CN115067206A - Screening method and screening device for saline-alkali tolerant garden ground cover plants - Google Patents
Screening method and screening device for saline-alkali tolerant garden ground cover plants Download PDFInfo
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- 239000003513 alkali Substances 0.000 title claims abstract description 138
- 238000012216 screening Methods 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 61
- 238000004088 simulation Methods 0.000 claims abstract description 115
- 239000002689 soil Substances 0.000 claims abstract description 54
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims description 305
- 238000012545 processing Methods 0.000 claims description 48
- 108700001094 Plant Genes Proteins 0.000 claims description 47
- 238000004364 calculation method Methods 0.000 claims description 46
- 238000009966 trimming Methods 0.000 claims description 46
- 238000011156 evaluation Methods 0.000 claims description 42
- 238000005259 measurement Methods 0.000 claims description 24
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 21
- 230000004083 survival effect Effects 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 9
- 230000008635 plant growth Effects 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 230000005080 plant death Effects 0.000 claims description 4
- 239000002362 mulch Substances 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 2
- 238000012364 cultivation method Methods 0.000 claims 2
- 238000013138 pruning Methods 0.000 claims 2
- 230000002068 genetic effect Effects 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000011534 incubation Methods 0.000 abstract 1
- 238000005728 strengthening Methods 0.000 description 9
- 238000011161 development Methods 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/1225—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold or salt resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Abstract
The invention relates to the technical field of screening methods of garden ground cover plants, in particular to a screening method and a screening device of saline-alkali tolerant garden ground cover plants, and provides the following scheme aiming at the problems of low success rate of screening the saline-alkali tolerant plants caused by simple screening process and undefined screening directivity in the current screening method technology of the garden ground cover plants, wherein the screening method of the saline-alkali tolerant garden ground cover plants comprises the following steps: s1: preparation, S2: environmental simulation, S3: incubation, S4: the invention aims to screen by simulating a soil environment, effectively avoids screening failure caused by the difference between an experimental environment and a field environment, screens out plants more suitable for a saline-alkali soil environment in a natural state, screens the plants aiming at saline-alkali resistance by determining the screening directivity, and improves the screening success rate.
Description
Technical Field
The invention relates to the technical field of screening methods of garden ground cover plants, in particular to a screening method and a screening device of salt and alkali resistant garden ground cover plants.
Background
In recent years, China strengthens the protection of wild garden plants, establishes a plurality of ecological forest parks, and makes protective measures for endangered wild plants, aiming at strengthening the protection of wild plant resources. The method has important significance for enriching the existing landscape plant communities by screening wild landscape plants in various places and promoting the balanced development of landscape plant ecological communities. Through the screening to wild landscape plant and carry out reasonable configuration, be the construction ecological gardens, maintain ecological balance's important means, both played landscape's effect, richened landscape plant's kind again, also be the important method that accords with landscape plant diversified development requirement. However, when screening plants, a scientific method is used, and the characteristics of plant growth are combined, and a certain principle is followed, so that the development direction of wild garden plant configuration is the direction.
However, the existing screening method and screening device for saline-alkali tolerant landscape cover plants have the problems that the screening process is simple, the screening directionality is not clear, and the success rate of screening the saline-alkali tolerant landscape cover plants is low, so that the screening method and screening device for the saline-alkali tolerant landscape cover plants are provided for solving the problems.
Disclosure of Invention
The invention aims to solve the problems that the screening process is simple, the screening directivity is not clear, the success rate of screening out saline-alkali tolerant plants is low and the like in the existing screening method technology of the garden cover plants, and provides a screening method and a screening device of the saline-alkali tolerant garden cover plants.
In order to achieve the purpose, the invention adopts the following technical scheme:
a screening method of saline-alkali tolerant garden mulch plants comprises the following steps:
s1: preparation is carried out: selecting the types of plant growing soil and garden ground cover plants, and determining the initial biological characteristics of the selected ground cover plants;
s2: environmental simulation: a professional simulates a saline-alkali environment in the simulation module, and plants are planted in the ground cover;
s3: and (3) cultivating: after the ground cover plants are planted, the ground cover plants are artificially cultivated through the cultivation module;
s4: screening is carried out: screening out plants with the best saline-alkali resistance effect as plant genes by professionals, and carrying out large-scale propagation on the screened plant genes;
preferably, in S1, a saline-alkali soil is selected as a plant growth soil, and meanwhile, a professional selects a robust landscape cover plant growing in a normal environment to plant, and determines an initial biological characteristic of the selected cover plant, wherein the initial biological characteristic includes root length, stem length, leaf length, plant height and leaf number, and the initial biological characteristic of the selected cover plant needs to be kept consistent;
preferably, in S2, the saline-alkali environment is simulated in the simulation module by professional, wherein the simulation module includes a plurality of simulation zones, and the saline-alkali degree of each simulation zone is different, wherein the saline-alkali degree of each zone is preset by professional, and when preset, the professional calculates the saline-alkali degree threshold according to the selected species of the ground cover plants, and selects gradient data of saline-alkali degree from the threshold obtained by calculation through the calculation module, and prepares saline-alkali soil through the selected gradient data of saline-alkali degree, puts the prepared saline-alkali low soil into the corresponding simulation zone, pastes a data label of saline-alkali degree outside the simulation zone, plants the selected ground cover plants are planted in different simulation zones according to planting requirements, and marks the planted plants, after planting is finished, the living environment and field conditions required by the selected ground cover plants are controlled through the control module;
preferably, in S3, after the vegetation is planted, the vegetation is artificially cultivated by the cultivating module, wherein the existing cultivating method is adopted during cultivation, a uniform cultivating method is adopted in all simulation intervals, and during cultivation, root conditions and seedling height data of all the vegetation planted in the simulation intervals are measured by the measuring module, wherein the cultivating time of the vegetation before the first measurement is started is not less than 2 weeks, and the measuring period is 7 days, after one measurement is completed, the measurement result is judged by the judging module, and the judgment result is processed by the processing module, wherein the measurement result is that the vegetation is alive when the root system is elongated and the seedling height data is increased, the vegetation is alive when the root system is elongated and the seedling height data is not increased, and the rest measurement results are dead, if the plants are judged to survive, the surviving plants are continuously cultivated, the measured data are recorded at the corresponding plant numbers, if the plants are judged to die, the dead plants are eradicated, and meanwhile, the plant survival rate of each simulation interval is calculated through a calculation module;
preferably, in S4, a professional draws a line graph of all measurement data recorded by the same surviving plant through a drawing module, compares the drawn line graph, selects a plant with the strongest stability in the same simulation interval as a plant gene preselection by comparing the comparison result, evaluates all the preselected plant genes through an evaluation module, screens out a plant with the best saline-alkali resistance as a plant gene through evaluation, and performs scale propagation on the screened plant gene, wherein a stem tip and anthers on the screened plant gene need to be trimmed through a trimming module before performing scale propagation, after trimming, the screened plant gene needs to be cut into small plants with the same size and soaked in an alcohol solution with a concentration of 70% for 10-30 seconds, and after soaking, the trimmed small plants are respectively inoculated into an initial culture medium through a culture module for culture, performing real-time observation by professionals during culture, and processing through observation results, wherein the observation results show that cells start to differentiate to form callus, performing subculture, the observation results show that the cells start to differentiate to form callus, continuing to perform culture, performing real-time observation by the professionals during the culture process until the observation results show that the cells start to differentiate to form callus, stopping observation, and selecting half of the plants by the professionals after the subculture is completed to perform strong seedling treatment, wherein the strong seedling treatment is to place the cultured plants outdoors for culture, plant the plants in the saline-alkali soil after the plants are adapted to the external environment, and simultaneously select the other half of the plants for secondary large-scale propagation;
a screening device for saline-alkali tolerant garden cover plants comprises a simulation module, a calculation module, a control module, a cultivation module, a determination module, a judgment module, a processing module, a drawing module, an evaluation module, a trimming module and a cultivation module, wherein the simulation module is connected with the calculation module, the calculation module is connected with the control module, the control module is connected with the cultivation module, the cultivation module is connected with the determination module, the determination module is connected with the judgment module, the judgment module is connected with the processing module, the processing module is connected with the drawing module, the drawing module is connected with the evaluation module, the evaluation module is connected with the trimming module, and the trimming module is connected with the cultivation module;
preferably, the simulation module is used for simulating the soil condition of the saline-alkali soil, the calculation module is used for calculating the saline-alkali degree threshold value of the selected ground cover plant species, the control module is used for controlling the living environment and the field condition required by the selected ground cover plant, the cultivation module is used for cultivating the planted ground cover plant, the determination module is used for determining the root system condition and seedling height data of all ground cover plants planted in the simulation interval, the determination module is used for determining the determination result, the processing module is used for processing the determination result, the drawing module is used for drawing a line graph of all determination data recorded by the same survival plant, the evaluation module is used for evaluating all pre-selected gene plants, and the trimming module is used for screening trimmed plant genes, the culture module is used for large-scale propagation culture.
Compared with the prior art, the invention has the beneficial effects that:
1. screening is carried out by simulating the soil environment, so that the screening failure caused by the difference between the experimental environment and the field environment is effectively avoided, and plants more suitable for the saline-alkali soil environment in the natural state are screened.
2. Through the directive property of making clear the screening, screen to resistant saline and alkaline performance to the plant, improved the success rate of screening.
The invention aims to effectively avoid the screening failure caused by the difference between the experimental environment and the field environment by screening through simulating the soil environment, screen out the plants more suitable for the saline-alkali soil environment in the natural state, and screen out the plants aiming at the saline-alkali resistance through determining the screening directivity, thereby improving the screening success rate.
Drawings
FIG. 1 is a flow chart of a method for screening saline-alkali tolerant landscape cover plants according to the present invention;
fig. 2 is a block diagram of a screening device for saline-alkali tolerant landscape cover plants according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example one
Referring to fig. 1, a method for screening saline-alkali tolerant landscape cover plants, comprising the steps of:
s1: preparation is carried out: selecting saline-alkali soil as plant growth soil, simultaneously selecting strong garden ground cover plants growing in a normal environment by professionals for planting, and determining the initial biological characteristics of the selected ground cover plants, wherein the initial biological characteristics comprise root length, stem length, leaf length, plant height and leaf number, and the initial biological characteristics of the selected ground cover plants need to be kept consistent;
s2: environmental simulation: the saline-alkali environment is simulated in a simulation module by a professional, wherein the simulation module comprises a plurality of simulation intervals, the saline-alkali degree of each simulation interval is different, wherein the saline-alkali degree of each section is preset by professional personnel, and the professional personnel calculates the saline-alkali degree threshold value according to the selected variety of vegetation plants when the saline-alkali degree is preset, the saline-alkali degree gradient data is selected through the threshold value interval obtained by calculation through the calculation module, the saline-alkali soil is prepared through the selected saline-alkali degree gradient data by professional personnel, the prepared saline-alkali low soil is placed in the corresponding simulation interval, a saline-alkali degree data label is pasted outside the simulation interval, meanwhile, the selected ground cover plants are planted in different simulation intervals according to the planting requirements, the planted plants are labeled, after planting is finished, the living environment and field conditions required by the selected ground cover plants are controlled through the control module;
s3: and (3) cultivating: cultivating the ground cover plants through a cultivating module by manpower after the ground cover plants are planted, wherein the existing cultivating method is adopted during cultivation, a uniform cultivating method is adopted in all simulation intervals, root system conditions and seedling height data of all ground cover plants planted in the simulation intervals are measured through a measuring module in the cultivating process, the cultivating time of the ground cover plants before the first measurement is started is not less than 2 weeks, the measuring period is 7 days, the measuring result is judged through a judging module after one measurement is finished, the judging result is processed through a processing module, the measuring result is that the plants survive if the root system has an extension phenomenon and the seedling height data is increased, the plants survive if the root system has the extension phenomenon and the seedling height data is not increased, the rest measuring results are all judged to be plant death, and the surviving plants are continuously cultivated if the plants survive, recording the measured data at the corresponding plant number, removing dead plants if the plants are judged to be dead, and calculating the plant survival rate of each simulation interval through a calculation module;
s4: screening is carried out: the method comprises the steps that a professional draws a line graph through a drawing module according to all measured data recorded by the same surviving plant, compares the drawn line graph, selects the plant with the strongest stability in the same simulation interval as a plant gene preselection through a comparison result, evaluates all the gene preselection plants through an evaluation module, screens out the plant with the best saline-alkali resistance effect as the plant gene through evaluation, and carries out large-scale propagation on the screened plant gene, wherein before the large-scale propagation, a stem tip and anther on the screened plant gene need to be trimmed through a trimming module, the screened plant gene needs to be cut into small plants with the same size after the trimming is finished, and the small plants are soaked in an alcohol solution with the concentration of 70% for 10-30 seconds, and are respectively inoculated into a primary culture medium through a culture module for culture after the soaking is finished, the method comprises the following steps of performing real-time observation by professionals during culture, and processing through observation results, wherein the observation results show that cells start to differentiate to form callus, performing subculture, the observation results show that the cells start to differentiate to form callus, continuing to culture, performing real-time observation by the professionals during the culture process until the observation results show that the cells start to differentiate to form callus, stopping observation, and selecting half of plants by the professionals after the subculture is completed to perform seedling strengthening treatment, wherein the seedling strengthening treatment is to place the cultured plants outdoors for culture, plant the plants in saline-alkali soil after the plants are adapted to the external environment, and simultaneously select the other half of plants for secondary large-scale propagation;
referring to fig. 2, a screening device for saline-alkali tolerant garden cover plants comprises a simulation module, a calculation module, a control module, a cultivation module, a determination module, a judgment module, a processing module, a drawing module, an evaluation module, a trimming module and a cultivation module, wherein the simulation module is connected with the calculation module, the calculation module is connected with the control module, the control module is connected with the cultivation module, the cultivation module is connected with the determination module, the determination module is connected with the judgment module, the judgment module is connected with the processing module, the processing module is connected with the drawing module, the drawing module is connected with the evaluation module, the evaluation module is connected with the trimming module, the trimming module is connected with the cultivation module, the simulation module is used for simulating soil conditions of saline-alkali soil, the calculation module is used for calculating saline-alkali degree threshold value of selected ground cover plant species, and the control module is used for controlling living environment and field conditions required by the selected ground cover plants, the cultivation module is used for cultivating planted ground cover plants, the determination module is used for determining root conditions and seedling height data of all ground cover plants planted in the simulation interval, the determination module is used for determining determination results, the processing module is used for processing the determination results, the drawing module is used for drawing line graphs of all determination data recorded by the same survival plant, the evaluation module is used for evaluating all gene preselected plants, the trimming module is used for trimming screened plant genes, and the cultivation module is used for large-scale propagation expanding cultivation.
Example two
Referring to fig. 1, a method for screening saline-alkali tolerant landscape cover plants, comprising the steps of:
s1: preparation is carried out: selecting saline-alkali soil as plant growth soil, simultaneously selecting strong garden cover plants growing in a normal environment by professionals for planting, and determining the initial biological characteristics of the selected cover plants;
s2: environmental simulation: the saline-alkali environment is simulated in a simulation module by a professional, wherein the simulation module comprises a plurality of simulation intervals, the saline-alkali degree of each simulation interval is different, wherein the salinity and alkalinity degree of each section is preset by professional personnel, and the professional personnel calculates the salinity and alkalinity degree threshold value according to the selected variety of the vegetation plants when the salinity and alkalinity degree is preset, the saline-alkali degree gradient data is selected through the threshold value interval obtained by calculation through the calculation module, the saline-alkali soil is prepared through the selected saline-alkali degree gradient data by professional personnel, the prepared saline-alkali low soil is placed in the corresponding simulation interval, a saline-alkali degree data label is pasted outside the simulation interval, meanwhile, the selected ground cover plants are planted in different simulation intervals according to the planting requirements, the planted plants are labeled, after planting, the living environment and field conditions required by the selected ground cover plants are controlled through the control module;
s3: and (3) cultivating: cultivating the ground cover plants through a cultivating module by manpower after the ground cover plants are planted, wherein the existing cultivating method is adopted during cultivation, a uniform cultivating method is adopted in all simulation intervals, root system conditions and seedling height data of all ground cover plants planted in the simulation intervals are measured through a measuring module in the cultivating process, the cultivating time of the ground cover plants before the first measurement is started is not less than 2 weeks, the measuring period is 7 days, the measuring result is judged through a judging module after one measurement is finished, the judging result is processed through a processing module, the measuring result is that the plants survive if the root system has an extension phenomenon and the seedling height data is increased, the plants survive if the root system has the extension phenomenon and the seedling height data is not increased, the rest measuring results are all judged to be plant death, and the surviving plants are continuously cultivated if the plants survive, recording the measured data at the corresponding plant number, removing dead plants if the plants are judged to be dead, and calculating the plant survival rate of each simulation interval through a calculation module;
s4: screening is carried out: drawing a line graph of all measured data recorded by the same surviving plant through a drawing module by a professional, comparing the drawn line graph, selecting the plant with the strongest stability in the same simulation interval as a plant gene preselection through a comparison result, evaluating all the preselection plants of the gene through an evaluation module by the professional, screening out the plant with the best saline-alkali resistance effect as the plant gene through evaluation, and carrying out large-scale propagation on the screened plant gene, wherein the stem tip and anther on the screened plant gene need to be trimmed through a trimming module before the large-scale propagation is carried out, the screened plant gene needs to be cut into small plants with the same size after the trimming is finished, and the small plants are soaked in an alcohol solution with the concentration of 70% for 10-30 seconds, and the cut small plants are respectively inoculated into a primary culture medium through a culture module for culture after the soaking is finished, the method comprises the following steps of performing real-time observation by professionals during culture, and processing through observation results, wherein the observation results show that cells start to differentiate to form callus, performing subculture, the observation results show that the cells start to differentiate to form callus, continuing to culture, performing real-time observation by the professionals during the culture process until the observation results show that the cells start to differentiate to form callus, stopping observation, and selecting half of plants by the professionals after the subculture is completed to perform seedling strengthening treatment, wherein the seedling strengthening treatment is to place the cultured plants outdoors for culture, plant the plants in saline-alkali soil after the plants are adapted to the external environment, and simultaneously select the other half of plants for secondary large-scale propagation;
referring to fig. 2, a screening device for saline-alkali tolerant garden cover plants comprises a simulation module, a calculation module, a control module, a cultivation module, a determination module, a judgment module, a processing module, a drawing module, an evaluation module, a trimming module and a cultivation module, wherein the simulation module is connected with the calculation module, the calculation module is connected with the control module, the control module is connected with the cultivation module, the cultivation module is connected with the determination module, the determination module is connected with the judgment module, the judgment module is connected with the processing module, the processing module is connected with the drawing module, the drawing module is connected with the evaluation module, the evaluation module is connected with the trimming module, the trimming module is connected with the cultivation module, the simulation module is used for simulating soil conditions of saline-alkali soil, the calculation module is used for calculating saline-alkali degree threshold value of selected ground cover plant species, and the control module is used for controlling living environment and field conditions required by the selected ground cover plants, the cultivation module is used for cultivating planted ground cover plants, the determination module is used for determining root conditions and seedling height data of all ground cover plants planted in the simulation interval, the determination module is used for determining determination results, the processing module is used for processing the determination results, the drawing module is used for drawing line graphs of all determination data recorded by the same survival plant, the evaluation module is used for evaluating all gene preselected plants, the trimming module is used for trimming screened plant genes, and the cultivation module is used for large-scale propagation expanding cultivation.
EXAMPLE III
Referring to fig. 1, a method for screening saline-alkali tolerant landscape cover plants, comprising the steps of:
s1: preparation is carried out: selecting saline-alkali soil as plant growth soil, simultaneously selecting strong garden ground cover plants growing in a normal environment by professionals for planting, and determining the initial biological characteristics of the selected ground cover plants, wherein the initial biological characteristics comprise root length, stem length, leaf length, plant height and leaf number, and the initial biological characteristics of the selected ground cover plants need to be kept consistent;
s2: environmental simulation: carrying out saline-alkali environment simulation in a simulation module by professionals, wherein the simulation module comprises a plurality of simulation intervals, the saline-alkali degree of each simulation interval is different, the saline-alkali degree of each interval is preset by the professionals, the configured saline-alkali low soil is placed in the corresponding simulation interval, a saline-alkali degree data label is pasted outside the simulation interval, meanwhile, the selected ground cover plants are planted in different simulation intervals according to planting requirements, the planted plants are labeled, and after the planting is finished, the living environment and field conditions required by the selected ground cover plants are controlled through a control module;
s3: and (3) cultivating: cultivating the ground cover plants through a cultivating module manually after the ground cover plants are planted, wherein the existing cultivating method is adopted during cultivation, a uniform cultivating method is adopted in all simulation intervals, root system conditions and seedling height data of all the ground cover plants planted in the simulation intervals are measured through a measuring module in the cultivating process, the cultivating time of the ground cover plants before the first measurement is started is not less than 2 weeks, the measuring period is 7 days, the measuring result is judged through a judging module after one measurement is finished, the judging result is processed through a processing module, the measuring result is that the root system is prolonged and the seedling height data is increased, the plants are judged to survive, the root system is prolonged, the seedling height data is not increased, the rest measuring results are all judged to be dead, and the surviving plants are continuously cultivated if the plants are judged to survive, recording the measured data at the corresponding plant number, removing dead plants if the plants are judged to be dead, and calculating the plant survival rate of each simulation interval through a calculation module;
s4: screening is carried out: drawing a line graph of all measured data recorded by the same surviving plant through a drawing module by a professional, comparing the drawn line graph, selecting the plant with the strongest stability in the same simulation interval as a plant gene preselection through a comparison result, evaluating all the preselection plants of the gene through an evaluation module by the professional, screening out the plant with the best saline-alkali resistance effect as the plant gene through evaluation, and carrying out large-scale propagation on the screened plant gene, wherein the stem tip and anther on the screened plant gene need to be trimmed through a trimming module before the large-scale propagation is carried out, the screened plant gene needs to be cut into small plants with the same size after the trimming is finished, and the small plants are soaked in an alcohol solution with the concentration of 70% for 10-30 seconds, and the cut small plants are respectively inoculated into a primary culture medium through a culture module for culture after the soaking is finished, the method comprises the following steps of performing real-time observation by professionals during culture, and processing through observation results, wherein the observation results show that cells start to differentiate to form callus, performing subculture, the observation results show that the cells start to differentiate to form callus, continuing to culture, performing real-time observation by the professionals during the culture process until the observation results show that the cells start to differentiate to form callus, stopping observation, and selecting half of plants by the professionals after the subculture is completed to perform seedling strengthening treatment, wherein the seedling strengthening treatment is to place the cultured plants outdoors for culture, plant the plants in saline-alkali soil after the plants are adapted to the external environment, and simultaneously select the other half of plants for secondary large-scale propagation;
referring to fig. 2, a screening device for saline-alkali tolerant garden cover plants comprises a simulation module, a calculation module, a control module, a cultivation module, a determination module, a judgment module, a processing module, a drawing module, an evaluation module, a trimming module and a cultivation module, wherein the simulation module is connected with the calculation module, the calculation module is connected with the control module, the control module is connected with the cultivation module, the cultivation module is connected with the determination module, the determination module is connected with the judgment module, the judgment module is connected with the processing module, the processing module is connected with the drawing module, the drawing module is connected with the evaluation module, the evaluation module is connected with the trimming module, the trimming module is connected with the cultivation module, the simulation module is used for simulating soil conditions of saline-alkali soil, the calculation module is used for calculating saline-alkali degree threshold value of selected ground cover plant species, and the control module is used for controlling living environment and field conditions required by the selected ground cover plants, the cultivation module is used for cultivating planted ground cover plants, the determination module is used for determining root conditions and seedling height data of all ground cover plants planted in the simulation interval, the determination module is used for determining determination results, the processing module is used for processing the determination results, the drawing module is used for drawing line graphs of all determination data recorded by the same survival plant, the evaluation module is used for evaluating all gene preselected plants, the trimming module is used for trimming screened plant genes, and the cultivation module is used for large-scale propagation expanding cultivation.
Example four
Referring to fig. 1, a method for screening saline-alkali tolerant landscape cover plants, comprising the steps of:
s1: preparation is carried out: selecting saline-alkali soil as plant growth soil, simultaneously selecting strong garden ground cover plants growing in a normal environment by professionals for planting, and determining the initial biological characteristics of the selected ground cover plants, wherein the initial biological characteristics comprise root length, stem length, leaf length, plant height and leaf number, and the initial biological characteristics of the selected ground cover plants need to be kept consistent;
s2: environmental simulation: the saline-alkali environment is simulated in a simulation module by a professional, wherein the simulation module comprises a plurality of simulation intervals, the saline-alkali degree of each simulation interval is different, wherein the saline-alkali degree of each section is preset by professional personnel, and the professional personnel calculates the saline-alkali degree threshold value according to the selected variety of vegetation plants when the saline-alkali degree is preset, the saline-alkali degree gradient data is selected through the threshold value interval obtained by calculation through the calculation module, the saline-alkali soil is prepared through the selected saline-alkali degree gradient data by professional personnel, the prepared saline-alkali low soil is placed in the corresponding simulation interval, a saline-alkali degree data label is pasted outside the simulation interval, meanwhile, the selected ground cover plants are planted in different simulation intervals according to the planting requirements, the planted plants are labeled, after planting is finished, the living environment and field conditions required by the selected ground cover plants are controlled through the control module;
s3: and (3) cultivating: cultivating the ground cover plants through a cultivating module manually after the ground cover plants are planted, wherein the existing cultivating method is adopted during cultivation, a uniform cultivating method is adopted in all simulation intervals, root system conditions and seedling height data of all ground cover plants planted in the simulation intervals are measured through a measuring module in the cultivating process, the cultivating time of the ground cover plants before the first measurement is started is not less than 2 weeks, the measuring period is 7 days, the measuring result is judged through a judging module after one measurement is finished, and the judging result is processed through a processing module;
s4: and (3) screening: drawing a line graph of all measured data recorded by the same surviving plant through a drawing module by a professional, comparing the drawn line graph, selecting the plant with the strongest stability in the same simulation interval as a plant gene preselection through a comparison result, evaluating all the preselection plants of the gene through an evaluation module by the professional, screening out the plant with the best saline-alkali resistance effect as the plant gene through evaluation, and carrying out large-scale propagation on the screened plant gene, wherein the stem tip and anther on the screened plant gene need to be trimmed through a trimming module before the large-scale propagation is carried out, the screened plant gene needs to be cut into small plants with the same size after the trimming is finished, and the small plants are soaked in an alcohol solution with the concentration of 70% for 10-30 seconds, and the cut small plants are respectively inoculated into a primary culture medium through a culture module for culture after the soaking is finished, the method comprises the following steps of performing real-time observation by professionals during culture, and processing through observation results, wherein the observation results show that cells start to differentiate to form callus, performing subculture, the observation results show that the cells start to differentiate to form callus, continuing to culture, performing real-time observation by the professionals during the culture process until the observation results show that the cells start to differentiate to form callus, stopping observation, and selecting half of plants by the professionals after the subculture is completed to perform seedling strengthening treatment, wherein the seedling strengthening treatment is to place the cultured plants outdoors for culture, plant the plants in saline-alkali soil after the plants are adapted to the external environment, and simultaneously select the other half of plants for secondary large-scale propagation;
referring to fig. 2, a screening device for saline-alkali tolerant garden cover plants comprises a simulation module, a calculation module, a control module, a cultivation module, a determination module, a judgment module, a processing module, a drawing module, an evaluation module, a trimming module and a cultivation module, wherein the simulation module is connected with the calculation module, the calculation module is connected with the control module, the control module is connected with the cultivation module, the cultivation module is connected with the determination module, the determination module is connected with the judgment module, the judgment module is connected with the processing module, the processing module is connected with the drawing module, the drawing module is connected with the evaluation module, the evaluation module is connected with the trimming module, the trimming module is connected with the cultivation module, the simulation module is used for simulating soil conditions of saline-alkali soil, the calculation module is used for calculating saline-alkali degree threshold value of selected ground cover plant species, and the control module is used for controlling living environment and field conditions required by the selected ground cover plants, the cultivation module is used for cultivating planted ground cover plants, the determination module is used for determining root conditions and seedling height data of all ground cover plants planted in the simulation interval, the determination module is used for determining determination results, the processing module is used for processing the determination results, the drawing module is used for drawing line graphs of all determination data recorded by the same survival plant, the evaluation module is used for evaluating all gene preselected plants, the trimming module is used for trimming screened plant genes, and the cultivation module is used for large-scale propagation expanding cultivation.
EXAMPLE five
Referring to fig. 1, a method for screening saline-alkali tolerant landscape cover plants, comprising the steps of:
s1: preparation is carried out: selecting saline-alkali soil as plant growth soil, simultaneously selecting strong garden ground cover plants growing in a normal environment by professionals for planting, and determining the initial biological characteristics of the selected ground cover plants, wherein the initial biological characteristics comprise root length, stem length, leaf length, plant height and leaf number, and the initial biological characteristics of the selected ground cover plants need to be kept consistent;
s2: environmental simulation: the saline-alkali environment is simulated in a simulation module by a professional, wherein the simulation module comprises a plurality of simulation intervals, the saline-alkali degree of each simulation interval is different, wherein the saline-alkali degree of each section is preset by professional personnel, and the professional personnel calculates the saline-alkali degree threshold value according to the selected variety of vegetation plants when the saline-alkali degree is preset, the saline-alkali degree gradient data is selected through the threshold value interval obtained by calculation through the calculation module, the saline-alkali soil is prepared through the selected saline-alkali degree gradient data by professional personnel, the prepared saline-alkali low soil is placed in the corresponding simulation interval, a saline-alkali degree data label is pasted outside the simulation interval, meanwhile, the selected ground cover plants are planted in different simulation intervals according to the planting requirements, the planted plants are labeled, after planting is finished, the living environment and field conditions required by the selected ground cover plants are controlled through the control module;
s3: and (3) cultivating: cultivating the ground cover plants through a cultivating module by manpower after the ground cover plants are planted, wherein the existing cultivating method is adopted during cultivation, a uniform cultivating method is adopted in all simulation intervals, root system conditions and seedling height data of all ground cover plants planted in the simulation intervals are measured through a measuring module in the cultivating process, the cultivating time of the ground cover plants before the first measurement is started is not less than 2 weeks, the measuring period is 7 days, the measuring result is judged through a judging module after one measurement is finished, the judging result is processed through a processing module, the measuring result is that the plants survive if the root system has an extension phenomenon and the seedling height data is increased, the plants survive if the root system has the extension phenomenon and the seedling height data is not increased, the rest measuring results are all judged to be plant death, and the surviving plants are continuously cultivated if the plants survive, recording the measured data at the corresponding plant number, removing dead plants if the plants are judged to be dead, and calculating the plant survival rate of each simulation interval through a calculation module;
s4: screening is carried out: drawing a line graph of all measured data recorded by the same surviving plant through a drawing module by a professional, comparing the drawn line graph, selecting the plant with the strongest stability in the same simulation interval as a plant gene preselection through a comparison result, evaluating all the preselection plants of the gene through an evaluation module by the professional, screening out the plant with the best saline-alkali resistance effect as the plant gene through evaluation, and carrying out large-scale propagation on the screened plant gene, wherein the stem tip and anther on the screened plant gene need to be trimmed through a trimming module before the large-scale propagation is carried out, the screened plant gene needs to be cut into small plants with the same size after the trimming is finished, and the small plants are soaked in an alcohol solution with the concentration of 70% for 10-30 seconds, and the cut small plants are respectively inoculated into a primary culture medium through a culture module for culture after the soaking is finished, wherein, the culture is observed in real time by a professional and processed according to the observation result, wherein the observation result shows that the cells begin to differentiate to form callus, the subculture is carried out, the observation result shows that the cells begin to differentiate to form callus, the culture is continued, and the professional observes in real time in the culture process until the observation result shows that the cells begin to differentiate to form callus, and the observation is stopped;
referring to fig. 2, a screening device for saline-alkali tolerant garden cover plants comprises a simulation module, a calculation module, a control module, a cultivation module, a determination module, a judgment module, a processing module, a drawing module, an evaluation module, a trimming module and a cultivation module, wherein the simulation module is connected with the calculation module, the calculation module is connected with the control module, the control module is connected with the cultivation module, the cultivation module is connected with the determination module, the determination module is connected with the judgment module, the judgment module is connected with the processing module, the processing module is connected with the drawing module, the drawing module is connected with the evaluation module, the evaluation module is connected with the trimming module, the trimming module is connected with the cultivation module, the simulation module is used for simulating soil conditions of saline-alkali soil, the calculation module is used for calculating saline-alkali degree threshold value of selected ground cover plant species, and the control module is used for controlling living environment and field conditions required by the selected ground cover plants, the cultivation module is used for cultivating planted ground cover plants, the determination module is used for determining root conditions and seedling height data of all ground cover plants planted in the simulation interval, the determination module is used for determining determination results, the processing module is used for processing the determination results, the drawing module is used for drawing line graphs of all determination data recorded by the same survival plant, the evaluation module is used for evaluating all gene preselected plants, the trimming module is used for trimming screened plant genes, and the cultivation module is used for large-scale propagation expanding cultivation.
The screening method of the saline-alkali tolerant garden mulch plant in the first embodiment, the second embodiment, the third embodiment, the fourth embodiment and the fifth embodiment is tested, and the following results are obtained:
the screening methods of the saline-alkali tolerant garden cover plants prepared in the first embodiment, the second embodiment, the third embodiment, the fourth embodiment and the fifth embodiment have a significantly improved screening success rate compared with the prior art, and the first embodiment is the best embodiment.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. A screening method of saline-alkali tolerant garden mulch plants is characterized by comprising the following steps:
s1: preparation is carried out: selecting the types of plant growing soil and garden ground cover plants, and determining the initial biological characteristics of the selected ground cover plants;
s2: and (3) environment simulation: a professional simulates the saline-alkali environment in the simulation module, and plants are planted in the ground cover;
s3: and (3) cultivating: after the ground cover plants are planted, the ground cover plants are artificially cultivated through the cultivation module;
s4: screening is carried out: the professional selects the plant with the best saline-alkali tolerance effect as the plant gene, and performs large-scale propagation on the selected plant gene.
2. The method for screening haloalkali-tolerant landscape cover plants according to claim 1, wherein a saline-alkali soil is selected as a plant growth soil in the S1, and a professional selects a healthy landscape cover plant growing under a normal environment to plant, and determines the initial biological characteristics of the selected cover plant, wherein the initial biological characteristics include root length, stem length, leaf length, plant height and leaf number, and the initial biological characteristics of the selected cover plant are maintained to be uniform.
3. The method for screening saline-alkali tolerant garden cover plants according to claim 1, wherein in S2, a professional simulates a saline-alkali environment in a simulation module, wherein the simulation module comprises a plurality of simulation zones, and the saline-alkali degree of each simulation zone is different, wherein the saline-alkali degree of each zone is preset by the professional, and when the saline-alkali degree threshold is preset, the professional calculates the saline-alkali degree threshold according to the selected type of the garden cover plant, selects the gradient data of the saline-alkali degree from the threshold zone obtained by the calculation of the calculation module, prepares the saline-alkali soil according to the gradient data of the saline-alkali degree, puts the prepared saline-alkali low soil into the corresponding simulation zone, pastes a data label of the saline-alkali degree outside the simulation zone, and plants the selected garden cover plant according to the planting requirement in different simulation zones, and the planted plants are labeled, and the living environment and field conditions required by the selected ground cover plants are controlled through the control module after the planting is finished.
4. The method for screening saline-alkali tolerant landscape ground cover plants according to claim 1, wherein the ground cover plants are cultivated through the cultivation module manually after being planted in S3, wherein the cultivation is performed by using the existing cultivation method, and the unified cultivation method is used for all the simulation sections, and the root condition and seedling height data of all the ground cover plants planted in the simulation sections are measured through the measurement module during the cultivation, wherein the cultivation time of the ground cover plants before the first measurement is started is not less than 2 weeks, and the measurement period is 7 days, the measurement result is judged through the judgment module after one measurement is completed, and the judgment result is processed through the processing module, wherein the measurement result is that the root system has the elongation phenomenon and the seedling height data is increased, and the plant is judged to be alive, and the measurement result is that the root system has the elongation phenomenon, And if the seedling height data is not increased, judging that the plant survives, judging that the rest of the measurement results are dead plants, continuously cultivating the surviving plants if the plant survives, recording the measured data at the corresponding plant number, eradicating the dead plants if the plant death is judged, and simultaneously calculating the plant survival rate of each simulation interval through a calculation module.
5. The method for screening saline-alkali tolerant garden cover plants according to claim 1, wherein in S4, a professional draws a line graph of all measured data recorded by the same surviving plant through a drawing module, compares the drawn line graphs, selects the plant with the strongest stability in the same simulation interval as a plant gene preselection by comparing the comparison result, evaluates all the gene preselection plants through an evaluation module, screens out the plant with the best saline-alkali tolerant effect as a plant gene through evaluation, and performs large-scale propagation on the screened plant gene.
6. The method for screening saline-alkali tolerant garden cover plants according to claim 5, wherein before the large-scale propagation, the stem tips and anthers of the screened plant genes are trimmed by the trimming module, and after the trimming is completed, the screened plant genes are cut into small plants with the same size and soaked in 70% alcohol solution for 10-30 seconds, after the soaking is completed, the cut small plants are respectively inoculated into primary culture media by the culture module for culture, wherein the culture is performed by a professional for real-time observation and processing by observation results, wherein the observation results show that the cells start to differentiate to form callus, the subculture is performed, the observation results show that the cells start to differentiate to form callus, the culture is continued, and the professional performs real-time observation during the culture until the observation results show that the cells start to differentiate to form callus, and the observation is stopped, and after the subculture is completed, a professional selects half of the plants to perform strong seedling treatment, wherein the strong seedling treatment is to place the cultured plants outdoors for culture, plant the plants in the saline-alkali soil after the plants are adapted to the external environment, and simultaneously select the other half of the plants to perform secondary large-scale propagation.
7. The screening device for the saline-alkali tolerant garden ground cover plants comprises a simulation module, a calculation module, a control module, a cultivation module, a determination module, a judgment module, a processing module, a drawing module, an evaluation module, a trimming module and a cultivation module, and is characterized in that the simulation module is connected with the calculation module, the calculation module is connected with the control module, the control module is connected with the cultivation module, the cultivation module is connected with the determination module, the determination module is connected with the judgment module, the judgment module is connected with the processing module, the processing module is connected with the drawing module, the drawing module is connected with the evaluation module, the evaluation module is connected with the trimming module, and the trimming module is connected with the cultivation module.
8. The device for screening saline-alkali tolerant garden coverages according to claim 7, wherein the simulation module is used for simulating the soil condition of saline-alkali land, the calculation module is used for calculating the saline-alkali degree threshold value of the selected species of the coverages, the control module is used for controlling the living environment and the field condition required by the selected coverages, the cultivation module is used for cultivating the planted coverages, the determination module is used for determining the root condition and seedling height data of all coverages planted in the simulation area, the determination module is used for determining the determination result, the processing module is used for processing the determination result, the drawing module is used for drawing a line graph of all determination data recorded by the same survival plant, and the evaluation module is used for evaluating all pre-selected genetic plants, the pruning module is used for pruning the screened plant genes, and the culture module is used for performing large-scale propagation culture.
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