CN108203710B - 一种利用纯秸秆固体补料诱导里氏木霉产纤维素酶的方法及其使用的补料器 - Google Patents
一种利用纯秸秆固体补料诱导里氏木霉产纤维素酶的方法及其使用的补料器 Download PDFInfo
- Publication number
- CN108203710B CN108203710B CN201810076624.XA CN201810076624A CN108203710B CN 108203710 B CN108203710 B CN 108203710B CN 201810076624 A CN201810076624 A CN 201810076624A CN 108203710 B CN108203710 B CN 108203710B
- Authority
- CN
- China
- Prior art keywords
- enzyme
- feeding
- frequency converter
- fermentation tank
- dissolved oxygen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000463 material Substances 0.000 title claims abstract description 51
- 239000010902 straw Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 27
- 108010059892 Cellulase Proteins 0.000 title claims abstract description 21
- 229940106157 cellulase Drugs 0.000 title claims abstract description 20
- 239000011343 solid material Substances 0.000 title claims abstract description 16
- 241000499912 Trichoderma reesei Species 0.000 title claims abstract description 15
- 230000001939 inductive effect Effects 0.000 title claims abstract description 10
- 230000001502 supplementing effect Effects 0.000 title claims description 27
- 108090000790 Enzymes Proteins 0.000 claims abstract description 71
- 102000004190 Enzymes Human genes 0.000 claims abstract description 71
- 229940088598 enzyme Drugs 0.000 claims abstract description 71
- 238000000855 fermentation Methods 0.000 claims abstract description 64
- 230000004151 fermentation Effects 0.000 claims abstract description 63
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 62
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 62
- 239000001301 oxygen Substances 0.000 claims abstract description 62
- 238000004519 manufacturing process Methods 0.000 claims abstract description 41
- 239000013589 supplement Substances 0.000 claims abstract description 24
- 239000007787 solid Substances 0.000 claims abstract description 20
- 238000011081 inoculation Methods 0.000 claims abstract description 8
- 239000006052 feed supplement Substances 0.000 claims abstract description 7
- 230000008569 process Effects 0.000 claims abstract description 7
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 4
- 230000001105 regulatory effect Effects 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 21
- 150000003839 salts Chemical class 0.000 claims description 19
- 240000008042 Zea mays Species 0.000 claims description 16
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 16
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 16
- 235000005822 corn Nutrition 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 7
- 229910052564 epsomite Inorganic materials 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- 239000004202 carbamide Substances 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 238000004880 explosion Methods 0.000 claims description 4
- 238000010899 nucleation Methods 0.000 claims description 4
- 239000000523 sample Substances 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- 239000003242 anti bacterial agent Substances 0.000 claims description 3
- 229940088710 antibiotic agent Drugs 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 238000007747 plating Methods 0.000 claims description 3
- 230000001276 controlling effect Effects 0.000 claims description 2
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims 1
- 229910052603 melanterite Inorganic materials 0.000 claims 1
- 235000013619 trace mineral Nutrition 0.000 claims 1
- 239000011573 trace mineral Substances 0.000 claims 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims 1
- 229910000368 zinc sulfate Inorganic materials 0.000 claims 1
- 239000011686 zinc sulphate Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 230000008859 change Effects 0.000 abstract description 6
- 239000002904 solvent Substances 0.000 abstract description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 13
- 229910052799 carbon Inorganic materials 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 8
- 239000001913 cellulose Substances 0.000 description 7
- 235000010980 cellulose Nutrition 0.000 description 7
- 229920002678 cellulose Polymers 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 3
- 230000001976 improved effect Effects 0.000 description 3
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 3
- 239000008108 microcrystalline cellulose Substances 0.000 description 3
- 229940016286 microcrystalline cellulose Drugs 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 230000000630 rising effect Effects 0.000 description 3
- 229920002261 Corn starch Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000452385 Trichoderma reesei RUT C-30 Species 0.000 description 2
- 239000008120 corn starch Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 230000036284 oxygen consumption Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000008093 supporting effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- HIWPGCMGAMJNRG-ACCAVRKYSA-N Sophorose Natural products O([C@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HIWPGCMGAMJNRG-ACCAVRKYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- HIWPGCMGAMJNRG-UHFFFAOYSA-N beta-sophorose Natural products OC1C(O)C(CO)OC(O)C1OC1C(O)C(O)C(O)C(CO)O1 HIWPGCMGAMJNRG-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 101150052795 cbh-1 gene Proteins 0.000 description 1
- 101150114858 cbh2 gene Proteins 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 101150066032 egl-1 gene Proteins 0.000 description 1
- 101150003727 egl2 gene Proteins 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000012526 feed medium Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- PZDOWFGHCNHPQD-VNNZMYODSA-N sophorose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](C=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PZDOWFGHCNHPQD-VNNZMYODSA-N 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/16—Screw conveyor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Computer Hardware Design (AREA)
- Medicinal Chemistry (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
起始补料时间点 | 补料时长 | 固体终浓度 | 纤维素酶活力 |
D点 | 72h | 10% | 29IU/mL |
E点 | 72h | 10% | 20IU/mL |
F点 | 72h | 10% | 40IU/mL |
G点 | 72h | 10% | 32IU/mL |
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810076624.XA CN108203710B (zh) | 2018-01-26 | 2018-01-26 | 一种利用纯秸秆固体补料诱导里氏木霉产纤维素酶的方法及其使用的补料器 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810076624.XA CN108203710B (zh) | 2018-01-26 | 2018-01-26 | 一种利用纯秸秆固体补料诱导里氏木霉产纤维素酶的方法及其使用的补料器 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108203710A CN108203710A (zh) | 2018-06-26 |
CN108203710B true CN108203710B (zh) | 2021-02-23 |
Family
ID=62605777
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810076624.XA Active CN108203710B (zh) | 2018-01-26 | 2018-01-26 | 一种利用纯秸秆固体补料诱导里氏木霉产纤维素酶的方法及其使用的补料器 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108203710B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111944788B (zh) * | 2020-08-17 | 2021-04-23 | 上海汉禾生物新材料科技有限公司 | 一种诱导里氏木霉产纤维素酶的方法 |
CN112175842B (zh) * | 2020-11-13 | 2022-12-27 | 上海汉禾生物新材料科技有限公司 | 一种里氏木霉发酵高产纤维素酶的方法 |
CN112795491B (zh) * | 2021-01-29 | 2023-03-17 | 武汉新华扬生物股份有限公司 | 一种里氏木霉生产高活性酸性纤维素酶的发酵方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101351543A (zh) * | 2005-11-07 | 2009-01-21 | 维文蒂阿生物技术股份有限公司 | 可控发酵的方法和步骤 |
CN201984344U (zh) * | 2011-02-23 | 2011-09-21 | 陈晨 | 一种微生物发酵设备监控系统 |
CN103008333A (zh) * | 2012-12-31 | 2013-04-03 | 江苏大学 | 一种秸秆碎末废弃物资源化利用成套装备及基质化方法 |
CN103509883A (zh) * | 2012-06-23 | 2014-01-15 | 威志德(北京)技术开发有限公司 | 新颖的适用于多种微生物发酵的补料调控工艺 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN201506796U (zh) * | 2009-07-23 | 2010-06-16 | 上海海洋大学 | 微生物培养用智能补料装置 |
CN201506794U (zh) * | 2009-07-23 | 2010-06-16 | 上海海洋大学 | 一种微生物培养用反馈补料控制装置 |
CN102229920B (zh) * | 2011-07-21 | 2013-03-13 | 天津工业生物技术研究所 | 一种提高里氏木霉纤维素酶液体深层发酵水平的方法 |
CN103045566A (zh) * | 2012-12-14 | 2013-04-17 | 河南天冠纤维乙醇有限公司 | 一种利用底物诱导调控纤维素酶生产的方法 |
CN103103220A (zh) * | 2013-01-18 | 2013-05-15 | 首都师范大学 | 以汽爆法处理的木质纤维素为原料的同步糖化和乙醇发酵法 |
TWI530562B (zh) * | 2013-10-31 | 2016-04-21 | 行政院原子能委員會核能研究所 | 一種提高纖維分解酵素活性之生產方法 |
CN103740680B (zh) * | 2013-12-23 | 2016-04-20 | 湖南鸿鹰生物科技有限公司 | 里氏木霉发酵生产纤维素酶的方法及其菌株应用 |
-
2018
- 2018-01-26 CN CN201810076624.XA patent/CN108203710B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101351543A (zh) * | 2005-11-07 | 2009-01-21 | 维文蒂阿生物技术股份有限公司 | 可控发酵的方法和步骤 |
CN201984344U (zh) * | 2011-02-23 | 2011-09-21 | 陈晨 | 一种微生物发酵设备监控系统 |
CN103509883A (zh) * | 2012-06-23 | 2014-01-15 | 威志德(北京)技术开发有限公司 | 新颖的适用于多种微生物发酵的补料调控工艺 |
CN103008333A (zh) * | 2012-12-31 | 2013-04-03 | 江苏大学 | 一种秸秆碎末废弃物资源化利用成套装备及基质化方法 |
Also Published As
Publication number | Publication date |
---|---|
CN108203710A (zh) | 2018-06-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108203710B (zh) | 一种利用纯秸秆固体补料诱导里氏木霉产纤维素酶的方法及其使用的补料器 | |
CN103243023B (zh) | 秸秆同步酶解发酵燃料乙醇循环式反应器及其反应方法 | |
CN112795491B (zh) | 一种里氏木霉生产高活性酸性纤维素酶的发酵方法 | |
CN101575617A (zh) | 一种富铬酵母培养物及其发酵工艺 | |
CN104726510B (zh) | 一种发酵制备赖氨酸的方法 | |
CN105543314A (zh) | 一种应用发酵与泡沫分离耦合生产多粘菌素e的方法 | |
CN107760623B (zh) | 一株产中性生淀粉酶的阿氏芽孢杆菌 | |
CN103882081B (zh) | 一种连续流加补料分批发酵提高杆菌肽效价的方法 | |
CN105925551A (zh) | 基于葡萄糖转苷反应制备混合物高效生产纤维素酶的方法 | |
CN103695315B (zh) | 一种用微生物发酵生产甲壳低聚糖的方法 | |
CN104357428A (zh) | 一种木聚糖酶的液态深层发酵方法 | |
CN102559782A (zh) | 利用酪丁酸梭菌发酵甘蔗渣水解液生产丁酸的工艺 | |
CN111944788B (zh) | 一种诱导里氏木霉产纤维素酶的方法 | |
CN104561140B (zh) | 一种发酵制备柠檬酸的方法 | |
CN117604057A (zh) | 一种提高透明质酸生产效率的方法 | |
CN105176853A (zh) | 一株同时生产甲醇蛋白和木聚糖酶的毕赤酵母及其应用 | |
CN100527979C (zh) | 一种微生物降解羽毛的方法 | |
CN111394397A (zh) | 一种利用餐厨垃圾发酵生产己酸的方法 | |
CN106035985A (zh) | 一种混菌液态发酵黄酒加工废弃物生产单细胞蛋白的方法 | |
CN103146769A (zh) | 一种发酵制备柠檬酸的方法 | |
CN106086093A (zh) | 乳酸发酵菌体渣预处理方法及循环发酵生产乳酸的方法 | |
CN102251002A (zh) | 固态发酵矛尾刺虾虎鱼肌肉蛋白制备抗氧化多肽的方法 | |
CN102051386A (zh) | 间歇式回流细胞高生产率发酵生产有机酸的方法 | |
CN102766656A (zh) | 一种利用甘蔗渣廉价制备微生物絮凝剂的方法 | |
CN103497977A (zh) | 一种发酵制备柠檬酸的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210220 Address after: Room 202, building 21, 2388 Chenhang Road, Minhang District, Shanghai 201100 Patentee after: SHANGHAI HANHE BIOLOGICAL NEW MATERIAL TECHNOLOGY Co.,Ltd. Address before: 226200 No.3 Jiangfeng Road, Binhai Industrial Park, Qidong City, Nantong City, Jiangsu Province Patentee before: JIANGSU DIYIN BIOTECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231029 Address after: Room 406, 4th Floor, Youth Entrepreneurship Park Complex Building, No. 399 Jiayi Road, Lingxi Town, Cangnan County, Wenzhou City, Zhejiang Province, 325899 Patentee after: Zhejiang Canghai Heneng New Material Technology Co.,Ltd. Address before: Room 202, building 21, 2388 Chenhang Road, Minhang District, Shanghai 201100 Patentee before: SHANGHAI HANHE BIOLOGICAL NEW MATERIAL TECHNOLOGY Co.,Ltd. |