then bacterium liquid is proofreaied and correct to 0.5 Maxwell bacterial concentration than turbid standard by fresh MH body fluid culture medium, by MH body fluid culture medium, by 1: 100, dilute again, then in every test tube, add 1ml, then add product of the present invention, cultivate 24 hours for 35 ℃, using aseptic culture medium as blank, and the minimum pipe of concentration that relatively antibacterial does not grow is minimal inhibitory concentration simultaneously.By embodiment 1-3 obtain product of the present invention according to above-mentioned steps to trichophyton, oidium schoenleinii, white read post bacterium, Cryptococcus histolyticus is tested, result of the test is as table 2:
Table 2
As can be seen from Table 1 and Table 2, gained Chinese medicine preparation of the present invention has antibacterial, bactericidal action significantly to easily causing the strain of dermatophytosis, low concentration just can suppress the Growth and reproduction of strain, and sterilizing time is fast, and within a short period of time can be fast by strain deactivation.In sum, medicine tool heat clearing and damp drying of the present invention, the effect of killing parasites for relieving itching, antifungic action is strong, evident in efficacy for skin tineas such as tinea pedis, the tinea manuum, tinea corporis, tinea cruris.
3, the liniment of gained liniment of the present invention, gel and market being sold carries out bacteriostatic test
Described gel is tested according to the bacteriostatic test of above-mentioned gel, be labeled as group 1; Described liniment is tested according to the bacteriostatic test of above-mentioned liniment, is labeled as respectively group 2, group 3, wherein organizes 2 for gained liniment of the present invention, the liniment that group 3 is sold for market.Simultaneously to trichophyton, oidium schoenleinii, white read post bacterium, Cryptococcus histolyticus is tested, the result obtaining is as following table 3:
Table 3
Organize as can be seen from Table 31 and be obviously better than organizing 3 with the fungistatic effect of group 2, and the fungistatic effect of group 1 compares with group 2, group 1 is better than organizing 2.Group 1 is gained gel of the present invention, group 2 is gained liniment of the present invention, wherein, polysaccharide component in gel drug of choice raw material of the present invention, as gel base material, can be given biocompatibility and degradability that formula extracting solution of the present invention is good, has also increased the viscosity of made gel, the time of contact of prolong drug and skin, control the action time of effective ingredient in medicine, improved the fungistatic effect of gained medicine of the present invention, thereby reach the object that improves curative effect.And gained polysaccharide component also has oxidisability, prevent in medication process that oxidized causing of active drug composition lessens the curative effect; Gained liniment of the present invention, utilizes supertension high speed shear technology, and making formula material extracting solution is small particle diameter solution, has improved the penetrating power of ingredient, accelerates the time of contact of medicine and fungus, has improved therapeutic effect.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other modification, therefore do not deviating under the general concept that claim and equivalency range limit, the present invention is not limited to shown here specific detail.
Claims (8)
1. a Chinese medicine preparation that is used for the treatment of dermatophytosis, is characterized in that, described Chinese medicine preparation is made by the raw material of following parts by weight:
Cortex Pseudolaricis 90-150, Cortex Phellodendri 80-150, Cortex Dictamni 50-80, Radix Sophorae Flavescentis 30-90, Radix Cynanchi Paniculati 40-70, Pericarpium Granati 40-90, Flos Daturae 50-80, seed of Hygrophila saxatilis Ridl. 25-65, Fructus Kochiae 25-50 and Camphora 20-50.
2. be used for the treatment of as claimed in claim 1 a preparation method for the Chinese medicine preparation of dermatophytosis, it is characterized in that, described preparation method comprises the following steps:
Step 1, Cortex Phellodendri is ground into 120 object fine powders;
Step 2, Cortex Pseudolaricis, Cortex Dictamni, Radix Sophorae Flavescentis, Radix Cynanchi Paniculati, Flos Daturae, Pericarpium Granati, the Fructus Kochiae, seed of Hygrophila saxatilis Ridl. mixed powder are broken into coarse powder;
Step 3, the ethanol that is 70-80% by salicylic acid and volumetric concentration by weight volume ratio are 1.0: the ratio preparation salicylic acid alcoholic solution of 99.0-98.5;
Step 4, above-mentioned steps one and step 2 medicated powder are mixed, with the moistening of above-mentioned salicylic acid alcoholic solution, then the mixed powder after moistening is packed in percolator, the salicylic acid alcoholic solution dipping 40-50 hour that use is doubly measured with respect to medicated powder weight 5-7, percolation, collect the liquid of just filtering and filter liquid as extracting solution with continuous, retain medicinal residues, standby;
Step 5, extracting solution is prepared into medicinal liquid or the clear paste of suitable concn, then adds respectively excipient and Camphora, be prepared into liniment, gel, ointment or spray;
Wherein, the raw material using is taken according to following parts by weight: Cortex Pseudolaricis 90-150, Cortex Phellodendri 80-150, Cortex Dictamni 50-80, Radix Sophorae Flavescentis 30-90, Radix Cynanchi Paniculati 40-70, Pericarpium Granati 40-90, Flos Daturae 50-80, seed of Hygrophila saxatilis Ridl. 25-65, Fructus Kochiae 25-50 and Camphora 20-50.
3. be used for the treatment of as claimed in claim 2 the preparation method of the Chinese medicine preparation of dermatophytosis, it is characterized in that, described liniment preparation method is as follows:
1) the continuous diacolation liquid recycling ethanol first described step 4 being obtained is also concentrated into the clear paste that relative density is 1.15-1.25, mix homogeneously with the liquid of just filtering again, standing 24 hours, get supernatant, add the PEG400 with respect to supernatant weight 10%-25%, put into autoclave, promote the pressure of autoclave to 100-200Mpa, reaction under high pressure 8-20 minute, when reaction under high pressure, the solution in reactor is carried out to shear agitation simultaneously, filter, by filtrate centrifugalize, retain filtrate;
2) Camphora is used to 95% dissolve with ethanol, is added described step 1) filtrate in, then add 70%-80% ethanol, stirring and evenly mixing, makes liniment.
4. be used for the treatment of as claimed in claim 2 the preparation method of the Chinese medicine preparation of dermatophytosis, it is characterized in that, described gel is prepared as follows:
1) by the medicinal residues Distillation recovery ethanol in described step 4, then add the water doubly with respect to medicinal residues weight 6-8, decoct 1-2 hour, filtered while hot, retains filtrate; 2-4 time repeatedly, merging filtrate;
2) by step 1) gained filtrate is concentrated into the clear paste that relative density is 1.15-1.25, add the 65%-80% alcoholic solution doubly with respect to clear paste weight 3-6, the standing 45-50 hour of cold preservation at 5-8 ℃, then gets lower sediment centrifugal, collecting precipitation, obtains water soluble polysaccharide;
3) add distilled water to stir into colloid water soluble polysaccharide, add alkaline solution to regulate the pH to 7.5-8.6 of water soluble polysaccharide, adding parts by weight is the cross-linking agent of 10-30, under stirring condition, slowly add parts by weight to be standing 10-20 hour after the wetting agent of 5-15 and the mixture of the Tween 80 that parts by weight are 3-10 add distilled water to stir, standby;
4) the first liquid of filtering first described step 4 being obtained, the continuous liquid of filtering merge, reclaim ethanol and be concentrated into the thick paste that relative density is 1.25-1.35, by appropriate 95% dissolve with ethanol for Camphora, then add in thick paste, stir, then add step 3 several times) in products therefrom, then under stirring condition, add buffer solution to regulate pH to 5.5-6.8, fully stir, obtain required gel.
5. be used for the treatment of as claimed in claim 4 the preparation method of the Chinese medicine preparation of dermatophytosis, it is characterized in that, described step 3) alkaline solution be selected from a kind of in sodium hydroxide solution, aqua calcis and ethylenediamine solution.
6. be used for the treatment of as claimed in claim 4 the preparation method of the Chinese medicine preparation of dermatophytosis, it is characterized in that, described step 3) cross-linking agent be selected from malonic acid aminobenzene boric acid and/or sodium polyacrylate.
7. be used for the treatment of as claimed in claim 4 the preparation method of the Chinese medicine preparation of dermatophytosis, it is characterized in that, described step 3) wetting agent be selected from glycerol and/or hyaluronic acid.
8. be used for the treatment of as claimed in claim 4 the preparation method of the Chinese medicine preparation of dermatophytosis, it is characterized in that, described step 4) buffer solution be selected from sodium bicarbonate-carbonate buffer solution or sodium acetate-hac buffer.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666549A (en) * | 2015-03-29 | 2015-06-03 | 淄博夸克医药技术有限公司 | Externally applied medicament for treating tinea corporis |
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2014
- 2014-06-30 CN CN201410307819.2A patent/CN104069250A/en active Pending
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| 何琪莹: ""局部药用凝胶剂的研究新进展和新应用"", 《中国药物与临床》 * |
| 唐欣昀: "《微生物学》", 30 September 2009 * |
| 邵显会: ""关于药用植物多糖提取方法的研究"", 《生物技术世界》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104666549A (en) * | 2015-03-29 | 2015-06-03 | 淄博夸克医药技术有限公司 | Externally applied medicament for treating tinea corporis |
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Address after: 530023 No. 8-1, Kunlun Avenue, Nanning, the Guangxi Zhuang Autonomous Region Applicant after: Guangxi Baiyun Mountain Ying Kang Pharmaceutical Co., Ltd. Address before: 530023 No. 193, Gang Gang Road, Nanning, the Guangxi Zhuang Autonomous Region Applicant before: Guangxi Yingkang Pharmaceutical Co., Ltd. |
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