CN108112364A - A kind of screening technique of resistance to female ground cover plant - Google Patents
A kind of screening technique of resistance to female ground cover plant Download PDFInfo
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- CN108112364A CN108112364A CN201711479886.2A CN201711479886A CN108112364A CN 108112364 A CN108112364 A CN 108112364A CN 201711479886 A CN201711479886 A CN 201711479886A CN 108112364 A CN108112364 A CN 108112364A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a kind of screening techniques of resistance to female ground cover plant, including test garden select, test block division, vegetable material preparation, planting, shading treatment, management and shade tolerant merit rating.The method of the present invention is used as screening experiment material by the consistent cuttage seeding of cutting propagation a batch growing way, reduces experimental error caused by the difference existing between vegetable material individual, reduces the influence of the other factors in addition to illumination factor.Under the natural shading environment of this experiment simulation at 4 kinds, shade tolerance measure has been carried out to 30 kinds of Wild groundcover plants using simplicity, science, accurate method, it it is 4 kinds by plant shade tolerant Type division, and 17 kinds of stronger plants of shade tolerance have been screened, scope of experiment is big, and data are reliable, improve screening efficiency, it is a kind of resistance to female ground cover plant screening technique that high practical value, is conducive to understand the shade tolerant type of plant, so as to introduces a fine variety, cultivates extensively and applying beneficial to ground cover plant.
Description
Technical field
The present invention relates to foliage filter screening technical fields, and in particular to a kind of screening technique of resistance to female ground cover plant.
Background technology
The construction of urban infrastructure causes city grey building to continue to increase, and greenery construction is constantly being reduced, and is
Balance city grey construction and the development of greenery construction, vertical greening come into being, and add a large amount of greenery patchess.However, it is building
If in the process, we not only need to consider the greenery construction of vertical direction, the greenery construction in horizontal direction also seems particularly heavy
It wants, the greening of the raw environment of the shade to be formed, the life under the raw environment of shade of general plant variety is especially blocked by a large amount of high-lager buildings
Length is in poor shape or is not easy to survive.Ground cover plant refers to that strain clump is close, short or overgrow, and breeding time is in outdoor cropping, covering power
By force, the rapid a kind of plant of growth, can obtain good appreciation effect in a short time, it may have resistance to trimming, covering power be strong,
The features such as extensive management, has been more and more widely used in gardens, and demand also continues to increase.Therefore, screening has resistance to
The ground cover plant of cloudy ability has extremely important meaning to urban afforestation, beautification.
Existing plant shade tolerance correlative study is that vegetable material to be measured is directly planted in the shading of manual simulation mostly
In environment, analyze its variation of physiological characteristic under the conditions of different shadings and carry out shade tolerance evaluation or shade tolerant capability analysis, not yet
There is the report of more comprehensive resistance to female ground cover plant screening technique, in order to reduce experimental error, improve screening efficiency, it is necessary to pay close attention to
The influence of place election, vegetable material preparation etc..Therefore, it is necessary to a kind of highly effective and comprehensive resistance to female ground cover plants
Screening technique, convenient for from now on to introduce a fine variety material carry out shade tolerant Journal of Sex Research, screen excellent resistance to female plant.
The content of the invention
It is an object of the invention to provide a kind of screening technique of resistance to female ground cover plant, to solve in above-mentioned background technology
The problem of proposition.
To achieve these goals, the present invention provides following technical solution：A kind of screening technique of resistance to female ground cover plant,
Comprise the following steps：
（1）Select to test garden：It should select to have good geographical position, physical features is smooth, has abundant water resources, and irrigation condition is good, well-ventilated,
With doing garden, build greenhouse in plot away from pollution sources；
（2）Test block division：Each growing area block is divided according to plant material grown feature and experimental program, including maternal original seed
Area, maternal breeding area, screening test area, and turned over, raked the soil level, ridging and bedding；
（3）Vegetable material prepares：To avoid the difference between plant individual, acquisition length is consistent from female parent, thickness is consistent, raw
The consistent cuttings of long situation carries out cuttage, breeds the consistent experiment material of a collection of growing way；
（4）Planting：After 30 ~ 45 d of cuttage, the indefinite root system of base portion in great numbers, cauline leaf well-grown, same breed growing way are selected
Consistent vegetable material is planted in screening test area；
（5）Shading treatment：Different shading treatments is realized by building shading net outside greenhouse；
（6）Management：Temperature, humidity, water-fertilizer nutrient are consistent in Control experiment area, other external factor are avoided to cause experiment larger
It influences, it is 15~28 DEG C to control temperature range, humidity range 60% ~ 80%, and liquid fertilizer sprayed once, sprays weekly 75,%00 bacterium weekly
1000 times of liquid of clear 1500 times of liquid of wettable powder or 80% carbendazol wettable powder fungicide are once；
（7）Shade tolerant merit rating：After shading treatment 4 months, the relevant form index and physical signs of each vegetable material are measured,
The shade tolerant ability of comprehensive analysis and judgement plant filters out the strong plant variety of shade tolerance.
Preferably, step（3）Described in cuttings be 6 ~ 10 cm being cut into from annotinous branch brachyplast item, it is each to insert
At least 1 section on fringe, clears up morphology lower leaflet, retains 2-3 fresh blades above morphology, with 80% Mancozeb
800 times of 1 ~ 2 min of liquid disinfectant of wettable powder are then immersed in 1 ~ 2 h in No. 1 solution of ABT root-inducing powders of 100 mg/kg, cuttage
It is vermiculite in volume ratio：Perlite：Nutrition Soil=1:1:In 2 cultivation matrix, cuttings embedded depth is spike length 1/3 ~ 1/2.
Preferably, step（4）Middle screening test area planting depth is in 15 ~ 20 cm, and plantation spacing is in 40 ~ 50cm.
Preferably, step（5）Described in different shading treatments be that different degrees of shading net is built outside greenhouse, realize and hide
Cloudy rate is 0%, 30%, 50% and 80%.
Preferably, step（7）Described in morphological index include plant height and leaf area, physical signs include chlorophyll content and
Chlorophyll fluorescence performance index by SPSS data analyses, compares same plant biological indicator under different shading treatments
Under situation of change and different shading treatment between different plants same biological indicator variation tendency.
Compared with prior art, the advantage of the invention is that：Present invention firstly discloses a kind of more comprehensively resistance to female
By the screening technique of plant, there is provided maternal original seed farm, maternal propagation nursery and screening test gardens, pass through cutting propagation a batch growing way
Consistent cuttage seeding reduces experimental error caused by the difference existing between vegetable material individual as screening experiment material,
Reduce the influence of the other factors in addition to illumination factor.This experiment simulation it is natural shading environment under, using simplicity, science,
Accurate method has carried out shade tolerance measure to 30 kinds of Wild groundcover plants, is 4 kinds by plant shade tolerant Type division, and screens
17 kinds of stronger plants of shade tolerance, scope of experiment is big, and data are reliable, improves screening efficiency, is that a kind of that high practical value is resistance to
Female ground cover plant screening technique is conducive to understand the shade tolerant type of plant, so as to the extensive cultivation beneficial to ground cover plant and answers
With.
Specific embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
Embodiment 1
A kind of screening technique of resistance to female ground cover plant, process are to build reality in Hangzhou Lv Feng gardens group nursery stock displaying base
Greenhouse is tested, 30 kinds of wild wet-proof plants plants are selected in April, 2016, and specific screening technique is as screening material：
（1）Select to test garden：Hangzhou Lv Feng gardens group nursery stock is selected to show that physical features is smooth in base, irrigation condition is good,
Greenhouse is built with doing garden in well-ventilated, the plot of in uniform light；
（2）Test block division：Experiment block is divided into maternal original seed area, maternal breeding area, screening test area, builds 5
Small-sized greenhouse, maternal original seed area and maternal breeding area are located at No. 1 greenhouse, build respectively No. 2, No. 3, No. 4, No. 5 greenhouses are as sieve
Trial zone is selected, carries out 4 kinds of different shading treatments.It turned over, raked the soil level, ridging and bedding；
（3）Vegetable material prepares：To avoid the difference between plant individual, on the female parent consistent from growing way acquisition length it is consistent,
The cuttings that thickness is consistent, upgrowth situation is consistent carries out cuttage, breeds the consistent experiment material of a collection of growing way, wherein cuttings is from one
The brachyplast item for 6 ~ 10 cm being cut on year raw branch, at least 1 section in each cuttings, removes morphology lower leaflet, retains
2-3 fresh blades, with 80% mancozeb wettable powder, 800 times of 1 ~ 2 min of liquid disinfectant, are then immersed in 100 above morphology
In No. 1 solution of ABT root-inducing powders of mg/kg 1 ~ 2 it is small when, cuttage volume ratio be vermiculite：Perlite：Nutrition Soil=1:1:2 cultivation
In matrix, cuttings embedded depth is spike length 1/3 ~ 1/2, is watered sooner or later daily, and cultivation matrix is kept to be in moisture state, is sprayed weekly
Applying 75% Bravo wettable powder, 1500 times of liquid prevents the generation of pest and disease damage, if there is serious diseased plant occurs in cuttings and rots
Strain, timely clear up, prevent the sprawling of disease；
（4）Planting：After 30 ~ 45 d of cuttage, the indefinite root system of base portion in great numbers, cauline leaf well-grown, same breed growing way are selected
Consistent vegetable material is planted in respectively in each greenhouse in screening test area, planting depth in 15 ~ 20 cm, plantation spacing 40 ~
50cm；
（5）Shading treatment：CK is realized by covering different degrees of shading net outside greenhouse（Shading rate about 0%）、S1（Shading rate
About 30%）、S2（Shading rate about 50%）And S3（Shading rate about 80%）4 kinds of different shading treatments；
（6）Management：Temperature, humidity, water-fertilizer nutrient are consistent in Control experiment area, other external factor are avoided to cause experiment larger
It influences, it is 15~28 DEG C to control temperature range, humidity range 60% ~ 80%, and liquid fertilizer sprayed once, sprays weekly 75,%00 bacterium weekly
1000 times of liquid of clear 1500 times of liquid of wettable powder or 80% carbendazol wettable powder fungicide are once；
（7）Shade tolerant merit rating：After shading treatment 4 months, the relevant form index and physical signs of each vegetable material are measured,
The shade tolerant ability of comprehensive analysis and judgement plant filters out the strong plant variety of shade tolerance.Morphological index includes plant height and leaf area,
Physical signs includes chlorophyll content and chlorophyll fluorescence performance index.The plant height of plant is measured April and September part, is calculated
Each plant relative growth yield.After shading treatment 3 months, the mature leaf under identical station acquisition difference shading treatment, each
Each processing of vegetable material chooses 5, and leaf area is measured using Portable scan type leaf area instrument.After shading treatment 4 months, take
The ripe functional leaf of test plant carries out the measure of chlorophyll content using portable Chlorophyll meter SPAD-502, often
The each kind of a processing chooses 3 leaves, and every measuring blade 6 times is finally averaged and records.After shading treatment 4 months,
At the selection cloudy day, use portable plant efficiency instrument（HandyPEA Hansatech, UK）Carry out chlorophyll fluorescence measure.Each material
Expect that 5 mature leafs are chosen in each processing, first by plant leaf blade 30 min of dark adaptation folder dark adaptation before measuring.It obtains initial glimmering
The parameters such as light (Fo), maximum fluorescence (Fm), variable fluorescence (Fv) calculate lightsystemⅡ maximal photochemistry efficiency (Fv/Fm), finally
Each achievement data is subjected to SPSS data analyses.
Statistic analysis result is as follows：
（1）Plant height
As shown in Table 1, the plant height of 30 kinds of Wild groundcover plants changes with the difference of shading rate.Kincup, wood are blue, hoof leaf bag
I, cold hole orchid, Du Ruo, Viola variegata, the wild plant height for fanning the plants such as flower, asarum, Dysosma versipellis, the Japanese ardisia, A.humulifoliavar. Heterophylla with
The increase of shading rate and the trend of growth is presented, plant height reaches maximum under S3 environment, and grows fine, and illustrates these plants
Object shade tolerance is stronger.Fevervine, climbing groundsel, clerodendron trichotomum, blood circulation promoting pill, dewdrop grass, the plant height of plants such as harvest in shading treatment
Under do not show significant difference, it may be possible to these wild plant species for illumination coerce adapt to regulating power it is stronger,
The expression of gene growth factor is not exclusively controlled by illumination stress.Blood circulation promoting pill, dewdrop grass and ground harvest be sprawl growth plant,
The ground growth of its branch patch, so difference is little in plant height, therefore plant height this character is not suitable as evaluation sprawl growth and plants
The index of object shade tolerance.
Influence of the different shading treatments of table 1 to plant plant height
（2）Leaf area
The variation of the single leaf leaf area of analysis is conducive to the variation of accurate description Leaf pattern, and as shown in Table 2-4, kincup, wood are blue, hoof leaf
Farfugium kaemferi, cold hole orchid, Du Ruo, Viola variegata, it is wild fan flower, asarum, Dysosma versipellis, the Japanese ardisia, A.humulifoliavar. Heterophylla, blood circulation promoting pill, harvest plant
Object full exposure, S1, S2 to S3 shade gradient under, reduced with intensity of illumination, the leaf area of plant leaf blade is presented successively substantially
Increased trend illustrates these plants in shading, and the growth of blade is influenced little by illumination stress.Uncinata Spikemoss Herb, chicken
Swear that the plants such as rattan, climbing groundsel, clerodendron trichotomum, dewdrop grass are increased in shading degree, plant leaf area does not occur bright
Aobvious variation illustrates that it is stronger to the adaptability of environment, can normal growth under the conditions of full exposure and shading.
Influence of the different shading treatments of table 2 to plant leaf area
（3）Chlorophyll content
The photosensitizer important as photosynthesis, the content and photosynthesis of chlorophyll are closely related, and content and ratio are
Plant to the important indicator of environmental suitability and producing level and maintain plant normal light cooperation use and leaf color,
The leading indicator of coloured silkization degree.As shown in table 3, chlorophyll content of the different plants under identical intensity of illumination is variant, same to phase
With chlorophyll content of the plant under different illumination intensity, there is also differences.Kincup, wooden indigo plant, ligularia fischeri, cold hole orchid, Du
If, Viola variegata, wild fan flower, asarum, Dysosma versipellis, the Japanese ardisia, A.humulifoliavar. Heterophylla, blood circulation promoting pill, harvest the chlorophyll content of plant
It is raised with the reduction of intensity of illumination, illustrates that the light levels needed for the Chlorophyll synthesis of these plants are low, shape under low light environment
Into a large amount of chlorophyll.The plants such as fevervine, climbing groundsel, clerodendron trichotomum, dewdrop grass chlorophyll in the case where intensity of illumination changes
Content difference is smaller, shows stronger adaptability.
The chlorophyll content of plant under the different shading treatments of table 3
（4）Chlorophyll fluorescence parameters
Chlorophyll fluorescence parameters under the full light of most plants blade are minimum, with the exacerbation of Shading levels, chlorophyll fluorescence parameters base
Originally raised trend successively is showed, is the performance that plant adapts to low light environment.Plant chlorophyll fluorescence index under shade increases
Add more, shade tolerance is stronger.If table 4 is understood, with the reduction of intensity of illumination, the chlorophyll fluorescence performance index of plant leaf blade is sent out
Raw certain variation.But different plant chlorophyll fluorescence property index variation trend are different.It is kincup, wooden indigo plant, ligularia fischeri, cold
Hole orchid, Du Ruo, Viola variegata, it is wild fan flower, asarum, Dysosma versipellis, the Japanese ardisia, A.humulifoliavar. Heterophylla, blood circulation promoting pill, the plant leaf blades such as harvest
Chlorophyll fluorescence parameters are raised with the reduction of light intensity, illustrate the very capable of these types of plant low light tolerance.
The chlorophyll fluorescence performance index of plant compares under the different shading treatments of table 4
（5）Wet-proof plants foliage filter screening result
According to green to plant height of 30 kinds of Wild groundcover plants under the conditions of different shading treatments, leaf area, chlorophyll content and leaf
30 kinds of plants are divided into 4 kinds of shade tolerant types by the measure of the indexs such as plain fluorescence index, be respectively strong shade tolerant, half shade tolerant, intolerant to
Cloudy, illumination insensitiveness（Or osculant）, the shade tolerant classification of type of 30 kinds of Wild groundcover plants is shown in Table 5, and the plant of strong shade tolerant type has
13 kinds, the plant of half shade tolerant type has 8 kinds, and the plant of shade tolerant does not have 5 kinds, and day neutral plant has 4 kinds.The donkey screened
The strong illumination neutral plant such as shade plant and fevervine such as hoof grass can be developed into is applied to afforestation for resistance to female ground cover plant
In construction.
The shade tolerant type of 5 30 kinds of Wild groundcover plants of table
| Shade tolerant type | Botanical name |
| Strong shade tolerant | Kincup, wooden indigo plant, ligularia fischeri, cold hole orchid, Du Ruo, Viola variegata, open country fan flower, asarum, Dysosma versipellis, the Japanese ardisia, A.humulifoliavar. Heterophylla, blood circulation promoting pill, harvest |
| Half shade tolerant | Uncinata Spikemoss Herb, the cold certain kind of berries, grass of meadow rue, strawberry cinquefoil root, white dead nettle, acanthaceous indigo, dianthus superbus, Pteris multifida |
| Not shade tolerant | Geranium wilfordii, awns beanstalk, smartweed, Japanese polygala, precious tongued bell grass |
| Illumination insensitiveness | Fevervine, climbing groundsel, clerodendron trichotomum, dewdrop grass |
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention, although
The present invention is described in detail with reference to the foregoing embodiments, it for those skilled in the art, still can be right
Technical solution recorded in previous embodiment modifies or equivalent substitution is carried out to which part technical characteristic, all at this
Within the spirit and principle of invention, any modifications, equivalent replacements and improvements are made should be included in the protection model of the present invention
Within enclosing.
Claims (5)
1. a kind of screening technique of resistance to female ground cover plant, comprises the following steps：
（1）Select to test garden：It should select to have good geographical position, physical features is smooth, has abundant water resources, and irrigation condition is good, well-ventilated,
With doing garden, build greenhouse in plot away from pollution sources；
（2）Test block division：Each growing area block is divided according to plant material grown feature and experimental program, including maternal original seed
Area, maternal breeding area, screening test area, and turned over, raked the soil level, ridging and bedding；
（3）Vegetable material prepares：To avoid the difference between plant individual, acquisition length is consistent from female parent, thickness is consistent, raw
The consistent cuttings of long situation carries out cuttage, breeds the consistent experiment material of a collection of growing way；
（4）Planting：After 30 ~ 45 d of cuttage, the indefinite root system of base portion in great numbers, cauline leaf well-grown, same breed growing way are selected
Consistent vegetable material is planted in screening test area；
（5）Shading treatment：Different shading treatments is realized by building shading net outside greenhouse；
（6）Management：Temperature, humidity, water-fertilizer nutrient are consistent in Control experiment area, other external factor are avoided to cause experiment larger
It influences, it is 15~28 DEG C to control temperature range, humidity range 60% ~ 80%, and liquid fertilizer sprayed once, sprays weekly 75,%00 bacterium weekly
1000 times of liquid of clear 1500 times of liquid of wettable powder or 80% carbendazol wettable powder fungicide are once；
（7）Shade tolerant merit rating：After shading treatment 4 months, the relevant form index and physical signs of each vegetable material are measured,
The shade tolerant ability of comprehensive analysis and judgement plant filters out the strong plant variety of shade tolerance.
2. the screening technique of a kind of resistance to female ground cover plant according to claim 1, which is characterized in that cuttings is from 1 year
The brachyplast item for 6 ~ 10 cm being cut on branch is given birth to, at least 1 is saved in each cuttings, removes morphology lower leaflet, retains shape
2-3 fresh blades, with 80% mancozeb wettable powder, 800 times of 1 ~ 2 min of liquid disinfectant, are then immersed in 100 above state
1 ~ 2 h in No. 1 solution of ABT root-inducing powders of mg/kg, cuttage are vermiculite in volume ratio：Perlite：Nutrition Soil=1:1:2 cultivation base
In matter, cuttings embedded depth is spike length 1/3 ~ 1/2.
A kind of 3. screening technique of resistance to female ground cover plant according to claim 1, which is characterized in that step（4）Middle sieve
Trial zone planting depth is selected in 15 ~ 20 cm, plantation spacing is in 40 ~ 50 cm.
A kind of 4. screening technique of resistance to female ground cover plant according to claim 1, which is characterized in that step（5）In not
It is that different degrees of shading net is built outside greenhouse with shading treatment, it is 0%, 30%, 50% and 80% to realize shading rate.
A kind of 5. screening technique of resistance to female ground cover plant according to claim 1, which is characterized in that step（7）Middle shape
State index includes plant height and leaf area, and physical signs includes chlorophyll content and chlorophyll fluorescence performance index, passes through SPSS numbers
According to analysis, compare same plant under different shading treatments under the situation of change of biological indicator and different shading treatments not
With the variation tendency of same biological indicator between plant.
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| CN115067206A (en) * | 2022-07-22 | 2022-09-20 | 山西农业大学园艺学院 | Screening method and screening device for saline-alkali tolerant garden ground cover plants |
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Application publication date: 20180605 |