CN108207613A - A kind of Cotton Breeding Methods of resisting verticillium - Google Patents
A kind of Cotton Breeding Methods of resisting verticillium Download PDFInfo
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- CN108207613A CN108207613A CN201711482292.7A CN201711482292A CN108207613A CN 108207613 A CN108207613 A CN 108207613A CN 201711482292 A CN201711482292 A CN 201711482292A CN 108207613 A CN108207613 A CN 108207613A
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- cotton
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- verticillium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
Abstract
The invention belongs to variety of crops breeding technique field, more particularly to a kind of Cotton Breeding Methods of resisting verticillium are removed the peel including seed selection, and mutagens impregnate germination；It chooses verticillium wilt to take place frequently field, the dry branches and fallen leaves and residuum for collecting verticillium wilt cotton are layed in field, obtain breeding nursery；In seedling replanting breeding nursery, add the random emasculation of ultra violet lamp plant or go female, using interspecific hybridization, therefrom filter out disease-resistant plant after plant maturation, obtain F1Generation；F1In generation, is directly seeded in breeding nursery, and interspecific hybridization obtains F2In generation, the later stage continues bagging selfing, until the cotton variety that best in quality, resisting verticillium character is stablized.The present invention cuts gined cotton flower seed using the immersion of dithyl sulfate mutagens, mutation breeding is carried out to cotton seeds, and add ultraviolet increasing mutagenesis dynamics, disease-resistant plant is filtered out by the highly concentrated breeding nursery of verticillium wilt pathogen, new verticillium wilt-resistant cotton kind is selected using hybridization, selfing purifying genetic make up.
Description
Technical field
The invention belongs to variety of crops breeding technique field, more particularly to a kind of Cotton Breeding Methods of resisting verticillium.
Background technology
Cotton is Malvaceae (Malvaceae) Gossypium (Gossypium), originates in subtropical zone, there is Asiatic cotton, cotton, land
Four Shoot apex of ground cotton and sea island cotton, the cotton cultivated in the world is mainly upland cotton and sea island cotton.The kind of China upland cotton
It plants area and accounts for more than the 98% of the national cotton field gross area, there is the plantation of sea island cotton in Some Regions, Xinjiang.China is divided into three big main products
Cotton region, including the Yangtze river basin, Yellow River basin and Northwest inland cotton region, nowadays cotton has become one of main economic crops in China.
Cotton verticillium wilt (Verticillium dahliae.Kleb.) is Ragnarok soil-borne vascular bundle disease, seriously
Influence cotton yield and quality, a variety of herbaceous crops, tree crop, flowers and woody ornamentais can be infected, as strawberry, kind
Eggplant, peppermint, Chinese olive tree, capsicum, rape, eggplant, potato, sunflower, chrysanthemum, rose, maple etc..Verticillium wilt is the most heavy of cotton
One of disease is wanted, the title for having " cancer " of cotton, nineteen thirty-five is passed to China.It is controlled from end of the eighties in last century droop
Afterwards, verticillium wilt rises to the first major disease of cotton.Since the 1990s, which has spread each main product cotton region in China, grave illness
The diseased plant rate in cotton field is often up to more than 80%, and production loss is serious.1993rd, verticillium wilt continuous large-area hair in 1995 and 1996
It is raw, wherein national cotton verticillium wilt disaster area in 1993, up to 40,000,000 mu (266.67 ten thousand hm2), loss gined cotton 2,000,000 carries on a shoulder pole (1
Hundred million kg), especially North china has many cotton fields the phenomenon that diseased plant fallen leaves in blocks are into light stalk occur, causes the significantly underproduction, with
Total crop failure is caused, the threat of verticillium wilt has become an outstanding problem for restricting Cotton Production.For this purpose, the present invention have studied it is a kind of anti-yellowing
The Cotton Breeding Methods for disease of withering.
Invention content
The present invention cuts gined cotton flower seed using the immersion of dithyl sulfate mutagens, and cotton seeds are carried out with mutation breeding, and
Add ultraviolet increasing mutagenesis dynamics, disease-resistant plant is filtered out by the highly concentrated breeding nursery of verticillium wilt pathogen, purified using hybridization, selfing
Genetic make up selects new verticillium wilt-resistant cotton kind.
The present invention provides a kind of Cotton Breeding Methods of resisting verticillium, include the following steps：
S1 selects complete, full cotton seeds, and seed embryo butt kind skin is cut by grain, be then laid in using sand as
In the seedlings nursing plate of bud bed, Ensure Liquid liquid is to seed is submerged, and every liter of nutrient solution is by the basic nutrient solution of 992ml and the sulfuric acid two of 8ml
Ethyl ester-phosphate buffer mixes, and is cultivated under natural lighting, 1-3 pieces spire to be grown, and it is spare to obtain seedling；
S2 chooses the field that verticillium wilt takes place frequently, and the dry branches and fallen leaves and residuum for collecting verticillium wilt cotton are layed in the field,
Thickness is 3-5cm, applies 10-15kg/ mus of glucose, natural degradation；
S3, coming year repetition S2 steps are primary, and field site preparation is loosened the soil, obtains breeding nursery；
S4, by breeding nursery in the seedling replanting to S3 in S1, line-spacing and the random disunity of spacing in the rows add 30W ultraviolet lamps to shine
Penetrate 30-45d, frequency is 3-5d/ times, each 3-6h, the random emasculation of plant or go it is female, using interspecific hybridization, after plant maturation from
In filter out disease-resistant plant, obtain F1Generation, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing；
S5, by F1In generation, is directly seeded in breeding nursery, adds 30W ultra violet lamp 30-45d, and frequency is 3-5d/ times, every time
3-6h, the random emasculation of plant or goes female, using interspecific hybridization, therefrom filters out disease-resistant plant after plant maturation, obtains F2Generation, dye are yellow
The harvesting of disease of withering plant is directly spilt in breeding nursery after crushing；
S6, by F2In generation, is directly seeded in breeding nursery, and bagging is isolated, using selfing, disease-resistant plant is filtered out after plant maturation
Strain, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing, and obtain F3Generation；
S7 repeats S6 steps, until obtaining the cotton variety that best in quality, resisting verticillium character is stablized.
Preferably, the formula of every liter of basic nutrient solution is in S1：Potassium nitrate 30g/L, calcium sulphate dihydrate 3g/L, seven water sulfuric acid
Magnesium 9g/L, ammonium nitrate 40g/L, potassium dihydrogen phosphate 5g/L, remaining is distilled water.
Preferably, it is 7 that dithyl sulfate-phosphate buffer, which is pH value, in S1, to the 1000ml phosphoric acid hydrogen two of 0.5mol/L
3ml dithyl sulfates are added in sodium solution, are made after mixing.
Preferably, in S3 in breeding nursery soil verticillium wilt pathogen viable count in 300/more than g.
Compared with prior art, beneficial effects of the present invention：
The present invention impregnates cotton seeds using dithyl sulfate mutagens, carries out mutation breeding to cotton seeds, cuts simultaneously
Radiculodium kind skin is removed, improves efficiency of inducing mutation, in breeding nursery plus ultra violet lamp can improve mutagenesis dynamics, expand range of variation,
Disease-resistant plant is filtered out by the highly concentrated breeding nursery of verticillium wilt pathogen；Line-spacing spacing in the rows is mixed and disorderly in incubation, leads between reduction plant
Wind, and each generation all will dye verticillium wilt plant crush after spill in breeding nursery, be conducive to improve resisting verticillium plant high pressure
Selection；New verticillium wilt-resistant cotton kind is selected using hybridization, selfing purifying genetic make up.
Specific embodiment
3 specific embodiments of the present invention are described in detail below, it is to be understood that protection scope of the present invention
It is not restricted by specific implementation, the experimental method used in embodiment is conventional method unless otherwise specified, real
Material used in example is applied, is commercially available.
Embodiment 1
S1, selects hundred complete, full cotton BM04113 cotton seeds, cuts seed embryo butt kind skin by grain, does not scratch embryo
Then bud is laid in the seedlings nursing plate using sand as bud bed, Ensure Liquid liquid to submerging seed, every liter of nutrient solution by 992ml base
Dithyl sulfate-phosphate buffer of plinth nutrient solution and 8ml mix, and the formula of every liter of basic nutrient solution is：Potassium nitrate
30g/L, calcium sulphate dihydrate 3g/L, epsom salt 9g/L, ammonium nitrate 40g/L, potassium dihydrogen phosphate 5g/L, remaining is distilled water,
Dithyl sulfate-phosphate buffer is that pH value is 7,3ml sulfuric acid is added in into the 1000ml disodium phosphate solns of 0.5mol/L
Diethylester is made after mixing, is cultivated under natural lighting, 1-3 pieces spire to be grown, and it is spare to obtain seedling；
S2, chooses the field that takes place frequently of moistening verticillium wilt of water content 80%, the dry branches and fallen leaves of collection verticillium wilt cotton and residual
Body is layed in the field, thickness 3cm, applies 10kg/ mus of glucose, natural degradation；
S3, repeats that S2 steps are primary, and verticillium wilt pathogen viable count is in 300/more than g in soil, by field site preparation pine the coming year
Soil obtains breeding nursery；
S4, by breeding nursery in the seedling replanting to S3 in S1, line-spacing and the random disunity of spacing in the rows add 30W ultraviolet lamps to shine
Penetrate 30d, frequency is 3d/ times, each 3h, the random emasculation of plant or goes female, using interspecific hybridization, is therefrom filtered out after plant maturation
Disease-resistant plant obtains F1Generation, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing；
S5, by F1In generation, is directly seeded in breeding nursery, adds 30W ultra violet lamp 30d, and frequency is 3d/ times, each 3h, is planted
The random emasculation of strain is gone female, using interspecific hybridization, is therefrom filtered out disease-resistant plant after plant maturation, is obtained F2Generation, dye verticillium wilt are planted
Strain harvesting is directly spilt in breeding nursery after crushing；
S6, by F2In generation, is directly seeded in breeding nursery, and bagging is isolated, using selfing, disease-resistant plant is filtered out after plant maturation
Strain, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing, and obtain F3In generation, repeats the above steps, F10It is excellent that in generation, obtains quality
The cotton variety that good, resisting verticillium character is stablized.
The new cotton variety that best in quality, resisting verticillium character obtained above is stablized is planted in common cotton field
In, cultivation management is carried out using existing conventional cultivation mode, statistics shows that the plant of resisting verticillium accounts for 99%, gined cotton production 80kg/
Mu.
Embodiment 2
S1, selects hundred complete, full cotton BMY1-1 cotton seeds, cuts seed embryo butt kind skin by grain, then tiles
In in the seedlings nursing plate using sand as bud bed, Ensure Liquid liquid to submerging seed, every liter of nutrient solution by 992ml basic nutrient solution and
Dithyl sulfate-phosphate buffer of 8ml mixes, and the formula of every liter of basic nutrient solution is：Potassium nitrate 30g/L, two water sulphur
Sour calcium 3g/L, epsom salt 9g/L, ammonium nitrate 40g/L, potassium dihydrogen phosphate 5g/L, remaining is distilled water, dithyl sulfate-phosphorus
Acid buffer is that pH value is 7,3ml dithyl sulfates are added in into the 1000ml disodium phosphate solns of 0.5mol/L, after mixing
It is made, is cultivated under natural lighting, 1-3 pieces spire to be grown, it is spare to obtain seedling；
S2, chooses the field that takes place frequently of moistening verticillium wilt of water content 75%, the dry branches and fallen leaves of collection verticillium wilt cotton and residual
Body is layed in the field, thickness 5cm, applies 15kg/ mus of glucose, natural degradation；
S3, repeats that S2 steps are primary, and verticillium wilt pathogen viable count is in 300/more than g in soil, by field site preparation pine the coming year
Soil obtains breeding nursery；
S4, by breeding nursery in the seedling replanting to S3 in S1, line-spacing and the random disunity of spacing in the rows add 30W ultraviolet lamps to shine
Penetrate 45d, frequency is 5d/ times, each 6h, the random emasculation of plant or goes female, using interspecific hybridization, is therefrom filtered out after plant maturation
Disease-resistant plant obtains F1Generation, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing；
S5, by F1In generation, is directly seeded in breeding nursery, adds 30W ultra violet lamp 45d, and frequency is 5d/ times, each 6h, is planted
The random emasculation of strain is gone female, using interspecific hybridization, is therefrom filtered out disease-resistant plant after plant maturation, is obtained F2Generation, dye verticillium wilt are planted
Strain harvesting is directly spilt in breeding nursery after crushing；
S6, by F2In generation, is directly seeded in breeding nursery, and bagging is isolated, using selfing, disease-resistant plant is filtered out after plant maturation
Strain, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing, and obtain F3In generation, repeats the above steps, F13It is excellent that in generation, obtains quality
The cotton variety that good, resisting verticillium character is stablized.
The new cotton variety that best in quality, resisting verticillium character obtained above is stablized is planted in common cotton field
In, cultivation management is carried out using existing conventional cultivation mode, statistics shows that the plant of resisting verticillium accounts for 99.5%, gined cotton production
79.3kg/ mus.
Embodiment 3
A kind of Cotton Breeding Methods of resisting verticillium, include the following steps：
S1 selects the cotton seeds of complete, full Ji 668, and seed embryo butt kind skin is cut by grain, be then laid in
Sand in the seedlings nursing plate of bud bed, Ensure Liquid liquid to submerging seed, every liter of nutrient solution by 992ml basic nutrient solution and 8ml
Dithyl sulfate-phosphate buffer mixes, and the formula of every liter of basic nutrient solution is：Potassium nitrate 30g/L, calcium sulphate dihydrate
3g/L, epsom salt 9g/L, ammonium nitrate 40g/L, potassium dihydrogen phosphate 5g/L, remaining is distilled water, and dithyl sulfate-phosphoric acid delays
Fliud flushing is that pH value is 7,3ml dithyl sulfates are added in into the 1000ml disodium phosphate solns of 0.5mol/L, is made after mixing,
It is cultivated under natural lighting, 1-3 pieces spire to be grown, it is spare to obtain seedling；
S2, chooses the field that takes place frequently of moistening verticillium wilt of water content 82%, the dry branches and fallen leaves of collection verticillium wilt cotton and residual
Body is layed in the field, thickness 4cm, applies 12kg/ mus of glucose, natural degradation；
S3, repeats that S2 steps are primary, and verticillium wilt pathogen viable count is in 300/more than g in soil, by field site preparation pine the coming year
Soil obtains breeding nursery；
S4, by breeding nursery in the seedling replanting to S3 in S1, line-spacing and the random disunity of spacing in the rows add 30W ultraviolet lamps to shine
Penetrate 40d, frequency is 4d/ times, each 4h, the random emasculation of plant or goes female, using interspecific hybridization, is therefrom filtered out after plant maturation
Disease-resistant plant obtains F1Generation, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing；
S5, by F1In generation, is directly seeded in breeding nursery, adds ultra violet lamp 40d, and frequency is 4d/ times, each 4h, plant with
Machine emasculation is gone female, using interspecific hybridization, is therefrom filtered out disease-resistant plant after plant maturation, is obtained F2Generation, dye verticillium wilt plant are received
It is directly spilt in breeding nursery after cutting crushing；
S6, by F2In generation, is directly seeded in breeding nursery, and bagging is isolated, using selfing, disease-resistant plant is filtered out after plant maturation
Strain, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing, and obtain F3In generation, repeats the above steps, F16It is excellent that in generation, obtains quality
The cotton variety that good, resisting verticillium character is stablized.
The new cotton variety that best in quality, resisting verticillium character obtained above is stablized is planted in common cotton field
In, cultivation management is carried out using existing conventional cultivation mode, statistics shows that the plant of resisting verticillium accounts for 99.7%, gined cotton production
78kg/ mus.
The new breeding method of verticillium wilt-resistant cotton for understanding the present invention by above-described embodiment 1-3 is suitable for multiple Gossypium kinds,
And the new varieties of selection and breeding are best in quality, disease resistance is good, character is stablized, and has the economic value of Promotion practice.
It should be noted that the step method used in claims of the present invention is same as the previously described embodiments, in order to anti-
Only repeat, description of the invention preferred embodiment, but those skilled in the art once know it is basic creative general
It reads, then other change and modification can be made to these embodiments.So appended claims are intended to be construed to include preferably in fact
It applies example and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (4)
1. a kind of Cotton Breeding Methods of resisting verticillium, which is characterized in that include the following steps：
S1 selects complete, full cotton seeds, cuts seed embryo butt kind skin by grain, is then laid in using sand as bud bed
Seedlings nursing plate in, Ensure Liquid liquid to submerging seed, every liter of nutrient solution by 992ml basic nutrient solution and 8ml dithyl sulfate-
Phosphate buffer mixes, and is cultivated under natural lighting, 1-3 pieces spire to be grown, and it is spare to obtain seedling；
S2 chooses the field that verticillium wilt takes place frequently, and the dry branches and fallen leaves and residuum for collecting verticillium wilt cotton are layed in the field, thickness
For 3-5cm, 10-15kg/ mus of glucose, natural degradation are applied；
S3, coming year repetition S2 steps are primary, and field site preparation is loosened the soil, obtains breeding nursery；
S4, by breeding nursery in the seedling replanting to S3 in S1, line-spacing and the random disunity of spacing in the rows add 30W ultra violet lamps 30-
45d, frequency are 3-5d/ times, each 3-6h, the random emasculation of plant or go female, using interspecific hybridization, are therefrom screened after plant maturation
Go out disease-resistant plant, obtain F1Generation, the plant harvesting of dye verticillium wilt are directly spilt in breeding nursery after crushing；
S5, by F1In generation, is directly seeded in breeding nursery, adds 30W ultra violet lamp 30-45d, and frequency is 3-5d/ times, each 3-6h,
The random emasculation of plant is gone female, using interspecific hybridization, is therefrom filtered out disease-resistant plant after plant maturation, is obtained F2In generation, contaminates verticillium wilt
Plant harvesting is directly spilt in breeding nursery after crushing；
S6, by F2In generation, is directly seeded in breeding nursery, and bagging is isolated, using selfing, disease-resistant plant is filtered out after plant maturation, is contaminated
The harvesting of verticillium wilt plant is directly spilt in breeding nursery after crushing, and obtains F3Generation；
S7 repeats S6 steps, until obtaining the cotton variety that best in quality, resisting verticillium character is stablized.
2. the Cotton Breeding Methods of resisting verticillium as described in claim 1, which is characterized in that every liter of basic nutrient solution in S1
It is formulated and is：Potassium nitrate 30g/L, calcium sulphate dihydrate 3g/L, epsom salt 9g/L, ammonium nitrate 40g/L, potassium dihydrogen phosphate 5g/L,
Remaining is distilled water.
3. the Cotton Breeding Methods of resisting verticillium as described in claim 1, which is characterized in that dithyl sulfate-phosphoric acid in S1
Buffer solution is that pH value is 7, and 3ml dithyl sulfates are added in into the 1000ml disodium phosphate solns of 0.5mol/L, are made after mixing
.
4. the Cotton Breeding Methods of resisting verticillium as described in claim 1, which is characterized in that Huang withers in breeding nursery soil in S3
Germ viable count is in 300/more than g.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169127A (en) * | 2018-09-27 | 2019-01-11 | 湖北省农业科学院经济作物研究所 | A kind of Cotton Breeding Methods of resisting verticillium |
| CN109644868A (en) * | 2018-12-25 | 2019-04-19 | 上海太和水环境科技发展股份有限公司 | A kind of selection of eel grass |
| CN111226786A (en) * | 2020-02-06 | 2020-06-05 | 内蒙古自治区农牧业科学院 | Method for screening cotton varieties suitable for mechanical harvesting |
| CN112400646A (en) * | 2020-11-23 | 2021-02-26 | 湖北省农业科学院经济作物研究所 | Anti-verticillium wilt cotton breeding method |
| CN112400645A (en) * | 2020-11-16 | 2021-02-26 | 安徽省农业科学院棉花研究所 | Method for cultivating new high-temperature-resistant cotton variety |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169127A (en) * | 2018-09-27 | 2019-01-11 | 湖北省农业科学院经济作物研究所 | A kind of Cotton Breeding Methods of resisting verticillium |
| CN109644868A (en) * | 2018-12-25 | 2019-04-19 | 上海太和水环境科技发展股份有限公司 | A kind of selection of eel grass |
| CN111226786A (en) * | 2020-02-06 | 2020-06-05 | 内蒙古自治区农牧业科学院 | Method for screening cotton varieties suitable for mechanical harvesting |
| CN112400645A (en) * | 2020-11-16 | 2021-02-26 | 安徽省农业科学院棉花研究所 | Method for cultivating new high-temperature-resistant cotton variety |
| CN112400645B (en) * | 2020-11-16 | 2024-03-29 | 安徽省农业科学院棉花研究所 | Cultivation method of high-temperature-resistant cotton new variety |
| CN112400646A (en) * | 2020-11-23 | 2021-02-26 | 湖北省农业科学院经济作物研究所 | Anti-verticillium wilt cotton breeding method |
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Inventor after: Wang Qinglian Inventor after: Zhang Zhiyong Inventor after: Qin Tengfei Inventor after: Xue Huiyun Inventor after: Zhang Xiaohong Inventor after: Tan Yangguang Inventor after: Han Zhenfu Inventor after: Dong Tao Inventor after: Wei Chunyan Inventor after: Hu Genhai Inventor after: Zhang Jinbao Inventor after: Sun Runrun Inventor after: Dong Na Inventor after: Fu Yuanzhi Inventor after: Wang Yuanyuan Inventor after: Li Chengqi Inventor after: Zhang Xin Inventor before: Wang Qinglian Inventor before: Zhang Zhiyong Inventor before: Qin Tengfei Inventor before: Xue Huiyun Inventor before: Zhang Xiaohong Inventor before: Tan Yangguang Inventor before: Han Zhenfu Inventor before: Dong Tao Inventor before: Wei Chunyan Inventor before: Hu Genhai Inventor before: Zhang Jinbao Inventor before: Sun Runrun Inventor before: Dong Na Inventor before: Fu Yuanzhi Inventor before: Wang Yuanyuan Inventor before: Li Chengqi Inventor before: Zhang Xin |
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Application publication date: 20180629 |
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| RJ01 | Rejection of invention patent application after publication |